Bhi09G000717 (gene) Wax gourd (B227) v1

Overview


Name	Bhi09G000717
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	pectinesterase inhibitor 6
Location	chr9: 19759267 .. 19761537 (+)
RNA-Seq Expression	Bhi09G000717
Synteny	Bhi09G000717

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCCTATTGATAAGTTTGGTAGTTAATTATCTAATCTTGAAACCGATGATTAAAAAGCACGTTGAAACCGGAAAAAAAAAAAATGAAAATACAGAGTTTATGAAACTAAAATAATATATGGGTGCTGAGTGCTTGCCAAACTCTTACTAATGGCAACTCCATTCTCAACTTCAAGCTGGCAAATGTTGACATCCAAAGCTTGGACCTTCCTCGCTCGAATTAGCTCATAATGAAATGAAGGTTGAACCAAACCTATCAAAATTTCTAGATGAAAAATGAATGCTTGCCAAATCTTCTTGGCCCTGGATCTTGTAATAGGTCCAATTGAAACTAGTGGAACGCCCATTATCCTTATCACTCAAGCTAATTACAAATAAGTACAATTATGTAATATGATAAGAGAGAATTTTCTGTTTTCCCGTAGTCAAGTAAATAATTTTCCCCAAGAACTTCTTTTTGGTTAGAAGTCCAATCCATGTACAGAAATCTCAAGATCAACTAGTCAAAGACGTATAGTAAATTCTGGTAAAACAGATACCAAATTTTCCAAGATTTTCTTTATCCCAAAGTTCATGAGAACTCACCTGCCATTCACATGTTCTTCACCTTCTCAACTTATTTCACTTCATCACTACTTCTCCTCCCCTTCTTCAATGCATTCACCATCCCCACGCCTAATGTATAGCAAATACCTGCAAAAAGTTATTTAAAATGAACTTTTCAAGCCACCCCAAATACCCTTTTCCCCACTATATAGTCCACCCATAATCTCATTAATCACCACATTAATTTCAATCTGATTCATAAACCACAGCAGTATAAGCTGATTTCATAGAAATTCATCTCCCATTTCCACATACCTTTTTTTCCACTTCTTTCAGACATTTCATCGAACATGTTGCGTGCACCCAATACGAGAGCATCAATCGCAACCTTTTTGGCCCTCATCTCCATTCTCCCAAGGCTAACCCACTCCACCAAAACGAGCTATGTCCGAGAAGCCTGCAGCGTAACACGACACCAAGATCTCTGCATCCAATCCCTGACGCCATTTTCAAGCACCGCCAAGCGAAGCCCTGCAAAATGGGCGCGAGCGGGGGTGTCAGTGACGATAACAGAGGCGAAAACCGTGGCAGGATTGTTGGGGAGGTTGAAGAACAACAAGCGGATGAAGGGGAGAAACAGGGCTGCAGTTTCGGACTGTGTGGAGGTCTTTGAAGCCGCCATTGATGAGCTCCATCGGTCACTTGGGGTTCTTAGAAGACTGAGTAGGAGCAATTTTGATGCACAGATAGGGGATCTGACGACGTGGGTTAGCGCTGCGCTGACGGATGAAGATACTTGTGTTGAAGGATTTGAAGGAGAAAGGGGAAAATTAGTGAATTTGATAAGGAACCGTGTGTTGAAGGTTGGTTATATTACGAGTAACGCACTTGCTTTGGTTAACAAATTGGCTGCCTCTGGACTCGAAACTGCACCAAATCAGTAAAAATGACATCGGGATTTCTCGAATGTGAAGTGGAGACGGTATACATATGATAATTTGTAATTGTACCCTTTGAGAACTTTGCATTTTGGTGTTTCACAAGCTTTTTTTCCCCTTAAATATGATGAAGTCACTTTTAATCCTTTTTGTTCTTTTGAAGTTCAACAACTTTTTCAATCCAACAAATCTAACACAGAGAATAGTTTATTAAAAGTAGGTTTGCTTTGAAAGAAGATGAGCCTAGAAGTCGTTTCAACAGATGTCATTCAACTGCAATATAGATTTAAGAGATACTAAAAGTCAAAAACGATTTAACTCAGTTAACTGGGTGATAATAGAAATTGAATAATGGCATTTAATTAGCAGCCACCATTGACTAACATCTCATCAAATAAGTAATTTAGAGAACTAAATTTGGAGGGGTGCGCCAGGCCCAAGCAATAGTGCAACGCATGGGCGACGCTCGAAGGGCCCAATAGCAAGCTATTGTGGTGGACCTTCCCCCTTGAAAAATAAATTTAATATCATGTGGGCCGATGGCCCACGTATCAGATATTAAACTGATAAGAACAGATACTACACTTGATCTTAGCCAAAAGGCCGAGAAAGGTATGCTTATCAAAGCCTTACACCACCGCTTTTATACTCACCTTCTCCCTCCACCTTCGTCAAAATAATCGATGTGGGAATAAAGCTGCACGTACACCTCGTATTGTTTTTCAGAAGCTCTGCTTTTCTTTTTTACTTGGCCAGTATCAAACCGATTATTATATCGACTCTTTTTG

mRNA sequence

CCCCTATTGATAAGTTTGGTAGTTAATTATCTAATCTTGAAACCGATGATTAAAAAGCACGTTGAAACCGGAAAAAAAAAAAATGAAAATACAGAGTTTATGAAACTAAAATAATATATGGGTGCTGAGTGCTTGCCAAACTCTTACTAATGGCAACTCCATTCTCAACTTCAAGCTGGCAAATGTTGACATCCAAAGCTTGGACCTTCCTCGCTCGAATTAGCTCATAATGAAATGAAGGTTGAACCAAACCTATCAAAATTTCTAGATGAAAAATGAATGCTTGCCAAATCTTCTTGGCCCTGGATCTTGTAATAGGTCCAATTGAAACTAGTGGAACGCCCATTATCCTTATCACTCAAGCTAATTACAAATAAGTACAATTATGTAATATGATAAGAGAGAATTTTCTGTTTTCCCGTAGTCAAGTAAATAATTTTCCCCAAGAACTTCTTTTTGGTTAGAAGTCCAATCCATGTACAGAAATCTCAAGATCAACTAGTCAAAGACGTATAGTAAATTCTGGTAAAACAGATACCAAATTTTCCAAGATTTTCTTTATCCCAAAGTTCATGAGAACTCACCTGCCATTCACATGTTCTTCACCTTCTCAACTTATTTCACTTCATCACTACTTCTCCTCCCCTTCTTCAATGCATTCACCATCCCCACGCCTAATGTATAGCAAATACCTGCAAAAAGTTATTTAAAATGAACTTTTCAAGCCACCCCAAATACCCTTTTCCCCACTATATAGTCCACCCATAATCTCATTAATCACCACATTAATTTCAATCTGATTCATAAACCACAGCAGTATAAGCTGATTTCATAGAAATTCATCTCCCATTTCCACATACCTTTTTTTCCACTTCTTTCAGACATTTCATCGAACATGTTGCGTGCACCCAATACGAGAGCATCAATCGCAACCTTTTTGGCCCTCATCTCCATTCTCCCAAGGCTAACCCACTCCACCAAAACGAGCTATGTCCGAGAAGCCTGCAGCGTAACACGACACCAAGATCTCTGCATCCAATCCCTGACGCCATTTTCAAGCACCGCCAAGCGAAGCCCTGCAAAATGGGCGCGAGCGGGGGTGTCAGTGACGATAACAGAGGCGAAAACCGTGGCAGGATTGTTGGGGAGGTTGAAGAACAACAAGCGGATGAAGGGGAGAAACAGGGCTGCAGTTTCGGACTGTGTGGAGGTCTTTGAAGCCGCCATTGATGAGCTCCATCGGTCACTTGGGGTTCTTAGAAGACTGAGTAGGAGCAATTTTGATGCACAGATAGGGGATCTGACGACGTGGGTTAGCGCTGCGCTGACGGATGAAGATACTTGTGTTGAAGGATTTGAAGGAGAAAGGGGAAAATTAGTGAATTTGATAAGGAACCGTGTGTTGAAGGTTGGTTATATTACGAGTAACGCACTTGCTTTGGTTAACAAATTGGCTGCCTCTGGACTCGAAACTGCACCAAATCAGTAAAAATGACATCGGGATTTCTCGAATGTGAAGTGGAGACGGTATACATATGATAATTTGTAATTGTACCCTTTGAGAACTTTGCATTTTGGTGTTTCACAAGCTTTTTTTCCCCTTAAATATGATGAAGTCACTTTTAATCCTTTTTGTTCTTTTGAAGTTCAACAACTTTTTCAATCCAACAAATCTAACACAGAGAATAGTTTATTAAAAGTAGGTTTGCTTTGAAAGAAGATGAGCCTAGAAGTCGTTTCAACAGATGTCATTCAACTGCAATATAGATTTAAGAGATACTAAAAGTCAAAAACGATTTAACTCAGTTAACTGGGTGATAATAGAAATTGAATAATGGCATTTAATTAGCAGCCACCATTGACTAACATCTCATCAAATAAGTAATTTAGAGAACTAAATTTGGAGGGGTGCGCCAGGCCCAAGCAATAGTGCAACGCATGGGCGACGCTCGAAGGGCCCAATAGCAAGCTATTGTGGTGGACCTTCCCCCTTGAAAAATAAATTTAATATCATGTGGGCCGATGGCCCACGTATCAGATATTAAACTGATAAGAACAGATACTACACTTGATCTTAGCCAAAAGGCCGAGAAAGGTATGCTTATCAAAGCCTTACACCACCGCTTTTATACTCACCTTCTCCCTCCACCTTCGTCAAAATAATCGATGTGGGAATAAAGCTGCACGTACACCTCGTATTGTTTTTCAGAAGCTCTGCTTTTCTTTTTTACTTGGCCAGTATCAAACCGATTATTATATCGACTCTTTTTG

Coding sequence (CDS)

ATGTTGCGTGCACCCAATACGAGAGCATCAATCGCAACCTTTTTGGCCCTCATCTCCATTCTCCCAAGGCTAACCCACTCCACCAAAACGAGCTATGTCCGAGAAGCCTGCAGCGTAACACGACACCAAGATCTCTGCATCCAATCCCTGACGCCATTTTCAAGCACCGCCAAGCGAAGCCCTGCAAAATGGGCGCGAGCGGGGGTGTCAGTGACGATAACAGAGGCGAAAACCGTGGCAGGATTGTTGGGGAGGTTGAAGAACAACAAGCGGATGAAGGGGAGAAACAGGGCTGCAGTTTCGGACTGTGTGGAGGTCTTTGAAGCCGCCATTGATGAGCTCCATCGGTCACTTGGGGTTCTTAGAAGACTGAGTAGGAGCAATTTTGATGCACAGATAGGGGATCTGACGACGTGGGTTAGCGCTGCGCTGACGGATGAAGATACTTGTGTTGAAGGATTTGAAGGAGAAAGGGGAAAATTAGTGAATTTGATAAGGAACCGTGTGTTGAAGGTTGGTTATATTACGAGTAACGCACTTGCTTTGGTTAACAAATTGGCTGCCTCTGGACTCGAAACTGCACCAAATCAGTAA

Protein sequence

MLRAPNTRASIATFLALISILPRLTHSTKTSYVREACSVTRHQDLCIQSLTPFSSTAKRSPAKWARAGVSVTITEAKTVAGLLGRLKNNKRMKGRNRAAVSDCVEVFEAAIDELHRSLGVLRRLSRSNFDAQIGDLTTWVSAALTDEDTCVEGFEGERGKLVNLIRNRVLKVGYITSNALALVNKLAASGLETAPNQ

Homology

[Close]

BLAST of Bhi09G000717 vs. TAIR 10
Match: AT3G62820.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 162.5 bits (410), Expect = 3.4e-40
Identity = 92/186 (49.46%), Postives = 127/186 (68.28%), Query Frame = 0


Query: 10  SIATFLALISILPRLT-HSTKTSYVREACSVTRHQDLCIQSLTPFSSTAKRSPAKWARAG 69
           + A    L+S+ P  +  S + SYV+ ACSVTR+QDLC ++L PF+S AK SP+KWARAG
Sbjct: 10  TFALVFFLLSLNPTSSLPSKRESYVQNACSVTRYQDLCAKTLLPFASVAKNSPSKWARAG 69

Query: 70  VSVTITEAKTVAGLLGRLKNNKRMKGRNRAAVSDCVEVFEAAIDELHRSLGVLRRLSRSN 129
           VSV IT+ K V   L + + +   K R+R A+SDC E+ + ++D LH+SL VLR L  S 
Sbjct: 70  VSVAITDNKDVLRHLLKTRLSTIGK-RDRIALSDCRELLQDSLDSLHKSLAVLRTLRASE 129

Query: 130 FDAQIGDLTTWVSAALTDEDTCVEGFE---GERGKLVNLIRNRVLKVGYITSNALALVNK 189
           F  Q+ DL TW+S++LTD+DTC++GFE         V +IR RV    Y++SN+LAL+NK
Sbjct: 130 FQQQMSDLATWLSSSLTDKDTCLDGFEKTSTRSSSTVRMIRKRVTTSMYLSSNSLALLNK 189

Query: 190 LAASGL 192
           LAA+GL
Sbjct: 190 LAANGL 194

[Close]

BLAST of Bhi09G000717 vs. TAIR 10
Match: AT2G47670.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 149.1 bits (375), Expect = 3.8e-36
Identity = 80/165 (48.48%), Postives = 112/165 (67.88%), Query Frame = 0


Query: 30  TSYVREACSVTRHQDLCIQSLTPFSSTAKRSPAKWARAGVSVTITEAKTVAGLLGRLKNN 89
           ++YVR AC+VTR+  LC+++L PF+  A+ + +KWARA V+VTIT+ K V  LL  LK  
Sbjct: 46  STYVRNACNVTRYNRLCVRTLWPFAIVARNNTSKWARASVAVTITDTKRVLRLL--LKTQ 105

Query: 90  KRMKGRN-RAAVSDCVEVFEAAIDELHRSLGVLRRLSRSNFDAQIGDLTTWVSAALTDED 149
           +   G + R A+SDC E+F  ++D L++SL VLR L+   F  QI DL TW+SAALTD+D
Sbjct: 106 RSAVGESERIALSDCRELFVDSLDNLYKSLAVLRTLNADEFQRQISDLATWLSAALTDDD 165

Query: 150 TCVEGFE--GERGKLVNLIRNRVLKVGYITSNALALVNKLAASGL 192
           TC++GFE    R + V ++R +  K   + SNALAL+ KLA  GL
Sbjct: 166 TCLDGFEETSSRTRTVRMVRRKATKCMRLCSNALALLKKLAFDGL 208

[Close]

BLAST of Bhi09G000717 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 136.3 bits (342), Expect = 2.6e-32
Identity = 74/188 (39.36%), Postives = 119/188 (63.30%), Query Frame = 0


Query: 11  IATFLALISILPRL--------THSTKTSYVREACSVTRHQDLCIQSLTPFSSTAKRSPA 70
           + TFL +I++L  +        T +T T +V+ +C+ T +  LC  SL+  +S  + SP 
Sbjct: 14  LTTFLIIIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPK 73

Query: 71  KWARAGVSVTITEAKTVAGLLGRLKNNKRMKGRNRAAVSDCVEVFEAAIDELHRSLGVLR 130
             A A +++T+  AK  + ++ RL +N R+K +  +A+ DCVE     ++EL +S+G + 
Sbjct: 74  LMAHAALNITLASAKVTSAMMVRL-SNSRLKPKEVSAMRDCVEELGDTLEELRKSIGEMC 133

Query: 131 RLSRSNFDAQIGDLTTWVSAALTDEDTCVEGFEGE--RGKLVNLIRNRVLKVGYITSNAL 189
           +LS SN++  I D+ TWVSAALTD +TC +GFEGE   GK+  L+R R+L + ++TSNAL
Sbjct: 134 QLSGSNYEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNAL 193

[Close]

BLAST of Bhi09G000717 vs. TAIR 10
Match: AT4G12390.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 115.9 bits (289), Expect = 3.6e-26
Identity = 63/169 (37.28%), Postives = 105/169 (62.13%), Query Frame = 0


Query: 27  STKTSYVREACSVTRHQDLCIQSLTPFSSTAKRSPAKWARAGVSVTITEAKTVAGLLGRL 86
           S+  +++  +C VTR+Q LC++ L  F+   +R+  + A+  ++VT+   ++    +G+L
Sbjct: 34  SSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNENQLAQTALAVTLVRVQSTTIYVGKL 93

Query: 87  KNNKRMKGRNRAAVSDCVEVFEAAIDELHRSLGVLRRLSRSNFDA-----QIGDLTTWVS 146
              +R+K R   AV DCVE     ++ L +S+  L+++ RS  D      ++ ++ TWVS
Sbjct: 94  TKARRIKRREYLAVKDCVENLGDGLEMLAQSMRELKQVGRSGRDRDEFLWRLSNVETWVS 153

Query: 147 AALTDEDTCVEGFEGE--RGKLVNLIRNRVLKVGYITSNALALVNKLAA 189
           AALTDE TC++GF+G+   G + + IR RV+ V  +TSNALALVN+ AA
Sbjct: 154 AALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARVTSNALALVNRFAA 202

[Close]

BLAST of Bhi09G000717 vs. TAIR 10
Match: AT2G01610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 109.4 bits (272), Expect = 3.4e-24
Identity = 68/196 (34.69%), Postives = 109/196 (55.61%), Query Frame = 0


Query: 15  LALISILPR--LTHSTKTSYVREACSVTRHQDLCIQSLTPFSSTAKRSPAKWARAGVSVT 74
           ++ + +LPR   T +    ++R +C+ T + D+C  SL+ ++S  + SPA+ A+  + V+
Sbjct: 25  ISAVRLLPRPNTTTTNDLDFIRTSCNATLYPDVCFTSLSGYASAVQDSPARLAKLAIGVS 84

Query: 75  ITEAKTVAGLLGRLKNNKRMKGRN-----RAAVSDCVEVFEAAIDELHRSLGVLR----- 134
           +++AK+ A  L +L  +      +      A + DCV   E A+DE+  SL  LR     
Sbjct: 85  LSQAKSTAAFLSKLSRSAAKYSGDGHQTASAVIRDCVSNVEDAVDEMRGSLRQLRDMNGR 144

Query: 135 ------RLSRSNFDAQIGDLTTWVSAALTDEDTCVEGFE--GERGKLVNLIRNRVLKVGY 191
                 R S   F  Q+ ++ TW+SAALTDEDTC +GFE   E G +   + +R+ +V  
Sbjct: 145 GGGTAARRSVETFRFQMSNVQTWMSAALTDEDTCTDGFEDMDEGGLIKTTVCDRLEEVKR 204

[Close]

BLAST of Bhi09G000717 vs. ExPASy Swiss-Prot
Match: O22244 (Pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PMEI6 PE=2 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 5.4e-35
Identity = 80/165 (48.48%), Postives = 112/165 (67.88%), Query Frame = 0


Query: 30  TSYVREACSVTRHQDLCIQSLTPFSSTAKRSPAKWARAGVSVTITEAKTVAGLLGRLKNN 89
           ++YVR AC+VTR+  LC+++L PF+  A+ + +KWARA V+VTIT+ K V  LL  LK  
Sbjct: 46  STYVRNACNVTRYNRLCVRTLWPFAIVARNNTSKWARASVAVTITDTKRVLRLL--LKTQ 105

Query: 90  KRMKGRN-RAAVSDCVEVFEAAIDELHRSLGVLRRLSRSNFDAQIGDLTTWVSAALTDED 149
           +   G + R A+SDC E+F  ++D L++SL VLR L+   F  QI DL TW+SAALTD+D
Sbjct: 106 RSAVGESERIALSDCRELFVDSLDNLYKSLAVLRTLNADEFQRQISDLATWLSAALTDDD 165

Query: 150 TCVEGFE--GERGKLVNLIRNRVLKVGYITSNALALVNKLAASGL 192
           TC++GFE    R + V ++R +  K   + SNALAL+ KLA  GL
Sbjct: 166 TCLDGFEETSSRTRTVRMVRRKATKCMRLCSNALALLKKLAFDGL 208

[Close]

BLAST of Bhi09G000717 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.7e-25
Identity = 65/184 (35.33%), Postives = 109/184 (59.24%), Query Frame = 0


Query: 10  SIATFLALISILPRLTHSTKTSYVREACSVTRHQDLCIQSLTPFSSTAKRSPAKWARAGV 69
           S   F AL+ IL   + +    +++ +C++T +  +C QSL+ ++ T + +P + A   +
Sbjct: 6   STLVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTAL 65

Query: 70  SVTITEAKTVAGLLGRLKNNKRMKGRNRAAVSDCVEVFEAAIDELHRSLGVLRRLSR--- 129
            V++T  +     + RL   K +K R  AA+ DC+E  E ++D + RS   ++ LS    
Sbjct: 66  QVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKG 125

Query: 130 SNFDAQIGDLTTWVSAALTDEDTCVEGFEGE--RGKLVNLIRNRVLKVGYITSNALALVN 189
           ++F  ++ ++ TWVSAALTDE TC++GF G+   GK+   +R +V+ V  +TSNALALVN
Sbjct: 126 NDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVN 185

[Close]

BLAST of Bhi09G000717 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.1e-22
Identity = 61/168 (36.31%), Postives = 95/168 (56.55%), Query Frame = 0


Query: 27  STKTSYVREACSVTRHQDLCIQSLTPFSSTAKRSPAKWARAGVSVTITEAKTVAGLLGRL 86
           S  T +++ +C  T + D C QSL+ ++S  K+ P K A   ++V+I  AK+    +  +
Sbjct: 32  SGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEM 91

Query: 87  KNNKRMKGRNRAAVSDCVEVFEAAIDELHRSLGVLRRL----SRSNFDAQIGDLTTWVSA 146
            + K +  R   AV+DC+E     +D L  SL  L+ L    S  +F   + ++ TW SA
Sbjct: 92  TDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSA 151

Query: 147 ALTDEDTCVEGFEGE--RGKLVNLIRNRVLKVGYITSNALALVNKLAA 189
           ALTDE  C++GF G+   G+L +LIR  ++ V   TSNALAL+N  A+
Sbjct: 152 ALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNALALINDFAS 199

[Close]

BLAST of Bhi09G000717 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.4e-22
Identity = 53/168 (31.55%), Postives = 101/168 (60.12%), Query Frame = 0


Query: 25  THSTKTSYVREACSVTRHQDLCIQSLTPFSSTAKRSPAKWARAGVSVTITEAKTVAGLLG 84
           ++ T   Y++ +C++T ++ +C  SL+P++ST + +P K A   +++T++ AK+ +  + 
Sbjct: 140 SNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVK 199

Query: 85  RLKNNKRMKGRNRAAVSDCVEVFEAAIDELH---RSLGVLRRLSRSNFDAQIGDLTTWVS 144
            + +   +      AV+DCVE    ++  L    R L  +     + F+  + D+ TWVS
Sbjct: 200 NISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVS 259

Query: 145 AALTDEDTCVEGFEGERGKLVNLIRNRVLKVGYITSNALALVNKLAAS 190
           AALT++DTC++GF   +  + +L+R  V++V  +TSNALAL+N  A++
Sbjct: 260 AALTNDDTCMDGFSLVKTAVKDLVRRHVVEVARLTSNALALINMYAST 307

[Close]

BLAST of Bhi09G000717 vs. ExPASy Swiss-Prot
Match: Q84WE4 (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 9.0e-22
Identity = 56/167 (33.53%), Postives = 100/167 (59.88%), Query Frame = 0


Query: 26  HSTKTSYVREACSVTRHQDLCIQSLTPFSSTAKRSPAKWARAGVSVTITEAKTVAGLLGR 85
           H+     V  +C    +  LC+++L+ +S     +    A+A + ++++ A++ A  L  
Sbjct: 27  HNGAEDIVHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQAAIKISLSHAQSAAKKLAV 86

Query: 86  LKNNKRMKGRNRAAVSDCVEVFEAAIDELHRSLGVLRRL-----SRSNFDAQIGDLTTWV 145
           ++++   K + +AA+ DCVE+   ++DEL R+LGVL+ L     S   F  Q+ +  TW 
Sbjct: 87  VRDSVGKKKQEKAALVDCVEMIGDSVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQTWA 146

Query: 146 SAALTDEDTCVEGFEG-ERGKLVNLIRNRVLKVGYITSNALALVNKL 187
           SAALTD+DTC++GF+G + G++   ++  + KV  +TSNAL +VN+L
Sbjct: 147 SAALTDDDTCLDGFQGMDDGEIKTEVKQWMTKVARVTSNALYMVNQL 193

[Close]

BLAST of Bhi09G000717 vs. ExPASy TrEMBL
Match: A0A5D3D1R1 (21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00630 PE=4 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 8.3e-87
Identity = 170/196 (86.73%), Postives = 181/196 (92.35%), Query Frame = 0


Query: 1   MLRAPNTRASIATFLALISILPRLTHSTKTSYVREACSVTRHQDLCIQSLTPFSSTAKRS 60
           M+R P+TRASIA  LALISILP LTHS KTSYV+EAC VT HQDLCIQSL+PFSSTAKRS
Sbjct: 1   MMRPPSTRASIAALLALISILPWLTHSAKTSYVQEACRVTLHQDLCIQSLSPFSSTAKRS 60

Query: 61  PAKWARAGVSVTITEAKTVAGLLGRLKNNKRMKGRNRAAVSDCVEVFEAAIDELHRSLGV 120
           P KWARAGVSVTITEAK VA LLGRLKNNKRMKGRNRAAV DCVEVF+AAIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAALLGRLKNNKRMKGRNRAAVLDCVEVFDAAIDELHRSLGV 120

Query: 121 LRRLSRSNFDAQIGDLTTWVSAALTDEDTCVEGFEGERGKLVNLIRNRVLKVGYITSNAL 180
           LRRLSR NFD+Q+GDLTTWVSAALTDEDTCVEGFEGERGK+V+L+RNRV+KVGYITSNAL
Sbjct: 121 LRRLSRRNFDSQMGDLTTWVSAALTDEDTCVEGFEGERGKVVSLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASGLETAPN 197
           ALVNKLA SG ETA N
Sbjct: 181 ALVNKLATSGFETALN 196

[Close]

BLAST of Bhi09G000717 vs. ExPASy TrEMBL
Match: A0A1S3BSW5 (21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492810 PE=4 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 8.3e-87
Identity = 170/196 (86.73%), Postives = 181/196 (92.35%), Query Frame = 0


Query: 1   MLRAPNTRASIATFLALISILPRLTHSTKTSYVREACSVTRHQDLCIQSLTPFSSTAKRS 60
           M+R P+TRASIA  LALISILP LTHS KTSYV+EAC VT HQDLCIQSL+PFSSTAKRS
Sbjct: 1   MMRPPSTRASIAALLALISILPWLTHSAKTSYVQEACRVTLHQDLCIQSLSPFSSTAKRS 60

Query: 61  PAKWARAGVSVTITEAKTVAGLLGRLKNNKRMKGRNRAAVSDCVEVFEAAIDELHRSLGV 120
           P KWARAGVSVTITEAK VA LLGRLKNNKRMKGRNRAAV DCVEVF+AAIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAALLGRLKNNKRMKGRNRAAVLDCVEVFDAAIDELHRSLGV 120

Query: 121 LRRLSRSNFDAQIGDLTTWVSAALTDEDTCVEGFEGERGKLVNLIRNRVLKVGYITSNAL 180
           LRRLSR NFD+Q+GDLTTWVSAALTDEDTCVEGFEGERGK+V+L+RNRV+KVGYITSNAL
Sbjct: 121 LRRLSRRNFDSQMGDLTTWVSAALTDEDTCVEGFEGERGKVVSLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASGLETAPN 197
           ALVNKLA SG ETA N
Sbjct: 181 ALVNKLATSGFETALN 196

[Close]

BLAST of Bhi09G000717 vs. ExPASy TrEMBL
Match: A0A0A0KAX0 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G397050 PE=4 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.4e-86
Identity = 171/196 (87.24%), Postives = 178/196 (90.82%), Query Frame = 0


Query: 1   MLRAPNTRASIATFLALISILPRLTHSTKTSYVREACSVTRHQDLCIQSLTPFSSTAKRS 60
           MLRAP+ RASI   LALISILP LTHS KTSYV+EAC VTRHQDLCIQSL+PFSS AKRS
Sbjct: 1   MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRS 60

Query: 61  PAKWARAGVSVTITEAKTVAGLLGRLKNNKRMKGRNRAAVSDCVEVFEAAIDELHRSLGV 120
           P KWARAGVSVTITEAK VAGLLGRLKNNKRMKGRNRAAV DCVEVFEAAIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGV 120

Query: 121 LRRLSRSNFDAQIGDLTTWVSAALTDEDTCVEGFEGERGKLVNLIRNRVLKVGYITSNAL 180
           LRRLSR NFDAQ+GDLTTWVSAALTDEDTCVEGFEGE GK+V L+RNRV+KVGYITSNAL
Sbjct: 121 LRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASGLETAPN 197
           ALVNKLAAS  ET  N
Sbjct: 181 ALVNKLAASSFETTIN 196

[Close]

BLAST of Bhi09G000717 vs. ExPASy TrEMBL
Match: A0A6J1L076 (pectinesterase inhibitor 6 OS=Cucurbita maxima OX=3661 GN=LOC111497889 PE=4 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.9e-83
Identity = 165/196 (84.18%), Postives = 176/196 (89.80%), Query Frame = 0


Query: 1   MLRAPNTRASIATFLALISILPRLTHSTKTSYVREACSVTRHQDLCIQSLTPFSSTAKRS 60
           MLRAP +R SI T LA I+ILP LTHS +TSYV+EACSVT H DLCI+SL+PFSSTAKRS
Sbjct: 1   MLRAPTSRTSIVTLLAFIAILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60

Query: 61  PAKWARAGVSVTITEAKTVAGLLGRLKNNKRMKGRNRAAVSDCVEVFEAAIDELHRSLGV 120
           P KWARA VSVTITEAK VA  L RLKNN+RMKGRNRAAVSDCVEVFE AIDELHRSLGV
Sbjct: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120

Query: 121 LRRLSRSNFDAQIGDLTTWVSAALTDEDTCVEGFEGERGKLVNLIRNRVLKVGYITSNAL 180
           LRRLSRS FDAQ+GDLTTWVSAALTDEDTCV+GFEGERG+LVNLIRNRV+K  YITSNAL
Sbjct: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180

Query: 181 ALVNKLAASGLETAPN 197
           ALVNKLAASG ETAPN
Sbjct: 181 ALVNKLAASGFETAPN 196

[Close]

BLAST of Bhi09G000717 vs. ExPASy TrEMBL
Match: A0A6J1H9V5 (pectinesterase inhibitor 6-like OS=Cucurbita moschata OX=3662 GN=LOC111460929 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 7.2e-83
Identity = 164/196 (83.67%), Postives = 174/196 (88.78%), Query Frame = 0


Query: 1   MLRAPNTRASIATFLALISILPRLTHSTKTSYVREACSVTRHQDLCIQSLTPFSSTAKRS 60
           MLRAP +R SI T LA ISILP LTHS +TSYV+EACSVT H DLCI+SL+PFSSTAKRS
Sbjct: 63  MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 122

Query: 61  PAKWARAGVSVTITEAKTVAGLLGRLKNNKRMKGRNRAAVSDCVEVFEAAIDELHRSLGV 120
           P KWARA VSVTITEAK VA  L RLKNN+RMKGRNRAAVSDCVEVFE AIDELHRSLGV
Sbjct: 123 PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 182

Query: 121 LRRLSRSNFDAQIGDLTTWVSAALTDEDTCVEGFEGERGKLVNLIRNRVLKVGYITSNAL 180
           LRRLSRS FDAQ+GDLTTWVSAALTDEDTCV+GFEGERG+LVNLIRNR +K  YITSNAL
Sbjct: 183 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRAVKATYITSNAL 242

Query: 181 ALVNKLAASGLETAPN 197
           ALVNKLAASG ET PN
Sbjct: 243 ALVNKLAASGFETGPN 258

The following BLAST results are available for this feature:

BLAST of Bhi09G000717 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62820.1	3.4e-40	49.46	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT2G47670.1	3.8e-36	48.48	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62360.1	2.6e-32	39.36	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G12390.1	3.6e-26	37.28	pectin methylesterase inhibitor 1	[more]
AT2G01610.1	3.4e-24	34.69	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of Bhi09G000717 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22244	5.4e-35	48.48	Pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PMEI6 PE=2 SV=1	[more]
P17407	1.7e-25	35.33	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SB37	1.1e-22	36.31	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9SI74	2.4e-22	31.55	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
Q84WE4	9.0e-22	33.53	Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1	[more]

BLAST of Bhi09G000717 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D1R1	8.3e-87	86.73	21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00630...	[more]
A0A1S3BSW5	8.3e-87	86.73	21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492810 PE=4 SV=1	[more]
A0A0A0KAX0	1.4e-86	87.24	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G397050 PE=4 S...	[more]
A0A6J1L076	1.9e-83	84.18	pectinesterase inhibitor 6 OS=Cucurbita maxima OX=3661 GN=LOC111497889 PE=4 SV=1	[more]
A0A6J1H9V5	7.2e-83	83.67	pectinesterase inhibitor 6-like OS=Cucurbita moschata OX=3662 GN=LOC111460929 PE...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 28..182 e-value: 5.0E-47 score: 172.2
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 32..182 e-value: 5.2E-32 score: 111.3
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 9..186 e-value: 6.9E-36 score: 121.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 27..188 e-value: 2.3E-34 score: 120.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 31..186
None	No IPR available	PANTHER	PTHR31080:SF158	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 19..181
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 19..181
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 36..188 e-value: 7.63084E-45 score: 144.118

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M000717	Bhi09M000717	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity