Bhi09G000953 (gene) Wax gourd (B227) v1

Overview


Name	Bhi09G000953
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	chr9: 27721831 .. 27722669 (-)
RNA-Seq Expression	Bhi09G000953
Synteny	Bhi09G000953

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAAAGTTTGTTTTGGAGTTATTGGATGTGATAAGTGAAGGGCTGAAGCTTGGAAGAGGATACTTAGGGGATGAAATGAGTTCAAATCCAACAATGCTTGTACATTATTATCCACCATGCCCAGACCCTAGCTTGACTTTGGGATTGAGGCAACATTGTGATGGAAGCCTCATCACCATCTTATTTCAAGATGTCAATGGCCTTCAAATCCTCAAGGATGGTGAATGGATTGCTGCTGATCCAATTGATGATGCCCTTTTGATTAACTTTGGTTATGTTCTTGAGGTATGAAACTAATACTAATAAATAAGAGAAAGTAGACCTATGCATGAGATCACTATATTTACACAATTAGTAAATTAGGGTGAGTTTCGTTTAGCTTTTCAAGTGTTTAATTTTGAAAAGAAATCATTTTGAAAGCAATTGAAGTATTTGACAACCACTCAAAATAGCTTTCGAAACATTTTCAAAACGCATTTTAAACAGTTTTTATAAAGACAGTTTAATTGAAAATGAATGTTTGGAATTTTTTTTTTCTCTTGTTAATCCAAATAGGCTCTTAGTCTGTGGTCTTACATTATTAGGTGATTAGCAATGGAAAGCTGAAGGCAGCAGAACATCGAGCAATGACAAATATGAACGTATCTCGACAGAGCTTAATCTACTTCGTCTGCCCTGAAAATGATGTGACTATAGAACCAGCAAAATGTCTGATAAATGAAACTAACCCTCTACGTTATCGCACCATCAAGTTTGAAGACTTCAGGGGAAAATATTATTCTGTGCTAGCTAATAAGGAGGAAATTATGAAATATATGAGCTTAAACAAAACTTAA

mRNA sequence

ATGAGAAAGTTTGTTTTGGAGTTATTGGATGTGATAAGTGAAGGGCTGAAGCTTGGAAGAGGATACTTAGGGGATGAAATGAGTTCAAATCCAACAATGCTTGTACATTATTATCCACCATGCCCAGACCCTAGCTTGACTTTGGGATTGAGGCAACATTGTGATGGAAGCCTCATCACCATCTTATTTCAAGATGTCAATGGCCTTCAAATCCTCAAGGATGGTGAATGGATTGCTGCTGATCCAATTGATGATGCCCTTTTGATTAACTTTGGTTATGTTCTTGAGGTGATTAGCAATGGAAAGCTGAAGGCAGCAGAACATCGAGCAATGACAAATATGAACGTATCTCGACAGAGCTTAATCTACTTCGTCTGCCCTGAAAATGATGTGACTATAGAACCAGCAAAATGTCTGATAAATGAAACTAACCCTCTACGTTATCGCACCATCAAGTTTGAAGACTTCAGGGGAAAATATTATTCTGTGCTAGCTAATAAGGAGGAAATTATGAAATATATGAGCTTAAACAAAACTTAA

Coding sequence (CDS)

ATGAGAAAGTTTGTTTTGGAGTTATTGGATGTGATAAGTGAAGGGCTGAAGCTTGGAAGAGGATACTTAGGGGATGAAATGAGTTCAAATCCAACAATGCTTGTACATTATTATCCACCATGCCCAGACCCTAGCTTGACTTTGGGATTGAGGCAACATTGTGATGGAAGCCTCATCACCATCTTATTTCAAGATGTCAATGGCCTTCAAATCCTCAAGGATGGTGAATGGATTGCTGCTGATCCAATTGATGATGCCCTTTTGATTAACTTTGGTTATGTTCTTGAGGTGATTAGCAATGGAAAGCTGAAGGCAGCAGAACATCGAGCAATGACAAATATGAACGTATCTCGACAGAGCTTAATCTACTTCGTCTGCCCTGAAAATGATGTGACTATAGAACCAGCAAAATGTCTGATAAATGAAACTAACCCTCTACGTTATCGCACCATCAAGTTTGAAGACTTCAGGGGAAAATATTATTCTGTGCTAGCTAATAAGGAGGAAATTATGAAATATATGAGCTTAAACAAAACTTAA

Protein sequence

MRKFVLELLDVISEGLKLGRGYLGDEMSSNPTMLVHYYPPCPDPSLTLGLRQHCDGSLITILFQDVNGLQILKDGEWIAADPIDDALLINFGYVLEVISNGKLKAAEHRAMTNMNVSRQSLIYFVCPENDVTIEPAKCLINETNPLRYRTIKFEDFRGKYYSVLANKEEIMKYMSLNKT

Homology

[Close]

BLAST of Bhi09G000953 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 137.9 bits (346), Expect = 8.0e-33
Identity = 70/172 (40.70%), Postives = 104/172 (60.47%), Query Frame = 0


Query: 1   MRKFVLELLDVISEGLKLGRGYLGDEMSSN-PTMLVHYYPPCPDPSLTLGLRQHCDGSLI 60
           +R  VL LL+ ISE L L +  + + +  +   M ++YYP CP P LT GL  H D +LI
Sbjct: 166 VRALVLTLLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPELTYGLPGHKDANLI 225

Query: 61  TILFQD-VNGLQILKDGEWIAADPIDDALLINFGYVLEVISNGKLKAAEHRAMTNMNVSR 120
           T+L QD V+GLQ+ KDG+WIA +P+ +  ++N G  ++VISN K K+  HRA+ N ++ R
Sbjct: 226 TVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMER 285

Query: 121 QSLIYFVCPENDVTIEPAKCLIN--ETNPLRYRTIKFEDFRGKYYSVLANKE 169
            S+  F CP  D  I PA+ LIN  E +P  YR   + ++  K++    + E
Sbjct: 286 ISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEYFEKFWDTAFDTE 337

[Close]

BLAST of Bhi09G000953 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 131.0 bits (328), Expect = 9.8e-31
Identity = 68/165 (41.21%), Postives = 99/165 (60.00%), Query Frame = 0


Query: 1   MRKFVLELLDVISEGLKLGRGYLGDEMSSNPT-MLVHYYPPCPDPSLTLGLRQHCDGSLI 60
           +R  VL LL+ ISE L L   ++ + +  +   M  +YYPPCP+P LT GL  H D ++I
Sbjct: 168 VRALVLRLLEAISESLGLESDHISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVI 227

Query: 61  TILFQD-VNGLQILKDGEWIAADPIDDALLINFGYVLEVISNGKLKAAEHRAMTNMNVSR 120
           T+L QD V+GLQ+ KD +W+A  PI +  ++N G  ++VISN K K+  HRA+ N    R
Sbjct: 228 TVLLQDQVSGLQVFKDDKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENER 287

Query: 121 QSLIYFVCPENDVTIEPAKCLINETNPLR-YRTIKFEDFRGKYYS 163
            S+  F  P  D  I PA  L+NE + L  YRT  F ++  K+++
Sbjct: 288 LSIPTFYFPSTDAVIGPAHELVNEQDSLAIYRTYPFVEYWDKFWN 332

[Close]

BLAST of Bhi09G000953 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 129.0 bits (323), Expect = 3.7e-30
Identity = 71/157 (45.22%), Postives = 92/157 (58.60%), Query Frame = 0


Query: 12  ISEGLKLGRGYLGDEMSSNPTMLV--HYYPPCPDPSLTLGLRQHCDGSLITILFQDVN-- 71
           IS GLK  R    +EM       V  +YYPPC  P L LGL  H DGS +T+L Q  N  
Sbjct: 195 ISLGLKEER---FEEMFGEAVQAVRMNYYPPCSSPDLVLGLSPHSDGSALTVLQQSKNSC 254

Query: 72  -GLQILKDGEWIAADPIDDALLINFGYVLEVISNGKLKAAEHRAMTNMNVSRQSLIYFVC 131
            GLQILKD  W+   P+ +AL+IN G  +EV+SNGK K+ EHRA+TN    R +++ F  
Sbjct: 255 VGLQILKDNTWVPVKPLPNALVINIGDTIEVLSNGKYKSVEHRAVTNREKERLTIVTFYA 314

Query: 132 PENDVTIEPAKCLI-NETNPLRYRTIKFEDFRGKYYS 163
           P  +V IEP   L+ +ETNP +YR+    D+   Y S
Sbjct: 315 PNYEVEIEPMSELVDDETNPCKYRSYNHGDYSYHYVS 348

[Close]

BLAST of Bhi09G000953 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 123.6 bits (309), Expect = 1.6e-28
Identity = 69/179 (38.55%), Postives = 105/179 (58.66%), Query Frame = 0


Query: 1   MRKFVLELLDVISEGLKLGRGYLGDEM-SSNPTMLVHYYPPCPDPSLTLGLRQHCDGSLI 60
           +R+   ++ ++ISE L L + Y+   +      M V+YYPPCP+P LT GL  H D + +
Sbjct: 159 VREVGFKIEELISESLGLEKDYMKKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNAL 218

Query: 61  TILFQD--VNGLQILKDGEWIAADPIDDALLINFGYVLEVISNGKLKAAEHRAMTNMNVS 120
           TIL QD  V GLQIL DG+W A +P  DA +IN G  L+ +SNG  K+  HRA+TN    
Sbjct: 219 TILLQDTTVCGLQILIDGQWFAVNPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENP 278

Query: 121 RQSLIYFVCPENDVTIEPAKCL----INETNPLRYRTIKFEDFRGKYYSVLANKEEIMK 173
           R S+  F+CP +   + PAK L     +ET P+ Y+   + ++  K++S   ++E  ++
Sbjct: 279 RLSVASFLCPADCAVMSPAKPLWEAEDDETKPV-YKDFTYAEYYKKFWSRNLDQEHCLE 336

[Close]

BLAST of Bhi09G000953 vs. TAIR 10
Match: AT1G12010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 122.9 bits (307), Expect = 2.7e-28
Identity = 65/160 (40.62%), Postives = 95/160 (59.38%), Query Frame = 0


Query: 7   ELLDVISEGLKLGRGYLGDEM--SSNPTML--VHYYPPCPDPSLTLGLRQHCDGSLITIL 66
           ELLD++ E L L +GYL      ++ PT    +  YPPCP P +  GLR H D   + +L
Sbjct: 130 ELLDLLCENLGLEKGYLKKVFHGTTGPTFATKLSNYPPCPKPEMIKGLRAHTDAGGLILL 189

Query: 67  FQD--VNGLQILKDGEWIAADPIDDALLINFGYVLEVISNGKLKAAEHRAMTNMNVSRQS 126
           FQD  V+GLQ+LKDG+W+   P+  +++IN G  LEVI+NGK K+  HR MT    +R S
Sbjct: 190 FQDDKVSGLQLLKDGDWVDVPPLKHSIVINLGDQLEVITNGKYKSVMHRVMTQKEGNRMS 249

Query: 127 LIYFVCPENDVTIEPAKCLINETNPLRYRTIKFEDFRGKY 161
           +  F  P +D  I PA  L+++ +  +Y +  F+D+   Y
Sbjct: 250 IASFYNPGSDAEISPATSLVDKDS--KYPSFVFDDYMKLY 287

[Close]

BLAST of Bhi09G000953 vs. ExPASy Swiss-Prot
Match: P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.7e-35
Identity = 77/179 (43.02%), Postives = 108/179 (60.34%), Query Frame = 0


Query: 1   MRKFVLELLDVISEGLKLGRGYLGDEMSSNPTMLVHYYPPCPDPSLTLGLRQHCDGSLIT 60
           +RK  + +LD I EGL L  GY  +E+S    ML +YYPPCPDPS TLG   H DG+LIT
Sbjct: 165 VRKLTMRMLDYICEGLGLKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLIT 224

Query: 61  ILFQDVNGLQ--ILKDGEWIAADPIDDALLINFGYVLEVISNGKLKAAEHRAMTNMNVSR 120
           +L QD+ GLQ  I+KD  WIA  PI  A ++N G  L+VI+N K + + HR +T+    R
Sbjct: 225 LLQQDLPGLQQLIVKDATWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDR 284

Query: 121 QSLIYFVCPENDVTIEPAKCLINETNPLRYRTIKFEDFRGKYYSVLANKEEIMKYMSLN 178
            S+   + P+   TIEPAK L+N+ NP  Y+   + +F   Y S  ++ +  +K   +N
Sbjct: 285 VSIATLIGPDYSCTIEPAKELLNQDNPPLYKPYSYSEFADIYLSDKSDYDSGVKPYKIN 343

[Close]

BLAST of Bhi09G000953 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.1e-31
Identity = 70/172 (40.70%), Postives = 104/172 (60.47%), Query Frame = 0


Query: 1   MRKFVLELLDVISEGLKLGRGYLGDEMSSN-PTMLVHYYPPCPDPSLTLGLRQHCDGSLI 60
           +R  VL LL+ ISE L L +  + + +  +   M ++YYP CP P LT GL  H D +LI
Sbjct: 166 VRALVLTLLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPELTYGLPGHKDANLI 225

Query: 61  TILFQD-VNGLQILKDGEWIAADPIDDALLINFGYVLEVISNGKLKAAEHRAMTNMNVSR 120
           T+L QD V+GLQ+ KDG+WIA +P+ +  ++N G  ++VISN K K+  HRA+ N ++ R
Sbjct: 226 TVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMER 285

Query: 121 QSLIYFVCPENDVTIEPAKCLIN--ETNPLRYRTIKFEDFRGKYYSVLANKE 169
            S+  F CP  D  I PA+ LIN  E +P  YR   + ++  K++    + E
Sbjct: 286 ISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEYFEKFWDTAFDTE 337

[Close]

BLAST of Bhi09G000953 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.1e-31
Identity = 70/175 (40.00%), Postives = 105/175 (60.00%), Query Frame = 0


Query: 1   MRKFVLELLDVISEGLKLGRGYLGDEMSSNPT-MLVHYYPPCPDPSLTLGLRQHCDGSLI 60
           +R+    L + ISE L L  GY+ + +      M V+YYP CP+P LT GL  H D + +
Sbjct: 164 VRELGFRLYEAISESLGLEGGYMRETLGEQEQHMAVNYYPQCPEPELTYGLPAHTDPNAL 223

Query: 61  TILFQD--VNGLQILKDGEWIAADPIDDALLINFGYVLEVISNGKLKAAEHRAMTNMNVS 120
           TIL  D  V GLQ+L DG+WIA +P   AL+IN G  L+ +SNGK ++  HRA+ N +  
Sbjct: 224 TILLMDDQVAGLQVLNDGKWIAVNPQPGALVINIGDQLQALSNGKYRSVWHRAVVNSDRE 283

Query: 121 RQSLIYFVCPENDVTIEPAKCLINETNPLRYRTIKFEDFRGKYYSVLANKEEIMK 173
           R S+  F+CP N V + PAK LI + +P  YR   ++++  K++S   ++E  ++
Sbjct: 284 RMSVASFLCPCNSVELGPAKKLITDDSPAVYRNYTYDEYYKKFWSRNLDQEHCLE 338

[Close]

BLAST of Bhi09G000953 vs. ExPASy Swiss-Prot
Match: Q40061 (Mugineic-acid 3-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS2 PE=2 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 2.5e-31
Identity = 69/161 (42.86%), Postives = 99/161 (61.49%), Query Frame = 0


Query: 2   RKFVLELLDVISEGLKLGRG-YLGDEMSSNPTMLVHYYPPCPDPSLTLGLRQHCDGSLIT 61
           R   +E+L ++ EGL L    ++GD       + ++YYPP P+PS TLGL  HCD  L+T
Sbjct: 156 RGVAMEILRLLCEGLGLRPDFFVGDISGGRVVVDINYYPPSPNPSRTLGLPPHCDRDLMT 215

Query: 62  ILFQD-VNGLQILKDGEWIAADPIDDALLINFGYVLEVISNGKLKAAEHRAMTNMNVSRQ 121
           +L    V GL+I   G WI   P+ ++L+INFG  LEV++NG LKA EHRA TN    R 
Sbjct: 216 VLLPGAVPGLEIAYKGGWIKVQPVPNSLVINFGLQLEVVTNGYLKAVEHRAATNFAEPRL 275

Query: 122 SLIYFVCPENDVTIEPAKCLINETNPLRYRTIKFEDFRGKY 161
           S+  F+ P +D  + PA+  ++E NP RYRT+   +F+ K+
Sbjct: 276 SVASFIVPADDCVVGPAEEFVSEDNPPRYRTLTVGEFKRKH 316

[Close]

BLAST of Bhi09G000953 vs. ExPASy Swiss-Prot
Match: Q40062 (2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=3)

HSP 1 Score: 135.6 bits (340), Expect = 5.6e-31
Identity = 67/156 (42.95%), Postives = 95/156 (60.90%), Query Frame = 0


Query: 7   ELLDVISEGLKLGRGYL-GDEMSSNPTMLVHYYPPCPDPSLTLGLRQHCDGSLITILFQD 66
           ELL ++ EG+ +   Y  GD    N  + +++YP CP+P   LG   HCD +LIT+L   
Sbjct: 162 ELLRLLCEGMGIQADYFEGDLSGGNVILNINHYPSCPNPDKALGQPPHCDRNLITLLLPG 221

Query: 67  -VNGLQILKDGEWIAADPIDDALLINFGYVLEVISNGKLKAAEHRAMTNMNVSRQSLIYF 126
            VNGL++   G+WI  DP  +A ++NFG  LEV++NG LK+ EHRAMTN  ++R S+  F
Sbjct: 222 AVNGLEVSYKGDWIKVDPAPNAFVVNFGQQLEVVTNGLLKSIEHRAMTNSALARTSVATF 281

Query: 127 VCPENDVTIEPAKCLINETNPLRYRTIKFEDFRGKY 161
           + P  +  I PAK  +++ NP  YRT  F DF   Y
Sbjct: 282 IMPTQECLIGPAKEFLSKENPPCYRTTMFRDFMRIY 317

[Close]

BLAST of Bhi09G000953 vs. ExPASy TrEMBL
Match: A0A6J1KW36 (hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 1.3e-62
Identity = 114/177 (64.41%), Postives = 144/177 (81.36%), Query Frame = 0


Query: 1   MRKFVLELLDVISEGLKLGRGYLGDEMSSNPTMLVHYYPPCPDPSLTLGLRQHCDGSLIT 60
           MR  VLE+L+++SEGL LGRGYL  EMS NP MLV++YPPCP+PSLTLGL QHCD SLIT
Sbjct: 161 MRTLVLEILELVSEGLGLGRGYLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLIT 220

Query: 61  ILFQDVNGLQILKDGEWIAADPIDDALLINFGYVLEVISNGKLKAAEHRAMTNMNVSRQS 120
           ILFQ+VNGLQ+LKDG+WI   PID+A ++N G+VL+VISNGKLKAAEHRA+TN  +SRQS
Sbjct: 221 ILFQEVNGLQVLKDGQWIGVHPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQS 280

Query: 121 LIYFVCPENDVTIEPAKCLINETNPLRYRTIKFEDFRGKYYSVLANKEEIMKYMSLN 178
           L Y V P+++VT+EPAKCLINE NP RYR +KF+DF+  Y    ++ + +M+ +  N
Sbjct: 281 LTYLVYPKDEVTMEPAKCLINEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSN 337

[Close]

BLAST of Bhi09G000953 vs. ExPASy TrEMBL
Match: A0A5D3DR20 (Hyoscyamine 6-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00110 PE=3 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 2.2e-62
Identity = 110/179 (61.45%), Postives = 146/179 (81.56%), Query Frame = 0


Query: 1   MRKFVLELLDVISEGLKLGRGYLGDEMSSNPTMLVHYYPPCPDPSLTLGLRQHCDGSLIT 60
           MRK VLE+L+++SEGL LG+GYLG EMS NP +LV++YPPCP+PSLTLGL QHCD SLIT
Sbjct: 80  MRKLVLEILELMSEGLGLGKGYLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLIT 139

Query: 61  ILFQDVNGLQILKDGEWIAADPIDDALLINFGYVLEVISNGKLKAAEHRAMTNMNVSRQS 120
           ILFQDVNGLQ+LKDG+WI   PID+A ++N G+VL++I+NGKL+AAEHRA+TN   SRQS
Sbjct: 140 ILFQDVNGLQVLKDGQWIGVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQS 199

Query: 121 LIYFVCPENDVTIEPAKCLINETNPLRYRTIKFEDFRGKYYSVLANKEEIMKYMSLNKT 180
           L Y V P+++ T+EPAKC+INE NP RYR++ F+DF+  Y     + + +M+Y+  N++
Sbjct: 200 LTYLVYPKDEATMEPAKCMINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 258

[Close]

BLAST of Bhi09G000953 vs. ExPASy TrEMBL
Match: A0A1S3BR20 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 3.7e-62
Identity = 110/179 (61.45%), Postives = 145/179 (81.01%), Query Frame = 0


Query: 1   MRKFVLELLDVISEGLKLGRGYLGDEMSSNPTMLVHYYPPCPDPSLTLGLRQHCDGSLIT 60
           MRK VLE+L+++SEGL LG+GYLG EMS NP +LV++YPPCP+PSLTLGL QHCD SLIT
Sbjct: 163 MRKLVLEILELMSEGLGLGKGYLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLIT 222

Query: 61  ILFQDVNGLQILKDGEWIAADPIDDALLINFGYVLEVISNGKLKAAEHRAMTNMNVSRQS 120
           ILFQDVNGLQ+LKDG+WI   PID A ++N G+VL++I+NGKL+AAEHRA+TN   SRQS
Sbjct: 223 ILFQDVNGLQVLKDGQWIGVQPIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQS 282

Query: 121 LIYFVCPENDVTIEPAKCLINETNPLRYRTIKFEDFRGKYYSVLANKEEIMKYMSLNKT 180
           L Y V P+++ T+EPAKC+INE NP RYR++ F+DF+  Y     + + +M+Y+  N++
Sbjct: 283 LTYLVYPKDEATMEPAKCMINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

[Close]

BLAST of Bhi09G000953 vs. ExPASy TrEMBL
Match: A0A6J1H5H6 (hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=3 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 1.9e-61
Identity = 112/172 (65.12%), Postives = 141/172 (81.98%), Query Frame = 0


Query: 1   MRKFVLELLDVISEGLKLGRGYLGDEMSSNPTMLVHYYPPCPDPSLTLGLRQHCDGSLIT 60
           MR  VLE+L+++SEGL LGRGYLG EMS NP MLV++YP CP+PSLTLGL QHCD SLIT
Sbjct: 161 MRTLVLEILELVSEGLGLGRGYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLIT 220

Query: 61  ILFQDVNGLQILKDGEWIAADPIDDALLINFGYVLEVISNGKLKAAEHRAMTNMNVSRQS 120
           ILFQ+VNGLQ+LKDG+WI   PID+A ++N G+VL+VISNGKLKAAEHR +TN  +SRQS
Sbjct: 221 ILFQEVNGLQVLKDGQWIGVHPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQS 280

Query: 121 LIYFVCPENDVTIEPAKCLINETNPLRYRTIKFEDFRGKYYSVLANKEEIMK 173
           L Y V P+++VT+EPAKCLINE NP RYR +KF+DF+  Y    ++ + +M+
Sbjct: 281 LTYLVYPKDEVTMEPAKCLINEANPPRYRALKFKDFQRNYLPRASDTKAVME 332

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BLAST of Bhi09G000953 vs. ExPASy TrEMBL
Match: A0A0A0K5H3 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378370 PE=3 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 3.2e-61
Identity = 108/179 (60.34%), Postives = 144/179 (80.45%), Query Frame = 0


Query: 1   MRKFVLELLDVISEGLKLGRGYLGDEMSSNPTMLVHYYPPCPDPSLTLGLRQHCDGSLIT 60
           MRK VLE+L+++SEGL LG+GY G EMS NP +LV++YPPCP+PSLTLGL QHCD SLIT
Sbjct: 163 MRKLVLEILELMSEGLGLGKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLIT 222

Query: 61  ILFQDVNGLQILKDGEWIAADPIDDALLINFGYVLEVISNGKLKAAEHRAMTNMNVSRQS 120
           ILFQDVNGLQ+LKDG+WI   PID+A ++N G+VL++I+NGKL+AAEHRA+TN   SRQS
Sbjct: 223 ILFQDVNGLQVLKDGQWIGVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQS 282

Query: 121 LIYFVCPENDVTIEPAKCLINETNPLRYRTIKFEDFRGKYYSVLANKEEIMKYMSLNKT 180
           L Y V P+++  +EPAKC+INE NP RYR++ F+DF+  Y     + + +M+Y+  N++
Sbjct: 283 LTYLVYPKDEAMMEPAKCMINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

The following BLAST results are available for this feature:

BLAST of Bhi09G000953 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10490.1	8.0e-33	40.70	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G10500.1	9.8e-31	41.21	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G21420.1	3.7e-30	45.22	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G24530.1	1.6e-28	38.55	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G12010.1	2.7e-28	40.63	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

BLAST of Bhi09G000953 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24397	1.7e-35	43.02	Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1	[more]
Q9ZSA7	1.1e-31	40.70	Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1	[more]
Q8W2X5	1.1e-31	40.00	Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1...	[more]
Q40061	2.5e-31	42.86	Mugineic-acid 3-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS2 PE=2 SV=1	[more]
Q40062	5.6e-31	42.95	2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=...	[more]

BLAST of Bhi09G000953 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KW36	1.3e-62	64.41	hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 ...	[more]
A0A5D3DR20	2.2e-62	61.45	Hyoscyamine 6-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3BR20	3.7e-62	61.45	hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1	[more]
A0A6J1H5H6	1.9e-61	65.12	hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=...	[more]
A0A0A0K5H3	3.2e-61	60.34	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 31..127 e-value: 1.0E-23 score: 83.7
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..178 e-value: 5.3E-52 score: 179.1
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 1..174
None	No IPR available	PANTHER	PTHR47991:SF31	1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE	coord: 1..174
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 1..163
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 29..127 score: 12.317982

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M000953	Bhi09M000953	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding