Bhi09G000855 (gene) Wax gourd (B227) v1

Overview
NameBhi09G000855
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionBasic 7S globulin 2
Locationchr9: 23908490 .. 23909981 (+)
RNA-Seq ExpressionBhi09G000855
SyntenyBhi09G000855
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTGTTTCTCTATAGAAAGAGTTCGTTTCAAACTCTAAATTGCATGCTTTGTATTGAAAGCAAAAATGGCCACTCCATTCTTCTTCTTCTTCTTCTTTCTTCTTTTTTTTCCTTTGTATTCTCTCCAAACGGCCTTCGTCGCTCCCATTTACAAAGACCATATCTCCCTTCTCTACTCCATCTCCGTCCACCTCAAAACGCCGCTCCGGCCGGCCAACCTCCACCTCGACCTCGGCGGCGGCTTCTCCTGGATCGACTGCTACAACCGTTACAACTCTTCCTCTTACCAATTTGTCCTTTGTAATTCCCCTCTCTCCCATTCTTTACAACAGAATACTTGCGGCTCCTGCGTCGAAGCTCCATCTCCGGTCTGCGCCAACGACACCATCTTTTCCTACGCCTATCCAGAGAAACCATTCCTCAGAGATCAATTTGTTGATTACAATCACGCTAAGCTCACCGATTCCGAGAATGTCATCACCGATGTTCTTGCTCTCTTCACCACTGACGGCTCCGATTCCTATCCACTCCGTCGTATTCCCGAAATTCCTTTCTCCTGCGTCAAGACCGATTTCCTCCGAGGACTTGCCAGGGGTGTCATTGGCCTGGCGGCGCTCGGTCGTTCCAACGTGTCGATTCCATCGGTGATTAGCGCGAAATTCAATAGCCCTAGGTTTTTCGCGATTTGTTTATCTGGAGCGAGATTAGGGACTGGCGTTGCTTTCTTCGGTTCTAAAGGTCCGTACAAATTTTTCCCCAATGTCGATCTTTCTAAATCCCTAATTTACACTCCCTTGCTCTTCAGTCCGGCGACCAACTCGATTTACACCAATTGGTTACCGTCTTACGAGTATTACATTGGACTCTCGGCCATTAGAATCAACGGCAAGGCAGTGCCTTTCAACACTTCTCTATTGTCGTTTGAGCCGGTTATTGGTGGCGGCGGAACGAAAATCAGCACCTCCACCAATTACGCGTTGCTACAGAGCTCAATTTACAGAGCATTCACGACGGTGTTTATGAAAGAATCTGCTGCACTGAACTTCACGTTGACAAATGCGGTAGAGCCGTTCGGAGTGTGCTATACGGCGTATAGCGTGGGAGTGACGGCGGAAGGACAGGCGAGGGCACCGGTGGTGGATCTGGTTATGGAGAAAGGGAAAGTGGTGTGGAAATTGGGGGGGAGGAATACGATGGTGAGGATTAAGAAGAATGGAGTTGATGTTTGGTGCTTGGGATTCATCAATGGTGGAGAATTTCCAAGAACGCCGATTGTGATCGGAGGTCTGCAAATGGAAGATCATTTGTTGCAGTTTGATCTTGAAAATTATAGATTTGGATTCAGTTCTTCGGCGCTAATGGAAGGGACTTCATGTTCAAAATTCAACTTCACTTCTATCAACAACATTTCCTTATAAGTGGCTGTGTGAGGTGCAGAATTGATATAAGATATCTGAAATTTTGTATTTGGGGCTACAGAGTTGATATGG

mRNA sequence

GTTTTGTTTCTCTATAGAAAGAGTTCGTTTCAAACTCTAAATTGCATGCTTTGTATTGAAAGCAAAAATGGCCACTCCATTCTTCTTCTTCTTCTTCTTTCTTCTTTTTTTTCCTTTGTATTCTCTCCAAACGGCCTTCGTCGCTCCCATTTACAAAGACCATATCTCCCTTCTCTACTCCATCTCCGTCCACCTCAAAACGCCGCTCCGGCCGGCCAACCTCCACCTCGACCTCGGCGGCGGCTTCTCCTGGATCGACTGCTACAACCGTTACAACTCTTCCTCTTACCAATTTGTCCTTTGTAATTCCCCTCTCTCCCATTCTTTACAACAGAATACTTGCGGCTCCTGCGTCGAAGCTCCATCTCCGGTCTGCGCCAACGACACCATCTTTTCCTACGCCTATCCAGAGAAACCATTCCTCAGAGATCAATTTGTTGATTACAATCACGCTAAGCTCACCGATTCCGAGAATGTCATCACCGATGTTCTTGCTCTCTTCACCACTGACGGCTCCGATTCCTATCCACTCCGTCGTATTCCCGAAATTCCTTTCTCCTGCGTCAAGACCGATTTCCTCCGAGGACTTGCCAGGGGTGTCATTGGCCTGGCGGCGCTCGGTCGTTCCAACGTGTCGATTCCATCGGTGATTAGCGCGAAATTCAATAGCCCTAGGTTTTTCGCGATTTGTTTATCTGGAGCGAGATTAGGGACTGGCGTTGCTTTCTTCGGTTCTAAAGGTCCGTACAAATTTTTCCCCAATGTCGATCTTTCTAAATCCCTAATTTACACTCCCTTGCTCTTCAGTCCGGCGACCAACTCGATTTACACCAATTGGTTACCGTCTTACGAGTATTACATTGGACTCTCGGCCATTAGAATCAACGGCAAGGCAGTGCCTTTCAACACTTCTCTATTGTCGTTTGAGCCGGTTATTGGTGGCGGCGGAACGAAAATCAGCACCTCCACCAATTACGCGTTGCTACAGAGCTCAATTTACAGAGCATTCACGACGGTGTTTATGAAAGAATCTGCTGCACTGAACTTCACGTTGACAAATGCGGTAGAGCCGTTCGGAGTGTGCTATACGGCGTATAGCGTGGGAGTGACGGCGGAAGGACAGGCGAGGGCACCGGTGGTGGATCTGGTTATGGAGAAAGGGAAAGTGGTGTGGAAATTGGGGGGGAGGAATACGATGGTGAGGATTAAGAAGAATGGAGTTGATGTTTGGTGCTTGGGATTCATCAATGGTGGAGAATTTCCAAGAACGCCGATTGTGATCGGAGGTCTGCAAATGGAAGATCATTTGTTGCAGTTTGATCTTGAAAATTATAGATTTGGATTCAGTTCTTCGGCGCTAATGGAAGGGACTTCATGTTCAAAATTCAACTTCACTTCTATCAACAACATTTCCTTATAAGTGGCTGTGTGAGGTGCAGAATTGATATAAGATATCTGAAATTTTGTATTTGGGGCTACAGAGTTGATATGG

Coding sequence (CDS)

ATGGCCACTCCATTCTTCTTCTTCTTCTTCTTTCTTCTTTTTTTTCCTTTGTATTCTCTCCAAACGGCCTTCGTCGCTCCCATTTACAAAGACCATATCTCCCTTCTCTACTCCATCTCCGTCCACCTCAAAACGCCGCTCCGGCCGGCCAACCTCCACCTCGACCTCGGCGGCGGCTTCTCCTGGATCGACTGCTACAACCGTTACAACTCTTCCTCTTACCAATTTGTCCTTTGTAATTCCCCTCTCTCCCATTCTTTACAACAGAATACTTGCGGCTCCTGCGTCGAAGCTCCATCTCCGGTCTGCGCCAACGACACCATCTTTTCCTACGCCTATCCAGAGAAACCATTCCTCAGAGATCAATTTGTTGATTACAATCACGCTAAGCTCACCGATTCCGAGAATGTCATCACCGATGTTCTTGCTCTCTTCACCACTGACGGCTCCGATTCCTATCCACTCCGTCGTATTCCCGAAATTCCTTTCTCCTGCGTCAAGACCGATTTCCTCCGAGGACTTGCCAGGGGTGTCATTGGCCTGGCGGCGCTCGGTCGTTCCAACGTGTCGATTCCATCGGTGATTAGCGCGAAATTCAATAGCCCTAGGTTTTTCGCGATTTGTTTATCTGGAGCGAGATTAGGGACTGGCGTTGCTTTCTTCGGTTCTAAAGGTCCGTACAAATTTTTCCCCAATGTCGATCTTTCTAAATCCCTAATTTACACTCCCTTGCTCTTCAGTCCGGCGACCAACTCGATTTACACCAATTGGTTACCGTCTTACGAGTATTACATTGGACTCTCGGCCATTAGAATCAACGGCAAGGCAGTGCCTTTCAACACTTCTCTATTGTCGTTTGAGCCGGTTATTGGTGGCGGCGGAACGAAAATCAGCACCTCCACCAATTACGCGTTGCTACAGAGCTCAATTTACAGAGCATTCACGACGGTGTTTATGAAAGAATCTGCTGCACTGAACTTCACGTTGACAAATGCGGTAGAGCCGTTCGGAGTGTGCTATACGGCGTATAGCGTGGGAGTGACGGCGGAAGGACAGGCGAGGGCACCGGTGGTGGATCTGGTTATGGAGAAAGGGAAAGTGGTGTGGAAATTGGGGGGGAGGAATACGATGGTGAGGATTAAGAAGAATGGAGTTGATGTTTGGTGCTTGGGATTCATCAATGGTGGAGAATTTCCAAGAACGCCGATTGTGATCGGAGGTCTGCAAATGGAAGATCATTTGTTGCAGTTTGATCTTGAAAATTATAGATTTGGATTCAGTTCTTCGGCGCTAATGGAAGGGACTTCATGTTCAAAATTCAACTTCACTTCTATCAACAACATTTCCTTATAA

Protein sequence

MATPFFFFFFFLLFFPLYSLQTAFVAPIYKDHISLLYSISVHLKTPLRPANLHLDLGGGFSWIDCYNRYNSSSYQFVLCNSPLSHSLQQNTCGSCVEAPSPVCANDTIFSYAYPEKPFLRDQFVDYNHAKLTDSENVITDVLALFTTDGSDSYPLRRIPEIPFSCVKTDFLRGLARGVIGLAALGRSNVSIPSVISAKFNSPRFFAICLSGARLGTGVAFFGSKGPYKFFPNVDLSKSLIYTPLLFSPATNSIYTNWLPSYEYYIGLSAIRINGKAVPFNTSLLSFEPVIGGGGTKISTSTNYALLQSSIYRAFTTVFMKESAALNFTLTNAVEPFGVCYTAYSVGVTAEGQARAPVVDLVMEKGKVVWKLGGRNTMVRIKKNGVDVWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFSSSALMEGTSCSKFNFTSINNISL
Homology
BLAST of Bhi09G000855 vs. TAIR 10
Match: AT1G03220.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 308.1 bits (788), Expect = 1.1e-83
Identity = 184/449 (40.98%), Postives = 265/449 (59.02%), Query Frame = 0

Query: 3   TPFFFFFFFLLFFPL-YSLQTAF-----VAPIYKDHISLLYSISVHLKTPLRPANLHLDL 62
           +P  F    L  F L  S QT F     + P+ KD  +L Y+  ++ +TPL PA++  DL
Sbjct: 4   SPIIFSVLLLFIFSLSSSAQTPFRPKALLLPVTKDQSTLQYTTVINQRTPLVPASVVFDL 63

Query: 63  GGGFSWIDCYNRYNSSSYQFVLCNSPLSHSLQQNTCGSCVEAPSPVCANDTIFSYAYPEK 122
           GG   W+DC   Y SS+YQ   CNS +       +CG+C   P P C+N+T         
Sbjct: 64  GGRELWVDCDKGYVSSTYQSPRCNSAVCSRAGSTSCGTCFSPPRPGCSNNTC-------- 123

Query: 123 PFLRDQFVDYNHAKLTDSENVITDVLALFTTDGSDSYPLRRIPEIPFSCVKTDFLRGLAR 182
                   D        S     DV+++ +T+GS+   + +IP + F C  T  L+GLA+
Sbjct: 124 ----GGIPDNTVTGTATSGEFALDVVSIQSTNGSNPGRVVKIPNLIFDCGATFLLKGLAK 183

Query: 183 GVIGLAALGRSNVSIPSVISAKFNSPRFFAICLSGARLGTGVAFFGSKGPYKFFPNVDLS 242
           G +G+A +GR N+ +PS  +A F+  R FA+CL+    G GVAFFG+ GPY F P + +S
Sbjct: 184 GTVGMAGMGRHNIGLPSQFAAAFSFHRKFAVCLTS---GKGVAFFGN-GPYVFLPGIQIS 243

Query: 243 KSLIYTPLLFSP-ATNSIYTNWLPSYEYYIGLSAIRINGKAVPFNTSLLSFEPVIGGGGT 302
            SL  TPLL +P +T S ++    S EY+IG++AI+I  K VP N +LL      G GGT
Sbjct: 244 -SLQTTPLLINPVSTASAFSQGEKSSEYFIGVTAIQIVEKTVPINPTLLKINASTGIGGT 303

Query: 303 KISTSTNYALLQSSIYRAFTTVFMKESAALNFTLTNAVEPFGVCYTAYSVGVTAEGQARA 362
           KIS+   Y +L+SSIY AFT+ F+K++AA +     +V+PFG C++  +VGVT  G A  
Sbjct: 304 KISSVNPYTVLESSIYNAFTSEFVKQAAARSIKRVASVKPFGACFSTKNVGVTRLGYA-V 363

Query: 363 PVVDLVMEKGKVVWKLGGRNTMVRIKKNGVDVWCLGFINGGEFPRTPIVIGGLQMEDHLL 422
           P ++LV+    VVW++ G N+MV +     DV CLGF++GG   RT +VIGG Q+ED+L+
Sbjct: 364 PEIELVLHSKDVVWRIFGANSMVSVSD---DVICLGFVDGGVNARTSVVIGGFQLEDNLI 423

Query: 423 QFDLENYRFGFSSSALMEGTSCSKFNFTS 445
           +FDL + +FGFSS+ L   T+C+ FNFTS
Sbjct: 424 EFDLASNKFGFSSTLLGRQTNCANFNFTS 431

BLAST of Bhi09G000855 vs. TAIR 10
Match: AT1G03230.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 300.4 bits (768), Expect = 2.4e-81
Identity = 173/445 (38.88%), Postives = 261/445 (58.65%), Query Frame = 0

Query: 10  FFLLFFPLYSLQT---------AFVAPIYKDHISLLYSISVHLKTPLRPANLHLDLGGGF 69
           F +L   ++SL +         A + P+ KD  +L Y+  ++ +TPL PA++  DLGG  
Sbjct: 9   FSVLLLSIFSLSSSAQPSFRPKALLLPVTKDPSTLQYTTVINQRTPLVPASVVFDLGGRE 68

Query: 70  SWIDCYNRYNSSSYQFVLCNSPLSHSLQQNTCGSCVEAPSPVCANDTIFSYAYPEKPFLR 129
            W+DC   Y S++Y+   CNS +        CG+C   P P C+N+T             
Sbjct: 69  FWVDCDQGYVSTTYRSPRCNSAVCSRAGSIACGTCFSPPRPGCSNNTC------------ 128

Query: 130 DQFVDYNHAKLTDSENVITDVLALFTTDGSDSYPLRRIPEIPFSCVKTDFLRGLARGVIG 189
             F D +      S     DV+++ +T+GS+     +IP + FSC  T  L+GLA+G +G
Sbjct: 129 GAFPDNSITGWATSGEFALDVVSIQSTNGSNPGRFVKIPNLIFSCGSTSLLKGLAKGAVG 188

Query: 190 LAALGRSNVSIPSVISAKFNSPRFFAICLSGARLGTGVAFFGSKGPYKFFPNVDLSKSLI 249
           +A +GR N+ +P   +A F+  R FA+CL+    G GVAFFG+ GPY F P + +S+ L 
Sbjct: 189 MAGMGRHNIGLPLQFAAAFSFNRKFAVCLTS---GRGVAFFGN-GPYVFLPGIQISR-LQ 248

Query: 250 YTPLLFSPATNSI-YTNWLPSYEYYIGLSAIRINGKAVPFNTSLLSFEPVIGGGGTKIST 309
            TPLL +P T    ++    S EY+IG++AI+I  K +P + +LL      G GGTKIS+
Sbjct: 249 KTPLLINPGTTVFEFSKGEKSPEYFIGVTAIKIVEKTLPIDPTLLKINASTGIGGTKISS 308

Query: 310 STNYALLQSSIYRAFTTVFMKESAALNFTLTNAVEPFGVCYTAYSVGVTAEGQARAPVVD 369
              Y +L+SSIY+AFT+ F++++AA +     +V+PFG C++  +VGVT  G A  P + 
Sbjct: 309 VNPYTVLESSIYKAFTSEFIRQAAARSIKRVASVKPFGACFSTKNVGVTRLGYA-VPEIQ 368

Query: 370 LVMEKGKVVWKLGGRNTMVRIKKNGVDVWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 429
           LV+    VVW++ G N+MV +     DV CLGF++GG  P   +VIGG Q+ED+L++FDL
Sbjct: 369 LVLHSKDVVWRIFGANSMVSVSD---DVICLGFVDGGVNPGASVVIGGFQLEDNLIEFDL 428

Query: 430 ENYRFGFSSSALMEGTSCSKFNFTS 445
            + +FGFSS+ L   T+C+ FNFTS
Sbjct: 429 ASNKFGFSSTLLGRQTNCANFNFTS 432

BLAST of Bhi09G000855 vs. TAIR 10
Match: AT5G19110.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 164.9 bits (416), Expect = 1.5e-40
Identity = 125/436 (28.67%), Postives = 201/436 (46.10%), Query Frame = 0

Query: 11  FLLFFPLYSLQ--TAFVAPIYKDHISLLYSISVHLKTPLR-PANLHLDLGGGFSWIDCYN 70
           FL  F   +L+  + ++ PI K   + L+  + ++ +  + P NL LDLG   +W+DC  
Sbjct: 11  FLSIFAAIALKSNSQYLLPITKHEPTNLFYTTFNVGSAAKSPVNLLLDLGTNLTWLDCRK 70

Query: 71  RYNSSSYQFVLCNSPLSHSLQQNTCG--SCV-EAPSPVCANDTIFSYAYPEKPFLRDQFV 130
             + SS + V C S    S+  N C   SC+ + P+P+  N  +                
Sbjct: 71  LKSLSSLRLVTCQSSTCKSIPGNGCAGKSCLYKQPNPLGQNPVV---------------- 130

Query: 131 DYNHAKLTDSENVITDVLALFTTDGSDSYPLRRIPEIPFSCVKTDFLRGLARGVIGLAAL 190
                    +  V+ D  +L+TTDG        +    FSC     L+GL   V G+ AL
Sbjct: 131 ---------TGRVVQDRASLYTTDGGKFLSQVSVRHFTFSCAGEKALQGLPPPVDGVLAL 190

Query: 191 GRSNVSIPSVISAKFNSPRFFAICLSGARLGTGVAFFGSKGPYKFFPNVDLSKSLIYTPL 250
              + S    +++ FN    F++CL      +G   F   G + F P  + S + I  P 
Sbjct: 191 SPGSSSFTKQVTSAFNVIPKFSLCLP----SSGTGHFYIAGIHYFIPPFNSSDNPI--PR 250

Query: 251 LFSPATNSIYTNWLPSYEYYIGLSAIRINGKAVPFNTSLLSFEPVIGGGGTKISTSTNYA 310
             +P   +       S +Y I + +I + G A+  N  LL+       GG K+ST  +Y 
Sbjct: 251 TLTPIKGT------DSGDYLITVKSIYVGGTALKLNPDLLT-------GGAKLSTVVHYT 310

Query: 311 LLQSSIYRAFTTVFMKESAALNFTLTNAVEPFGVCYTAYSVGVTAEGQARAPVVDLVM-- 370
           +LQ+ IY A    F  ++ A+      +V PF  C+ + + G         PV+++ +  
Sbjct: 311 VLQTDIYNALAQSFTLKAKAMGIAKVPSVAPFKHCFDSRTAGKNLTAGPNVPVIEIGLPG 370

Query: 371 EKGKVVWKLGGRNTMVRIKKNGVDVWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENY 430
             G+V W   G NT+V++K+    V CL FI+GG+ P+  +VIG  Q++DH+L+FD    
Sbjct: 371 RIGEVKWGFYGANTVVKVKET---VMCLAFIDGGKTPKDLMVIGTHQLQDHMLEFDFSGT 399

Query: 431 RFGFSSSALMEGTSCS 439
              FS S L+  TSCS
Sbjct: 431 VLAFSESLLLHNTSCS 399

BLAST of Bhi09G000855 vs. TAIR 10
Match: AT5G19100.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 148.3 bits (373), Expect = 1.5e-35
Identity = 143/450 (31.78%), Postives = 210/450 (46.67%), Query Frame = 0

Query: 1   MATPFFFFFFFLLFFPL----YSLQ--TAFVAPIYKDHISLLYSISVHLKTPLRPANLHL 60
           MA    F    L+F  L    +SL+   +F+ PIYKD    +Y+I              L
Sbjct: 1   MAPRVIFLLLSLVFLYLANTSHSLRKFQSFLHPIYKDTAKNIYTIP-------------L 60

Query: 61  DLGGGFSWIDCYNRYNSSSYQFVLCNSPLSHSLQQNTCGSCVEAPSPVCANDTIFSYAYP 120
            +G            ++SS +FVL  +  +  LQ     +      P+    T   YA P
Sbjct: 61  SIG------------STSSEKFVLDLNGAAPLLQNCPTAAKSTTYHPIRCGSTRCKYANP 120

Query: 121 EKPFLRDQFVDYNHAKL-TDSENVITD-VLALFTTDGSDSYPLRRIPEIPFSCVKTDFLR 180
             P   +         L +D+  +  D V  L+T +G  +        +  +C  TD   
Sbjct: 121 NFPCPNNVIAKKRTVCLSSDNSRLFRDTVPLLYTFNGVYTRDSEMSSSLTLTC--TDGAP 180

Query: 181 GLARGVIGLAALGRSNVSIPSVISAKFNSPRFFAICLSG---ARLGTGVAFFGSKGPYKF 240
            L +  IGLA    +++SIPS + + +  P   A+CL     ++   G  + G KG Y +
Sbjct: 181 ALKQRTIGLA---NTHLSIPSQLISMYQLPHKIALCLPSTERSQSHNGDLWIG-KGEYYY 240

Query: 241 FP-NVDLSKSLIYTPLLFSPATNSIYTNWLPSYEYYIGLSAIRINGKAVPFNTSLLSFEP 300
            P + D+SK    TPL+ +            S EY I + +I+I  K VP          
Sbjct: 241 LPYDKDVSKIFASTPLIGNG----------KSGEYLIDVKSIQIGAKTVP---------- 300

Query: 301 VIGGGGTKISTSTNYALLQSSIYRAFTTVFMKESAALNFTLTNAVEPFGVCYTAYSVGVT 360
            I  G TKIST   Y + Q+S+Y+A  T F +    +      AV+PFG C+  YS    
Sbjct: 301 -IPYGATKISTLAPYTVFQTSLYKALLTAFTEN---IKIAKAPAVKPFGACF--YS---- 360

Query: 361 AEGQARAPVVDLVMEKGKVVWKLGGRNTMVRIKKNGVDVWCLGFINGGEFPRTPIVIGGL 420
             G    PV+DLV+  G   W++ G N++V++ KN   V CLGF++GG  P+ PIVIGG 
Sbjct: 361 -NGGRGVPVIDLVL-SGGAKWRIYGSNSLVKVNKN---VVCLGFVDGGVKPKYPIVIGGF 384

Query: 421 QMEDHLLQFDLENYRFGFSSSALMEGTSCS 439
           QMED+L++FDLE  +F FSSS L+  TSCS
Sbjct: 421 QMEDNLVEFDLEASKFSFSSSLLLHNTSCS 384

BLAST of Bhi09G000855 vs. TAIR 10
Match: AT5G19120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 141.7 bits (356), Expect = 1.4e-33
Identity = 130/437 (29.75%), Postives = 193/437 (44.16%), Query Frame = 0

Query: 5   FFFFFFFLLFFPLYSLQ---TAFVAPIYKDHISLLYSISVHLKTPLRPANLHLDLGGGFS 64
           FFF F   L      +       V P+ KD  +  Y   + L     P  L +DL G   
Sbjct: 10  FFFSFLSALIISKSQISDSVNGVVFPVVKDLPTGQYLAQIRLGDSPDPVKLVVDLAGSIL 69

Query: 65  WIDCYNRYNSSSYQFVLCNSPLSHSLQQNTCGSCVEAPSPVCANDTIFSYAYPEKPFLRD 124
           W DC +R+ SS           S +L   +   C++A      N+ + S +   K    D
Sbjct: 70  WFDCSSRHVSS-----------SRNLISGSSSGCLKAK---VGNERVSSSSSSRKDQNAD 129

Query: 125 --QFVDYNHAKLTDSENVITDVLALFTTDGSDSYPLRRIPEIPFSCVKTDFLRGLARGVI 184
               V  +   +T    + +DV+++    GS + P     ++ F+C     LRGLA G  
Sbjct: 130 CELLVKNDAFGITARGELFSDVMSV----GSVTSP--GTVDLLFACTPPWLLRGLASGAQ 189

Query: 185 GLAALGRSNVSIPSVISAKFNSPRFFAICLSGARLGTGVAFFGSKGPYKFFPNVDLSKSL 244
           G+  LGR+ +S+PS ++A+ N  R   + LS      GV    S    +    V  S+SL
Sbjct: 190 GVMGLGRAQISLPSQLAAETNERRRLTVYLSPL---NGVV---STSSVEEVFGVAASRSL 249

Query: 245 IYTPLLFSPATNSIYTNWLPSYEYYIGLSAIRINGKAVPFNTSLLSFEPVIGGGGTKIST 304
           +YTPLL   + N           Y I + +IR+NG+ +           V G    ++ST
Sbjct: 250 VYTPLLTGSSGN-----------YVINVKSIRVNGEKL----------SVEGPLAVELST 309

Query: 305 STNYALLQSSIYRAFTTVFMKESAALNFTLTNAVEPFGVCYTAYSVGVTAEGQARAPVVD 364
              Y +L+SSIY+ F   + K  AA   T    V PFG+C+T+             P VD
Sbjct: 310 VVPYTILESSIYKVFAEAYAK--AAGEATSVPPVAPFGLCFTS---------DVDFPAVD 369

Query: 365 LVMEKGKVVWKLGGRNTMVRIKKNGVDVWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 424
           L ++   V W++ G+N MV +   G  V C G ++GG     PIV+GGLQ+E  +L FDL
Sbjct: 370 LALQSEMVRWRIHGKNLMVDV---GGGVRCSGIVDGGSSRVNPIVMGGLQLEGFILDFDL 385

Query: 425 ENYRFGFSSSALMEGTS 437
            N   GF      + TS
Sbjct: 430 GNSMMGFGQRTRSDSTS 385

BLAST of Bhi09G000855 vs. ExPASy Swiss-Prot
Match: I1JNS6 (Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2)

HSP 1 Score: 331.6 bits (849), Expect = 1.3e-89
Identity = 193/443 (43.57%), Postives = 259/443 (58.47%), Query Frame = 0

Query: 7   FFFFFLLFFPLYSLQTAFVAPIYKDHISLLYSISVHLKTPLRPANLHLDLGGGFSWIDCY 66
           F    L  F   + Q   +API KD  + LY++SV LKTPL+P  LHL LG   SW+ C 
Sbjct: 11  FNLAILFLFLTPTFQIPLIAPISKDDTTQLYTLSVFLKTPLQPTKLHLHLGSSLSWVLCD 70

Query: 67  NRYNSSSYQFVLCNSPLSHSLQQNTCGSCVEAPSPVCANDTIFSYAYPEKPFLRDQFVDY 126
           + Y SSS   + CN+PL           C   PS  C+N++     +PE P  R+  +D 
Sbjct: 71  STYTSSSSHHIPCNTPL-----------CNSFPSNACSNNSSLCALFPENPVTRNTLLD- 130

Query: 127 NHAKLTDSENVITDVLALFTTDGSDSYPLRRIPEIPFSCVKTDFLRGLARGVIGLAALGR 186
                      + D LAL T D S S  L  I +  FSC     L+GLA   +GLA+LGR
Sbjct: 131 ---------TALIDSLALPTYDASSSLVL--ISDFIFSCATAHLLQGLAANALGLASLGR 190

Query: 187 SNVSIPSVISAKFNSPRFFAICLSGARLGTGVAFFGS-KGPYKFFPNVDLSKSLIYTPLL 246
           SN S+P+ IS    SPR F +CL  +   TG A F S    + F   +DL+    YT L+
Sbjct: 191 SNYSLPAQISTSLTSPRSFTLCLPASSANTGAAIFASTASSFLFSSKIDLT----YTQLI 250

Query: 247 FSPATNSIYT-NWLPSYEYYIGLSAIRINGKAVPFNTSLLSFEPVIGGGGTKISTSTNYA 306
            +P  +++ T N  PS EY+I L++I+INGK +  N+S+L+ +   G GGTKIST+  Y 
Sbjct: 251 VNPVADTVVTDNPQPSDEYFINLTSIKINGKPLYINSSILTVDQT-GFGGTKISTAEPYT 310

Query: 307 LLQSSIYRAFTTVFMKESAALNFTLTNAVEPFGVCYTAYSVGVTAEGQARAPVVDLVMEK 366
           +L++SIYR F   F+ ES+A N T+T AVEPFGVCY A  +  T  G A  P VDLVM  
Sbjct: 311 VLETSIYRLFVQRFVNESSAFNLTVTEAVEPFGVCYPAGDLTETRVGPA-VPTVDLVMHS 370

Query: 367 GKVVWKLGGRNTMVRIKKNGVDVWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRF 426
             V W++ G N+MVR+ K GVDVWCLGF++GG   RTPIVIGG Q+ED+L+QFDL++ RF
Sbjct: 371 EDVFWRIFGGNSMVRVAKGGVDVWCLGFVDGGTRGRTPIVIGGHQLEDNLMQFDLDSNRF 424

Query: 427 GFSSSALMEGTSCSKFNFTSINN 448
           GF+S+ L++   CS     +  N
Sbjct: 431 GFTSTLLLQDAKCSNLKVNNFAN 424

BLAST of Bhi09G000855 vs. ExPASy Swiss-Prot
Match: P0DO21 (Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 2.1e-79
Identity = 176/426 (41.31%), Postives = 248/426 (58.22%), Query Frame = 0

Query: 25  VAPIYKDHISLLYSISVHLKTPLRPANLHLDLGGGFSWIDCYNRYNSSSYQFVLCNS-PL 84
           + PI KD ++L Y   +  +TPL P +L LDLGG F W+DC   Y SS+Y+   C S   
Sbjct: 35  ILPITKDALTLQYLTQIQQRTPLVPVSLTLDLGGQFLWVDCDQGYVSSTYRPARCRSAQC 94

Query: 85  SHSLQQNTCGSCVEAPSPVCANDTIFSYAYPEKPFLRDQFVDYNHAKLTDSENVITDVLA 144
           S +   + CG C   P P C N+T                 D    +   S  + +D + 
Sbjct: 95  SLAGAGSGCGQCFSPPKPGCNNNTC------------GLLPDNTITRTATSGELASDTVQ 154

Query: 145 LFTTDGSDSYPLRRI--PEIPFSCVKTDFLRGLARGVIGLAALGRSNVSIPSVISAKFNS 204
           + +++G +  P R +   +  F C  T  L GLA GV G+A LGR+ +S+PS  SA+F+ 
Sbjct: 155 VQSSNGKN--PGRHVSDKDFLFVCGSTFLLEGLASGVKGMAGLGRTRISLPSQFSAEFSF 214

Query: 205 PRFFAICLSGARLGTGVAFFGSKGPYKFFPNVDLSKS-LIYTPLLFSP-ATNSIYTNWLP 264
           PR FA+CLS +    GV  FG  GPY F PN + + +   YTPL  +P +T S +++  P
Sbjct: 215 PRKFAVCLSSSTNSKGVVLFGD-GPYTFLPNREFANNDFSYTPLFINPVSTASAFSSREP 274

Query: 265 SYEYYIGLSAIRINGKAVPFNTSLLSFEPVIGGGGTKISTSTNYALLQSSIYRAFTTVFM 324
           S EY+IG+ +I+IN K VP NT+LLS +   G GGTKIST   Y +L++SIY A T  F+
Sbjct: 275 SSEYFIGVKSIKINEKVVPINTTLLSIDNQ-GVGGTKISTVNPYTILETSIYNAVTNFFV 334

Query: 325 KESAALNFTLTNAVEPFGVCYTAYSVGVTAEGQARAPVVDLVMEKGKVVWKLGGRNTMVR 384
           KE   +N T   +V PF  C+ + ++  T  G A  P +DLV++   V W++ G N+MV+
Sbjct: 335 KE--LVNITRVASVAPFRACFDSRNIASTRVGPA-VPSIDLVLQNENVFWRIFGANSMVQ 394

Query: 385 IKKNGVDVWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFSSSALMEGTSCSK 444
           + +N   V CLGF++GG  PRT IV+GG  +ED+LLQFDL   R GF+SS L   T+C+ 
Sbjct: 395 VSEN---VLCLGFVDGGVSPRTSIVVGGYTIEDNLLQFDLARSRLGFTSSILFRQTTCAN 438

Query: 445 FNFTSI 446
           FNFTSI
Sbjct: 455 FNFTSI 438

BLAST of Bhi09G000855 vs. ExPASy Swiss-Prot
Match: P82952 (Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2)

HSP 1 Score: 219.2 bits (557), Expect = 9.7e-56
Identity = 144/427 (33.72%), Postives = 217/427 (50.82%), Query Frame = 0

Query: 25  VAPIYKDHISLLYSISVHLKTPLRPANLHLDLGGGFSWIDCYNRYNSSSYQFVLCNSPLS 84
           V  + KD  + L+ + +H +TPL      +DL G F  ++C N+Y SS+Y+  +C+S   
Sbjct: 39  VLKVQKDRATNLHVVQIHKRTPLVQFPFVIDLTGRFLSVNCENQYTSSTYKAPVCHSSQC 98

Query: 85  HSLQQNTCGSCVEAPS-PVCANDTIFSYAYPEKPFLRDQFVDYNHAKLTDSENVITDVLA 144
                +TC +C  + + P C  +            L    V    A+      +  DVL 
Sbjct: 99  ARANSHTCRTCSSSKTRPGCHTNAC--------GLLTTNPVTQQSAQ----GELAEDVLK 158

Query: 145 LFTTDGSDSYPLRRIPEIPFSCVKTDFL-RGLARGVIGLAALGRSNVSIPSVISAKFNSP 204
           + +T GS   P+   P   F+C  ++ L +GL + V G+A LG S +S+P  +++ F  P
Sbjct: 159 IPSTQGSSPGPMVTYPHFLFACAPSNILQKGLPKNVQGVAGLGHSPISLPYQLASHFGFP 218

Query: 205 RFFAICLSGARLGTGVAFFGSKGPYKFFPNVDLSKSLIYTPLLFSPATNSIYTNWLPSYE 264
             FA+CL+ +    G  FFG +GPY   P +D+S+ L Y P                  E
Sbjct: 219 PKFAVCLTSSPGKNGAVFFG-EGPYFMKPGIDVSRQLTYAPFTIG-----------QQGE 278

Query: 265 YYIGLSAIRINGKAVPFNTSLLSFEPVIGGGGTKISTSTNYALLQSSIYRAFTTVFMKES 324
           YYI + + +I       N ++L   P  G GG  IST+T Y  LQ+ I+RA   +FM + 
Sbjct: 279 YYINVQSFKI-------NNAMLPSIPKGGFGGAMISTTTPYTTLQTPIFRALNQLFMNQL 338

Query: 325 AALNFTLTNAVEPFGVCYTAYSVGVTAEGQARAPVVDLVME-KGKVVWKLGGRNTMVRIK 384
             +       V PFG C+ A  +  +  G    P +DLV++ K  ++W++ G N M++ +
Sbjct: 339 RGVPH--VKPVAPFGACFDANRIPTSKMGPT-VPSIDLVLDNKKNIMWRIFGANAMIQPR 398

Query: 385 KNGVDVWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFSSSALMEGTSCSKFN 444
                V CL F++GG  P+ PIVIG  Q+ED+LLQFDL N R GFSSS L   T+C+ FN
Sbjct: 399 PG---VMCLAFVDGGMRPKAPIVIGTQQLEDNLLQFDLMNSRLGFSSSLLFRRTNCANFN 428

Query: 445 F-TSINN 448
           F TS  N
Sbjct: 459 FGTSSTN 428

BLAST of Bhi09G000855 vs. ExPASy Swiss-Prot
Match: P13917 (Basic 7S globulin OS=Glycine max OX=3847 GN=BG PE=1 SV=2)

HSP 1 Score: 185.7 bits (470), Expect = 1.2e-45
Identity = 137/429 (31.93%), Postives = 211/429 (49.18%), Query Frame = 0

Query: 25  VAPIYKDHISLLYSISVHLKTPLRPANLHLDLGGGFSWIDCYNRYNSSSYQFVLCNSPLS 84
           V P+  D  + L+  ++  +TPL    + +DL G   W++C  +Y+S +YQ   C+S   
Sbjct: 35  VLPVQNDGSTGLHWANLQKRTPLMQVPVLVDLNGNHLWVNCEQQYSSKTYQAPFCHSTQC 94

Query: 85  HSLQQNTCGSCVEAPSPVCANDTIFSYAYPEKPFLRDQFVDYNHAKLTDSENVITDVLAL 144
                + C SC  A  P C  +T    +    P  +           T    +  DVLA+
Sbjct: 95  SRANTHQCLSCPAASRPGCHKNTCGLMS--TNPITQQ----------TGLGELGEDVLAI 154

Query: 145 FTTDGSDSY--PLRRIPEIPFSCVKTDFL-RGLARGVIGLAALGRSNVSIPSVISAKFNS 204
             T GS     PL  +P+  FSC  +  + +GL R   G+A LG + +S+P+ +++ F  
Sbjct: 155 HATQGSTQQLGPLVTVPQFLFSCAPSFLVQKGLPRNTQGVAGLGHAPISLPNQLASHFGL 214

Query: 205 PRFFAICLSGARLGTGVAFFG-SKGPYKFFPNVDLSKSLIYTPLLFSPATNSIYTNWLPS 264
            R F  CLS      G   FG +    + F N D+   L +TPL  +             
Sbjct: 215 QRQFTTCLSRYPTSKGAIIFGDAPNNMRQFQNQDIFHDLAFTPLTIT-----------LQ 274

Query: 265 YEYYIGLSAIRINGKAVPFNTSLLSFEPVIG--GGGTKISTSTNYALLQSSIYRAFTTVF 324
            EY + +++IRIN  +V F  + +S   ++G   GGT ISTST + +LQ S+Y+AFT VF
Sbjct: 275 GEYNVRVNSIRINQHSV-FPLNKIS-STIVGSTSGGTMISTSTPHMVLQQSVYQAFTQVF 334

Query: 325 MKESAALNFTLTNAVEPFGVCYTAYSVGVTAEGQARAPVVDLVMEK-GKVVWKLGGRNTM 384
            ++          +V PFG+C+ +  +          P VDLVM+K    VW++ G + M
Sbjct: 335 AQQLP--KQAQVKSVAPFGLCFNSNKINA-------YPSVDLVMDKPNGPVWRISGEDLM 394

Query: 385 VRIKKNGVDVWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFSSSAL-MEGTS 444
           V+ +     V CLG +NGG  PR  I +G  Q+E++L+ FDL   R GFS+S+L   G  
Sbjct: 395 VQAQPG---VTCLGVMNGGMQPRAEITLGARQLEENLVVFDLARSRVGFSTSSLHSHGVK 426

BLAST of Bhi09G000855 vs. ExPASy Swiss-Prot
Match: Q42369 (Gamma conglutin 1 OS=Lupinus angustifolius OX=3871 GN=LOC109345795 PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.2e-45
Identity = 137/447 (30.65%), Postives = 212/447 (47.43%), Query Frame = 0

Query: 16  PLYSLQTAFVAPIYKDHISLLYSISVHLKTPLRPANLHLDLGGGFSWIDCYNRYNSSSYQ 75
           P  S     V P+ +D  + L+  ++H +TPL    L LDL G   W+ C   Y+SS+YQ
Sbjct: 39  PTSSKPNLLVLPVQEDASTGLHWANIHKRTPLMQVPLLLDLNGKHLWVTCSQHYSSSTYQ 98

Query: 76  FVLCNSPLSHSLQQNTCGSCVEAPS--PVCANDTIFSYAYPEKPFLRDQFVDYNHAKLTD 135
              C+S        + C +C ++ +  P C N+T    +    P  ++  +         
Sbjct: 99  APFCHSTQCSRANTHQCFTCTDSTTTRPGCHNNTCGLLS--SNPVTQESGLG-------- 158

Query: 136 SENVITDVLALFTTDGSDSYPLRRIPEIPFSCVKTDFL--RGLARGVIGLAALGRSNVSI 195
              +  DVLA+ +T GS   P+ ++P+  FSC  + FL  +GL   V G   LG++ +S+
Sbjct: 159 --ELAQDVLAIHSTHGSKLGPMVKVPQFLFSCAPS-FLAQKGLPNNVQGALGLGQAPISL 218

Query: 196 PSVISAKFNSPRFFAICLSGARLGTGVAFFGS----KGPYKFFPNVDLSKSLIYTPLLFS 255
            + + + F   R F++CLS      G   FG             ++D+   L+YTPL  S
Sbjct: 219 QNQLFSHFGLKRQFSVCLSRYSTSNGAILFGDINDPNNNNYIHNSLDVLHDLVYTPLTIS 278

Query: 256 PATNSIYTNWLPSYEYYIGLSAIRINGKAV-----PF-NTSLLSFEPVIGGGGTKISTST 315
                         EY+I ++AIR+N   V     PF + S  S+      GG  I+T+ 
Sbjct: 279 -----------KQGEYFIQVNAIRVNKHLVIPTKNPFISPSSTSYHGSGEIGGALITTTH 338

Query: 316 NYALLQSSIYRAFTTVFMKESAALNFTLTNAVEPFGVCYTAYSVGVTAEGQARAPVVDLV 375
            Y +L  SI+  FT VF             AV PFG+CY +  +         AP VDL+
Sbjct: 339 PYTVLSHSIFEVFTQVFANNMP--KQAQVKAVGPFGLCYDSRKI------SGGAPSVDLI 398

Query: 376 MEKGKVVWKLGGRNTMVRIKKNGVDVWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLEN 435
           ++K   VW++   N MV+ +     V CLGF++GG   R  I +G   +E++L+ FDLE 
Sbjct: 399 LDKNDAVWRISSENFMVQAQDG---VSCLGFVDGGVHARAGIALGAHHLEENLVVFDLER 448

Query: 436 YRFGFSSSALME-GTSCSKFNFTSINN 448
            R GF+S++L   G +CS  N   +NN
Sbjct: 459 SRVGFNSNSLKSYGKTCS--NLFDLNN 448

BLAST of Bhi09G000855 vs. ExPASy TrEMBL
Match: A0A0A0K506 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G390050 PE=4 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 2.5e-211
Identity = 367/444 (82.66%), Postives = 397/444 (89.41%), Query Frame = 0

Query: 4   PFFFFFFFLLFFPLYSLQTAFVAPIYKDHISLLYSISVHLKTPLRPANLHLDLGGGFSWI 63
           P FFFFFFL+ FPLYSLQTA +AP+YK H SLLYSIS+HLKTPLRPA+L+LDLGG FSWI
Sbjct: 5   PLFFFFFFLISFPLYSLQTALIAPLYKHHTSLLYSISLHLKTPLRPASLYLDLGGAFSWI 64

Query: 64  DCYNRYNSSSYQFVLCNSPLSHSLQQNTCGSCVEAPSPVCANDTIFSYAYPEKPFLRDQF 123
            CY  YNSSSY+FVLCN+PLS+S  Q  CGSCV+APSP+CANDTIFSYAYPE P LRD F
Sbjct: 65  HCYQNYNSSSYKFVLCNTPLSNSFNQAICGSCVQAPSPICANDTIFSYAYPENPSLRDHF 124

Query: 124 VDYNHAKLTDSENVITDVLALFTTDGSDSYPLRRIPEIPFSCVKTDFLRGLARGVIGLAA 183
           VDY+H KLTDSENVITDVLAL TT GS S PLRRIPE PF+CVKT+FLR +A+ VIGLAA
Sbjct: 125 VDYDHPKLTDSENVITDVLALSTTGGSTSAPLRRIPEFPFACVKTNFLREVAKNVIGLAA 184

Query: 184 LGRSNVSIPSVISAKFNSPRFFAICLSGARLGTGVAFFGSKGPYKFFPNVDLSKSLIYTP 243
           LGRSN+SIPSVISAKF+SP++FAICLSGAR G GVAFFGSKGPY+F PNVDLSKSL YTP
Sbjct: 185 LGRSNLSIPSVISAKFSSPKYFAICLSGARSGPGVAFFGSKGPYRFSPNVDLSKSLTYTP 244

Query: 244 LLFSPATNSIYTNWLPSYEYYIGLSAIRINGKAVPFNTSLLSFEPVIGGGGTKISTSTNY 303
           LLF+P + SIYT WLPSYEYY+GLSAIRINGK VPFNTSLLSFEP+ G GG KISTSTNY
Sbjct: 245 LLFNPVSASIYTYWLPSYEYYVGLSAIRINGKVVPFNTSLLSFEPIHGRGGAKISTSTNY 304

Query: 304 ALLQSSIYRAFTTVFMKESAALNFTLTNAVEPFGVCYTAYSVGVTAEGQARAPVVDLVME 363
           ALL+SSIYRAF TVFMKE+  LNF L NAVEPFGVCY A SVGVTAEGQA+APVVDLVME
Sbjct: 305 ALLRSSIYRAFATVFMKEAVVLNFKLINAVEPFGVCYEAKSVGVTAEGQAKAPVVDLVME 364

Query: 364 KGKVVWKLGGRNTMVRIKKNGVDVWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYR 423
           K KVVWKLGGRNTMVRIKK GVD WCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLEN+R
Sbjct: 365 KEKVVWKLGGRNTMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENFR 424

Query: 424 FGFSSSALMEGTSCSKFNFTSINN 448
           FGFSSSAL EGTSCSKF+FTS NN
Sbjct: 425 FGFSSSALKEGTSCSKFDFTSANN 448

BLAST of Bhi09G000855 vs. ExPASy TrEMBL
Match: A0A5D3D4L4 (Basic 7S globulin 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2032G00050 PE=4 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 7.1e-211
Identity = 368/448 (82.14%), Postives = 400/448 (89.29%), Query Frame = 0

Query: 1   MATP-FFFFFFFLLFFPLYSLQTAFVAPIYKDHISLLYSISVHLKTPLRPANLHLDLGGG 60
           M+TP  FFFFFFL+ FP YSLQTA VAP++KDHISLLYSIS+HLKTPLRPA L+LDLGG 
Sbjct: 1   MSTPLLFFFFFFLISFPSYSLQTASVAPLFKDHISLLYSISLHLKTPLRPATLYLDLGGP 60

Query: 61  FSWIDCYNRYNSSSYQFVLCNSPLSHSLQQNTCGSCVEAPSPVCANDTIFSYAYPEKPFL 120
           FSWIDCY  YNSSSY+ +LCN+PLS+S  Q  CGSCV+APSP CANDTIF+YAYP+ P L
Sbjct: 61  FSWIDCYQNYNSSSYKLLLCNTPLSNSFNQGICGSCVQAPSPTCANDTIFTYAYPQNPSL 120

Query: 121 RDQFVDYNHAKLTDSENVITDVLALFTTDGSDSYPLRRIPEIPFSCVKTDFLRGLARGVI 180
           RDQFVDY+  +LTDSENVITDVLAL TTDGS S PLRRI EIPF+CVKT+FLRGLA+ VI
Sbjct: 121 RDQFVDYDRPELTDSENVITDVLALSTTDGSKSGPLRRISEIPFACVKTNFLRGLAKNVI 180

Query: 181 GLAALGRSNVSIPSVISAKFNSPRFFAICLSGARLGTGVAFFGSKGPYKFFPNVDLSKSL 240
           GLAALGRSN+SIPSVISAKFNSP+FFAICLSGAR G GVAFFGSKGPY+F PNVDLSKSL
Sbjct: 181 GLAALGRSNLSIPSVISAKFNSPKFFAICLSGARSGPGVAFFGSKGPYRFSPNVDLSKSL 240

Query: 241 IYTPLLFSPATNSIYTNWLPSYEYYIGLSAIRINGKAVPFNTSLLSFEPVIGGGGTKIST 300
            YTPLLF+PA+ SI+T WLPSYEYY+GLSAIRINGK VPFNTSLL FEP+ G GG KIST
Sbjct: 241 TYTPLLFNPASGSIHTYWLPSYEYYVGLSAIRINGKVVPFNTSLLPFEPIHGNGGAKIST 300

Query: 301 STNYALLQSSIYRAFTTVFMKESAALNFTLTNAVEPFGVCYTAYSVGVTAEGQARAPVVD 360
           STNY LL+SSIYRAF  VFMKE+AALNF L NAV+PFGVCY A SVGVTAEG A+APVVD
Sbjct: 301 STNYGLLESSIYRAFARVFMKEAAALNFKLINAVKPFGVCYAAKSVGVTAEGHAKAPVVD 360

Query: 361 LVMEKGKVVWKLGGRNTMVRIKKNGVDVWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 420
           LVMEKGKVVWKLGGRNTMVRIKK GVD WCLGFINGGEFPRTPIV+GGLQMEDHLLQFDL
Sbjct: 361 LVMEKGKVVWKLGGRNTMVRIKKKGVDAWCLGFINGGEFPRTPIVMGGLQMEDHLLQFDL 420

Query: 421 ENYRFGFSSSALMEGTSCSKFNFTSINN 448
           E +RFGFSSSAL EGTSCSKFNF SINN
Sbjct: 421 EKFRFGFSSSALTEGTSCSKFNFNSINN 448

BLAST of Bhi09G000855 vs. ExPASy TrEMBL
Match: A0A6J1H501 (basic 7S globulin 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460627 PE=4 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 2.4e-198
Identity = 354/449 (78.84%), Postives = 385/449 (85.75%), Query Frame = 0

Query: 1   MATPFFFFF--FFLLFFPLYSLQTAFVAPIYKDHISLLYSISVHLKTPLRPANLHLDLGG 60
           MATP FF F  F LL FP  SLQTAF+API+KDH S LYSISVHLKTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGG 60

Query: 61  GFSWIDCYNRYNSSSYQFVLCNSPLSHSLQQNTCGSCVEAPSPVCANDTIFSYAYPEKPF 120
            FSWIDCYN YNSSSY+ V CNSPLS S     CGSC++AP+P+CANDTIFSY YPEKP 
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPS 120

Query: 121 LRDQFVDYNHAKLTDSENVITDVLALFTTDGSDSYPLRRIPEIPFSCVKTDFLRGLARGV 180
           +RD++VDY +AKLTDSENV+TDVLAL TTDGS S  LRRI  +PF+CVKT+FLRGLAR V
Sbjct: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGRSNVSIPSVISAKFNSPRFFAICLSGARLGTGVAFFGSKGPYKFFPNVDLSKS 240
           IGLAALGR+N SIP  ISAKFNSPR FAICLSG RLG+GVAF GSKGPY F PNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKS 240

Query: 241 LIYTPLLFSPATNSIYTNWLPSYEYYIGLSAIRINGKAVPFNTSLLSFEPVIGGGGTKIS 300
           LIYTPLLF+P + SIYTNWLPSYEYYIGLSAIRIN +AV FNTSLL FEPV G GG KIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTNYALLQSSIYRAFTTVFMKESAALNFTLTNAVEPFGVCYTAYSVGVTAEGQARAPVV 360
           TST YALLQSSIYRAF   FMKE+A LNFTL NAVEPFGVC+   SV +TA G  RAPVV
Sbjct: 301 TSTTYALLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAG-PRAPVV 360

Query: 361 DLVMEKGKVVWKLGGRNTMVRIKKNGVDVWCLGFINGGEFPRTPIVIGGLQMEDHLLQFD 420
            L MEKGKVVWKLGGRN+MVRIKK GVD+WCLG++NGGEFPRTPIVIGGLQMEDHLLQFD
Sbjct: 361 YLEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFD 420

Query: 421 LENYRFGFSSSALMEGTSCSKFNFTSINN 448
           LE YRFGFSSSAL++GTSCSKFNF+S +N
Sbjct: 421 LEKYRFGFSSSALLQGTSCSKFNFSSSDN 448

BLAST of Bhi09G000855 vs. ExPASy TrEMBL
Match: A0A6J1KSD2 (basic 7S globulin 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497811 PE=4 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 9.1e-198
Identity = 351/449 (78.17%), Postives = 385/449 (85.75%), Query Frame = 0

Query: 1   MATPFFFFF--FFLLFFPLYSLQTAFVAPIYKDHISLLYSISVHLKTPLRPANLHLDLGG 60
           MATP FF F  F LL FP +SLQTAF+API+KDH SLLYSISVH+KTPLRPANLHLDLGG
Sbjct: 1   MATPLFFVFFTFSLLSFPFFSLQTAFIAPIHKDHNSLLYSISVHVKTPLRPANLHLDLGG 60

Query: 61  GFSWIDCYNRYNSSSYQFVLCNSPLSHSLQQNTCGSCVEAPSPVCANDTIFSYAYPEKPF 120
            FSWIDCYN YNSSSY+ V CNSPLS S     CGSC++AP+P C NDTIF+Y YPEKP 
Sbjct: 61  AFSWIDCYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPTCGNDTIFTYVYPEKPS 120

Query: 121 LRDQFVDYNHAKLTDSENVITDVLALFTTDGSDSYPLRRIPEIPFSCVKTDFLRGLARGV 180
           +RD++VDY +AKLTDSENV+TDVLAL TTDGS S  LR++  +PF+CVKT+FLRGLAR V
Sbjct: 121 IRDEYVDYLNAKLTDSENVVTDVLALSTTDGSRSGSLRQVSYMPFACVKTNFLRGLARNV 180

Query: 181 IGLAALGRSNVSIPSVISAKFNSPRFFAICLSGARLGTGVAFFGSKGPYKFFPNVDLSKS 240
           IGLAALGR+N SIP  ISAKFNSPR FAICLSG RLG+GVAFFGSKGPY F PNVDLSKS
Sbjct: 181 IGLAALGRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFFGSKGPYIFSPNVDLSKS 240

Query: 241 LIYTPLLFSPATNSIYTNWLPSYEYYIGLSAIRINGKAVPFNTSLLSFEPVIGGGGTKIS 300
           LIYTPLLF+P + SIYTNWLPSYEYYIGLSAIRIN + V FNTSLL FEPV G GG KIS
Sbjct: 241 LIYTPLLFNPQSGSIYTNWLPSYEYYIGLSAIRINNEVVRFNTSLLQFEPVHGRGGAKIS 300

Query: 301 TSTNYALLQSSIYRAFTTVFMKESAALNFTLTNAVEPFGVCYTAYSVGVTAEGQARAPVV 360
           TST YALLQSSIYRAF   FMKE+A LNFTL NAVEPFGVC+   SV +TA G  RAPVV
Sbjct: 301 TSTTYALLQSSIYRAFAMAFMKEAARLNFTLANAVEPFGVCFEGSSVEMTAAG-PRAPVV 360

Query: 361 DLVMEKGKVVWKLGGRNTMVRIKKNGVDVWCLGFINGGEFPRTPIVIGGLQMEDHLLQFD 420
            L MEKGKVVWKLGGRN+MVRIKK GVD+WCLG++NGGEFPRTPIVIGGLQMEDHLLQFD
Sbjct: 361 YLEMEKGKVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFD 420

Query: 421 LENYRFGFSSSALMEGTSCSKFNFTSINN 448
           LE YRFGFSSSALM+GTSCSKFNF+S +N
Sbjct: 421 LEKYRFGFSSSALMQGTSCSKFNFSSSDN 448

BLAST of Bhi09G000855 vs. ExPASy TrEMBL
Match: A0A1S3BRB7 (basic 7S globulin 2 OS=Cucumis melo OX=3656 GN=LOC103492684 PE=4 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 2.3e-156
Identity = 279/342 (81.58%), Postives = 300/342 (87.72%), Query Frame = 0

Query: 102 VCAND---TIFSYAYPEKPFLRDQFVDYNHAKLTDSENVITDVLALFTTDGSDSYPLRRI 161
           +C+ND   TIFS+ YPEKP +RDQFVDY H +LTDSE++ITDVLAL T DGS S  LRR 
Sbjct: 1   MCSNDTVNTIFSFGYPEKPSIRDQFVDYFHPELTDSESLITDVLALSTPDGSKSGLLRRT 60

Query: 162 PEIPFSCVKTDFLRGLARGVIGLAALGRSNVSIPSVISAKFNSPRFFAICLSGARLGTGV 221
           PE PF+CVKT+FLRGLA+ VIGLAALGRSN+SIPSVISAKFNSP+FFAICL GAR G GV
Sbjct: 61  PEFPFACVKTNFLRGLAKNVIGLAALGRSNLSIPSVISAKFNSPKFFAICLPGARSGPGV 120

Query: 222 AFFGSKGPYKFFPNVDLSKSLIYTPLLFSPATNSIYTNWLPSYEYYIGLSAIRINGKAVP 281
           A FGSKGPY+F PNVDLSKSL YTPL+F+P + SIYT WLPSYEYY+GLSAIRINGK VP
Sbjct: 121 AIFGSKGPYRFSPNVDLSKSLTYTPLVFNPVSGSIYTYWLPSYEYYVGLSAIRINGKVVP 180

Query: 282 FNTSLLSFEPVIGGGGTKISTSTNYALLQSSIYRAFTTVFMKESAALNFTLTNAVEPFGV 341
           FNTSLL FE + G GG KISTSTNY LLQSSIYRAF TVFMKE+A LNF L NAVEPFGV
Sbjct: 181 FNTSLLPFESIHGRGGAKISTSTNYGLLQSSIYRAFATVFMKEAAVLNFKLINAVEPFGV 240

Query: 342 CYTAYSVGVTAEGQARAPVVDLVMEKGKVVWKLGGRNTMVRIKKNGVDVWCLGFINGGEF 401
           CY A SVGVTAEG A+APVVDLVMEKGKVVWKLGGRNTMVRIKK GVD WCLGFINGGEF
Sbjct: 241 CYAAKSVGVTAEGHAKAPVVDLVMEKGKVVWKLGGRNTMVRIKKKGVDAWCLGFINGGEF 300

Query: 402 PRTPIVIGGLQMEDHLLQFDLENYRFGFSSSALMEGTSCSKF 441
           PRTPIVIGGLQMEDHLLQFDLE +RFGFSSSAL EGTSCSKF
Sbjct: 301 PRTPIVIGGLQMEDHLLQFDLEKFRFGFSSSALTEGTSCSKF 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G03220.11.1e-8340.98Eukaryotic aspartyl protease family protein [more]
AT1G03230.12.4e-8138.88Eukaryotic aspartyl protease family protein [more]
AT5G19110.11.5e-4028.67Eukaryotic aspartyl protease family protein [more]
AT5G19100.11.5e-3531.78Eukaryotic aspartyl protease family protein [more]
AT5G19120.11.4e-3329.75Eukaryotic aspartyl protease family protein [more]
Match NameE-valueIdentityDescription
I1JNS61.3e-8943.57Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2[more]
P0DO212.1e-7941.31Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 ... [more]
P829529.7e-5633.72Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2[more]
P139171.2e-4531.93Basic 7S globulin OS=Glycine max OX=3847 GN=BG PE=1 SV=2[more]
Q423691.2e-4530.65Gamma conglutin 1 OS=Lupinus angustifolius OX=3871 GN=LOC109345795 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K5062.5e-21182.66Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G39005... [more]
A0A5D3D4L47.1e-21182.14Basic 7S globulin 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2032... [more]
A0A6J1H5012.4e-19878.84basic 7S globulin 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460627 PE=4 SV=1[more]
A0A6J1KSD29.1e-19878.17basic 7S globulin 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497811 PE=4 SV=1[more]
A0A1S3BRB72.3e-15681.58basic 7S globulin 2 OS=Cucumis melo OX=3656 GN=LOC103492684 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 18..223
e-value: 1.7E-39
score: 137.9
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 225..444
e-value: 1.8E-57
score: 196.1
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 28..436
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 262..427
e-value: 2.5E-44
score: 150.9
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 37..223
e-value: 1.3E-23
score: 84.1
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47965ASPARTYL PROTEASE-RELATEDcoord: 6..442
NoneNo IPR availablePANTHERPTHR47965:SF6OS05G0403000 PROTEINcoord: 6..442
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 37..427
score: 18.60605

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi09M000855Bhi09M000855mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity