Bhi09G001801 (gene) Wax gourd (B227) v1

Overview
NameBhi09G001801
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionpolyphenol oxidase, chloroplastic-like
Locationchr9: 59746869 .. 59747414 (-)
RNA-Seq ExpressionBhi09G001801
SyntenyBhi09G001801
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGTACTTCCCGAGGATGATCCACATAGCTTTACACAACAAGCGAATGTTCATTGTGGTTATTGTAATGGGGCGTATGACCAAGTGGGGTTTTTAGTTGTGGTGCAAGTTCATAATTCGCGACTGTTTTTTCCATTTCATAGATTTTATCTTTAGTTTCATGAGAAGATATTGGGGGAGTTGATTGGGGATTCGAGTTTTGCTTTGCCGTTTTGGAATTATAATGCTACTGGTGGAATGAAAATGCCAGGGATATATGCGGAAGAGAAATCTTCGCTTTATGATGAATTACAGAATGAAAATCATTTGCCATCGAGATTGGTCGATTTGGATTTTGGTGGAGTTGATCCATCAAAATTTTCATCTACCCACACAATAAAAAACTTTAAGGCCATGGATGAAGAGGAGGAAAGGATGAAGGAAGTTGGCGGTGAGAAGGAGCAGGAGAAGGAGGCTGATCGTTTTTCTACCCGTGAGGATGAAGAAATCTATCGGCGAAGTTCAATTGAAATGAGGCAATTTGGGTATTTTAAAAAATTTTAA

mRNA sequence

ATGAAATTTCATGAGAAGATATTGGGGGAGTTGATTGGGGATTCGAGTTTTGCTTTGCCGTTTTGGAATTATAATGCTACTGGTGGAATGAAAATGCCAGGGATATATGCGGAAGAGAAATCTTCGCTTTATGATGAATTACAGAATGAAAATCATTTGCCATCGAGATTGGTCGATTTGGATTTTGGTGGAGTTGATCCATCAAAATTTTCATCTACCCACACAATAAAAAACTTTAAGGCCATGGATGAAGAGGAGGAAAGGATGAAGGAAGTTGGCGGTGAGAAGGAGCAGGAGAAGGAGGCTGATCGTTTTTCTACCCGTGAGGATGAAGAAATCTATCGGCGAAGTTCAATTGAAATGAGGCAATTTGGGTATTTTAAAAAATTTTAA

Coding sequence (CDS)

ATGAAATTTCATGAGAAGATATTGGGGGAGTTGATTGGGGATTCGAGTTTTGCTTTGCCGTTTTGGAATTATAATGCTACTGGTGGAATGAAAATGCCAGGGATATATGCGGAAGAGAAATCTTCGCTTTATGATGAATTACAGAATGAAAATCATTTGCCATCGAGATTGGTCGATTTGGATTTTGGTGGAGTTGATCCATCAAAATTTTCATCTACCCACACAATAAAAAACTTTAAGGCCATGGATGAAGAGGAGGAAAGGATGAAGGAAGTTGGCGGTGAGAAGGAGCAGGAGAAGGAGGCTGATCGTTTTTCTACCCGTGAGGATGAAGAAATCTATCGGCGAAGTTCAATTGAAATGAGGCAATTTGGGTATTTTAAAAAATTTTAA

Protein sequence

MKFHEKILGELIGDSSFALPFWNYNATGGMKMPGIYAEEKSSLYDELQNENHLPSRLVDLDFGGVDPSKFSSTHTIKNFKAMDEEEERMKEVGGEKEQEKEADRFSTREDEEIYRRSSIEMRQFGYFKKF
Homology
BLAST of Bhi09G001801 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 6.8e-18
Identity = 41/80 (51.25%), Postives = 54/80 (67.50%), Query Frame = 0

Query: 3   FHEKILGELIGDSSFALPFWNYNATGGMKMPGIYAEEKSSLYDELQNENHLPSRLVDLDF 62
           F EKILG+LI D +FALPFWN+++  GM +P IYA+ KS LYD+L++ NH P  LVDLD+
Sbjct: 211 FFEKILGKLINDPTFALPFWNWDSPAGMPLPAIYADPKSPLYDKLRSANHQPPTLVDLDY 270

Query: 63  GGVDPSKFSSTHTIKNFKAM 83
            G + +    T    N K M
Sbjct: 271 NGTEDNVSKETTINANLKIM 290

BLAST of Bhi09G001801 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 4.8e-16
Identity = 36/60 (60.00%), Postives = 48/60 (80.00%), Query Frame = 0

Query: 3   FHEKILGELIGDSSFALPFWNYNATGGMKMPGIYAEEKSSLYDELQNENHLPSRLVDLDF 62
           F+E+ILG LI D +FALPFWNY+A  GM++P IYA++ S LYDEL+N +H P  L+DL+F
Sbjct: 217 FYERILGSLINDPTFALPFWNYDAPDGMQLPTIYADKASPLYDELRNASHQPPTLIDLNF 276

BLAST of Bhi09G001801 vs. ExPASy Swiss-Prot
Match: Q9FRX6 (Aureusidin synthase OS=Antirrhinum majus OX=4151 GN=AS1 PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.2e-14
Identity = 33/64 (51.56%), Postives = 48/64 (75.00%), Query Frame = 0

Query: 3   FHEKILGELIGDSSFALPFWNYNATGGMKMPGIYAEEKSSLYDELQNENHLPSRLVDLDF 62
           F E+ILG+LI D++FALPFWNY++ GGM +P ++ +  SSLYD L++ NH P  +VDL++
Sbjct: 183 FFERILGKLINDTTFALPFWNYDSPGGMTIPSMFIDTNSSLYDSLRDSNHQPPTIVDLNY 242

Query: 63  GGVD 67
              D
Sbjct: 243 AFSD 246

BLAST of Bhi09G001801 vs. ExPASy Swiss-Prot
Match: Q9ZP19 (Polyphenol oxidase I, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-1 PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 2.0e-14
Identity = 34/64 (53.12%), Postives = 46/64 (71.88%), Query Frame = 0

Query: 3   FHEKILGELIGDSSFALPFWNYNATGGMKMPGIYAEEKSSLYDELQNENHLPSRLVDLDF 62
           F+E+ILG+LIGD SF LPFWN++  GGM +P    +  SSLYD  +N++HLP  +VDL +
Sbjct: 124 FYERILGKLIGDPSFGLPFWNWDNPGGMVLPDFLNDSTSSLYDSNRNQSHLPPVVVDLGY 183

Query: 63  GGVD 67
            G D
Sbjct: 184 NGAD 187

BLAST of Bhi09G001801 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 2.0e-14
Identity = 34/66 (51.52%), Postives = 47/66 (71.21%), Query Frame = 0

Query: 3   FHEKILGELIGDSSFALPFWNYNATGGMKMPGIYAEEKSSLYDELQNENHLPSRLVDLDF 62
           F+E+IL +LI D +FALP+W ++   GM MP IYA   SSLYDE +N  HLP  ++DLD+
Sbjct: 226 FNERILAKLIDDPTFALPYWAWDNPDGMYMPTIYASSPSSLYDEKRNAKHLPPTVIDLDY 285

Query: 63  GGVDPS 69
            G +P+
Sbjct: 286 DGTEPT 291

BLAST of Bhi09G001801 vs. ExPASy TrEMBL
Match: A0A0A0LXD0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073640 PE=3 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.8e-26
Identity = 58/66 (87.88%), Postives = 62/66 (93.94%), Query Frame = 0

Query: 3   FHEKILGELIGDSSFALPFWNYNATGGMKMPGIYAEEKSSLYDELQNENHLPSRLVDLDF 62
           FHEKILGELIGD SFALPFWNY+A  GMKMPG+YAEEKSSLYDEL+NENHLP RLVDLDF
Sbjct: 193 FHEKILGELIGDPSFALPFWNYDAPDGMKMPGMYAEEKSSLYDELRNENHLPPRLVDLDF 252

Query: 63  GGVDPS 69
           GGVDP+
Sbjct: 253 GGVDPN 258

BLAST of Bhi09G001801 vs. ExPASy TrEMBL
Match: A0A5D3DS75 (Polyphenol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00570 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 3.1e-26
Identity = 59/76 (77.63%), Postives = 64/76 (84.21%), Query Frame = 0

Query: 3   FHEKILGELIGDSSFALPFWNYNATGGMKMPGIYAEEKSSLYDELQNENHLPSRLVDLDF 62
           FHEKILGELIGDSSFALPFWNY+A GGMKMP +YAEEKSSLYDEL+NENHLP RLVDLDF
Sbjct: 193 FHEKILGELIGDSSFALPFWNYDAPGGMKMPRMYAEEKSSLYDELRNENHLPPRLVDLDF 252

Query: 63  GGVDPSKFSSTHTIKN 79
           GGVD         I++
Sbjct: 253 GGVDDPNIGDDAQIRS 268

BLAST of Bhi09G001801 vs. ExPASy TrEMBL
Match: A0A1S3B632 (polyphenol oxidase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103486180 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 3.1e-26
Identity = 59/76 (77.63%), Postives = 64/76 (84.21%), Query Frame = 0

Query: 3   FHEKILGELIGDSSFALPFWNYNATGGMKMPGIYAEEKSSLYDELQNENHLPSRLVDLDF 62
           FHEKILGELIGDSSFALPFWNY+A GGMKMP +YAEEKSSLYDEL+NENHLP RLVDLDF
Sbjct: 193 FHEKILGELIGDSSFALPFWNYDAPGGMKMPRMYAEEKSSLYDELRNENHLPPRLVDLDF 252

Query: 63  GGVDPSKFSSTHTIKN 79
           GGVD         I++
Sbjct: 253 GGVDDPNIGDDAQIRS 268

BLAST of Bhi09G001801 vs. ExPASy TrEMBL
Match: A0A1L5JHU3 (Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 3.5e-25
Identity = 55/68 (80.88%), Postives = 61/68 (89.71%), Query Frame = 0

Query: 1   MKFHEKILGELIGDSSFALPFWNYNATGGMKMPGIYAEEKSSLYDELQNENHLPSRLVDL 60
           + FHEKILGELIGD SFALPFWNY+A GGMKMP +YA+EKSSLYDEL+N NHLP  LVDL
Sbjct: 201 LHFHEKILGELIGDPSFALPFWNYDAPGGMKMPAMYADEKSSLYDELRNRNHLPPTLVDL 260

Query: 61  DFGGVDPS 69
           DFGGVDP+
Sbjct: 261 DFGGVDPT 268

BLAST of Bhi09G001801 vs. ExPASy TrEMBL
Match: A0A6J1CDF9 (polyphenol oxidase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111010674 PE=3 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 4.2e-23
Identity = 51/80 (63.75%), Postives = 63/80 (78.75%), Query Frame = 0

Query: 3   FHEKILGELIGDSSFALPFWNYNATGGMKMPGIYAEEKSSLYDELQNENHLPSRLVDLDF 62
           FHEKI+GELIGD +FALPFWNY+A  GM+MP IYA++KSSLYDEL+N+NHLP  LVDLD+
Sbjct: 54  FHEKIMGELIGDPTFALPFWNYDAPEGMRMPAIYADDKSSLYDELRNDNHLPPTLVDLDY 113

Query: 63  GGVDPSKFSSTHTIKNFKAM 83
           GGV+P+         N + M
Sbjct: 114 GGVEPTIDDEAQIRSNLRVM 133

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
P433096.8e-1851.25Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1[more]
Q062154.8e-1660.00Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1[more]
Q9FRX61.2e-1451.56Aureusidin synthase OS=Antirrhinum majus OX=4151 GN=AS1 PE=1 SV=1[more]
Q9ZP192.0e-1453.13Polyphenol oxidase I, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-1 PE=1 SV=1[more]
P433112.0e-1451.52Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LXD01.8e-2687.88Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073640 PE=3 SV=1[more]
A0A5D3DS753.1e-2677.63Polyphenol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G0... [more]
A0A1S3B6323.1e-2677.63polyphenol oxidase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103486180 P... [more]
A0A1L5JHU33.5e-2580.88Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
A0A6J1CDF94.2e-2363.75polyphenol oxidase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 82..102
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..109
NoneNo IPR availablePANTHERPTHR11474:SF102POLYPHENOL OXIDASE, CHLOROPLASTIC-LIKEcoord: 2..82
NoneNo IPR availablePANTHERPTHR11474TYROSINASE FAMILY MEMBERcoord: 2..82
IPR008922Uncharacterised domain, di-copper centreGENE3D1.10.1280.10coord: 1..123
e-value: 1.9E-15
score: 59.2
IPR008922Uncharacterised domain, di-copper centreSUPERFAMILY48056Di-copper centre-containing domaincoord: 2..82

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi09M001801Bhi09M001801mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046148 pigment biosynthetic process
molecular_function GO:0004097 catechol oxidase activity
molecular_function GO:0046872 metal ion binding