Bhi09G001469 (gene) Wax gourd (B227) v1

Overview
NameBhi09G001469
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptiontwo-pore potassium channel 1-like
Locationchr9: 47758814 .. 47762328 (+)
RNA-Seq ExpressionBhi09G001469
SyntenyBhi09G001469
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCGATATTTGCACGGATCCGAACATTTCCATCCACAGGGTGGTTTTAAATAATTCCGTTGAATTCAACCAATATTTTATTATTGAACTCATCAAGCGGTGAGAATGAAAACGGAGATGAATGCATTCGCCGGCTACCACTGTTGCCTAATTTCTTCATTCGAATTCAACCAGAAAAAATACTCAGACCACCACAATTTTCAAACAAGGATAGCGAAATCCAAAGCGAAAGGTTTTCGATATCGAAATATTTACAAGCATATCGACCTCAAATCTTCTCCAAATCGGATGCTAATCCGATTCTAATTCAACGCCATTCACTGTAAGCTTCAGTTCTTCTCAGATCTTGCCGTAATCTGCTCATTAGCTTAATGAAATCTGTCCCGAGATCCACGTTTTCGTTTTTTACAAACTCAATTGAAGCTTCATTTGAATGTAATAAGATCTACTAAGGCGTAATTACTGAGTTTTTGGCTTCTTTTGTGGAGCTTTTTTCTATTAATTATTTATGTTCATTGGGAGTTGGTTTCCTTATAGATCGTGGAAATAGTGGATGATGGTCGAAATTAGTGCTTGACTTTTTGTTGAATTGTTGTTAAACTTAAACCTGTAGATGGGAGGATCTATATCTATTTCTTCAGTCTCTCGAATATATTGGACTCGGATGCTACATATAGTGTTTTCTTTTTTTGGAAGTTGTTACGCTTGTAAATTTGATTCATAAAATCACCAAGGTAGAAAAAGGCATATTGTACTTGAAACTTCAACTGAACTGAGCACTGGAATTAATTTTGAAATTGGAATTCACTGACTACCATTTTTCCTAGTTGCCCTTTATCTCAACCTTTTCTGAACTCGATGATAGACCTCCCAAAAGGGATTTTGTTTGATTATATTCTGAACTTAGATGTTGACGGGTATGAATGTTATGATAATGATAAGTTATGTTGATTGAGATAACATGTCAAATATAGTAGATTCTATTCCTTATTGAGAAAATTAGTTGAAAAGTTTCGGTATGGATAATACATCATATGTGAACGAAATGAAAGTGATGCTAGGACAAAACATAGAAAGTTATACATAACTCTGAGGCAGGGACACATATATGGTTTGTAATGTCACCCTTCTTGATTGAAAAATTATGTATCTCAATCTCAAAACAAAAGAATTTGATGTCTAATTGTTTTTCTCACATTTCAATATCTTTAGAAAGAAAATAAGAATTGATAAAGAACGATGGTTCTCTTCATGGGGGTCAATAAGACTAATCAAAGAATTTTAAGATTGGGGAATTCACAAGGGAAAAAACTTGAGAGAAAGAGAGAGCTTTTGCATTATGAAACGCCTATACATTGTGTAATCAAGTCTTCTTTGTTAGTATTTTAGGATAAACCTGGAATTGGATTTCATTTTGCTGATATTTTGAAGAGGTCTTATGCTTGACTTTGAGACGGATTATCAGAACAAAGGTTGAAGTCTTGTTGGTTTTGCAGACAGAGCTTGCATTTTAGGCGGAATTCCATAGTGAAAGTTTTTTTCAGAATTAAAATGGAAACTAACTGGAAATGAAAATCACTTGTTAAGTTGGTGTTTTGACTCTTAACTTAATGTAGAAGCTTCATACTGTTATGTTTTCGTTCATATCTTTCACTGTGATGATAATTCTAGTTTTTATTAAGGCAGAACTTTAATGCTGAGGAATAGGATTTTGTCACTGAATTTATGCATCGCTAAACTGAGTAACTTGGTTTTGAATATTATTGTGGCCATGCAGGTAACCGTTGGTTGTTTGCTGAATTATCTTAATGGGTAGCCAAGAAGCTAGACAGCCACTGTTACCAACATCATCTAATACTCCTGGAAAAAGGTTAATAGATATTCCTAGAAGCAGAAGACGATTGAGGCGCACTAAGAGTGCTCCTTATGCAGATTCTCCTCATACAGAGATCGCCTGCACTGGCAACGTGCCAGCCACTTGTCCAGTTCCACGTTCAGGATCAATTTTCAGAAATCTACACCCAAGTTTCAGAAGAGTAGCCTTAGTTTTAATCATATACCTGGGTATAGGAACTTTATGTTTTTACCTTGTCAGGTACCAAATCAAAGGGGAAAAAACAAACGGAATAGTTGATGCCATTTATTTTACTATTGTGACAATGACAACTGTAGGATATGGAGATCTTGTCCCAAACAGTCCTTCCACAAAACTACTAGCTTGTGCTTTCGTCTTCACAGGAATGGCCCTAATTGGCCTAATACTGAGCAATGCAGCCGATTATTTGGTAGAGAAGCAGGAAACATTGCTATTTAAAGCCTTCCATATGCACCAAAATAGCCATTGTGACATTTCAAGAGAAATTGATACTAATAAAGCTAGAAACAAGTGTATAGTGGTGTTTCTCATTCTTCTTTTGTTCATCATTTCTGGGACTGCCTTTCTAGTTTCGCTTGAGAAATTAGATTTTATTGATGCCTTTTATTGTGTATGTTCTACCATCACAACGCTAGGCTATGGAGATCAAAGCTTCTCGACCATGTGGGGACGTCTATTTGCCATTTTCTGGATCTTGATAAGTACCATCACTCTAGCTCAGTTTTTCCTTTACATTGCTGAGCTGAACACTGAAAGAAGGCAAAAGTCTCTGGTAAAGTGGATTCTAAGTAAAAAGGTGACAGACATAGACCTCGAGGTTGCAGATCTCGATGATGATGGGGTTGTTGGGTAAGTCATAGTCTGTTCTTTTCATCACACTCCCCTATCATGTGATCTCAAACTCACACGGTCAGGACACATCAATTCATTTCTGATGAGACCTCTTAACACGTTCCACCGTTTCTGAGTTGGAGTTTATTGAATTTCTTTAGTGGTTCACTTATCGTTTCCTTCTAAATCTTCCACAGGGCTGCAGAATTTGTCATATACAAGCTCAAGGAAATGGGGAAGATTACTGAAGATGACATTTCACCTGTGTTGGAGGAATTTGAGAATCTTGATGTTGATCAATCAGGAACTCTGTCTACATCTGATATAACACTTGCTCAATCATCTTAACCAGAGAGGGTGATGGTGGCCTTCCTTCTATCGCACCAGTTAAGTTGTTTTTGCAATGTTTTTATATTGCTTGTCTTGCTGCTTCTTATCTTGAGGAATGTGAATCATTCAATATTCTTTTGGAGATTGGTTATTGTCCATATGTTGTGGTAAAGAATATCCCTCCTGTTAGTGAAATGTTACAAAATTTCACTATAGTAAAGTTGAATATGAATTTTGTGACTTGATTGACGACATGAATGCATGGTAAGAGATTTAAGAAAATATTAAGGGAATATCATGAACTTGAATAGTATAGACCAAAATTTAATTGTATCCAACATTAGGGAAAATTGGCTACATTCCATGTTGAGGGGTTTTCCCCATTTATTTTCTTTTACCATTTCATAGCATTGTACAGATTTGGCATCTTTTGAGTTAAACCCTTGCCAGGTGGAGAAATGATAATTGGTGTCTTATCC

mRNA sequence

ATCGATATTTGCACGGATCCGAACATTTCCATCCACAGGGTGGTTTTAAATAATTCCGTTGAATTCAACCAATATTTTATTATTGAACTCATCAAGCGGTGAGAATGAAAACGGAGATGAATGCATTCGCCGGCTACCACTGTTGCCTAATTTCTTCATTCGAATTCAACCAGAAAAAATACTCAGACCACCACAATTTTCAAACAAGGATAGCGAAATCCAAAGCGAAAGGTTTTCGATATCGAAATATTTACAAGCATATCGACCTCAAATCTTCTCCAAATCGGATGCTAATCCGATTCTAATTCAACGCCATTCACTGTAACCGTTGGTTGTTTGCTGAATTATCTTAATGGGTAGCCAAGAAGCTAGACAGCCACTGTTACCAACATCATCTAATACTCCTGGAAAAAGGTTAATAGATATTCCTAGAAGCAGAAGACGATTGAGGCGCACTAAGAGTGCTCCTTATGCAGATTCTCCTCATACAGAGATCGCCTGCACTGGCAACGTGCCAGCCACTTGTCCAGTTCCACGTTCAGGATCAATTTTCAGAAATCTACACCCAAGTTTCAGAAGAGTAGCCTTAGTTTTAATCATATACCTGGGTATAGGAACTTTATGTTTTTACCTTGTCAGGTACCAAATCAAAGGGGAAAAAACAAACGGAATAGTTGATGCCATTTATTTTACTATTGTGACAATGACAACTGTAGGATATGGAGATCTTGTCCCAAACAGTCCTTCCACAAAACTACTAGCTTGTGCTTTCGTCTTCACAGGAATGGCCCTAATTGGCCTAATACTGAGCAATGCAGCCGATTATTTGGTAGAGAAGCAGGAAACATTGCTATTTAAAGCCTTCCATATGCACCAAAATAGCCATTGTGACATTTCAAGAGAAATTGATACTAATAAAGCTAGAAACAAGTGTATAGTGGTGTTTCTCATTCTTCTTTTGTTCATCATTTCTGGGACTGCCTTTCTAGTTTCGCTTGAGAAATTAGATTTTATTGATGCCTTTTATTGTGTATGTTCTACCATCACAACGCTAGGCTATGGAGATCAAAGCTTCTCGACCATGTGGGGACGTCTATTTGCCATTTTCTGGATCTTGATAAGTACCATCACTCTAGCTCAGTTTTTCCTTTACATTGCTGAGCTGAACACTGAAAGAAGGCAAAAGTCTCTGGTAAAGTGGATTCTAAGTAAAAAGGTGACAGACATAGACCTCGAGGTTGCAGATCTCGATGATGATGGGGTTGTTGGGGCTGCAGAATTTGTCATATACAAGCTCAAGGAAATGGGGAAGATTACTGAAGATGACATTTCACCTGTGTTGGAGGAATTTGAGAATCTTGATGTTGATCAATCAGGAACTCTGTCTACATCTGATATAACACTTGCTCAATCATCTTAACCAGAGAGGGTGATGGTGGCCTTCCTTCTATCGCACCAGTTAAGTTGTTTTTGCAATGTTTTTATATTGCTTGTCTTGCTGCTTCTTATCTTGAGGAATGTGAATCATTCAATATTCTTTTGGAGATTGGTTATTGTCCATATGTTGTGGTAAAGAATATCCCTCCTGTTAGTGAAATGTTACAAAATTTCACTATAGTAAAGTTGAATATGAATTTTGTGACTTGATTGACGACATGAATGCATGGTAAGAGATTTAAGAAAATATTAAGGGAATATCATGAACTTGAATAGTATAGACCAAAATTTAATTGTATCCAACATTAGGGAAAATTGGCTACATTCCATGTTGAGGGGTTTTCCCCATTTATTTTCTTTTACCATTTCATAGCATTGTACAGATTTGGCATCTTTTGAGTTAAACCCTTGCCAGGTGGAGAAATGATAATTGGTGTCTTATCC

Coding sequence (CDS)

ATGGGTAGCCAAGAAGCTAGACAGCCACTGTTACCAACATCATCTAATACTCCTGGAAAAAGGTTAATAGATATTCCTAGAAGCAGAAGACGATTGAGGCGCACTAAGAGTGCTCCTTATGCAGATTCTCCTCATACAGAGATCGCCTGCACTGGCAACGTGCCAGCCACTTGTCCAGTTCCACGTTCAGGATCAATTTTCAGAAATCTACACCCAAGTTTCAGAAGAGTAGCCTTAGTTTTAATCATATACCTGGGTATAGGAACTTTATGTTTTTACCTTGTCAGGTACCAAATCAAAGGGGAAAAAACAAACGGAATAGTTGATGCCATTTATTTTACTATTGTGACAATGACAACTGTAGGATATGGAGATCTTGTCCCAAACAGTCCTTCCACAAAACTACTAGCTTGTGCTTTCGTCTTCACAGGAATGGCCCTAATTGGCCTAATACTGAGCAATGCAGCCGATTATTTGGTAGAGAAGCAGGAAACATTGCTATTTAAAGCCTTCCATATGCACCAAAATAGCCATTGTGACATTTCAAGAGAAATTGATACTAATAAAGCTAGAAACAAGTGTATAGTGGTGTTTCTCATTCTTCTTTTGTTCATCATTTCTGGGACTGCCTTTCTAGTTTCGCTTGAGAAATTAGATTTTATTGATGCCTTTTATTGTGTATGTTCTACCATCACAACGCTAGGCTATGGAGATCAAAGCTTCTCGACCATGTGGGGACGTCTATTTGCCATTTTCTGGATCTTGATAAGTACCATCACTCTAGCTCAGTTTTTCCTTTACATTGCTGAGCTGAACACTGAAAGAAGGCAAAAGTCTCTGGTAAAGTGGATTCTAAGTAAAAAGGTGACAGACATAGACCTCGAGGTTGCAGATCTCGATGATGATGGGGTTGTTGGGGCTGCAGAATTTGTCATATACAAGCTCAAGGAAATGGGGAAGATTACTGAAGATGACATTTCACCTGTGTTGGAGGAATTTGAGAATCTTGATGTTGATCAATCAGGAACTCTGTCTACATCTGATATAACACTTGCTCAATCATCTTAA

Protein sequence

MGSQEARQPLLPTSSNTPGKRLIDIPRSRRRLRRTKSAPYADSPHTEIACTGNVPATCPVPRSGSIFRNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPSTKLLACAFVFTGMALIGLILSNAADYLVEKQETLLFKAFHMHQNSHCDISREIDTNKARNKCIVVFLILLLFIISGTAFLVSLEKLDFIDAFYCVCSTITTLGYGDQSFSTMWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWILSKKVTDIDLEVADLDDDGVVGAAEFVIYKLKEMGKITEDDISPVLEEFENLDVDQSGTLSTSDITLAQSS
Homology
BLAST of Bhi09G001469 vs. TAIR 10
Match: AT5G55630.1 (Outward rectifying potassium channel protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.3e-111
Identity = 214/368 (58.15%), Postives = 271/368 (73.64%), Query Frame = 0

Query: 1   MGSQEARQPLLPT------------SSNTPGKRLIDIPRSRRRLRRTKSAPYADSPHTEI 60
           M S  AR PLLPT            +S T   R       +RRLRR++SAP  D  + + 
Sbjct: 1   MSSDAARTPLLPTEKIDTMAQDFNLNSRTSSSR-------KRRLRRSRSAPRGDCMYNDD 60

Query: 61  ACTGNVPATCPVPRSGSIFRNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIV 120
                 P   P P    +F +L+P+ RRV + L +YL IGTLCFYLVR QI G KT+G+V
Sbjct: 61  VKIDEPP---PHPSKIPMFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVV 120

Query: 121 DAIYFTIVTMTTVGYGDLVPNSPSTKLLACAFVFTGMALIGLILSNAADYLVEKQETLLF 180
           DA+YF IVTMTTVGYGDLVPNS +++LLACAFVF+GM L+G +LS AADYLVEKQE LL 
Sbjct: 121 DALYFCIVTMTTVGYGDLVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLV 180

Query: 181 KAFHMHQN-SHCDISREIDTNKARNKCIVVFLILLLFIISGTAFLVSLEKLDFIDAFYCV 240
           +AFH+ Q+    DI +E+ TNK R KC    L+L++  I GT FLV +EK+  I AFYCV
Sbjct: 181 RAFHLRQSFGPTDILKELHTNKLRYKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCV 240

Query: 241 CSTITTLGYGDQSFSTMWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWILSK 300
           CST+TTLGYGD+SF++  GRLFA+FWIL S+I LAQFFLY+AELNTE +Q++LVKW+L++
Sbjct: 241 CSTVTTLGYGDKSFNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTR 300

Query: 301 KVTDIDLEVADLDDDGVVGAAEFVIYKLKEMGKITEDDISPVLEEFENLDVDQSGTLSTS 356
           ++T+ DLE ADLD+DGVVGAAEF++YKLKEMGKI E DIS +++EFE LD D+SGTL+TS
Sbjct: 301 RITNNDLEAADLDEDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTS 358

BLAST of Bhi09G001469 vs. TAIR 10
Match: AT5G55630.2 (Outward rectifying potassium channel protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.3e-111
Identity = 214/368 (58.15%), Postives = 271/368 (73.64%), Query Frame = 0

Query: 1   MGSQEARQPLLPT------------SSNTPGKRLIDIPRSRRRLRRTKSAPYADSPHTEI 60
           M S  AR PLLPT            +S T   R       +RRLRR++SAP  D  + + 
Sbjct: 1   MSSDAARTPLLPTEKIDTMAQDFNLNSRTSSSR-------KRRLRRSRSAPRGDCMYNDD 60

Query: 61  ACTGNVPATCPVPRSGSIFRNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIV 120
                 P   P P    +F +L+P+ RRV + L +YL IGTLCFYLVR QI G KT+G+V
Sbjct: 61  VKIDEPP---PHPSKIPMFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVV 120

Query: 121 DAIYFTIVTMTTVGYGDLVPNSPSTKLLACAFVFTGMALIGLILSNAADYLVEKQETLLF 180
           DA+YF IVTMTTVGYGDLVPNS +++LLACAFVF+GM L+G +LS AADYLVEKQE LL 
Sbjct: 121 DALYFCIVTMTTVGYGDLVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLV 180

Query: 181 KAFHMHQN-SHCDISREIDTNKARNKCIVVFLILLLFIISGTAFLVSLEKLDFIDAFYCV 240
           +AFH+ Q+    DI +E+ TNK R KC    L+L++  I GT FLV +EK+  I AFYCV
Sbjct: 181 RAFHLRQSFGPTDILKELHTNKLRYKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCV 240

Query: 241 CSTITTLGYGDQSFSTMWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWILSK 300
           CST+TTLGYGD+SF++  GRLFA+FWIL S+I LAQFFLY+AELNTE +Q++LVKW+L++
Sbjct: 241 CSTVTTLGYGDKSFNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTR 300

Query: 301 KVTDIDLEVADLDDDGVVGAAEFVIYKLKEMGKITEDDISPVLEEFENLDVDQSGTLSTS 356
           ++T+ DLE ADLD+DGVVGAAEF++YKLKEMGKI E DIS +++EFE LD D+SGTL+TS
Sbjct: 301 RITNNDLEAADLDEDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTS 358

BLAST of Bhi09G001469 vs. TAIR 10
Match: AT4G01840.1 (Ca2+ activated outward rectifying K+ channel 5 )

HSP 1 Score: 194.5 bits (493), Expect = 1.4e-49
Identity = 112/302 (37.09%), Postives = 178/302 (58.94%), Query Frame = 0

Query: 59  PVPRSGSIFRNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTM 118
           P P S SI        R+   +LI+YL +G   +   R    G +T+ +VDA+YF IVTM
Sbjct: 109 PSPVSKSI-------IRQAIFLLIVYLTLGVSIYSFNRDHYSGIETHPVVDALYFCIVTM 168

Query: 119 TTVGYGDLVPNSPSTKLLACAFVFTGMALIGLILSNAADYLVEKQETLLF-----KAFHM 178
            T+GYGD+ P +P TK+ A  FV  G   + ++LS   +Y+++ QE+++      +  H 
Sbjct: 169 CTIGYGDIAPLTPWTKIFAVVFVLFGFGFLDILLSGVVNYVLDLQESMILTGIQTRQHHQ 228

Query: 179 HQNSHCDISRE--IDTNKA----RNKCIVVFLILLLFIISGTAFLVSLEKLDFIDAFYCV 238
           H + H   +++  ID  K     R K  +   +++L I  G   L  +E+L F+D+ Y  
Sbjct: 229 HHHHHRFSAKDYIIDFEKGRMRIRMKVCLALCVVVLCIGVGALVLHFVEELGFVDSVYLS 288

Query: 239 CSTITTLGYGDQSFSTMWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWILSK 298
             ++TT+GYGD++F T+ GRLFA  W+L+ST+ +A+ FLY+AE   +RR +  VK  L++
Sbjct: 289 VMSVTTVGYGDRAFKTLQGRLFAAVWLLVSTLAVARAFLYLAEARIDRRHRKAVKLALNR 348

Query: 299 KVTDIDLEVADLDDDGVVGAAEFVIYKLKEMGKITEDDISPVLEEFENLDVDQSGTLSTS 350
           ++T  DL  AD    G +  +E+++ KLKEMGKIT+ DI  V+ +FE LD +Q G ++  
Sbjct: 349 EITVDDLLKADTYQHGFISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLP 403

BLAST of Bhi09G001469 vs. TAIR 10
Match: AT4G18160.1 (Ca2+ activated outward rectifying K+ channel 6 )

HSP 1 Score: 190.7 bits (483), Expect = 2.1e-48
Identity = 102/279 (36.56%), Postives = 167/279 (59.86%), Query Frame = 0

Query: 75  RRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPSTK 134
           R+   +L++YL +G L ++L R      +T+ +VD +YF IVTM T+GYGD+ PNS  TK
Sbjct: 151 RQAFALLVVYLSLGVLIYWLNRDHYVVNQTHPVVDGLYFCIVTMCTIGYGDITPNSVVTK 210

Query: 135 LLACAFVFTGMALIGLILSNAADYLVEKQETLLFKAFHMHQNSHCDISREIDTNKARN-- 194
           L +  FV  G   I ++LS    Y+++ QE+ +  +           S  ID  K R   
Sbjct: 211 LFSIMFVLVGFGFIDILLSGMVSYVLDLQESYMLDSAKRRDEPEKRRSYIIDVKKGRMRI 270

Query: 195 --KCIVVFLILLLFIISGTAFLVSLEKLDFIDAFYCVCSTITTLGYGDQSFSTMWGRLFA 254
             K  +   +++L I  G   +  +E++ ++D+FY    ++TT+GYGD++F T+ GRLFA
Sbjct: 271 RLKVALALGVVVLCIAVGVGIMHFIEEIGWLDSFYLSVMSVTTVGYGDRAFKTLPGRLFA 330

Query: 255 IFWILISTITLAQFFLYIAELNTERRQKSLVKWILSKKVTDIDLEVADLDDDGVVGAAEF 314
             W+L+ST+ +A+ FLY+AE   ++R +   K +L + ++      AD+D++G V  AE+
Sbjct: 331 AIWLLVSTLAVARAFLYLAEARVDKRNRERAKKVLCETMSVSQFFAADIDNNGCVSKAEY 390

Query: 315 VIYKLKEMGKITEDDISPVLEEFENLDVDQSGTLSTSDI 350
           VIYKLKEM KIT+ DI P+ ++F+ LD   +G ++  D+
Sbjct: 391 VIYKLKEMEKITDKDILPISKQFDKLDRCSNGKITLLDL 429

BLAST of Bhi09G001469 vs. TAIR 10
Match: AT5G46370.1 (Ca2+ activated outward rectifying K+ channel 2 )

HSP 1 Score: 176.4 bits (446), Expect = 4.0e-44
Identity = 100/278 (35.97%), Postives = 168/278 (60.43%), Query Frame = 0

Query: 80  VLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPSTKLLACA 139
           +L++YL +G L ++L R     ++T+ +VDA+YF IVTM T+GYGD+ P+S  TKL +  
Sbjct: 152 LLVVYLSLGVLIYWLNRDSYNVKQTHPVVDALYFCIVTMCTIGYGDITPDSVVTKLFSIF 211

Query: 140 FVFTGMALIGLILSNAADYLVEKQETLLFKAFH---MHQNSHCDI-SREIDTNKARN--- 199
           FV  G   + ++LS    Y+++ QE  + +      ++ N    + S  ID  K R    
Sbjct: 212 FVLVGFGFMDILLSGMVTYVLDLQENYMLETARNESLNLNDRDKVRSYIIDVKKGRMRIR 271

Query: 200 -KCIVVFLILLLFIISGTAFLVSLEKLDFIDAFYCVCSTITTLGYGDQSFSTMWGRLFAI 259
            K  +   +++L +  G   +  +EK+ ++D+FY    ++TT+GYGD++F+T+ GRL A 
Sbjct: 272 LKVGLALGVVVLCLGFGVLIMHFVEKIGWLDSFYFSVMSVTTVGYGDRAFNTLAGRLLAA 331

Query: 260 FWILISTITLAQFFLYIAELNTERRQKSLVKWILSKKVTDIDLEVADLDDDGVVGAAEFV 319
            W+L+ST+ +A+  L++AE   ++R +   K +L + ++      AD+D +G V  AEFV
Sbjct: 332 MWLLVSTLAVARAILFLAESRVDKRNRERAKKVLGESMSISQFLDADIDCNGCVSKAEFV 391

Query: 320 IYKLKEMGKITEDDISPVLEEFENLDVDQSGTLSTSDI 350
           IYKLK+M KITE DI+P+  +F+ LD   SG ++  D+
Sbjct: 392 IYKLKKMDKITEKDINPIGFQFDKLDRTNSGRITLLDL 429

BLAST of Bhi09G001469 vs. ExPASy Swiss-Prot
Match: Q8LBL1 (Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2)

HSP 1 Score: 400.6 bits (1028), Expect = 1.9e-110
Identity = 214/368 (58.15%), Postives = 271/368 (73.64%), Query Frame = 0

Query: 1   MGSQEARQPLLPT------------SSNTPGKRLIDIPRSRRRLRRTKSAPYADSPHTEI 60
           M S  AR PLLPT            +S T   R       +RRLRR++SAP  D  + + 
Sbjct: 1   MSSDAARTPLLPTEKIDTMAQDFNLNSRTSSSR-------KRRLRRSRSAPRGDCMYNDD 60

Query: 61  ACTGNVPATCPVPRSGSIFRNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIV 120
                 P   P P    +F +L+P+ RRV + L +YL IGTLCFYLVR QI G KT+G+V
Sbjct: 61  VKIDEPP---PHPSKIPMFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVV 120

Query: 121 DAIYFTIVTMTTVGYGDLVPNSPSTKLLACAFVFTGMALIGLILSNAADYLVEKQETLLF 180
           DA+YF IVTMTTVGYGDLVPNS +++LLACAFVF+GM L+G +LS AADYLVEKQE LL 
Sbjct: 121 DALYFCIVTMTTVGYGDLVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLV 180

Query: 181 KAFHMHQN-SHCDISREIDTNKARNKCIVVFLILLLFIISGTAFLVSLEKLDFIDAFYCV 240
           +AFH+ Q+    DI +E+ TNK R KC    L+L++  I GT FLV +EK+  I AFYCV
Sbjct: 181 RAFHLRQSFGPTDILKELHTNKLRYKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCV 240

Query: 241 CSTITTLGYGDQSFSTMWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWILSK 300
           CST+TTLGYGD+SF++  GRLFA+FWIL S+I LAQFFLY+AELNTE +Q++LVKW+L++
Sbjct: 241 CSTVTTLGYGDKSFNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTR 300

Query: 301 KVTDIDLEVADLDDDGVVGAAEFVIYKLKEMGKITEDDISPVLEEFENLDVDQSGTLSTS 356
           ++T+ DLE ADLD+DGVVGAAEF++YKLKEMGKI E DIS +++EFE LD D+SGTL+TS
Sbjct: 301 RITNNDLEAADLDEDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTS 358

BLAST of Bhi09G001469 vs. ExPASy Swiss-Prot
Match: Q850M0 (Two pore potassium channel a OS=Oryza sativa subsp. japonica OX=39947 GN=TPKA PE=1 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 1.3e-95
Identity = 189/356 (53.09%), Postives = 246/356 (69.10%), Query Frame = 0

Query: 1   MGSQEARQPLLPTSSNTPGKRLIDIPRSRRRLRRTKSAPYADSPHTEIACTGNVPATCPV 60
           M     +Q LL  + N   ++  +     RR R T S      P          P     
Sbjct: 1   MDDNSIQQSLLADNPNVLQRKPSEGVNRFRRCRSTPSTDPLQGP----------PEKGSS 60

Query: 61  PRSGSIFRNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120
            ++  +F+ + PSFR V L+L IYL +G L FY V  +I G++TN ++DA+YF +VTMTT
Sbjct: 61  VKAKELFKEMRPSFRLVGLLLFIYLLVGVLAFYAVMDEISGKRTNRVLDALYFCVVTMTT 120

Query: 121 VGYGDLVPNSPSTKLLACAFVFTGMALIGLILSNAADYLVEKQETLLFKAFHMH-QNSHC 180
           VGYGDLVPN+ +TKLLACAFVF GMA++ L +S  ADYLVEKQE L FKA H + +    
Sbjct: 121 VGYGDLVPNNDTTKLLACAFVFMGMAVVALFVSKVADYLVEKQEVLFFKALHTNLKGGET 180

Query: 181 DISREIDTNKARNKCIVVFLILLLFIISGTAFLVSLEKLDFIDAFYCVCSTITTLGYGDQ 240
            + R I+TN+ + K     L+L+L IISGT FL  +EKL  +D+FYCVC+TITTLGYGD+
Sbjct: 181 KMLRAIETNRIKYKFYTNALLLVLSIISGTVFLWKVEKLSLVDSFYCVCATITTLGYGDK 240

Query: 241 SFSTMWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWILSKKVTDIDLEVADL 300
           SFS+  GR+FA+FWI+ STI +AQFF+Y+AE+ TERRQK L  W+L++K+T +DLE ADL
Sbjct: 241 SFSSKLGRVFAVFWIITSTIIMAQFFMYLAEIYTERRQKMLANWVLTRKMTKMDLEAADL 300

Query: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLEEFENLDVDQSGTLSTSDITLAQSS 356
           DDD  VGAAEFV+YKLKE+GKI +++IS  LEEFE LDVD SGTLS  D+TLAQS+
Sbjct: 301 DDDRQVGAAEFVVYKLKELGKINQEEISSFLEEFEKLDVDHSGTLSPYDLTLAQSA 346

BLAST of Bhi09G001469 vs. ExPASy Swiss-Prot
Match: Q8LIN5 (Two pore potassium channel b OS=Oryza sativa subsp. japonica OX=39947 GN=TPKB PE=1 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 7.1e-86
Identity = 168/324 (51.85%), Postives = 223/324 (68.83%), Query Frame = 0

Query: 30  RRLRRTKSAPYADSPHTEIACTGNVPATCPVPRSGSIFRNLHPSFRRVALVLIIYLGIGT 89
           RR RR ++AP ++ P T+     +  +    P          PSFR V L+L+ YL +GT
Sbjct: 25  RRFRRCRTAPSSEPPPTD----KDNSSAADAPPKTLFTGGGRPSFRLVGLLLVAYLLLGT 84

Query: 90  LCFYLVRYQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPSTKLLACAFVFTGMALIG 149
           + FYL    + G +T   +DA+YF +VTMTTVGYGDLVP S + KLLACAFVF G+A++G
Sbjct: 85  IAFYLAMDHMSGTRTTRALDALYFCVVTMTTVGYGDLVPASDAAKLLACAFVFAGVAVVG 144

Query: 150 LILSNAADYLVEKQETLLFKAFHMHQNSHCDISREIDTNKARNKCIVVFLILLLFIISGT 209
             LS AADYLVEKQE LLF+A H H      + R ++ NK R K     L+L+  + SGT
Sbjct: 145 TFLSKAADYLVEKQEALLFRALHSH-----TMVRAMEMNKVRYKLYTAGLLLVAAVASGT 204

Query: 210 AFLVSLEKLDFIDAFYCVCSTITTLGYGDQSFSTMWGRLFAIFWILISTITLAQFFLYIA 269
             L  +E +  +DAFYCVC+T+TTLGYGD+SFS+  GR FA+ WI +ST+ +A FFLY A
Sbjct: 205 VVLWKVEGMRAVDAFYCVCATVTTLGYGDRSFSSEGGRAFAVAWITVSTVVVALFFLYAA 264

Query: 270 ELNTERRQKSLVKWILSKKVTDIDLEVADLDDDGVVGAAEFVIYKLKEMGKITEDDISPV 329
           EL TERRQ+ L +W+L ++ T++DLE ADLD D  VGAA+FV+YKLKE+GKI+++DIS  
Sbjct: 265 ELYTERRQRELARWVLRRRTTNMDLEAADLDGDHRVGAADFVLYKLKELGKISQEDISEF 324

Query: 330 LEEFENLDVDQSGTLSTSDITLAQ 354
           L+EF+NLD D SGTLS +D+  AQ
Sbjct: 325 LDEFDNLDADHSGTLSPADLAAAQ 339

BLAST of Bhi09G001469 vs. ExPASy Swiss-Prot
Match: Q9S6Z8 (Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.0e-48
Identity = 112/302 (37.09%), Postives = 178/302 (58.94%), Query Frame = 0

Query: 59  PVPRSGSIFRNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTM 118
           P P S SI        R+   +LI+YL +G   +   R    G +T+ +VDA+YF IVTM
Sbjct: 109 PSPVSKSI-------IRQAIFLLIVYLTLGVSIYSFNRDHYSGIETHPVVDALYFCIVTM 168

Query: 119 TTVGYGDLVPNSPSTKLLACAFVFTGMALIGLILSNAADYLVEKQETLLF-----KAFHM 178
            T+GYGD+ P +P TK+ A  FV  G   + ++LS   +Y+++ QE+++      +  H 
Sbjct: 169 CTIGYGDIAPLTPWTKIFAVVFVLFGFGFLDILLSGVVNYVLDLQESMILTGIQTRQHHQ 228

Query: 179 HQNSHCDISRE--IDTNKA----RNKCIVVFLILLLFIISGTAFLVSLEKLDFIDAFYCV 238
           H + H   +++  ID  K     R K  +   +++L I  G   L  +E+L F+D+ Y  
Sbjct: 229 HHHHHRFSAKDYIIDFEKGRMRIRMKVCLALCVVVLCIGVGALVLHFVEELGFVDSVYLS 288

Query: 239 CSTITTLGYGDQSFSTMWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWILSK 298
             ++TT+GYGD++F T+ GRLFA  W+L+ST+ +A+ FLY+AE   +RR +  VK  L++
Sbjct: 289 VMSVTTVGYGDRAFKTLQGRLFAAVWLLVSTLAVARAFLYLAEARIDRRHRKAVKLALNR 348

Query: 299 KVTDIDLEVADLDDDGVVGAAEFVIYKLKEMGKITEDDISPVLEEFENLDVDQSGTLSTS 350
           ++T  DL  AD    G +  +E+++ KLKEMGKIT+ DI  V+ +FE LD +Q G ++  
Sbjct: 349 EITVDDLLKADTYQHGFISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLP 403

BLAST of Bhi09G001469 vs. ExPASy Swiss-Prot
Match: Q69TN4 (Two pore potassium channel c OS=Oryza sativa subsp. japonica OX=39947 GN=TPKC PE=3 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.3e-47
Identity = 121/345 (35.07%), Postives = 192/345 (55.65%), Query Frame = 0

Query: 22  LIDIPRSRRRLRRTKSAPYADSPHTEIACTGNVPATCPVPRSGSIFRNLHPSFRRVALV- 81
           L D    R  L R+++AP A +P        N  A      SG   RN  P  RR A+V 
Sbjct: 106 LFDFIGGRTNLHRSRTAP-AMAPL-------NAAAIAAAAASGD-SRNPPPPPRRPAIVL 165

Query: 82  -----LIIYLGIGTLCFYLV---RYQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPS 141
                L+ YL +G + FY      +      T+ + DA+YF IVT+ T+GYGD+ P +P+
Sbjct: 166 HAFLFLLAYLAMG-VTFYAALPGNFTSSAGPTHPVADALYFCIVTLCTIGYGDITPATPA 225

Query: 142 TKLLACAFVFTGMALIGLILSNAADYLVEKQETLLFKAF--------HMHQNSHCDISRE 201
            KL + +FV  G   + ++LS    Y+++ QE LL  A         H H N   D+ + 
Sbjct: 226 AKLFSISFVLIGFGFVDILLSGMVSYVLDLQEHLLITALKNPRSVRKHRH-NYIFDLKK- 285

Query: 202 IDTNKARNKCIVVFLILLLFIISGTAFLVSLEKLDFIDAFYCVCSTITTLGYGDQSFSTM 261
               + R K  +   ++ + +  G A L  +E L ++DA Y    ++TT+GYGD +F T+
Sbjct: 286 -GRMRVRMKVALALTVVAICVGVGAAVLKRVENLGWLDAVYLAVMSVTTVGYGDHAFRTL 345

Query: 262 WGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWILSKKVTDIDLEVADLDDDGV 321
            GRLFA  W+L+ST+ +A+ FLY+AE+  ++R +++  W+LS+ +T  +   AD+D++G 
Sbjct: 346 AGRLFASAWLLVSTLAVARAFLYLAEMRIDKRHRAMANWVLSRDMTVSEFLAADIDNNGY 405

Query: 322 VGAAEFVIYKLKEMGKITEDDISPVLEEFENLDVDQSGTLSTSDI 350
           V  +EFV+YKLKEMGKI+E DI  + ++F+ +D    G ++ SD+
Sbjct: 406 VTKSEFVVYKLKEMGKISEKDIMMICDQFQRMDSGNCGKITLSDL 437

BLAST of Bhi09G001469 vs. ExPASy TrEMBL
Match: A0A5D3C7V3 (Two-pore potassium channel 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G00580 PE=3 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 3.8e-175
Identity = 322/355 (90.70%), Postives = 339/355 (95.49%), Query Frame = 0

Query: 1   MGSQEARQPLLPTSSNTPGKRLIDIPRSRRRLRRTKSAPYADSPHTEIACTGNVPATCPV 60
           MGS++ARQPLLPTSSNT   R+IDIPRS+RRLRRTKSAP+A+SP TEI  T NVPAT PV
Sbjct: 1   MGSEDARQPLLPTSSNTLETRVIDIPRSKRRLRRTKSAPHANSP-TEITRTSNVPATGPV 60

Query: 61  PRSGSIFRNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSG IF NLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT
Sbjct: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPSTKLLACAFVFTGMALIGLILSNAADYLVEKQETLLFKAFHMHQNSHCD 180
           VGYGDLVPNSPSTKLLACAFVF+GMAL+GLILSNAADYLVEKQE LLFKAFH+HQN HCD
Sbjct: 121 VGYGDLVPNSPSTKLLACAFVFSGMALVGLILSNAADYLVEKQEILLFKAFHIHQNGHCD 180

Query: 181 ISREIDTNKARNKCIVVFLILLLFIISGTAFLVSLEKLDFIDAFYCVCSTITTLGYGDQS 240
           IS+EIDTNKARNKC+VVFL+LLLFIISGTAFLV +EKLDFIDAFYCVCSTITTLGYGDQS
Sbjct: 181 ISKEIDTNKARNKCVVVFLLLLLFIISGTAFLVIIEKLDFIDAFYCVCSTITTLGYGDQS 240

Query: 241 FSTMWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWILSKKVTDIDLEVADLD 300
           FST WGR+FAIFWILISTITLAQFFLYIAELNTERRQKSLVKW+LSKKVTDIDLEVAD+D
Sbjct: 241 FSTKWGRIFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADID 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLEEFENLDVDQSGTLSTSDITLAQSS 356
           DDGVVGAAEFVIYKLKEMGKITEDDISPVL+EFENLDVDQSGTLS SDITLAQ S
Sbjct: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQLS 354

BLAST of Bhi09G001469 vs. ExPASy TrEMBL
Match: A0A1S3CI53 (two-pore potassium channel 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501240 PE=3 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 4.2e-174
Identity = 320/355 (90.14%), Postives = 339/355 (95.49%), Query Frame = 0

Query: 1   MGSQEARQPLLPTSSNTPGKRLIDIPRSRRRLRRTKSAPYADSPHTEIACTGNVPATCPV 60
           MGS++ARQPLLPTSSNT   R+IDIPRS+RRLRRTKSAP+A+SP TEI  T NVPAT PV
Sbjct: 1   MGSKDARQPLLPTSSNTLETRVIDIPRSKRRLRRTKSAPHANSP-TEITRTSNVPATGPV 60

Query: 61  PRSGSIFRNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSG IF NLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEK+NGIVDAIYFTIVTMTT
Sbjct: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKSNGIVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPSTKLLACAFVFTGMALIGLILSNAADYLVEKQETLLFKAFHMHQNSHCD 180
           VGYGDLVPNSPSTKLLACAFVF+GMAL+GLILSNAADYLVEKQE LLFKAFH+HQN HCD
Sbjct: 121 VGYGDLVPNSPSTKLLACAFVFSGMALVGLILSNAADYLVEKQEILLFKAFHIHQNGHCD 180

Query: 181 ISREIDTNKARNKCIVVFLILLLFIISGTAFLVSLEKLDFIDAFYCVCSTITTLGYGDQS 240
           IS+EIDTNKARNKC+VVFL+LLLFIISGTAFLV +EKLDFIDAFYCVCSTITTLGYGDQS
Sbjct: 181 ISKEIDTNKARNKCVVVFLLLLLFIISGTAFLVIIEKLDFIDAFYCVCSTITTLGYGDQS 240

Query: 241 FSTMWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWILSKKVTDIDLEVADLD 300
           FST WGR+FAIFWILISTITLAQFFLYIAELNTERRQKSLVKW+LS+KVTDIDLEVAD+D
Sbjct: 241 FSTKWGRIFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSEKVTDIDLEVADID 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLEEFENLDVDQSGTLSTSDITLAQSS 356
           DDGVVGAAEFVIYKLKEMGKITEDDISPVL+EFENLDVDQSGTLS SDITLAQ S
Sbjct: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQLS 354

BLAST of Bhi09G001469 vs. ExPASy TrEMBL
Match: A0A0A0K3G0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G098760 PE=3 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.7e-170
Identity = 317/355 (89.30%), Postives = 335/355 (94.37%), Query Frame = 0

Query: 1   MGSQEARQPLLPTSSNTPGKRLIDIPRSRRRLRRTKSAPYADSPHTEIACTGNVPATCPV 60
           MGSQ+ARQPLLPTSSNT   R+I+IPRS+RRLRRTKSAP+A+SP TEI  T NVPAT PV
Sbjct: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSP-TEITHTSNVPATGPV 60

Query: 61  PRSGSIFRNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSG IF NLHPSFRRVALVLI YLGIGTLCFYLVR+QI+GEKTN +VDAIYFTIVTMTT
Sbjct: 61  PRSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPSTKLLACAFVFTGMALIGLILSNAADYLVEKQETLLFKAFHMHQNSHCD 180
           VGYGDLVPNSPSTKLLACAFVFTGMAL+GLILSNAADYLVEKQE LLFKAFH+ QN HCD
Sbjct: 121 VGYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHIDQNGHCD 180

Query: 181 ISREIDTNKARNKCIVVFLILLLFIISGTAFLVSLEKLDFIDAFYCVCSTITTLGYGDQS 240
           IS+EIDTNKARNKCIVVFL+LLLFIISGTAFLV++EKLDFIDAFYCVCSTITTLGYGDQS
Sbjct: 181 ISKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQS 240

Query: 241 FSTMWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWILSKKVTDIDLEVADLD 300
           FST WGR+FAIFWILISTITLAQFFLYIAELNTERRQKSLVKW+LSKKVTDIDLEVAD+D
Sbjct: 241 FSTKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADID 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLEEFENLDVDQSGTLSTSDITLAQSS 356
           DDGVVGAAEFVIYKLKEMGKITEDDIS VL EFENLDVDQSGTLS SDITLAQ S
Sbjct: 301 DDGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS 354

BLAST of Bhi09G001469 vs. ExPASy TrEMBL
Match: A0A6J1IWT2 (two-pore potassium channel 1-like OS=Cucurbita maxima OX=3661 GN=LOC111480615 PE=3 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 9.1e-161
Identity = 294/355 (82.82%), Postives = 326/355 (91.83%), Query Frame = 0

Query: 1   MGSQEARQPLLPTSSNTPGKRLIDIPRSRRRLRRTKSAPYADSPHTEIACTGNVPATCPV 60
           M S+E  +PLLPTSSNT G R++DIPRS+ RLRRTKSAP+ADSPH+E   TG    TCPV
Sbjct: 1   MASKEVIEPLLPTSSNTIGTRILDIPRSKGRLRRTKSAPHADSPHSETTGTGTGADTCPV 60

Query: 61  PRSGSIFRNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSG IF NL PS RRVALVLI+Y+GIGTLCFYLVRYQI+GEKTNGIVDAIYFTIVTMTT
Sbjct: 61  PRSGFIFGNLGPSLRRVALVLILYMGIGTLCFYLVRYQIRGEKTNGIVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPSTKLLACAFVFTGMALIGLILSNAADYLVEKQETLLFKAFHMHQNSHCD 180
           VGYGDLVPNSPSTKLLACAFVF+GMAL+GLILS+AADYLVEKQE LLFK  + HQN HCD
Sbjct: 121 VGYGDLVPNSPSTKLLACAFVFSGMALVGLILSSAADYLVEKQEILLFKTLNAHQNGHCD 180

Query: 181 ISREIDTNKARNKCIVVFLILLLFIISGTAFLVSLEKLDFIDAFYCVCSTITTLGYGDQS 240
           +++EIDT+KARNKCIVVFL+L+LFIISGT FLV++EKL FIDAFYCVCSTITTLGYGD+S
Sbjct: 181 MTKEIDTDKARNKCIVVFLLLILFIISGTVFLVTIEKLGFIDAFYCVCSTITTLGYGDKS 240

Query: 241 FSTMWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWILSKKVTDIDLEVADLD 300
           FST  GR+FAIFWILISTITLAQFFLYIAELNTERRQKSLVKW+LSKKVT++DLE ADLD
Sbjct: 241 FSTKSGRIFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTNLDLEAADLD 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLEEFENLDVDQSGTLSTSDITLAQSS 356
           +D VVGAAEFVI+KLKEMGKITEDDI+PVL+EFE LDVDQSGTLSTSD+TLAQSS
Sbjct: 301 NDRVVGAAEFVIHKLKEMGKITEDDIAPVLKEFEKLDVDQSGTLSTSDLTLAQSS 355

BLAST of Bhi09G001469 vs. ExPASy TrEMBL
Match: A0A6J1FCF3 (two-pore potassium channel 1 OS=Cucurbita moschata OX=3662 GN=LOC111442740 PE=3 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 4.5e-160
Identity = 300/355 (84.51%), Postives = 322/355 (90.70%), Query Frame = 0

Query: 1   MGSQEARQPLLPTSSNTPGKRLIDIPRSRRRLRRTKSAPYADSPHTEIACTGNVPATCPV 60
           MGSQE RQP+LPTSSNT   R IDIPRS+RRLRRTKSAP++ SPHTE   T    AT  V
Sbjct: 1   MGSQEVRQPMLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSGSPHTETTST----ATGLV 60

Query: 61  PRSGSIFRNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSG +F NLHPSF RVALVLI+YLGIGTLCFYLV +QIKG KTNG+VDAIYFTIVTMTT
Sbjct: 61  PRSGLVFGNLHPSFIRVALVLIMYLGIGTLCFYLVGHQIKGGKTNGVVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPSTKLLACAFVFTGMALIGLILSNAADYLVEKQETLLFKAFHMHQNSHCD 180
           VGYGDLVPN+PSTKLLACAFVFTGMA++GLIL+NAADYLVEKQE  LFKAFHMHQN   D
Sbjct: 121 VGYGDLVPNTPSTKLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKAFHMHQNGPFD 180

Query: 181 ISREIDTNKARNKCIVVFLILLLFIISGTAFLVSLEKLDFIDAFYCVCSTITTLGYGDQS 240
           I++EIDTNKARNKCIVVFL LLLFII GT FLV+LE+LDFIDAFYCVCSTITTLGYGD+S
Sbjct: 181 ITKEIDTNKARNKCIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKS 240

Query: 241 FSTMWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWILSKKVTDIDLEVADLD 300
           FST WGRLFAIFWILIS+ITLAQFFLYIA LNTERR+KSLVKW+LSKKVT +DLE ADLD
Sbjct: 241 FSTRWGRLFAIFWILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLD 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLEEFENLDVDQSGTLSTSDITLAQSS 356
           DDGVVGAAEFVIYKLKEMGKITEDDISPVL+EFENLDVDQSGTLS SDITLAQSS
Sbjct: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQSS 351

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G55630.11.3e-11158.15Outward rectifying potassium channel protein [more]
AT5G55630.21.3e-11158.15Outward rectifying potassium channel protein [more]
AT4G01840.11.4e-4937.09Ca2+ activated outward rectifying K+ channel 5 [more]
AT4G18160.12.1e-4836.56Ca2+ activated outward rectifying K+ channel 6 [more]
AT5G46370.14.0e-4435.97Ca2+ activated outward rectifying K+ channel 2 [more]
Match NameE-valueIdentityDescription
Q8LBL11.9e-11058.15Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2[more]
Q850M01.3e-9553.09Two pore potassium channel a OS=Oryza sativa subsp. japonica OX=39947 GN=TPKA PE... [more]
Q8LIN57.1e-8651.85Two pore potassium channel b OS=Oryza sativa subsp. japonica OX=39947 GN=TPKB PE... [more]
Q9S6Z82.0e-4837.09Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1[more]
Q69TN41.3e-4735.07Two pore potassium channel c OS=Oryza sativa subsp. japonica OX=39947 GN=TPKC PE... [more]
Match NameE-valueIdentityDescription
A0A5D3C7V33.8e-17590.70Two-pore potassium channel 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3CI534.2e-17490.14two-pore potassium channel 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501240 ... [more]
A0A0A0K3G01.7e-17089.30Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G098760 PE=3 SV=1[more]
A0A6J1IWT29.1e-16182.82two-pore potassium channel 1-like OS=Cucurbita maxima OX=3661 GN=LOC111480615 PE... [more]
A0A6J1FCF34.5e-16084.51two-pore potassium channel 1 OS=Cucurbita moschata OX=3662 GN=LOC111442740 PE=3 ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003280Two pore domain potassium channelPRINTSPR013332POREKCHANELcoord: 232..241
score: 45.77
coord: 116..144
score: 29.17
IPR003280Two pore domain potassium channelPANTHERPTHR11003POTASSIUM CHANNEL, SUBFAMILY Kcoord: 21..350
IPR013099Potassium channel domainPFAMPF07885Ion_trans_2coord: 200..271
e-value: 5.3E-15
score: 55.1
coord: 80..160
e-value: 1.6E-16
score: 60.0
NoneNo IPR availableGENE3D1.10.287.70coord: 71..166
e-value: 1.8E-22
score: 81.0
NoneNo IPR availableGENE3D1.10.287.70coord: 176..284
e-value: 7.0E-20
score: 72.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR11003:SF270TWO PORE POTASSIUM CHANNEL Acoord: 21..350
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 187..292
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 70..175
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 298..310
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 283..350

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi09M001469Bhi09M001469mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030007 cellular potassium ion homeostasis
biological_process GO:0071257 cellular response to electrical stimulus
biological_process GO:0097623 potassium ion export across plasma membrane
biological_process GO:1990573 potassium ion import across plasma membrane
biological_process GO:0010029 regulation of seed germination
biological_process GO:0010119 regulation of stomatal movement
biological_process GO:0030322 stabilization of membrane potential
biological_process GO:0071805 potassium ion transmembrane transport
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0009705 plant-type vacuole membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022841 potassium ion leak channel activity
molecular_function GO:0005267 potassium channel activity