Bhi09G000962 (gene) Wax gourd (B227) v1

Overview
NameBhi09G000962
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionhyoscyamine 6-dioxygenase-like
Locationchr9: 28080393 .. 28087449 (+)
RNA-Seq ExpressionBhi09G000962
SyntenyBhi09G000962
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTAATTAGATTTCATTCTCTTTAAGTTACCGCTACCTTCAATTCATCTCAAACATAGTTTCCCATGAGGTAAGGTGTTACGAAATTTTGGTTAAATAAAAATAATAATTTTATGGAAGAAAAGATGTCACAAAATTTATTAATAGATACTAAATTCTGTTTAGTAAAAATTAAGGATAAACTAATAATATTGACTATTTTTAAGATATATCAAAAATACAAGAATTAAATTCGAACATTTAAAAGTACGAAAAACTTAATTGAACAATTTCTAAAGTATATTAAATTAAAGATCAATATTTGTTGTGATTGAATATAACTTTATATTGCAAGAAACATGTTTATAATTTTTTTAAAAAAACCATAAAAAGTATAGAAAATGTATTTAAAATCCAAACCCTTGGAGAAAAAAAAAAAAAAAAAGAAAATGAATCAAATGAAAACAAACAAGTAGTGAAATAAATAAATATTAGGGCAAGTTGCAAGTTTTGAGGTTTGTATTAATATAAGTCTTCTGTTGCAGCAAGTTGCATATATCTATTTAATGCCATGAAGAAAAAGGGGGAGAAGCCAACTGAAAGAAGAAACCTCTCTACTATATAGAGCTTGAGTCCTTAAAAGTTTAGGACATTATCAAATCATCTATTGTGAGCAAAATTTTAAATAGTAAGTACAATATGGGAGATGCTAATAAGTTTGTAGCCAATTGGGAAGGTGTTAAATCGGTACCGGAAAGTTATGTGTATCCGCCGGGGAAGAGGCCCGGGAACGTCGTTGTTCCGATGGAGAACGCTATTCCGGTGATTGATCTCTCTATGCAAGACCGAAATCTTCTTATTCGCAAAATCCTCGATGCATCTAAAGAATTTGGATTTTTTCAGGTTTGAAAGCTACTCTCGTATATTATCTCTTGTCTTTTTCATCTTTTCCTTTCGTTTAAGGTATATAAAAATGTTTTATTTTTGTTTCTAAACTTTTGTGCAGAATTGAGTTATGAAAATGTTTCTTAATTTAAATTAAGTAAATCTTCAACAACCATTTATCAATAAATTTAAAAATGTTTGAATTTAAAATTTTAGTGACAAAAAGATTTTTGGTAAATAAAGTTCATGAATATGTCAATTCTGAAAATAGTTAATTAGTTTTGGTATAAAGTTTTACTGAAAAATTAGATGTTGGAATCTTCTTTTTCTTTTTCCTTTTTTTTTTTTTTTTTTTTGGCCTTTTTGTTTCTTCTTGCTTGGGAATATGTCCCTATTTCCCATTTGGGGTTCAAGTGTTTGGTATGAATATGCCTTGGAAGCTTAATTTTTTCCCAGTGTTGTCAAAGGAAAATTCTTATAAATAGAAAATTTTAAAAATATTTATATTTTATAATAAAATGTTTTAAAAATGTTTTGTATTTTCGAAATTTTTTTGTTAAATATTTTTAAATATTTTTTATATTTAAAAATAACTCGTTGTCAAATTATATTATATTACACATACATATATACATATAGATATATACATAGTTTCTATGAGCAACAAATATCTTTTATTAATCTTTGAAGTTTAGGTTCGTTAATTTTCTTTGGTATTCTTACTATCATCTTCACATTTCTTAAAAAAATATATTTTAAATTATTTCTAATCAGTTCAAAGAACAAAGCTATTTTTTTAATCTCGTTTGATGATGATTTCATCTTTAATTACATGTTTTTAAAATTTATGCTAGTTTTCTATCCATTTTTCAATTGTAATTTTTACGTTTCTTAATGGAACACAATTAAATTCTCAATCAACTCCTAAAATAAGAACTACTTTCCTTAGTTTCCAAATCTGATTTAGACTTTTGAAAACAATAGTAGAAAGTGGATAAGGAAACATATAAATCTATGGGTGGTAATAGCATTTCTAAGCTTAATTTTTAAATCAAATGATTACCAAACGAAACTAGTTTTTCAGAACCTCGCTTGATTGTTTTTTAAAACATTAATAGAAATGATGTAATAAACAAAGAAAACATATAGGTATATGAGTAGTTTGAGTCTCGTTTGGTAAACATGTAGTTTTTTGTTTTTTGTTTTTGAAAATTAAACTTATAGATACTACTTCCACCTCAAAATTTCTTTTTTTGTTAACTACTTTTTACCAATTGTTTAAAAAAACTACTAAAAAATTAGTTTTTATTTTTGGAATTTGACTAAGAATTAAGAAAGATGCAAATCATTGTAAGAAATAGATAGGAAATAGGCTTAATTTTCAAAAATCAAAACAAAAAAAAAAAATAGTTACCAAGCAAAACCTTATTTAATAAGCTTAGCTTTTCAAAAACAAAATGTTTATCAAACGAGATCTTAATATCTTAATGCGTGACTATCAAATTAAACAAATAATGTCAATGTATATAACCAATTAAAGGACATAGCTTGAAACATCAGTCATCACCATATTTACTCTTTCTTTCTTTTTTTTTTTTTTTAATTTCCTTTTGCAAGACAAAGAAACTCTCCATATTTTCTACCTTACTAAAGATTTCAATACTCTCCACTTTCAATAATCTAAATCACTTCCTATAATTTTACTTTTATATTCCATTAAAAATGTTTGATGTACACACCTATAGGAAGATCTTCATTTTTAACTATTGCAAGGAGTTAAATCACATCCCAAACATTATTTTAGGTGAAAGATGTCACATGAATATAATTTAATATAACCTAAAGATAGAAGAGAGAGAGAGGATGAGTTAACTTAACATGAGCATTAAAAGAGACATTAATAATGACAATAAACCAAAATGCCAACTCTATTTAAGATTCAAAAGTTGGCAGTGACATTGAATAATAATTAGTTCATAATTCTTAAATCCAAAGTGTTAAAAATGTGTTCTATTGAATAGGTCAATATTGTAAGTTTGTAGTCAACGATTAATTGAAAAGTAAAAATCGCTAAAGGCCTATTTGGTATGCAACTCAGATTCTGTTTTATGTTTTCATACCAACTCAGATTCTGTTTTATGTTTTTAAATTTACTGAATATAGTCAATATGTATTTGGTTGGCAGCCTGGATTCTGTTTTCGGATTCTGTGATTCAAACTGTGCAAATTCTAAAACTAACTTTTTTATGTTTTCAAAGTATCTTGATTTTGAATGCAAAGTTTTAAAATACAAAATTATATTAAAAAGAAAAAAAACAATAACAATTATAGTATTTCATGTTATAAACTCAACTATTTTTGTTAAATTAAAATATGTAATACATAATATATTATATGACATTACAAAATAATAATTATACAAAAATTTTGACAATAAGTTCATAAATTAATTAGTATTAGTTTATATTCAACCTAGTATTTAATAGGTAACTATAATTAGTTTTACAATTTATAAATATATTTTCAAACATATTTTAAAAATATGTTTAAATTAGAGTTTAATTTCTGAATGTGCTACCAAACATATATTTGAAAATATGAAATACAATTATGTTTTTTTTAATCCCTTGTTTTCAAGATTTTGTTTTCAAATTGTCTACCAAACAGACTCCAACTAGTTGAGAGTTATGTATATTGTATATCAATTTCAACTATTTTGATTCATTCTTCTATTGAAATATTTTGAATCTTTTATTAGGCGGTGTAAATGCAGCACAATATGTCTTGGTGGAGGCTCGTTGACCCAAAACCTAATTTATATTCATTTATGCATTATTTATGAGTTTGGCCCTAGGATTTAAAAGAGTGCACTAGATATAAACTCTCTAACCCTAAAGGCACCTTTACGACCTATATTTATAATATTCTCTCTAGTAATAAACTACTCTCTCCTTATGTCTGTGGATGTAATTAATACACTGTTTGTAATCTCTGTTTTCCTTACTTTCTTTAATTATCGAATTTCTTAACATCCTATTTTAGGTAATAAACCATGGAGTGTCGAAAACTGTATCCGGGGAGACGATGAGGATATTCAAGGAATTTCACGCGATGTCAGGGCCGGAGAAGGCGAAGGAATGCTCTAAAGACCCTAACAGAAGTTGCAGAGTTTACACAAGCAGTGAAAATTATATGAAGGAACAAATTCACTGTTGGAGGGATGCTTTGATTTTCAATTGTCATCCTTTGGAGAAATATGTTCATTTTTGGCCTCAAAATCCCCCCAAATACAGGTAAAAATAACACAAAATATTACACCTTCATTTAATGAACTAACTCAAATTTGAAGGAACAATTTTGATTATTTATCAATACTTTTTAGAAAACTTCTACTTGACATCTACCATATCTCACATTCTTAGAATGAAATAACTCAAATTATTTATTAATTTATAGAAATTTGTGTGCTTCATTTTAAAAAAATAGTTAGGTGGTTGGATAAGTGTTATAATGATGGTAGTGTATGATTTATTGTAGCTCTATATTAAAGATATAGTTAGATGTACTTTGTCATCCAATATATAGATCATGATTGATGTCTAGTGAGTTCGACTAACAGGAATTATGATCTATTCTAACAAATTATCGTTCACGTCAATTGATGTATCTTAAAAGTTACTTACATTTTTATAGGACTATTTTTAAATATAGAAAAATAAATCAAAATATTTACAAAATATAGCAAAATTTTAGAACTATCAATGATAGACGCTGATAGACTTCTATCCGTGTCTTCTATCATCGTCTATCATTGATAATTCAAAAAATTTTATATCTTGTAAATATTTTAAATAGTTTTACCATTTAAAATAATTTTCCTTTTTTATATGAATTATCAAATATATGGGTTTGGTTGCAAAGGTTGTTACTATAGACTTTTTTACAAGTTTGTGAATTTGTAAAATGAGTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGAAAAAGATATCCATTGACCATTATTAGATTTTTATTAAATATTTGTTTTATAAATAATAGAATGATTTGAAAACAGCCTCTCAACAAGTCTATAATTTTGATAATGTGAATCGATAGTTATAAAAAATAATTCAAGCTGACATAATGATTATAGATGAATCAACTATGTATAACAATAAATTGACCAATGATTTTTTGTTTGTTACTTGGTCATTGTTCCACATATATATAGATCAATGATTTATAGTAATTTAGTAAAATTAGTTATGTTATCCAACAAATTGTGGAGTGAAAAGATTCAAATTTCTGACATATGTATCATAACCAGTTGAGAGATGCTTGGTTGCGAACTTGAAAATACTTATTCATTTTAATATCCAAGTATGATATCTATGCTATGTTTTCCAACCTTATGACTATGACTAATGTAATAATTTTGGACTTATATTTTAGAGAGGTGGTTGGAGCGTATTGTGTCGCGATGAGAAAGTTGGTTTTGGAGATATTAGAGCTGATGAGTGAAGGGTTGGGGCTTGGAAAAGGGTACTTTGGAGGTGAAATGAGTGAAAATCCATTGTTGTTGGTAAACCATTACCCACCATGCCCAAACCCTAGCTTGACTTTGGGATTGAGCCAACATTGTGATCCAAGCCTCATCACTATTTTGTTTCAAGATGTCAATGGCCTTCAAGTCCTCAAGGATGGTCGATGGATTGGTGTTCAACCTATCGACAACGCCTTTGTGGTTAACATTGGCTTCGTATTGCAGGTACATTCTTCCATTGACGTTTAATTGCTGTTAGAAGATCAAGTAAAACGATTGAAGTGAGTTTGATATGACTTTTAAAAAAGTATAATAACTTTATGGTTTTAAAAAATCTAAAAAGTTTTTTTTTTTTTAAAAAAAAGGGTATAGATGCTTTTGGTGCATATTCTTTTATTTGCTTTAAGCCCTACCCCACTTTTTTCTTACCAATCTCTCTCTCTTACTAGTCTCTCATTATATAAACTCTCTAACGTTCACCTTTTTTTTAGTCTAATTATAATGCATTTCATAGTTTATATATACACACACTTACGGTTTAAATACTTTTTGGTTGATGTACTTTTGGCTTTGACTTATTTAGTTCCTATACTTTTGATATATTCATTTTGGTCCCTACTTTTAACTTTGGTATATTTTAATCTTTCTACTTTTAAAAGGTGAAAATTTTAATTTGATCACTTTCATTCTTAAGAGGACCAAAAAGGTTACTTTTTAAAGGTCAAATACCAAAATAAACAAAGTTGAAAATATAAGGATCAAAATGAAGATTTTGAAAGTATAGGGATCAAAATGAATCAAAATTAAAAGTATTGTGACAAAAATAAGCATTTTAAAAGTATAAGAACCAAAATGAACCAAATCTAATAGTACGGGGATGAAAATTGTTTTTAAACCTGCTTTTGATATTTTCATTCCAAAGATTTACTAGTACAAACAAAAAGATATTGAACTTTTTTTTCCCTTCATTTTGGTATTATTATACTTGAAAATGCATTTCACATAAACATTTCTTTTCAAAAAGCATTTCTAAGCACAGTCAAGCTAGTTCCATATAGAAAAGTGTTCTTCCATCACTACTTTGCTAAAACAACACTACTATTGGAAGCTTTACTAAGTATTTCATGGTTTTATGTAGGTAATTACCAATGGGAAACTAAAAGCAGCAGAACACCGAGCTGTGACGAATGCAAAGACATCTCGACAAAGCTTAACATATCTAGTTTATCCAAAAGATGAAGCGACTATGGAACCAGCAAAATGTATGATAAATGAAGCCAACCCTCGACGTTATCGTTCCCTTAACTTCAAAGATTTTCAAAGAAACTACCTACCCAGGGCTGTTGATACTAAGGCAGTAATGGAGTATATTGGCTCCAATCAGTCTTAAACTGTTTCTTTTCTCTGTTGGGTAGTGACTATTATCACAGTGTAAACAAGAAATAACATATAGTATAGTACCATCAAACAACACTTTACTACAAAACAACGTTTCACAAATCAAATCGACTTGAGATTACACTATTATAGTAAGACTTTTCTTATGTGACCATTAGTGTCTAGGCTCTTTTACATCGTAGAACAATCTACCGACATTTCGATGTCAAAAATCTAATTGGACTTTTACACTTTATAGGCAGCTGTTATGGAG

mRNA sequence

ATTTAATTAGATTTCATTCTCTTTAAGTTACCGCTACCTTCAATTCATCTCAAACATAGTTTCCCATGAGCAAGTTGCATATATCTATTTAATGCCATGAAGAAAAAGGGGGAGAAGCCAACTGAAAGAAGAAACCTCTCTACTATATAGAGCTTGAGTCCTTAAAAGTTTAGGACATTATCAAATCATCTATTGTGAGCAAAATTTTAAATAGTAAGTACAATATGGGAGATGCTAATAAGTTTGTAGCCAATTGGGAAGGTGTTAAATCGGTACCGGAAAGTTATGTGTATCCGCCGGGGAAGAGGCCCGGGAACGTCGTTGTTCCGATGGAGAACGCTATTCCGGTGATTGATCTCTCTATGCAAGACCGAAATCTTCTTATTCGCAAAATCCTCGATGCATCTAAAGAATTTGGATTTTTTCAGGTAATAAACCATGGAGTGTCGAAAACTGTATCCGGGGAGACGATGAGGATATTCAAGGAATTTCACGCGATGTCAGGGCCGGAGAAGGCGAAGGAATGCTCTAAAGACCCTAACAGAAGTTGCAGAGTTTACACAAGCAGTGAAAATTATATGAAGGAACAAATTCACTGTTGGAGGGATGCTTTGATTTTCAATTGTCATCCTTTGGAGAAATATGTTCATTTTTGGCCTCAAAATCCCCCCAAATACAGAGAGGTGGTTGGAGCGTATTGTGTCGCGATGAGAAAGTTGGTTTTGGAGATATTAGAGCTGATGAGTGAAGGGTTGGGGCTTGGAAAAGGGTACTTTGGAGGTGAAATGAGTGAAAATCCATTGTTGTTGGTAAACCATTACCCACCATGCCCAAACCCTAGCTTGACTTTGGGATTGAGCCAACATTGTGATCCAAGCCTCATCACTATTTTGTTTCAAGATGTCAATGGCCTTCAAGTCCTCAAGGATGGTCGATGGATTGGTGTTCAACCTATCGACAACGCCTTTGTGGTTAACATTGGCTTCGTATTGCAGGTAATTACCAATGGGAAACTAAAAGCAGCAGAACACCGAGCTGTGACGAATGCAAAGACATCTCGACAAAGCTTAACATATCTAGTTTATCCAAAAGATGAAGCGACTATGGAACCAGCAAAATGTATGATAAATGAAGCCAACCCTCGACGTTATCGTTCCCTTAACTTCAAAGATTTTCAAAGAAACTACCTACCCAGGGCTGTTGATACTAAGGCAGTAATGGAGTATATTGGCTCCAATCAGTCTTAAACTGTTTCTTTTCTCTGTTGGGTAGTGACTATTATCACAGTGTAAACAAGAAATAACATATAGTATAGTACCATCAAACAACACTTTACTACAAAACAACGTTTCACAAATCAAATCGACTTGAGATTACACTATTATAGTAAGACTTTTCTTATGTGACCATTAGTGTCTAGGCTCTTTTACATCGTAGAACAATCTACCGACATTTCGATGTCAAAAATCTAATTGGACTTTTACACTTTATAGGCAGCTGTTATGGAG

Coding sequence (CDS)

ATGGGAGATGCTAATAAGTTTGTAGCCAATTGGGAAGGTGTTAAATCGGTACCGGAAAGTTATGTGTATCCGCCGGGGAAGAGGCCCGGGAACGTCGTTGTTCCGATGGAGAACGCTATTCCGGTGATTGATCTCTCTATGCAAGACCGAAATCTTCTTATTCGCAAAATCCTCGATGCATCTAAAGAATTTGGATTTTTTCAGGTAATAAACCATGGAGTGTCGAAAACTGTATCCGGGGAGACGATGAGGATATTCAAGGAATTTCACGCGATGTCAGGGCCGGAGAAGGCGAAGGAATGCTCTAAAGACCCTAACAGAAGTTGCAGAGTTTACACAAGCAGTGAAAATTATATGAAGGAACAAATTCACTGTTGGAGGGATGCTTTGATTTTCAATTGTCATCCTTTGGAGAAATATGTTCATTTTTGGCCTCAAAATCCCCCCAAATACAGAGAGGTGGTTGGAGCGTATTGTGTCGCGATGAGAAAGTTGGTTTTGGAGATATTAGAGCTGATGAGTGAAGGGTTGGGGCTTGGAAAAGGGTACTTTGGAGGTGAAATGAGTGAAAATCCATTGTTGTTGGTAAACCATTACCCACCATGCCCAAACCCTAGCTTGACTTTGGGATTGAGCCAACATTGTGATCCAAGCCTCATCACTATTTTGTTTCAAGATGTCAATGGCCTTCAAGTCCTCAAGGATGGTCGATGGATTGGTGTTCAACCTATCGACAACGCCTTTGTGGTTAACATTGGCTTCGTATTGCAGGTAATTACCAATGGGAAACTAAAAGCAGCAGAACACCGAGCTGTGACGAATGCAAAGACATCTCGACAAAGCTTAACATATCTAGTTTATCCAAAAGATGAAGCGACTATGGAACCAGCAAAATGTATGATAAATGAAGCCAACCCTCGACGTTATCGTTCCCTTAACTTCAAAGATTTTCAAAGAAACTACCTACCCAGGGCTGTTGATACTAAGGCAGTAATGGAGTATATTGGCTCCAATCAGTCTTAA

Protein sequence

MGDANKFVANWEGVKSVPESYVYPPGKRPGNVVVPMENAIPVIDLSMQDRNLLIRKILDASKEFGFFQVINHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMKEQIHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGRWIGVQPIDNAFVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCMINEANPRRYRSLNFKDFQRNYLPRAVDTKAVMEYIGSNQS
Homology
BLAST of Bhi09G000962 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 256.9 bits (655), Expect = 2.3e-68
Identity = 140/330 (42.42%), Postives = 198/330 (60.00%), Query Frame = 0

Query: 16  SVPESYVYPPGKRPGNVVVPMENAIPVIDLSMQDRNLLIRKILDASKEFGFFQVINHGVS 75
           ++PE+YV P   RP    V      P+IDLS  DR+ LI++I  A   FGFFQVINHGV+
Sbjct: 14  TLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGVN 73

Query: 76  KTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMKEQIHCWRDALIFNCH 135
           K +  E + + +EF +MS  EK K  S DP ++ R+ TS  N  KE+++ WRD L  +C+
Sbjct: 74  KQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVNNWRDYLRLHCY 133

Query: 136 PLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYFGGEMSE-NPLL 195
           P+ KYV+ WP NPP ++E+V  Y   +R++  +I EL+SE LGL K Y    + E    +
Sbjct: 134 PIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQHM 193

Query: 196 LVNHYPPCPNPSLTLGLSQHCDPSLITILFQD--VNGLQVLKDGRWIGVQPIDNAFVVNI 255
            VN+YPPCP P LT GL  H DP+ +TIL QD  V GLQ+L DG+W  V P  +AFV+NI
Sbjct: 194 AVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVINI 253

Query: 256 GFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCM----INEANPRR 315
           G  LQ ++NG  K+  HRAVTN +  R S+   + P D A M PAK +     +E  P  
Sbjct: 254 GDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKP-V 313

Query: 316 YRSLNFKDFQRNYLPRAVDTKAVMEYIGSN 339
           Y+   + ++ + +  R +D +  +E   +N
Sbjct: 314 YKDFTYAEYYKKFWSRNLDQEHCLENFLNN 341

BLAST of Bhi09G000962 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 227.3 bits (578), Expect = 1.9e-59
Identity = 126/328 (38.41%), Postives = 188/328 (57.32%), Query Frame = 0

Query: 14  VKSVPESYVYPPGKRPG-NVVVPMENAIPVI---DLSMQDRNLLIRKILDASKEFGFFQV 73
           V  VP +YV P   RP  + V    ++IP+I   DL   +R  +I +   A    GFFQ+
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74

Query: 74  INHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMKEQIHCWRDA 133
            NHGV +    + M   +EF   S  E+ K  S D  ++ R+ TS  N  KE++  WRD 
Sbjct: 75  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDF 134

Query: 134 LIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYFGGEMS 193
           L  +C+P+E +++ WP  P  +REV   Y  ++R LVL +LE +SE LGL K      + 
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194

Query: 194 EN-PLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGRWIGVQPIDNA 253
           ++   + +N+YP CP P LT GL  H D +LIT+L QD V+GLQV KDG+WI V P+ N 
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254

Query: 254 FVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCMINEA--N 313
           F+VN+G  +QVI+N K K+  HRAV N+   R S+     P ++A + PA+ +INE   +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314

Query: 314 PRRYRSLNFKDFQRNYLPRAVDTKAVME 334
           P  YR+  + ++   +   A DT++ ++
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTESCID 341

BLAST of Bhi09G000962 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 224.9 bits (572), Expect = 9.5e-59
Identity = 120/333 (36.04%), Postives = 191/333 (57.36%), Query Frame = 0

Query: 8   VANWEGVKSVPESYVYPPGKRPG-NVVVPMENAIPVI---DLSMQDRNLLIRKILDASKE 67
           V+++     +P +YV P   RP  + V    ++IP+I   DL   +R ++++++  A   
Sbjct: 11  VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACST 70

Query: 68  FGFFQVINHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMKEQI 127
           +GFFQ+ NHGV  T   +   + +EF      E+ K  S DP ++ R+ TS  N   +++
Sbjct: 71  YGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGADKV 130

Query: 128 HCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGY 187
             WRD L  +C P+E ++  WP +P  +REV   Y  ++R LVL +LE +SE LGL   +
Sbjct: 131 LNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDH 190

Query: 188 FGGEMSENPL-LLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGRWIGV 247
               + ++   +  N+YPPCP P LT GL  H DP++IT+L QD V+GLQV KD +W+ V
Sbjct: 191 ISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAV 250

Query: 248 QPIDNAFVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCMI 307
            PI N F+VNIG  +QVI+N K K+  HRAV N +  R S+    +P  +A + PA  ++
Sbjct: 251 SPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELV 310

Query: 308 NEANPRR-YRSLNFKDFQRNYLPRAVDTKAVME 334
           NE +    YR+  F ++   +  R++ T + ++
Sbjct: 311 NEQDSLAIYRTYPFVEYWDKFWNRSLATASCLD 342

BLAST of Bhi09G000962 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 204.9 bits (520), Expect = 1.0e-52
Identity = 118/318 (37.11%), Postives = 173/318 (54.40%), Query Frame = 0

Query: 13  GVKSVPESYVYPPGKRP--GNVVVPMENAIPVIDLSMQD----RNLLIRKILDASKEFGF 72
           GV  VP+ YV PP +RP  G+ +   E  +PVIDLS+      R+L I +I  A KEFGF
Sbjct: 23  GVPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLHQPFLRSLAIHEISMACKEFGF 82

Query: 73  FQVINHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMKEQIHCW 132
           FQVINHG+  +V  + +    +F  +   EK    S + +   R Y +S N+  +++H W
Sbjct: 83  FQVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVR-YGTSLNHSTDRVHYW 142

Query: 133 RDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYFGG 192
           RD +    HPL K++  WP NPP Y++ VG Y  A   L  +++E +SE LGL K Y   
Sbjct: 143 RDFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAISESLGLEKNYLQE 202

Query: 193 EMSE-NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLK-DGRWIGVQPI 252
           E+ E + ++ VN YP CP P + LG+  H D S +TIL Q   GLQ++  +  W+ V  I
Sbjct: 203 EIEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIMDCNKNWVCVPYI 262

Query: 253 DNAFVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCMINEA 312
           + A +V +G  ++V++NG  K+  HR   N +  R S   L        + PA  ++N  
Sbjct: 263 EGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLHKKISPAPKLVNPN 322

Query: 313 NPRRYRSLNFKDFQRNYL 323
           N   Y   +F DF  NY+
Sbjct: 323 NAPAYGEFSFNDF-LNYI 338

BLAST of Bhi09G000962 vs. TAIR 10
Match: AT3G60290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 189.5 bits (480), Expect = 4.4e-48
Identity = 113/313 (36.10%), Postives = 158/313 (50.48%), Query Frame = 0

Query: 13  GVKSVPESYVYPPGKRP--GNVVVPMENAIPVIDLSMQDRNLL----IRKILDASKEFGF 72
           G   VP  Y+ PP +RP  G  +      +PVIDLS     LL    I +I  A K FGF
Sbjct: 23  GDPHVPTRYILPPSQRPMLGPSIGTSTINLPVIDLSFLHDPLLRLCVIHEIELACKGFGF 82

Query: 73  FQVINHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMKEQIHCW 132
           FQVINHG+S  V  +       F  +   EK    S +     R Y +S N+  +++H W
Sbjct: 83  FQVINHGISSAVVKDAQDSATRFFDLPADEKMHLVSDNFQEPVR-YGTSINHSTDRVHYW 142

Query: 133 RDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYFGG 192
           RD +    HPL  +++ WP NPP Y+E VG Y  A   L  +++E +SE LGL K Y   
Sbjct: 143 RDFIKHYSHPLSNWINLWPSNPPCYKEKVGKYAEATHVLHKQLIEAISESLGLEKNYLQE 202

Query: 193 EMSE-NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLK-DGRWIGVQPI 252
           E+ E + ++ VN YP CP P + LG+  H D   +TIL Q   GLQ+   +  W+ V  I
Sbjct: 203 EIEEGSQVMAVNCYPACPEPEIALGMPPHSDYGSLTILLQSSEGLQIKDCNNNWVCVPYI 262

Query: 253 DNAFVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCMINEA 312
           + A +V +G  ++V++NG  K+  HR   N    R S   L        + PA  ++NE 
Sbjct: 263 EGALIVQLGDQVEVMSNGIYKSVVHRVTVNKDYKRLSFASLHSLPMHKKISPATQLVNEN 322

Query: 313 NPRRYRSLNFKDF 318
            P  Y   +F DF
Sbjct: 323 KPAAYGEFSFNDF 334

BLAST of Bhi09G000962 vs. ExPASy Swiss-Prot
Match: P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.1e-70
Identity = 144/327 (44.04%), Postives = 201/327 (61.47%), Query Frame = 0

Query: 7   FVANWEGVKSVPESYVYPPGKRPGNVVVPMENAIPVIDLSMQDRNLLIRKILDASKEFGF 66
           FV+NW   KSV ES++ P  KR     VP+ N +P+IDL  Q  +LL+++I  A ++FG 
Sbjct: 4   FVSNW-STKSVSESFIAPLQKR-AEKDVPVGNDVPIIDL-QQHHHLLVQQITKACQDFGL 63

Query: 67  FQVINHGVSKTVSGETMRIFKEFHAMSGPEKAK-----ECSK---DPNRSCRVYTSSENY 126
           FQVINHG  + +  ETM + KEF A+   EK K     E +K      +  ++Y   E  
Sbjct: 64  FQVINHGFPEELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGEQL 123

Query: 127 MKEQIHCWRDALIFNCHPLEK-YVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGL 186
             E+   W+D L   CHPL++  V+ WP+ P KYREVV  Y V +RKL + +L+ + EGL
Sbjct: 124 SNEEFLYWKDTLAHGCHPLDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYICEGL 183

Query: 187 GLGKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQ--VLKD 246
           GL  GYF  E+S+  ++L N+YPPCP+PS TLG   H D +LIT+L QD+ GLQ  ++KD
Sbjct: 184 GLKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLIVKD 243

Query: 247 GRWIGVQPIDNAFVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATME 306
             WI VQPI  AFVVN+G  L+VITN K + + HR VT+    R S+  L+ P    T+E
Sbjct: 244 ATWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSCTIE 303

Query: 307 PAKCMINEANPRRYRSLNFKDFQRNYL 323
           PAK ++N+ NP  Y+  ++ +F   YL
Sbjct: 304 PAKELLNQDNPPLYKPYSYSEFADIYL 327

BLAST of Bhi09G000962 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 3.2e-67
Identity = 140/330 (42.42%), Postives = 198/330 (60.00%), Query Frame = 0

Query: 16  SVPESYVYPPGKRPGNVVVPMENAIPVIDLSMQDRNLLIRKILDASKEFGFFQVINHGVS 75
           ++PE+YV P   RP    V      P+IDLS  DR+ LI++I  A   FGFFQVINHGV+
Sbjct: 14  TLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGVN 73

Query: 76  KTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMKEQIHCWRDALIFNCH 135
           K +  E + + +EF +MS  EK K  S DP ++ R+ TS  N  KE+++ WRD L  +C+
Sbjct: 74  KQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVNNWRDYLRLHCY 133

Query: 136 PLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYFGGEMSE-NPLL 195
           P+ KYV+ WP NPP ++E+V  Y   +R++  +I EL+SE LGL K Y    + E    +
Sbjct: 134 PIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQHM 193

Query: 196 LVNHYPPCPNPSLTLGLSQHCDPSLITILFQD--VNGLQVLKDGRWIGVQPIDNAFVVNI 255
            VN+YPPCP P LT GL  H DP+ +TIL QD  V GLQ+L DG+W  V P  +AFV+NI
Sbjct: 194 AVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVINI 253

Query: 256 GFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCM----INEANPRR 315
           G  LQ ++NG  K+  HRAVTN +  R S+   + P D A M PAK +     +E  P  
Sbjct: 254 GDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKP-V 313

Query: 316 YRSLNFKDFQRNYLPRAVDTKAVMEYIGSN 339
           Y+   + ++ + +  R +D +  +E   +N
Sbjct: 314 YKDFTYAEYYKKFWSRNLDQEHCLENFLNN 341

BLAST of Bhi09G000962 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.2e-66
Identity = 127/321 (39.56%), Postives = 187/321 (58.26%), Query Frame = 0

Query: 16  SVPESYVYPPGKRPGNVVVPMENAIPVIDLSMQDRNLLIRKILDASKEFGFFQVINHGVS 75
           ++P  YV P  +RP   +V  +  IPV+DL+  DR  ++  + DA +  GFFQV+NHG+ 
Sbjct: 19  TMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGID 78

Query: 76  KTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMKEQIHCWRDALIFNCH 135
             +    M + +EF  +   EKAK  S DP +  R+ TS  N  KE +H WRD L  +C+
Sbjct: 79  AALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRLSTSF-NVRKETVHNWRDYLRLHCY 138

Query: 136 PLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYFGGEMSENPL-L 195
           PL ++V  WP NPP ++E++G YC  +R+L   + E +SE LGL  GY    + E    +
Sbjct: 139 PLHQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQEQHM 198

Query: 196 LVNHYPPCPNPSLTLGLSQHCDPSLITILFQD--VNGLQVLKDGRWIGVQPIDNAFVVNI 255
            VN+YP CP P LT GL  H DP+ +TIL  D  V GLQVL DG+WI V P   A V+NI
Sbjct: 199 AVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVINI 258

Query: 256 GFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCMINEANPRRYRSL 315
           G  LQ ++NGK ++  HRAV N+   R S+   + P +   + PAK +I + +P  YR+ 
Sbjct: 259 GDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVYRNY 318

Query: 316 NFKDFQRNYLPRAVDTKAVME 334
            + ++ + +  R +D +  +E
Sbjct: 319 TYDEYYKKFWSRNLDQEHCLE 338

BLAST of Bhi09G000962 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.7e-58
Identity = 126/328 (38.41%), Postives = 188/328 (57.32%), Query Frame = 0

Query: 14  VKSVPESYVYPPGKRPG-NVVVPMENAIPVI---DLSMQDRNLLIRKILDASKEFGFFQV 73
           V  VP +YV P   RP  + V    ++IP+I   DL   +R  +I +   A    GFFQ+
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74

Query: 74  INHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMKEQIHCWRDA 133
            NHGV +    + M   +EF   S  E+ K  S D  ++ R+ TS  N  KE++  WRD 
Sbjct: 75  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDF 134

Query: 134 LIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYFGGEMS 193
           L  +C+P+E +++ WP  P  +REV   Y  ++R LVL +LE +SE LGL K      + 
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194

Query: 194 EN-PLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGRWIGVQPIDNA 253
           ++   + +N+YP CP P LT GL  H D +LIT+L QD V+GLQV KDG+WI V P+ N 
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254

Query: 254 FVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCMINEA--N 313
           F+VN+G  +QVI+N K K+  HRAV N+   R S+     P ++A + PA+ +INE   +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314

Query: 314 PRRYRSLNFKDFQRNYLPRAVDTKAVME 334
           P  YR+  + ++   +   A DT++ ++
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTESCID 341

BLAST of Bhi09G000962 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.3e-57
Identity = 120/333 (36.04%), Postives = 191/333 (57.36%), Query Frame = 0

Query: 8   VANWEGVKSVPESYVYPPGKRPG-NVVVPMENAIPVI---DLSMQDRNLLIRKILDASKE 67
           V+++     +P +YV P   RP  + V    ++IP+I   DL   +R ++++++  A   
Sbjct: 11  VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACST 70

Query: 68  FGFFQVINHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMKEQI 127
           +GFFQ+ NHGV  T   +   + +EF      E+ K  S DP ++ R+ TS  N   +++
Sbjct: 71  YGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGADKV 130

Query: 128 HCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGY 187
             WRD L  +C P+E ++  WP +P  +REV   Y  ++R LVL +LE +SE LGL   +
Sbjct: 131 LNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDH 190

Query: 188 FGGEMSENPL-LLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGRWIGV 247
               + ++   +  N+YPPCP P LT GL  H DP++IT+L QD V+GLQV KD +W+ V
Sbjct: 191 ISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAV 250

Query: 248 QPIDNAFVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCMI 307
            PI N F+VNIG  +QVI+N K K+  HRAV N +  R S+    +P  +A + PA  ++
Sbjct: 251 SPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELV 310

Query: 308 NEANPRR-YRSLNFKDFQRNYLPRAVDTKAVME 334
           NE +    YR+  F ++   +  R++ T + ++
Sbjct: 311 NEQDSLAIYRTYPFVEYWDKFWNRSLATASCLD 342

BLAST of Bhi09G000962 vs. ExPASy TrEMBL
Match: A0A0A0K5H3 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378370 PE=3 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 8.1e-183
Identity = 308/335 (91.94%), Postives = 323/335 (96.42%), Query Frame = 0

Query: 6   KFVANWEGVKSVPESYVYPPGKRPGNVVVPMENAIPVIDLSMQDRNLLIRKILDASKEFG 65
           KFV+NWE V+SVPESYVYPP KRPGN+VVPM  AIPVIDLS++DR LL+RKILDASKEFG
Sbjct: 7   KFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFG 66

Query: 66  FFQVINHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMKEQIHC 125
           FFQ+INHGVSK VS ETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENY KEQ+HC
Sbjct: 67  FFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHC 126

Query: 126 WRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYFG 185
           WRDALIFNCHPLEKYVHFWPQ PPKYREVVGAYC+AMRKLVLEILELMSEGLGLGKGYFG
Sbjct: 127 WRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFG 186

Query: 186 GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGRWIGVQPID 245
           GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDG+WIGVQPID
Sbjct: 187 GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPID 246

Query: 246 NAFVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCMINEAN 305
           NAFVVNIGFVLQ+ITNGKL+AAEHRAVTN+KTSRQSLTYLVYPKDEA MEPAKCMINEAN
Sbjct: 247 NAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEAN 306

Query: 306 PRRYRSLNFKDFQRNYLPRAVDTKAVMEYIGSNQS 341
           P RYRSLNFKDFQRNYLPRAVDTKAVM+YI +NQS
Sbjct: 307 PPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of Bhi09G000962 vs. ExPASy TrEMBL
Match: A0A1S3BR20 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 2.9e-180
Identity = 310/339 (91.45%), Postives = 321/339 (94.69%), Query Frame = 0

Query: 2   GDANKFVANWEGVKSVPESYVYPPGKRPGNVVVPMENAIPVIDLSMQDRNLLIRKILDAS 61
           GDA KFV+NWE  +SVPESYVYPP KRPGNVVVPM  AIPVIDLS+ DR LL+RKIL AS
Sbjct: 4   GDA-KFVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILGAS 63

Query: 62  KEFGFFQVINHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMKE 121
           KE GFFQ+INHGVSK VS  TMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENY KE
Sbjct: 64  KESGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYKKE 123

Query: 122 QIHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGK 181
           QIHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGK
Sbjct: 124 QIHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGK 183

Query: 182 GYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGRWIGV 241
           GY GGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDG+WIGV
Sbjct: 184 GYLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGV 243

Query: 242 QPIDNAFVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCMI 301
           QPID AFVVNIGFVLQ+ITNGKL+AAEHRAVTN+KTSRQSLTYLVYPKDEATMEPAKCMI
Sbjct: 244 QPIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKCMI 303

Query: 302 NEANPRRYRSLNFKDFQRNYLPRAVDTKAVMEYIGSNQS 341
           NEANP RYRSLNFKDFQRNYLPRAVDTKAVM+YI +NQS
Sbjct: 304 NEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of Bhi09G000962 vs. ExPASy TrEMBL
Match: A0A6J1KW36 (hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 4.2e-171
Identity = 292/340 (85.88%), Postives = 312/340 (91.76%), Query Frame = 0

Query: 1   MGDANKFVANWEGVKSVPESYVYPPGKRPGNVVVPMENAIPVIDLSMQDRNLLIRKILDA 60
           MG+  KFV+NWEGV+SVP  Y+YPP KRPGNVVVPM NAIPV+DLS +DR LL+RKILDA
Sbjct: 1   MGEV-KFVSNWEGVQSVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDA 60

Query: 61  SKEFGFFQVINHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMK 120
           S+EFGFFQVINHGVS+ V  ET RIFK+FHAMSG EKAKECS+DP +SCRVYTSSENY K
Sbjct: 61  SQEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCRVYTSSENYRK 120

Query: 121 EQIHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLG 180
           EQ HCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMR LVLEILEL+SEGLGLG
Sbjct: 121 EQTHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLGLG 180

Query: 181 KGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGRWIG 240
           +GY   EMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDG+WIG
Sbjct: 181 RGYLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIG 240

Query: 241 VQPIDNAFVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCM 300
           V PIDNAFVVNIGFVLQVI+NGKLKAAEHRAVTNAK SRQSLTYLVYPKDE TMEPAKC+
Sbjct: 241 VHPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCL 300

Query: 301 INEANPRRYRSLNFKDFQRNYLPRAVDTKAVMEYIGSNQS 341
           INEANP RYR+L FKDFQRNYLPRA DTKAVME IGSN S
Sbjct: 301 INEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSNCS 339

BLAST of Bhi09G000962 vs. ExPASy TrEMBL
Match: A0A6J1H5H6 (hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=3 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.1e-170
Identity = 289/339 (85.25%), Postives = 311/339 (91.74%), Query Frame = 0

Query: 1   MGDANKFVANWEGVKSVPESYVYPPGKRPGNVVVPMENAIPVIDLSMQDRNLLIRKILDA 60
           MG+  KFV+NWEGV+SVP  YVYPP KRPGNVVVPM NAIPV+DLS  DR LL+RKILDA
Sbjct: 1   MGEV-KFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDA 60

Query: 61  SKEFGFFQVINHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMK 120
           S+EFGFFQVINHGVS+ V  ET RIFK+FHAMSG EK KECS+DPN+SCRVYTSSENY K
Sbjct: 61  SQEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRK 120

Query: 121 EQIHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLG 180
           EQ HCWRDALIFNCHPL+KYVHFWPQNPPKYREVVGAYC+AMR LVLEILEL+SEGLGLG
Sbjct: 121 EQTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLG 180

Query: 181 KGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGRWIG 240
           +GY GGEMSENPL+LVNHYP CPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDG+WIG
Sbjct: 181 RGYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIG 240

Query: 241 VQPIDNAFVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCM 300
           V PIDNAFVVNIGFVLQVI+NGKLKAAEHR VTNAK SRQSLTYLVYPKDE TMEPAKC+
Sbjct: 241 VHPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCL 300

Query: 301 INEANPRRYRSLNFKDFQRNYLPRAVDTKAVMEYIGSNQ 340
           INEANP RYR+L FKDFQRNYLPRA DTKAVME IGS++
Sbjct: 301 INEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338

BLAST of Bhi09G000962 vs. ExPASy TrEMBL
Match: A0A6J1CP90 (hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE=3 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 8.7e-169
Identity = 280/340 (82.35%), Postives = 308/340 (90.59%), Query Frame = 0

Query: 1   MGDANKFVANWEGVKSVPESYVYPPGKRPGNVVVPMENAIPVIDLSMQDRNLLIRKILDA 60
           MGD   FV+NW GV+SVPESYVYPP KRPGNV VP+ENAIPV+DL+  DR  L+ KILDA
Sbjct: 1   MGDQVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDA 60

Query: 61  SKEFGFFQVINHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMK 120
           S+EFGFFQVINHGVSKT+  ETMR+F+EFHAMSGPEK +ECSKDPNRSCRVYTSSEN++K
Sbjct: 61  SQEFGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVK 120

Query: 121 EQIHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLG 180
           E+ HCWRDALIFNCHPL+KYVHFWPQNP  YREVVGAYC+ MRKLV+EILEL+SEGLGLG
Sbjct: 121 EKTHCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLG 180

Query: 181 KGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGRWIG 240
           KGYFG EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ +NGLQVLKDG+WIG
Sbjct: 181 KGYFGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIG 240

Query: 241 VQPIDNAFVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCM 300
           V P+DNAFVVNIGFVLQV++NGKLKA EHRAV NAK SRQS+TYLVYPKD+ATMEPAKCM
Sbjct: 241 VHPVDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCM 300

Query: 301 INEANPRRYRSLNFKDFQRNYLPRAVDTKAVMEYIGSNQS 341
           INEANP  Y SL FKDFQRNYLP A DTKAV++YI S+ S
Sbjct: 301 INEANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS 340

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G24530.12.3e-6842.422-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G10490.11.9e-5938.412-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G10500.19.5e-5936.042-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G44800.11.0e-5237.112-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G60290.14.4e-4836.102-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
Match NameE-valueIdentityDescription
P243973.1e-7044.04Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1[more]
Q9FLV03.2e-6742.42Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S... [more]
Q8W2X51.2e-6639.56Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1... [more]
Q9ZSA72.7e-5838.41Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1[more]
Q9ZSA81.3e-5736.04Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K5H38.1e-18391.94Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G... [more]
A0A1S3BR202.9e-18091.45hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1[more]
A0A6J1KW364.2e-17185.88hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 ... [more]
A0A6J1H5H62.1e-17085.25hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=... [more]
A0A6J1CP908.7e-16982.35hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 40..146
e-value: 3.1E-18
score: 66.6
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 192..286
e-value: 6.3E-24
score: 84.4
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 6..327
e-value: 7.9E-88
score: 296.9
NoneNo IPR availablePANTHERPTHR47991:SF311-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASEcoord: 6..334
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 6..334
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 12..324
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 189..288
score: 12.820057

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi09M000962Bhi09M000962mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding