Bhi09G000951 (gene) Wax gourd (B227) v1

Overview


Name	Bhi09G000951
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	chr9: 27632262 .. 27634128 (-)
RNA-Seq Expression	Bhi09G000951
Synteny	Bhi09G000951

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAAGTTTTCTTGGGAGATAATCGATATGATAAGTGAAGGGTTGGGGCTTGAAAAAGGCTACTTGGAGGGAGAAATGAGTTCAAATCGAAGGATAACAGCAAACCATTATCCAATATGCCCAAACCCTAGCTTGACTTTGGGATTAAATCAACATTGTGATAGAAACCTCATTACCATCTTGTTTCAAGATGCCAATGGCCTTCAAGTCTTCAAGGATGGCCATTGGATTTCTTTTGATTCTGTTGATGATGCCTTTCTGATTAACTTTGGCTATCTTTTTGAGGTAAGTTGATATGCATTATTTCTTTAGAAAAAAAAAAAAAAAAAAAAAACCTTTTTCCTTCTCAATGTTCAATCCAGTTTTGATTTGGTTCTTAAGCTTCGAAATGTTACACTTTTGGTTCATAAGTTTTGTATTTGATTTCAATTTAGCCACTTGAAATTTGAGCTTGGTTTCAATCTGGTTCCTAAATTTTAAGATGTACACTTTTATCCTTGATTTTTTTATTAAATATTTGCCTAAGTCATTGGTGTTGTGTATATTCATTAATTGAAAATAAGTTTGAAGTGAAAGTTTAAAATTACTTTTATTAGTGATGACAAAAAGATGGGAGCAATTTGTAAATATAGCAATCAAGACCAAAATATTCACAGATATAGTACAATGCAGAAGAATTTGCAAATGTATCAAAATTTGACTCTTAGGGTCTATCAATGATTGATCATATCGTTAATAGAAGTCTATCTATGATAAAATCTGTCACTAATAGATTTTGCTATATTTTTAATTCTTTAAAAATGTTGCTATCAATTGAATTGTTAACACCAAAAGTGCTACCTATTGCAATTACCTAAGGGGTAGTTGCAACTATAGCAACTAAACCAAAAATGTTAGCAGATATAGCAAAATACAAAAGAATTTACGAATATAACAAAATTTAGATCCAATTCTAGGAATCTATCAAGGATAAACTATATCTCTAATAGGAGTCTGTCTATCACAGAGATAGAGTCTATCAACAATAGAATCTATATGTGGTATATTTTACTATATTTGTAATTTTTTATAAATATTACTATATATTTGATTATTAGTGCTAAAAGTGCTACTAATGTAATTACCTTTCTTTTTTTTTAACCAAATTTTGGATCATCCAAACCGACTTTCTCATACATAATATGGTTGATTATATATACAAATTTAAATTAATAGAAGGAATAAATGTATTTAACAGAAAGTTGATTAAGAAAAATAGAGACCAAATTAAGAGAAAAGATTTTGAACCTCCAACATTAGGTGGGTCCATGACAATTATATCATTGCATTATGTTCTCTTTACCAAAGTACTTAGATTATAAGTAGTCCATTAACAAAATGTTTAATAGTAGAATACCGTTGTTTTCGTTTAGAATTAATATTCCAAAGCGTGCTTTTAGTGTTTTTATTGTTATTAGAAGCTTCGTATATATATAAAAATAGTATTAATATAATCCCAACCGAGTCATAGTAAACACTTGAAAATACTTTTTGGTGAGAAATATTTATTGTAATATATATTGGAAGTTACAAGTAGAGTTGCTACCCATGAGATTATTTATGATCTATACAAATAGAGTTTGTGTGGTGTTATATAGGTGATTAGCAATGGAAAGCTCAAGGCAACCGAACATCGAGTCGTGACAAATGCAAAAGCATCTCGACAAAGCTTAGGATATTTTATTTTTCCTGAAAATGACGTGATAATAGAACCAGCAAAATGTCTGATAAACGAAGCCAATCCTGCACGTTATCGATCCCTCGAGTTGGAAGACTTCTATAGACATTTTTTTGCCGTGTCAACAAATACAGAGGAAACTATGAA

mRNA sequence

ATGGAAAAGTTTTCTTGGGAGATAATCGATATGATAAGTGAAGGGTTGGGGCTTGAAAAAGGCTACTTGGAGGGAGAAATGAGTTCAAATCGAAGGATAACAGCAAACCATTATCCAATATGCCCAAACCCTAGCTTGACTTTGGGATTAAATCAACATTGTGATAGAAACCTCATTACCATCTTGTTTCAAGATGCCAATGGCCTTCAAGTCTTCAAGGATGGCCATTGGATTTCTTTTGATTCTGTTGATGATGCCTTTCTGATTAACTTTGGCTATCTTTTTGAGGTGATTAGCAATGGAAAGCTCAAGGCAACCGAACATCGAGTCGTGACAAATGCAAAAGCATCTCGACAAAGCTTAGGATATTTTATTTTTCCTGAAAATGACGTGATAATAGAACCAGCAAAATGTCTGATAAACGAAGCCAATCCTGCACGTTATCGATCCCTCGAGTTGGAAGACTTCTATAGACATTTTTTTGCCGTGTCAACAAATACAGAGGAAACTATGAA

Coding sequence (CDS)

ATGGAAAAGTTTTCTTGGGAGATAATCGATATGATAAGTGAAGGGTTGGGGCTTGAAAAAGGCTACTTGGAGGGAGAAATGAGTTCAAATCGAAGGATAACAGCAAACCATTATCCAATATGCCCAAACCCTAGCTTGACTTTGGGATTAAATCAACATTGTGATAGAAACCTCATTACCATCTTGTTTCAAGATGCCAATGGCCTTCAAGTCTTCAAGGATGGCCATTGGATTTCTTTTGATTCTGTTGATGATGCCTTTCTGATTAACTTTGGCTATCTTTTTGAGGTGATTAGCAATGGAAAGCTCAAGGCAACCGAACATCGAGTCGTGACAAATGCAAAAGCATCTCGACAAAGCTTAGGATATTTTATTTTTCCTGAAAATGACGTGATAATAGAACCAGCAAAATGTCTGATAAACGAAGCCAATCCTGCACGTTATCGATCCCTCGAGTTGGAAGACTTCTATAGACATTTTTTTGCCGTGTCAACAAATACAGAGGAAACTATGAA

Protein sequence

MEKFSWEIIDMISEGLGLEKGYLEGEMSSNRRITANHYPICPNPSLTLGLNQHCDRNLITILFQDANGLQVFKDGHWISFDSVDDAFLINFGYLFEVISNGKLKATEHRVVTNAKASRQSLGYFIFPENDVIIEPAKCLINEANPARYRSLELEDFYRHFFAVSTNTEETM

Homology

[Close]

BLAST of Bhi09G000951 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 133.3 bits (334), Expect = 1.9e-31
Identity = 69/165 (41.82%), Postives = 99/165 (60.00%), Query Frame = 0


Query: 8   IIDMISEGLGLEKGYLEGEMSSN-RRITANHYPICPNPSLTLGLNQHCDRNLITILFQD- 67
           +++ ISE LGL K  +   +  + + +  N+YP CP P LT GL  H D NLIT+L QD 
Sbjct: 173 LLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDE 232

Query: 68  ANGLQVFKDGHWISFDSVDDAFLINFGYLFEVISNGKLKATEHRVVTNAKASRQSLGYFI 127
            +GLQVFKDG WI+ + V + F++N G   +VISN K K+  HR V N+   R S+  F 
Sbjct: 233 VSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFY 292

Query: 128 FPENDVIIEPAKCLINEA--NPARYRSLELEDFYRHFFAVSTNTE 169
            P  D +I PA+ LINE   +PA YR+    +++  F+  + +TE
Sbjct: 293 CPSEDAVISPAQELINEEEDSPAIYRNFTYAEYFEKFWDTAFDTE 337

[Close]

BLAST of Bhi09G000951 vs. TAIR 10
Match: AT1G05010.1 (ethylene-forming enzyme )

HSP 1 Score: 122.9 bits (307), Expect = 2.6e-28
Identity = 69/180 (38.33%), Postives = 98/180 (54.44%), Query Frame = 0


Query: 1   MEKFSWEIIDMISEGLGLEKGYLEGEMSSNRRIT----ANHYPICPNPSLTLGLNQHCDR 60
           +EK S E++D++ E LGLEKGYL+     ++R T     ++YP CPNP L  GL  H D 
Sbjct: 121 IEKLSEELLDLLCENLGLEKGYLKKVFYGSKRPTFGTKVSNYPPCPNPDLVKGLRAHTDA 180

Query: 61  NLITILFQD--ANGLQVFKDGHWISFDSVDDAFLINFGYLFEVISNGKLKATEHRVVTNA 120
             I +LFQD   +GLQ+ KDG W+    V  + ++N G   EVI+NGK K+ EHRV++  
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGEWVDVPPVKHSIVVNLGDQLEVITNGKYKSVEHRVLSQT 240

Query: 121 KA-SRQSLGYFIFPENDVIIEPAKCLI----NEANPARYRSLELEDFYRHFFAVSTNTEE 170
               R S+  F  P +D +I PA  LI     +     Y     ED+ + + AV    +E
Sbjct: 241 DGEGRMSIASFYNPGSDSVIFPAPELIGKEAEKEKKENYPRFVFEDYMKLYSAVKFQAKE 300

[Close]

BLAST of Bhi09G000951 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 3.3e-28
Identity = 63/163 (38.65%), Postives = 94/163 (57.67%), Query Frame = 0


Query: 8   IIDMISEGLGLEKGYLEGEMSSN-RRITANHYPICPNPSLTLGLNQHCDRNLITILFQD- 67
           +++ ISE LGLE  ++   +  + + +  N+YP CP P LT GL  H D  +IT+L QD 
Sbjct: 175 LLEAISESLGLESDHISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQ 234

Query: 68  ANGLQVFKDGHWISFDSVDDAFLINFGYLFEVISNGKLKATEHRVVTNAKASRQSLGYFI 127
            +GLQVFKD  W++   + + F++N G   +VISN K K+  HR V N +  R S+  F 
Sbjct: 235 VSGLQVFKDDKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFY 294

Query: 128 FPENDVIIEPAKCLINEANP-ARYRSLELEDFYRHFFAVSTNT 168
           FP  D +I PA  L+NE +  A YR+    +++  F+  S  T
Sbjct: 295 FPSTDAVIGPAHELVNEQDSLAIYRTYPFVEYWDKFWNRSLAT 337

[Close]

BLAST of Bhi09G000951 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 119.4 bits (298), Expect = 2.8e-27
Identity = 63/178 (35.39%), Postives = 99/178 (55.62%), Query Frame = 0


Query: 1   MEKFSWEIIDMISEGLGLEKGYLEGEM-SSNRRITANHYPICPNPSLTLGLNQHCDRNLI 60
           + +  ++I ++ISE LGLEK Y++  +    + +  N+YP CP P LT GL  H D N +
Sbjct: 159 VREVGFKIEELISESLGLEKDYMKKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNAL 218

Query: 61  TILFQDAN--GLQVFKDGHWISFDSVDDAFLINFGYLFEVISNGKLKATEHRVVTNAKAS 120
           TIL QD    GLQ+  DG W + +   DAF+IN G   + +SNG  K+  HR VTN +  
Sbjct: 219 TILLQDTTVCGLQILIDGQWFAVNPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENP 278

Query: 121 RQSLGYFIFPENDVIIEPAKCL----INEANPARYRSLELEDFYRHFFAVSTNTEETM 172
           R S+  F+ P +  ++ PAK L     +E  P  Y+     ++Y+ F++ + + E  +
Sbjct: 279 RLSVASFLCPADCAVMSPAKPLWEAEDDETKPV-YKDFTYAEYYKKFWSRNLDQEHCL 335

[Close]

BLAST of Bhi09G000951 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 119.0 bits (297), Expect = 3.7e-27
Identity = 61/161 (37.89%), Postives = 91/161 (56.52%), Query Frame = 0


Query: 1   MEKFSWEIIDMISEGLGLEKGYLEGEMSSNRR-ITANHYPICPNPSLTLGLNQHCDRNLI 60
           +EK ++ +++++S  LGL    L G  +     +  NHYP CPNP L LG+ +H D   +
Sbjct: 169 VEKLAFRLLELVSISLGLPGDRLTGFFNEQTSFLRFNHYPPCPNPELALGVGRHKDGGAL 228

Query: 61  TILFQDA-NGLQVFK--DGHWISFDSVDDAFLINFGYLFEVISNGKLKATEHRVVTNAKA 120
           T+L QD+  GLQV +  DG WI    + DA +IN G   +V +N +  + EHRVV N   
Sbjct: 229 TVLAQDSVGGLQVSRRSDGQWIPVKPISDALIINMGNCIQVWTNDEYWSAEHRVVVNTSK 288

Query: 121 SRQSLGYFIFPENDVIIEPAKCLINEANPARYRSLELEDFY 158
            R S+ +F FP ++  IEP + LI+E NP  Y+      F+
Sbjct: 289 ERFSIPFFFFPSHEANIEPLEELISEENPPCYKKYNWGKFF 329

[Close]

BLAST of Bhi09G000951 vs. ExPASy Swiss-Prot
Match: Q40062 (2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=3)

HSP 1 Score: 147.1 bits (370), Expect = 1.8e-34
Identity = 70/159 (44.03%), Postives = 99/159 (62.26%), Query Frame = 0


Query: 7   EIIDMISEGLGLEKGYLEGEMS-SNRRITANHYPICPNPSLTLGLNQHCDRNLITILFQD 66
           E++ ++ EG+G++  Y EG++S  N  +  NHYP CPNP   LG   HCDRNLIT+L   
Sbjct: 162 ELLRLLCEGMGIQADYFEGDLSGGNVILNINHYPSCPNPDKALGQPPHCDRNLITLLLPG 221

Query: 67  A-NGLQVFKDGHWISFDSVDDAFLINFGYLFEVISNGKLKATEHRVVTNAKASRQSLGYF 126
           A NGL+V   G WI  D   +AF++NFG   EV++NG LK+ EHR +TN+  +R S+  F
Sbjct: 222 AVNGLEVSYKGDWIKVDPAPNAFVVNFGQQLEVVTNGLLKSIEHRAMTNSALARTSVATF 281

Query: 127 IFPENDVIIEPAKCLINEANPARYRSLELEDFYRHFFAV 164
           I P  + +I PAK  +++ NP  YR+    DF R +  V
Sbjct: 282 IMPTQECLIGPAKEFLSKENPPCYRTTMFRDFMRIYNVV 320

[Close]

BLAST of Bhi09G000951 vs. ExPASy Swiss-Prot
Match: Q40061 (Mugineic-acid 3-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS2 PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 1.4e-31
Identity = 69/163 (42.33%), Postives = 100/163 (61.35%), Query Frame = 0


Query: 7   EIIDMISEGLGLEKGYLEGEMSSNRRIT-ANHYPICPNPSLTLGLNQHCDRNLITILFQD 66
           EI+ ++ EGLGL   +  G++S  R +   N+YP  PNPS TLGL  HCDR+L+T+L   
Sbjct: 161 EILRLLCEGLGLRPDFFVGDISGGRVVVDINYYPPSPNPSRTLGLPPHCDRDLMTVLLPG 220

Query: 67  A-NGLQVFKDGHWISFDSVDDAFLINFGYLFEVISNGKLKATEHRVVTNAKASRQSLGYF 126
           A  GL++   G WI    V ++ +INFG   EV++NG LKA EHR  TN    R S+  F
Sbjct: 221 AVPGLEIAYKGGWIKVQPVPNSLVINFGLQLEVVTNGYLKAVEHRAATNFAEPRLSVASF 280

Query: 127 IFPENDVIIEPAKCLINEANPARYRSLELEDFYRHFFAVSTNT 168
           I P +D ++ PA+  ++E NP RYR+L + +F R    V+ ++
Sbjct: 281 IVPADDCVVGPAEEFVSEDNPPRYRTLTVGEFKRKHNVVNLDS 323

[Close]

BLAST of Bhi09G000951 vs. ExPASy Swiss-Prot
Match: P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.2e-30
Identity = 66/158 (41.77%), Postives = 94/158 (59.49%), Query Frame = 0


Query: 1   MEKFSWEIIDMISEGLGLEKGYLEGEMSSNRRITANHYPICPNPSLTLGLNQHCDRNLIT 60
           + K +  ++D I EGLGL+ GY + E+S  + +  N+YP CP+PS TLG   H D NLIT
Sbjct: 165 VRKLTMRMLDYICEGLGLKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLIT 224

Query: 61  ILFQDANGLQ--VFKDGHWISFDSVDDAFLINFGYLFEVISNGKLKATEHRVVTNAKASR 120
           +L QD  GLQ  + KD  WI+   +  AF++N G   +VI+N K + + HRVVT+    R
Sbjct: 225 LLQQDLPGLQQLIVKDATWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDR 284

Query: 121 QSLGYFIFPENDVIIEPAKCLINEANPARYRSLELEDF 157
            S+   I P+    IEPAK L+N+ NP  Y+     +F
Sbjct: 285 VSIATLIGPDYSCTIEPAKELLNQDNPPLYKPYSYSEF 322

[Close]

BLAST of Bhi09G000951 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 2.7e-30
Identity = 69/165 (41.82%), Postives = 99/165 (60.00%), Query Frame = 0


Query: 8   IIDMISEGLGLEKGYLEGEMSSN-RRITANHYPICPNPSLTLGLNQHCDRNLITILFQD- 67
           +++ ISE LGL K  +   +  + + +  N+YP CP P LT GL  H D NLIT+L QD 
Sbjct: 173 LLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDE 232

Query: 68  ANGLQVFKDGHWISFDSVDDAFLINFGYLFEVISNGKLKATEHRVVTNAKASRQSLGYFI 127
            +GLQVFKDG WI+ + V + F++N G   +VISN K K+  HR V N+   R S+  F 
Sbjct: 233 VSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFY 292

Query: 128 FPENDVIIEPAKCLINEA--NPARYRSLELEDFYRHFFAVSTNTE 169
            P  D +I PA+ LINE   +PA YR+    +++  F+  + +TE
Sbjct: 293 CPSEDAVISPAQELINEEEDSPAIYRNFTYAEYFEKFWDTAFDTE 337

[Close]

BLAST of Bhi09G000951 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 5.0e-29
Identity = 64/174 (36.78%), Postives = 98/174 (56.32%), Query Frame = 0


Query: 1   MEKFSWEIIDMISEGLGLEKGYLEGEM-SSNRRITANHYPICPNPSLTLGLNQHCDRNLI 60
           + +  + + + ISE LGLE GY+   +    + +  N+YP CP P LT GL  H D N +
Sbjct: 164 VRELGFRLYEAISESLGLEGGYMRETLGEQEQHMAVNYYPQCPEPELTYGLPAHTDPNAL 223

Query: 61  TILFQD--ANGLQVFKDGHWISFDSVDDAFLINFGYLFEVISNGKLKATEHRVVTNAKAS 120
           TIL  D    GLQV  DG WI+ +    A +IN G   + +SNGK ++  HR V N+   
Sbjct: 224 TILLMDDQVAGLQVLNDGKWIAVNPQPGALVINIGDQLQALSNGKYRSVWHRAVVNSDRE 283

Query: 121 RQSLGYFIFPENDVIIEPAKCLINEANPARYRSLELEDFYRHFFAVSTNTEETM 172
           R S+  F+ P N V + PAK LI + +PA YR+   +++Y+ F++ + + E  +
Sbjct: 284 RMSVASFLCPCNSVELGPAKKLITDDSPAVYRNYTYDEYYKKFWSRNLDQEHCL 337

[Close]

BLAST of Bhi09G000951 vs. ExPASy TrEMBL
Match: A0A0A0K4U9 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G376360 PE=3 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 5.1e-77
Identity = 138/171 (80.70%), Postives = 153/171 (89.47%), Query Frame = 0


Query: 1   MEKFSWEIIDMISEGLGLEKGYLEGEMSSNRRITANHYPICPNPSLTLGLNQHCDRNLIT 60
           MEKFS EIID+ISEGLGL +GY EGEMSS RR+TANHYPICPNPSLT+GLNQHCDR+L+T
Sbjct: 158 MEKFSREIIDLISEGLGLGQGYFEGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMT 217

Query: 61  ILFQDANGLQVFKDGHWISFDSVDDAFLINFGYLFEVISNGKLKATEHRVVTNAKASRQS 120
           ILFQD +GLQVFKDGHWIS D +DDAF++NFGYL EVISNGKLKA EHRVVTNA+ASRQS
Sbjct: 218 ILFQDVSGLQVFKDGHWISIDPIDDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQS 277

Query: 121 LGYFIFPENDVIIEPAKCLINEANPARYRSLELEDFYRHFFAVSTNTEETM 172
            GY IFPE ++IIEPAKCLINEANP  YRS ++EDF RHFFAVS N EETM
Sbjct: 278 FGYVIFPEKEMIIEPAKCLINEANPPHYRSFKVEDFNRHFFAVSNNNEETM 328

[Close]

BLAST of Bhi09G000951 vs. ExPASy TrEMBL
Match: A0A5A7UWU7 (Hyoscyamine 6-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold54G00980 PE=3 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.5e-73
Identity = 134/171 (78.36%), Postives = 149/171 (87.13%), Query Frame = 0


Query: 1   MEKFSWEIIDMISEGLGLEKGYLEGEMSSNRRITANHYPICPNPSLTLGLNQHCDRNLIT 60
           MEKFS EIID+ISEGLGL +GY EGEMSS RRIT NHYPICPNPSLT+GLNQHCDR+LIT
Sbjct: 154 MEKFSQEIIDLISEGLGLGQGYFEGEMSSYRRITTNHYPICPNPSLTMGLNQHCDRDLIT 213

Query: 61  ILFQDANGLQVFKDGHWISFDSVDDAFLINFGYLFEVISNGKLKATEHRVVTNAKASRQS 120
           ILFQD +GLQVFKDGHWIS D ++ AF++NFGYL EVISNGKLKA EHRVVTNA+ASRQS
Sbjct: 214 ILFQDVDGLQVFKDGHWISIDPIEGAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQS 273

Query: 121 LGYFIFPENDVIIEPAKCLINEANPARYRSLELEDFYRHFFAVSTNTEETM 172
           L Y IFPE ++IIEPA CLINEANP  YRS ++EDF RHFFAVS N +E M
Sbjct: 274 LVYTIFPEKEMIIEPATCLINEANPPHYRSFKVEDFNRHFFAVSNNNDEMM 324

[Close]

BLAST of Bhi09G000951 vs. ExPASy TrEMBL
Match: A0A5D3DQY6 (Hyoscyamine 6-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00010 PE=4 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.5e-73
Identity = 134/171 (78.36%), Postives = 149/171 (87.13%), Query Frame = 0


Query: 1   MEKFSWEIIDMISEGLGLEKGYLEGEMSSNRRITANHYPICPNPSLTLGLNQHCDRNLIT 60
           MEKFS EIID+ISEGLGL +GY EGEMSS RRIT NHYPICPNPSLT+GLNQHCDR+LIT
Sbjct: 1   MEKFSQEIIDLISEGLGLGQGYFEGEMSSYRRITTNHYPICPNPSLTMGLNQHCDRDLIT 60

Query: 61  ILFQDANGLQVFKDGHWISFDSVDDAFLINFGYLFEVISNGKLKATEHRVVTNAKASRQS 120
           ILFQD +GLQVFKDGHWIS D ++ AF++NFGYL EVISNGKLKA EHRVVTNA+ASRQS
Sbjct: 61  ILFQDVDGLQVFKDGHWISIDPIEGAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQS 120

Query: 121 LGYFIFPENDVIIEPAKCLINEANPARYRSLELEDFYRHFFAVSTNTEETM 172
           L Y IFPE ++IIEPA CLINEANP  YRS ++EDF RHFFAVS N +E M
Sbjct: 121 LVYTIFPEKEMIIEPATCLINEANPPHYRSFKVEDFNRHFFAVSNNNDEMM 171

[Close]

BLAST of Bhi09G000951 vs. ExPASy TrEMBL
Match: A0A1S3BS12 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492870 PE=3 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 2.0e-73
Identity = 134/171 (78.36%), Postives = 149/171 (87.13%), Query Frame = 0


Query: 1   MEKFSWEIIDMISEGLGLEKGYLEGEMSSNRRITANHYPICPNPSLTLGLNQHCDRNLIT 60
           MEKFS EIID+ISEGLGL +GY EGEMSS RRIT NHYPICPNPSLT+GLNQHCDR+LIT
Sbjct: 154 MEKFSREIIDLISEGLGLGQGYFEGEMSSYRRITTNHYPICPNPSLTMGLNQHCDRDLIT 213

Query: 61  ILFQDANGLQVFKDGHWISFDSVDDAFLINFGYLFEVISNGKLKATEHRVVTNAKASRQS 120
           ILFQD +GLQVFKDGHWIS D ++ AF++NFGYL EVISNGKLKA EHRVVTNAKASRQS
Sbjct: 214 ILFQDVDGLQVFKDGHWISIDPIEGAFVVNFGYLLEVISNGKLKAVEHRVVTNAKASRQS 273

Query: 121 LGYFIFPENDVIIEPAKCLINEANPARYRSLELEDFYRHFFAVSTNTEETM 172
           L Y IFPE ++II+PA CLINEANP  YRS ++EDF RHFFAVS N +E M
Sbjct: 274 LVYTIFPEKEMIIKPATCLINEANPPHYRSFKVEDFNRHFFAVSNNNDEMM 324

[Close]

BLAST of Bhi09G000951 vs. ExPASy TrEMBL
Match: A0A0A0K5H0 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375850 PE=3 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 6.3e-59
Identity = 109/171 (63.74%), Postives = 130/171 (76.02%), Query Frame = 0


Query: 1   MEKFSWEIIDMISEGLGLEKGYLEGEMSSNRRITANHYPICPNPSLTLGLNQHCDRNLIT 60
           +EKF+ EI++++SEGLGLEKGY  GEMS N    A+HYP CPNPSLT+GL+QH D  LIT
Sbjct: 159 IEKFASEILEVLSEGLGLEKGYFRGEMSKNPEFLAHHYPPCPNPSLTVGLSQHADPTLIT 218

Query: 61  ILFQDANGLQVFKDGHWISFDSVDDAFLINFGYLFEVISNGKLKATEHRVVTNAKASRQS 120
           ILFQD NGLQ  KDG WI  D + DAFL+N GY+FEVISNGKLKA EHRVVTN+  SRQ+
Sbjct: 219 ILFQDVNGLQFLKDGQWIDVDPLPDAFLVNLGYVFEVISNGKLKAAEHRVVTNSTTSRQT 278

Query: 121 LGYFIFPENDVIIEPAKCLINEANPARYRSLELEDFYRHFFAVSTNTEETM 172
           L Y ++PENDV IEP+KC INEANP  YRSLE +DFY    +   + +  M
Sbjct: 279 LVYVVYPENDVTIEPSKCFINEANPPHYRSLEFKDFYNKLMSKPPDRKTAM 329

The following BLAST results are available for this feature:

BLAST of Bhi09G000951 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10490.1	1.9e-31	41.82	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G05010.1	2.6e-28	38.33	ethylene-forming enzyme	[more]
AT4G10500.1	3.3e-28	38.65	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G24530.1	2.8e-27	35.39	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19000.1	3.7e-27	37.89	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

BLAST of Bhi09G000951 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q40062	1.8e-34	44.03	2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=...	[more]
Q40061	1.4e-31	42.33	Mugineic-acid 3-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS2 PE=2 SV=1	[more]
P24397	1.2e-30	41.77	Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1	[more]
Q9ZSA7	2.7e-30	41.82	Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1	[more]
Q8W2X5	5.0e-29	36.78	Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1...	[more]

BLAST of Bhi09G000951 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K4U9	5.1e-77	80.70	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G...	[more]
A0A5A7UWU7	1.5e-73	78.36	Hyoscyamine 6-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A5D3DQY6	1.5e-73	78.36	Hyoscyamine 6-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3BS12	2.0e-73	78.36	hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492870 PE=3 SV=1	[more]
A0A0A0K5H0	6.3e-59	63.74	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..171 e-value: 1.6E-50 score: 174.2
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 35..127 e-value: 7.4E-22 score: 77.7
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 1..170
None	No IPR available	PANTHER	PTHR47991:SF31	1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE	coord: 1..170
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 1..162
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 28..127 score: 11.44638

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M000951	Bhi09M000951	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding