Bhi09G000515 (gene) Wax gourd (B227) v1

Overview
NameBhi09G000515
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionSpoU_methylase domain-containing protein
Locationchr9: 13199833 .. 13203171 (+)
RNA-Seq ExpressionBhi09G000515
SyntenyBhi09G000515
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTCCTCAAAATAACTACAAAACCCGTAAGTTCCCTACCTTCGAAACGAACTTAGAGAGGCACAAAGTATAGAAGAAGAGCGGCAGCAAGAAGGAAGAAGAAGAAGAAGATTGTTCTGCTAAGATGCCATTAATGGACACAGCAACAGTCACTCTCAGAACTTTAAACCCATTTCCCCCTTTTCAACTACGCTCATCGAGGCCTCATCATCTTCTTCGCCCTCACTGCTTCTCCCATTTCACCAACAAACAGCACAATCCATCTTCTTCCTCCTCCTTCCACCGGCTTCTCCCCTTCTGCTCCTTATGTAAGTATCTATCTGTAATTAAATGTTCTTACATCCTATGGACTCTGTAATTTCGGCTGTCAATTGAGTTGAAAGCGCCTTTTTAGTTTGGTTTTGCTGTGTTAATGGCGCAGCTGAAAGAGTGGAGGGTTCTGGTTCCCTTCCGAGTGGTGTTGGTGAGGCCGTTAATGGGGTTTCCCAGCCAAAGCTTCTTCAAGTTGTATTAGTTTCTCCCTTGGTAATTATTTAAACATAGAACAAGTTCCCTTGCTTAGTTTATTCTTTTTCTTGCTCTTCTTTTCTGCATTTTCTTGCAAGCCATCGCTATCTTTAGTTTGTTTGTGTTTCTATCTGTATCTGGGGATTTGTACAGCTTGACTTAACATTTTGAAGAAGTTTCATATCGCTTTAAGCTTTGTTTGATTGTCAAGGACGAAATTTCACATGATTGAAAATTTTGATCAGATGTGAAGTGGCAGTACTTTTAAGAACTTCAGCTGTAACGTGTAGTCAATAGAGTAGGTTAGGTTCAAGAGTTTTTTGGGCATGGACGACCCAAATTTGGCTGTGCAAATGTCAAATAACTAATTCTGAAAACTAACATTTTTCTTAAGTTACAAAGCATCAATGAATGTTAAATTCTCCTTAGAGCGCATTTGCATTCCCGGGTTCGCACCCTTCGCAAATTGACGAGACCCCGAACGTGATATGAAGGTGCATTGTGATTTTATAAGAATAAGAATTCTTGCCCATTATAAAATTTCACGATTTTATGACTTCTATTGAAAGTGAAATCGCATTAGGGTCTCATCAATTCGCGATGGGTGAGACCCGCAATCACAAATGGGCAATTTAAAATTAGCCGTTTCAAAAGGTTAGTATTCATTATTTTTAGAATTCAGGTCATTTGACATTAGTGAGACCAAATTTGGGTCATTCGTACAAAAATCAGCTTAATAAGAAAAAACCTTTAATATCTCTGGGTTCTCGGGATCGGGCTGGGCGTGGTTGCCCCTCAATTAATGGGGGTAAGACCCTTGATACCGGGTTACCAAAACATATAGGGTAGCTTTTCCTAGATTCTGGATGTTCTTTGAAAGGATGTACTCCAGATTTACTTTGAATTTTTCAATAAGACAAACAAAAGAAATTCAGAGGATATTGGCACAGGGAAATTTGCTGACATTCCAGTGGGTGTTTTGTTTGAAAACAAATTAATAGGGACTAGGGAGCCTTTAGTTAGATATTTGCATCAATCTCTGTCGTGTGCATAAGGTTGATGGTAAACTAGAGACCTTAGGTGGTATCATCGGATGATTATCAAGTTGGAATTAGTAGAGCTGCTGGCACTTAATGCCTGATTCGCAACAGTCGTCTTCAAGTTGAGATGTGAGGGTTGAGACAGCATCTTGTCGAAATTTCAATAGAATGCTCGTAAACTTGTCAAGCAGTTGATGTCATATTAGAGCAAAAGGTTGTGTGATGTTAATGGTAAACTTTTATTTTGGTCGATATCAAATGCTTCTAGTATATTTCTTCCAAATTGAATTCTACTTCTAACAAAATACAAAATCCACATTGTGTGTACATACAGTACAACTTATTAGTTTGTATATCATGGCACGAGTTGTATGCAGTTGTGTGAAACACGGTGAATGCCGAATCGTAATTTCTCCATTTTCCTTTTGGGTTGCAGACAACCTCAAAATTCCTCAAGGATGGCTCTGAGATTTCTGTTGTCCTTGACAGATTCCTGGGAACACTGGTAGCATTGCCAGAACATGTGCGGCATCAGCTGTTGGACTACATCTAGTTGGGGTTAGTATTGTAACATCTCTACTTCGAGTGCAATCGTTCTGATTTCCCCCTCAAAAATCTTTTTTTGGAGCTGCTGTTGACTTTAATAACTTATCTTTCTCAGCCATTGGGATTTAAGGTAGATGACACGAAGTTAAAGCGGGCTGGTTTGGATTATTGGCCGTATCCCATGATGCATTCTATACATGATTCTTGTGCTCTCATTTACAGTTAAATAACAGGAATTCGAGTTCTTTGAATGTTGGAAAATTGTACTTTACGTTTTCTTGAACTGTTTGAGTTTTCTATTTCGTGCTGCCACTGTACATATAGTTCAATGTTTTCGAGTTTTAACATCGTATTTTAGATACGTCGTCGTTAAAATTCATCACACGTGGGCAGACTTTCGAGACTACTTTATGAAACAGGTAATCTTCATTGCTTTTGTCTCATGTCTGCATAGAAAATGTGAACAAAATTCTATTTGCTTAGCGATTCTATGAGTTTCTTCCTCTTTGCATCCTGGATTGTTGAGACTATGTCTACCGACCCAATTGATATGATGTATACAGGAAGGAGAAAAGCGGCTGCTAGCATTTACAAAGAGAGGAACTACACTTCACTCGGTGAGTGCCCATTTCTTTGATGTTCTCTATACGGGTCTTATGAAATGTCGATCGCTCATGGGCTAGGGAAGGATTTTGAGTAACAATGGTTGTTCTCTTTTTTCATTTCTGCAGGATTTTTCATACAAGCGGGGCGATTACTTGTTGTTTGGCTCGGAAACTAGTGGCTTACCAGCCGAAGCTCTTGAGGACTGCAAAAACCAGCCATTTGGGGGAGGGACTTTAAGAATTCCAATGGTTGAAACTTATGTTAGATGCCTCAATCTCTCGGTAAGTGTTGGTATTGCCATATATGAAGCTTCAAGACAACTAAATTACGAGCAACTACAGCAGGTTGGATCAGAGAGTTGTACTGGCAATGAACAATCTATCATTACAGAAGATCTTTTTGCTTGATTCTTGGATATGAAATTTTGTACCATTTTAAATTTATTTGGAAACTATAACAGAAACATTGTTTACCCTATGGTCTTAAAGTTGATTCATTGCATGATGCTGTTATATTGCTGTTTTGTTATATTTTGTAATGTTAACCTGTTTTTCAATTCATGATTGTATTTCACCTTACTTTAATGTGACCATAAGAGTTCTTGTAGATTATTATTTGGTTTAAATCCTATTTTGGACTC

mRNA sequence

ATTTCCTCAAAATAACTACAAAACCCGTAAGTTCCCTACCTTCGAAACGAACTTAGAGAGGCACAAAGTATAGAAGAAGAGCGGCAGCAAGAAGGAAGAAGAAGAAGAAGATTGTTCTGCTAAGATGCCATTAATGGACACAGCAACAGTCACTCTCAGAACTTTAAACCCATTTCCCCCTTTTCAACTACGCTCATCGAGGCCTCATCATCTTCTTCGCCCTCACTGCTTCTCCCATTTCACCAACAAACAGCACAATCCATCTTCTTCCTCCTCCTTCCACCGGCTTCTCCCCTTCTGCTCCTTATCTGAAAGAGTGGAGGGTTCTGGTTCCCTTCCGAGTGGTGTTGGTGAGGCCGTTAATGGGGTTTCCCAGCCAAAGCTTCTTCAAGTTGTATTAGTTTCTCCCTTGATTCCTGGGAACACTGGTAGCATTGCCAGAACATGTGCGGCATCAGCTGTTGGACTACATCTAGTTGGGCCATTGGGATTTAAGGTAGATGACACGAAGTTAAAGCGGGCTGGTTTGGATTATTGGCCATACGTCGTCGTTAAAATTCATCACACGTGGGCAGACTTTCGAGACTACTTTATGAAACAGGAAGGAGAAAAGCGGCTGCTAGCATTTACAAAGAGAGGAACTACACTTCACTCGGATTTTTCATACAAGCGGGGCGATTACTTGTTGTTTGGCTCGGAAACTAGTGGCTTACCAGCCGAAGCTCTTGAGGACTGCAAAAACCAGCCATTTGGGGGAGGGACTTTAAGAATTCCAATGGTTGAAACTTATGTTAGATGCCTCAATCTCTCGGTAAGTGTTGGTATTGCCATATATGAAGCTTCAAGACAACTAAATTACGAGCAACTACAGCAGGTTGGATCAGAGAGTTGTACTGGCAATGAACAATCTATCATTACAGAAGATCTTTTTGCTTGATTCTTGGATATGAAATTTTGTACCATTTTAAATTTATTTGGAAACTATAACAGAAACATTGTTTACCCTATGGTCTTAAAGTTGATTCATTGCATGATGCTGTTATATTGCTGTTTTGTTATATTTTGTAATGTTAACCTGTTTTTCAATTCATGATTGTATTTCACCTTACTTTAATGTGACCATAAGAGTTCTTGTAGATTATTATTTGGTTTAAATCCTATTTTGGACTC

Coding sequence (CDS)

ATGCCATTAATGGACACAGCAACAGTCACTCTCAGAACTTTAAACCCATTTCCCCCTTTTCAACTACGCTCATCGAGGCCTCATCATCTTCTTCGCCCTCACTGCTTCTCCCATTTCACCAACAAACAGCACAATCCATCTTCTTCCTCCTCCTTCCACCGGCTTCTCCCCTTCTGCTCCTTATCTGAAAGAGTGGAGGGTTCTGGTTCCCTTCCGAGTGGTGTTGGTGAGGCCGTTAATGGGGTTTCCCAGCCAAAGCTTCTTCAAGTTGTATTAGTTTCTCCCTTGATTCCTGGGAACACTGGTAGCATTGCCAGAACATGTGCGGCATCAGCTGTTGGACTACATCTAGTTGGGCCATTGGGATTTAAGGTAGATGACACGAAGTTAAAGCGGGCTGGTTTGGATTATTGGCCATACGTCGTCGTTAAAATTCATCACACGTGGGCAGACTTTCGAGACTACTTTATGAAACAGGAAGGAGAAAAGCGGCTGCTAGCATTTACAAAGAGAGGAACTACACTTCACTCGGATTTTTCATACAAGCGGGGCGATTACTTGTTGTTTGGCTCGGAAACTAGTGGCTTACCAGCCGAAGCTCTTGAGGACTGCAAAAACCAGCCATTTGGGGGAGGGACTTTAAGAATTCCAATGGTTGAAACTTATGTTAGATGCCTCAATCTCTCGGTAAGTGTTGGTATTGCCATATATGAAGCTTCAAGACAACTAAATTACGAGCAACTACAGCAGGTTGGATCAGAGAGTTGTACTGGCAATGAACAATCTATCATTACAGAAGATCTTTTTGCTTGA

Protein sequence

MPLMDTATVTLRTLNPFPPFQLRSSRPHHLLRPHCFSHFTNKQHNPSSSSSFHRLLPFCSLSERVEGSGSLPSGVGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIHHTWADFRDYFMKQEGEKRLLAFTKRGTTLHSDFSYKRGDYLLFGSETSGLPAEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNYEQLQQVGSESCTGNEQSIITEDLFA
Homology
[Close]
BLAST of Bhi09G000515 vs. TAIR 10
Match: AT5G26880.2 (AGAMOUS-like 26 )

HSP 1 Score: 312.0 bits (798), Expect = 4.7e-85
Identity = 146/200 (73.00%), Postives = 171/200 (85.50%), Query Frame = 0 

Query: 71  LPSGVGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKL 130
           LP G GE V   ++ KLL VVLVSP IPGNTG IARTCAASAVGLHLVGPLGF+VDD ++
Sbjct: 65  LPRGAGEGVKEDARSKLLHVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGFQVDDARV 124

Query: 131 KRAGLDYWPYVVVKIHHTWADFRDYFMKQEGEKRLLAFTKRGTTLHSDFSYKRGDYLLFG 190
           KRAGLDYWP+VVVK H +WA+F++YF  QEGEKR++AFTKRGT +HSDFSY+ GDYLLFG
Sbjct: 125 KRAGLDYWPFVVVKAHSSWAEFQEYFRLQEGEKRMIAFTKRGTRIHSDFSYRSGDYLLFG 184

Query: 191 SETSGLPAEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNYEQLQQ 250
           SETSGLP EAL DC ++P+GGGTLRIPMVETYVRCLNLSVSVGIA+YEASRQLNYEQ+ +
Sbjct: 185 SETSGLPPEALSDCNHEPYGGGTLRIPMVETYVRCLNLSVSVGIALYEASRQLNYEQI-E 244

Query: 251 VGSESCTGNEQSIITEDLFA 271
              + C   E+ ++TED+FA
Sbjct: 245 CAPQGCVNGEEPLLTEDIFA 263

[Close]
BLAST of Bhi09G000515 vs. TAIR 10
Match: AT5G26880.1 (AGAMOUS-like 26 )

HSP 1 Score: 265.8 bits (678), Expect = 3.9e-71
Identity = 122/160 (76.25%), Postives = 140/160 (87.50%), Query Frame = 0 

Query: 71  LPSGVGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKL 130
           LP G GE V   ++ KLL VVLVSP IPGNTG IARTCAASAVGLHLVGPLGF+VDD ++
Sbjct: 65  LPRGAGEGVKEDARSKLLHVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGFQVDDARV 124

Query: 131 KRAGLDYWPYVVVKIHHTWADFRDYFMKQEGEKRLLAFTKRGTTLHSDFSYKRGDYLLFG 190
           KRAGLDYWP+VVVK H +WA+F++YF  QEGEKR++AFTKRGT +HSDFSY+ GDYLLFG
Sbjct: 125 KRAGLDYWPFVVVKAHSSWAEFQEYFRLQEGEKRMIAFTKRGTRIHSDFSYRSGDYLLFG 184

Query: 191 SETSGLPAEALEDCKNQPFGGGTLRIPMVETYVRCLNLSV 231
           SETSGLP EAL DC ++P+GGGTLRIPMVETYVRCLNLS+
Sbjct: 185 SETSGLPPEALSDCNHEPYGGGTLRIPMVETYVRCLNLSI 224

[Close]
BLAST of Bhi09G000515 vs. ExPASy Swiss-Prot
Match: P74516 (Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr0992 PE=3 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 4.1e-33
Identity = 77/157 (49.04%), Postives = 104/157 (66.24%), Query Frame = 0 

Query: 87  LLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIH 146
           ++++VLV+P IP NTG+IARTCAA+   LHLVGPLGF++ D  LKRAGLDYWPYV +  H
Sbjct: 1   MVKLVLVNPQIPPNTGNIARTCAATGTELHLVGPLGFELGDRYLKRAGLDYWPYVDLHYH 60

Query: 147 HTWADFRDYFMKQEGEKRLLAFTKRGTTLHSDFSYKRGDYLLFGSETSGLPAEALEDCKN 206
           ++  +F  ++  Q G   LL F+  GT    D++Y+  D+L+ GSET GLP + + D   
Sbjct: 61  NSCEEFITHWRGQGG--NLLGFSVTGTVNFWDYTYQSTDWLMMGSETDGLP-QMMRDLST 120

Query: 207 QPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQL 244
           Q      L IPM  + VR LNL+ S  IA++EA RQL
Sbjct: 121 Q-----LLCIPMPHSEVRSLNLASSAAIALFEAGRQL 149

[Close]
BLAST of Bhi09G000515 vs. ExPASy Swiss-Prot
Match: P0AGJ8 (tRNA (cytidine(34)-2'-O)-methyltransferase OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=trmL PE=3 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 8.5e-31
Identity = 74/159 (46.54%), Postives = 98/159 (61.64%), Query Frame = 0 

Query: 87  LLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIH 146
           +L +VL  P IP NTG+I R CA +   LH++ P+GF  DD +L+RAGLDY  +  V  H
Sbjct: 1   MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRH 60

Query: 147 HTWADFRDYFMKQEGEKRLLAFTKRGTTLHSDFSYKRGDYLLFGSETSGLPAEALEDCKN 206
           H   D+R  F++ E  +RL A T +GT  HS  SY+ GDYL+FG ET GLPA  L+    
Sbjct: 61  H---DYR-AFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPA 120

Query: 207 QPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 246
           +      +RIPMV    R +NLS +V + +YEA RQL Y
Sbjct: 121 EQ----KIRIPMVPD-SRSMNLSNAVSVVVYEAWRQLGY 150

[Close]
BLAST of Bhi09G000515 vs. ExPASy Swiss-Prot
Match: P0AGJ7 (tRNA (cytidine(34)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=trmL PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 8.5e-31
Identity = 74/159 (46.54%), Postives = 98/159 (61.64%), Query Frame = 0 

Query: 87  LLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIH 146
           +L +VL  P IP NTG+I R CA +   LH++ P+GF  DD +L+RAGLDY  +  V  H
Sbjct: 1   MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRH 60

Query: 147 HTWADFRDYFMKQEGEKRLLAFTKRGTTLHSDFSYKRGDYLLFGSETSGLPAEALEDCKN 206
           H   D+R  F++ E  +RL A T +GT  HS  SY+ GDYL+FG ET GLPA  L+    
Sbjct: 61  H---DYR-AFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPA 120

Query: 207 QPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 246
           +      +RIPMV    R +NLS +V + +YEA RQL Y
Sbjct: 121 EQ----KIRIPMVPD-SRSMNLSNAVSVVVYEAWRQLGY 150

[Close]
BLAST of Bhi09G000515 vs. ExPASy Swiss-Prot
Match: C7BSD3 (tRNA (cytidine(34)-2'-O)-methyltransferase OS=Photorhabdus asymbiotica subsp. asymbiotica (strain ATCC 43949 / 3105-77) OX=553480 GN=trmL PE=3 SV=2)

HSP 1 Score: 133.7 bits (335), Expect = 3.2e-30
Identity = 73/160 (45.62%), Postives = 97/160 (60.62%), Query Frame = 0 

Query: 87  LLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIH 146
           +L +VL  P IP NTG+I R CA +   LHL+ PLGF  DD +L+RAGLDY  +  +K H
Sbjct: 1   MLNIVLFEPEIPPNTGNIIRLCANTGFQLHLIQPLGFTWDDKRLRRAGLDYHEFANIKQH 60

Query: 147 HTWADF-RDYFMKQEGEKRLLAFTKRGTTLHSDFSYKRGDYLLFGSETSGLPAEALEDCK 206
           H +  F  +  +  E   RL A T +GT  HS  SY+ GDYL+FG ET GLP+  L++  
Sbjct: 61  HDYHSFLENEGVTGESFARLFALTTKGTPAHSAVSYQAGDYLMFGPETRGLPSYVLDNMP 120

Query: 207 NQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 246
            Q      +RIPM+    R +NLS SV + ++EA RQL Y
Sbjct: 121 PQQ----KIRIPMLAD-SRSMNLSNSVAVVVFEAWRQLGY 155

[Close]
BLAST of Bhi09G000515 vs. ExPASy Swiss-Prot
Match: P44868 (tRNA (cytidine(34)-2'-O)-methyltransferase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=trmL PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 9.4e-30
Identity = 70/160 (43.75%), Postives = 95/160 (59.38%), Query Frame = 0 

Query: 87  LLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIH 146
           +L +VL  P IP NTG+I R CA +   LHL+ PLGF  DD +L+R+GLDY  +  +K H
Sbjct: 1   MLDIVLYEPEIPQNTGNIIRLCANTGFRLHLIEPLGFTWDDKRLRRSGLDYHEFAEIKRH 60

Query: 147 HTWADFRDYFMKQEGEKRLLAFTKRGTTLHSDFSYKRGDYLLFGSETSGLPAEALEDCKN 206
            T+    + F++ E  KRL A T +G   HS   +K GDYL+FG ET G+P   L +   
Sbjct: 61  KTF----EAFLESEKPKRLFALTTKGCPAHSQVKFKLGDYLMFGPETRGIPMSILNEMPM 120

Query: 207 QPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNYE 247
           +      +RIPM     R +NLS SV + +YEA RQL Y+
Sbjct: 121 EQ----KIRIPMTAN-SRSMNLSNSVAVTVYEAWRQLGYK 151

[Close]
BLAST of Bhi09G000515 vs. ExPASy TrEMBL
Match: A0A5D3CFA4 (Putative tRNA (Cytidine(34)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001000 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 7.2e-134
Identity = 245/270 (90.74%), Postives = 252/270 (93.33%), Query Frame = 0 

Query: 1   MPLMDTATVTLRTLNPFPPFQLRSSRPHHLLRPHCFSHFTNKQHNPSSSSSFHRLLPFCS 60
           MPLMDT T TLRTLNPF PFQ RSSR HHLLRPH FS FTNK HNPSSSSS  RLLPFCS
Sbjct: 49  MPLMDTTTATLRTLNPFTPFQRRSSRSHHLLRPHSFSLFTNKHHNPSSSSS-RRLLPFCS 108

Query: 61  LSERVEGSGSLPSGVGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGP 120
            SERVEGSGSLPSGVGEAVNGVS PK+LQVVLVSPLIPGNTGSIARTCAASAVGLHLVGP
Sbjct: 109 SSERVEGSGSLPSGVGEAVNGVSHPKILQVVLVSPLIPGNTGSIARTCAASAVGLHLVGP 168

Query: 121 LGFKVDDTKLKRAGLDYWPYVVVKIHHTWADFRDYFMKQEGEKRLLAFTKRGTTLHSDFS 180
           LGFKVDDTKLKRAGLDYWPYVVVKIH+TWADF++YF KQEGEKRLLAFTKRGTT HSDFS
Sbjct: 169 LGFKVDDTKLKRAGLDYWPYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFS 228

Query: 181 YKRGDYLLFGSETSGLPAEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEAS 240
           YKRGDYLLFGSET+GLP EALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEAS
Sbjct: 229 YKRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEAS 288

Query: 241 RQLNYEQLQQVGSESCTGNEQSIITEDLFA 271
           RQLNYEQL QV SESCT +EQSIITED+FA
Sbjct: 289 RQLNYEQL-QVSSESCTNDEQSIITEDVFA 316

[Close]
BLAST of Bhi09G000515 vs. ExPASy TrEMBL
Match: A0A1S3CFJ1 (putative tRNA (Cytidine(34)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499907 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 7.2e-134
Identity = 245/270 (90.74%), Postives = 252/270 (93.33%), Query Frame = 0 

Query: 1   MPLMDTATVTLRTLNPFPPFQLRSSRPHHLLRPHCFSHFTNKQHNPSSSSSFHRLLPFCS 60
           MPLMDT T TLRTLNPF PFQ RSSR HHLLRPH FS FTNK HNPSSSSS  RLLPFCS
Sbjct: 1   MPLMDTTTATLRTLNPFTPFQRRSSRSHHLLRPHSFSLFTNKHHNPSSSSS-RRLLPFCS 60

Query: 61  LSERVEGSGSLPSGVGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGP 120
            SERVEGSGSLPSGVGEAVNGVS PK+LQVVLVSPLIPGNTGSIARTCAASAVGLHLVGP
Sbjct: 61  SSERVEGSGSLPSGVGEAVNGVSHPKILQVVLVSPLIPGNTGSIARTCAASAVGLHLVGP 120

Query: 121 LGFKVDDTKLKRAGLDYWPYVVVKIHHTWADFRDYFMKQEGEKRLLAFTKRGTTLHSDFS 180
           LGFKVDDTKLKRAGLDYWPYVVVKIH+TWADF++YF KQEGEKRLLAFTKRGTT HSDFS
Sbjct: 121 LGFKVDDTKLKRAGLDYWPYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFS 180

Query: 181 YKRGDYLLFGSETSGLPAEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEAS 240
           YKRGDYLLFGSET+GLP EALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEAS
Sbjct: 181 YKRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEAS 240

Query: 241 RQLNYEQLQQVGSESCTGNEQSIITEDLFA 271
           RQLNYEQL QV SESCT +EQSIITED+FA
Sbjct: 241 RQLNYEQL-QVSSESCTNDEQSIITEDVFA 268

[Close]
BLAST of Bhi09G000515 vs. ExPASy TrEMBL
Match: A0A0A0K6L9 (SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428850 PE=3 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.2e-130
Identity = 241/270 (89.26%), Postives = 248/270 (91.85%), Query Frame = 0 

Query: 1   MPLMDTATVTLRTLNPFPPFQLRSSRPHHLLRPHCFSHFTNKQHNPSSSSSFHRLLPFCS 60
           MPLMDT T TLR LNPF PFQLRSSR  HLLRP  FS FTNK HN  SSSS  RLLPFCS
Sbjct: 1   MPLMDTTTATLRALNPFTPFQLRSSRSLHLLRPRSFSLFTNKHHN-LSSSSLRRLLPFCS 60

Query: 61  LSERVEGSGSLPSGVGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGP 120
            S+RVEGSGSLP+GVGEAVNGVS PKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGP
Sbjct: 61  SSDRVEGSGSLPTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGP 120

Query: 121 LGFKVDDTKLKRAGLDYWPYVVVKIHHTWADFRDYFMKQEGEKRLLAFTKRGTTLHSDFS 180
           LGFKVDDTKLKRAGLDYWPYVVVKIH+TWADF++YF KQEGEKRLLAFTKRGTT HSDFS
Sbjct: 121 LGFKVDDTKLKRAGLDYWPYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFS 180

Query: 181 YKRGDYLLFGSETSGLPAEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEAS 240
           YKRGDYLLFGSET+GLP EALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEAS
Sbjct: 181 YKRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEAS 240

Query: 241 RQLNYEQLQQVGSESCTGNEQSIITEDLFA 271
           RQLNYEQL QV SESCT NEQSIITED+FA
Sbjct: 241 RQLNYEQL-QVSSESCTNNEQSIITEDVFA 268

[Close]
BLAST of Bhi09G000515 vs. ExPASy TrEMBL
Match: A0A6J1KVT5 (uncharacterized protein LOC111498049 OS=Cucurbita maxima OX=3661 GN=LOC111498049 PE=3 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 2.2e-122
Identity = 231/272 (84.93%), Postives = 243/272 (89.34%), Query Frame = 0 

Query: 1   MPLMDTATVTLRTLNPFPPFQLRSSRPHHLLRPHCFSHFTNKQHNPSSSSSFHRLLPFCS 60
           MPLMDT   TLRTL+PF PF LRSS  H LLR  CFSHF++K  +P SSSS  RLLPFCS
Sbjct: 50  MPLMDTTATTLRTLHPFSPFLLRSSTSHRLLRARCFSHFSHKHRHPCSSSS-RRLLPFCS 109

Query: 61  LSERVEG-SGSLPSGVGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVG 120
            SE+ EG SGSLP GVGEA+NGVSQ K+LQVVLVSPLIPGNTGSIARTCAASAVGLHLVG
Sbjct: 110 FSEKGEGSSGSLPRGVGEALNGVSQQKVLQVVLVSPLIPGNTGSIARTCAASAVGLHLVG 169

Query: 121 PLGFKVDDTKLKRAGLDYWPYVVVKIHHTWADFRDYFMKQEGEKRLLAFTKRGTTLHSDF 180
           PLGFKVDDTKLKRAGLDYWPYVVVKIH+TWA+FRDYF KQEGEKRLLAFTKRGTTLHSDF
Sbjct: 170 PLGFKVDDTKLKRAGLDYWPYVVVKIHNTWAEFRDYFKKQEGEKRLLAFTKRGTTLHSDF 229

Query: 181 SYKRGDYLLFGSETSGLPAEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA 240
           SYK+GDYLLF SETSGLPAEALEDCKNQ FGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
Sbjct: 230 SYKQGDYLLFRSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA 289

Query: 241 SRQLNYEQLQQVGSESCT-GNEQSIITEDLFA 271
           SRQLNY+QL Q+  E CT  NEQSIITEDLFA
Sbjct: 290 SRQLNYQQL-QIEPEGCTNNNEQSIITEDLFA 319

[Close]
BLAST of Bhi09G000515 vs. ExPASy TrEMBL
Match: A0A6J1H4X6 (uncharacterized protein LOC111460609 OS=Cucurbita moschata OX=3662 GN=LOC111460609 PE=3 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.4e-121
Identity = 229/272 (84.19%), Postives = 243/272 (89.34%), Query Frame = 0 

Query: 1   MPLMDTATVTLRTLNPFPPFQLRSSRPHHLLRPHCFSHFTNKQHNPSSSSSFHRLLPFCS 60
           MPLMDT   TLRTL+PF PF LR+S  H LLR  CFSHF++K  +P SSSS  RLLPFCS
Sbjct: 1   MPLMDTTATTLRTLHPFSPFLLRASTSHRLLRARCFSHFSHKHRHPCSSSS-RRLLPFCS 60

Query: 61  LSERVEG-SGSLPSGVGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVG 120
            SE+ EG SGSLP GVG+A+NGVSQ K+LQVVLVSPLIPGNTGSIARTCAASAVGLHLVG
Sbjct: 61  FSEKGEGSSGSLPRGVGKALNGVSQQKVLQVVLVSPLIPGNTGSIARTCAASAVGLHLVG 120

Query: 121 PLGFKVDDTKLKRAGLDYWPYVVVKIHHTWADFRDYFMKQEGEKRLLAFTKRGTTLHSDF 180
           PLGFKVDD KLKRAGLDYWPYVVVKIH+TWA+FRDYF KQEGEKRLLAFTKRGTTLHSDF
Sbjct: 121 PLGFKVDDKKLKRAGLDYWPYVVVKIHNTWAEFRDYFKKQEGEKRLLAFTKRGTTLHSDF 180

Query: 181 SYKRGDYLLFGSETSGLPAEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA 240
           SYK+GDYLLFGSETSGLPAEALEDCKNQ FGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
Sbjct: 181 SYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA 240

Query: 241 SRQLNYEQLQQVGSESCT-GNEQSIITEDLFA 271
           SRQLNY+QL Q+  E CT  NEQSIITEDLFA
Sbjct: 241 SRQLNYQQL-QLEPEGCTNNNEQSIITEDLFA 270

The following BLAST results are available for this feature:
BLAST of Bhi09G000515 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G26880.24.7e-8573.00AGAMOUS-like 26 [more]
AT5G26880.13.9e-7176.25AGAMOUS-like 26 [more]
BLAST of Bhi09G000515 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P745164.1e-3349.04Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Synechocystis sp. (strain... [more]
P0AGJ88.5e-3146.54tRNA (cytidine(34)-2'-O)-methyltransferase OS=Escherichia coli O6:H1 (strain CFT... [more]
P0AGJ78.5e-3146.54tRNA (cytidine(34)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=8... [more]
C7BSD33.2e-3045.63tRNA (cytidine(34)-2'-O)-methyltransferase OS=Photorhabdus asymbiotica subsp. as... [more]
P448689.4e-3043.75tRNA (cytidine(34)-2'-O)-methyltransferase OS=Haemophilus influenzae (strain ATC... [more]
BLAST of Bhi09G000515 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3CFA47.2e-13490.74Putative tRNA (Cytidine(34)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa ... [more]
A0A1S3CFJ17.2e-13490.74putative tRNA (Cytidine(34)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=L... [more]
A0A0A0K6L92.2e-13089.26SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428... [more]
A0A6J1KVT52.2e-12284.93uncharacterized protein LOC111498049 OS=Cucurbita maxima OX=3661 GN=LOC111498049... [more]
A0A6J1H4X61.4e-12184.19uncharacterized protein LOC111460609 OS=Cucurbita moschata OX=3662 GN=LOC1114606... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001537tRNA/rRNA methyltransferase, SpoU typePFAMPF00588SpoU_methylasecoord: 88..237
e-value: 1.9E-29
score: 102.6
IPR029026tRNA (guanine-N1-)-methyltransferase, N-terminalGENE3D3.40.1280.10coord: 81..251
e-value: 2.6E-42
score: 146.5
NoneNo IPR availablePIRSRPIRSR029256-1PIRSR029256-1coord: 88..245
e-value: 3.4E-45
score: 151.0
IPR016914tRNA (cytidine/uridine-2'-O-)-methyltransferasePANTHERPTHR42971TRNA (CYTIDINE(34)-2'-O)-METHYLTRANSFERASEcoord: 38..268
IPR016914tRNA (cytidine/uridine-2'-O-)-methyltransferaseHAMAPMF_01885tRNA_methyltr_TrmLcoord: 88..246
score: 31.419598
IPR016914tRNA (cytidine/uridine-2'-O-)-methyltransferaseCDDcd18094SpoU-like_TrmLcoord: 89..243
e-value: 7.63824E-80
score: 235.425
IPR029028Alpha/beta knot methyltransferasesSUPERFAMILY75217alpha/beta knotcoord: 88..249

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi09M000515Bhi09M000515mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002131 wobble position cytosine ribose methylation
biological_process GO:0002132 wobble position uridine ribose methylation
biological_process GO:0001510 RNA methylation
biological_process GO:0006396 RNA processing
molecular_function GO:0003723 RNA binding
molecular_function GO:0008173 RNA methyltransferase activity
molecular_function GO:0008168 methyltransferase activity