Bhi09G001739 (gene) Wax gourd (B227) v1

Overview


Name	Bhi09G001739
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Cytochrome P450
Location	chr9: 57707427 .. 57707997 (+)
RNA-Seq Expression	Bhi09G001739
Synteny	Bhi09G001739

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGTGCTGCCTTACGGTCCACTCTGGCGTCATGTTCGCAAAATAGTCGCGTTTGAACTCCTCTCAAACCACCGTCTTGAGCAGCTCAGGCACATTCGAATTTCTGAAGTTCAGAGTAGTATTAAGAATTTGTATGAATTATGGGTCGATAGAAGTGAAGAAAAACTGCTGGTGGACATGACGATGTGGTTTGGAGATATAGCCCGAAATACAATCTTCAGGATGGTGGTTGGAAAGCGATTCTCGGCCACCGTCGACAAGAGTGCTAATGAAGAGTATCGGAAGGTGGTGAGCGATTACTTTGAAATGTTTGGAGCTTTCGTTCCATCCGATTCGTTACCATTTCTAAAGCGACTGGATTTGGGAGGACATGAAAAGGCGATGAAAAAGACGAAGAAGGTGCTAGATCAAGTGCTTGATAAATGGATCGAAGAGCACCGAAATAAGAGGAATTTTGATCATGAAGTTGAGATAGAAGCGAATGACTTTATGGATGTGATGCTCTCTGCTCTTAAAGATGAGGATCAACTTTCTAACTACGATCATTATACAATCACCAAAGCTACAT

mRNA sequence

ATGGGTGTGCTGCCTTACGGTCCACTCTGGCGTCATGTTCGCAAAATAGTCGCGTTTGAACTCCTCTCAAACCACCGTCTTGAGCAGCTCAGGCACATTCGAATTTCTGAAGTTCAGAGTAGTATTAAGAATTTGTATGAATTATGGGTCGATAGAAGTGAAGAAAAACTGCTGGTGGACATGACGATGTGGTTTGGAGATATAGCCCGAAATACAATCTTCAGGATGGTGGTTGGAAAGCGATTCTCGGCCACCGTCGACAAGAGTGCTAATGAAGAGTATCGGAAGGTGGTGAGCGATTACTTTGAAATGTTTGGAGCTTTCGTTCCATCCGATTCGTTACCATTTCTAAAGCGACTGGATTTGGGAGGACATGAAAAGGCGATGAAAAAGACGAAGAAGGTGCTAGATCAAGTGCTTGATAAATGGATCGAAGAGCACCGAAATAAGAGGAATTTTGATCATGAAGTTGAGATAGAAGCGAATGACTTTATGGATGTGATGCTCTCTGCTCTTAAAGATGAGGATCAACTTTCTAACTACGATCATTATACAATCACCAAAGCTACAT

Coding sequence (CDS)

ATGGGTGTGCTGCCTTACGGTCCACTCTGGCGTCATGTTCGCAAAATAGTCGCGTTTGAACTCCTCTCAAACCACCGTCTTGAGCAGCTCAGGCACATTCGAATTTCTGAAGTTCAGAGTAGTATTAAGAATTTGTATGAATTATGGGTCGATAGAAGTGAAGAAAAACTGCTGGTGGACATGACGATGTGGTTTGGAGATATAGCCCGAAATACAATCTTCAGGATGGTGGTTGGAAAGCGATTCTCGGCCACCGTCGACAAGAGTGCTAATGAAGAGTATCGGAAGGTGGTGAGCGATTACTTTGAAATGTTTGGAGCTTTCGTTCCATCCGATTCGTTACCATTTCTAAAGCGACTGGATTTGGGAGGACATGAAAAGGCGATGAAAAAGACGAAGAAGGTGCTAGATCAAGTGCTTGATAAATGGATCGAAGAGCACCGAAATAAGAGGAATTTTGATCATGAAGTTGAGATAGAAGCGAATGACTTTATGGATGTGATGCTCTCTGCTCTTAAAGATGAGGATCAACTTTCTAACTACGATCATTATACAATCACCAAAGCTACAT

Protein sequence

MGVLPYGPLWRHVRKIVAFELLSNHRLEQLRHIRISEVQSSIKNLYELWVDRSEEKLLVDMTMWFGDIARNTIFRMVVGKRFSATVDKSANEEYRKVVSDYFEMFGAFVPSDSLPFLKRLDLGGHEKAMKKTKKVLDQVLDKWIEEHRNKRNFDHEVEIEANDFMDVMLSALKDEDQLSNYDHYTITKAT

Homology

[Close]

BLAST of Bhi09G001739 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 147.1 bits (370), Expect = 1.4e-35
Identity = 83/198 (41.92%), Postives = 117/198 (59.09%), Query Frame = 0


Query: 2   GVLPYGPLWRHVRKIVAFELLSNHRLEQLRHIRISEVQSSIKNLYELWVDRSEEK-LLVD 61
           G  PY   WR +RKI   ELLSN RL+ L+H+R+SE+   +K+LY LW      K ++VD
Sbjct: 119 GFAPYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVD 178

Query: 62  MTMWFGDIARNTIFRMVVGKRF---SATVDKSANEE---YRKVVSDYFEMFGAFVPSDSL 121
           +  W  D+  N I RMV GKR+     +V     EE    +K ++ +F + G F  SD+ 
Sbjct: 179 LKSWLEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAF 238

Query: 122 PFLKRLDLGGHEKAMKKTKKVLDQVLDKWIEEHRNKRNFDHEVEIEANDFMDVMLSALKD 181
           P L   DL GHEK MK+T   LD +L++WIE HR +R F    E + +DF+DVM+S L +
Sbjct: 239 PTLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKEND-SDFIDVMMS-LAE 298

Query: 182 EDQLSN--YDHYTITKAT 191
           + +LS+  YD  T  K+T
Sbjct: 299 QGKLSHLQYDANTSIKST 314

[Close]

BLAST of Bhi09G001739 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 142.1 bits (357), Expect = 4.5e-34
Identity = 77/192 (40.10%), Postives = 114/192 (59.38%), Query Frame = 0


Query: 2   GVLPYGPLWRHVRKIVAFELLSNHRLEQLRHIRISEVQSSIKNLYELWVDR-SEEKLLVD 61
           G  PY   WR +RKI   ELLSN RL+ L+H+R+SE+   +++LY LWV +   E ++VD
Sbjct: 119 GFAPYSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVMVD 178

Query: 62  MTMWFGDIARNTIFRMVVGKRF-----SATVDKSANEEYRKVVSDYFEMFGAFVPSDSLP 121
           +  W  D++ N + RMV GKR+      +  D     + RK V+++F + G F  SD+ P
Sbjct: 179 LKSWLEDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFP 238

Query: 122 FLKRLDLGGHEKAMKKTKKVLDQVLDKWIEEHRNKRNFDHEVEIEANDFMDVMLSALKDE 181
            L   D  GHEK MK+T + LD +L++WIE HR +R      +   +DF+DVMLS L ++
Sbjct: 239 KLGWFDFQGHEKEMKQTGRELDVILERWIENHRQQRKVS-GTKHNDSDFVDVMLS-LAEQ 298

Query: 182 DQLSNYDHYTIT 188
            + S+  H  IT
Sbjct: 299 GKFSHLQHDAIT 308

[Close]

BLAST of Bhi09G001739 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 137.5 bits (345), Expect = 1.1e-32
Identity = 78/190 (41.05%), Postives = 115/190 (60.53%), Query Frame = 0


Query: 10  WRHVRKIVAFELLSNHRLEQLRHIRISEVQSSIKNLYELWVDR-SEEKLLVDMTMWFGDI 69
           W  +RKI   ELLSN RL+ L ++R+SE+   +K+LY LWV +   E ++VD+  W  D+
Sbjct: 115 WLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSEPVMVDLKSWLEDM 174

Query: 70  ARNTIFRMVVGKRFSATVDKSANE------EYRKVVSDYFEMFGAFVPSDSLPFLKRLDL 129
             N I RMV GKR+       ++E      ++RK ++ +F + G F  SD+ P L  LDL
Sbjct: 175 IANMIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWLDL 234

Query: 130 GGHEKAMKKTKKVLDQVLDKWIEEHRNKRNFDHEVEIEANDFMDVMLSALKDEDQLSN-- 189
            GHEK MK+T++ LD +L++WIE HR +R      +   +DF+DVMLS L ++ +LS+  
Sbjct: 235 QGHEKEMKQTRRELDVILERWIENHRQQRKVS-GTKHNDSDFVDVMLS-LAEQGKLSHLQ 294

Query: 190 YDHYTITKAT 191
           YD  T  K T
Sbjct: 295 YDANTCIKTT 302

[Close]

BLAST of Bhi09G001739 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 94.0 bits (232), Expect = 1.4e-19
Identity = 66/190 (34.74%), Postives = 96/190 (50.53%), Query Frame = 0


Query: 5   PYGPLWRHVRKIVAFELLSNHRLEQLRHIRISEVQSSIKNLYELWVDRSEEK--LLVDMT 64
           PYG  WR +RKI   EL S   ++     R  E   + + LY  W  R + K  +LVDM 
Sbjct: 122 PYGLYWREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVDMK 181

Query: 65  MWFGDIARNTIFRMVVGKRFSATVDKSANEEYR---KVVSDYFEMFGAFVPSDSLPFLKR 124
             F D+  N    MV GKR+         +E R   K++ ++ + F  F+ SD  P L  
Sbjct: 182 QEFIDLTANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGF 241

Query: 125 LDLGGHEKAMKKTKKVLDQVLDKWIEEHRNKRNFDHEVEIEANDFMDVMLSALKDEDQLS 184
           LD    ++ MK+T K LD+V + WIEEH+NKR+ DH      ND++D+++  L  +    
Sbjct: 242 LD-WKTKRGMKRTAKGLDKVAEGWIEEHKNKRS-DH--GRSENDYLDILIKILGQDKIPG 301

Query: 185 NYDHYTITKA 190
             D +T  KA
Sbjct: 302 LSDTHTKIKA 307

[Close]

BLAST of Bhi09G001739 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 88.6 bits (218), Expect = 5.9e-18
Identity = 63/186 (33.87%), Postives = 93/186 (50.00%), Query Frame = 0


Query: 5   PYGPLWRHVRKIVAFELLSNHRLEQLRHIRISEVQSSIKNLYELWVDRSEEKLLVDMTMW 64
           PYG  WR +RKIV   L SNH +E L HIR SEV + IK+LY+     +    +V + M 
Sbjct: 128 PYGDYWRELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLYK----GNGGTSIVKIDML 187

Query: 65  FGDIARNTIFRMVVGKRFSATVDKSANEEYRKVVSDYFEMFGAFVPSDSLPFLKRLDLGG 124
           F  +  N I R +VGKR       S    Y++ +     +    +  D +P+L  LD   
Sbjct: 188 FEFLTFNIILRKMVGKRIGFGEVNSDEWRYKEALKHCEYLAVIPMIGDVIPWLGWLDFAK 247

Query: 125 HEKAMKKTKKVLDQVLDKWIEEHRNKRNFDHEVEIEANDFMDVMLSALKDEDQLSNYDHY 184
           + + MK+  K LD V  KW+ EH  KR+     + +    MD++L  L ++  +S +   
Sbjct: 248 NSQ-MKRLFKELDSVNTKWLHEHLKKRS--RNEKDQERTIMDLLLDILPEDIVISGHVRD 306

Query: 185 TITKAT 191
            I KAT
Sbjct: 308 VIVKAT 306

[Close]

BLAST of Bhi09G001739 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 5.4e-40
Identity = 87/196 (44.39%), Postives = 125/196 (63.78%), Query Frame = 0


Query: 1   MGVLPYGPLWRHVRKIVAFELLSNHRLEQLRHIRISEVQSSIKNLYELWVDRSEEK---L 60
           +G+ PYGP WR +RKIV FE LSN R+EQ  HIR+SEV++SIK L+++W + ++ +    
Sbjct: 123 VGLAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYT 182

Query: 61  LVDMTMWFGDIARNTIFRMVVGKRFSATV---DKSANEEYRKVVSDYFEMFGAFVPSDSL 120
           LVD+  W   +  N + RMVVGKR+   +    K   + + K + ++  + G F  +D +
Sbjct: 183 LVDIKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGV 242

Query: 121 PFLKRLDLGGHEKAMKKTKKVLDQVLDKWIEEHRNKRNFDHEVEIEANDFMDVMLSALKD 180
           P L+ LDLGGHEKAMK   K +D++L +W+EEHR K+     VE +  DFMDVM+SAL  
Sbjct: 243 PCLRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQKKLLGENVESD-RDFMDVMISALNG 302

Query: 181 EDQLSNYDHYTITKAT 191
             Q+  +D  TI KAT
Sbjct: 303 A-QIGAFDADTICKAT 316

[Close]

BLAST of Bhi09G001739 vs. ExPASy Swiss-Prot
Match: H2DH24 (Cytochrome P450 CYP82D47 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 3.5e-39
Identity = 88/191 (46.07%), Postives = 127/191 (66.49%), Query Frame = 0


Query: 5   PYGPLWRHVRKIVAFELLSNHRLEQLRHIRISEVQSSIKNLYELWVDRSE--EKLLVDMT 64
           PY   W  +RKI + +LLS+ RLE L+H+RISE++ S+K LY++W  + +   ++LV+M 
Sbjct: 6   PYSSFWVELRKITSLQLLSSRRLELLKHVRISEMEISMKQLYKIWSKKKDGSGRVLVEMK 65

Query: 65  MWFGDIARNTIFRMVVGKRFSATVDKSANEE---YRKVVSDYFEMFGAFVPSDSLPFLKR 124
             FGD+  N + RMV GKR+    + + +EE    R+V  ++F + G FV +DSLPFL+ 
Sbjct: 66  KLFGDLTLNVVLRMVAGKRYFGGGNANDDEEARRCRRVWREFFRLHGVFVVADSLPFLRW 125

Query: 125 LDLGGHEKAMKKTKKVLDQVLDKWIEEHRNKRNFDHEVEIEANDFMDVMLSALKDEDQLS 184
           LDLGG+EKAMKKT K +D ++  W+EEHR KRN   E   +  DFMDVMLSA+K+ D L 
Sbjct: 126 LDLGGYEKAMKKTAKEMDNIVSGWLEEHRMKRNSSDEDNTQ-QDFMDVMLSAVKNVD-LC 185

Query: 185 NYDHYTITKAT 191
            +D   + K+T
Sbjct: 186 GFDADVVIKST 194

[Close]

BLAST of Bhi09G001739 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 5.0e-38
Identity = 91/193 (47.15%), Postives = 119/193 (61.66%), Query Frame = 0


Query: 1   MGVLPYGPLWRHVRKIVAFELLSNHRLEQLRHIRISEVQSSIKNLYELWVDRSEEKLLVD 60
           +GV PYGP WR +RKI+  E LSN RL+ L+H+  SE+  S K LY+LW  ++ +  LVD
Sbjct: 121 LGVAPYGPYWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQNIDFCLVD 180

Query: 61  MTMWFGDIARNTIFRMVVGKRFSATVDKSANEEYR-----KVVSDYFEMFGAFVPSDSLP 120
           M  W  D+  N   +MVVGKRF  +   SA EE       K + + F + G+FV SD LP
Sbjct: 181 MKQWLADLTLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLP 240

Query: 121 FLKRLDLGGHEKAMKKTKKVLDQVLDKWIEEHRNKRNFDHEVEIEANDFMDVMLSALKDE 180
           +L+ LDLGGHEK MK+T K LD +   W++EH+ KR      E +  DFMDVMLS L +E
Sbjct: 241 YLRWLDLGGHEKEMKRTVKELDILFKGWLDEHKRKR-LSGGKEDDDQDFMDVMLSIL-EE 300

Query: 181 DQLSNYDHYTITK 189
            +L N D  TI K
Sbjct: 301 SKLGN-DVDTINK 310

[Close]

BLAST of Bhi09G001739 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 1.9e-37
Identity = 86/191 (45.03%), Postives = 121/191 (63.35%), Query Frame = 0


Query: 3   VLPYGPLWRHVRKIVAFELLSNHRLEQLRHIRISEVQSSIKNLYELWVDRSEEK--LLVD 62
           V PYGP WR +RKI+  E+LS+ R+EQL+ +R+SEVQ+SI  LY++W  +  E     V+
Sbjct: 127 VAPYGPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDYASVE 186

Query: 63  MTMWFGDIARNTIFRMVVGKRF-SATVDKSANEEYRKVVSDYFEMFGAFVPSDSLPFLKR 122
           +  WF     N + RMVVGKRF SAT      E+  K V ++  + G F   D++P+L+ 
Sbjct: 187 LKQWFAQPIFNMVLRMVVGKRFLSATATDEKAEKCVKAVDEFMRLAGVFTVGDAIPYLRW 246

Query: 123 LDLGGHEKAMKKTKKVLDQVLDKWIEEHRNKRNFDHEVEIEANDFMDVMLSALKDEDQLS 182
           LD GG+EKAMK+T K LD ++ +W+EEHR KR     V+  A DFM+VMLS+L D   + 
Sbjct: 247 LDFGGYEKAMKETAKELDVMISEWLEEHRQKRALGEGVD-GAQDFMNVMLSSL-DGKTID 306

Query: 183 NYDHYTITKAT 191
             D  T+ K+T
Sbjct: 307 GIDADTLIKST 315

[Close]

BLAST of Bhi09G001739 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 155.2 bits (391), Expect = 7.3e-37
Identity = 88/215 (40.93%), Postives = 120/215 (55.81%), Query Frame = 0


Query: 1   MGVLPYGPLWRHVRKIVAFELLSNHRLEQLRHIRISEVQSSIKNLYELWVDR-------- 60
           +G  PYG  WR +RKIV  E+LSN R+E L HIR+SEVQ+SIK L  +W ++        
Sbjct: 120 IGWAPYGAYWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLL 179

Query: 61  -------------SEEKLLVDMTMWFGDIARNTIFRMVVGKRFSATVDKSANEEYRKV-- 120
                        + + + V++  WF  +  N + RMVVGKR    VD    EE ++   
Sbjct: 180 DDTKSSSTNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLE 239

Query: 121 -VSDYFEMFGAFVPSDSLPFLKRLDLGGHEKAMKKTKKVLDQVLDKWIEEHRNKRNFDHE 180
            + D+  + G F   D +PFLK LDLGGHEK MKK  K  D +L++W+EEHR K+    E
Sbjct: 240 NIRDFMRLIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGLGSE 299

Query: 181 VEIEA-NDFMDVMLSALKDEDQLSNYDHYTITKAT 191
            ++    DFMD ML  LKD+  +  +D  TI KAT
Sbjct: 300 DKVVGERDFMDAMLLVLKDK-PIEGFDVDTIIKAT 333

[Close]

BLAST of Bhi09G001739 vs. ExPASy TrEMBL
Match: A0A6J1F697 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442562 PE=3 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 9.8e-61
Identity = 125/195 (64.10%), Postives = 149/195 (76.41%), Query Frame = 0


Query: 1   MGVLPYGPLWRHVRKIVAFELLSNHRLEQLRHIRISEVQSSIKNLYELWV----DRSEEK 60
           MG+ PYGP WRHVRK+   ELL+N RLEQL+HIR+ EV+SSIK LYE+ V    + S E 
Sbjct: 126 MGLSPYGPHWRHVRKLATLELLTNQRLEQLQHIRVFEVRSSIKKLYEVCVKGRNNGSNEG 185

Query: 61  LLVDMTMWFGDIARNTIFRMVVGKRFSATVDKSANEEYRKVVSDYFEMFGAFVPSDSLPF 120
           +LV+M  WFGDI  NTIFR+VVGKRFS  V+ S NEEYR  + D+FE FG FVPSDS PF
Sbjct: 186 VLVEMKTWFGDITLNTIFRIVVGKRFSTAVEGSGNEEYRTALRDFFEWFGVFVPSDSFPF 245

Query: 121 LKRLDLGGHEKAMKKTKKVLDQVLDKWIEEHRNKRNFDHEVEIE-ANDFMDVMLSALKDE 180
           LK LDLGGHEKAMK T KVLD+V DKW++EHR K+    E ++E   DFMDVMLS +KD+
Sbjct: 246 LKWLDLGGHEKAMKNTAKVLDEVFDKWLQEHRQKKKNLGEGKMEDPPDFMDVMLSNVKDD 305

Query: 181 DQLSNYDHYTITKAT 191
            QLSNYD +T+TKAT
Sbjct: 306 GQLSNYDAHTVTKAT 320

[Close]

BLAST of Bhi09G001739 vs. ExPASy TrEMBL
Match: A0A5A7SU10 (Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold156G00630 PE=3 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.7e-60
Identity = 122/191 (63.87%), Postives = 152/191 (79.58%), Query Frame = 0


Query: 2   GVLPYGPLWRHVRKIVAFELLSNHRLEQLRHIRISEVQSSIKNLYELWVDRSEEKLLVDM 61
           G+ PYGP WRHVRK+   ELLSN+RLE+L+HIR+SEVQ+S+KNLYEL + ++++  LV+M
Sbjct: 128 GLSPYGPHWRHVRKLAMLELLSNYRLEKLQHIRVSEVQTSMKNLYELCL-KNKKNALVEM 187

Query: 62  TMWFGDIARNTIFRMVVGKRFSATVDKS--ANEEYRKVVSDYFEMFGAFVPSDSLPFLKR 121
             WFGDI  NTI R+VVGK+FS  VD S   NEEYRK + D+FE FG FVPSDS PFLK 
Sbjct: 188 KTWFGDITLNTISRIVVGKKFSTAVDASNNENEEYRKALRDFFEWFGVFVPSDSFPFLKW 247

Query: 122 LDLGGHEKAMKKTKKVLDQVLDKWIEEHRNKRNFDHEVEIEANDFMDVMLSALKDEDQLS 181
           LDLGGHEKAMKKT KVLD+V DKWI++H+N      EV++E +DFMDVMLS ++D+ QLS
Sbjct: 248 LDLGGHEKAMKKTAKVLDEVFDKWIQDHKNNLG---EVKMEEHDFMDVMLSNVRDDGQLS 307

Query: 182 NYDHYTITKAT 191
            YD +T+TKAT
Sbjct: 308 KYDAHTVTKAT 314

[Close]

BLAST of Bhi09G001739 vs. ExPASy TrEMBL
Match: A0A1S3CM78 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502573 PE=3 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.7e-60
Identity = 122/191 (63.87%), Postives = 152/191 (79.58%), Query Frame = 0


Query: 2   GVLPYGPLWRHVRKIVAFELLSNHRLEQLRHIRISEVQSSIKNLYELWVDRSEEKLLVDM 61
           G+ PYGP WRHVRK+   ELLSN+RLE+L+HIR+SEVQ+S+KNLYEL + ++++  LV+M
Sbjct: 128 GLSPYGPHWRHVRKLAMLELLSNYRLEKLQHIRVSEVQTSMKNLYELCL-KNKKNALVEM 187

Query: 62  TMWFGDIARNTIFRMVVGKRFSATVDKS--ANEEYRKVVSDYFEMFGAFVPSDSLPFLKR 121
             WFGDI  NTI R+VVGK+FS  VD S   NEEYRK + D+FE FG FVPSDS PFLK 
Sbjct: 188 KTWFGDITLNTISRIVVGKKFSTAVDASNNENEEYRKALRDFFEWFGVFVPSDSFPFLKW 247

Query: 122 LDLGGHEKAMKKTKKVLDQVLDKWIEEHRNKRNFDHEVEIEANDFMDVMLSALKDEDQLS 181
           LDLGGHEKAMKKT KVLD+V DKWI++H+N      EV++E +DFMDVMLS ++D+ QLS
Sbjct: 248 LDLGGHEKAMKKTAKVLDEVFDKWIQDHKNNLG---EVKMEEHDFMDVMLSNVRDDGQLS 307

Query: 182 NYDHYTITKAT 191
            YD +T+TKAT
Sbjct: 308 KYDAHTVTKAT 314

[Close]

BLAST of Bhi09G001739 vs. ExPASy TrEMBL
Match: A0A0A0LG04 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852630 PE=3 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 6.3e-60
Identity = 121/196 (61.73%), Postives = 152/196 (77.55%), Query Frame = 0


Query: 1   MGVLPYGPLWRHVRKIVAFELLSNHRLEQLRHIRISEVQSSIKNLYELWV---DRSEEKL 60
           +G+ PYGP WRHVRK+   ELL++HRL++L+HIR+SEVQ+SIKNLYEL +     +E+  
Sbjct: 133 LGLSPYGPHWRHVRKLTMLELLTSHRLQKLQHIRVSEVQTSIKNLYELCLKNKKNNEKNA 192

Query: 61  LVDMTMWFGDIARNTIFRMVVGKRFSATVDKS---ANEEYRKVVSDYFEMFGAFVPSDSL 120
           LV+M  WFGDI  NTI R+VVGK+FS  VD S    NEEYRK + D+FE FG FVPSDS 
Sbjct: 193 LVEMKTWFGDITLNTISRIVVGKQFSTAVDVSNTNENEEYRKALRDFFEWFGVFVPSDSF 252

Query: 121 PFLKRLDLGGHEKAMKKTKKVLDQVLDKWIEEHRNKRNFDHEVEIEANDFMDVMLSALKD 180
           PFLK LDLGGHEKAMKKT +VLD+V DKWI+EH+ K+N    V++E +DFMDVMLS ++D
Sbjct: 253 PFLKWLDLGGHEKAMKKTAQVLDEVFDKWIQEHQQKKNNLGAVKMEEHDFMDVMLSNVRD 312

Query: 181 EDQLSNYDHYTITKAT 191
           + QLS YD + +TKAT
Sbjct: 313 DGQLSKYDAHIVTKAT 328

[Close]

BLAST of Bhi09G001739 vs. ExPASy TrEMBL
Match: A0A6J1J4B0 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111481116 PE=3 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 2.4e-59
Identity = 119/190 (62.63%), Postives = 145/190 (76.32%), Query Frame = 0


Query: 1   MGVLPYGPLWRHVRKIVAFELLSNHRLEQLRHIRISEVQSSIKNLYELWVDRSEEKLLVD 60
           MG+ PYG  WRHVRK+   EL +NHRL+QL+H+R+SEV+SSIK LYEL     +EK LVD
Sbjct: 128 MGLSPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEVESSIKKLYEL-CGNGKEKALVD 187

Query: 61  MTMWFGDIARNTIFRMVVGKRFSATVDKSANEEYRKVVSDYFEMFGAFVPSDSLPFLKRL 120
           M  WFGDI  NTIFR+VVGKRFS  V+   NEEYRK + ++FE FG FVPSDS PFLK L
Sbjct: 188 MKTWFGDITLNTIFRVVVGKRFSTAVEGGGNEEYRKALREFFEWFGVFVPSDSFPFLKWL 247

Query: 121 DLGGHEKAMKKTKKVLDQVLDKWIEEHRNKRNFDHEVEIEANDFMDVMLSALKDEDQLSN 180
           DLGGHEKAMKKT +VLD+VLDKW+EE R  R  D E +   +DFMDVMLS +KD ++LS+
Sbjct: 248 DLGGHEKAMKKTAEVLDEVLDKWVEERRKMRASD-EAKTAEHDFMDVMLSGVKDYEELSS 307

Query: 181 YDHYTITKAT 191
           Y+  T+ K+T
Sbjct: 308 YEANTVIKST 315

The following BLAST results are available for this feature:

BLAST of Bhi09G001739 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31940.1	1.4e-35	41.92	cytochrome P450, family 82, subfamily C, polypeptide 4	[more]
AT4G31970.1	4.5e-34	40.10	cytochrome P450, family 82, subfamily C, polypeptide 2	[more]
AT4G31950.1	1.1e-32	41.05	cytochrome P450, family 82, subfamily C, polypeptide 3	[more]
AT2G25160.1	1.4e-19	34.74	cytochrome P450, family 82, subfamily F, polypeptide 1	[more]
AT3G25180.1	5.9e-18	33.87	cytochrome P450, family 82, subfamily G, polypeptide 1	[more]

BLAST of Bhi09G001739 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49858	5.4e-40	44.39	Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1	[more]
H2DH24	3.5e-39	46.07	Cytochrome P450 CYP82D47 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]
A0A0N7F297	5.0e-38	47.15	Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP...	[more]
O49859	1.9e-37	45.03	Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1	[more]
Q43068	7.3e-37	40.93	Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2	[more]

BLAST of Bhi09G001739 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F697	9.8e-61	64.10	cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442562 PE=3...	[more]
A0A5A7SU10	1.7e-60	63.87	Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3CM78	1.7e-60	63.87	cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502573 PE=3 SV=1	[more]
A0A0A0LG04	6.3e-60	61.73	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852630 PE=3 SV=1	[more]
A0A6J1J4B0	2.4e-59	62.63	cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111481116 PE=3 S...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..188 e-value: 6.9E-25 score: 89.5
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 3..178
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 6..180 e-value: 1.8E-17 score: 63.2
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 2..190
None	No IPR available	PANTHER	PTHR47947:SF14	P450, PUTATIVE-RELATED	coord: 2..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M001739	Bhi09M001739	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen