Bhi09G001071 (gene) Wax gourd (B227) v1

BLAST of Bhi09G001071 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 210.3 bits (534), Expect = 3.4e-54
Identity = 139/427 (32.55%), Postives = 226/427 (52.93%), Query Frame = 0

Query: 45  EGDDVVISIEKMIMELPSVNPNSSLYKVPKGHIKTNETMYTPRFISIGPFHHGQPELMAA 104
           EGD +V SI+  +  L S++    +YKVP    + N   YTPR +S GP H G+ EL A 
Sbjct: 271 EGDALVDSIKAKLAFLSSLSTKCCIYKVPNKLRRLNPDAYTPRLVSFGPLHRGKEELQAM 330

Query: 105 EQYKLWALKTYLCRAKMGVKEAMSLACSWEEKARSCYGHPIDMNKQNFAKMMLLDGCFIL 164
           E  K   L +++ R    +++ + LA +WE+ ARSCY   + ++   F +M+++DG F++
Sbjct: 331 EDQKYRYLLSFIPRTNSSLEDLVRLARTWEQNARSCYAEDVKLHSDEFVEMLVVDGSFLV 390

Query: 165 EFLNITNDLYIEDDEKWIDDGSLIYRILYDVERDLTIYENQLPFFVLQQLYDLLPFPPEN 224
           E L  ++   +  +   I   S+   ++ DV RD+ + ENQLPFFV+++++ LL    + 
Sbjct: 391 ELLLRSHYPRLRGENDRIFGNSM---MITDVCRDMILIENQLPFFVVKEIFLLLLNYYQQ 450

Query: 225 DDPAV------HFMECMSKLFIATTTGYSYHYNATTEVRHLVDLLKFYFIPS-PDTDEYK 284
             P++      HF   +S++               TE  H VDLL+  ++P  P   EY 
Sbjct: 451 GTPSIIQLAQRHFSYFLSRI---------DDEKFITEPEHFVDLLRSCYLPQFPIKLEYT 510

Query: 285 KFKESEEESYLESPNLTKLCEAGVKVQKADEAKSMMDFSFKNGILKIPPTIIHNVFEIHI 344
             K         +P  T+L  AGV+ + A+ +  ++D SF +G+LKIP  ++ ++ E   
Sbjct: 511 TVKVD------NAPEATELHTAGVRFKPAETSSCLLDISFADGVLKIPTIVVDDLTESLY 570

Query: 345 RNLIVSEIVSEDETYLFHYVALLDDLIDTEKDVSILVKEKIISNTSGCSDQQVAELINNL 404
           +N+I  E           Y+ LL   I +  D  +L+   II N  G +   V+ L N+ 
Sbjct: 571 KNIIGFEQCRCSNKNFLDYIMLLGCFIKSPTDADLLIHSGIIVNYLG-NSVDVSNLFNS- 630

Query: 405 RLNAPTFPDKCYYYNHMSKELNDYCNKWWHRSMASLRRDYFNTPWASISFFAATFLLILT 464
            ++     D+ +Y++ +S+ L  YCN  W+R  A LRRDYF+ PWA  S FAA  LL+LT
Sbjct: 631 -ISKEVIYDRRFYFSMLSENLQAYCNTPWNRWKAILRRDYFHNPWAVASVFAALLLLLLT 676

BLAST of Bhi09G001071 vs. TAIR 10
Match: AT2G36430.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 175.6 bits (444), Expect = 9.2e-44
Identity = 132/445 (29.66%), Postives = 226/445 (50.79%), Query Frame = 0

Query: 45  EGDDVV-ISIEKM---------IMELPSVNPNSSLYKVPKGHIKTNETMYTPRFISIGPF 104
           EG+ ++ I IE+M         ++   +  P  S+++VP+  I  N   Y PR +SIGP+
Sbjct: 13  EGESLLKIHIERMHKKLKEPPRLLSSAAGKPTCSIFRVPQSMIDCNGRCYEPRVVSIGPY 72

Query: 105 HHGQPELMAAEQYKLWALKTYLCRAK-MGVKEAMSLACSWEEKARSCYGHPIDMNKQNFA 164
           H GQ +L   E++K   L   L R + + +++ M    + EE AR CY   I M+ + F 
Sbjct: 73  HRGQTQLKMIEEHKWRYLNVLLTRTQNLTLEDYMKSVKNVEEVARECYSETIHMDSEEFN 132

Query: 165 KMMLLDGCFILEFLNITNDLYIEDDEKWIDDGSLIYRILYDVERDLTIYENQLPFFVLQQ 224
           +MM+LDGCF+LE     N+L   +    +   + +    Y   RD    ENQ+PFFVL+ 
Sbjct: 133 EMMVLDGCFLLELFRKVNNLVPFEPNDPLVAMAWVLPFFY---RDFLCLENQIPFFVLET 192

Query: 225 LYDLLPFPPENDDPAVHFMECMSKLF---IATTTGYSYHYNATTEVRHLVDLLKFYFIPS 284
           L++L     EN+  A   ++ ++  F   +   T            +HL+DLL+  FIP 
Sbjct: 193 LFNLTRGDNENETNA--SLQSLAFAFFNNMMHRTEEDLARFKELRAKHLLDLLRSSFIPE 252

Query: 285 PDTDEYKKFKESEEE--SYLESPNLTKLCEAGVKVQKADEAKSMMDFSFKNGILKIPPTI 344
            +          +E+  S++   +++KL  AG+K+++  +A+S +   F++G +++P   
Sbjct: 253 SELHTPPATNPGKEKMPSHI-IHSISKLRRAGIKLRELKDAESFLVVRFRHGTIEMPAIT 312

Query: 345 IHNVFEIHIRNLIVSEIVS-EDETYLFHYVALLDDLIDTEKDVSILVKEKIISNTSGCSD 404
           + +     + N +  E        +   Y  LLD L +T KDV  L  + II N  G +D
Sbjct: 313 VDDFMSSFLENCVAYEQCHVACSMHFTTYATLLDCLTNTYKDVEYLCDQNIIENYFG-TD 372

Query: 405 QQVAELINNLRLNAPTFPDKCYYYNHMSKELNDYCNKWWHRSMASLRRDYFNTPWASISF 464
            ++A+ +N+L  +      +C Y   + +E+N+Y    WH   A+ +  YFN+PW+ +S 
Sbjct: 373 TELAKFVNSLGRDVAFDITQC-YLKDLFEEVNEYYKSSWHVEWATFKFTYFNSPWSFVSA 432

Query: 465 FAATFLLILTFLQTLFSTPFIFYNK 473
            AA  LL+L+ +QT++ T F  Y K
Sbjct: 433 LAALVLLVLSVIQTIY-TVFQAYQK 448

BLAST of Bhi09G001071 vs. TAIR 10
Match: AT3G50150.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 174.5 bits (441), Expect = 2.0e-43
Identity = 149/475 (31.37%), Postives = 232/475 (48.84%), Query Frame = 0

Query: 17  DLEMSKKGEVEGDEEMIMENNNNNKDVEEGDDVVISIEKMIMELPSVNPNSS-----LYK 76
           DL  +    VE   E         K  E  ++ VISI+  + +  S +  +S     +Y+
Sbjct: 35  DLGQNLHNHVETYVEPSKIEVKEEKPRETREEWVISIKDKMEKALSYDATNSWDKLCIYR 94

Query: 77  VPKGHIKTNETMYTPRFISIGPFHHGQPELMAAEQYKLWALKTYLCRAKMGVKEAMSLAC 136
           VP    + ++  Y P+ +SIGP+HHG+  L   E++K  A+   + R K  ++  +    
Sbjct: 95  VPFYLQENDKKSYLPQTVSIGPYHHGKVHLRPMERHKWRAVNMIMARTKHNIEMYIDAMK 154

Query: 137 SWEEKARSCYGHPIDM-NKQNFAKMMLLDGCFILEFLNIT-----NDLYIEDDEKWIDDG 196
             EE+AR+CY  PIDM N   F +M++LDGCF+LE    T        Y  +D  +   G
Sbjct: 155 ELEEEARACYQGPIDMKNSNEFTEMLVLDGCFVLELFKGTIQGFQKIGYARNDPVFAKRG 214

Query: 197 SLIYRILYDVERDLTIYENQLPFFVLQQLYDLLPFPPEND----DPAVHFME-CMSKLFI 256
                +++ ++RD+ + ENQLP FVL +L  L    P       + AV F +  M    +
Sbjct: 215 -----LMHSIQRDMIMLENQLPLFVLDRLLGLQTGTPNQTGIVAEVAVRFFKTLMPTSEV 274

Query: 257 ATTTGYSYHYNATTEV------RHLVDLLKFYFIPSPDTDE----YKKFKESEEESYLES 316
            T +  S      ++        H +D+     I S +T      Y+     E++  L  
Sbjct: 275 LTKSERSLDSQEKSDELGDNGGLHCLDVFHRSLIQSSETTNQGTPYEDMSMVEKQQQL-I 334

Query: 317 PNLTKLCEAGVKVQKADEAKSMMDFSFKNGILKIPPTIIHNVFEIHIRNLIVSE-IVSED 376
             +T+L  AGV   +  E   + D  FKNG LKIP  +IH+  +    NLI  E   ++ 
Sbjct: 335 HCVTELRGAGVNFMR-KETGQLWDIEFKNGYLKIPKLLIHDGTKSLFSNLIAFEQCHTQS 394

Query: 377 ETYLFHYVALLDDLIDTEKDVSILVKEKIISNTSGCSDQQVAELINNLRLNAPTFPDKCY 436
              +  Y+  +D+LI++ +DVS L  + II +  G SD +VA+L N L      F  K  
Sbjct: 395 SNNITSYIIFMDNLINSSQDVSYLHHDGIIEHWLG-SDSEVADLFNRL-CKEVIFDPKDG 454

Query: 437 YYNHMSKELNDYCNKWWHRSMASLRRDYFNTPWASISFFAATFLLILTFLQTLFS 465
           Y + +S+E+N Y ++ W+   A+LR+ YFN PWA  SF AA  LL LTF Q+ F+
Sbjct: 455 YLSQLSREVNRYYSRKWNSLKATLRQKYFNNPWAYFSFSAAVILLFLTFFQSFFA 500

BLAST of Bhi09G001071 vs. TAIR 10
Match: AT3G50160.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 168.7 bits (426), Expect = 1.1e-41
Identity = 144/455 (31.65%), Postives = 224/455 (49.23%), Query Frame = 0

Query: 15  ESDLEMSKKGEVEGDEEMIMENNNNNKDVEEGDDVVISIEKMIMELPSVNPNSSLYKVPK 74
           ES + +  K E +  E  ++  N+  K +  GD+   S +           N  +Y+VP 
Sbjct: 65  ESVVSIEDKNEQKLREIWVISLNDKMKTL--GDNATTSWD-----------NLCIYRVPP 124

Query: 75  GHIKTNET-MYTPRFISIGPFHHGQPELMAAEQYKLWALKTYLCRAKMGVKEAMSLACSW 134
            +++ N+T  Y P+ +SIGP+HHG   LM  E++K  A+   + RAK  ++  +      
Sbjct: 125 -YLQENDTKSYMPQIVSIGPYHHGHKHLMPMERHKWRAVNMVMARAKHDIEMYIDAMKEL 184

Query: 135 EEKARSCYGHPIDMNKQNFAKMMLLDGCFILEFLNITNDLYIEDDEKWIDDGSLIYRILY 194
           EEKAR+CY  PI+MN+  F +M++LDG FI+E    T++ + E      D    +  ++ 
Sbjct: 185 EEKARACYQGPINMNRNEFIEMLVLDGVFIIEIFKGTSEGFQEIGYAPNDPVFGMRGLMQ 244

Query: 195 DVERDLTIYENQLPFFVLQQLYDLLPFPPENDDPAVHFMECMSKLFIATTTGYSYHYNAT 254
            + RD+ + ENQLP+ VL+ L   L  P   D   V   +   +  + T           
Sbjct: 245 SIRRDMVMLENQLPWSVLKGLLQ-LQRPDVLDKVNVQLFQPFFQPLLPTR-------EVL 304

Query: 255 TEV--RHLVDLLKFYFIPSPDT-DEYKKFKESEEESYLESPNLTKLCEAGVKVQKADEAK 314
           TE    H +D+L+   + S  T DE       + +  +    +T+L  AGV+  +  E  
Sbjct: 305 TEEGGLHCLDVLRRGLLQSSGTSDEDMSMVNKQPQQLIHC--VTELRNAGVEFMR-KETG 364

Query: 315 SMMDFSFKNGILKIPPTIIHNVFEIHIRNLIVSEIVS-EDETYLFHYVALLDDLIDTEKD 374
              D  FKNG LKIP  +IH+  +    NLI  E    +    +  Y+  +D+LI++ +D
Sbjct: 365 HFWDIEFKNGYLKIPKLLIHDGTKSLFLNLIAFEQCHIKSSKKITSYIIFMDNLINSSED 424

Query: 375 VSILVKEKIISNTSGCSDQQVAELINNLRLNAPTFPDKCYYYNHMSKELNDYCNKWWHRS 434
           VS L    II N  G SD +V++L N L      F     Y + ++ E+N Y  + W+  
Sbjct: 425 VSYLHHYGIIENWLG-SDSEVSDLFNGLGKEV-IFDPNDGYLSALTGEVNIYYRRKWNYL 484

Query: 435 MASLRRDYFNTPWASISFFAATFLLILTFLQTLFS 465
            A+LR  YFN PWA  SF AA  LLI TF Q+ F+
Sbjct: 485 KATLRHKYFNNPWAYFSFIAAVTLLIFTFCQSFFA 492

BLAST of Bhi09G001071 vs. TAIR 10
Match: AT3G50170.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 166.4 bits (420), Expect = 5.6e-41
Identity = 139/481 (28.90%), Postives = 234/481 (48.65%), Query Frame = 0

Query: 15  ESDLEMSKKGEVEGDEEMIMENNNNNKDVEEGDDVVISIEKMIMELPSVNPNS-----SL 74
           + +++  K+   E  +E++ E      +   GD  VISI   + +    +  +      +
Sbjct: 60  DQNVQNHKQSHSESGKEVVEE----RPEETTGDSWVISIRDKLEQADRDDDTTIWGKLCI 119

Query: 75  YKVPKGHIKTNETMYTPRFISIGPFHHGQPELMAAEQYKLWALKTYLCRAKMGVKEAMSL 134
           Y+VP    + ++  Y P+ +S+GP+HHG+  L   E++K  AL   L R K  ++   + 
Sbjct: 120 YRVPHYLQENDKKSYFPQTVSLGPYHHGKKRLRPMERHKWRALNKVLKRLKQRIEMYTNA 179

Query: 135 ACSWEEKARSCYGHPIDMNKQNFAKMMLLDGCFILEFLNITNDLYIEDDEKWIDDGSLIY 194
               EEKAR+CY  PI +++  F +M++LDGCF+LE    T + + E      D    + 
Sbjct: 180 MRELEEKARACYEGPISLSRNEFTEMLVLDGCFVLELFRGTVEGFTEIGYARNDPVFAMR 239

Query: 195 RILYDVERDLTIYENQLPFFVLQQLYDLLPFPPEND------------DPAVHFMECMSK 254
            +++ ++RD+ + ENQLP FVL +L + L    +N             DP +   E ++K
Sbjct: 240 GLMHSIQRDMIMLENQLPLFVLDRLLE-LQLGTQNQTGIVAHVAVKFFDPLMPTGEALTK 299

Query: 255 LFIATTTGY---SYHYNATTEVRHLVDLLKFYFI---PSPDTDEYKK-------FKESEE 314
              +    +   S          H +D+ +   +   P+P+T    K         +  +
Sbjct: 300 PDQSKLMNWLEKSLDTLGDKGELHCLDVFRRSLLQSSPTPNTRSLLKRLTRNTRVVDKRQ 359

Query: 315 ESYLESPNLTKLCEAGVKVQKADEAKSMMDFSFKNGILKIPPTIIHNVFEIHIRNLIVSE 374
           +  +    +T+L EAGVK +K  +     D  FKNG L+IP  +IH+  +    NLI  E
Sbjct: 360 QQLVHC--VTELREAGVKFRKR-KTDRFWDIEFKNGYLEIPKLLIHDGTKSLFSNLIAFE 419

Query: 375 IVS-EDETYLFHYVALLDDLIDTEKDVSILVKEKIISNTSGCSDQQVAELINNLRLNAPT 434
               E   ++  Y+  +D+LI++ +DVS L    II +  G SD +VA+L N L      
Sbjct: 420 QCHIESSNHITSYIIFMDNLINSSEDVSYLHYCGIIEHWLG-SDSEVADLFNRL-CQEVV 479

Query: 435 FPDKCYYYNHMSKELNDYCNKWWHRSMASLRRDYFNTPWASISFFAATFLLILTFLQTLF 465
           F  K  + + +S ++N Y N+ W+   A+L   YFN PWA  SF AA  LL+LT  Q+ +
Sbjct: 480 FDPKDSHLSRLSGDVNRYYNRKWNVLKATLTHKYFNNPWAYFSFSAAVILLLLTLCQSFY 530

BLAST of Bhi09G001071 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 2.0e-27
Identity = 108/418 (25.84%), Postives = 195/418 (46.65%), Query Frame = 0

Query: 69  LYKVPKGHIKTNETMYTPRFISIGPFHHGQPELMAAEQYKLWALKTYLCRA-KMGVKE-- 128
           +++VP+  +  N   Y P+ +SIGP+H+G+  L   +Q+K   L+ +L  A K  V+E  
Sbjct: 48  IFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAKKKDVEENV 107

Query: 129 AMSLACSWEEKARSCYGHPIDMNKQNFAKMMLLDGCFIL-EFLNITNDLYIEDDEKWIDD 188
            +      E+K R  Y   +     +   MM+LDGCFIL  FL ++ ++ + +D  +   
Sbjct: 108 LVKAVVDLEDKIRKSYSEELKTG-HDLMFMMVLDGCFILMVFLIMSGNIELSEDPIF--- 167

Query: 189 GSLIYRILYDVERDLTIYENQLPFFVLQQLY--DLLPFPPENDDPAVHFMECMSKLFIAT 248
              I  +L  ++ DL + ENQ+PFFVLQ LY    +    + +  A HF +         
Sbjct: 168 --SIPWLLSSIQSDLLLLENQVPFFVLQTLYVGSKIGVSSDLNRIAFHFFKNP-----ID 227

Query: 249 TTGYSYHYNATTEVRHLVDLLKFYFIPSPDTDEYKKFKESEEESYL---ESPNL------ 308
             G  +  +   + +HL+DL++  F+  P+T E  K      +  L   +S N+      
Sbjct: 228 KEGSYWEKHRNYKAKHLLDLIRETFL--PNTSESDKASSPHVQVQLHEGKSGNVPSVDSK 287

Query: 309 --------TKLCEAGVKVQ-KADEAKSMMDFSFKNGILKIPPTIIHNVFEIHIRNLIVSE 368
                    +L   G+K + +  +  S+++   K   L+IP             N +  E
Sbjct: 288 AVPLILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNKLQIPQLRFDGFISSFFLNCVAFE 347

Query: 369 -IVSEDETYLFHYVALLDDLIDTEKDVSILVKEKIISNTSGCSDQQVAELINNLRLNAPT 428
              ++    +  Y+  +  L++ E+DV+ L  +K+I      S+ +V+E    +  +   
Sbjct: 348 QFYTDSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVSEFFKTISKDV-V 407

Query: 429 FPDKCYYYNHMSKELNDYCNKWWHRSMASLRRDYFNTPWASISFFAATFLLILTFLQT 462
           F     Y N++ K +N+Y  KW++   A  R  +F +PW  +S  A  F+++LT LQ+
Sbjct: 408 FEVDTSYLNNVFKGVNEYTKKWYNGLWAGFRHTHFESPWTFLSSCAVLFVILLTMLQS 451

BLAST of Bhi09G001071 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.2e-11
Identity = 65/235 (27.66%), Postives = 107/235 (45.53%), Query Frame = 0

Query: 68  SLYKVPKGHIKTNETMYTPRFISIGPFHHGQPELMAAEQYKLW-ALKTYLCRAKMGVKEA 127
           S++ VPK  + ++   YTP  +SIGP+H  +PEL   E+YKL  A K           + 
Sbjct: 44  SIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPELHEMERYKLMIARKIRNQYNSFRFHDL 103

Query: 128 MSLACSWEEKARSCYGHPIDMNKQNFAKMMLLDGCFILEFLNITNDLYIEDDEKWIDDGS 187
           +    S E K R+CY   I  N +    +M +D  F++EFL I +   +E         +
Sbjct: 104 VEKLQSMEIKIRACYHKYIGFNGETLLWIMAVDSSFLIEFLKIYSFRKVE---------T 163

Query: 188 LIYRILY-DVERDLTIYENQLPFFVLQQLYDLLPFPPENDDPAVHFMECMSKLFIATTTG 247
           LI R+ + ++ RD+ + ENQ+P FVL++    L F  E+ + A         L ++  TG
Sbjct: 164 LINRVGHNEILRDIMMIENQIPLFVLRK---TLEFQLESTESA-------DDLLLSVLTG 223

Query: 248 YSYHYNATT--------------EVRHLVDLLKFYFIPSPDTDEYKKFKESEEES 287
                +                 E  H++D L    +P  + +E    +E +EE+
Sbjct: 224 LCKDLSPLVIKFDDDQILKAQFQECNHILDFLYQMIVPRIEEEE---LEEDDEEN 256

BLAST of Bhi09G001071 vs. ExPASy TrEMBL
Match: A0A0A0K6U9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G363030 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.9e-134
Identity = 266/462 (57.58%), Postives = 352/462 (76.19%), Query Frame = 0

Query: 25  EVEG-DEEMIMENNNNN--KDVEEG--DDVVISIEKMIMELPSVNPNSSLYKVPKGHIKT 84
           E+EG D++ +MEN      +D EE   DDVV+SIEKM M+LP +NP   +Y+VP+ H K 
Sbjct: 6   EIEGHDDQEMMENYKKAGFEDQEENNDDDVVVSIEKMFMKLPPINPKCFIYQVPELHRKM 65

Query: 85  NETMYTPRFISIGPFHHGQPELMAAEQYKLWALKTYLCRAKMGVKEAMSLACSWEEKARS 144
           N+  YTP+ ISIGP HHGQ +  AAEQYK++ALK YL R  M V EAM +A  WEE AR+
Sbjct: 66  NDIAYTPQLISIGPLHHGQQQYKAAEQYKVYALKRYLSRINMTVGEAMDIAGRWEETARN 125

Query: 145 CYGHPIDMNKQNFAKMMLLDGCFILEFLNIT---NDLYIEDDEKWIDDGSLIYRILYDVE 204
           CY +PIDMN++ F KMMLLD CFILEF+N T      + ++++ W++D +L+  +L+ ++
Sbjct: 126 CYANPIDMNREEFVKMMLLDSCFILEFMNSTVCRLRYWGQNEDHWMND-TLLCGLLFGLK 185

Query: 205 RDLTIYENQLPFFVLQQLYDLLPFPPEN--DDPA-VHFMECMSKLFIATTTGYSYHYNA- 264
           RDL+++ENQLPFF LQ+L+  L F  +N  + P+ + F+  M+ LFIATT GY    N  
Sbjct: 186 RDLSMFENQLPFFPLQELF-YLSFNRQNVTNPPSPIIFLTSMAHLFIATTGGYQLRCNVL 245

Query: 265 --TTEVRHLVDLLKFYFIPSPDTDEYKKFKESEEESYLESPNLTKLCEAGVKVQKADEAK 324
             T +VRHLVDLL+FY+IPSPDT+EYK + E+E+ SY+ +P +++LCEAGV++Q+AD+AK
Sbjct: 246 DDTHKVRHLVDLLRFYYIPSPDTEEYKSY-EAEKSSYINTPTISELCEAGVQIQRADDAK 305

Query: 325 SMMDFSFKNGILKIPPTIIHNVFEIHIRNLIVSEIV--SEDETYLFHYVALLDDLIDTEK 384
           S++DF+FK+G+LKIPP  IH  FEI IRNLIVSEI   ++D  ++F Y+ALLDDLI+TEK
Sbjct: 306 SLLDFNFKDGVLKIPPFNIHIEFEIQIRNLIVSEIFHDTDDSKFIFDYIALLDDLINTEK 365

Query: 385 DVSILVKEKIISNTSGCSDQQVAELINNLRLNAPTFPDKCYYYNHMSKELNDYCNKWWHR 444
           DVSILVKEKI++N  G SD+ V +LIN+LRLNAPTFP   YYY++MSK+LN++C KWW+R
Sbjct: 366 DVSILVKEKILTNEIGGSDEPVYKLINDLRLNAPTFP-LAYYYSNMSKDLNEHCRKWWNR 425

Query: 445 SMASLRRDYFNTPWASISFFAATFLLILTFLQTLFSTPFIFY 471
           S+ASLRRDYFN PWASISF AAT LLILT LQTLFS P  F+
Sbjct: 426 SLASLRRDYFNNPWASISFVAATVLLILTLLQTLFSAPAFFH 463

BLAST of Bhi09G001071 vs. ExPASy TrEMBL
Match: A0A6J1BVD4 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111005065 PE=4 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 2.2e-93
Identity = 203/435 (46.67%), Postives = 281/435 (64.60%), Query Frame = 0

Query: 44  EEGD-DVVISIEKMIMELPSVNPNS-SLYKVPKGHIKTNETMYTPRFISIGPFHHGQPEL 103
           EE D  + +SI K++ E P +      +Y+VPK       T YTP+ I+IGPFHHG+ +L
Sbjct: 44  EEDDLSLELSIIKLLEEQPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDL 103

Query: 104 MAAEQYKLWALKTYLCRAKMGVKEAMSLACSWEEKARSCYGHPIDMNKQNFAKMMLLDGC 163
           +A +Q KL   + YL R +  VK  +++A  WE KAR  Y  PI+M   +F +MML+D C
Sbjct: 104 LATQQSKLHCFRNYLIRIERDVKYVVAIARQWEIKARRLYAEPINMTSDDFVRMMLIDAC 163

Query: 164 FILEFLNITNDLYIEDDEKWIDDGSLIYRILYDVERDLTIYENQLPFFVLQQLYDLLPFP 223
           FI+E + I N    E  E +  D      IL+D+ ++LT+ ENQLPFFVLQ L+DL  FP
Sbjct: 164 FIVELMIILNFECFETREGF--DLLFAKVILHDLYQELTMLENQLPFFVLQALFDL--FP 223

Query: 224 PENDDPAVHFMECMSK-LFIATTTGYSY--HYNATTEVRHLVDLLKFYFIPSPDTDEYKK 283
           P  +   + F++   K L      GY+     ++T EV HLVDLL FY++PSPDT+EY++
Sbjct: 224 PHKNKANISFIQLTGKFLSNGLIRGYNLPCGVSSTEEVNHLVDLLGFYYVPSPDTEEYRQ 283

Query: 284 FKESEEESYLESPNLTKLCEAGVKVQKADEAKSMMDFSFKNGILKIPPTIIHNVFEIHIR 343
            KE +   +L  P +TKLCEAGVKV+KA  A+S++D SFK G+L+IPP  IH+ FEI++R
Sbjct: 284 NKEIKNRIFLLPPTITKLCEAGVKVKKAIGARSLLDISFKRGVLQIPPFEIHDDFEIYVR 343

Query: 344 NLIVSEIV----SEDETYLFHYVALLDDLIDTEKDVSILVKEKIISNTSGCSDQQVAELI 403
           NL+  E       +D+ Y+ HY+  LD LI T +DV++LVKE II N  G S+++V+EL 
Sbjct: 344 NLMAFEQYCVREPDDDRYVIHYIEFLDGLISTAEDVALLVKEGIIINHIGGSNKEVSELF 403

Query: 404 NNLRLNAPTFPDKCYYYNHMSKELNDYCNKWWHRSMASLRRDYFNTPWASISFFAATFLL 463
           NNL  N P  P + Y+Y   SK L+D+C KWW RS A+LRRDYF++PWA IS FAATFL+
Sbjct: 404 NNLCKNTP-IPFQFYFY-ATSKNLHDHCRKWWPRSKATLRRDYFHSPWAYISVFAATFLI 463

Query: 464 ILTFLQTLFSTPFIF 470
           +L  LQT+F+    F
Sbjct: 464 LLALLQTIFTAKSTF 472

BLAST of Bhi09G001071 vs. ExPASy TrEMBL
Match: A0A6J1BQT6 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111004764 PE=4 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 8.0e-88
Identity = 190/434 (43.78%), Postives = 270/434 (62.21%), Query Frame = 0

Query: 37  NNNNKDVEEGDDVVISIEKMIMEL--PSVNPNSSLYKVPKGHIKTNETMYTPRFISIGPF 96
           NNN  D +    VVISIE+M   L  P ++P  S+Y+VPK  +  N   YTP+ ISIGPF
Sbjct: 25  NNNEVDEQHCRHVVISIEEMCKRLPPPPIDPECSIYRVPKRLLNMNRKAYTPQVISIGPF 84

Query: 97  HH-GQPELMAAEQYKLWALKTYLCRAKMGVKEAMSLACSWEEKARSCYGHPIDMNKQNFA 156
           HH  Q  L+  +Q+KL AL +YL R KM V+  + +  +WE +ARSCYG PI MN   F 
Sbjct: 85  HHSNQSNLIVTQQHKLQALDSYLHRVKMTVEAVVKITQNWENRARSCYGEPIKMNNDKFV 144

Query: 157 KMMLLDGCFILEFLNITNDLYIEDDEKWIDDGSLIYRILYDVERDLTIYENQLPFFVLQQ 216
            M+LLDGCF++ FL +  + Y  D+  +  D S    +  D+  D+T+ ENQLPFFVLQ 
Sbjct: 145 TMLLLDGCFVVXFLILDYNNYETDENGF--DSSFYEAMSSDIYGDMTMLENQLPFFVLQG 204

Query: 217 LYDLLPFPPENDDPAVHFMECMSKLFIATTTGYSYHYNAT-TEVRHLVDLLKFYFIPSPD 276
           LYDL+P   E  + ++  ++ +   F  +   +    + +   V+HLVDLL  YF+P  D
Sbjct: 205 LYDLIPKDHEIKNNSL--IQLIETFFSRSMNNHEIPCHVSPPNVKHLVDLLSLYFLPPCD 264

Query: 277 TDEYKKFKESEEESYLESPNLTKLCEAGVKVQKADEAKSMMDFSFKNGILKIPPTIIHNV 336
           T +     + +++ YL +P +T+LCEAGV ++K  EA  +MD SFKNG+L+IPP  I + 
Sbjct: 265 TKQ-----QHDKDEYLVTPCVTELCEAGVTIKKGKEATCLMDISFKNGVLEIPPLDIDDH 324

Query: 337 FEIHIRNLIVSE--IVSEDETYLFHYVALLDDLIDTEKDVSILVKEKIISNTSGCSDQQV 396
           FE  +RNL+  E    +    Y   Y   LD +I TEKDV +LV+  II N+ G SD++V
Sbjct: 325 FETIVRNLMAFEHYPAANYHRYTIQYALFLDYMISTEKDVRLLVEAGIIINSIGGSDKEV 384

Query: 397 AELINNLRLNAPTFPDKCYYYNHMSKELNDYCNKWWHRSMASLRRDYFNTPWASISFFAA 456
           + L N+L     + P   +Y NH++K L+D+C KWW RS A+L+RDYFN+PWA IS  AA
Sbjct: 385 SRLFNDLG-KYVSIPGGVHYLNHITKPLHDHCKKWWPRSKATLKRDYFNSPWAFISIVAA 444

Query: 457 TFLLILTFLQTLFS 465
           T+++ILT LQT+F+
Sbjct: 445 TYIIILTLLQTIFT 448

BLAST of Bhi09G001071 vs. ExPASy TrEMBL
Match: A0A6J1BR71 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111005028 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 1.5e-78
Identity = 191/450 (42.44%), Postives = 262/450 (58.22%), Query Frame = 0

Query: 30  EEMIMENNNNNKDVEEGD----DVVISIEKMIMELPSVNPNSSLYKVPKGHIKTNETMYT 89
           E+  +E  + NK ++E +     V IS++ M+ +L  ++   S+Y+V K     N+  YT
Sbjct: 2   EDDHIETYDLNKKIDEVELEQPHVTISMKNMLEKLHPISEECSIYRVSKRLHNINDMAYT 61

Query: 90  PRFISIGPFHHGQPELMAAEQYKLWALKTYLCRAKMGVKEAMSLACSWEEKARSCYGHPI 149
           P+ ISIGPFHHGQ E MA EQ KL  L  YL R  MG+++A  +A  WE +AR CY   I
Sbjct: 62  PQAISIGPFHHGQKEFMAMEQLKLRFLDAYLRRVGMGIEDAFEIAQGWETRARKCYAEHI 121

Query: 150 DMNKQNFAKMMLLDGCFILEFLNITNDLYIEDDEKWIDDGSLIYRILYDVERDLTIYENQ 209
           DM   NF KMML+DG F++EF+ +              + +L   I  D+ RDL + ENQ
Sbjct: 122 DMKSDNFVKMMLVDGAFLVEFIRMHYQWATMTQPNL--NYTLFQAIHVDIYRDLILLENQ 181

Query: 210 LPFFVLQQLYDLLPFPPENDDPAVHFMECMSKLFIATTTGYSYHYNATTEVRHLVDLLKF 269
           LPFF+L+ L D       +  P V F     + +       S     T +  HLVD L F
Sbjct: 182 LPFFILECLLDKC----SSSTPFVLFTSTFCRWYTGARELISDKL-LTKKPNHLVDFLSF 241

Query: 270 YFIPSPDTDEYKKFKESEEESYLESPNLTKLCEAGVKVQKADEAKSM-MDFSFKNGILKI 329
           Y+     T +  K K ++ ES    P  T+L EAGV+ QKA E K + MD  FK+G+L I
Sbjct: 242 YYALPTVTGKNDKLKYNKRES---PPTATELWEAGVEFQKATEDKRLIMDIRFKDGVLSI 301

Query: 330 PPTIIHNVFEIHIRNLIVSE--IVSEDETYLFHYVALLDDLIDTEKDVSILVKEKIISNT 389
           P   IH+ FE ++RNL+  E   + +DE  L  YV  LD+LI TE+DVS+LVK  II+N 
Sbjct: 302 PHLEIHDAFETYVRNLLAYEHYHIGDDERCLIQYVYFLDELISTERDVSLLVKAGIITNN 361

Query: 390 SGCSDQQVAELINNLRLNAPTFPDKCYYYNHMSKELNDYCNKWWHRSMASLRRDYFNTPW 449
            G +++ V++L N+L  +     D  YYY  +S +L+ YC  WWHRSMASLRRDYFNTPW
Sbjct: 362 IGGNNEDVSKLFNDLCKDINISCD-FYYYADISMDLHKYCETWWHRSMASLRRDYFNTPW 421

Query: 450 ASISFFAATFLLILTFLQTLFSTPFIFYNK 473
           A ISF AATFL++LT +Q ++S   I Y+K
Sbjct: 422 AFISFLAATFLVLLTSMQAIYSA--ISYHK 438

BLAST of Bhi09G001071 vs. ExPASy TrEMBL
Match: A0A1S3BBL9 (UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103488293 PE=4 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 1.3e-77
Identity = 188/445 (42.25%), Postives = 267/445 (60.00%), Query Frame = 0

Query: 47  DDVVISIEKMIMELPSVNPNS-SLYKVPKGHIKTNETMYTPRFISIGPFH-HGQPELMAA 106
           D+VVISIEKM+ ++P  + N  S+Y+VPK   + N   Y P+ ISIGPFH H    L+A 
Sbjct: 39  DNVVISIEKMLDQVPPTHENQCSIYRVPKQLREMNPKAYAPQLISIGPFHYHTHKNLIAN 98

Query: 107 EQYKLWALKTYLCRA-KM----------GVKEAMSLACSWEEKARSCYGHPIDMNKQNFA 166
           EQYKL     YL R  KM           V++ +  A SW E+AR+CY   I+MN ++F 
Sbjct: 99  EQYKLQGFINYLRRVYKMESLEQLVRTKSVEDLVKRAQSWVEEARNCYAETINMNDEDFI 158

Query: 167 KMMLLDGCFILEFLNITNDLYIEDDEKWID------DGSLIYRILYDVERDLTIYENQLP 226
           KMML+DGCFI+EF  +  + Y E  E            S     + D++ DL   ENQLP
Sbjct: 159 KMMLVDGCFIVEFFILDFEEYNESHESLFPQIENNVSMSFYKERIPDIDEDLIKLENQLP 218

Query: 227 FFVLQQLYDLLPFPPENDDPAVHFMECMSKL-FIATTTGYSYHYN----ATTEVRHLVDL 286
           FFVLQ L+DL+  P   D P      C  +L +   T G+  +Y      + + +H +D 
Sbjct: 219 FFVLQHLFDLI--PKHKDAP-----NCFKQLTYEYLTMGWLENYEPSDILSIKPKHFIDF 278

Query: 287 LKFYFIPSPDTDEYKKFKESEEESYLESPNLTKLCEAGVKVQKADE-AKSMMDFSFKNGI 346
           L FY +P    +  +K  + EE + +  P++T++CEAGV ++KAD+  K +++  F+NGI
Sbjct: 279 LSFYLVPEHQYEHDQKSNDEEEWNIIIPPSITEICEAGVTIKKADKNTKCLLNIRFENGI 338

Query: 347 LKIPPTIIHNVFEIHIRNLIVSE--IVSEDETYLFHYVALLDDLIDTEKDVSILVKEKII 406
           L+IPP  I + FE  +RNL+  E   V    TY+  Y+  +D LI TEKDV++LVKEKII
Sbjct: 339 LEIPPLHIDDYFEPMMRNLLAFEHFPVEVKNTYVIPYLTFMDYLIITEKDVNLLVKEKII 398

Query: 407 SNTSGCSDQQVAELINNLRLNAPTFPDKCYYYNHMSKELNDYCNKWWHRSMASLRRDYFN 465
            N  G SD++V++L NNL     + P+   Y+N  SK L D+C++ W+++ ASL+ +YFN
Sbjct: 399 INDIGGSDREVSQLFNNLCKFVSSSPND-NYFNDTSKALRDHCDRRWNKAKASLKHNYFN 458