Bhi09G000277 (gene) Wax gourd (B227) v1

Overview


Name	Bhi09G000277
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	vacuolar protein sorting-associated protein 2 homolog 1-like
Location	chr9: 7034539 .. 7038227 (-)
RNA-Seq Expression	Bhi09G000277
Synteny	Bhi09G000277

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCAGAAGAAACGGTGGCTCCTCCATGGCGAGAGACCACCACCGTGGCCGGTGACATCGTCGACGGACTGTGAACAGGGTGAGAGTTGCAGAGTTACGAAAGGTGAAGAGAAGAAGGAATAAACTCATCGGGATCGGAAATTGCAATTATGAGCTTCCTCTTTGGCAGCCGAAAAACTCCCGCAGGTTCATAACGTTCTTACCTTCTCTAATTATCCATATTCTGGAGCTTTGTTCTTAATAAAATCGTGCTTTCTTGTGATGAATTTCGAAGTCTCGAGTCTAGGCGTATGAACGTCTATTTAATGTGGATGAAATTCTGCCTTCTTACCTTTTCAATTATGCAAATCTATGGAGATCAGTCCTGTAAACGTCATTTTGATGTGGATGAAATTCCTTTGACAGTGATTTCATTACATACTCGTGATGTCGAGGAATGAAAATTTTCCCATGCGTTATAATTTGTATGAGTAAATTTGTGCGTTCTACTTCTGTTGCAATAGACGGCTTGAGATTGTAATCTGTTTTGGTTTTCTGCGCAGAGCTTCTGCGGGAGAACAAGAGGATGCTCGATAAGTCCATTAGGGAAATTGAACGGGAAAGACAAGGCTTACAGTCTCAGGAAAAGAAGTTGATTGTCGAGATAAAGAAAAATGCTAAACAAGGACAGATGGTGAGTTAAGCAATTTAGAACTTGTTCTTTAACATCGACACATTTTCAGTGACTACGTTTCTGTAGCTCTATAGTGGAAATGTGTTTCTACATCTGATTCGAAATCTAGTCTTCCTTTCTTTCGTTTCTATAGGAAAGGCGATAGAAACTAGGTTGGAGCCATCAATTCCGCACAATTTCCTTGATTGATTCCTTTTATCTTTTTCAGGGAGCTGTTAAAATAATGGCCAAAGATCTTATCAGGACACGGCATCAGATTGAAAAATTTTATAAACTAAAATCACAGCTCCAGGGCGTGGCTTTGAGAATTCAGGTACTGTATGTGAACCTTTCTCCGAAACTAATGTTACCTGTCATCTTTCATTTTTCATGAGAGCAAGTACAATTGCTACTGAGAAATTTTTGTACAAATTTTTGGATTATAATTACTGATTTGAAGTGCTAGGACGCAGATGTAGTTATTCCTGATAGATGACCTCTAAAAGCCTAAAATGGAAGTTTAAGGTTATAACTATCTAAAGCTTGTTAAGTTTCATTATATATGCATTCTATTGGTTTAAATTCTGATTGAGGTGCACTTATACCTGTGCTAAGAAAAGAAACATTTTACAAACCATACAGTCTTCTTCCGACATAATTAAGAACAAATAAGAAATGCTTTAGTATTAGTTTAATTTCACTTGCATCCTCTAATTTTGTTGTGTTGTGAACGGAAGATTTGAACAAAATTGGGATTTTGAGGCAATATTATTTTCTTGCTTCATGTTGATGTGCAAATTGGAATTTGTAGTTTATCCAAATCACTTTATAGAAAAATTGACTTGATTCCATTAAATCTTATCTGCATTTTTAGTGTTTGTTTCTAAATTGATTTTTAATTAAAAAAAATTGGGAAAACTATCTCTTTAGTGTTTTTAAGTGTTTTGATTTCAGAAACTCTATTGGGAAGGTTGTTTTTAGTATGCCTGCCTCTTGTTTACTTTTTTGGTACATTTTGTGGTGTATCTAATCTGTCAGATTCTGGATCACTATACATTGAGATACACTATGAATAATTTTAACCTTTTCTTATTGAATTTTGTAAATATCTTTTTTTAATAAACATCATCCTCAAATGAACTCTTACTTTCTTTTTGAGACCATAAACATATAAACTCATCTTTTTTAATCGACACATAAAGTTTATACAGTTGTGGTGATTCAAATTTTGCTCCAATTGTTTGCTTCCTGCAGACGCTGAAATCAACTCAAGCTATGGGAGAAGCCATGAAAGGCGTTACCAAGGCAATGGGACAGATGAATAGGCAGATGAACTTGCCGGCATTACAGAAGATAATGCAAGAGTTTGAGAGACAAAATGAGAGGATGGAAATGACATCTGAAGTAATGGCAGACGCTCTCGATGATGCTTTAGAAGGAGATGAAGAAGAGGAGGAGACAGACGAGCTTGTAAATCAGGTGCTTGATGAGATTGGAATTGATATCAATTCAGAGGTAAATGTGCTTGCACACTTAACATTTTTTTATTTCATAATGGTTCATATATTGTCGTGGGAAACAGTAATTTAAACGCGATCATGATTTCTTTTTGTTATCTTCTACTTGCAGCTTGTGAATGCACCATCAGCTTCTGTGTCAGTATCAACTGCAAATAATAGGGTTGCCCAAGCCGAAGCCGAAGATACTGGAATTGACAGTGATTTACAAGCTAGACTTGATAACTTGAGGAAAATGTAGGTTTCAATTATGAGAAATTTATAGCACTCATATAGTATACATAATAAATAATGTATTGTGTTTATATTTGAACTCTATTCAGCCAAGTTTGTATTTGAATAATACACTGACAAGTATGATCTCTTGTTTGGAGAAGTAAGACGATGAATTTAGCACTTGTGATTAGGTAATTCTCTATTTTACAAAGGGTTTGAATTGTTAGTATATGCTAGCACGAGCTTAACCATTCTATTTCTTTGTCAAGTTGTTCTAGAACCAGAAGCCTACATGCTTTCTGAGTGCATATCGGGATCTTCTCGTTCCGATATGCAGTCAAATTATTAAACCATGTTTTCAGGCCATGCCTCCCGCCTACTTCCATGAGGAGAAAGCTCAATCTGGAGATTGTTATTAATGGCTACTACCTGAGGATTCTCCACCTCTGACGTCAACTCCCCATTAATACAAGCTACATGGTCGATTGGAGTCCGGCAGAGCGGGCAGTTTGAATGTGAAGACAGCCATACGTCGATACATGAAGCATGAAAGACATGGTTGCAGGTGGGAAGCTGCCGTATCCATTCCCCATCTTCATAAGGCGACAAGCACAAAACACACTCACTTTCAGTGGTTTCTTCACTTTCATTTTCTTTCTTGTACATGATAGTTGGGATCTTCAGGACATTTGAGTCTTCGTGTCTAATATTCTTAACTGACTCAGTCGTCTCAGGAGAACGGCAACAGCAATTAAACCATCCTAGTATTGTAAGATTGTAGAATGAGAGGGCTATAAACAGCGAGCAAATAATGGCAACACCAGAGCAGATGAAGAAGACGGACATAGCAAATGGTAAGGAAGG

mRNA sequence

CCAGAAGAAACGGTGGCTCCTCCATGGCGAGAGACCACCACCGTGGCCGGTGACATCGTCGACGGACTGTGAACAGGGTGAGAGTTGCAGAGTTACGAAAGGTGAAGAGAAGAAGGAATAAACTCATCGGGATCGGAAATTGCAATTATGAGCTTCCTCTTTGGCAGCCGAAAAACTCCCGCAGAGCTTCTGCGGGAGAACAAGAGGATGCTCGATAAGTCCATTAGGGAAATTGAACGGGAAAGACAAGGCTTACAGTCTCAGGAAAAGAAGTTGATTGTCGAGATAAAGAAAAATGCTAAACAAGGACAGATGGGAGCTGTTAAAATAATGGCCAAAGATCTTATCAGGACACGGCATCAGATTGAAAAATTTTATAAACTAAAATCACAGCTCCAGGGCGTGGCTTTGAGAATTCAGACGCTGAAATCAACTCAAGCTATGGGAGAAGCCATGAAAGGCGTTACCAAGGCAATGGGACAGATGAATAGGCAGATGAACTTGCCGGCATTACAGAAGATAATGCAAGAGTTTGAGAGACAAAATGAGAGGATGGAAATGACATCTGAAGTAATGGCAGACGCTCTCGATGATGCTTTAGAAGGAGATGAAGAAGAGGAGGAGACAGACGAGCTTGTAAATCAGGTGCTTGATGAGATTGGAATTGATATCAATTCAGAGCTTGTGAATGCACCATCAGCTTCTGTGTCAGTATCAACTGCAAATAATAGGGTTGCCCAAGCCGAAGCCGAAGATACTGGAATTGACAGTGATTTACAAGCTAGACTTGATAACTTGAGGAAAATGTAGGTTTCAATTATGAGAAATTTATAGCACTCATATAGTATACATAATAAATAATGTATTGTGTTTATATTTGAACTCTATTCAGCCAAGTTTGTATTTGAATAATACACTGACAAGTATGATCTCTTGTTTGGAGAAGTAAGACGATGAATTTAGCACTTGTGATTAGGTAATTCTCTATTTTACAAAGGGTTTGAATTGTTAGTATATGCTAGCACGAGCTTAACCATTCTATTTCTTTGTCAAGTTGTTCTAGAACCAGAAGCCTACATGCTTTCTGAGTGCATATCGGGATCTTCTCGTTCCGATATGCAGTCAAATTATTAAACCATGTTTTCAGGCCATGCCTCCCGCCTACTTCCATGAGGAGAAAGCTCAATCTGGAGATTGTTATTAATGGCTACTACCTGAGGATTCTCCACCTCTGACGTCAACTCCCCATTAATACAAGCTACATGGTCGATTGGAGTCCGGCAGAGCGGGCAGTTTGAATGTGAAGACAGCCATACGTCGATACATGAAGCATGAAAGACATGGTTGCAGGTGGGAAGCTGCCTCGTCTCAGGAGAACGGCAACAGCAATTAAACCATCCTAGTATTGTAAGATTGTAGAATGAGAGGGCTATAAACAGCGAGCAAATAATGGCAACACCAGAGCAGATGAAGAAGACGGACATAGCAAATGGTAAGGAAGG

Coding sequence (CDS)

ATGAGCTTCCTCTTTGGCAGCCGAAAAACTCCCGCAGAGCTTCTGCGGGAGAACAAGAGGATGCTCGATAAGTCCATTAGGGAAATTGAACGGGAAAGACAAGGCTTACAGTCTCAGGAAAAGAAGTTGATTGTCGAGATAAAGAAAAATGCTAAACAAGGACAGATGGGAGCTGTTAAAATAATGGCCAAAGATCTTATCAGGACACGGCATCAGATTGAAAAATTTTATAAACTAAAATCACAGCTCCAGGGCGTGGCTTTGAGAATTCAGACGCTGAAATCAACTCAAGCTATGGGAGAAGCCATGAAAGGCGTTACCAAGGCAATGGGACAGATGAATAGGCAGATGAACTTGCCGGCATTACAGAAGATAATGCAAGAGTTTGAGAGACAAAATGAGAGGATGGAAATGACATCTGAAGTAATGGCAGACGCTCTCGATGATGCTTTAGAAGGAGATGAAGAAGAGGAGGAGACAGACGAGCTTGTAAATCAGGTGCTTGATGAGATTGGAATTGATATCAATTCAGAGCTTGTGAATGCACCATCAGCTTCTGTGTCAGTATCAACTGCAAATAATAGGGTTGCCCAAGCCGAAGCCGAAGATACTGGAATTGACAGTGATTTACAAGCTAGACTTGATAACTTGAGGAAAATGTAG

Protein sequence

MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVKIMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM

Homology

[Close]

BLAST of Bhi09G000277 vs. TAIR 10
Match: AT2G06530.1 (SNF7 family protein )

HSP 1 Score: 343.6 bits (880), Expect = 1.2e-94
Identity = 193/224 (86.16%), Postives = 208/224 (92.86%), Query Frame = 0


Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           M+ +FG RKTPAELLRENKRMLDKSIREIERERQGLQ+QEKKLI EIKK AKQGQMGAVK
Sbjct: 2   MNSIFGKRKTPAELLRENKRMLDKSIREIERERQGLQTQEKKLINEIKKTAKQGQMGAVK 61

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           +MAKDLIRTRHQIEKFYKLKSQLQGV+LRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP
Sbjct: 62  VMAKDLIRTRHQIEKFYKLKSQLQGVSLRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 121

Query: 121 ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180
           +LQKIMQEFERQNE+MEM SEVM DA+DDALEGDEEEEET++LV+QVLDEIGIDIN ELV
Sbjct: 122 SLQKIMQEFERQNEKMEMVSEVMGDAIDDALEGDEEEEETEDLVSQVLDEIGIDINQELV 181

Query: 181 NAPSASVSVSTANNRVAQAE---AEDT-GIDSDLQARLDNLRKM 221
           NAPS +V+V  A N+V QAE   AED+ GIDSDLQARLDNLRKM
Sbjct: 182 NAPSGAVAVPAAKNKVVQAEATGAEDSGGIDSDLQARLDNLRKM 225

[Close]

BLAST of Bhi09G000277 vs. TAIR 10
Match: AT1G03950.1 (vacuolar protein sorting-associated protein 2.3 )

HSP 1 Score: 155.2 bits (391), Expect = 6.0e-38
Identity = 93/215 (43.26%), Postives = 143/215 (66.51%), Query Frame = 0


Query: 4   LFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVKIMA 63
           +F  +  P E+LRE+KR + ++ R IE+E   LQS+EKKL++EIK+ AK G  GA KI+A
Sbjct: 3   IFTKKPNPREVLRESKREMTQATRGIEKEIGSLQSEEKKLVLEIKRTAKSGNEGATKILA 62

Query: 64  KDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLPALQ 123
           + LIR R QI      ++Q++G+A   Q + +  ++   M+G TKAM  M++ M+     
Sbjct: 63  RQLIRLRQQIANLQGSRAQMRGIATHTQAMHAHTSVAAGMQGATKAMAAMSKNMDPAKQA 122

Query: 124 KIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELVNAP 183
           K+M+EF++Q+ +M+MT+E+M+D++DDAL+ DE E+ET++L NQVLDEIGIDI S+L +AP
Sbjct: 123 KVMREFQKQSAQMDMTTEMMSDSIDDALDNDEAEDETEDLTNQVLDEIGIDIASQLSSAP 182

Query: 184 SASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR 219
              +    A       +   +GID +L+ RL  LR
Sbjct: 183 KGKIGGKKAE------DVGSSGID-ELEKRLAALR 210

[Close]

BLAST of Bhi09G000277 vs. TAIR 10
Match: AT5G44560.1 (SNF7 family protein )

HSP 1 Score: 144.1 bits (362), Expect = 1.4e-34
Identity = 87/221 (39.37%), Postives = 141/221 (63.80%), Query Frame = 0


Query: 4   LFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVKIMA 63
           +F  + TP + LR +KR +  + R IERE   LQ +EK+L+ EIKK AK G   A KI+A
Sbjct: 3   IFKKKTTPKDALRTSKREMAVATRGIEREITSLQLEEKRLVAEIKKTAKTGNEAATKILA 62

Query: 64  KDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLPALQ 123
           + L+R R QI      ++Q++GV    Q L ++ ++   MKG TKAM  MN+QM      
Sbjct: 63  RQLVRLRQQITNLQGSRAQIRGVTTHTQALYASTSISSGMKGATKAMVAMNKQMAPTKQA 122

Query: 124 KIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELVNAP 183
           K++++F++Q+ +++MT E+M++A+D+ L+ DE EEET++L NQVLDEIG+ + S+L +AP
Sbjct: 123 KVIKDFQKQSAQLDMTIEMMSEAIDETLDKDEAEEETEDLTNQVLDEIGVGVASQLSSAP 182

Query: 184 SASVSVST----ANNRVAQAEAEDTGIDSDLQARLDNLRKM 221
              ++  T    A+       +E + +D +L+ RL +LR++
Sbjct: 183 KGRIATKTAAPPASTAATNKNSESSEVD-ELEKRLASLRRI 222

[Close]

BLAST of Bhi09G000277 vs. TAIR 10
Match: AT5G44560.2 (SNF7 family protein )

HSP 1 Score: 131.7 bits (330), Expect = 7.1e-31
Identity = 80/198 (40.40%), Postives = 128/198 (64.65%), Query Frame = 0


Query: 27  REIERERQGLQSQEKKLIVEIKKNAKQGQMGAVKIMAKDLIRTRHQIEKFYKLKSQLQGV 86
           R IERE   LQ +EK+L+ EIKK AK G   A KI+A+ L+R R QI      ++Q++GV
Sbjct: 6   RGIEREITSLQLEEKRLVAEIKKTAKTGNEAATKILARQLVRLRQQITNLQGSRAQIRGV 65

Query: 87  ALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADA 146
               Q L ++ ++   MKG TKAM  MN+QM      K++++F++Q+ +++MT E+M++A
Sbjct: 66  TTHTQALYASTSISSGMKGATKAMVAMNKQMAPTKQAKVIKDFQKQSAQLDMTIEMMSEA 125

Query: 147 LDDALEGDEEEEETDELVNQVLDEIGIDINSELVNAPSASVSVST----ANNRVAQAEAE 206
           +D+ L+ DE EEET++L NQVLDEIG+ + S+L +AP   ++  T    A+       +E
Sbjct: 126 IDETLDKDEAEEETEDLTNQVLDEIGVGVASQLSSAPKGRIATKTAAPPASTAATNKNSE 185

Query: 207 DTGIDSDLQARLDNLRKM 221
            + +D +L+ RL +LR++
Sbjct: 186 SSEVD-ELEKRLASLRRI 202

[Close]

BLAST of Bhi09G000277 vs. TAIR 10
Match: AT5G22950.1 (SNF7 family protein )

HSP 1 Score: 78.6 bits (192), Expect = 7.1e-15
Identity = 58/217 (26.73%), Postives = 120/217 (55.30%), Query Frame = 0


Query: 11  PAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVKIMAKDLIRTR 70
           P +LLR+ +R L +  R IER+ + +Q +E+ +   IK+ AK+  M + K +AK+++ +R
Sbjct: 14  PKQLLRDWQRKLRQECRNIERQIRDIQKEERNVQKAIKEAAKRNDMVSAKALAKEIVSSR 73

Query: 71  HQIEKFYKLKSQLQGVALRI-QTLKSTQAMGEAMKGVTKAMGQMNRQMNLPALQKIMQEF 130
             + + Y+ K+Q+  +++ + +++   + +G   K   + M  +N  M  P +   MQEF
Sbjct: 74  RTVNRLYENKAQMNSISMHLGESVAIARTVGHLSKS-AEVMKLVNNLMKAPQMAATMQEF 133

Query: 131 ERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLD--------EIGIDINSELVN 190
            ++  +  +  E + +A+D+AL+ ++ EEE DE V++VL         E+ + +  E + 
Sbjct: 134 SKEMTKAGVIEEFVNEAIDNALDSEDMEEEIDEEVDKVLTAIAGETAAELPVAVRKERIK 193

Query: 191 APSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR 219
            P+   S S     VA+   ++  ++ +++ARL  +R
Sbjct: 194 VPAQKASTSREEEAVAEGVDDEEELE-EIRARLAKVR 228

[Close]

BLAST of Bhi09G000277 vs. ExPASy Swiss-Prot
Match: Q9SKI2 (Vacuolar protein sorting-associated protein 2 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS2.1 PE=1 SV=2)

HSP 1 Score: 343.6 bits (880), Expect = 1.7e-93
Identity = 193/224 (86.16%), Postives = 208/224 (92.86%), Query Frame = 0


Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           M+ +FG RKTPAELLRENKRMLDKSIREIERERQGLQ+QEKKLI EIKK AKQGQMGAVK
Sbjct: 2   MNSIFGKRKTPAELLRENKRMLDKSIREIERERQGLQTQEKKLINEIKKTAKQGQMGAVK 61

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           +MAKDLIRTRHQIEKFYKLKSQLQGV+LRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP
Sbjct: 62  VMAKDLIRTRHQIEKFYKLKSQLQGVSLRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 121

Query: 121 ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180
           +LQKIMQEFERQNE+MEM SEVM DA+DDALEGDEEEEET++LV+QVLDEIGIDIN ELV
Sbjct: 122 SLQKIMQEFERQNEKMEMVSEVMGDAIDDALEGDEEEEETEDLVSQVLDEIGIDINQELV 181

Query: 181 NAPSASVSVSTANNRVAQAE---AEDT-GIDSDLQARLDNLRKM 221
           NAPS +V+V  A N+V QAE   AED+ GIDSDLQARLDNLRKM
Sbjct: 182 NAPSGAVAVPAAKNKVVQAEATGAEDSGGIDSDLQARLDNLRKM 225

[Close]

BLAST of Bhi09G000277 vs. ExPASy Swiss-Prot
Match: Q6IP52 (Charged multivesicular body protein 2a OS=Xenopus laevis OX=8355 GN=chmp2a PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 1.7e-58
Identity = 123/219 (56.16%), Postives = 169/219 (77.17%), Query Frame = 0


Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           M FLFG RKTP ELLR+N+R L++++RE++RERQ L+ QEKK+I +IKK AKQGQM AVK
Sbjct: 1   MEFLFGRRKTPEELLRQNQRALNRAMRELDRERQKLEQQEKKIIADIKKMAKQGQMDAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDL+RTR  ++KF  +++ +Q V+L+IQTLKS  +M +AMKGVTKAM  MNRQ+ LP
Sbjct: 61  IMAKDLVRTRRYVKKFIMMRANIQAVSLKIQTLKSNNSMAQAMKGVTKAMATMNRQLKLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180
            +QKIM EFE+Q+E M+M  E+M DA+DDA+  +++EEE+D +V+QVLDE+G+ +  EL 
Sbjct: 121 QIQKIMMEFEKQSEIMDMKEEMMNDAIDDAMGDEDDEEESDAVVSQVLDELGLTLTDELS 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRK 220
           N PS   S+S A  +  +A A     D+DL+ RL+NLR+
Sbjct: 181 NLPSTGGSLSVAGAKKGEATAALADADADLEERLNNLRR 219

[Close]

BLAST of Bhi09G000277 vs. ExPASy Swiss-Prot
Match: Q6DFS6 (Charged multivesicular body protein 2a OS=Xenopus tropicalis OX=8364 GN=chmp2a PE=2 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 5.1e-58
Identity = 122/219 (55.71%), Postives = 168/219 (76.71%), Query Frame = 0


Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           M FLFG RKTP E+LR+N+R L+K++RE++RERQ L+ QEKK+I +IKK AKQGQM AVK
Sbjct: 1   MEFLFGRRKTPEEMLRQNQRALNKAMREMDRERQKLEQQEKKIIADIKKMAKQGQMDAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDL+RTR  ++KF  +++ +Q V+L+IQTLKS  +M +AMKGVTKAM  MNRQ+ LP
Sbjct: 61  IMAKDLVRTRRYVKKFIMMRANIQAVSLKIQTLKSNNSMAQAMKGVTKAMATMNRQLKLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180
            +QKIM EFE+Q+E M+M  E+M DA+DDA+  +++EEE+D +V+QVLDE+G+ +  EL 
Sbjct: 121 QIQKIMMEFEKQSEIMDMKEEMMNDAIDDAMGDEDDEEESDAVVSQVLDELGLTLTDELS 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRK 220
           N PS   S+S A  +  +  A     D+DL+ RL+NLR+
Sbjct: 181 NLPSTGGSLSVAGAKKGEPSAALADADADLEERLNNLRR 219

[Close]

BLAST of Bhi09G000277 vs. ExPASy Swiss-Prot
Match: Q54DB1 (Charged multivesicular body protein 2a homolog 2 OS=Dictyostelium discoideum OX=44689 GN=chmp2a2 PE=3 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 2.5e-57
Identity = 123/191 (64.40%), Postives = 160/191 (83.77%), Query Frame = 0


Query: 3   FLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVKIM 62
           FLFG +KTP E+LREN+R L+KS+REI+RER  LQ+QEKK+I++IKK AKQGQM + KIM
Sbjct: 2   FLFGKQKTPKEVLRENQRNLNKSMREIDRERVALQNQEKKIILDIKKMAKQGQMNSAKIM 61

Query: 63  AKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLPAL 122
           AKDL+RTR+ I+KFY++K+QLQ V+LRIQTL+STQAM EAMKGVTKAM  MNRQMNLP  
Sbjct: 62  AKDLVRTRYHIQKFYEMKTQLQAVSLRIQTLQSTQAMAEAMKGVTKAMITMNRQMNLPQF 121

Query: 123 QKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELVNA 182
            KIM EFE+Q+++M+M  E+M D +D  +E D EEE++ E++NQVLDEIGID+ S+LV+A
Sbjct: 122 TKIMMEFEQQSDKMDMKEEMMNDTMDQVMEQDGEEEQSQEILNQVLDEIGIDLASQLVDA 181

Query: 183 PSASVSVSTAN 194
           P+ +V  S A+
Sbjct: 182 PT-TVGTSVAS 191

[Close]

BLAST of Bhi09G000277 vs. ExPASy Swiss-Prot
Match: O43633 (Charged multivesicular body protein 2a OS=Homo sapiens OX=9606 GN=CHMP2A PE=1 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 7.3e-57
Identity = 123/221 (55.66%), Postives = 167/221 (75.57%), Query Frame = 0


Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           M  LFG RKTP ELLR+N+R L++++RE++RERQ L++QEKK+I +IKK AKQGQM AV+
Sbjct: 1   MDLLFGRRKTPEELLRQNQRALNRAMRELDRERQKLETQEKKIIADIKKMAKQGQMDAVR 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDL+RTR  + KF  +++ +Q V+L+IQTLKS  +M +AMKGVTKAMG MNRQ+ LP
Sbjct: 61  IMAKDLVRTRRYVRKFVLMRANIQAVSLKIQTLKSNNSMAQAMKGVTKAMGTMNRQLKLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180
            +QKIM EFERQ E M+M  E+M DA+DDA+  +E+EEE+D +V+QVLDE+G+ +  EL 
Sbjct: 121 QIQKIMMEFERQAEIMDMKEEMMNDAIDDAMGDEEDEEESDAVVSQVLDELGLSLTDELS 180

Query: 181 NAPS--ASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRK 220
           N PS   S+SV+    +   A +     D+DL+ RL NLR+
Sbjct: 181 NLPSTGGSLSVAAGGKKAEAAASALADADADLEERLKNLRR 221

[Close]

BLAST of Bhi09G000277 vs. ExPASy TrEMBL
Match: A0A1S3CGM4 (vacuolar protein sorting-associated protein 2 homolog 1-like OS=Cucumis melo OX=3656 GN=LOC103500700 PE=4 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 2.3e-106
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0


Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK
Sbjct: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP
Sbjct: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180
           ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELV
Sbjct: 121 ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 221
           NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM
Sbjct: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220

[Close]

BLAST of Bhi09G000277 vs. ExPASy TrEMBL
Match: A0A6J1JXE8 (vacuolar protein sorting-associated protein 2 homolog 1-like OS=Cucurbita maxima OX=3661 GN=LOC111488711 PE=4 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 6.8e-106
Identity = 219/220 (99.55%), Postives = 219/220 (99.55%), Query Frame = 0


Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK
Sbjct: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP
Sbjct: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180
           ALQKIMQEFERQNERMEMTSEVMAD LDDALEGDEEEEETDELVNQVLDEIGIDINSELV
Sbjct: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 221
           NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM
Sbjct: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220

[Close]

BLAST of Bhi09G000277 vs. ExPASy TrEMBL
Match: A0A6J1GQK3 (vacuolar protein sorting-associated protein 2 homolog 1-like OS=Cucurbita moschata OX=3662 GN=LOC111456560 PE=4 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 6.8e-106
Identity = 219/220 (99.55%), Postives = 219/220 (99.55%), Query Frame = 0


Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK
Sbjct: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP
Sbjct: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180
           ALQKIMQEFERQNERMEMTSEVMAD LDDALEGDEEEEETDELVNQVLDEIGIDINSELV
Sbjct: 121 ALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 221
           NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM
Sbjct: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220

[Close]

BLAST of Bhi09G000277 vs. ExPASy TrEMBL
Match: A0A0A0K7U7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G450570 PE=4 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 2.0e-105
Identity = 218/220 (99.09%), Postives = 220/220 (100.00%), Query Frame = 0


Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK
Sbjct: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMG+AMKGVTKAMGQMNRQMNLP
Sbjct: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGDAMKGVTKAMGQMNRQMNLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180
           ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELV+QVLDEIGIDINSELV
Sbjct: 121 ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVSQVLDEIGIDINSELV 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 221
           NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM
Sbjct: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220

[Close]

BLAST of Bhi09G000277 vs. ExPASy TrEMBL
Match: A0A6J1KC98 (vacuolar protein sorting-associated protein 2 homolog 1-like OS=Cucurbita maxima OX=3661 GN=LOC111494308 PE=4 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 6.4e-104
Identity = 214/220 (97.27%), Postives = 219/220 (99.55%), Query Frame = 0


Query: 1   MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60
           MSFLFGSRKTPAE+LRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK
Sbjct: 1   MSFLFGSRKTPAEILRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVK 60

Query: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120
           IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP
Sbjct: 61  IMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLP 120

Query: 121 ALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELV 180
           ALQKIMQEFERQNE+MEMTSE+MADALDDA EGDEEEEET+ELV+QVLDEIGIDINSELV
Sbjct: 121 ALQKIMQEFERQNEKMEMTSEMMADALDDAFEGDEEEEETEELVSQVLDEIGIDINSELV 180

Query: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 221
           NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM
Sbjct: 181 NAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM 220

The following BLAST results are available for this feature:

BLAST of Bhi09G000277 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G06530.1	1.2e-94	86.16	SNF7 family protein	[more]
AT1G03950.1	6.0e-38	43.26	vacuolar protein sorting-associated protein 2.3	[more]
AT5G44560.1	1.4e-34	39.37	SNF7 family protein	[more]
AT5G44560.2	7.1e-31	40.40	SNF7 family protein	[more]
AT5G22950.1	7.1e-15	26.73	SNF7 family protein	[more]

BLAST of Bhi09G000277 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SKI2	1.7e-93	86.16	Vacuolar protein sorting-associated protein 2 homolog 1 OS=Arabidopsis thaliana ...	[more]
Q6IP52	1.7e-58	56.16	Charged multivesicular body protein 2a OS=Xenopus laevis OX=8355 GN=chmp2a PE=2 ...	[more]
Q6DFS6	5.1e-58	55.71	Charged multivesicular body protein 2a OS=Xenopus tropicalis OX=8364 GN=chmp2a P...	[more]
Q54DB1	2.5e-57	64.40	Charged multivesicular body protein 2a homolog 2 OS=Dictyostelium discoideum OX=...	[more]
O43633	7.3e-57	55.66	Charged multivesicular body protein 2a OS=Homo sapiens OX=9606 GN=CHMP2A PE=1 SV...	[more]

BLAST of Bhi09G000277 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CGM4	2.3e-106	100.00	vacuolar protein sorting-associated protein 2 homolog 1-like OS=Cucumis melo OX=...	[more]
A0A6J1JXE8	6.8e-106	99.55	vacuolar protein sorting-associated protein 2 homolog 1-like OS=Cucurbita maxima...	[more]
A0A6J1GQK3	6.8e-106	99.55	vacuolar protein sorting-associated protein 2 homolog 1-like OS=Cucurbita moscha...	[more]
A0A0A0K7U7	2.0e-105	99.09	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G450570 PE=4 SV=1	[more]
A0A6J1KC98	6.4e-104	97.27	vacuolar protein sorting-associated protein 2 homolog 1-like OS=Cucurbita maxima...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 119..139
None	No IPR available	COILS	Coil	Coil	coord: 12..46
None	No IPR available	GENE3D	6.10.140.1230		coord: 6..181 e-value: 2.6E-70 score: 237.9
None	No IPR available	PANTHER	PTHR10476	CHARGED MULTIVESICULAR BODY PROTEIN	coord: 1..220
None	No IPR available	PANTHER	PTHR10476:SF44	VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 2 HOMOLOG 1-LIKE	coord: 1..220
IPR005024	Snf7 family	PFAM	PF03357	Snf7	coord: 17..186 e-value: 2.1E-44 score: 151.1

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M000277	Bhi09M000277	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032509	endosome transport via multivesicular body sorting pathway
biological_process	GO:0045324	late endosome to vacuole transport
biological_process	GO:0015031	protein transport
biological_process	GO:0007034	vacuolar transport
cellular_component	GO:0000815	ESCRT III complex
cellular_component	GO:0005771	multivesicular body