Bhi09G000063 (gene) Wax gourd (B227) v1

Overview


Name	Bhi09G000063
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	2-oxoglutarate-dependent dioxygenase AOP1
Location	chr9: 1307629 .. 1310067 (+)
RNA-Seq Expression	Bhi09G000063
Synteny	Bhi09G000063

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAATTAAAGCACCTACCTTTTTTCTTTCTTTGATAATTATTTTTAGAACTTGTTTTTCTCTTACATAAGCCATCAAAGAAAAATGAAAAAAGGTAGCTGAGTGAGTTAGGTTGTATGAGAATGGTTAATTCTGAAGCAAAAGCCAAAGTACTTCCCATAATAGATTTGTCAAAATTAGAGGCAGTTGGCACAGAAGAATGGGGTTCAGCGTGCAAAGAGATGAGGCTTGGATTAGAAGAATTTGGTTGTTTCTCAGTGGTTTATGACAAAGTTTCTTTGGAACTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTTTTTCATCTCCCATTAGAAACTAGAACTAAGAACACTAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCACTTCTTCCTCTCTATGAATCAATGGCTATTGATAATCCAACTGAGCTACAAACAACACAGCTGAGTTTGGCCAATCTCCTGTGGCCTTCTAGAGCAAACAATCACTTCTGGTAATTAATTTAATTAACACACACTACTATTCTTGTCATCTATTTCATCCTTCTTATAGTTTTAAAAGTTTTGATTCATATGATACTAATTATTTAGTTTCAATTTGATTCTTATGAATTTGTGTCATTAAACCAAACCCACAAATAATTGATTCCTTAATGATTTGTTTAGATCTTCATATCCGAAGTGGTATGCTATAAACTTCTTGTTGTTGCTCATTGCTTTCTAGCCTATTACAATCTTTCTCTTCTCTGTTCATACATTCAAAACGCTTGCGAAAACCTTTTTCTCCAAAGCCGTTTTCTCTAAAACGAGGGTGGATGTTGCGAGAGGCACACGGTGTGCCATCGGCATTCTGTATTCGAATTGGCTGATTTGTTTGTGAGCGGAAACAAGTACCACAAAATCGCTAAGTGAAGCTTCTGAAAATGCGATTGTGACAGTTATATCACATGTGTGAGAGTGAAGATTTAATACTTACCATCTCAAATTGATAAAGTTTCTTATGGCCTGTTTGGATCACAAATATTGTTTCATAGGTATAAATATCAAATATTTGTAATTTAAATGTGTGAATTTTAAATGTTTGAGATAATTAATGTATGTATTTAGATATGCATCATCATTAATATTTAGAAGTTAATTATATGTGTTTGATTTACCAGTTTTGCATATAGAAATAAAAATTAAATAATTATTTAGTTAAAGGTTAGAGAACAAGTATGATGGTCATCAATAAAATTTAGCATGATCACTTAAAATATATGTAAATGAAGAATTTTATATTAAACGTTGATTAAATTATTGAATTTAATTACTAAAAACAATGAAATACATATAAATTAATTAGGATTTTAGAATTTTACATTAAATAGTAAGGTAACATAAAAATATTAATTTTAAATGTATTAGTGAAAGATTAATTCTAATATATTTATACTTAATAATTAATTAAAATTAATAGTAAGGATTAATTGATATTAATTTATTAATTAATTAACTTATATTTTTATTGTTCATCTACTACGTATAAAAATTTCACTTGTATGGAGGGTATTTTAAAATTCATGTCATATGATTTTTTAAGTTCTATGGATAATAATATCTTTAGTTTTAAAAAGTGAAAGGTAAAATAGATAAGGATATTAAAAAATGTCATAGCAAAACTTGTAAAATGAATAGACCTTAAAGTTGTAATACTTGTGTGTATTATGAACTACATCCATCTTTATGCAACCCACTCCAATAATATCTAAAAAGTAAAAAAAATGTTTTTTTTAAAATTTGTCATGTGGCAAATCGTGGTTGGACGAACCACCCAAGATGCATATTTTTCTTAATTATATCTTACAAAAAAAAGATTTTTTTTGAGTAATAATGTCATGCATTAAATACGAACATTTAAATTTGTATCTATTTGGTTAAACTTTAAAAGGTGTTTAATACCTTCTTCTTTTAATAGAACTATTATATATGAAATTGAATATTATAGTTAATAGAACTTATAAATAATAAAATTGACCTATTAAATATAGAGTAACTGAGAAAGATGAAACCCATACGGGTTTGAATTACCATACAGATCTCACTTTCTTGTCCATAATTCACCAGCATCTCATTTCTGGTCTTCAAATTCAATTGCTTGATGATCAATGGCTCCATGTTCAACCCTCAACCCATTGCCCCTTCATTGTCATGGCTGGCGATGTACTCATGGTAACCACACAGTCACACCAATCCTTTTTTTTTTTTTGTTTCTTCATAGCAAAATTTAGTCCTTAAACTTTTAAGACCTAATCGGATCACACCAATCCCTTTTTTTGTTTTTCCATATCAAAATTTAGTCCTTAAACTTTTTAAACCTAATCGGAATAGATCTTTTAACCATAAAACATGAGTATGGTAAAAGAAATATGTTTGTCTTGTGAAACC

mRNA sequence

AAAAAATTAAAGCACCTACCTTTTTTCTTTCTTTGATAATTATTTTTAGAACTTGTTTTTCTCTTACATAAGCCATCAAAGAAAAATGAAAAAAGGTAGCTGAGTGAGTTAGGTTGTATGAGAATGGTTAATTCTGAAGCAAAAGCCAAAGTACTTCCCATAATAGATTTGTCAAAATTAGAGGCAGTTGGCACAGAAGAATGGGGTTCAGCGTGCAAAGAGATGAGGCTTGGATTAGAAGAATTTGGTTGTTTCTCAGTGGTTTATGACAAAGTTTCTTTGGAACTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTTTTTCATCTCCCATTAGAAACTAGAACTAAGAACACTAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCACTTCTTCCTCTCTATGAATCAATGGCTATTGATAATCCAACTGAGCTACAAACAACACAGCTGAGTTTGGCCAATCTCCTGTGGCCTTCTAGAGCAAACAATCACTTCTGCATCTCATTTCTGGTCTTCAAATTCAATTGCTTGATGATCAATGGCTCCATGTTCAACCCTCAACCCATTGCCCCTTCATTGTCATGGCTGGCGATGTACTCATGGTAACCACACAGTCACACCAATCCTTTTTTTTTTTTTGTTTCTTCATAGCAAAATTTAGTCCTTAAACTTTTAAGACCTAATCGGATCACACCAATCCCTTTTTTTGTTTTTCCATATCAAAATTTAGTCCTTAAACTTTTTAAACCTAATCGGAATAGATCTTTTAACCATAAAACATGAGTATGGTAAAAGAAATATGTTTGTCTTGTGAAACC

Coding sequence (CDS)

ATGAGAATGGTTAATTCTGAAGCAAAAGCCAAAGTACTTCCCATAATAGATTTGTCAAAATTAGAGGCAGTTGGCACAGAAGAATGGGGTTCAGCGTGCAAAGAGATGAGGCTTGGATTAGAAGAATTTGGTTGTTTCTCAGTGGTTTATGACAAAGTTTCTTTGGAACTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTTTTTCATCTCCCATTAGAAACTAGAACTAAGAACACTAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCACTTCTTCCTCTCTATGAATCAATGGCTATTGATAATCCAACTGAGCTACAAACAACACAGCTGAGTTTGGCCAATCTCCTGTGGCCTTCTAGAGCAAACAATCACTTCTGCATCTCATTTCTGGTCTTCAAATTCAATTGCTTGATGATCAATGGCTCCATGTTCAACCCTCAACCCATTGCCCCTTCATTGTCATGGCTGGCGATGTACTCATGGTAA

Protein sequence

MRMVNSEAKAKVLPIIDLSKLEAVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRANNHFCISFLVFKFNCLMINGSMFNPQPIAPSLSWLAMYSW

Homology

[Close]

BLAST of Bhi09G000063 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 107.1 bits (266), Expect = 1.4e-23
Identity = 53/124 (42.74%), Postives = 76/124 (61.29%), Query Frame = 0


Query: 9   KAKVLPIIDLSKLEAV--GTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATK 68
           K   +PI+DL+  + +   T  W S  +E    LEE+GCF  VYD V+ +L +SIF A +
Sbjct: 6   KTTKVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAE 65

Query: 69  ELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRAN 128
           ELF LP ET+ KN +EKPYHGY G   ++PL+E + +D  T  +  Q    +L+WP + N
Sbjct: 66  ELFDLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQ-RFTHLMWP-QGN 125

Query: 129 NHFC 131
           + FC
Sbjct: 126 DRFC 127

[Close]

BLAST of Bhi09G000063 vs. TAIR 10
Match: AT1G52810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 97.8 bits (242), Expect = 8.5e-21
Identity = 50/128 (39.06%), Postives = 79/128 (61.72%), Query Frame = 0


Query: 13  LPIIDLSKLE-AVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHL 72
           LP+ID +  +   GT +W S   ++R  LEE+GCF  ++DKV LEL  ++F+A++E+F L
Sbjct: 12  LPVIDFTSRDLKPGTIQWDSVRADVRRALEEYGCFEALFDKVPLELRKAVFDASEEVFQL 71

Query: 73  PLETRTKNTSEKPYHGYFGGYSLLPLYESMAID---NPTELQTTQLSLANLLWPSRANNH 132
           PLET+ +  S++ Y GY G    LPL+E M +D   NP ++     +  + LWP + NN+
Sbjct: 72  PLETKKRVVSKRKYRGYVGQIPTLPLFEVMGVDFAENPDKVN----AFTHKLWP-QGNNN 131

Query: 133 FCISFLVF 137
           F  + + F
Sbjct: 132 FSEAVMSF 134

[Close]

BLAST of Bhi09G000063 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 94.7 bits (234), Expect = 7.2e-20
Identity = 49/119 (41.18%), Postives = 74/119 (62.18%), Query Frame = 0


Query: 13  LPIIDLSKLE-AVGTEEWGSACKEMRLGLEEFGCFSV-VYDKVSLELHNSIFEATKELFH 72
           +P +D S+ +   GT+ W S  + +R  LEE+GCF + + DK  L+L + +F +  +LF 
Sbjct: 5   IPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDLFD 64

Query: 73  LPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRANNHF 130
           LP +T+ KN  +KP +GY G    LPL+ES+ IDN T L+ T+ S   L+WP + N HF
Sbjct: 65  LPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATR-SFTGLMWP-QGNEHF 121

[Close]

BLAST of Bhi09G000063 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 94.4 bits (233), Expect = 9.4e-20
Identity = 45/111 (40.54%), Postives = 69/111 (62.16%), Query Frame = 0


Query: 13  LPIIDLS-KLEAVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHL 72
           LP+ID S K    G  EW     +++  L+++G F   +D++  EL  S+F A +ELF L
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFDL 71

Query: 73  PLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWP 123
           PL+T+ +N S+KP+HGY G Y ++PLYESM ID+ +++     +    LWP
Sbjct: 72  PLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDD-SDIAEKVDAFTEKLWP 121

[Close]

BLAST of Bhi09G000063 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 88.2 bits (217), Expect = 6.7e-18
Identity = 43/115 (37.39%), Postives = 65/115 (56.52%), Query Frame = 0


Query: 13  LPIIDLSKLE-AVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHL 72
           LP+ID S      G+ +W     ++   LE++GCF   +DK+S+EL+ S+FEA ++LF L
Sbjct: 14  LPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFEL 73

Query: 73  PLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRAN 127
           P+ T+ +N S KP+HGY        LYES+ I++   L+         LWP   N
Sbjct: 74  PIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVN-DFTQQLWPDHGN 123

[Close]

BLAST of Bhi09G000063 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 3.3e-17
Identity = 44/115 (38.26%), Postives = 65/115 (56.52%), Query Frame = 0


Query: 13  LPIIDLSKLE-AVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHL 72
           LP+ID S      G+ +W     ++   LE++GCF   +DK+S+EL+ S+FEA ++LF L
Sbjct: 14  LPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLFEL 73

Query: 73  PLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRAN 127
           P+ T+ +N S KP+HGY        LYES+ ID+   L+         LWP   N
Sbjct: 74  PIPTKQRNVSSKPFHGYL----CHNLYESLGIDDANVLEKVN-DFTQQLWPDHGN 123

[Close]

BLAST of Bhi09G000063 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 9.5e-17
Identity = 43/115 (37.39%), Postives = 65/115 (56.52%), Query Frame = 0


Query: 13  LPIIDLSKLE-AVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHL 72
           LP+ID S      G+ +W     ++   LE++GCF   +DK+S+EL+ S+FEA ++LF L
Sbjct: 14  LPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFEL 73

Query: 73  PLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRAN 127
           P+ T+ +N S KP+HGY        LYES+ I++   L+         LWP   N
Sbjct: 74  PIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVN-DFTQQLWPDHGN 123

[Close]

BLAST of Bhi09G000063 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 6.1e-16
Identity = 47/116 (40.52%), Postives = 63/116 (54.31%), Query Frame = 0


Query: 13  LPIIDLS-KLEAVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHL 72
           LP+I+L+ K    G+ +W     ++R  LE+FGCF   YDKVSLEL  SI +  +ELF L
Sbjct: 8   LPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELFAL 67

Query: 73  PLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRANN 128
           P+ET+ +N   KPY GY    +   L ES+ I N   L+         LWP    N
Sbjct: 68  PVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENVN-EFTQQLWPDGDGN 119

[Close]

BLAST of Bhi09G000063 vs. ExPASy Swiss-Prot
Match: Q9ZTA2 (Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.0e-15
Identity = 47/116 (40.52%), Postives = 63/116 (54.31%), Query Frame = 0


Query: 13  LPIIDLS-KLEAVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHL 72
           LP+I+L+ K    G+ +W     ++R  LE+FGCF   YDKVSLEL  SI +  +ELF L
Sbjct: 8   LPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELFAL 67

Query: 73  PLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRANN 128
           P+ET+ +N   KPY GY    +   L ES+ I N   L+         LWP    N
Sbjct: 68  PVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENIN-EFTQQLWPHGDGN 119

[Close]

BLAST of Bhi09G000063 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.4e-15
Identity = 42/115 (36.52%), Postives = 64/115 (55.65%), Query Frame = 0


Query: 13  LPIIDLSKLE-AVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHL 72
           LP+ID S      G+ +W     ++   LE++GCF   +DK+S+EL+ S+FEA ++LF L
Sbjct: 7   LPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFEL 66

Query: 73  PLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRAN 127
           P+ T+ +N S K +HGY        LYES+ I++   L+         LWP   N
Sbjct: 67  PIPTKQRNVSSKLFHGYL----CHNLYESLGINDANVLEKVN-DFTQQLWPDHGN 116

[Close]

BLAST of Bhi09G000063 vs. ExPASy TrEMBL
Match: A0A1S3CDV2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 3.2e-52
Identity = 107/137 (78.10%), Postives = 121/137 (88.32%), Query Frame = 0


Query: 7   EAKAK-VLPIIDLSKLEAVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEAT 66
           EAK+K V+P IDLSKLE VGT+EWG ACKEMR GLEEFGCFSVVYDKVSLELHNSI EAT
Sbjct: 4   EAKSKVVIPTIDLSKLE-VGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEAT 63

Query: 67  KELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRA 126
           KELFHLP+ETR KNTSEKPYHGYFGGYS LPLYESMAIDNP +L++TQ S ANL+WP+R 
Sbjct: 64  KELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQ-SFANLMWPTRD 123

Query: 127 NNHFCISFLVFKFNCLM 143
           NNHFC++  V +F+ L+
Sbjct: 124 NNHFCVT--VERFSTLV 136

[Close]

BLAST of Bhi09G000063 vs. ExPASy TrEMBL
Match: A0A0A0K8Y2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G434960 PE=4 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 5.5e-52
Identity = 106/137 (77.37%), Postives = 120/137 (87.59%), Query Frame = 0


Query: 7   EAKAK-VLPIIDLSKLEAVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEAT 66
           EAK+K V+PIIDLSKLE  GT+EWG AC+EMR GLE+FGCFSVVYDKVSLELHNSIFEAT
Sbjct: 4   EAKSKVVIPIIDLSKLEE-GTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEAT 63

Query: 67  KELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRA 126
           KELFHLP+ETR KNTSEKPYHGYFGGYS LPLYESMAIDNP +LQ TQ S ANL+WP+  
Sbjct: 64  KELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQ-SFANLMWPTTE 123

Query: 127 NNHFCISFLVFKFNCLM 143
           NNHFC++  V +F+ L+
Sbjct: 124 NNHFCVA--VERFSTLV 136

[Close]

BLAST of Bhi09G000063 vs. ExPASy TrEMBL
Match: A0A6J1JXN6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111488804 PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.4e-42
Identity = 92/141 (65.25%), Postives = 110/141 (78.01%), Query Frame = 0


Query: 4   VNSEAKAKVLPIIDLSKLEAV--GTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSI 63
           + SE +A+V P+IDLSKLE +  GTE WGS CK MR+GLEEFGCF VV+++VSLELHNS 
Sbjct: 1   MGSETQARV-PVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSF 60

Query: 64  FEATKELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLW 123
           F A  ELF LP+ETR KNTSEK YHGYFG YS LPLYESMAI+NPT+L   Q S ANL+W
Sbjct: 61  FGAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQ-SFANLMW 120

Query: 124 PSRANNHFCISFLVFKFNCLM 143
           P++ NNHF  S  + +F+ LM
Sbjct: 121 PAQGNNHF--SETIQRFSTLM 137

[Close]

BLAST of Bhi09G000063 vs. ExPASy TrEMBL
Match: A0A6J1GPR2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111456352 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 9.8e-41
Identity = 87/128 (67.97%), Postives = 100/128 (78.12%), Query Frame = 0


Query: 4   VNSEAKAKVLPIIDLSKLEAV--GTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSI 63
           + SE +A V P+IDLSKLE +  GT+ WGS CK MR+GLEEFGCF VV+++VSLELHNS 
Sbjct: 1   MGSETQAGV-PVIDLSKLEKLEAGTDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSF 60

Query: 64  FEATKELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLW 123
           F A  ELF LP+ETR KNTSEK YHGYFG YS LPLYESMAIDNPT+L   Q S ANL+W
Sbjct: 61  FGAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQ-SFANLMW 120

Query: 124 PSRANNHF 130
           P + NN F
Sbjct: 121 PDQGNNRF 126

[Close]

BLAST of Bhi09G000063 vs. ExPASy TrEMBL
Match: A0A6J1CXE5 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=3673 GN=LOC111015077 PE=3 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 9.2e-39
Identity = 85/128 (66.41%), Postives = 103/128 (80.47%), Query Frame = 0


Query: 4   VNSEAKAKVLPIIDLSKLE-AVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIF 63
           + ++AK+K+ P I+LS+ +  VG+EEW    KE+R+ LEEFGCF VVY+KVSLELHNSIF
Sbjct: 1   MGTQAKSKI-PTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIF 60

Query: 64  EATKELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWP 123
           EATKELFHLP+ETR +NTSEKPYHGYFG YS LPLYESMA+DNPT LQ  Q +  NL+WP
Sbjct: 61  EATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQ-TFTNLMWP 120

Query: 124 SRANNHFC 131
           +  N  FC
Sbjct: 121 A-GNKTFC 125

The following BLAST results are available for this feature:

BLAST of Bhi09G000063 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52800.1	1.4e-23	42.74	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52810.1	8.5e-21	39.06	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52790.1	7.2e-20	41.18	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52820.1	9.4e-20	40.54	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G03070.1	6.7e-18	37.39	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

BLAST of Bhi09G000063 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q945B6	3.3e-17	38.26	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q9ZTA3	9.5e-17	37.39	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
Q945B5	6.1e-16	40.52	2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]
Q9ZTA2	1.0e-15	40.52	Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thali...	[more]
Q944Z5	1.4e-15	36.52	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]

BLAST of Bhi09G000063 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CDV2	3.2e-52	78.10	probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX...	[more]
A0A0A0K8Y2	5.5e-52	77.37	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G434960 PE=4 SV=1	[more]
A0A6J1JXN6	1.4e-42	65.25	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1GPR2	9.8e-41	67.97	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1CXE5	9.2e-39	66.41	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=367...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 13..90 e-value: 6.4E-13 score: 49.5
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..134 e-value: 3.1E-16 score: 61.4
None	No IPR available	PANTHER	PTHR47990:SF169	2-OXOGLUTARATE-DEPENDENT DIOXYGENASE	coord: 7..135
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 7..135
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 12..124

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M000063	Bhi09M000063	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding