Bhi09G000462 (gene) Wax gourd (B227) v1

BLAST of Bhi09G000462 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 741.9 bits (1914), Expect = 3.7e-214
Identity = 364/539 (67.53%), Postives = 449/539 (83.30%), Query Frame = 0

Query: 8   TNEFIFRSKLPDIHIPNHLPLHDYIFQNLPKFASRPCLINGATGDVYSFHDVQLTARRVA 67
           +N+ IFRS+LPDI+IPNHLPLHDYIF+N+ +FA++PCLING TG+VY++ DV +T+R++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  AGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAK 127
           AGLHNLG+K+ DVVM LLPNSPE V TFL AS+ GAI T+ANPF+T  EI+KQAKA+ AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 LIVTMACFYDRVKDLAENGVKIVCVDFAV--EGCLHFSVLSGADEAHAPAV--DFSPDDV 187
           LIVT + + D++K+L  +GV IV  D     E CL FS L+ ++E    ++    SP+DV
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDV 197

Query: 188 VALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYSLNS 247
           VALP+SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+  DDVILCVLP FHIY+LNS
Sbjct: 198 VALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNS 257

Query: 248 ILLCGIRAGAAIMIMQKFDIVSLFQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVSSVR 307
           I+LC +R GA I+IM KF+I  L + I++ ++++  +VPPI LAIAKS E EKYD+SSVR
Sbjct: 258 IMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVR 317

Query: 308 VLKSGGAPLGKELEDAVRAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAGACGTV 367
           ++KSG APLGKELEDA+ AKFP A L QGYGMTEAGPVL MSL FAKEPF VK+GACGTV
Sbjct: 318 MVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTV 377

Query: 368 VRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGF 427
           VRNAEMKI+DP+TG SLP N  GEICIRG+QIMKGYLND  +T  TIDK+GWLHTGD+GF
Sbjct: 378 VRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGF 437

Query: 428 I-DDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMADEEAGEVPVAFV 487
           I DDDELFIVDRLKELIK+K FQVAPAELE+LLI HP+++D AV+ M +E+AGEVPVAFV
Sbjct: 438 IDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFV 497

Query: 488 VKESDGTITEEEVKHFITKQVVFYKRLKSVYFVNAIPKAPSGKILRKELRTKLASGAYN 542
           V+  D  I+E+E+K F++KQVVFYKR+  V+F ++IPKAPSGKILRK+LR +LA+G  N
Sbjct: 498 VRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLMN 556

BLAST of Bhi09G000462 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 740.3 bits (1910), Expect = 1.1e-213
Identity = 370/544 (68.01%), Postives = 443/544 (81.43%), Query Frame = 0

Query: 5   SNQTNEFIFRSKLPDIHIPNHLPLHDYIFQNLPKFASRPCLINGATGDVYSFHDVQLTAR 64
           +N  ++ IFRSKLPDI+IPNHL LHDYIFQN+ +FA++PCLING TG VY++ DV + +R
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAA 124
           ++AA  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF+T  EIAKQAKA+
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 NAKLIVTMACFYDRVKDLA-ENGVKIVCVDF-----AVEGCLHFSVLSGADEAHAPAVD- 184
           N KLI+T A + D++K L  ++GV IVC+D        EGCL F+ L+ +    +  +D 
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 196

Query: 185 --FSPDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPF 244
              SPDDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+  DDVILCVLP 
Sbjct: 197 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 256

Query: 245 FHIYSLNSILLCGIRAGAAIMIMQKFDIVSLFQLIEKYRISIMPIVPPIFLAIAKSSEFE 304
           FHIY+LNSI+LCG+R GAAI+IM KF+I  L +LI++ ++++ P+VPPI LAIAKSSE E
Sbjct: 257 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 316

Query: 305 KYDVSSVRVLKSGGAPLGKELEDAVRAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQV 364
           KYD+SS+RV+KSG APLGKELEDAV AKFP A L QGYGMTEAGPVL MSL FAKEPF V
Sbjct: 317 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 376

Query: 365 KAGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGW 424
           K+GACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GW
Sbjct: 377 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 436

Query: 425 LHTGDIGFI-DDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMADEEA 484
           LHTGDIG I DDDELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AV+ M +E A
Sbjct: 437 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAA 496

Query: 485 GEVPVAFVVKESDGTITEEEVKHFITKQVVFYKRLKSVYFVNAIPKAPSGKILRKELRTK 539
           GEVPVAFVVK  D  ++E++VK F++KQVVFYKR+  V+F  +IPKAPSGKILRK+LR K
Sbjct: 497 GEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAK 556

BLAST of Bhi09G000462 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 689.1 bits (1777), Expect = 2.8e-198
Identity = 345/509 (67.78%), Postives = 413/509 (81.14%), Query Frame = 0

Query: 5   SNQTNEFIFRSKLPDIHIPNHLPLHDYIFQNLPKFASRPCLINGATGDVYSFHDVQLTAR 64
           +N  ++ IFRSKLPDI+IPNHL LHDYIFQN+ +FA++PCLING TG VY++ DV + +R
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAA 124
           ++AA  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF+T  EIAKQAKA+
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 NAKLIVTMACFYDRVKDLA-ENGVKIVCVDF-----AVEGCLHFSVLSGADEAHAPAVD- 184
           N KLI+T A + D++K L  ++GV IVC+D        EGCL F+ L+ +    +  +D 
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 196

Query: 185 --FSPDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPF 244
              SPDDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+  DDVILCVLP 
Sbjct: 197 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 256

Query: 245 FHIYSLNSILLCGIRAGAAIMIMQKFDIVSLFQLIEKYRISIMPIVPPIFLAIAKSSEFE 304
           FHIY+LNSI+LCG+R GAAI+IM KF+I  L +LI++ ++++ P+VPPI LAIAKSSE E
Sbjct: 257 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 316

Query: 305 KYDVSSVRVLKSGGAPLGKELEDAVRAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQV 364
           KYD+SS+RV+KSG APLGKELEDAV AKFP A L QGYGMTEAGPVL MSL FAKEPF V
Sbjct: 317 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 376

Query: 365 KAGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGW 424
           K+GACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GW
Sbjct: 377 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 436

Query: 425 LHTGDIGFI-DDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMADEEA 484
           LHTGDIG I DDDELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AV+ M +E A
Sbjct: 437 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAA 496

Query: 485 GEVPVAFVVKESDGTITEEEVKHFITKQV 504
           GEVPVAFVVK  D  ++E++VK F++KQV
Sbjct: 497 GEVPVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of Bhi09G000462 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 666.0 bits (1717), Expect = 2.6e-191
Identity = 327/546 (59.89%), Postives = 419/546 (76.74%), Query Frame = 0

Query: 4   ESNQTNEFIFRSKLPDIHIPNHLPLHDYIFQNL----PKFASRPCLINGATGDVYSFHDV 63
           E   +++FIFRSKLPDI IPNHLPL DY+FQ         +S  C+I+GATG + ++ DV
Sbjct: 20  EEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADV 79

Query: 64  QLTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAK 123
           Q   RR+AAG+H LGI+ GDVVM LLPNSPEF  +FL  +Y GA+ T ANPFYT  EIAK
Sbjct: 80  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 139

Query: 124 QAKAANAKLIVTMACFYDRVKDLAENGVKIVCVD---------FAVEGCLHFSVLSGADE 183
           QAKA+ AK+I+T  C  D++ +L  +GV IVC+D          + +GC+ F+ L+ ADE
Sbjct: 140 QAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADE 199

Query: 184 AHAPAVDFSPDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVIL 243
                   SP+D VA+PYSSGTTGLPKGVM+THKGL+TS+AQ++DG+NPNL +  +DVIL
Sbjct: 200 TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVIL 259

Query: 244 CVLPFFHIYSLNSILLCGIRAGAAIMIMQKFDIVSLFQLIEKYRISIMPIVPPIFLAIAK 303
           C LP FHIY+L++++L  +R GAA++I+ +F++  + +LI++Y+++++P+ PP+ LA  K
Sbjct: 260 CFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIK 319

Query: 304 SSEFEKYDVSSVRVLKSGGAPLGKELEDAVRAKFPAAILAQGYGMTEAGPVLTMSLAFAK 363
           S E E+YD+SSVR++ SG A L KELEDAVR KFP AI  QGYGMTE+G V   SLAFAK
Sbjct: 320 SPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAK 379

Query: 364 EPFQVKAGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTI 423
            PF+ K+GACGTV+RNAEMK+VD ETG SLP N +GEIC+RG Q+MKGYLND E+T RTI
Sbjct: 380 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 439

Query: 424 DKEGWLHTGDIGFI-DDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGM 483
           DK+GWLHTGDIGF+ DDDE+FIVDRLKELIKFK +QVAPAELEALLI+HP + DAAV+ M
Sbjct: 440 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 499

Query: 484 ADEEAGEVPVAFVVKESDGTITEEEVKHFITKQVVFYKRLKSVYFVNAIPKAPSGKILRK 536
            DE A EVPVAFV +     +TE++VK ++ KQVV YKR+K V+F+  IPKA SGKILRK
Sbjct: 500 KDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRK 559

BLAST of Bhi09G000462 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 648.3 bits (1671), Expect = 5.5e-186
Identity = 325/527 (61.67%), Postives = 405/527 (76.85%), Query Frame = 0

Query: 12  IFRSKLPDIHIPNHLPLHDYIFQNLPKFASRPCLINGATGDVYSFHDVQLTARRVAAGLH 71
           IFRSKLPDI IPNHLPLH Y F+ L   + +PCLI G+TG  Y++ +  L  RRVA+GL+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  NLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLIVT 131
            LGI+KGDV+M LL NS EFVF+F+GAS  GA+ T ANPFYT+ E+ KQ K++ AKLI+T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 MACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADEAH--APAVDFSPDDVVALPYS 191
            + + D++K+L EN   I   +   E CL FS L   DE +     VD   DD  ALP+S
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALPFS 213

Query: 192 SGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYSLNSILLCGI 251
           SGTTGLPKGV+LTHK LITSVAQQ+DG NPNLY + +DVILCVLP FHIYSLNS+LL  +
Sbjct: 214 SGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSL 273

Query: 252 RAGAAIMIMQKFDIVSLFQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVSSVRVLKSGG 311
           R+GA +++M KF+I +L  LI+++R++I  +VPP+ +A+AK+     YD+SSVR + SG 
Sbjct: 274 RSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSGA 333

Query: 312 APLGKELEDAVRAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAGACGTVVRNAEM 371
           APLGKEL+D++R + P AIL QGYGMTEAGPVL+MSL FAKEP   K+G+CGTVVRNAE+
Sbjct: 334 APLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAEL 393

Query: 372 KIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFID-DDE 431
           K+V  ET  SL  N  GEICIRG QIMK YLND E+T  TID+EGWLHTGDIG++D DDE
Sbjct: 394 KVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDE 453

Query: 432 LFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMADEEAGEVPVAFVVKESDG 491
           +FIVDRLKE+IKFK FQV PAELE+LLI H  ++DAAV+   DE AGEVPVAFVV+ +  
Sbjct: 454 IFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGN 513

Query: 492 TITEEEVKHFITKQVVFYKRLKSVYFVNAIPKAPSGKILRKELRTKL 536
            ITEE+VK ++ KQVVFYKRL  V+FV +IPK+PSGKILRK+L+ KL
Sbjct: 514 DITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of Bhi09G000462 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 1.8e-226
Identity = 386/534 (72.28%), Postives = 454/534 (85.02%), Query Frame = 0

Query: 10  EFIFRSKLPDIHIPNHLPLHDYIFQNLPKFASRPCLINGATGDVYSFHDVQLTARRVAAG 69
           + IFRSKLPDI+IPNHLPLH Y F+N+ +F+SRPCLINGA   +Y++ DV+L +R+VAAG
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 70  LHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLI 129
           LH  GI+  D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA++AK+I
Sbjct: 69  LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 130 VTMACFYDRVKDLA-ENGVKIVCVDFAVEGCLHFSVLSGADEAHAPAVDFSPDDVVALPY 189
           VT AC  ++VKD A EN VKI+C+D A EGCLHFSVL+ A+E   P V+  PDDVVALPY
Sbjct: 129 VTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVALPY 188

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY   +DV+LCVLP FHIYSLNS+LLCG
Sbjct: 189 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCG 248

Query: 250 IRAGAAIMIMQKFDIVSLFQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVSSVRVLKSG 309
           +R GAAI+IMQKFDIVS  +LI++Y+++I P VPPI LAIAKS   + YD+SSVR + SG
Sbjct: 249 LRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTVMSG 308

Query: 310 GAPLGKELEDAVRAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAGACGTVVRNAE 369
            APLGKELED VRAKFP A L QGYGMTEAGPVL M LAFAKEPF++K+GACGTVVRNAE
Sbjct: 309 AAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAE 368

Query: 370 MKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFI-DDD 429
           MKIVDP+TG SLP N +GEICIRGDQIMKGYLND E+T RTIDKEGWL+TGDIG+I DDD
Sbjct: 369 MKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDDD 428

Query: 430 ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMADEEAGEVPVAFVVKESD 489
           ELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M DE+AGEVPVAFVV+ + 
Sbjct: 429 ELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNG 488

Query: 490 GTITEEEVKHFITKQVVFYKRLKSVYFVNAIPKAPSGKILRKELRTKLASGAYN 542
            TITE+EVK FI+KQV+FYKR+K V+FV+AIPK+PSGKILRK+LR KLA+G  N
Sbjct: 489 STITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542

BLAST of Bhi09G000462 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 5.0e-224
Identity = 379/540 (70.19%), Postives = 448/540 (82.96%), Query Frame = 0

Query: 4   ESNQTNEFIFRSKLPDIHIPNHLPLHDYIFQNLPKFASRPCLINGATGDVYSFHDVQLTA 63
           E+ Q+ + IFRSKLPDI+IP HLPLH Y F+N+ +F+SRPCLINGA   +Y++ +V+LT 
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKA 123
           R+VA GL+ LGI++ D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 ANAKLIVTMACFYDRVKDLA-ENGVKIVCVDFAVEGCLHFSVLSGADEAHAPAVDFSPDD 183
           ++AK+I+T +CF  +VKD A EN VK++C+D A EGCLHFS L+ +DE   P V   PDD
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDD 187

Query: 184 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYSLN 243
           VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY   +DV++CVLP FHIYSLN
Sbjct: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 247

Query: 244 SILLCGIRAGAAIMIMQKFDIVSLFQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVSSV 303
           SILLCG+R GAAI+IMQKFDI    +LI+KY++SI P VPPI LAIAKS   + YD+SSV
Sbjct: 248 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 307

Query: 304 RVLKSGGAPLGKELEDAVRAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAGACGT 363
           R + SG APLGKELEDAVR KFP A L QGYGMTEAGPVL M LAFAKEPF +K+GACGT
Sbjct: 308 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 367

Query: 364 VVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIG 423
           VVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND E+T RTIDKEGWLHTGDIG
Sbjct: 368 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 427

Query: 424 FID-DDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMADEEAGEVPVAF 483
           FID DDELFIVDRLKELIK+K FQVAPAE+EALL+ HP +SDAAV+ M DE+AGEVPVAF
Sbjct: 428 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 487

Query: 484 VVKESDGTITEEEVKHFITKQVVFYKRLKSVYFVNAIPKAPSGKILRKELRTKLASGAYN 542
           VV+ +   ITE+EVK FI+KQV+FYKR+K V+FV  +PK+PSGKILRK+LR +LA+G  N
Sbjct: 488 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

BLAST of Bhi09G000462 vs. ExPASy Swiss-Prot
Match: O24540 (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 1.9e-223
Identity = 385/540 (71.30%), Postives = 451/540 (83.52%), Query Frame = 0

Query: 7   QTNEFIFRSKLPDIHIPNHLPLHDYIFQNLPKFASRPCLINGATGDVYSFHDVQLTARRV 66
           Q  + IFRSKLPDI+IP +LPLH Y F+N+ KF+SRPCLINGAT +++++ DV+L +RRV
Sbjct: 10  QKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELISRRV 69

Query: 67  AAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANA 126
            +GL  LGIK+GD +M LLPNSPEFVF FLGAS+ G+I T ANPF+T+ E+ KQAKA+NA
Sbjct: 70  GSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQAKASNA 129

Query: 127 KLIVTMACFYDRVKDLA-ENGVKIVCVDFA-----VEGCLHFSVLSGADEAHAPAVDFSP 186
           KLI+T  C+ D+VKD A ENGVKI+ +D           LHFS L+GADE   P V+ SP
Sbjct: 130 KLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADENEMPKVEISP 189

Query: 187 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 246
           D VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY   DDV+LCVLP FHIYS
Sbjct: 190 DGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLPLFHIYS 249

Query: 247 LNSILLCGIRAGAAIMIMQKFDIVSLFQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 306
           LNS+LLCG+RAG+ I+IMQKF+IV   +LI+KY+++I P VPPI LAIAKS+  + YD+S
Sbjct: 250 LNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVVDNYDLS 309

Query: 307 SVRVLKSGGAPLGKELEDAVRAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 366
           SVR + SG APLGKELEDAVRAKFP A L QGYGMTEAGPVL M LAFAKEPF +K+GAC
Sbjct: 310 SVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGAC 369

Query: 367 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 426
           GTVVRNAEMKIVDPETG+SLP N  GEICIRGDQIMKGYLND E+T RTIDKEGWLHTGD
Sbjct: 370 GTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKEGWLHTGD 429

Query: 427 IGFI-DDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMADEEAGEVPV 486
           IG+I DDDELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M DE AGEVPV
Sbjct: 430 IGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEAAGEVPV 489

Query: 487 AFVVKESDGTITEEEVKHFITKQVVFYKRLKSVYFVNAIPKAPSGKILRKELRTKLASGA 540
           AFVVK +   ITE+E+K FI+KQV+FYKR+  V+FV AIPKAPSGKILRK+LR +LA+ A
Sbjct: 490 AFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRARLAAAA 549

BLAST of Bhi09G000462 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 4.2e-223
Identity = 380/543 (69.98%), Postives = 450/543 (82.87%), Query Frame = 0

Query: 1   MALESNQTNEFIFRSKLPDIHIPNHLPLHDYIFQNLPKFASRPCLINGATGDVYSFHDVQ 60
           M  E+NQ  + IFRSKLPDI+IP HLPLH Y F+N+ +F+SRPCLINGA   +Y++ DV+
Sbjct: 3   METETNQ-GDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVE 62

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+R+VAAGL+ LGI++ D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 63  LTSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVKIVCVDFAVEGCLHFSVLSGADEAHAPAVDFS 180
           AKA+NAKLI+T ACF ++VKD A +N + ++C+D A EGC+HFS L+ ADE   P V   
Sbjct: 123 AKASNAKLIITQACFVNKVKDYAFDNNLNVICIDSAPEGCIHFSELTQADEHDIPDVKIQ 182

Query: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY   +DV++CVLP FHIY
Sbjct: 183 SDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGIRAGAAIMIMQKFDIVSLFQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDV 300
           SLNS+LLCG+R GAAI+IMQKFDIV   +LIEKY+++I P VPPI LAIAKS   + YD+
Sbjct: 243 SLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYDL 302

Query: 301 SSVRVLKSGGAPLGKELEDAVRAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAGA 360
           SSVR + SG APLGKELEDAVR KFP A L QGYGMTEAGPVL M LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND  +T RTIDKEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHTG 422

Query: 421 DIGFID-DDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMADEEAGEVP 480
           DIG+ID DDELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M DE+AGEVP
Sbjct: 423 DIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 482

Query: 481 VAFVVKESDGTITEEEVKHFITKQVVFYKRLKSVYFVNAIPKAPSGKILRKELRTKLASG 540
           VAFVV+ +   ITE+EVK F++KQV+FYKR+K V+FV  +PK+PSGKILRK+LR +LA+G
Sbjct: 483 VAFVVRSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 542
             N
Sbjct: 543 VPN 544

BLAST of Bhi09G000462 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 1.0e-221
Identity = 378/543 (69.61%), Postives = 447/543 (82.32%), Query Frame = 0

Query: 1   MALESNQTNEFIFRSKLPDIHIPNHLPLHDYIFQNLPKFASRPCLINGATGDVYSFHDVQ 60
           M +E+ Q+ + IFRSKLPDI+IP HLPLH Y F+NL +F SRPCLI+GA   +Y++ +V+
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+R+VA GL+ LGI++ D +M LLPN PEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVKIVCVDFAVEGCLHFSVLSGADEAHAPAVDFS 180
           AKA++AK+++T ACF  +VKD A EN +K++CVD A EGC+HFS L  +DE   P V   
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIY 240
           PDDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY   DDV++CVLP FHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGIRAGAAIMIMQKFDIVSLFQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDV 300
           SLNS+LLC +R GAAI+IMQKFDI    +LI K++++I P VPPI LAIAKS     YD+
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 302

Query: 301 SSVRVLKSGGAPLGKELEDAVRAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAGA 360
           SSVR + SG APLGKELEDAVRAKFP A L QGYGMTEAGPVL M LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND E+T RTI+KEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DIGFI-DDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMADEEAGEVP 480
           DIGFI DDDELFIVDRLKELIK+K FQVAPAELEALLI HP +SDAAV+ M DE+AGEVP
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFVVKESDGTITEEEVKHFITKQVVFYKRLKSVYFVNAIPKAPSGKILRKELRTKLASG 540
           VAFVV+ +  TITE+EVK FI+KQV+FYKR+K V+FV  +PK+PSGKILRK+LR +LA+G
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 542
             N
Sbjct: 543 ISN 545

BLAST of Bhi09G000462 vs. ExPASy TrEMBL
Match: A0A0A0K8H9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G430240 PE=4 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 5.8e-284
Identity = 499/542 (92.07%), Postives = 518/542 (95.57%), Query Frame = 0

Query: 1   MALESNQTNEFIFRSKLPDIHIPNHLPLHDYIFQNLPKFASRPCLINGATGDVYSFHDVQ 60
           MA ESN+TNEFIFRSKLPDIHIPNHLPLHDY+FQNL KFASRPCLINGATGDVY++HDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADEAHAPAVDFSP 180
           AKAANAKLIVTMACFYDRVKDLAENGV+IVCVDFAVEGCLHFSVLSGADE+ AP VDFS 
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY  +DVILCVLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGIRAGAAIMIMQKFDIVSLFQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 300
           LNSILLCG+R GAAIMIMQKFDIV+L QLIEK+RISIMPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFP AIL QGYGMTEAGPVL+MSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLPANS+GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDD-ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMADEEAGEVPV 480
           IGF+DDD ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGM D EAGEVPV
Sbjct: 421 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKESDGTITEEEVKHFITKQVVFYKRLKSVYFVNAIPKAPSGKILRKELRTKLASGA 540
           AFV+K + G I+EEEVK FI KQVVFYKRLK V+FVNAIPKAPSGKILRKELR KLASGA
Sbjct: 481 AFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 542
           YN
Sbjct: 541 YN 542

BLAST of Bhi09G000462 vs. ExPASy TrEMBL
Match: A0A5A7US61 (4-coumarate--CoA ligase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001380 PE=4 SV=1)

HSP 1 Score: 985.3 bits (2546), Expect = 9.9e-284
Identity = 497/542 (91.70%), Postives = 515/542 (95.02%), Query Frame = 0

Query: 1   MALESNQTNEFIFRSKLPDIHIPNHLPLHDYIFQNLPKFASRPCLINGATGDVYSFHDVQ 60
           MA ESNQT EFIFRSKLPDIHIPNHLPLH Y+FQN+PKFASRPCLINGATGDVY++HDVQ
Sbjct: 1   MAFESNQTQEFIFRSKLPDIHIPNHLPLHQYVFQNMPKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADEAHAPAVDFSP 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADE+ AP VDFS 
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESLAPVVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYY  DDVIL VLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGIRAGAAIMIMQKFDIVSLFQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 300
           LNSILLCG+R GA I+IMQKFDIVSL QLIEK++ISIMPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRVGATILIMQKFDIVSLLQLIEKHKISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFP A+L QGYGMTEAGPVL+MSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLPANS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFI-DDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMADEEAGEVPV 480
           IGF+ DDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGM D EAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKESDGTITEEEVKHFITKQVVFYKRLKSVYFVNAIPKAPSGKILRKELRTKLASGA 540
           AFVVK + G ITEEEVK FITKQVVFYKRLK V+FVNAIPKAPSGKILRKELR KLA GA
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLALGA 540

Query: 541 YN 542
           YN
Sbjct: 541 YN 542

BLAST of Bhi09G000462 vs. ExPASy TrEMBL
Match: A0A0A0K658 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G430250 PE=4 SV=1)

HSP 1 Score: 984.9 bits (2545), Expect = 1.3e-283
Identity = 497/542 (91.70%), Postives = 517/542 (95.39%), Query Frame = 0

Query: 1   MALESNQTNEFIFRSKLPDIHIPNHLPLHDYIFQNLPKFASRPCLINGATGDVYSFHDVQ 60
           MA ESN+TNEFIFRSKLPDIHIPNHLPLHDY+FQNL KFASRPCLINGATGD Y++HDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADEAHAPAVDFSP 180
           AKAANAKLIVTMACFYDRVKDLAENGV++VCVDFAVEGCLHFSVLSGADE+ AP VDFS 
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYY  DDVIL VLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGIRAGAAIMIMQKFDIVSLFQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 300
           LNSILLCG+R GAAI+IMQKFDIVSL QLIEK+RISIMPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFP A+L QGYGMTEAGPVL+MSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLPANS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFI-DDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMADEEAGEVPV 480
           IGF+ DDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGM D EAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKESDGTITEEEVKHFITKQVVFYKRLKSVYFVNAIPKAPSGKILRKELRTKLASGA 540
           AFVVK + G ITEEEVK FITKQVVFYKRLK V+FVNAIPKAPSGKILRKELR KLASGA
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 542
           YN
Sbjct: 541 YN 542

BLAST of Bhi09G000462 vs. ExPASy TrEMBL
Match: A0A1S3CE71 (4-coumarate--CoA ligase 1-like OS=Cucumis melo OX=3656 GN=LOC103499867 PE=4 SV=1)

HSP 1 Score: 984.2 bits (2543), Expect = 2.2e-283
Identity = 496/542 (91.51%), Postives = 515/542 (95.02%), Query Frame = 0

Query: 1   MALESNQTNEFIFRSKLPDIHIPNHLPLHDYIFQNLPKFASRPCLINGATGDVYSFHDVQ 60
           MA ESNQT EFIFRSKLPDIHIPNHLPLH Y+FQN+PKFA+RPCLINGATGDVY++HDVQ
Sbjct: 1   MAFESNQTQEFIFRSKLPDIHIPNHLPLHQYVFQNMPKFATRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADEAHAPAVDFSP 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADE+ AP VDFS 
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESLAPVVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYY  DDVIL VLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGIRAGAAIMIMQKFDIVSLFQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 300
           LNSILLCG+R GA I+IMQKFDIVSL QLIEK++ISIMPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRVGATILIMQKFDIVSLLQLIEKHKISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFP A+L QGYGMTEAGPVL+MSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLPANS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFI-DDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMADEEAGEVPV 480
           IGF+ DDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGM D EAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKESDGTITEEEVKHFITKQVVFYKRLKSVYFVNAIPKAPSGKILRKELRTKLASGA 540
           AFVVK + G ITEEEVK FITKQVVFYKRLK V+FVNAIPKAPSGKILRKELR KLA GA
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLALGA 540

Query: 541 YN 542
           YN
Sbjct: 541 YN 542

BLAST of Bhi09G000462 vs. ExPASy TrEMBL
Match: A0A6J1JN39 (4-coumarate--CoA ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488383 PE=4 SV=1)

HSP 1 Score: 981.1 bits (2535), Expect = 1.9e-282
Identity = 489/542 (90.22%), Postives = 519/542 (95.76%), Query Frame = 0

Query: 1   MALESNQTNEFIFRSKLPDIHIPNHLPLHDYIFQNLPKFASRPCLINGATGDVYSFHDVQ 60
           MA+ESNQT+EFIFRSKLPDIHIPNHLPLH+Y+FQN+ KF SRPCLINGATGD+YS+HDVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDIYSYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           L +RRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASY GA+MTAANPFYTAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADEAHAPAVDFSP 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLH+SVLSGADE+H P V+F+ 
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPPVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYY G DVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGIRAGAAIMIMQKFDIVSLFQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 300
           LNSILLCG+RAGAAI+IMQKF+IVSL +LIEK+++SIMPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFP A+L QGYGMTEAGPVLTMSLAFAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFAVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFI-DDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMADEEAGEVPV 480
           IGFI DDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIGM DE+AGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKESDGTITEEEVKHFITKQVVFYKRLKSVYFVNAIPKAPSGKILRKELRTKLASGA 540
           AFVVK + G ITEE+VK FI+KQVVFYKRLK V+FVNAIPKAPSGKILRKELR KLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 542
           +N
Sbjct: 541 FN 542