Bhi09G001660 (gene) Wax gourd (B227) v1

Overview
NameBhi09G001660
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionindole-3-acetic acid-amido synthetase GH3.6
Locationchr9: 54804265 .. 54807912 (-)
RNA-Seq ExpressionBhi09G001660
SyntenyBhi09G001660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTATGCTTCTTTTTTTCCTCCTCACATCAAAAATCCCACCATCATTTCTTAAAAAAACCACACACAAACACACAATCTTCTCTTAATTACTCTTCTTTCCCTCTCTCTCTTTTTTTCAACTTTCCAAAAGACTATCCTTTTCCAACAACATGCCTGAAGAAACTCCTCAAAAGTCCTCATTTCTCAGCTCTACCACTGAATACTACAAACTGTTAGAGAAAAACAAGAAGACTCTTCAATTCATTGAAGATGTCACTGCCAACGCCGACCATGTCCAGCGTCGTGTCCTCACCGAGATCCTCACCCGCAATGCCGATGTCGAGTACCTCCGTCGACACGGCCTCGGTGGTGCCACCGACCGTAAGACCTTCAAAAAAACCCTTCCTCTCATCTCCTATGAGGATATTCAACCTGATATCAACCGTATTGCCAATGGGGACTCCTCTCCCATCCTCTGTTCCTCTCCCATTTCTGAGTTTCTCACCAGGTGGGTCTCTCTTTTTCCTCTTCCCCTTTTTTCCCTTAAATGGGTCTCTCTCTTTTTCTTGTTTTTGCCTTCAAATATCATTCAAAAATACTTAATTTACCATAACTAAATATCTGATTTTTAAATTGTTATAAACGTCATTTGTTTAACGGTAATCCATCAACTTAAATTTTTAGAAATTTATCCAACTTAAGCTTTTGGGATAGGTTATATTTTAATACAAACCCAAAATAAGATTTTTTTTTTTTTTTTTTAGTTTCCTTATGACTTAACTTTATCGAAAATTTTCTATTTTTAAATTGAACTGGGTAAATGTCATCTGGTTATTGAAATCCATCAGTAACAAAGAAATGACTTTTTTTAAATTAAAAAAAATCCATCAACTCAAACTTTTAAGTTCAGCAATACTCTCAACACGAACCCAAAACAAGAGGTGTCATCATATAGAAACCGCTAAAAACCATCATTTGTTTATAATCACACACTGCAAATGGATTCACATTCTCTCTTTCGAATTAAGCTTTTGAGTTTATGGACGATGATTATAAATTAACCGATAGTTGGTTCGTTCGTTCGTTCGTTCATCTCCAGCTCGGGTACATCGGGAGGAGAGAGAAAGCTGATGCCAACAATAGAAGAAGAGCTAGAAAGAAGATCATCACTCTACAGCTTACTAATGCCAGTAATGAGCCAATTCATAGAAGGCTTAGAGAAAGGCAAAGGAATGTACTTTCTATTCATAAAATCAGAATCAAAAACCCCAAGTGGCTTACTAGCTCGCCCTGTTTTAACAAGCTATTACAAAAGCTCCCATTTCAAACACCGTCCATTTGACCCATACACAAACTACACAAGCCCAAACCAAGCCATCCTCTGTTCCGACTCTTACCAAAGCATGTACGCTCAGCTCCTCTGTGGCCTCCTCCACCGCACCGACGTCCTCCGTGTCGGCGCCGTCTTCGCCTCGGGCTTCATCCGAGCCATCCGGTTCTTGGAAAAGCACTTCCCACTCCTCTGCCACGACATTCGAACAGGGACTCTGAATTCAGAGATTACAGATCAGGGGATCCGTGAATCGTTGAGGAAAATTCTGAAACCGGACCCGAAACTGGCGGATTTTGTGGAAGGGGAATGTGGGAAGAAATGTTGGGGAGGAATTATTAGTAGGATATGGCCGAATACGAAGTATGTGGATGTGATTGTAACAGGGACAATGTCGCAGTACATAAAGACATTGGATTATTACAGTAATGGGTTGCCGATTGTTTGTACAATGTATGCTTCTTCGGAGTGTTATTTTGGAGTGAATTTGAAGCCGATGTGTAAACCAGAGGAAGTGGCGTATACGCTTATACCAACAATGGCGTATTTTGAGTTTCTTCCCGTGAATAGGAGTAAAGGGTTGAGTGATAAAGAGTTGCAGAAGGAGCTTGTGGATCTTGTTGATGTTCAGCTTGGTCAGGAGTATGAGCTCGTCGTTACCACCTATGCAGGTCAGGACCCTTTTATTTTTTAATTTTTATTTATTTATTTATTTGTTGTTGAGAATCGACAAAAAGGTGTTTAATGGATTTCAATTTTATATCTAGTAGATCGTTAAAATTCAAAAGATTTAATAACTTTATATTAAAATTCGATAAAATTTAATAACTTACTAGAAGCTTTTTCTTAGTAGAAAGTAAACATTTTGTTTGATAATCTTTTTGTTTTTAAATTTTTTATTTTTGAAAAGTGTACATAGTTTTTTTACCATGGTTTTTTTTAAGAAAATGTTTTAATACTTTGCCAAATTCCACGAATAAATTCAAGTTTTTGAAAACATTTTTTTAATTTTTAAAATTTGGATTTTGACAAAAAAAAAAAAATAATAAATAAATAAAGGAATTCGTAAGTACAACCAGTTTTTATAAATTTAATTATCAACAAAAAAAAAAAAAAAACTAAAAATCAAATGTGTTATTAAATAACAAAAATTTAAAGATGAAATAAGTGTTTATAGTTTTAATTTTTAAAGATTAAAAATAAAAAACAAAAAAAATACTAAACAGGATCTTAATTTTTTGTTTTTAAAAATTAAACTTATTTACATTGTTTTCGCCTATTTTTTTATTTACTTTATAGAAAAGCTATCAAAATTAAAAATTACAAAATATATATTTAAAAATTATTCTAAATTTTTCTAACAAAAGTCAAAAACTAAATAGTTATTGAACAGATCTCTTCTCAATTGACTCTGATTCGAAACCAAACACGTTAATTGTAACAGGATGTTGCTTCTATGTTTGATGTTTTTGGTTTTTGGTTTTTTAGGGTTGTATAGGTACAGAGTGGGAGACGTTTTAAGAGTGGCAGGGTTCAAGAACAATGCACCACAATTCAACTTCATATGCAGAAAAAATGTTGCTTTAAGCATAGATTCAGACAAAACAGATGAAGTGGAATTACAAAATGCTGTCAAAAATGCACTAAACCATTTGATTCCATTTGATGTCACTTTGAGTGAGTACACAAGCTGTGTTGACACTTCAAAAATCCCAGGTCATTATGTGCTCTATTGGGAGCTAACTCTCAATAACAATGCAATTAAAACAACACCAATTCCACCCTCTGTGTTTGAAGATTGTTGCTTCACAATTGAAGAGTCTCTCAACGCCGTGTATCGTCAAGGTCGGGTCTCTGATAAGTCCATCGGTCCCCTTGAGATCAAGATCGTCGAAACCGGGACGTTCGATAAGCTCATGGACTATGCAATTAGCTTAGGAGCATCAATAAATCAGTATAAAACACCAAGATGTGTTAAGTATGAACCCATTATTCAACTATTGAATTCAAGGGTTGTGTCAAATTATTTCAGCCCTAAGTGCCCTAAATGGGTTCCAGGTCATAAGCAATGGAATTGAAATGGAGATTTTAATTGGATTATTGTATCATTATCAATAGGGAATATTTAAACACCCTAGAGATTTTATGGGTTTTTTTTTTTTTTTTTTTTTTTTCTAATTTTCTTCTTTTTGCATCCTTTCTGAAATTTTTAGCACCTCTTGGAAATGGGTTTAGGCTAATTTGAACTTAATAGACAATGTACAAAAGTAGTTCCTCTGTTCAAGAAAGTAGTGTTCCTTTTTCTTTCCCCCTTCCCTTTACTTATATGATCATTGAAG

mRNA sequence

CTATGCTTCTTTTTTTCCTCCTCACATCAAAAATCCCACCATCATTTCTTAAAAAAACCACACACAAACACACAATCTTCTCTTAATTACTCTTCTTTCCCTCTCTCTCTTTTTTTCAACTTTCCAAAAGACTATCCTTTTCCAACAACATGCCTGAAGAAACTCCTCAAAAGTCCTCATTTCTCAGCTCTACCACTGAATACTACAAACTGTTAGAGAAAAACAAGAAGACTCTTCAATTCATTGAAGATGTCACTGCCAACGCCGACCATGTCCAGCGTCGTGTCCTCACCGAGATCCTCACCCGCAATGCCGATGTCGAGTACCTCCGTCGACACGGCCTCGGTGGTGCCACCGACCGTAAGACCTTCAAAAAAACCCTTCCTCTCATCTCCTATGAGGATATTCAACCTGATATCAACCGTATTGCCAATGGGGACTCCTCTCCCATCCTCTGTTCCTCTCCCATTTCTGAGTTTCTCACCAGCTCGGGTACATCGGGAGGAGAGAGAAAGCTGATGCCAACAATAGAAGAAGAGCTAGAAAGAAGATCATCACTCTACAGCTTACTAATGCCAGTAATGAGCCAATTCATAGAAGGCTTAGAGAAAGGCAAAGGAATGTACTTTCTATTCATAAAATCAGAATCAAAAACCCCAAGTGGCTTACTAGCTCGCCCTGTTTTAACAAGCTATTACAAAAGCTCCCATTTCAAACACCGTCCATTTGACCCATACACAAACTACACAAGCCCAAACCAAGCCATCCTCTGTTCCGACTCTTACCAAAGCATGTACGCTCAGCTCCTCTGTGGCCTCCTCCACCGCACCGACGTCCTCCGTGTCGGCGCCGTCTTCGCCTCGGGCTTCATCCGAGCCATCCGGTTCTTGGAAAAGCACTTCCCACTCCTCTGCCACGACATTCGAACAGGGACTCTGAATTCAGAGATTACAGATCAGGGGATCCGTGAATCGTTGAGGAAAATTCTGAAACCGGACCCGAAACTGGCGGATTTTGTGGAAGGGGAATGTGGGAAGAAATGTTGGGGAGGAATTATTAGTAGGATATGGCCGAATACGAAGTATGTGGATGTGATTGTAACAGGGACAATGTCGCAGTACATAAAGACATTGGATTATTACAGTAATGGGTTGCCGATTGTTTGTACAATGTATGCTTCTTCGGAGTGTTATTTTGGAGTGAATTTGAAGCCGATGTGTAAACCAGAGGAAGTGGCGTATACGCTTATACCAACAATGGCGTATTTTGAGTTTCTTCCCGTGAATAGGAGTAAAGGGTTGAGTGATAAAGAGTTGCAGAAGGAGCTTGTGGATCTTGTTGATGTTCAGCTTGGTCAGGAGTATGAGCTCGTCGTTACCACCTATGCAGGGTTGTATAGGTACAGAGTGGGAGACGTTTTAAGAGTGGCAGGGTTCAAGAACAATGCACCACAATTCAACTTCATATGCAGAAAAAATGTTGCTTTAAGCATAGATTCAGACAAAACAGATGAAGTGGAATTACAAAATGCTGTCAAAAATGCACTAAACCATTTGATTCCATTTGATGTCACTTTGAGTGAGTACACAAGCTGTGTTGACACTTCAAAAATCCCAGGTCATTATGTGCTCTATTGGGAGCTAACTCTCAATAACAATGCAATTAAAACAACACCAATTCCACCCTCTGTGTTTGAAGATTGTTGCTTCACAATTGAAGAGTCTCTCAACGCCGTGTATCGTCAAGGTCGGGTCTCTGATAAGTCCATCGGTCCCCTTGAGATCAAGATCGTCGAAACCGGGACGTTCGATAAGCTCATGGACTATGCAATTAGCTTAGGAGCATCAATAAATCAGTATAAAACACCAAGATGTGTTAAGTATGAACCCATTATTCAACTATTGAATTCAAGGGTTGTGTCAAATTATTTCAGCCCTAAGTGCCCTAAATGGGTTCCAGGTCATAAGCAATGGAATTGAAATGGAGATTTTAATTGGATTATTGTATCATTATCAATAGGGAATATTTAAACACCCTAGAGATTTTATGGGTTTTTTTTTTTTTTTTTTTTTTTTCTAATTTTCTTCTTTTTGCATCCTTTCTGAAATTTTTAGCACCTCTTGGAAATGGGTTTAGGCTAATTTGAACTTAATAGACAATGTACAAAAGTAGTTCCTCTGTTCAAGAAAGTAGTGTTCCTTTTTCTTTCCCCCTTCCCTTTACTTATATGATCATTGAAG

Coding sequence (CDS)

ATGCCTGAAGAAACTCCTCAAAAGTCCTCATTTCTCAGCTCTACCACTGAATACTACAAACTGTTAGAGAAAAACAAGAAGACTCTTCAATTCATTGAAGATGTCACTGCCAACGCCGACCATGTCCAGCGTCGTGTCCTCACCGAGATCCTCACCCGCAATGCCGATGTCGAGTACCTCCGTCGACACGGCCTCGGTGGTGCCACCGACCGTAAGACCTTCAAAAAAACCCTTCCTCTCATCTCCTATGAGGATATTCAACCTGATATCAACCGTATTGCCAATGGGGACTCCTCTCCCATCCTCTGTTCCTCTCCCATTTCTGAGTTTCTCACCAGCTCGGGTACATCGGGAGGAGAGAGAAAGCTGATGCCAACAATAGAAGAAGAGCTAGAAAGAAGATCATCACTCTACAGCTTACTAATGCCAGTAATGAGCCAATTCATAGAAGGCTTAGAGAAAGGCAAAGGAATGTACTTTCTATTCATAAAATCAGAATCAAAAACCCCAAGTGGCTTACTAGCTCGCCCTGTTTTAACAAGCTATTACAAAAGCTCCCATTTCAAACACCGTCCATTTGACCCATACACAAACTACACAAGCCCAAACCAAGCCATCCTCTGTTCCGACTCTTACCAAAGCATGTACGCTCAGCTCCTCTGTGGCCTCCTCCACCGCACCGACGTCCTCCGTGTCGGCGCCGTCTTCGCCTCGGGCTTCATCCGAGCCATCCGGTTCTTGGAAAAGCACTTCCCACTCCTCTGCCACGACATTCGAACAGGGACTCTGAATTCAGAGATTACAGATCAGGGGATCCGTGAATCGTTGAGGAAAATTCTGAAACCGGACCCGAAACTGGCGGATTTTGTGGAAGGGGAATGTGGGAAGAAATGTTGGGGAGGAATTATTAGTAGGATATGGCCGAATACGAAGTATGTGGATGTGATTGTAACAGGGACAATGTCGCAGTACATAAAGACATTGGATTATTACAGTAATGGGTTGCCGATTGTTTGTACAATGTATGCTTCTTCGGAGTGTTATTTTGGAGTGAATTTGAAGCCGATGTGTAAACCAGAGGAAGTGGCGTATACGCTTATACCAACAATGGCGTATTTTGAGTTTCTTCCCGTGAATAGGAGTAAAGGGTTGAGTGATAAAGAGTTGCAGAAGGAGCTTGTGGATCTTGTTGATGTTCAGCTTGGTCAGGAGTATGAGCTCGTCGTTACCACCTATGCAGGGTTGTATAGGTACAGAGTGGGAGACGTTTTAAGAGTGGCAGGGTTCAAGAACAATGCACCACAATTCAACTTCATATGCAGAAAAAATGTTGCTTTAAGCATAGATTCAGACAAAACAGATGAAGTGGAATTACAAAATGCTGTCAAAAATGCACTAAACCATTTGATTCCATTTGATGTCACTTTGAGTGAGTACACAAGCTGTGTTGACACTTCAAAAATCCCAGGTCATTATGTGCTCTATTGGGAGCTAACTCTCAATAACAATGCAATTAAAACAACACCAATTCCACCCTCTGTGTTTGAAGATTGTTGCTTCACAATTGAAGAGTCTCTCAACGCCGTGTATCGTCAAGGTCGGGTCTCTGATAAGTCCATCGGTCCCCTTGAGATCAAGATCGTCGAAACCGGGACGTTCGATAAGCTCATGGACTATGCAATTAGCTTAGGAGCATCAATAAATCAGTATAAAACACCAAGATGTGTTAAGTATGAACCCATTATTCAACTATTGAATTCAAGGGTTGTGTCAAATTATTTCAGCCCTAAGTGCCCTAAATGGGTTCCAGGTCATAAGCAATGGAATTGA

Protein sequence

MPEETPQKSSFLSSTTEYYKLLEKNKKTLQFIEDVTANADHVQRRVLTEILTRNADVEYLRRHGLGGATDRKTFKKTLPLISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSSHFKHRPFDPYTNYTSPNQAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHFPLLCHDIRTGTLNSEITDQGIRESLRKILKPDPKLADFVEGECGKKCWGGIISRIWPNTKYVDVIVTGTMSQYIKTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVNRSKGLSDKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTSCVDTSKIPGHYVLYWELTLNNNAIKTTPIPPSVFEDCCFTIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHKQWN
Homology
BLAST of Bhi09G001660 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 1000.0 bits (2584), Expect = 8.4e-292
Identity = 485/598 (81.10%), Postives = 533/598 (89.13%), Query Frame = 0

Query: 21  LLEKNKKTLQFIEDVTANADHVQRRVLTEILTRNADVEYLRRHGLGGATDRKTFKKTLPL 80
           L EKNK  LQFIEDVT NAD VQRRVL EIL+RNADVEYL+RHGL G TDR+TFK  +P+
Sbjct: 17  LAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPV 76

Query: 81  ISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSL 140
           ++YEDIQP+INRIANGD S +LCS+PISEFLTSSGTSGGERKLMPTIEEEL+RRS LYSL
Sbjct: 77  VTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 141 LMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSSHFKHRPFDPYTNYT 200
           LMPVM QF+ GL+KGKGMYFLFIKSESKTP GL ARPVLTSYYKSSHFK+RP+DPYTNYT
Sbjct: 137 LMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYT 196

Query: 201 SPNQAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHFPLLCHDIRT 260
           SPNQ ILCSDSYQSMY+Q+LCGL    +VLRVGAVFASGFIRAI+FLEKH+P L  DIRT
Sbjct: 197 SPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRT 256

Query: 261 GTLNSEITDQGIRESLRKILKPDPKLADFVEGECGKKCWGGIISRIWPNTKYVDVIVTGT 320
           GTL+SEITD  +RE++ +ILKPDPKLADFVE EC K  W GII+R+WPNTKYVDVIVTGT
Sbjct: 257 GTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGT 316

Query: 321 MSQYIKTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVNR 380
           MSQYI TLDYYSNGLP+VCTMYASSECYFGVNL+P+CKP EV+YTLIP MAYFEFLPV+R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHR 376

Query: 381 SKG----------LSDKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFK 440
           + G          L++KE Q+ELVDLVDV+LGQEYELVVTTYAGLYRYRVGDVL VAGFK
Sbjct: 377 NSGVTSSISLPKALTEKE-QQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFK 436

Query: 441 NNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTSCVDTSKIPG 500
           NNAPQF+FICRKNV LSIDSDKTDEVELQNAVKNA+ HL+PFD +LSEYTS  DTS IPG
Sbjct: 437 NNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 496

Query: 501 HYVLYWELTLNNNAIKTTPIPPSVFEDCCFTIEESLNAVYRQGRVSDKSIGPLEIKIVET 560
           HYVL+WEL LN N    TPIPPSVFEDCC TIEESLN+VYRQGRVSDKSIGPLEIK+VE+
Sbjct: 497 HYVLFWELCLNGN----TPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVES 556

Query: 561 GTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHKQW 609
           GTFDKLMDYAISLGASINQYKTPRCVK+ PII+LLNSRVV +YFSPKCPKW PGHKQW
Sbjct: 557 GTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQW 609

BLAST of Bhi09G001660 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 972.6 bits (2513), Expect = 1.4e-283
Identity = 480/618 (77.67%), Postives = 532/618 (86.08%), Query Frame = 0

Query: 1   MPEETPQKSSFLSSTTEYYKLLEKNKKTLQFIEDVTANADHVQRRVLTEILTRNADVEYL 60
           MPE   ++S  +   T    L +KNK+ LQ IE++T+NAD VQR+VL EILTRNADVEYL
Sbjct: 1   MPEAPKKESLEVFDLT----LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYL 60

Query: 61  RRHGLGGATDRKTFKKTLPLISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGE 120
           RRH L G TDR+TFK  +P+I+YEDI+P+INRIANGD SPIL S PISEFLTSSGTSGGE
Sbjct: 61  RRHDLNGRTDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGE 120

Query: 121 RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT 180
           RKLMPTIEEEL+RRS LYSLLMPVMSQF+ GLE GKGMYFLFIKSESKTP GL ARPVLT
Sbjct: 121 RKLMPTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLT 180

Query: 181 SYYKSSHFKHRPFDPYTNYTSPNQAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF 240
           SYYKSSHFK RP+DPYTNYTSPN+ ILCSDSYQSMY+Q+LCGL    +VLRVGAVFASGF
Sbjct: 181 SYYKSSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGF 240

Query: 241 IRAIRFLEKHFPLLCHDIRTGTLNSEITDQGIRESLRKILKPDPKLADFVEGECGKKCWG 300
           IRAI+FLEKH+  L  DIRTGTL+S ITD  +RE++ KILKP PKLADFVE EC K  W 
Sbjct: 241 IRAIKFLEKHWIELVRDIRTGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQ 300

Query: 301 GIISRIWPNTKYVDVIVTGTMSQYIKTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPE 360
           GII+R+WPNTKYVDVIVTGTMSQYI TLDYYSNGLP+VCTMYASSECYFGVNL+P+CKP 
Sbjct: 301 GIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPS 360

Query: 361 EVAYTLIPTMAYFEFLPVNRSKG----------LSDKELQKELVDLVDVQLGQEYELVVT 420
           EV+YTLIP+MAYFEFLPV+R+ G          L++KE Q+ELVDLVDV+LGQEYELVVT
Sbjct: 361 EVSYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKE-QQELVDLVDVKLGQEYELVVT 420

Query: 421 TYAGLYRYRVGDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLI 480
           TYAGL RYRVGD+LRV GFKN APQF+FICRKNV LSIDSDKTDEVELQNAVKNA+ HL+
Sbjct: 421 TYAGLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLV 480

Query: 481 PFDVTLSEYTSCVDTSKIPGHYVLYWELTLNNNAIKTTPIPPSVFEDCCFTIEESLNAVY 540
           PFD +LSEYTS  DTS IPGHYVL+WEL L+ N    TPIPPSVFEDCC  +EES N VY
Sbjct: 481 PFDASLSEYTSYADTSSIPGHYVLFWELCLDGN----TPIPPSVFEDCCLAVEESFNTVY 540

Query: 541 RQGRVSDKSIGPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVV 600
           RQGRVSDKSIGPLEIKIVE GTFDKLMDYAISLGASINQYKTPRCVK+ PII+LLNSRVV
Sbjct: 541 RQGRVSDKSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVV 600

Query: 601 SNYFSPKCPKWVPGHKQW 609
            +YFSPKCPKWVPGHKQW
Sbjct: 601 DSYFSPKCPKWVPGHKQW 609

BLAST of Bhi09G001660 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 777.3 bits (2006), Expect = 8.9e-225
Identity = 381/586 (65.02%), Postives = 456/586 (77.82%), Query Frame = 0

Query: 23  EKNKKTLQFIEDVTANADHVQRRVLTEILTRNADVEYLRRHGLGGATDRKTFKKTLPLIS 82
           EK+ K L+FIE++T NAD VQ  +L EIL RNAD EYLRR  L GATDR TFK  +P+I+
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 83  YEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSLLM 142
           YED+QP+I RIA+GD SPIL + PISEFLTSSGTS GERKLMPTI+EEL+RR  LYSLLM
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 143 PVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSSHFKHRPFDPYTNYTSP 202
           PVM+ ++ GL+KGKGMYFLF+KSE+KTP GL ARPVLTSYYKS HF+ RP+DPY  YTSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196

Query: 203 NQAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHFPLLCHDIRTGT 262
           N+AILC DS+QSMY Q+LCGLL R  VLRVGAVFASG +RAIRFL+ H+    HDI  G 
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256

Query: 263 LNSEITDQGIRESLRKILKPDPKLADFVEGECGKKCWGGIISRIWPNTKYVDVIVTGTMS 322
           L+SEITD  IR+ +  ILKPDP LA+F+  EC    W  II+RIWPNTKY+DVIVTG M+
Sbjct: 257 LDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMA 316

Query: 323 QYIKTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVNRSK 382
           QYI TL+YYS GLP+ CTMYASSECYFG+NL PM KP EV+YT++P MAYFEF+P+  +K
Sbjct: 317 QYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGTK 376

Query: 383 GLSDKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQFNFICRK 442
                      V+LVDV +G+EYELVVTTYAGL RYRVGD+LRV GF N+APQF+F+ RK
Sbjct: 377 A----------VELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRK 436

Query: 443 NVALSIDSDKTDEVELQNAVKNALNHL-IPFDVTLSEYTSCVDTSKIPGHYVLYWELTLN 502
           NV LSIDSDKTDE ELQ AV+NA + L       ++EYTS  DTS IPGHYVLYWEL + 
Sbjct: 437 NVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVR 496

Query: 503 NNAIKTTPIPPSVFEDCCFTIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLMDYAI 562
           + A + +         CC  +EESLN+VYRQ RV+D S+GPLEI++V  GTF++LMDYAI
Sbjct: 497 DGARQPS---HETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAI 556

Query: 563 SLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHKQ 608
           S GASINQYK PRCV + PI++LL+SRVVS +FSP  P W P  ++
Sbjct: 557 SRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRR 589

BLAST of Bhi09G001660 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 770.4 bits (1988), Expect = 1.1e-222
Identity = 380/610 (62.30%), Postives = 471/610 (77.21%), Query Frame = 0

Query: 1   MPEETPQKSSFLSSTTEYYKLLEKNKKTLQFIEDVTANADHVQRRVLTEILTRNADVEYL 60
           M  ++P +S  +S+TT      EK+ K L+FIE++T N D VQ +VL EILTRN++ EYL
Sbjct: 1   MAVDSPLQSRMVSATTS-----EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYL 60

Query: 61  RRHGLGGATDRKTFKKTLPLISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGE 120
           +R  L G  DRKTFK  +P+++YED++P+I RI+NGD SPIL S PI+EFLTSSGTS GE
Sbjct: 61  KRFDLDGVVDRKTFKSKVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGE 120

Query: 121 RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT 180
           RKLMPTIEE+L+RR  LYSLLMPVM+ ++ GL+KGKG+YFLF+KSESKT  GL ARPVLT
Sbjct: 121 RKLMPTIEEDLDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLT 180

Query: 181 SYYKSSHFKHRPFDPYTNYTSPNQAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF 240
           SYYKS HFK RP+DPY  YTSPN+AILCSDS QSMYAQ+LCGLL R +VLR+GAVFASG 
Sbjct: 181 SYYKSDHFKRRPYDPYNVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGL 240

Query: 241 IRAIRFLEKHFPLLCHDIRTGTLNSEITDQGIRESLRKIL-KPDPKLADFVEGECGKKCW 300
           +RAI FL+ ++  L  DI TGTL+S I D  I+  + KIL KPD +LA+F+ G C ++ W
Sbjct: 241 LRAISFLQNNWKELARDISTGTLSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENW 300

Query: 301 GGIISRIWPNTKYVDVIVTGTMSQYIKTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKP 360
            GII++IWPNTKY+DVIVTG M+QYI TL+YYS GLP+ CTMYASSE YFG+NLKPMCKP
Sbjct: 301 EGIITKIWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKP 360

Query: 361 EEVAYTLIPTMAYFEFLPVNR-SKGLSDKEL-QKELVDLVDVQLGQEYELVVTTYAGLYR 420
            EV+YT++P MAYFEFLP N    G ++  L +  LV+L +V++G+EYELV+TTYAGLYR
Sbjct: 361 SEVSYTIMPNMAYFEFLPHNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYR 420

Query: 421 YRVGDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLS 480
           YRVGD+LRV GF N+APQF FI RKNV LS++SDKTDE ELQ AV+NA          + 
Sbjct: 421 YRVGDILRVTGFHNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVI 480

Query: 481 EYTSCVDTSKIPGHYVLYWELTLNNNAIKTTPIPPSVFEDCCFTIEESLNAVYRQGRVSD 540
           EYTS  +T  IPGHYV+YWEL   + +     +   V   CC  +EESLN+VYRQ RV+D
Sbjct: 481 EYTSYAETKTIPGHYVIYWELLGRDQS--NALMSEEVMAKCCLEMEESLNSVYRQSRVAD 540

Query: 541 KSIGPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPK 600
           KSIGPLEI++V  GTF++LMDYAIS GASINQYK PRCV + PI++LL+SRVVS +FSP 
Sbjct: 541 KSIGPLEIRVVRNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPS 600

Query: 601 CPKWVPGHKQ 608
            P W P  ++
Sbjct: 601 LPHWSPERRR 603

BLAST of Bhi09G001660 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 751.1 bits (1938), Expect = 6.8e-217
Identity = 369/586 (62.97%), Postives = 458/586 (78.16%), Query Frame = 0

Query: 24  KNKKTLQFIEDVTANADHVQRRVLTEILTRNADVEYLRRHGLGGATDRKTFKKTLPLISY 83
           K+ K L+FIE++T N D VQ++V+ EIL+RN+D EYL+R GL G TDRKTFK  +P++ Y
Sbjct: 18  KDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPVVIY 77

Query: 84  EDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSLLMP 143
           +D++P+I RIANGD S IL S PI+EFLTSSGTS GERKLMPTI+E+++RR  LYSLLMP
Sbjct: 78  DDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSLLMP 137

Query: 144 VMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSSHFKHRPFDPYTNYTSPN 203
           VM+ ++ GL+KGK +YFLF+K+ESKTP GL ARPVLTSYYKS  FK RP DPY  YTSPN
Sbjct: 138 VMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNVYTSPN 197

Query: 204 QAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHFPLLCHDIRTGTL 263
           +AILC DS QSMY Q+LCGLL R +VLR+GAVFASG +RAI FL+ ++  L  DI TGTL
Sbjct: 198 EAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDISTGTL 257

Query: 264 NSEITDQGIRESLRKIL-KPDPKLADFVEGECGK-KCWGGIISRIWPNTKYVDVIVTGTM 323
           +S I+D  I+ES+ KIL KPD +LADF+   CG+   W GII++IWPNTKY+DVIVTG M
Sbjct: 258 SSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVTGAM 317

Query: 324 SQYIKTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVNRS 383
           +QYI  L+YYS GLP+ CTMYASSE YFG+NLKPMCKP EV+YT++P MAYFEFLP +  
Sbjct: 318 AQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHHEV 377

Query: 384 KGLSDKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQFNFICR 443
                   + ELV+L DV++G+EYELV+TTYAGL RYRVGD+L+V GF N+APQF F+ R
Sbjct: 378 -----PTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFKFVRR 437

Query: 444 KNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTSCVDTSKIPGHYVLYWELTLN 503
           KNV LSI+SDKTDE ELQ+AV+NA   L      + EYTS  +T  IPGHYV+YWEL + 
Sbjct: 438 KNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWELLVK 497

Query: 504 NNAIKTTPIPPSVFEDCCFTIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLMDYAI 563
           +   +T P    V   CC  +EESLN+VYRQ RV+DKSIGPLEI++V+ GTF++LMDYAI
Sbjct: 498 D---QTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMDYAI 557

Query: 564 SLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHKQ 608
           S GASINQYK PRCV + PI++LL+SRVVS +FSP  P W P  ++
Sbjct: 558 SRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERRR 595

BLAST of Bhi09G001660 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 1000.0 bits (2584), Expect = 1.2e-290
Identity = 485/598 (81.10%), Postives = 533/598 (89.13%), Query Frame = 0

Query: 21  LLEKNKKTLQFIEDVTANADHVQRRVLTEILTRNADVEYLRRHGLGGATDRKTFKKTLPL 80
           L EKNK  LQFIEDVT NAD VQRRVL EIL+RNADVEYL+RHGL G TDR+TFK  +P+
Sbjct: 17  LAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPV 76

Query: 81  ISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSL 140
           ++YEDIQP+INRIANGD S +LCS+PISEFLTSSGTSGGERKLMPTIEEEL+RRS LYSL
Sbjct: 77  VTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 141 LMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSSHFKHRPFDPYTNYT 200
           LMPVM QF+ GL+KGKGMYFLFIKSESKTP GL ARPVLTSYYKSSHFK+RP+DPYTNYT
Sbjct: 137 LMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYT 196

Query: 201 SPNQAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHFPLLCHDIRT 260
           SPNQ ILCSDSYQSMY+Q+LCGL    +VLRVGAVFASGFIRAI+FLEKH+P L  DIRT
Sbjct: 197 SPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRT 256

Query: 261 GTLNSEITDQGIRESLRKILKPDPKLADFVEGECGKKCWGGIISRIWPNTKYVDVIVTGT 320
           GTL+SEITD  +RE++ +ILKPDPKLADFVE EC K  W GII+R+WPNTKYVDVIVTGT
Sbjct: 257 GTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGT 316

Query: 321 MSQYIKTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVNR 380
           MSQYI TLDYYSNGLP+VCTMYASSECYFGVNL+P+CKP EV+YTLIP MAYFEFLPV+R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHR 376

Query: 381 SKG----------LSDKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFK 440
           + G          L++KE Q+ELVDLVDV+LGQEYELVVTTYAGLYRYRVGDVL VAGFK
Sbjct: 377 NSGVTSSISLPKALTEKE-QQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFK 436

Query: 441 NNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTSCVDTSKIPG 500
           NNAPQF+FICRKNV LSIDSDKTDEVELQNAVKNA+ HL+PFD +LSEYTS  DTS IPG
Sbjct: 437 NNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 496

Query: 501 HYVLYWELTLNNNAIKTTPIPPSVFEDCCFTIEESLNAVYRQGRVSDKSIGPLEIKIVET 560
           HYVL+WEL LN N    TPIPPSVFEDCC TIEESLN+VYRQGRVSDKSIGPLEIK+VE+
Sbjct: 497 HYVLFWELCLNGN----TPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVES 556

Query: 561 GTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHKQW 609
           GTFDKLMDYAISLGASINQYKTPRCVK+ PII+LLNSRVV +YFSPKCPKW PGHKQW
Sbjct: 557 GTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQW 609

BLAST of Bhi09G001660 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 972.6 bits (2513), Expect = 2.0e-282
Identity = 480/618 (77.67%), Postives = 532/618 (86.08%), Query Frame = 0

Query: 1   MPEETPQKSSFLSSTTEYYKLLEKNKKTLQFIEDVTANADHVQRRVLTEILTRNADVEYL 60
           MPE   ++S  +   T    L +KNK+ LQ IE++T+NAD VQR+VL EILTRNADVEYL
Sbjct: 1   MPEAPKKESLEVFDLT----LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYL 60

Query: 61  RRHGLGGATDRKTFKKTLPLISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGE 120
           RRH L G TDR+TFK  +P+I+YEDI+P+INRIANGD SPIL S PISEFLTSSGTSGGE
Sbjct: 61  RRHDLNGRTDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGE 120

Query: 121 RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT 180
           RKLMPTIEEEL+RRS LYSLLMPVMSQF+ GLE GKGMYFLFIKSESKTP GL ARPVLT
Sbjct: 121 RKLMPTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLT 180

Query: 181 SYYKSSHFKHRPFDPYTNYTSPNQAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF 240
           SYYKSSHFK RP+DPYTNYTSPN+ ILCSDSYQSMY+Q+LCGL    +VLRVGAVFASGF
Sbjct: 181 SYYKSSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGF 240

Query: 241 IRAIRFLEKHFPLLCHDIRTGTLNSEITDQGIRESLRKILKPDPKLADFVEGECGKKCWG 300
           IRAI+FLEKH+  L  DIRTGTL+S ITD  +RE++ KILKP PKLADFVE EC K  W 
Sbjct: 241 IRAIKFLEKHWIELVRDIRTGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQ 300

Query: 301 GIISRIWPNTKYVDVIVTGTMSQYIKTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPE 360
           GII+R+WPNTKYVDVIVTGTMSQYI TLDYYSNGLP+VCTMYASSECYFGVNL+P+CKP 
Sbjct: 301 GIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPS 360

Query: 361 EVAYTLIPTMAYFEFLPVNRSKG----------LSDKELQKELVDLVDVQLGQEYELVVT 420
           EV+YTLIP+MAYFEFLPV+R+ G          L++KE Q+ELVDLVDV+LGQEYELVVT
Sbjct: 361 EVSYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKE-QQELVDLVDVKLGQEYELVVT 420

Query: 421 TYAGLYRYRVGDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLI 480
           TYAGL RYRVGD+LRV GFKN APQF+FICRKNV LSIDSDKTDEVELQNAVKNA+ HL+
Sbjct: 421 TYAGLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLV 480

Query: 481 PFDVTLSEYTSCVDTSKIPGHYVLYWELTLNNNAIKTTPIPPSVFEDCCFTIEESLNAVY 540
           PFD +LSEYTS  DTS IPGHYVL+WEL L+ N    TPIPPSVFEDCC  +EES N VY
Sbjct: 481 PFDASLSEYTSYADTSSIPGHYVLFWELCLDGN----TPIPPSVFEDCCLAVEESFNTVY 540

Query: 541 RQGRVSDKSIGPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVV 600
           RQGRVSDKSIGPLEIKIVE GTFDKLMDYAISLGASINQYKTPRCVK+ PII+LLNSRVV
Sbjct: 541 RQGRVSDKSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVV 600

Query: 601 SNYFSPKCPKWVPGHKQW 609
            +YFSPKCPKWVPGHKQW
Sbjct: 601 DSYFSPKCPKWVPGHKQW 609

BLAST of Bhi09G001660 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 1.5e-240
Identity = 396/584 (67.81%), Postives = 474/584 (81.16%), Query Frame = 0

Query: 27  KTLQFIEDVTANADHVQRRVLTEILTRNADVEYLRRHGLGGATD-RKTFKKTLPLISYED 86
           + L+FIE VTANA  VQRRVL EIL +NA  EYLRR+G+ G+ D    F++ +PL++YE 
Sbjct: 22  EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEG 81

Query: 87  IQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSLLMPVM 146
           +QPDI RIANGD+SPI    PISEFLTSSGTSGGERKLMPTI +E+ RRS LYSLLMPVM
Sbjct: 82  LQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVM 141

Query: 147 SQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSSHFKHRPFDPYTNYTSPNQA 206
           SQ + GL+KGK MY LF+K+ES+TP GL ARPVLTSYY+S  F  RP DPYT+YTSP++A
Sbjct: 142 SQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEA 201

Query: 207 ILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHFPLLCHDIRTGTLNS 266
           ILC DSYQSMYAQLLCGL+HR DVLRVGAVFASGF+RAI FLEKH+  LCHDIRTG L+ 
Sbjct: 202 ILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDP 261

Query: 267 EITDQGIRESLRKILKPDPKLADFVEGECGKKCWGGIISRIWPNTKYVDVIVTGTMSQYI 326
           EITD+ +R+++ ++L+ DP LAD +E EC +  W GII R+WP TKY+DVIVTGTMSQYI
Sbjct: 262 EITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYI 321

Query: 327 KTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVNRSKGLS 386
            TL++Y  GLP+ CTMYASSECYFG+NL PMCKP +VAYTLIPTM Y+EFLPVN +   +
Sbjct: 322 PTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATA 381

Query: 387 DKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQFNFICRKNVA 446
           +    ++LVDLVDV+LG EYELVVTTY+GLYRYRVGDVLRVAGFKN AP F+F+ R+NVA
Sbjct: 382 EAS-HRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQNVA 441

Query: 447 LSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTSCVDTSKIPGHYVLYWELTLNNNAI 506
           LS+DSDKTDE EL  AV  A+ HL PF  +L EYTS  D + IPGHYVL+WEL       
Sbjct: 442 LSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWELRAG---- 501

Query: 507 KTTPIPPSVFEDCCFTIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLMDYAISLGA 566
            +T +P SVFE+CC ++EE+LN+VYRQGR  D+SIGPLEI++V  GTFDKLMDYAIS GA
Sbjct: 502 -STAVPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGA 561

Query: 567 SINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHKQWN 610
           SINQYK PRCV+  P+++LL++RV   YFSPKCPKW PG+KQWN
Sbjct: 562 SINQYKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSPGNKQWN 599

BLAST of Bhi09G001660 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 4.4e-229
Identity = 388/592 (65.54%), Postives = 464/592 (78.38%), Query Frame = 0

Query: 24  KNKKTLQFIEDVTANADHVQRRVLTEILTRNADVEYLRRHGLGGATD--RKTFKKTLPLI 83
           ++++ L+ IE VT NA   QRRVL EIL +NA  EYLRR G+ G      + F++  PL+
Sbjct: 22  EHREKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYLRRLGVPGDAPGADEAFRRLAPLV 81

Query: 84  SYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSLL 143
           +YEDI PD+ RIANGD+SPIL   P+SEFLTSSGTSGGERKLMPTIEEE+ERRS LYSLL
Sbjct: 82  TYEDILPDVLRIANGDTSPILSGKPVSEFLTSSGTSGGERKLMPTIEEEMERRSGLYSLL 141

Query: 144 MPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSSHFKHRPFDPYTNYTS 203
           MPVMS+ + GL+KGK MY  F+KSE +TP GL ARPVLTS+Y+S +F  RP DPYT YTS
Sbjct: 142 MPVMSRQVPGLDKGKAMYLYFVKSEWRTPGGLPARPVLTSFYRSRYFLERPHDPYTVYTS 201

Query: 204 PNQAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHFPLLCHDIRTG 263
           P++A+LC D+YQSMYAQL+CGL+HR DVLRVGAVFASGF+RAIRFLEKH+P LC DIR G
Sbjct: 202 PDEAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFASGFLRAIRFLEKHWPSLCRDIRAG 261

Query: 264 TLNSEITDQGIRESLRKILK-PDPKLADFVEGECGKKCWGGIISRIWPNTKYVDVIVTGT 323
            L+  +TD  +R ++ ++L+  DP LAD +E EC +  W GII R+WP+TKY+DVIVTG 
Sbjct: 262 ELDGGVTDPAVRGAVGRVLRGADPALADAIEAECARPSWQGIIRRVWPSTKYIDVIVTGA 321

Query: 324 MSQYIKTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVNR 383
           M+QYI TL++Y  GLP+ CTMYASSECYFG+NL PMCKP EVAYTLIPTM YFEFLPVN 
Sbjct: 322 MAQYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCKPSEVAYTLIPTMCYFEFLPVNS 381

Query: 384 SKG--LSDKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQFNF 443
                 + +   + LVDLVDV+LG EYELVVTTY+GLYRYRVGDVLRVAGFKN AP F F
Sbjct: 382 GANDVAAPEPDHRGLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNAAPMFAF 441

Query: 444 ICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYTSCVDT-SKIPGHYVLYWE 503
           + RKNVALSIDSDKTDE EL  AV  A+ HL PF  +L EYTS  DT + IPGHYVL+WE
Sbjct: 442 VRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEYTSYADTATTIPGHYVLFWE 501

Query: 504 LTLNNNAIKTTPIPPSVFEDCCFTIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLM 563
           L    +    TP+P SVFEDCC  +EE LN+VYRQ R +D+SIGPLEI++V  GTFDKLM
Sbjct: 502 L---RSPAGGTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIGPLEIRVVADGTFDKLM 561

Query: 564 DYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW-VPGHKQW 609
           DYA+S GASINQYK PRCV+  P+++LL+ RV + YFSPKCPKW   G+KQW
Sbjct: 562 DYALSRGASINQYKAPRCVRPGPVVELLDGRVQATYFSPKCPKWCAGGNKQW 610

BLAST of Bhi09G001660 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 1.3e-223
Identity = 381/586 (65.02%), Postives = 456/586 (77.82%), Query Frame = 0

Query: 23  EKNKKTLQFIEDVTANADHVQRRVLTEILTRNADVEYLRRHGLGGATDRKTFKKTLPLIS 82
           EK+ K L+FIE++T NAD VQ  +L EIL RNAD EYLRR  L GATDR TFK  +P+I+
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 83  YEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSSLYSLLM 142
           YED+QP+I RIA+GD SPIL + PISEFLTSSGTS GERKLMPTI+EEL+RR  LYSLLM
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 143 PVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTSYYKSSHFKHRPFDPYTNYTSP 202
           PVM+ ++ GL+KGKGMYFLF+KSE+KTP GL ARPVLTSYYKS HF+ RP+DPY  YTSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196

Query: 203 NQAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFIRAIRFLEKHFPLLCHDIRTGT 262
           N+AILC DS+QSMY Q+LCGLL R  VLRVGAVFASG +RAIRFL+ H+    HDI  G 
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256

Query: 263 LNSEITDQGIRESLRKILKPDPKLADFVEGECGKKCWGGIISRIWPNTKYVDVIVTGTMS 322
           L+SEITD  IR+ +  ILKPDP LA+F+  EC    W  II+RIWPNTKY+DVIVTG M+
Sbjct: 257 LDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMA 316

Query: 323 QYIKTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPEEVAYTLIPTMAYFEFLPVNRSK 382
           QYI TL+YYS GLP+ CTMYASSECYFG+NL PM KP EV+YT++P MAYFEF+P+  +K
Sbjct: 317 QYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGTK 376

Query: 383 GLSDKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQFNFICRK 442
                      V+LVDV +G+EYELVVTTYAGL RYRVGD+LRV GF N+APQF+F+ RK
Sbjct: 377 A----------VELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRK 436

Query: 443 NVALSIDSDKTDEVELQNAVKNALNHL-IPFDVTLSEYTSCVDTSKIPGHYVLYWELTLN 502
           NV LSIDSDKTDE ELQ AV+NA + L       ++EYTS  DTS IPGHYVLYWEL + 
Sbjct: 437 NVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVR 496

Query: 503 NNAIKTTPIPPSVFEDCCFTIEESLNAVYRQGRVSDKSIGPLEIKIVETGTFDKLMDYAI 562
           + A + +         CC  +EESLN+VYRQ RV+D S+GPLEI++V  GTF++LMDYAI
Sbjct: 497 DGARQPS---HETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAI 556

Query: 563 SLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHKQ 608
           S GASINQYK PRCV + PI++LL+SRVVS +FSP  P W P  ++
Sbjct: 557 SRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRR 589

BLAST of Bhi09G001660 vs. ExPASy TrEMBL
Match: A0A5A7V8A5 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold977G00060 PE=3 SV=1)

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 572/608 (94.08%), Postives = 584/608 (96.05%), Query Frame = 0

Query: 1   MPEETPQKSSFLSSTTEYYKLLEKNKKTLQFIEDVTANADHVQRRVLTEILTRNADVEYL 60
           MPEETPQKSSFL STTEYYKLLEKNKKTLQFIEDVT NAD VQRRVLTEIL+RNA VEYL
Sbjct: 1   MPEETPQKSSFL-STTEYYKLLEKNKKTLQFIEDVTTNADQVQRRVLTEILSRNAGVEYL 60

Query: 61  RRHGLGGATDRKTFKKTLPLISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGE 120
           RRHGLGGATD KTFK TLPLISYEDIQPDINRIANGD+SPILCSSPISEFLTSSGTSGGE
Sbjct: 61  RRHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDTSPILCSSPISEFLTSSGTSGGE 120

Query: 121 RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT 180
           RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT
Sbjct: 121 RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT 180

Query: 181 SYYKSSHFKHRPFDPYTNYTSPNQAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF 240
           SYYKS HFK+RPFDPYTNYTSPN+AILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF
Sbjct: 181 SYYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF 240

Query: 241 IRAIRFLEKHFPLLCHDIRTGTLNSEITDQGIRESLRKILKPDPKLADFVEGECGKKCWG 300
           IRAIRFLEKH+ LLCHDIRTGTLNSEITDQGIRES+RKILK D KLADFVEGECGKKCWG
Sbjct: 241 IRAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWG 300

Query: 301 GIISRIWPNTKYVDVIVTGTMSQYIKTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPE 360
           GIISRIWPNTKYVDVIVTGTMSQYI TLDYYSNGLPIVCTMYASSECYFGVNLKPMCKP 
Sbjct: 301 GIISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPG 360

Query: 361 EVAYTLIPTMAYFEFLPVNRSKGLSDKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRV 420
           EVAYTLIPTMAYFEFLPVNRSK   +KEL+KELVDLVDV+LG EYELVVTTY+GLYRYRV
Sbjct: 361 EVAYTLIPTMAYFEFLPVNRSK---EKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRV 420

Query: 421 GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYT 480
           GD+LRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL HLIPFDVTLSEYT
Sbjct: 421 GDILRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYT 480

Query: 481 SCVDTSKIPGHYVLYWELTLNNNAIKTTPIPPSVFEDCCFTIEESLNAVYRQGRVSDKSI 540
           SCVDTSKIPGHYVLYWELT  NN IK+TPIPPSVFEDCCF IEESLNAVYRQGRVSDKSI
Sbjct: 481 SCVDTSKIPGHYVLYWELT-PNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSI 540

Query: 541 GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPK 600
           GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSR VSNYFSPKCPK
Sbjct: 541 GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRTVSNYFSPKCPK 600

Query: 601 WVPGHKQW 609
           WVP  KQW
Sbjct: 601 WVP--KQW 601

BLAST of Bhi09G001660 vs. ExPASy TrEMBL
Match: A0A1S3BYK9 (indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103494980 PE=3 SV=1)

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 572/608 (94.08%), Postives = 584/608 (96.05%), Query Frame = 0

Query: 1   MPEETPQKSSFLSSTTEYYKLLEKNKKTLQFIEDVTANADHVQRRVLTEILTRNADVEYL 60
           MPEETPQKSSFL STTEYYKLLEKNKKTLQFIEDVT NAD VQRRVLTEIL+RNA VEYL
Sbjct: 1   MPEETPQKSSFL-STTEYYKLLEKNKKTLQFIEDVTTNADQVQRRVLTEILSRNAGVEYL 60

Query: 61  RRHGLGGATDRKTFKKTLPLISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGE 120
           RRHGLGGATD KTFK TLPLISYEDIQPDINRIANGD+SPILCSSPISEFLTSSGTSGGE
Sbjct: 61  RRHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDTSPILCSSPISEFLTSSGTSGGE 120

Query: 121 RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT 180
           RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT
Sbjct: 121 RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT 180

Query: 181 SYYKSSHFKHRPFDPYTNYTSPNQAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF 240
           SYYKS HFK+RPFDPYTNYTSPN+AILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF
Sbjct: 181 SYYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF 240

Query: 241 IRAIRFLEKHFPLLCHDIRTGTLNSEITDQGIRESLRKILKPDPKLADFVEGECGKKCWG 300
           IRAIRFLEKH+ LLCHDIRTGTLNSEITDQGIRES+RKILK D KLADFVEGECGKKCWG
Sbjct: 241 IRAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWG 300

Query: 301 GIISRIWPNTKYVDVIVTGTMSQYIKTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPE 360
           GIISRIWPNTKYVDVIVTGTMSQYI TLDYYSNGLPIVCTMYASSECYFGVNLKPMCKP 
Sbjct: 301 GIISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPG 360

Query: 361 EVAYTLIPTMAYFEFLPVNRSKGLSDKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRV 420
           EVAYTLIPTMAYFEFLPVNRSK   +KEL+KELVDLVDV+LG EYELVVTTY+GLYRYRV
Sbjct: 361 EVAYTLIPTMAYFEFLPVNRSK---EKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRV 420

Query: 421 GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYT 480
           GD+LRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL HLIPFDVTLSEYT
Sbjct: 421 GDILRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYT 480

Query: 481 SCVDTSKIPGHYVLYWELTLNNNAIKTTPIPPSVFEDCCFTIEESLNAVYRQGRVSDKSI 540
           SCVDTSKIPGHYVLYWELT  NN IK+TPIPPSVFEDCCF IEESLNAVYRQGRVSDKSI
Sbjct: 481 SCVDTSKIPGHYVLYWELT-PNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSI 540

Query: 541 GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPK 600
           GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSR VSNYFSPKCPK
Sbjct: 541 GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRTVSNYFSPKCPK 600

Query: 601 WVPGHKQW 609
           WVP  KQW
Sbjct: 601 WVP--KQW 601

BLAST of Bhi09G001660 vs. ExPASy TrEMBL
Match: A0A0A0LAR6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G431430 PE=3 SV=1)

HSP 1 Score: 1146.0 bits (2963), Expect = 0.0e+00
Identity = 571/608 (93.91%), Postives = 584/608 (96.05%), Query Frame = 0

Query: 1   MPEETPQKSSFLSSTTEYYKLLEKNKKTLQFIEDVTANADHVQRRVLTEILTRNADVEYL 60
           MPEETPQ SSFL STTEYYKLLEKNKKTLQFIEDVTANAD VQRRVLTEIL+RNA VEYL
Sbjct: 1   MPEETPQNSSFL-STTEYYKLLEKNKKTLQFIEDVTANADQVQRRVLTEILSRNAGVEYL 60

Query: 61  RRHGLGGATDRKTFKKTLPLISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGE 120
           RRHGLGGATD KTFK TLPLISYEDIQPDINRIANGDSS ILCSSPISEFLTSSGTSGGE
Sbjct: 61  RRHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDSSSILCSSPISEFLTSSGTSGGE 120

Query: 121 RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT 180
           RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT
Sbjct: 121 RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT 180

Query: 181 SYYKSSHFKHRPFDPYTNYTSPNQAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF 240
           SYYKS HFK+RPFDPYTNYTSPN+AILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF
Sbjct: 181 SYYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF 240

Query: 241 IRAIRFLEKHFPLLCHDIRTGTLNSEITDQGIRESLRKILKPDPKLADFVEGECGKKCWG 300
           IRAIRFLEKH+ LLCHDIRTGTLNSEITDQGIRES+RKILK D KLADFVEGECGKKCWG
Sbjct: 241 IRAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKILKADGKLADFVEGECGKKCWG 300

Query: 301 GIISRIWPNTKYVDVIVTGTMSQYIKTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPE 360
           GIISRIWPNTKYVDVIVTGTMSQYI TLDYYSNGLPIVCTMYASSECYFGVNLKPMCKP 
Sbjct: 301 GIISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPG 360

Query: 361 EVAYTLIPTMAYFEFLPVNRSKGLSDKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRV 420
           EVAYTLIPTMAYFEFLPVNRSK   +KEL+KELVDLVDV+LG EYELVVTTY+GLYRYRV
Sbjct: 361 EVAYTLIPTMAYFEFLPVNRSK---EKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRV 420

Query: 421 GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYT 480
           GD+LRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL HLIPFDVTLSEYT
Sbjct: 421 GDILRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALTHLIPFDVTLSEYT 480

Query: 481 SCVDTSKIPGHYVLYWELTLNNNAIKTTPIPPSVFEDCCFTIEESLNAVYRQGRVSDKSI 540
           SCVDTS IPGHYVLYWELTLNN  IK+TPIPPSVFEDCCF IEESLNAVYRQGRVSDKSI
Sbjct: 481 SCVDTSNIPGHYVLYWELTLNNE-IKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSI 540

Query: 541 GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPK 600
           GPLEIKIVETGTFDKLMD+AISLGASINQYKTPRCVKYEPIIQLL+SRVVSNYFSPKCPK
Sbjct: 541 GPLEIKIVETGTFDKLMDFAISLGASINQYKTPRCVKYEPIIQLLSSRVVSNYFSPKCPK 600

Query: 601 WVPGHKQW 609
           WVP  KQW
Sbjct: 601 WVP--KQW 601

BLAST of Bhi09G001660 vs. ExPASy TrEMBL
Match: A0A6J1F6T2 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111442645 PE=3 SV=1)

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 552/609 (90.64%), Postives = 577/609 (94.75%), Query Frame = 0

Query: 1   MPEETPQKSSFLSSTTEYYKLLEKNKKTLQFIEDVTANADHVQRRVLTEILTRNADVEYL 60
           MPEE PQKSSFL STTEYYKLL++NKKTLQFIEDVTANA  VQRRVL EIL+RNA VEYL
Sbjct: 1   MPEEAPQKSSFL-STTEYYKLLQRNKKTLQFIEDVTANAAQVQRRVLAEILSRNAHVEYL 60

Query: 61  RRHGLGGATDRKTFKKTLPLISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGE 120
           RRHGL GATD KTFKKT+P+ISYEDIQ DINRIANGDSSPILCSSPISEFLTSSGTSGGE
Sbjct: 61  RRHGLAGATDPKTFKKTIPVISYEDIQHDINRIANGDSSPILCSSPISEFLTSSGTSGGE 120

Query: 121 RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT 180
           RKLMPTIEEELERRS LYSLLMPVMSQF+ GLEKGKGMYFLFIKSESKTPSGLLARPVLT
Sbjct: 121 RKLMPTIEEELERRSLLYSLLMPVMSQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLT 180

Query: 181 SYYKSSHFKHRPFDPYTNYTSPNQAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF 240
           SYYKS+HFKHRPFDPYTNYTSPN+AILCSD+YQSMYAQLLCGL HRTDVLRVGAVFASGF
Sbjct: 181 SYYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGF 240

Query: 241 IRAIRFLEKHFPLLCHDIRTGTLNSEITDQGIRESLRKILKPDPKLADFVEGECGKKCWG 300
           IRAIRFLEKHF LLCHDIRTGTLN +ITDQ IRESL+K LKPDPKLADF+EGEC KK W 
Sbjct: 241 IRAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTLKPDPKLADFIEGECSKKSWH 300

Query: 301 GIISRIWPNTKYVDVIVTGTMSQYIKTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPE 360
           GII+RIWPNTKYVDVIVTGTMSQYI TLDYYSNGLP+VCTMYASSECYFGVNL P+CKP 
Sbjct: 301 GIITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPS 360

Query: 361 EVAYTLIPTMAYFEFLPVNRSKGLSDKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRV 420
           EV+YTLIPTMAYFEFLPVNR+  L++KELQ ELVDLVDVQLGQEYELVVTTYAGLYRYRV
Sbjct: 361 EVSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRV 420

Query: 421 GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYT 480
           GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL+HL+PFD T+SEYT
Sbjct: 421 GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALSHLVPFDATVSEYT 480

Query: 481 SCVDTSKIPGHYVLYWELTLNNNAIKTTPIPPSVFEDCCFTIEESLNAVYRQGRVSDKSI 540
           SC DTSKIPGHYVLYWEL++  N  KTTPIPPSVFEDCC TIEESLNAVYRQGRVSDKSI
Sbjct: 481 SCADTSKIPGHYVLYWELSV--NGAKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSI 540

Query: 541 GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPK 600
           GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPK
Sbjct: 541 GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPK 600

Query: 601 WVPGHKQWN 610
           WVPGHKQW+
Sbjct: 601 WVPGHKQWS 606

BLAST of Bhi09G001660 vs. ExPASy TrEMBL
Match: A0A6J1IM75 (indole-3-acetic acid-amido synthetase GH3.6 OS=Cucurbita maxima OX=3661 GN=LOC111476851 PE=3 SV=1)

HSP 1 Score: 1117.8 bits (2890), Expect = 0.0e+00
Identity = 547/609 (89.82%), Postives = 573/609 (94.09%), Query Frame = 0

Query: 1   MPEETPQKSSFLSSTTEYYKLLEKNKKTLQFIEDVTANADHVQRRVLTEILTRNADVEYL 60
           MPEE PQKSSFL STTEYYKLL++NKKTLQFIEDVTANA  +QRRVL EIL+RNA VEYL
Sbjct: 1   MPEEAPQKSSFL-STTEYYKLLQRNKKTLQFIEDVTANAAQIQRRVLAEILSRNAHVEYL 60

Query: 61  RRHGLGGATDRKTFKKTLPLISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGE 120
           RRHGL GATD KTFK T+P+ISYEDIQ DINRIANGD SPILCSSPISEFLTSSGTSGGE
Sbjct: 61  RRHGLAGATDSKTFKNTIPVISYEDIQHDINRIANGDFSPILCSSPISEFLTSSGTSGGE 120

Query: 121 RKLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLT 180
           RKLMPTIEEELERRS LYSLLMPVMSQF+ GLEKGKGMYFLFIKSESKTPSGLLARPVLT
Sbjct: 121 RKLMPTIEEELERRSLLYSLLMPVMSQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLT 180

Query: 181 SYYKSSHFKHRPFDPYTNYTSPNQAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGF 240
           SYYKS+HFKHRPFDPYTNYTSPN+AILCSD+YQSMYAQLLCGL HRTDVLRVGAVFASGF
Sbjct: 181 SYYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGF 240

Query: 241 IRAIRFLEKHFPLLCHDIRTGTLNSEITDQGIRESLRKILKPDPKLADFVEGECGKKCWG 300
           IRAIRFLEKHF LLCHDIRTGTLN +ITDQ IRESL+K LKPDPKLADF+EGEC KK W 
Sbjct: 241 IRAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTLKPDPKLADFIEGECSKKSWH 300

Query: 301 GIISRIWPNTKYVDVIVTGTMSQYIKTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPE 360
           GII+RIWPNTKYVDVIVTGTMSQYI TLDYYSNGLP+VCTMYASSECYFGVNL P+CKP 
Sbjct: 301 GIITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPA 360

Query: 361 EVAYTLIPTMAYFEFLPVNRSKGLSDKELQKELVDLVDVQLGQEYELVVTTYAGLYRYRV 420
           EV+YTLIPTMAYFEFLPVNR+  L++KELQ ELVDLVDVQLGQEYELVVTTYAGLYRYRV
Sbjct: 361 EVSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRV 420

Query: 421 GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALNHLIPFDVTLSEYT 480
           GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNAL+HL+PFD T+SEYT
Sbjct: 421 GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALSHLVPFDATVSEYT 480

Query: 481 SCVDTSKIPGHYVLYWELTLNNNAIKTTPIPPSVFEDCCFTIEESLNAVYRQGRVSDKSI 540
           SC DTSKIPGHYVLYWEL++  N  KTT IPPSVFEDCC TIEESLNAVYRQGRVSDKSI
Sbjct: 481 SCADTSKIPGHYVLYWELSV--NGAKTTLIPPSVFEDCCLTIEESLNAVYRQGRVSDKSI 540

Query: 541 GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPK 600
           GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSR VSNYFSPKCPK
Sbjct: 541 GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRAVSNYFSPKCPK 600

Query: 601 WVPGHKQWN 610
           WVPGHKQW+
Sbjct: 601 WVPGHKQWS 606

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G54510.18.4e-29281.10Auxin-responsive GH3 family protein [more]
AT4G27260.11.4e-28377.67Auxin-responsive GH3 family protein [more]
AT2G14960.18.9e-22565.02Auxin-responsive GH3 family protein [more]
AT4G37390.11.1e-22262.30Auxin-responsive GH3 family protein [more]
AT2G23170.16.8e-21762.97Auxin-responsive GH3 family protein [more]
Match NameE-valueIdentityDescription
Q9LSQ41.2e-29081.10Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
O818292.0e-28277.67Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q8LQM51.5e-24067.81Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo... [more]
Q60EJ64.4e-22965.54Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo... [more]
O823331.3e-22365.02Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A5A7V8A50.0e+0094.08Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BYK90.0e+0094.08indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103494... [more]
A0A0A0LAR60.0e+0093.91Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G431430 PE=3 SV=1[more]
A0A6J1F6T20.0e+0090.64indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1IM750.0e+0089.82indole-3-acetic acid-amido synthetase GH3.6 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 32..578
e-value: 3.3E-202
score: 673.1
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 15..608
NoneNo IPR availablePANTHERPTHR31901:SF70BNAC03G14570D PROTEINcoord: 15..608

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi09M001660Bhi09M001660mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0010279 indole-3-acetic acid amido synthetase activity