Bhi09G000828 (gene) Wax gourd (B227) v1

Overview
NameBhi09G000828
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionMethyl-CpG-binding domain-containing protein 4
Locationchr9: 22808802 .. 22811053 (+)
RNA-Seq ExpressionBhi09G000828
SyntenyBhi09G000828
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTAATACTTTGGTTATATCAGCAACAGAAAAATCTTTTAAAATTGTACTTTTAACAAAATATGAAGATATGATTCATTTAACAGCGGCTTTCCGCGGTCGGCAGAGGGAATTAGAGATCACGCCGCAGAGGGAGAGAGGGGGATCGTCGGGCGACATGGCCGCCGCCAGGCCTCCCAAAACGCCGGTGAAGGTACGACGGAAACTGTATCCTCTTCCTCCAATTTATTCTGTATATCCTCCTTAATTTCCCAATGATCTTGATCAGATTCAATTCTCTGTCATTTGGCTTCTCGCAAATTGTAACCCTTAAATTCGGAAGATTGGAAAATAGGTAACTAGAAAATTGATGCTGCTTAAGAAGTACTATGATCTTTCACGGTAACTAAGGAAAAATTATTGAATGTAGCTATAATAATCAAGGGTGCAAAACTATCGGTTACATTGTGGCAGTCTCCTGCAATAATTTCTCAGAAATAGGAGATTTGAACGAGATAATTACTTGAGTTTTGCTTAACTATGGAAACAGGCGGTGTAAGAGAACTTAATATTAGTTTGTAGGTCTAGCTCCAAGTATTTTGTGAGAATTAATGCTCATTTAAGCAGCAATTCAAACATAATCTTACAGTAATTCATCAACGTGCAAGTGTGCTAAAATATTTGTTGGCAGTGGCTGTGGGAAGTTATTTTGATATTCAGTCAGCTGTTCTGGGACACTCAATTTAATTCAGTTGGTTTTTAAGTGTGTGCCTTGCATATAGTCTTTTCTGTAAGTTTGTTTAACAAAGCCAAAAGGTTATTTGAAATCATATTATCATTTGGATGCCCAATTATCTTTCTAATGGAACAATTTGGACAGTGTTTCCAATTCTTGGAGTTAAAAGTTCGTCATAGGTTTAATGCTCATCTGTGAGCATTCTTCTCTAGGCATTTTTATACTTATGATATTGATCTTGTTCTTTTGGGCTCAAGTCCTTTTTGTAGTTGGTGTTGGCCTCAAGCTTTTTTAGGGCTTGATTTTTGTATGTCATTGTATTCTTTATTTTTTGTACTCACAAAGAAGTGAAAGTTAGAGGAGGAAGTGAATTTTCTTATTCTCATGTTTTAGCAATCAAATTTTAGAACTACAACTCCGTTCGATGATATAATATTAAATTTGTCTTCATTCATCAGCTTAAGTTTTTTGGTCAATCAGTGATTTAAGAAACTCATGTAGATTTTAATTTAGAACTTGCTAGGGGTCTTCATTTGTTGTTGCGTTGATTGTTGCATCTATAAATAAGAAATCCACATCTATAAATAAGAAATCCATGTAAGTTATTAGAAGCCTTTTTTGGTGTAGGCTAAGGTGTGCTATTGGTTTCTGTTTACAGTCAGCACGGCTGCCAGTAGGTCTATTTGCTGCACAATGTGAGGAATGCTTCAAATGGAGGTTGATAAGTACCCAAGAGGAGTACGAGGATATTAGAAGCAAGATGATAGAAGAACCATTTACTTGCCCTAGAAAAACCGATACTTCTTGTGACAATCCTCCCGATATCGACTATGATACAACTCGAACTTGGGGAATTGACAAACCCAACATCCCAAAGACACCACACGGTTTCACAAGACGCTTAGTGCTGAGAAAGAATTTCTCTAAATTTGATGCTTACTATGTTACACCCACAGGGAAGACAGTGAGATCCTCTACAGAGATGCTAGCTTTTGTAGAAGCAAATCCAGAATACAAGGATATACCTCTTTCAAGCTTCAGTTTTGCAGTCCCGAAAGTAATGGAAGAAACTGTACCTGAAAGCATTTTGAAAGAGGGCTTAAATAGCAGCAGTGCCAAAAGAATTAAGAAAACAAAGGATGAATCTGCCCATACCAACTGAAAATCAGTTAGGGGGTGTTTGGAGTTAGGAGTTGAGAAGTAGGAGTTTGTGAACTCCACTCCTTATTTAGCCTAAGGAATTTATGGGTCTCATTACTAAAAAACATCAATTTTATATCTTATCAACCTCTTATACTATAGGCCACGGGAGTTCAGAACTCCCTAGACTTCATAATTCCTTACTTTTCACTATTCCACTCATTATCTCAAACACCCCCTTAACGGTTTCCTTGCAGCATTCTTTTACTATTGTTGTTGATCTTAAATCCTTGTGAATGGTTCTAGACTTGAAAGATGTAGAATAGTGATTATCCTATTGTTTGTACCATTTTTTTTTTACAAGTAAGCACAAAGATAGATGCAGTCAAAGCTTATTG

mRNA sequence

TTTAATACTTTGGTTATATCAGCAACAGAAAAATCTTTTAAAATTGTACTTTTAACAAAATATGAAGATATGATTCATTTAACAGCGGCTTTCCGCGGTCGGCAGAGGGAATTAGAGATCACGCCGCAGAGGGAGAGAGGGGGATCGTCGGGCGACATGGCCGCCGCCAGGCCTCCCAAAACGCCGGTGAAGTCAGCACGGCTGCCAGTAGGTCTATTTGCTGCACAATGTGAGGAATGCTTCAAATGGAGGTTGATAAGTACCCAAGAGGAGTACGAGGATATTAGAAGCAAGATGATAGAAGAACCATTTACTTGCCCTAGAAAAACCGATACTTCTTGTGACAATCCTCCCGATATCGACTATGATACAACTCGAACTTGGGGAATTGACAAACCCAACATCCCAAAGACACCACACGGTTTCACAAGACGCTTAGTGCTGAGAAAGAATTTCTCTAAATTTGATGCTTACTATGTTACACCCACAGGGAAGACAGTGAGATCCTCTACAGAGATGCTAGCTTTTGTAGAAGCAAATCCAGAATACAAGGATATACCTCTTTCAAGCTTCAGTTTTGCAGTCCCGAAAGTAATGGAAGAAACTGTACCTGAAAGCATTTTGAAAGAGGGCTTAAATAGCAGCAGTGCCAAAAGAATTAAGAAAACAAAGGATGAATCTGCCCATACCAACTGAAAATCAGTTAGGGGGTGTTTGGAGTTAGGAGTTGAGAAGTAGGAGTTTGTGAACTCCACTCCTTATTTAGCCTAAGGAATTTATGGGTCTCATTACTAAAAAACATCAATTTTATATCTTATCAACCTCTTATACTATAGGCCACGGGAGTTCAGAACTCCCTAGACTTCATAATTCCTTACTTTTCACTATTCCACTCATTATCTCAAACACCCCCTTAACGGTTTCCTTGCAGCATTCTTTTACTATTGTTGTTGATCTTAAATCCTTGTGAATGGTTCTAGACTTGAAAGATGTAGAATAGTGATTATCCTATTGTTTGTACCATTTTTTTTTTACAAGTAAGCACAAAGATAGATGCAGTCAAAGCTTATTG

Coding sequence (CDS)

ATGATTCATTTAACAGCGGCTTTCCGCGGTCGGCAGAGGGAATTAGAGATCACGCCGCAGAGGGAGAGAGGGGGATCGTCGGGCGACATGGCCGCCGCCAGGCCTCCCAAAACGCCGGTGAAGTCAGCACGGCTGCCAGTAGGTCTATTTGCTGCACAATGTGAGGAATGCTTCAAATGGAGGTTGATAAGTACCCAAGAGGAGTACGAGGATATTAGAAGCAAGATGATAGAAGAACCATTTACTTGCCCTAGAAAAACCGATACTTCTTGTGACAATCCTCCCGATATCGACTATGATACAACTCGAACTTGGGGAATTGACAAACCCAACATCCCAAAGACACCACACGGTTTCACAAGACGCTTAGTGCTGAGAAAGAATTTCTCTAAATTTGATGCTTACTATGTTACACCCACAGGGAAGACAGTGAGATCCTCTACAGAGATGCTAGCTTTTGTAGAAGCAAATCCAGAATACAAGGATATACCTCTTTCAAGCTTCAGTTTTGCAGTCCCGAAAGTAATGGAAGAAACTGTACCTGAAAGCATTTTGAAAGAGGGCTTAAATAGCAGCAGTGCCAAAAGAATTAAGAAAACAAAGGATGAATCTGCCCATACCAACTGA

Protein sequence

MIHLTAAFRGRQRELEITPQRERGGSSGDMAAARPPKTPVKSARLPVGLFAAQCEECFKWRLISTQEEYEDIRSKMIEEPFTCPRKTDTSCDNPPDIDYDTTRTWGIDKPNIPKTPHGFTRRLVLRKNFSKFDAYYVTPTGKTVRSSTEMLAFVEANPEYKDIPLSSFSFAVPKVMEETVPESILKEGLNSSSAKRIKKTKDESAHTN
Homology
BLAST of Bhi09G000828 vs. TAIR 10
Match: AT3G63030.1 (methyl-CPG-binding domain 4 )

HSP 1 Score: 195.3 bits (495), Expect = 4.9e-50
Identity = 90/151 (59.60%), Postives = 112/151 (74.17%), Query Frame = 0

Query: 34  RPPKTPVKSARLP---VGLFAAQCEECFKWRLISTQEEYEDIRSKMIEEPFTCPRKTDTS 93
           +P K   K    P   +  +AAQC+ C KWR+I +QEEYEDIRSKM+E+PF C +K   S
Sbjct: 9   KPAKPKAKKDVAPGRLIDTYAAQCDNCHKWRVIDSQEEYEDIRSKMLEDPFNCQKKQGMS 68

Query: 94  CDNPPDIDYDTTRTWGIDKPNIPKTPHGFTRRLVLRKNFSKFDAYYVTPTGKTVRSSTEM 153
           C+ P DIDYD++RTW IDKP +PKTP GF R LVLRK++SK D YY TPTGK +RS  E+
Sbjct: 69  CEEPADIDYDSSRTWVIDKPGLPKTPKGFKRSLVLRKDYSKMDTYYFTPTGKKLRSRNEI 128

Query: 154 LAFVEANPEYKDIPLSSFSFAVPKVMEETVP 182
            AFVEANPE+++ PL  F+F VPKVME+TVP
Sbjct: 129 AAFVEANPEFRNAPLGDFNFTVPKVMEDTVP 159

BLAST of Bhi09G000828 vs. TAIR 10
Match: AT4G22745.1 (methyl-CPG-binding domain 1 )

HSP 1 Score: 181.8 bits (460), Expect = 5.6e-46
Identity = 88/180 (48.89%), Postives = 120/180 (66.67%), Query Frame = 0

Query: 16  EITPQRERGGSSGDMAAARPPKTPVKSARLPVGLFAAQCEECFKWRLISTQEEYEDIRSK 75
           +I  Q E+      +   +   TP +S    + +FA QCE+C KWR I TQ+EYEDIRS+
Sbjct: 25  KIEEQTEKSAEPTTIKVQKKAGTPGRS----IDVFAVQCEKCMKWRKIDTQDEYEDIRSR 84

Query: 76  MIEEPFTCPRKTDTSCDNPPDIDYDTTRTWGIDKPNIPKTPHGFTRRLVLRKNFSKFDAY 135
           + E+PF C  K   SC++  D++YD++RTW IDKP +P+TP GF R L+LRK++SK DAY
Sbjct: 85  VQEDPFFCKTKEGVSCEDVGDLNYDSSRTWVIDKPGLPRTPRGFKRSLILRKDYSKMDAY 144

Query: 136 YVTPTGKTVRSSTEMLAFVEANPEYKDIPLSSFSFAVPKVMEETVPESILKEGLNSSSAK 195
           Y+TPTGK ++S  E+ AF++AN +YK   L  F+F VPKVMEETVP  IL +     S K
Sbjct: 145 YITPTGKKLKSRNEIAAFIDANQDYKYALLGDFNFTVPKVMEETVPSGILSDRTPKPSRK 200

BLAST of Bhi09G000828 vs. TAIR 10
Match: AT5G35330.1 (methyl-CPG-binding domain protein 02 )

HSP 1 Score: 122.9 bits (307), Expect = 3.1e-28
Identity = 62/140 (44.29%), Postives = 89/140 (63.57%), Query Frame = 0

Query: 47  VGLFAAQCEECFKWRLISTQEEYEDIRSKMIEEPFTCPR----KTDTSCDNPPDIDYDTT 106
           +G F  QC  CFKWRL+ + ++YE+IR +++E PF C      K D SCD P DI  D T
Sbjct: 56  IGAFTVQCASCFKWRLMPSMQKYEEIREQLLENPFFCDTAREWKPDISCDVPADIYQDGT 115

Query: 107 RTWGIDKPNIPKTPHGFTRRLVLR-KNFSKF-DAYYVTPTGKTVRSSTEMLAFVEANPEY 166
           R W IDKPNI + P G+ R L +R +  ++F D YYV P+GK +RS+ E+  ++  N EY
Sbjct: 116 RLWAIDKPNISRPPAGWQRLLRIRGEGGTRFADVYYVAPSGKKLRSTVEVQKYLNDNSEY 175

Query: 167 --KDIPLSSFSFAVPKVMEE 179
             + + LS FSF +PK +++
Sbjct: 176 IGEGVKLSQFSFQIPKPLQD 195

BLAST of Bhi09G000828 vs. TAIR 10
Match: AT5G35330.2 (methyl-CPG-binding domain protein 02 )

HSP 1 Score: 122.9 bits (307), Expect = 3.1e-28
Identity = 62/140 (44.29%), Postives = 89/140 (63.57%), Query Frame = 0

Query: 47  VGLFAAQCEECFKWRLISTQEEYEDIRSKMIEEPFTCPR----KTDTSCDNPPDIDYDTT 106
           +G F  QC  CFKWRL+ + ++YE+IR +++E PF C      K D SCD P DI  D T
Sbjct: 56  IGAFTVQCASCFKWRLMPSMQKYEEIREQLLENPFFCDTAREWKPDISCDVPADIYQDGT 115

Query: 107 RTWGIDKPNIPKTPHGFTRRLVLR-KNFSKF-DAYYVTPTGKTVRSSTEMLAFVEANPEY 166
           R W IDKPNI + P G+ R L +R +  ++F D YYV P+GK +RS+ E+  ++  N EY
Sbjct: 116 RLWAIDKPNISRPPAGWQRLLRIRGEGGTRFADVYYVAPSGKKLRSTVEVQKYLNDNSEY 175

Query: 167 --KDIPLSSFSFAVPKVMEE 179
             + + LS FSF +PK +++
Sbjct: 176 IGEGVKLSQFSFQIPKPLQD 195

BLAST of Bhi09G000828 vs. TAIR 10
Match: AT5G35330.3 (methyl-CPG-binding domain protein 02 )

HSP 1 Score: 122.9 bits (307), Expect = 3.1e-28
Identity = 62/140 (44.29%), Postives = 89/140 (63.57%), Query Frame = 0

Query: 47  VGLFAAQCEECFKWRLISTQEEYEDIRSKMIEEPFTCPR----KTDTSCDNPPDIDYDTT 106
           +G F  QC  CFKWRL+ + ++YE+IR +++E PF C      K D SCD P DI  D T
Sbjct: 56  IGAFTVQCASCFKWRLMPSMQKYEEIREQLLENPFFCDTAREWKPDISCDVPADIYQDGT 115

Query: 107 RTWGIDKPNIPKTPHGFTRRLVLR-KNFSKF-DAYYVTPTGKTVRSSTEMLAFVEANPEY 166
           R W IDKPNI + P G+ R L +R +  ++F D YYV P+GK +RS+ E+  ++  N EY
Sbjct: 116 RLWAIDKPNISRPPAGWQRLLRIRGEGGTRFADVYYVAPSGKKLRSTVEVQKYLNDNSEY 175

Query: 167 --KDIPLSSFSFAVPKVMEE 179
             + + LS FSF +PK +++
Sbjct: 176 IGEGVKLSQFSFQIPKPLQD 195

BLAST of Bhi09G000828 vs. ExPASy Swiss-Prot
Match: Q9LYB9 (Methyl-CpG-binding domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=MBD4 PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 6.9e-49
Identity = 90/151 (59.60%), Postives = 112/151 (74.17%), Query Frame = 0

Query: 34  RPPKTPVKSARLP---VGLFAAQCEECFKWRLISTQEEYEDIRSKMIEEPFTCPRKTDTS 93
           +P K   K    P   +  +AAQC+ C KWR+I +QEEYEDIRSKM+E+PF C +K   S
Sbjct: 9   KPAKPKAKKDVAPGRLIDTYAAQCDNCHKWRVIDSQEEYEDIRSKMLEDPFNCQKKQGMS 68

Query: 94  CDNPPDIDYDTTRTWGIDKPNIPKTPHGFTRRLVLRKNFSKFDAYYVTPTGKTVRSSTEM 153
           C+ P DIDYD++RTW IDKP +PKTP GF R LVLRK++SK D YY TPTGK +RS  E+
Sbjct: 69  CEEPADIDYDSSRTWVIDKPGLPKTPKGFKRSLVLRKDYSKMDTYYFTPTGKKLRSRNEI 128

Query: 154 LAFVEANPEYKDIPLSSFSFAVPKVMEETVP 182
            AFVEANPE+++ PL  F+F VPKVME+TVP
Sbjct: 129 AAFVEANPEFRNAPLGDFNFTVPKVMEDTVP 159

BLAST of Bhi09G000828 vs. ExPASy Swiss-Prot
Match: Q5XEN5 (Methyl-CpG-binding domain-containing protein 1 OS=Arabidopsis thaliana OX=3702 GN=MBD1 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 7.9e-45
Identity = 88/180 (48.89%), Postives = 120/180 (66.67%), Query Frame = 0

Query: 16  EITPQRERGGSSGDMAAARPPKTPVKSARLPVGLFAAQCEECFKWRLISTQEEYEDIRSK 75
           +I  Q E+      +   +   TP +S    + +FA QCE+C KWR I TQ+EYEDIRS+
Sbjct: 25  KIEEQTEKSAEPTTIKVQKKAGTPGRS----IDVFAVQCEKCMKWRKIDTQDEYEDIRSR 84

Query: 76  MIEEPFTCPRKTDTSCDNPPDIDYDTTRTWGIDKPNIPKTPHGFTRRLVLRKNFSKFDAY 135
           + E+PF C  K   SC++  D++YD++RTW IDKP +P+TP GF R L+LRK++SK DAY
Sbjct: 85  VQEDPFFCKTKEGVSCEDVGDLNYDSSRTWVIDKPGLPRTPRGFKRSLILRKDYSKMDAY 144

Query: 136 YVTPTGKTVRSSTEMLAFVEANPEYKDIPLSSFSFAVPKVMEETVPESILKEGLNSSSAK 195
           Y+TPTGK ++S  E+ AF++AN +YK   L  F+F VPKVMEETVP  IL +     S K
Sbjct: 145 YITPTGKKLKSRNEIAAFIDANQDYKYALLGDFNFTVPKVMEETVPSGILSDRTPKPSRK 200

BLAST of Bhi09G000828 vs. ExPASy Swiss-Prot
Match: Q8LA53 (Methyl-CpG-binding domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=MBD2 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 4.4e-27
Identity = 62/140 (44.29%), Postives = 89/140 (63.57%), Query Frame = 0

Query: 47  VGLFAAQCEECFKWRLISTQEEYEDIRSKMIEEPFTCPR----KTDTSCDNPPDIDYDTT 106
           +G F  QC  CFKWRL+ + ++YE+IR +++E PF C      K D SCD P DI  D T
Sbjct: 56  IGAFTVQCASCFKWRLMPSMQKYEEIREQLLENPFFCDTAREWKPDISCDVPADIYQDGT 115

Query: 107 RTWGIDKPNIPKTPHGFTRRLVLR-KNFSKF-DAYYVTPTGKTVRSSTEMLAFVEANPEY 166
           R W IDKPNI + P G+ R L +R +  ++F D YYV P+GK +RS+ E+  ++  N EY
Sbjct: 116 RLWAIDKPNISRPPAGWQRLLRIRGEGGTRFADVYYVAPSGKKLRSTVEVQKYLNDNSEY 175

Query: 167 --KDIPLSSFSFAVPKVMEE 179
             + + LS FSF +PK +++
Sbjct: 176 IGEGVKLSQFSFQIPKPLQD 195

BLAST of Bhi09G000828 vs. ExPASy Swiss-Prot
Match: Q4PSK1 (Methyl-CpG-binding domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=MBD3 PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 5.2e-20
Identity = 61/152 (40.13%), Postives = 89/152 (58.55%), Query Frame = 0

Query: 50  FAAQCEECFKWRLISTQEEYEDIRSKMIEEPFTCPRKTDTSCDNPPD--IDYDT-TRTWG 109
           +AAQC +C K R I +QE+YE+IRSK +E+ F C R     C+ P D  +++D+ T  W 
Sbjct: 12  YAAQCWKCLKVRSIESQEDYEEIRSKTLEKFFECKR-----CEEPGDMVMNFDSLTMRWF 71

Query: 110 IDKPNIPKTPHGFTRRLVLRKNFSKFDAYY--VTPTGKTVRSSTEMLAFVEANPEYKDIP 169
            D+ +IPKTP G  R LV+R N  K D YY  + P  K  +S  E+  F+E   E+KD+ 
Sbjct: 72  QDEHSIPKTPQGLKRVLVVRTNCVKVDVYYESLAPRRKRFKSIKEVATFIEDKEEFKDMT 131

Query: 170 LSSFSFAVPKVMEETVPESILKEGLNSSSAKR 197
           L   SFA PK ++      + K+ ++S S+ R
Sbjct: 132 LEEVSFAAPKRLK------LKKKPVDSHSSSR 152

BLAST of Bhi09G000828 vs. ExPASy Swiss-Prot
Match: Q9FZP6 (Putative methyl-CpG-binding domain protein 12 OS=Arabidopsis thaliana OX=3702 GN=MBD12 PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.0e-15
Identity = 53/159 (33.33%), Postives = 86/159 (54.09%), Query Frame = 0

Query: 53  QCEECFKWRLISTQEEYEDIRSKMIEEPFTCPRKT----DTSCDNPPDIDYDTTRTWGID 112
           QC +C KWRLI + + Y  I+   ++ PF C   +    + SC+ P   D  T  TW   
Sbjct: 3   QCTDCKKWRLIPSMQHYNIIKETQLQTPFVCGTTSGWTPNMSCNVPQ--DGTTCDTW--- 62

Query: 113 KPNIPKTPHGFTRRLVLRKNFSKF-DAYYVTPTGKTVRSSTEMLAFVEANPEY--KDIPL 172
            P+IP  P G++R + +R   +KF D YY  P+G+ +RSS E+ +F++ +PEY  + +  
Sbjct: 63  -PSIPPIPTGWSRSVHIRSESTKFADVYYFPPSGERLRSSAEVQSFLDNHPEYVREGVNR 122

Query: 173 SSFSFAVPKVMEETVPESILKEGLNSSSAKRIKKTKDES 205
           S FSF +PK +++   +        +   KR K +KD +
Sbjct: 123 SQFSFQIPKPLDDNYVKK------RTRPVKRRKSSKDNN 149

BLAST of Bhi09G000828 vs. ExPASy TrEMBL
Match: A0A0A0K900 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G391290 PE=4 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 6.7e-87
Identity = 161/177 (90.96%), Postives = 170/177 (96.05%), Query Frame = 0

Query: 31  AAARPPKTPVKSARLPVGLFAAQCEECFKWRLISTQEEYEDIRSKMIEEPFTCPRKTDTS 90
           AA RPPKTP KS RLPVGLFAAQCEECFKWRLISTQEEYE IRSK+IEEPFTC RKTDTS
Sbjct: 4   AADRPPKTPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKTDTS 63

Query: 91  CDNPPDIDYDTTRTWGIDKPNIPKTPHGFTRRLVLRKNFSKFDAYYVTPTGKTVRSSTEM 150
           C++PPDIDYDTTRTWGIDKPNIPKTP GFTRRLVLRKNF+KFDAYYVTPTGKTVRSSTE+
Sbjct: 64  CEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSSTEV 123

Query: 151 LAFVEANPEYKDIPLSSFSFAVPKVMEETVPESILKEGLNSSSAKRIKKTKDESAHT 208
           LAFVEANP+YKDI LS+FSFAVPK+MEETVPESILKEGLNSSSAKRIKKTK+ESAHT
Sbjct: 124 LAFVEANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHT 180

BLAST of Bhi09G000828 vs. ExPASy TrEMBL
Match: A0A5A7UVI7 (Methyl-CpG-binding domain-containing protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G002230 PE=4 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 1.1e-86
Identity = 158/177 (89.27%), Postives = 172/177 (97.18%), Query Frame = 0

Query: 31  AAARPPKTPVKSARLPVGLFAAQCEECFKWRLISTQEEYEDIRSKMIEEPFTCPRKTDTS 90
           AAARPPKTP+KS R+PVGLFAAQCEECFKWRLISTQEEYEDIRS+MIEEPFTC R+TDTS
Sbjct: 3   AAARPPKTPIKSTRVPVGLFAAQCEECFKWRLISTQEEYEDIRSRMIEEPFTCQRRTDTS 62

Query: 91  CDNPPDIDYDTTRTWGIDKPNIPKTPHGFTRRLVLRKNFSKFDAYYVTPTGKTVRSSTEM 150
           C++PPDI+YDTTRTWGIDKPNIPKTP GFTRRLVLRKNF+KFDAYYVTPTGKTVRS TE+
Sbjct: 63  CEDPPDINYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSPTEI 122

Query: 151 LAFVEANPEYKDIPLSSFSFAVPKVMEETVPESILKEGLNSSSAKRIKKTKDESAHT 208
           LAFV+ANP+YKDI LS+FSFAVPK+MEETVPESILKEGLNSSSAKRIKKTK+ESAHT
Sbjct: 123 LAFVQANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHT 179

BLAST of Bhi09G000828 vs. ExPASy TrEMBL
Match: A0A1S3BRE2 (methyl-CpG-binding domain-containing protein 4 OS=Cucumis melo OX=3656 GN=LOC103492710 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.7e-85
Identity = 156/177 (88.14%), Postives = 170/177 (96.05%), Query Frame = 0

Query: 31  AAARPPKTPVKSARLPVGLFAAQCEECFKWRLISTQEEYEDIRSKMIEEPFTCPRKTDTS 90
           AAARPPKTP+KS R+PV LFA QCEECFKWRLISTQEEYEDIRS+MIEEPFTC RKTDTS
Sbjct: 3   AAARPPKTPIKSTRVPVSLFAVQCEECFKWRLISTQEEYEDIRSRMIEEPFTCQRKTDTS 62

Query: 91  CDNPPDIDYDTTRTWGIDKPNIPKTPHGFTRRLVLRKNFSKFDAYYVTPTGKTVRSSTEM 150
           C++PPDI+YDTTRTWGIDKPNIPKTP GFTRRLVLRKNF+KFDAYYVTPTGKTVRS TE+
Sbjct: 63  CEDPPDINYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSPTEI 122

Query: 151 LAFVEANPEYKDIPLSSFSFAVPKVMEETVPESILKEGLNSSSAKRIKKTKDESAHT 208
           LAFV+ANP+YKDI LS+FSFAVPK+MEETVPESILKEGLNSSSAKRIKKTK+ESA+T
Sbjct: 123 LAFVQANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAYT 179

BLAST of Bhi09G000828 vs. ExPASy TrEMBL
Match: A0A6J1D952 (methyl-CpG-binding domain-containing protein 4-like OS=Momordica charantia OX=3673 GN=LOC111018404 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.6e-83
Identity = 156/195 (80.00%), Postives = 175/195 (89.74%), Query Frame = 0

Query: 14  ELEITPQRERGGSSGDMAAARPPKTPVKSARLPVGLFAAQCEECFKWRLISTQEEYEDIR 73
           ++EI  +  +  SSG+MA ARPP+ PVKS RLPV L+AAQCEECFKWRLISTQEEYEDIR
Sbjct: 10  QVEIGDRSRKRESSGEMAGARPPRKPVKSKRLPVDLYAAQCEECFKWRLISTQEEYEDIR 69

Query: 74  SKMIEEPFTCPRKTDTSCDNPPDIDYDTTRTWGIDKPNIPKTPHGFTRRLVLRKNFSKFD 133
           SK+IEEPFTC RKTDTSC++  DIDYD TRTW IDKPNIPKTP GF RRLVLRK+FSKFD
Sbjct: 70  SKLIEEPFTCNRKTDTSCEDASDIDYDATRTWVIDKPNIPKTPEGFKRRLVLRKDFSKFD 129

Query: 134 AYYVTPTGKTVRSSTEMLAFVEANPEYKDIPLSSFSFAVPKVMEETVPESILKEGLNSSS 193
           AYY+TPTGKTVRSSTEML+F+EANP+YKDIPLS FSFAVPKVMEETVPESIL+E L+SSS
Sbjct: 130 AYYITPTGKTVRSSTEMLSFIEANPKYKDIPLSKFSFAVPKVMEETVPESILREDLSSSS 189

Query: 194 AKRIKKTKDESAHTN 209
           AKRIKK KD+SAHT+
Sbjct: 190 AKRIKKVKDKSAHTD 204

BLAST of Bhi09G000828 vs. ExPASy TrEMBL
Match: A0A6J1GNJ9 (methyl-CpG-binding domain-containing protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC111456046 PE=4 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 3.2e-81
Identity = 149/179 (83.24%), Postives = 165/179 (92.18%), Query Frame = 0

Query: 30  MAAARPPKTPVKSARLPVGLFAAQCEECFKWRLISTQEEYEDIRSKMIEEPFTCPRKTDT 89
           MAAARP +TP+KS RLPVGL+AAQCEECFKWRLISTQEEYEDIRSK+IEEPFTC RKTDT
Sbjct: 1   MAAARPTRTPMKSTRLPVGLYAAQCEECFKWRLISTQEEYEDIRSKLIEEPFTCHRKTDT 60

Query: 90  SCDNPPDIDYDTTRTWGIDKPNIPKTPHGFTRRLVLRKNFSKFDAYYVTPTGKTVRSSTE 149
           SC +PPDI+YD TRTWGIDKPNIPKTP GFTR LV+RKNFSKFDAYYVTPTGKTVRS TE
Sbjct: 61  SCKDPPDIEYDATRTWGIDKPNIPKTPEGFTRHLVVRKNFSKFDAYYVTPTGKTVRSPTE 120

Query: 150 MLAFVEANPEYKDIPLSSFSFAVPKVMEETVPESILKEGLNSSSAKRIKKTKDESAHTN 209
           M AF+ ANP +KD+PL+ FSF +P++MEETVPESILKEGL+SSSAKRIKKTKDE AHT+
Sbjct: 121 MQAFLGANPRHKDVPLTDFSFTIPRLMEETVPESILKEGLDSSSAKRIKKTKDEFAHTD 179

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G63030.14.9e-5059.60methyl-CPG-binding domain 4 [more]
AT4G22745.15.6e-4648.89methyl-CPG-binding domain 1 [more]
AT5G35330.13.1e-2844.29methyl-CPG-binding domain protein 02 [more]
AT5G35330.23.1e-2844.29methyl-CPG-binding domain protein 02 [more]
AT5G35330.33.1e-2844.29methyl-CPG-binding domain protein 02 [more]
Match NameE-valueIdentityDescription
Q9LYB96.9e-4959.60Methyl-CpG-binding domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 G... [more]
Q5XEN57.9e-4548.89Methyl-CpG-binding domain-containing protein 1 OS=Arabidopsis thaliana OX=3702 G... [more]
Q8LA534.4e-2744.29Methyl-CpG-binding domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 G... [more]
Q4PSK15.2e-2040.13Methyl-CpG-binding domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 G... [more]
Q9FZP61.0e-1533.33Putative methyl-CpG-binding domain protein 12 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A0A0K9006.7e-8790.96Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G391290 PE=4 SV=1[more]
A0A5A7UVI71.1e-8689.27Methyl-CpG-binding domain-containing protein 4 OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3BRE23.7e-8588.14methyl-CpG-binding domain-containing protein 4 OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1D9522.6e-8380.00methyl-CpG-binding domain-containing protein 4-like OS=Momordica charantia OX=36... [more]
A0A6J1GNJ93.2e-8183.24methyl-CpG-binding domain-containing protein 4-like OS=Cucurbita moschata OX=366... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001739Methyl-CpG DNA bindingSMARTSM00391TAM_2coord: 107..180
e-value: 7.5E-12
score: 55.4
IPR001739Methyl-CpG DNA bindingPFAMPF01429MBDcoord: 114..176
e-value: 9.4E-11
score: 41.3
IPR001739Methyl-CpG DNA bindingPROSITEPS50982MBDcoord: 105..175
score: 13.540434
IPR011124Zinc finger, CW-typePFAMPF07496zf-CWcoord: 51..96
e-value: 6.1E-10
score: 38.9
IPR011124Zinc finger, CW-typePROSITEPS51050ZF_CWcoord: 44..99
score: 11.500962
NoneNo IPR availableGENE3D3.30.890.10coord: 98..208
e-value: 5.1E-14
score: 54.3
NoneNo IPR availableGENE3D3.30.40.100coord: 50..97
e-value: 3.2E-5
score: 25.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 187..208
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 9..39
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 192..208
NoneNo IPR availablePANTHERPTHR12396:SF10METHYL-CPG-BINDING DOMAIN-CONTAINING PROTEIN 1-RELATEDcoord: 37..202
NoneNo IPR availablePANTHERPTHR12396METHYL-CPG BINDING PROTEIN, MBDcoord: 37..202
NoneNo IPR availableCDDcd01396MeCP2_MBDcoord: 109..181
e-value: 3.29511E-19
score: 76.2573
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 86..180

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi09M000828Bhi09M000828mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0008270 zinc ion binding