Bhi09G000526 (gene) Wax gourd (B227) v1

Overview


Name	Bhi09G000526
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	E3 ubiquitin ligase BIG BROTHER
Location	chr9: 13592173 .. 13596029 (+)
RNA-Seq Expression	Bhi09G000526
Synteny	Bhi09G000526

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAAACTTCAAAATACCCATTTGCCATTTGAAGAAGATTTCAAAGCCCATCAACTAGCTTAAAAACCTTCACTTGCACAGAGATGGAAAAAATGGATTATAGCCAGAAAACAAATTGAAGAACAGAGAAAGAAAGGGAACACAAAACCATACCCCCATTTCCTTTCATTTTCATCTCTCTCTGCTAAAACTCTGCCAGTAATCAAGAGTTTTGGGGAGAGAGAATCAGAAGAAAAAAAAAAAAGGAGTTTTAGAGAGAGAGTTTAAGGTCCATCCTCCATTTCTCAAGCTTCCATTCTACTTCATTTCTCAAGCTCTTATTGTTTGTTTGTTTGTTAAGTTTTAGGAGAAGAAGCCTTTTTTCTGTTTTCTACATTTTTTGTTCTTTTTTTGCTGTGTTTTTTTTTTTTTGAATATTATGAAGATTCTAATGAATACTGGGATCTGTACCTGTTTATTTTTTTGTTCATTTGTCTGCATTTATCTGATTCTTAGGATTCTCTAACATCTTTTATACTCGTCTGTCTACATTTGTCTAATTCCTCAGATTTTTCCTCTGTCAGAGTGTGAAGAGGTTTCTTGGGGGAGTTTCTGTTTGGCTATCCAGAAAGAAAAATCATAGGATTTTGCCTTGTTTTTTCATTTTTGGAGACTAAAGAAGCTAAATGTAAGTTTGTCATTTGGTTTTGTGTAATGTTAATGTTTGTTTTTTGTTTTTGTTTTTAAGTCTTCGAGTGTTTTAGGGCCTGTTTCCTATTTCATTAGAATGAAAATTTCAAATCTGCAATTTGCTTAGCTTTCTCACCTTTTCCCCTAAGTTTTCTTAGCAACCAAACAGATTGTTCTGGATGAGTTCATTCTATGGAAGGATAATCACATTTTGCTTGAAATTTCCAAAGCCTTGGCAATGTAGGTTTTAAGTTCATTTCATTTTTCCTTTTGTTTGATATGTTCTTTCTTTTGGTATTTTTGCAGTTAGTTTTGCAAATTCTACTGTTGACTGCTGCTTAAGATGAATGAAGATAGACAAATGGAAGTACATTATATCGACACCGGGTTTCCTTACACAGTTACGGAGAGCTTCATGGATTTCTTTGAAGGGCTTCACCAACATCAAGTACCTGCTTATGGCAATGCTGTACCATTGCTCGATCAGGTAATGAAGCGGCCGTGAAAAGCTGATATAATTTGTTTGTCAGTTGAGAAAATAGTAATATTGGAAACGTATGATAATTTGTTTCTCAGGGAAATGCTTACTGGTCAATGAATATGCAATGTTACAAATTTGGAGTATCTGATCATGGGAATACTTATTATGGCCATTCGGAGGAAAGTCACCATTTACCACCTTCAATGGATGTTGGTGAAAGGGAATGGGAGTATCCTACTTCAGTGGCGAATGTCGAAATTCCCGAACCAACATATGCTCCATCAGTGGAGGAGGATGTTGTAGATGCTCATTCTATACCAGAAGAATGTAAGTGTGACAAATCTGATTGTGACAAATCTGATTCTGAGTCTGTTTATTCCATACTTTTGAATACTATCATCGAGCTTGTTGTTATCACATTGATGAGTTGAATTGTTGAAAATGTTTGAGAAACACAAAAATAGTGGATATTATCAGCATTTTTTTACTTGTTTTCCTGTTTTGTAGGTGATTTAAGCCATCAGGAGGAGACCAGTACTTCTCAAGTATGTCTCCTTGATTCCACAATATGCGTCCTTTTCTGTTATGACCATCATCTACTTAATACCTTTTATGAACTGTGGCTTAATCGAAAAGTTATTCGGGACTGTTTTAAGTTATTCGTTAAATAATGAATTATAGTACAAATCTTATTATCGTCAAAGGTTGTAGAATGTTCAGATGTTATCATTCTGAAGTTATATGGGTGATGAATTTCTTTTCACCTCAAGTTTTAGTTTTTCATGTCAAAATGTTTCTCTTTATGATCAATTTATTGCCCGACCAAATTTCGTTGCTTTTGCTCTATGAGAATAGGTACTACTTTGGTTTAGGGCTCATTGAATCCATCTTCTTTTGTTCGTCTCTTCAGGTTACATGGCAAGATGAGATTGATCCTGACCACATGACCTATGAGGTATGCTTTCAACTCTTTCATTTAGAAGCCAGATTTGTTTAAGAAGTTGTTTTAAGAAATGTTCTGTAGATCTGCAATTGATTGCCCTATAGAAGTTGAGGTTGTTGTACACTTGAAACCGATGATTTACCAAGCACGTGATTATTTTTGCTTTAAAGAGAAGGTTTTAGCTCAATTATCAACAAGCCCCTAGTTTCTTATGGCCATATTATCTATGGTTGGTTGTCAAATAAATGGGATTCATAGAAGTTTACTGTGTCAAAAGAAGAGACAAAGGGACTGTCTTTAGCCATGACTGTGTGGATCGTCTTTGACAGGAACTGTTGGACTTAGGTGAATCAGTTGGAACTGAAAGTCGGGGTCTTTCTGAAGAACAAATAAGTTTGCTTCCGACTGCGCGATTCAAATTAACGAGCTTTTTCTCGAGAAAAAAGCCCGAGGAGAGGTACAATTTTTACTTAAGTTGCATTTGAGTTATCTTGCACTATGTTAGTGCACTTGGTGGATATATAAGGACATTCCTGTCAATTTAGACAACAGTTTCTACGAATCGAGATGTACGACATTGTCATAACACAATTGATGCTTGTTTCTGATGTGCCTACTCTAAAGTCATATGGATCCATATTTCTGTCATAGTACAGGAGTTAATACAGTCTAATGAATCCATTCACCTTGGTTTCTTCTGTTTTTAAGTGTAGCAATGTCGAACTTGAACCTCTTAACAAATCTTACAAATAAGACAGTTATATGAGATGCACTCTTTTGGGTATGATCATGCTATTGAAGTTGCATCTCAAGTTGCTGACTATAGGTCTTGGTACAAGTAGGTGTTAACATCTCGGTACCTTCCCGGTACGGTGTTTATCCTTGGAATCTTTCTTCAATATTTGATTGTACTTGCTTTCTTTGGACCGCAGATGTGTGATTTGCCAAATGAGGTACAAGAGAGGGGACAAACAAATAAAATTACCCTGCAAGCATTTTTACCACAACAAATGCATAACCAAATGGCTGACCATCAACAAAGTTAGTTGTTTTCTTGGCTTTCTCATGTCTTTATACTTCCACAATTTCTGATCTAACTTGATATTTTATCTCGCCACATCATCTTCGTGTTTATCAGATATGTCCGATCTGCAACATCGAGGTGTTCGGAGATGGAAGCATGAATGCATCAGGAAACGATAACCGACAGTAGATTTTGGAAGCTCTAACATTTTGTTCCTCATTCATCTTTTCCCATTTTTGCCTTCTCACTTTCATTTCTTTCCCCCATTTTGTACATCTCTACTTAACTAAGTGAATTAAGTTCTCTAATCCTTAATCTCTCTCTCCCCACATTTGCCCTATGCTCACAGAAAGAAAAGTGTACCCCTCTTTTAACTTGTCAACTGCGTAGCCTGAAGATTGAAGTCTAGTTATTTTCTTAGATTAAGATTAGCTTATTTTGTAGTCATTTTTCGGATTGAGGGTCGTGTCAAACTGCATTGAATGAGGATGTTCGTAGGTTGGGGTGAGTTAGATTAGGTTAATATAGACGTCTTGGATCTAATCTAATTGTTCAAGTTACATATTTGTTCAATCTAAGTGACTTTTGTTAGTAATAGACCATGAATTCATTTGGTCATTTATTTCAAGTTTTGTAAAAGAAATAGTGAGTGATAATTTTTTAATTTTAATAATTAATTTATTTTCTGTATTTTATTTCATTTTATCTGGAAATTATTAGAGAATAAAATTAAGCTAGAAG

mRNA sequence

AGAAACTTCAAAATACCCATTTGCCATTTGAAGAAGATTTCAAAGCCCATCAACTAGCTTAAAAACCTTCACTTGCACAGAGATGGAAAAAATGGATTATAGCCAGAAAACAAATTGAAGAACAGAGAAAGAAAGGGAACACAAAACCATACCCCCATTTCCTTTCATTTTCATCTCTCTCTGCTAAAACTCTGCCAGTAATCAAGAGTTTTGGGGAGAGAGAATCAGAAGAAAAAAAAAAAAGGAGTTTTAGAGAGAGAGTTTAAGATTTTTCCTCTGTCAGAGTGTGAAGAGGTTTCTTGGGGGAGTTTCTGTTTGGCTATCCAGAAAGAAAAATCATAGGATTTTGCCTTGTTTTTTCATTTTTGGAGACTAAAGAAGCTAAATTTAGTTTTGCAAATTCTACTGTTGACTGCTGCTTAAGATGAATGAAGATAGACAAATGGAAGTACATTATATCGACACCGGGTTTCCTTACACAGTTACGGAGAGCTTCATGGATTTCTTTGAAGGGCTTCACCAACATCAAGTACCTGCTTATGGCAATGCTGTACCATTGCTCGATCAGGGAAATGCTTACTGGTCAATGAATATGCAATGTTACAAATTTGGAGTATCTGATCATGGGAATACTTATTATGGCCATTCGGAGGAAAGTCACCATTTACCACCTTCAATGGATGTTGGTGAAAGGGAATGGGAGTATCCTACTTCAGTGGCGAATGTCGAAATTCCCGAACCAACATATGCTCCATCAGTGGAGGAGGATGTTGTAGATGCTCATTCTATACCAGAAGAATGTGATTTAAGCCATCAGGAGGAGACCAGTACTTCTCAAGTTACATGGCAAGATGAGATTGATCCTGACCACATGACCTATGAGGAACTGTTGGACTTAGGTGAATCAGTTGGAACTGAAAGTCGGGGTCTTTCTGAAGAACAAATAAGTTTGCTTCCGACTGCGCGATTCAAATTAACGAGCTTTTTCTCGAGAAAAAAGCCCGAGGAGAGATGTGTGATTTGCCAAATGAGGTACAAGAGAGGGGACAAACAAATAAAATTACCCTGCAAGCATTTTTACCACAACAAATGCATAACCAAATGGCTGACCATCAACAAAATATGTCCGATCTGCAACATCGAGGTGTTCGGAGATGGAAGCATGAATGCATCAGGAAACGATAACCGACAGTAGATTTTGGAAGCTCTAACATTTTGTTCCTCATTCATCTTTTCCCATTTTTGCCTTCTCACTTTCATTTCTTTCCCCCATTTTGTACATCTCTACTTAACTAAGTGAATTAAGTTCTCTAATCCTTAATCTCTCTCTCCCCACATTTGCCCTATGCTCACAGAAAGAAAAGTGTACCCCTCTTTTAACTTGTCAACTGCGTAGCCTGAAGATTGAAGTCTAGTTATTTTCTTAGATTAAGATTAGCTTATTTTGTAGTCATTTTTCGGATTGAGGGTCGTGTCAAACTGCATTGAATGAGGATGTTCGTAGGTTGGGGTGAGTTAGATTAGGTTAATATAGACGTCTTGGATCTAATCTAATTGTTCAAGTTACATATTTGTTCAATCTAAGTGACTTTTGTTAGTAATAGACCATGAATTCATTTGGTCATTTATTTCAAGTTTTGTAAAAGAAATAGTGAGTGATAATTTTTTAATTTTAATAATTAATTTATTTTCTGTATTTTATTTCATTTTATCTGGAAATTATTAGAGAATAAAATTAAGCTAGAAG

Coding sequence (CDS)

ATGAATGAAGATAGACAAATGGAAGTACATTATATCGACACCGGGTTTCCTTACACAGTTACGGAGAGCTTCATGGATTTCTTTGAAGGGCTTCACCAACATCAAGTACCTGCTTATGGCAATGCTGTACCATTGCTCGATCAGGGAAATGCTTACTGGTCAATGAATATGCAATGTTACAAATTTGGAGTATCTGATCATGGGAATACTTATTATGGCCATTCGGAGGAAAGTCACCATTTACCACCTTCAATGGATGTTGGTGAAAGGGAATGGGAGTATCCTACTTCAGTGGCGAATGTCGAAATTCCCGAACCAACATATGCTCCATCAGTGGAGGAGGATGTTGTAGATGCTCATTCTATACCAGAAGAATGTGATTTAAGCCATCAGGAGGAGACCAGTACTTCTCAAGTTACATGGCAAGATGAGATTGATCCTGACCACATGACCTATGAGGAACTGTTGGACTTAGGTGAATCAGTTGGAACTGAAAGTCGGGGTCTTTCTGAAGAACAAATAAGTTTGCTTCCGACTGCGCGATTCAAATTAACGAGCTTTTTCTCGAGAAAAAAGCCCGAGGAGAGATGTGTGATTTGCCAAATGAGGTACAAGAGAGGGGACAAACAAATAAAATTACCCTGCAAGCATTTTTACCACAACAAATGCATAACCAAATGGCTGACCATCAACAAAATATGTCCGATCTGCAACATCGAGGTGTTCGGAGATGGAAGCATGAATGCATCAGGAAACGATAACCGACAGTAG

Protein sequence

MNEDRQMEVHYIDTGFPYTVTESFMDFFEGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSVANVEIPEPTYAPSVEEDVVDAHSIPEECDLSHQEETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARFKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGSMNASGNDNRQ

Homology

[Close]

BLAST of Bhi09G000526 vs. TAIR 10
Match: AT3G63530.1 (RING/U-box superfamily protein )

HSP 1 Score: 250.4 bits (638), Expect = 1.6e-66
Identity = 125/251 (49.80%), Postives = 171/251 (68.13%), Query Frame = 0


Query: 1   MNEDRQ--MEVHYIDTGFPYTVTESFMDFFEGLHQHQVPAYGNAVPLLDQGNAYWSMNMQ 60
           MN D +   + HY +TGFPY  T S+MDF+ G  Q  +  Y +A  +  Q N YW+MN  
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPL-NYDHAATMHPQDNLYWTMNTN 60

Query: 61  CYKFGVSDHGN-TYYGHSEESHHLPPSMDVGEREWEYPTSVANVEIPEPTYAPSVEEDVV 120
            YKFG S   N ++YG  + + HL   M +G   W+Y   V   + PE T A SV+    
Sbjct: 61  AYKFGFSGSDNASFYGSYDMNDHL-SRMSIGRTNWDYHPMVNVADDPENTVARSVQIGDT 120

Query: 121 DAHSIPEECDLSHQEETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLL 180
           D HS  EEC ++++ +  + QV+WQD+IDPD MTYEEL++LGE+VGTESRGLS+E I  L
Sbjct: 121 DEHSEAEEC-IANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETL 180

Query: 181 PTARFKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPIC 240
           PT ++K  S FSRK+  ERCVICQ++YK G++Q+ LPCKH YH++CI+KWL+INK+CP+C
Sbjct: 181 PTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVC 240

Query: 241 NIEVFGDGSMN 249
           N EVFG+ S++
Sbjct: 241 NSEVFGEPSIH 248

[Close]

BLAST of Bhi09G000526 vs. TAIR 10
Match: AT3G63530.2 (RING/U-box superfamily protein )

HSP 1 Score: 250.4 bits (638), Expect = 1.6e-66
Identity = 125/251 (49.80%), Postives = 171/251 (68.13%), Query Frame = 0


Query: 1   MNEDRQ--MEVHYIDTGFPYTVTESFMDFFEGLHQHQVPAYGNAVPLLDQGNAYWSMNMQ 60
           MN D +   + HY +TGFPY  T S+MDF+ G  Q  +  Y +A  +  Q N YW+MN  
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPL-NYDHAATMHPQDNLYWTMNTN 60

Query: 61  CYKFGVSDHGN-TYYGHSEESHHLPPSMDVGEREWEYPTSVANVEIPEPTYAPSVEEDVV 120
            YKFG S   N ++YG  + + HL   M +G   W+Y   V   + PE T A SV+    
Sbjct: 61  AYKFGFSGSDNASFYGSYDMNDHL-SRMSIGRTNWDYHPMVNVADDPENTVARSVQIGDT 120

Query: 121 DAHSIPEECDLSHQEETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLL 180
           D HS  EEC ++++ +  + QV+WQD+IDPD MTYEEL++LGE+VGTESRGLS+E I  L
Sbjct: 121 DEHSEAEEC-IANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETL 180

Query: 181 PTARFKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPIC 240
           PT ++K  S FSRK+  ERCVICQ++YK G++Q+ LPCKH YH++CI+KWL+INK+CP+C
Sbjct: 181 PTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVC 240

Query: 241 NIEVFGDGSMN 249
           N EVFG+ S++
Sbjct: 241 NSEVFGEPSIH 248

[Close]

BLAST of Bhi09G000526 vs. TAIR 10
Match: AT3G19910.1 (RING/U-box superfamily protein )

HSP 1 Score: 119.8 bits (299), Expect = 3.2e-27
Identity = 57/111 (51.35%), Postives = 79/111 (71.17%), Query Frame = 0


Query: 131 QEETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARFKLTSFFSR 190
           ++E+ TSQ  W DE+DPD ++YEELL LG+ VGTESRGLS + I+ LP+ R+K     ++
Sbjct: 225 EDESHTSQDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEGD--NQ 284

Query: 191 KKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEV 242
               E CVIC++ Y+  +  I LPCKH YH++CI  WL INK+CP+C+ EV
Sbjct: 285 NGTNESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEV 332

[Close]

BLAST of Bhi09G000526 vs. TAIR 10
Match: AT5G52140.1 (RING/U-box superfamily protein )

HSP 1 Score: 99.8 bits (247), Expect = 3.5e-21
Identity = 63/180 (35.00%), Postives = 94/180 (52.22%), Query Frame = 0


Query: 74  HSEESHHLPPSMDVGEREWEYPTSVANVEIPEPTYAPSVEEDVV---DAHSIPEECDLSH 133
           H E +++    +        +P  V    IP+   APS    +    D  +       +H
Sbjct: 101 HDELANNTEDDIQETHHHQYHPMQVNRSYIPQRD-APSSSRAIFHYDDGENFSGRYTHTH 160

Query: 134 QEETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARF--KLTSFF 193
              + +   T  +  DP++MTYEEL +LG+SVG   +GLS+E+IS L T ++  K  S++
Sbjct: 161 SPSSISHNPTDDENNDPENMTYEELTELGDSVGDVGKGLSQERISRLRTHKYGTKTKSWY 220

Query: 194 ---SRKK---PEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVF 243
               +KK    + +C IC M Y +GDK   LPCKH YH  CI++WL  NK+C IC  EV+
Sbjct: 221 CLHMKKKFVADDSQCSICLMEYAKGDKITTLPCKHIYHKDCISQWLKQNKVCCICKAEVY 279

[Close]

BLAST of Bhi09G000526 vs. TAIR 10
Match: AT3G47180.1 (RING/U-box superfamily protein )

HSP 1 Score: 97.8 bits (242), Expect = 1.3e-20
Identity = 55/128 (42.97%), Postives = 80/128 (62.50%), Query Frame = 0


Query: 112 VEEDVVDAHSIPEECDLSHQEETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSE 171
           V+ED ++     +E + + +EE    +   +DEIDPD ++YEEL+ LG+ +G E+RGL+ 
Sbjct: 81  VDEDEINEFLEDQESNSNLEEEDDFLEE--EDEIDPDQLSYEELIALGDFIGVENRGLTP 140

Query: 172 EQISLLPTARFKLTSFFSRKKPE-ERCVICQMRYKRGDKQIKL-PCKHFYHNKCITKWLT 231
            +IS    A    T  FS  K E +RCV+CQM ++  +  + L PC H YH++CITKWL 
Sbjct: 141 IEISTCLNAS---TYVFSHNKNEIDRCVVCQMEFEERESLVVLRPCDHPYHSECITKWLE 200

Query: 232 INKICPIC 238
             KICPIC
Sbjct: 201 TKKICPIC 203

[Close]

BLAST of Bhi09G000526 vs. ExPASy Swiss-Prot
Match: Q8L649 (E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE=1 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 2.2e-65
Identity = 125/251 (49.80%), Postives = 171/251 (68.13%), Query Frame = 0


Query: 1   MNEDRQ--MEVHYIDTGFPYTVTESFMDFFEGLHQHQVPAYGNAVPLLDQGNAYWSMNMQ 60
           MN D +   + HY +TGFPY  T S+MDF+ G  Q  +  Y +A  +  Q N YW+MN  
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPL-NYDHAATMHPQDNLYWTMNTN 60

Query: 61  CYKFGVSDHGN-TYYGHSEESHHLPPSMDVGEREWEYPTSVANVEIPEPTYAPSVEEDVV 120
            YKFG S   N ++YG  + + HL   M +G   W+Y   V   + PE T A SV+    
Sbjct: 61  AYKFGFSGSDNASFYGSYDMNDHL-SRMSIGRTNWDYHPMVNVADDPENTVARSVQIGDT 120

Query: 121 DAHSIPEECDLSHQEETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLL 180
           D HS  EEC ++++ +  + QV+WQD+IDPD MTYEEL++LGE+VGTESRGLS+E I  L
Sbjct: 121 DEHSEAEEC-IANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETL 180

Query: 181 PTARFKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPIC 240
           PT ++K  S FSRK+  ERCVICQ++YK G++Q+ LPCKH YH++CI+KWL+INK+CP+C
Sbjct: 181 PTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVC 240

Query: 241 NIEVFGDGSMN 249
           N EVFG+ S++
Sbjct: 241 NSEVFGEPSIH 248

[Close]

BLAST of Bhi09G000526 vs. ExPASy Swiss-Prot
Match: Q9LT17 (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 4.6e-26
Identity = 57/111 (51.35%), Postives = 79/111 (71.17%), Query Frame = 0


Query: 131 QEETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARFKLTSFFSR 190
           ++E+ TSQ  W DE+DPD ++YEELL LG+ VGTESRGLS + I+ LP+ R+K     ++
Sbjct: 225 EDESHTSQDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEGD--NQ 284

Query: 191 KKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEV 242
               E CVIC++ Y+  +  I LPCKH YH++CI  WL INK+CP+C+ EV
Sbjct: 285 NGTNESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEV 332

[Close]

BLAST of Bhi09G000526 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 6.8e-14
Identity = 38/93 (40.86%), Postives = 51/93 (54.84%), Query Frame = 0


Query: 145 IDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARFKLTSFFSRKKPEERCVICQMRY 204
           +D D+M+YEELL LGE +G  S GLSEE I  +       +S     +  E C +CQ  Y
Sbjct: 567 LDVDNMSYEELLALGERIGDVSTGLSEEVILKVMKQHKHTSSAAGSHQDMEPCCVCQEEY 626

Query: 205 KRGDKQIKLPCKHFYHNKCITKWLTINKICPIC 238
             GD    L C H +H  C+ +WL +  +CPIC
Sbjct: 627 AEGDDLGTLGCGHEFHTACVKQWLMLKNLCPIC 659

[Close]

BLAST of Bhi09G000526 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 2.6e-13
Identity = 39/93 (41.94%), Postives = 51/93 (54.84%), Query Frame = 0


Query: 145 IDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARFKLTSFFSRKKPEERCVICQMRY 204
           +D D+M+YEELL L E +G  S GLSEE+++ L   R   +        EE C ICQ  Y
Sbjct: 568 LDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRLEASVEEEPCCICQEEY 627

Query: 205 KRGDKQIKLPCKHFYHNKCITKWLTINKICPIC 238
             GD    L C H +H  C+ +WL +   CPIC
Sbjct: 628 VDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPIC 660

[Close]

BLAST of Bhi09G000526 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.6e-13
Identity = 44/100 (44.00%), Postives = 54/100 (54.00%), Query Frame = 0


Query: 145 IDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARFKLT----SFFSRKKPEERCVIC 204
           +D D+M+YEELL LGE +G  S GLSEE I L    + K T    S     +  E C IC
Sbjct: 601 LDVDNMSYEELLALGERIGDVSTGLSEEVI-LKAMKQHKHTSSSPSSVELHQNIEPCCIC 660

Query: 205 QMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 241
           Q  Y  GD    L C H +H  CI +W+ I  +CPIC  E
Sbjct: 661 QEEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

[Close]

BLAST of Bhi09G000526 vs. ExPASy TrEMBL
Match: A0A1S3CED1 (E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499919 PE=4 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 4.4e-141
Identity = 240/254 (94.49%), Postives = 247/254 (97.24%), Query Frame = 0


Query: 1   MNEDRQMEVHYIDTGFPYTVTESFMDFFEGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60
           MNEDRQME+HY+DTGFPYT TESFMDFF+GLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSVANVEIPEPTYAPSVEEDVVDAH 120
           KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTS  NVEIPEP+YAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEECDLSHQEETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180
           SIPEECDLSH+EETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA
Sbjct: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180

Query: 181 RFKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 240
           R KLTSFFSRKK +ERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE
Sbjct: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 240

Query: 241 VFGDGSMNASGNDN 255
           VFGDGS NAS +DN
Sbjct: 241 VFGDGSTNASASDN 254

[Close]

BLAST of Bhi09G000526 vs. ExPASy TrEMBL
Match: A0A5D3CGQ8 (E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00890 PE=4 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 2.2e-140
Identity = 239/254 (94.09%), Postives = 246/254 (96.85%), Query Frame = 0


Query: 1   MNEDRQMEVHYIDTGFPYTVTESFMDFFEGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60
           MNEDRQME+HY+DTGFPYT TESFMDFF+GLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSVANVEIPEPTYAPSVEEDVVDAH 120
           KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTS  NVEIPEP+YAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEECDLSHQEETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180
           SIPEECDLSH+EETSTSQV WQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA
Sbjct: 121 SIPEECDLSHREETSTSQVIWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180

Query: 181 RFKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 240
           R KLTSFFSRKK +ERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE
Sbjct: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 240

Query: 241 VFGDGSMNASGNDN 255
           VFGDGS NAS +DN
Sbjct: 241 VFGDGSTNASASDN 254

[Close]

BLAST of Bhi09G000526 vs. ExPASy TrEMBL
Match: A0A1S3CES7 (E3 ubiquitin ligase BIG BROTHER isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499919 PE=4 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.3e-137
Identity = 234/248 (94.35%), Postives = 241/248 (97.18%), Query Frame = 0


Query: 7   MEVHYIDTGFPYTVTESFMDFFEGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD 66
           ME+HY+DTGFPYT TESFMDFF+GLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD
Sbjct: 1   MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD 60

Query: 67  HGNTYYGHSEESHHLPPSMDVGEREWEYPTSVANVEIPEPTYAPSVEEDVVDAHSIPEEC 126
           HGNTYYGHSEESHHLPPSMDVGEREWEYPTS  NVEIPEP+YAPSVEEDVVDAHSIPEEC
Sbjct: 61  HGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAHSIPEEC 120

Query: 127 DLSHQEETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARFKLTS 186
           DLSH+EETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTAR KLTS
Sbjct: 121 DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS 180

Query: 187 FFSRKKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGS 246
           FFSRKK +ERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGS
Sbjct: 181 FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGS 240

Query: 247 MNASGNDN 255
            NAS +DN
Sbjct: 241 TNASASDN 248

[Close]

BLAST of Bhi09G000526 vs. ExPASy TrEMBL
Match: A0A0A0K8C4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428220 PE=4 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 3.0e-137
Identity = 234/248 (94.35%), Postives = 241/248 (97.18%), Query Frame = 0


Query: 7   MEVHYIDTGFPYTVTESFMDFFEGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD 66
           ME+HY+DTGFPYT TESFMDFF+GLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD
Sbjct: 1   MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD 60

Query: 67  HGNTYYGHSEESHHLPPSMDVGEREWEYPTSVANVEIPEPTYAPSVEEDVVDAHSIPEEC 126
           HGNTYYGHSEESHHLPPSMDVGEREWEYPTS  NVEIPEP+YAPSVEEDVVDAHSIPEEC
Sbjct: 61  HGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAHSIPEEC 120

Query: 127 DLSHQEETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARFKLTS 186
           DLSH+EETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTAR KLTS
Sbjct: 121 DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS 180

Query: 187 FFSRKKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGS 246
           FFSRKK +ERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGS
Sbjct: 181 FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGS 240

Query: 247 MNASGNDN 255
            NAS +DN
Sbjct: 241 TNASASDN 248

[Close]

BLAST of Bhi09G000526 vs. ExPASy TrEMBL
Match: A0A6J1D3R4 (E3 ubiquitin ligase BIG BROTHER OS=Momordica charantia OX=3673 GN=LOC111017324 PE=4 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 9.0e-134
Identity = 235/258 (91.09%), Postives = 242/258 (93.80%), Query Frame = 0


Query: 1   MNEDRQMEVHYIDTGFPYTVTESFMDFFEGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60
           MNEDRQMEVHYIDTGFPYT TESFMDFFEGLH HQVPAYGNA PLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSVANVEIPEPTYAPSVEEDVVDAH 120
           KFGVSD GNTYYGHSEESHHLPPSMD+GER WEYP++V  VE PEPTYAPSVEEDVVDAH
Sbjct: 61  KFGVSDPGNTYYGHSEESHHLPPSMDIGERGWEYPSTV-TVENPEPTYAPSVEEDVVDAH 120

Query: 121 SIP-EECDLSHQ-EETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLP 180
            IP EECD SHQ EETSTSQV WQDEIDPDHMTYEELLDLGESVGT+SRGLSEEQISLLP
Sbjct: 121 FIPEEECDFSHQEEETSTSQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLP 180

Query: 181 TARFKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICN 240
           TA+ KLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICN
Sbjct: 181 TAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICN 240

Query: 241 IEVFGDGSMNASGNDNRQ 257
           IEVFGD  +NASG+DNRQ
Sbjct: 241 IEVFGDEGVNASGSDNRQ 257

The following BLAST results are available for this feature:

BLAST of Bhi09G000526 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G63530.1	1.6e-66	49.80	RING/U-box superfamily protein	[more]
AT3G63530.2	1.6e-66	49.80	RING/U-box superfamily protein	[more]
AT3G19910.1	3.2e-27	51.35	RING/U-box superfamily protein	[more]
AT5G52140.1	3.5e-21	35.00	RING/U-box superfamily protein	[more]
AT3G47180.1	1.3e-20	42.97	RING/U-box superfamily protein	[more]

BLAST of Bhi09G000526 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L649	2.2e-65	49.80	E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE...	[more]
Q9LT17	4.6e-26	51.35	E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR P...	[more]
O49500	6.8e-14	40.86	E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV...	[more]
Q7XTV7	2.6e-13	41.94	Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399...	[more]
Q9ZQF9	2.6e-13	44.00	E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV...	[more]

BLAST of Bhi09G000526 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CED1	4.4e-141	94.49	E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034999...	[more]
A0A5D3CGQ8	2.2e-140	94.09	E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3CES7	2.3e-137	94.35	E3 ubiquitin ligase BIG BROTHER isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034999...	[more]
A0A0A0K8C4	3.0e-137	94.35	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428220 P...	[more]
A0A6J1D3R4	9.0e-134	91.09	E3 ubiquitin ligase BIG BROTHER OS=Momordica charantia OX=3673 GN=LOC111017324 P...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 197..237 e-value: 1.7E-4 score: 30.9
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 196..238 e-value: 1.5E-11 score: 44.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 197..238 score: 11.456568
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 159..242 e-value: 1.4E-18 score: 68.3
IPR033276	E3 ubiquitin-protein ligase BIG BROTHER	PANTHER	PTHR46400	RING/U-BOX SUPERFAMILY PROTEIN	coord: 1..247
None	No IPR available	PANTHER	PTHR46400:SF3	E3 UBIQUITIN-PROTEIN LIGASE BIG BROTHER	coord: 1..247
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 192..242

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M000526	Bhi09M000526	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048437	floral organ development
biological_process	GO:0008285	negative regulation of cell population proliferation
biological_process	GO:0046621	negative regulation of organ growth
biological_process	GO:1900057	positive regulation of leaf senescence
biological_process	GO:0051865	protein autoubiquitination
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity