Bhi09G001399 (gene) Wax gourd (B227) v1

Overview


Name	Bhi09G001399
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	1,4-alpha-glucan branching enzyme
Location	chr9: 45024761 .. 45026202 (+)
RNA-Seq Expression	Bhi09G001399
Synteny	Bhi09G001399

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTCTGTATGCTGAAAATCACAGCCAAGTAATTTTCTTCCTAACATCTTTAATTATTAATCATCCATGGTTTAATGAATTATGAGCATGGATTTTACATGCTTGGTTGTGTGTTTCTTCCTATCTCTTCTTTACTTCTTTATCCTTTTAGTCCATCTCTAGTGGCGTTCAGTATGCCGAGATTCTGTTTGGTGATATCAAGGAGCATGCTGCTGGATCAAAGGAAACACTGCTCAGGGGGTTCCTTACACAAAGTACGATTCGTTTCTGAATTAAATTATTCCTTGATATTTTAGAAAATAAATGTTCTAGTTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAGCAGAAAACCTTCTAAGCCTCAAAACAGTTGCAAATACCAATGACAACGAGTGTACTGGTTCAAATGTTAGGTTAGCCATAGTCAGGGTTAGGAATTAGAACGTAGACCAAATGGTATGCTGATCCCACAACATTTTAACTAATCATCTTCAGTTTTCTTTTTGACATTAACTTTATCATAAATGATAATATTATATAACAGAACCTCTCGAAACCTGTTAAAAAAGATGGTTCCATCCAAGGTTCTTATCGTGTGTCTATGCCCATTTTCAGTGTTTATTAGCTTGCATTCAAATGCATAAGGTCAACTAGATACTGCAGAAATAGTTCTGCCTTCTATTCTTTTCTGTCCTTACCTACTGTTATTTAGTTTATCCATTTATGTAATTTTCTTTTGATGGCCTTCAATCAGATGATTAGATTGATCACATTTACTATTGGTGGACGAGCATACCTTAACTTCATGGGAAATGAATTTGGGCACCCAAAGGTCAGCTTCTATCTCTTCAATATGCATGAAGATGTATAATATTTTCTCCTGTCAAAATATATGTGTAATATTTGTCTATGGATCAGAGAGTTGAGTTCCCGATGCCGAGCAACAACTTCTCGTTTACGCTTGCTAACCGACAGTGGGATCTTTTAGAAAAGGAAATGCATCATGATTTGTTCCTTTTTGATAAGGTGTGTAGTTATTCTTCGACATCGTTAATATTGAAACTTTGTCATGCAGAGTGAAGTAAATGTTCGACCTTAAAGTCCCACAAATTTTTTATTATCTCATCTTAATTTAATAAAAAAAACTTACCTCAAAAAATAAATTGCCGTTTGTATAATAATTAAGTTGTGTTAATTTACTTTCTTCTCTCTAGGAGCTGATGAGATTGGATGAGAATGAAAAACTGCTGACAAGAAGGTTACCAAACGTTCACCATGTCAATGAATCTACCAAGGTAAGCTCTCTCCTTTTCCTTTCATTTCGAGTTCATTTTGTCTACTTGGAAATTTACACGTTTAGTACTTGTTTGTTATTGCTTTTGATTCTTTCATGCATCAGGTTTCAATTAATGTTTGTAAAGTAAGATACCTGACCTAA

mRNA sequence

ATGCTTCTGTATGCTGAAAATCACAGCCAATCCATCTCTAGTGGCGTTCAGTATGCCGAGATTCTGTTTGGTGATATCAAGGAGCATGCTGCTGGATCAAAGGAAACACTGCTCAGGGGGTTCCTTACACAAACTTGCATTCAAATGCATAAGATGATTAGATTGATCACATTTACTATTGGTGGACGAGCATACCTTAACTTCATGGGAAATGAATTTGGGCACCCAAAGAGAGTTGAGTTCCCGATGCCGAGCAACAACTTCTCGTTTACGCTTGCTAACCGACAGTGGGATCTTTTAGAAAAGGAAATGCATCATGATTTGTTCCTTTTTGATAAGGAGCTGATGAGATTGGATGAGAATGAAAAACTGCTGACAAGAAGGTTACCAAACGTTCACCATGTCAATGAATCTACCAAGGTTTCAATTAATGTTTGTAAAGTAAGATACCTGACCTAA

Coding sequence (CDS)

ATGCTTCTGTATGCTGAAAATCACAGCCAATCCATCTCTAGTGGCGTTCAGTATGCCGAGATTCTGTTTGGTGATATCAAGGAGCATGCTGCTGGATCAAAGGAAACACTGCTCAGGGGGTTCCTTACACAAACTTGCATTCAAATGCATAAGATGATTAGATTGATCACATTTACTATTGGTGGACGAGCATACCTTAACTTCATGGGAAATGAATTTGGGCACCCAAAGAGAGTTGAGTTCCCGATGCCGAGCAACAACTTCTCGTTTACGCTTGCTAACCGACAGTGGGATCTTTTAGAAAAGGAAATGCATCATGATTTGTTCCTTTTTGATAAGGAGCTGATGAGATTGGATGAGAATGAAAAACTGCTGACAAGAAGGTTACCAAACGTTCACCATGTCAATGAATCTACCAAGGTTTCAATTAATGTTTGTAAAGTAAGATACCTGACCTAA

Protein sequence

MLLYAENHSQSISSGVQYAEILFGDIKEHAAGSKETLLRGFLTQTCIQMHKMIRLITFTIGGRAYLNFMGNEFGHPKRVEFPMPSNNFSFTLANRQWDLLEKEMHHDLFLFDKELMRLDENEKLLTRRLPNVHHVNESTKVSINVCKVRYLT

Homology

[Close]

BLAST of Bhi09G001399 vs. TAIR 10
Match: AT3G20440.1 (Alpha amylase family protein )

HSP 1 Score: 186.4 bits (472), Expect = 1.7e-47
Identity = 92/141 (65.25%), Postives = 110/141 (78.01%), Query Frame = 0


Query: 1   MLLYAENHSQSISSGVQYAEILFGDIKEHAAGSKETLLRGFLTQTCIQMHKMIRLITFTI 60
           ML YAENH+QSIS G  +AEILFG +   + G KE L RG      I +HKMIRLITFT 
Sbjct: 644 MLSYAENHNQSISGGRSFAEILFGGVDNGSPGGKELLDRG------ISLHKMIRLITFTS 703

Query: 61  GGRAYLNFMGNEFGHPKRVEFPMPSNNFSFTLANRQWDLLEKEMHHDLFLFDKELMRLDE 120
           GGRAYLNFMGNEFGHP+RVEFP  SNNFSF+LANR+WDLLE  +HH LF FDKELM LD+
Sbjct: 704 GGRAYLNFMGNEFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLFSFDKELMDLDK 763

Query: 121 NEKLLTRRLPNVHHVNESTKV 142
           ++ +L+R LP++HHVN++  V
Sbjct: 764 SKGILSRGLPSIHHVNDANMV 778

[Close]

BLAST of Bhi09G001399 vs. TAIR 10
Match: AT3G20440.2 (Alpha amylase family protein )

HSP 1 Score: 186.4 bits (472), Expect = 1.7e-47
Identity = 92/141 (65.25%), Postives = 110/141 (78.01%), Query Frame = 0


Query: 1   MLLYAENHSQSISSGVQYAEILFGDIKEHAAGSKETLLRGFLTQTCIQMHKMIRLITFTI 60
           ML YAENH+QSIS G  +AEILFG +   + G KE L RG      I +HKMIRLITFT 
Sbjct: 674 MLSYAENHNQSISGGRSFAEILFGGVDNGSPGGKELLDRG------ISLHKMIRLITFTS 733

Query: 61  GGRAYLNFMGNEFGHPKRVEFPMPSNNFSFTLANRQWDLLEKEMHHDLFLFDKELMRLDE 120
           GGRAYLNFMGNEFGHP+RVEFP  SNNFSF+LANR+WDLLE  +HH LF FDKELM LD+
Sbjct: 734 GGRAYLNFMGNEFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLFSFDKELMDLDK 793

Query: 121 NEKLLTRRLPNVHHVNESTKV 142
           ++ +L+R LP++HHVN++  V
Sbjct: 794 SKGILSRGLPSIHHVNDANMV 808

[Close]

BLAST of Bhi09G001399 vs. TAIR 10
Match: AT3G20440.3 (Alpha amylase family protein )

HSP 1 Score: 181.4 bits (459), Expect = 5.4e-46
Identity = 92/143 (64.34%), Postives = 110/143 (76.92%), Query Frame = 0


Query: 1   MLLYAENHSQSISSGVQYAEILFGDIKEHAAGSKETLLRGFLTQTCIQMHKMIRLITFTI 60
           ML YAENH+QSIS G  +AEILFG +   + G KE L RG      I +HKMIRLITFT 
Sbjct: 679 MLSYAENHNQSISGGRSFAEILFGGVDNGSPGGKELLDRG------ISLHKMIRLITFTS 738

Query: 61  GGRAYLNFMGNEFGHPKRVEFPMPSNNFSFTLANRQWDLLEKEMHHDLFLFDK--ELMRL 120
           GGRAYLNFMGNEFGHP+RVEFP  SNNFSF+LANR+WDLLE  +HH LF FDK  ELM L
Sbjct: 739 GGRAYLNFMGNEFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLFSFDKVSELMDL 798

Query: 121 DENEKLLTRRLPNVHHVNESTKV 142
           D+++ +L+R LP++HHVN++  V
Sbjct: 799 DKSKGILSRGLPSIHHVNDANMV 815

[Close]

BLAST of Bhi09G001399 vs. TAIR 10
Match: AT5G03650.1 (starch branching enzyme 2.2 )

HSP 1 Score: 79.7 bits (195), Expect = 2.2e-15
Identity = 52/154 (33.77%), Postives = 80/154 (51.95%), Query Frame = 0


Query: 4   YAENHSQSISSGVQYAEILFG-DIKEHAAGSKETLLRGFLTQTCIQMHKMIRLITFTIGG 63
           YAE+H Q++      A  L   D+ +  A  + +     L    I +HKMIRLIT  +GG
Sbjct: 569 YAESHDQALVGDKTIAFWLMDKDMYDFMAVDRPSTP---LIDRGIALHKMIRLITMGLGG 628

Query: 64  RAYLNFMGNEFGHPKRVEFP-----------MPSNNFSFTLANRQWDLLEKE--MHHDLF 123
             YLNFMGNEFGHP+ ++FP           +P NNFS+    R++DL + +   +  L 
Sbjct: 629 EGYLNFMGNEFGHPEWIDFPRGEQRLSDGSVIPGNNFSYDKCRRRFDLGDADYLRYRGLQ 688

Query: 124 LFDKELMRLDENEKLLTRRLPNVHHVNESTKVSI 144
            FD+ +  L+EN   +T     +   +E+ +V +
Sbjct: 689 EFDQAMQHLEENYGFMTSEHQFISRKDEADRVIV 719

[Close]

BLAST of Bhi09G001399 vs. TAIR 10
Match: AT2G36390.1 (starch branching enzyme 2.1 )

HSP 1 Score: 77.8 bits (190), Expect = 8.4e-15
Identity = 51/156 (32.69%), Postives = 79/156 (50.64%), Query Frame = 0


Query: 2   LLYAENHSQSISSGVQYAEILFG-DIKEHAAGSKETLLRGFLTQTCIQMHKMIRLITFTI 61
           ++YAE+H Q++      A  L   D+ +  A  ++   R       I +HKMIRLIT  +
Sbjct: 602 VVYAESHDQALVGDKTIAFWLMDKDMYDFMAVDRQATPR---VDRGIALHKMIRLITMGL 661

Query: 62  GGRAYLNFMGNEFGHPKRVEFP-----------MPSNNFSFTLANRQWDLLEKE--MHHD 121
           GG  YLNFMGNEFGHP+ ++FP           +  NN S+    R++DL + E   +H 
Sbjct: 662 GGEGYLNFMGNEFGHPEWIDFPRTDQHLPDGRVIAGNNGSYDKCRRRFDLGDAEYLRYHG 721

Query: 122 LFLFDKELMRLDENEKLLTRRLPNVHHVNESTKVSI 144
           L  FD+ +  L+E    +T     +   +E  +V +
Sbjct: 722 LQEFDRAMQNLEETYGFMTSEHQYISRKDEGDRVIV 754

[Close]

BLAST of Bhi09G001399 vs. ExPASy Swiss-Prot
Match: D2WL32 (1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SBE3 PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 2.4e-46
Identity = 92/141 (65.25%), Postives = 110/141 (78.01%), Query Frame = 0


Query: 1   MLLYAENHSQSISSGVQYAEILFGDIKEHAAGSKETLLRGFLTQTCIQMHKMIRLITFTI 60
           ML YAENH+QSIS G  +AEILFG +   + G KE L RG      I +HKMIRLITFT 
Sbjct: 674 MLSYAENHNQSISGGRSFAEILFGGVDNGSPGGKELLDRG------ISLHKMIRLITFTS 733

Query: 61  GGRAYLNFMGNEFGHPKRVEFPMPSNNFSFTLANRQWDLLEKEMHHDLFLFDKELMRLDE 120
           GGRAYLNFMGNEFGHP+RVEFP  SNNFSF+LANR+WDLLE  +HH LF FDKELM LD+
Sbjct: 734 GGRAYLNFMGNEFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLFSFDKELMDLDK 793

Query: 121 NEKLLTRRLPNVHHVNESTKV 142
           ++ +L+R LP++HHVN++  V
Sbjct: 794 SKGILSRGLPSIHHVNDANMV 808

[Close]

BLAST of Bhi09G001399 vs. ExPASy Swiss-Prot
Match: P30924 (1,4-alpha-glucan-branching enzyme OS=Solanum tuberosum OX=4113 GN=SBE1 PE=2 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 6.7e-17
Identity = 52/144 (36.11%), Postives = 73/144 (50.69%), Query Frame = 0


Query: 2   LLYAENHSQSISSGVQYAEILFGDIKEHAAGSKETLLRGFLTQTCIQMHKMIRLITFTIG 61
           + YAE+H QSI      A +L    KE  +G         +    I +HKMI   T  +G
Sbjct: 546 IAYAESHDQSIVGDKTIAFLLMD--KEMYSGMSCLTDASPVVDRGIALHKMIHFFTMALG 605

Query: 62  GRAYLNFMGNEFGHPKRVEFPMPSNNFSFTLANRQWDLLEKEMHHDLFL--FDKELMRLD 121
           G  YLNFMGNEFGHP+ ++FP   NN+S+    RQW+L + E     F+  FD+ +  LD
Sbjct: 606 GEGYLNFMGNEFGHPEWIDFPREGNNWSYDKCRRQWNLADSEHLRYKFMNAFDRAMNSLD 665

Query: 122 ENEKLLTRRLPNVHHVNESTKVSI 144
           E    L      V  +++  KV +
Sbjct: 666 EKFSFLASGKQIVSSMDDDNKVVV 687

[Close]

BLAST of Bhi09G001399 vs. ExPASy Swiss-Prot
Match: Q01401 (1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SBE1 PE=1 SV=2)

HSP 1 Score: 86.3 bits (212), Expect = 3.3e-16
Identity = 51/144 (35.42%), Postives = 73/144 (50.69%), Query Frame = 0


Query: 2   LLYAENHSQSISSGVQYAEILFGDIKEHAAGSKETLLRGFLTQTCIQMHKMIRLITFTIG 61
           + YAE+H QSI      A +L    KE   G  +           I + KMI  IT  +G
Sbjct: 526 IAYAESHDQSIVGDKTIAFLLMD--KEMYTGMSDLQPASPTINRGIALQKMIHFITMALG 585

Query: 62  GRAYLNFMGNEFGHPKRVEFPMPSNNFSFTLANRQWDLLEKE--MHHDLFLFDKELMRLD 121
           G  YLNFMGNEFGHP+ ++FP   NN+S+    RQW L++ +   +  +  FD+ +  L+
Sbjct: 586 GDGYLNFMGNEFGHPEWIDFPREGNNWSYDKCRRQWSLVDTDHLRYKYMNAFDQAMNALE 645

Query: 122 ENEKLLTRRLPNVHHVNESTKVSI 144
           E    L+     V  +NE  KV +
Sbjct: 646 EEFSFLSSSKQIVSDMNEKDKVIV 667

[Close]

BLAST of Bhi09G001399 vs. ExPASy Swiss-Prot
Match: Q04446 (1,4-alpha-glucan-branching enzyme OS=Homo sapiens OX=9606 GN=GBE1 PE=1 SV=3)

HSP 1 Score: 85.1 bits (209), Expect = 7.4e-16
Identity = 54/142 (38.03%), Postives = 78/142 (54.93%), Query Frame = 0


Query: 2   LLYAENHSQSISSGVQYAEILFGDIKEHAAGSKETLLRGFLTQTCIQMHKMIRLITFTIG 61
           + YAE+H Q++      A  L  D + +   S  T     + +  IQ+HKMIRLIT  +G
Sbjct: 474 IAYAESHDQALVGDKSLAFWLM-DAEMYTNMSVLTPFTPVIDRG-IQLHKMIRLITHGLG 533

Query: 62  GRAYLNFMGNEFGHPKRVEFPMPSNNFSFTLANRQWDLLEKEMHHDLFL--FDKELMRLD 121
           G  YLNFMGNEFGHP+ ++FP   NN S+  A RQ+ L + ++    FL  FD+++ RL+
Sbjct: 534 GEGYLNFMGNEFGHPEWLDFPRKGNNESYHYARRQFHLTDDDLLRYKFLNNFDRDMNRLE 593

Query: 122 ENEKLLTRRLPNVHHVNESTKV 142
           E    L      V   +E  K+
Sbjct: 594 ERYGWLAAPQAYVSEKHEGNKI 613

[Close]

BLAST of Bhi09G001399 vs. ExPASy Swiss-Prot
Match: Q6EAS5 (1,4-alpha-glucan-branching enzyme OS=Equus caballus OX=9796 GN=GBE1 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.3e-15
Identity = 54/142 (38.03%), Postives = 79/142 (55.63%), Query Frame = 0


Query: 2   LLYAENHSQSISSGVQYAEILFGDIKEHAAGSKETLLRGFLTQTCIQMHKMIRLITFTIG 61
           + YAE+H Q++      A  L  D + +   S  T     + +  IQ+HKMIRLIT  +G
Sbjct: 471 IAYAESHDQALVGDKSLAFWLM-DAEMYTNMSVLTPFTPVIDRG-IQLHKMIRLITHALG 530

Query: 62  GRAYLNFMGNEFGHPKRVEFPMPSNNFSFTLANRQWDLLEKEMHHDLFL--FDKELMRLD 121
           G  YLNFMGNEFGHP+ ++FP   NN S+  A RQ+ L + ++    FL  FD+++ +L+
Sbjct: 531 GEGYLNFMGNEFGHPEWLDFPRKGNNESYHYARRQFHLTDDDLLRYKFLNNFDRDMNKLE 590

Query: 122 ENEKLLTRRLPNVHHVNESTKV 142
           E    L+     V   +E  KV
Sbjct: 591 ERCGWLSAPQAFVSEKHEGNKV 610

[Close]

BLAST of Bhi09G001399 vs. ExPASy TrEMBL
Match: A0A0A0K8R4 (Alpha-amylase_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G213190 PE=4 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 1.6e-61
Identity = 121/141 (85.82%), Postives = 126/141 (89.36%), Query Frame = 0


Query: 1   MLLYAENHSQSISSGVQYAEILFGDIKEHAAGSKETLLRGFLTQTCIQMHKMIRLITFTI 60
           MLL+AENH QSIS G  YAEILFGDIKEH AGSKETLLRG        +HKMIRLITFTI
Sbjct: 88  MLLFAENHGQSISGGRSYAEILFGDIKEHGAGSKETLLRG------CSLHKMIRLITFTI 147

Query: 61  GGRAYLNFMGNEFGHPKRVEFPMPSNNFSFTLANRQWDLLEKEMHHDLFLFDKELMRLDE 120
           GGRAYLNFMGNEFGHPKRVEFPMPSNNFSF+LANRQWDLLEKEMHHDLFLFDKELM LDE
Sbjct: 148 GGRAYLNFMGNEFGHPKRVEFPMPSNNFSFSLANRQWDLLEKEMHHDLFLFDKELMGLDE 207

Query: 121 NEKLLTRRLPNVHHVNESTKV 142
           NEK+LTR LPNVHHVNE+TKV
Sbjct: 208 NEKILTRSLPNVHHVNETTKV 222

[Close]

BLAST of Bhi09G001399 vs. ExPASy TrEMBL
Match: A0A1S4DX00 (1,4-alpha-glucan branching enzyme OS=Cucumis melo OX=3656 GN=LOC103490131 PE=3 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 5.1e-60
Identity = 120/141 (85.11%), Postives = 125/141 (88.65%), Query Frame = 0


Query: 1   MLLYAENHSQSISSGVQYAEILFGDIKEHAAGSKETLLRGFLTQTCIQMHKMIRLITFTI 60
           MLL+AENHSQSIS G  YAEILFGDIKEH AGSKETLLRG        +HKMIRLITFTI
Sbjct: 687 MLLFAENHSQSISGGRSYAEILFGDIKEHGAGSKETLLRG------CSLHKMIRLITFTI 746

Query: 61  GGRAYLNFMGNEFGHPKRVEFPMPSNNFSFTLANRQWDLLEKEMHHDLFLFDKELMRLDE 120
           GGRAYLNFMGNEFGHPKRVEFPM SNNFSF+LANRQWDLLEKEMHH LFLFDKELM LDE
Sbjct: 747 GGRAYLNFMGNEFGHPKRVEFPMLSNNFSFSLANRQWDLLEKEMHHGLFLFDKELMGLDE 806

Query: 121 NEKLLTRRLPNVHHVNESTKV 142
           NEK+LTR LPNVHHVNE+TKV
Sbjct: 807 NEKILTRSLPNVHHVNETTKV 821

[Close]

BLAST of Bhi09G001399 vs. ExPASy TrEMBL
Match: A0A1S3BIZ8 (1,4-alpha-glucan branching enzyme OS=Cucumis melo OX=3656 GN=LOC103490131 PE=3 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 5.1e-60
Identity = 120/141 (85.11%), Postives = 125/141 (88.65%), Query Frame = 0


Query: 1   MLLYAENHSQSISSGVQYAEILFGDIKEHAAGSKETLLRGFLTQTCIQMHKMIRLITFTI 60
           MLL+AENHSQSIS G  YAEILFGDIKEH AGSKETLLRG        +HKMIRLITFTI
Sbjct: 653 MLLFAENHSQSISGGRSYAEILFGDIKEHGAGSKETLLRG------CSLHKMIRLITFTI 712

Query: 61  GGRAYLNFMGNEFGHPKRVEFPMPSNNFSFTLANRQWDLLEKEMHHDLFLFDKELMRLDE 120
           GGRAYLNFMGNEFGHPKRVEFPM SNNFSF+LANRQWDLLEKEMHH LFLFDKELM LDE
Sbjct: 713 GGRAYLNFMGNEFGHPKRVEFPMLSNNFSFSLANRQWDLLEKEMHHGLFLFDKELMGLDE 772

Query: 121 NEKLLTRRLPNVHHVNESTKV 142
           NEK+LTR LPNVHHVNE+TKV
Sbjct: 773 NEKILTRSLPNVHHVNETTKV 787

[Close]

BLAST of Bhi09G001399 vs. ExPASy TrEMBL
Match: A0A1S3BIP3 (1,4-alpha-glucan branching enzyme OS=Cucumis melo OX=3656 GN=LOC103490131 PE=3 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 5.1e-60
Identity = 120/141 (85.11%), Postives = 125/141 (88.65%), Query Frame = 0


Query: 1   MLLYAENHSQSISSGVQYAEILFGDIKEHAAGSKETLLRGFLTQTCIQMHKMIRLITFTI 60
           MLL+AENHSQSIS G  YAEILFGDIKEH AGSKETLLRG        +HKMIRLITFTI
Sbjct: 687 MLLFAENHSQSISGGRSYAEILFGDIKEHGAGSKETLLRG------CSLHKMIRLITFTI 746

Query: 61  GGRAYLNFMGNEFGHPKRVEFPMPSNNFSFTLANRQWDLLEKEMHHDLFLFDKELMRLDE 120
           GGRAYLNFMGNEFGHPKRVEFPM SNNFSF+LANRQWDLLEKEMHH LFLFDKELM LDE
Sbjct: 747 GGRAYLNFMGNEFGHPKRVEFPMLSNNFSFSLANRQWDLLEKEMHHGLFLFDKELMGLDE 806

Query: 121 NEKLLTRRLPNVHHVNESTKV 142
           NEK+LTR LPNVHHVNE+TKV
Sbjct: 807 NEKILTRSLPNVHHVNETTKV 821

[Close]

BLAST of Bhi09G001399 vs. ExPASy TrEMBL
Match: A0A5D3DIL6 (1,4-alpha-glucan-branching enzyme 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G00690 PE=4 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 3.3e-59
Identity = 119/141 (84.40%), Postives = 124/141 (87.94%), Query Frame = 0


Query: 1   MLLYAENHSQSISSGVQYAEILFGDIKEHAAGSKETLLRGFLTQTCIQMHKMIRLITFTI 60
           MLL+AENHSQSIS G  YAEILFGDIKEH AGSKETLLRG        +HKMIRLITFTI
Sbjct: 343 MLLFAENHSQSISGGRSYAEILFGDIKEHGAGSKETLLRG------CSLHKMIRLITFTI 402

Query: 61  GGRAYLNFMGNEFGHPKRVEFPMPSNNFSFTLANRQWDLLEKEMHHDLFLFDKELMRLDE 120
           GGRAYLNFMGNEFGHPKRVEFPM SNNFSF+LANRQWDLL KEMHH LFLFDKELM LDE
Sbjct: 403 GGRAYLNFMGNEFGHPKRVEFPMLSNNFSFSLANRQWDLLGKEMHHGLFLFDKELMGLDE 462

Query: 121 NEKLLTRRLPNVHHVNESTKV 142
           NEK+LTR LPNVHHVNE+TKV
Sbjct: 463 NEKILTRSLPNVHHVNETTKV 477

The following BLAST results are available for this feature:

BLAST of Bhi09G001399 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G20440.1	1.7e-47	65.25	Alpha amylase family protein	[more]
AT3G20440.2	1.7e-47	65.25	Alpha amylase family protein	[more]
AT3G20440.3	5.4e-46	64.34	Alpha amylase family protein	[more]
AT5G03650.1	2.2e-15	33.77	starch branching enzyme 2.2	[more]
AT2G36390.1	8.4e-15	32.69	starch branching enzyme 2.1	[more]

BLAST of Bhi09G001399 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
D2WL32	2.4e-46	65.25	1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic OS=Arabidopsis t...	[more]
P30924	6.7e-17	36.11	1,4-alpha-glucan-branching enzyme OS=Solanum tuberosum OX=4113 GN=SBE1 PE=2 SV=2	[more]
Q01401	3.3e-16	35.42	1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic OS=Oryza sativa su...	[more]
Q04446	7.4e-16	38.03	1,4-alpha-glucan-branching enzyme OS=Homo sapiens OX=9606 GN=GBE1 PE=1 SV=3	[more]
Q6EAS5	1.3e-15	38.03	1,4-alpha-glucan-branching enzyme OS=Equus caballus OX=9796 GN=GBE1 PE=1 SV=1	[more]

BLAST of Bhi09G001399 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K8R4	1.6e-61	85.82	Alpha-amylase_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G21...	[more]
A0A1S4DX00	5.1e-60	85.11	1,4-alpha-glucan branching enzyme OS=Cucumis melo OX=3656 GN=LOC103490131 PE=3 S...	[more]
A0A1S3BIZ8	5.1e-60	85.11	1,4-alpha-glucan branching enzyme OS=Cucumis melo OX=3656 GN=LOC103490131 PE=3 S...	[more]
A0A1S3BIP3	5.1e-60	85.11	1,4-alpha-glucan branching enzyme OS=Cucumis melo OX=3656 GN=LOC103490131 PE=3 S...	[more]
A0A5D3DIL6	3.3e-59	84.40	1,4-alpha-glucan-branching enzyme 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 1..126 e-value: 5.0E-27 score: 96.7
None	No IPR available	PANTHER	PTHR43651:SF4	1,4-ALPHA-GLUCAN-BRANCHING ENZYME 3, CHLOROPLASTIC/AMYLOPLASTIC	coord: 1..141
IPR037439	1,4-alpha-glucan-branching enzyme	PANTHER	PTHR43651	1,4-ALPHA-GLUCAN-BRANCHING ENZYME	coord: 1..141
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 2..126

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M001399	Bhi09M001399	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005978	glycogen biosynthetic process
biological_process	GO:0009791	post-embryonic development
biological_process	GO:0019252	starch biosynthetic process
cellular_component	GO:0009501	amyloplast
molecular_function	GO:0003844	1,4-alpha-glucan branching enzyme activity
molecular_function	GO:0102752	1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis)
molecular_function	GO:0043169	cation binding
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds