Bhi09G000005 (gene) Wax gourd (B227) v1

Overview


Name	Bhi09G000005
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	AB hydrolase-1 domain-containing protein
Location	chr9: 122849 .. 127230 (-)
RNA-Seq Expression	Bhi09G000005
Synteny	Bhi09G000005

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGCAATGGCCAGGGCTGTGCTCTCTCAGCCTCTCAGCCTGTCGTCGCCTTATCCACTCTATTCCCTTTCCGCCTCCGCGAAATCGTACTGCCACTTCACTGCCACCACTACAAGAATCAGACCGGCATCCGCCGCCGGAAGAATCACAACCAGCGCCAAGGGCAAAGACGAAACGGGCGACAGCGACGCCGATTCTCCCGCTTTCAATCCGTTTGGGTTCGTCACTGACAATCCGTCAAGCCGAAGTGCGATTCAACTTCCTGAGAGCCCCGCTGAGGATGGCAATGTCGGCCAGATGCTCTATGTAAGTACAATTCATTTTCCATGGCAGCAAAACTAACTTAAAAACAGTGATCGGGTAAACGTATCGGAAAATTGAATTCCCCGTTCTGATAACCAAATGGTCAATTGATCGGTGAGTTTTTTTAGAAACTTACTAATTCAAGCTACGCATGGAACCGATTTGGTCGGGCTTGTCAATTGTGATAACTCGTCTTTGACCTATTGTTCTCTGATGTCTTTTCCCGTTCTTAGGATCAATGTGTTTGATATATGTATCTCTCACTCTCGACTGTGATACCTAATCATCTTTTTTCTTGTGTTCAAGTGGACTATCAATTGAAAATGAGTAGGACATGATAACCGGGGATTTGAGCTCCAATTTTCATTCGTTGTCATTACATAGTGTTGGAAGGTTATGATAGTCCTTTTCCGTTAGATTGAAAGCATTACTGAACCTGAACTTTCATGTTTGTTTCTTTTTGGTCCTCAAACTTTCATAAGTGTCTAATATGTATCTAAACTTTCAATTTTGTGTCCACTAATTCTCTAAATTTCTAAAACTTTTCATTTTATGTCGATTCTTAATTCAATTTTGTGTGTAATAGATTTTGACCTATTTGATATATATTTTTTAAATTTAAGAATTAGACACAAAATTATAAGTTTAGAATTCAATAAGACATAAAATTCAAATTTATATCAAATAGGTCAATTAATTTTAAAAAAATTCGGATATGTTAGGGACCTAAAACACAAAATTGATAGTTTAGGGATCTATTGGACACTTTAAAGTTTCCAAACACAAATTTGATAGTTCAAGAAATCTATTGAATATTTTTGAAAGTTTAAAGATGAAGTAGACACAAGTTACCTATAAACTAACCTTATTTTTTTCATCCAATGATGGCTCTACGAAGTTTAAATTTAATAAATAGCAGGTCTATAAAAATGAGAGACTCTATTATCCAGTCCCATTATAATGTTTAAAAATTGTATACAATTCTTTGTCTAGAAACACCTTAGATCATTATGTCGGGAATCTGTTTATTTCATCAATCTTACCGCGGTGTTGTGTAGTTTTCAAGGATTTTTTATTATGGCTGAATTTGCATCACACAGTGCATTTTAATGCATCTAATATGTTTATCTTTCAATAGAGAATAGAGGACAAGGGAAAGGAATATGGCTCTTACGTAAAATCTGGGAAATTCAGATGGTTTGTTAGAGAGACTGGTATGTCATTTCAGCTATTTCCAAGTTATTTGGTTCATTGATAAGCTTTGAGAATCTTTTGATCTTGTAGGGTCTGCCAAAAGCCGGCGGGGAACTATAGTCTTCCTTCATGGCGCTCCAACACAATCTTATAGCTACCGAGTTGTTATGTCTGAGGTCTTGCTAAAATAATGTTTAAAACTGCATTAAACTTTCTTGTTATCAAGTTTCCAAAATCTCAAAACCATGAAACTCATAAACTTGTGAACCAATGGGTCGAGATAGAAAGACTTGTACAGTTCTTCAACCCCATTTTGTTTACATTTTTTTCTTACAACCGTAATCATGGCAGGACTGAGTTGGATAGTTAGATCGTCCATTTGCCCTTAAATTTCAATCTGAATCTCAATCCGACAATAATTTTAAAGTTATCTCTCAGCATTTCTTTTCTTGCTGAAACTTCACCTTTTTTATTTCACTTTCTGTTGTATTGTCTAGTTGTCTGCTCTTGGATTCCACTGCTTTGCTCCAGATTGGATAGGATTTGGTTTCAGTGACAAACCACAACCAGGATATGGCTTTAATTATACAGGTTTTTGCAACGTTTAATCACTTGTGTCATAATAATTAGTAGTTTGAATATATTCTTTTGTAGTATATGTGATATTTGAATTGACACAGAGAAAGAGTACCACGAGGAACTGGATAAACTACTTGATGTGCTCGGTGTCAACACACCCTTTAACCTAGTGATTCAGGTTGGATCTGAATTTTCCAGGAAAGAACATTTCATAGAAAGATATATAGCCAATGTGACATGCTTGTGTCATGAAGATAGACTGCTCAACTATGTACATCTATTCATATCCTACAGCATTAGTTATCTTAACACGATCTTTCTTGAAGGGCTTTCTGGTGGGATCATATGGATTGACATGGGCCTTGAAAAATCAGAGCAGGATATCAAAGATATCCATTCTAAACAGTCCACTAACGGTTTCTTCTCCTGTTCCTGGGTTATTTCAACAGCTAAGGTTCAGTATGATGATTCTAACTAATGCATTGAAAATTTAATACGAAAATGTTTGCATTTTAACTCATTTTCTTAATTCTGGACTCTGAGACTCAGAATCTACATTTAGGCATACGATGAAATAACTAATTGAGCCATATTAATTTTTTGCTTCCCTTTTAGTTCTGCTTTGCTAGCAAAGCATCCGTCAATTTTTTTTTTCTAATATAAATTCAGTAATTTTCTTAATCTGTAATAAGAATCAATCTTATGTTTCACTTCTGATCTTGGAGATACATTTTCTTTTTTTCTTTTTCAAGTTCTTGTGAATGATATGTTTCAGTTTGTTCACTCTCTGTATAGGATCCCTCTGTTTGGTGAATTTACATGCCAGAATGCAGTTATGGCTGAACGTTTTATTGAAGCAGGTAGCGCGTATGTATCTAAGATCATCTTGACACTGGATTGTTCTTCTGTTAATACTCACTGTCTTCCTGTGATGTTAATTCAAACTACAGAGTCGTTGGAATCACCATAAATCCACAGAAAAGATCAATATACAGATTATACTAAAATTAACAATTGTGAATATGACTATGAGTTCATCCAAGAGCACAATAGATTTAGCGACAATTAATACATGTATTATTTTTTAATGACACGATAACTTCCGTGAAAGTCTCTTCTTAACACAAAAATAACTGATTTACAGTTTGTTGCGTTGTAACTTGTGATATAATTAGATATTTGTTAGGCACCAAGGGCTTGGAATGTTGAAGATACCGTCTCTAAGGTTTTAGTTTCAAGCCCAAATTTAAAACTTAATAACCACTTTAGTTGTCACTTGGAGTGGACAAAGACTTCGAAGAACTAGCGGGGTGAGCCATGATCATCATGCAAGGAGTGAAGCCTCAACATCTTGGTTTTGTATTTTTAGAAGATATTAGATCATATTTTGGTTTTACCTCTTTCAAATTTTGGGATTTCGAGTTCTTGGATTATGAGATAGATATCCTACATGGGCATGTTGATGATGAAGTTTTATAAGATGAACTATTGACGTGTACATTTAGAATGTTCTGCATCTCTACTTTCAAGTATATACACAGTTCATCCAATAGTTTTCCCCTTGATTTTGGAATTTTAACCATTGTGTGCATACGTCGACAGAGTATTTTCTCTGCTTGCAGATACGTATTAAAGCTGGAAAAGGCCGATGTTTACAGACTTCCATATCTGTCTAGTGGAGGACCTGGTTTTGGTAAGTAGCAAAATTTTGTAAATTAAACTTGATCAATACAATAAATCTAAATTACCCTCATCAATTAAAGTTTATGAATTAACTTATCAATGGTAATATGCTTTTTATCAAACAAAACAAGTTAAAATAAGTTTCTCGAGACAGGAGATTAACTTCCTGGTCCATGAGAAATGATTCAAGTAATTAATCCCCCATGGAGAAATCCTGCAAACGGACAACTGTATACTTTCCTAATTCGCCTTTCTTTTCAGCTTTGCTTGAAGCTGCAAGAAAAGCTAATTTCAATGATATACTAAGTCGGGTAACTGCTGGCTTTGCTTCTGGGAGGTCAGTTTTTTCTCTTGTTACCTCCTTATTTTACTTTTAACTATTTTTCGTTGTTACAATTATGCATTTGTATGAAGTTTTTACGGTCGCTTTAGTTATCACTCACCACTACATTTTCAAAGTTGGCAACCGCCGCAAAAGAAACAAGACCACTCATTAGATTCTTCATAATAAAAGTTCATTCTGATTTTTCATCTCATTTGAGGTCGTTGAACTTTATATCAAAATAATTTCAATCTTTATTTTCAAATTTTAGTTCTACATCGTCAGGAACAAAACTTGA

mRNA sequence

ATGGAGGCAATGGCCAGGGCTGTGCTCTCTCAGCCTCTCAGCCTGTCGTCGCCTTATCCACTCTATTCCCTTTCCGCCTCCGCGAAATCGTACTGCCACTTCACTGCCACCACTACAAGAATCAGACCGGCATCCGCCGCCGGAAGAATCACAACCAGCGCCAAGGGCAAAGACGAAACGGGCGACAGCGACGCCGATTCTCCCGCTTTCAATCCGTTTGGGTTCGTCACTGACAATCCGTCAAGCCGAAGTGCGATTCAACTTCCTGAGAGCCCCGCTGAGGATGGCAATGTCGGCCAGATGCTCTATAGAATAGAGGACAAGGGAAAGGAATATGGCTCTTACGTAAAATCTGGGAAATTCAGATGGTTTGTTAGAGAGACTGGGTCTGCCAAAAGCCGGCGGGGAACTATAGTCTTCCTTCATGGCGCTCCAACACAATCTTATAGCTACCGAGTTGTTATGTCTGAGTTGTCTGCTCTTGGATTCCACTGCTTTGCTCCAGATTGGATAGGATTTGGTTTCAGTGACAAACCACAACCAGGATATGGCTTTAATTATACAGAGAAAGAGTACCACGAGGAACTGGATAAACTACTTGATGTGCTCGGTGTCAACACACCCTTTAACCTAGTGATTCAGGGCTTTCTGGTGGGATCATATGGATTGACATGGGCCTTGAAAAATCAGAGCAGGATATCAAAGATATCCATTCTAAACAGTCCACTAACGGTTTCTTCTCCTGTTCCTGGGTTATTTCAACAGCTAAGGATCCCTCTGTTTGGTGAATTTACATGCCAGAATGCAGTTATGGCTGAACGTTTTATTGAAGCAGGTAGCGCATACGTATTAAAGCTGGAAAAGGCCGATGTTTACAGACTTCCATATCTGTCTAGTGGAGGACCTGGTTTTGCTTTGCTTGAAGCTGCAAGAAAAGCTAATTTCAATGATATACTAAGTCGGGTAACTGCTGGCTTTGCTTCTGGGAGGAACAAAACTTGA

Coding sequence (CDS)

ATGGAGGCAATGGCCAGGGCTGTGCTCTCTCAGCCTCTCAGCCTGTCGTCGCCTTATCCACTCTATTCCCTTTCCGCCTCCGCGAAATCGTACTGCCACTTCACTGCCACCACTACAAGAATCAGACCGGCATCCGCCGCCGGAAGAATCACAACCAGCGCCAAGGGCAAAGACGAAACGGGCGACAGCGACGCCGATTCTCCCGCTTTCAATCCGTTTGGGTTCGTCACTGACAATCCGTCAAGCCGAAGTGCGATTCAACTTCCTGAGAGCCCCGCTGAGGATGGCAATGTCGGCCAGATGCTCTATAGAATAGAGGACAAGGGAAAGGAATATGGCTCTTACGTAAAATCTGGGAAATTCAGATGGTTTGTTAGAGAGACTGGGTCTGCCAAAAGCCGGCGGGGAACTATAGTCTTCCTTCATGGCGCTCCAACACAATCTTATAGCTACCGAGTTGTTATGTCTGAGTTGTCTGCTCTTGGATTCCACTGCTTTGCTCCAGATTGGATAGGATTTGGTTTCAGTGACAAACCACAACCAGGATATGGCTTTAATTATACAGAGAAAGAGTACCACGAGGAACTGGATAAACTACTTGATGTGCTCGGTGTCAACACACCCTTTAACCTAGTGATTCAGGGCTTTCTGGTGGGATCATATGGATTGACATGGGCCTTGAAAAATCAGAGCAGGATATCAAAGATATCCATTCTAAACAGTCCACTAACGGTTTCTTCTCCTGTTCCTGGGTTATTTCAACAGCTAAGGATCCCTCTGTTTGGTGAATTTACATGCCAGAATGCAGTTATGGCTGAACGTTTTATTGAAGCAGGTAGCGCATACGTATTAAAGCTGGAAAAGGCCGATGTTTACAGACTTCCATATCTGTCTAGTGGAGGACCTGGTTTTGCTTTGCTTGAAGCTGCAAGAAAAGCTAATTTCAATGATATACTAAGTCGGGTAACTGCTGGCTTTGCTTCTGGGAGGAACAAAACTTGA

Protein sequence

MEAMARAVLSQPLSLSSPYPLYSLSASAKSYCHFTATTTRIRPASAAGRITTSAKGKDETGDSDADSPAFNPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRIEDKGKEYGSYVKSGKFRWFVRETGSAKSRRGTIVFLHGAPTQSYSYRVVMSELSALGFHCFAPDWIGFGFSDKPQPGYGFNYTEKEYHEELDKLLDVLGVNTPFNLVIQGFLVGSYGLTWALKNQSRISKISILNSPLTVSSPVPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYVLKLEKADVYRLPYLSSGGPGFALLEAARKANFNDILSRVTAGFASGRNKT

Homology

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BLAST of Bhi09G000005 vs. TAIR 10
Match: AT1G52510.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 468.8 bits (1205), Expect = 3.7e-132
Identity = 238/321 (74.14%), Postives = 265/321 (82.55%), Query Frame = 0


Query: 13  LSLSSPYPL---YSLSASAKSYC-HFTATTTRIRPASAAGRITTSAKGKDETGDSDADSP 72
           +SL SP PL   +S + + KS     TAT ++IR  S     + +        D D D P
Sbjct: 1   MSLLSPLPLLHSFSSTVATKSTASRITATPSKIR-FSVINATSENGNSGGSKNDRDED-P 60

Query: 73  AFNPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRIEDKGKEYGSYVKSGKFRWFVRET 132
           +FNPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYR EDKGKEYGS +KSGK RWFVRET
Sbjct: 61  SFNPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRTEDKGKEYGSTIKSGKLRWFVRET 120

Query: 133 GSAKSRRGTIVFLHGAPTQSYSYRVVMSELSALGFHCFAPDWIGFGFSDKPQPGYGFNYT 192
           GS +SRRGTIVF+HGAPTQS+SYR VMSELS  GFHCFAPDWIGFGFSDKPQPGYGFNYT
Sbjct: 121 GSKESRRGTIVFVHGAPTQSFSYRTVMSELSDAGFHCFAPDWIGFGFSDKPQPGYGFNYT 180

Query: 193 EKEYHEELDKLLDVLGVNTPFNLVIQGFLVGSYGLTWALKNQSRISKISILNSPLTVSSP 252
           EKEYHE  DKLL+VL V +PF LV+QGFLVGSYGLTWALKN S++ K++ILNSPLTVSSP
Sbjct: 181 EKEYHEAFDKLLEVLEVKSPFFLVVQGFLVGSYGLTWALKNPSKVEKLAILNSPLTVSSP 240

Query: 253 VPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYVLKLEKADVYRLPYLSSGGPGFALLE 312
           VPGLF+QLRIPLFGEFTCQNA++AERFIE GS YVLK EKADVYRLPYLSSGGPGFALLE
Sbjct: 241 VPGLFKQLRIPLFGEFTCQNAILAERFIEGGSPYVLKNEKADVYRLPYLSSGGPGFALLE 300

Query: 313 AARKANFNDILSRVTAGFASG 330
            A+K NF D LS++  GF+SG
Sbjct: 301 TAKKINFGDTLSQIANGFSSG 319

[Close]

BLAST of Bhi09G000005 vs. TAIR 10
Match: AT1G52510.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 392.5 bits (1007), Expect = 3.4e-109
Identity = 185/226 (81.86%), Postives = 203/226 (89.82%), Query Frame = 0


Query: 104 RIEDKGKEYGSYVKSGKFRWFVRETGSAKSRRGTIVFLHGAPTQSYSYRVVMSELSALGF 163
           R EDKGKEYGS +KSGK RWFVRETGS +SRRGTIVF+HGAPTQS+SYR VMSELS  GF
Sbjct: 2   RTEDKGKEYGSTIKSGKLRWFVRETGSKESRRGTIVFVHGAPTQSFSYRTVMSELSDAGF 61

Query: 164 HCFAPDWIGFGFSDKPQPGYGFNYTEKEYHEELDKLLDVLGVNTPFNLVIQGFLVGSYGL 223
           HCFAPDWIGFGFSDKPQPGYGFNYTEKEYHE  DKLL+VL V +PF LV+QGFLVGSYGL
Sbjct: 62  HCFAPDWIGFGFSDKPQPGYGFNYTEKEYHEAFDKLLEVLEVKSPFFLVVQGFLVGSYGL 121

Query: 224 TWALKNQSRISKISILNSPLTVSSPVPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYV 283
           TWALKN S++ K++ILNSPLTVSSPVPGLF+QLRIPLFGEFTCQNA++AERFIE GS YV
Sbjct: 122 TWALKNPSKVEKLAILNSPLTVSSPVPGLFKQLRIPLFGEFTCQNAILAERFIEGGSPYV 181

Query: 284 LKLEKADVYRLPYLSSGGPGFALLEAARKANFNDILSRVTAGFASG 330
           LK EKADVYRLPYLSSGGPGFALLE A+K NF D LS++  GF+SG
Sbjct: 182 LKNEKADVYRLPYLSSGGPGFALLETAKKINFGDTLSQIANGFSSG 227

[Close]

BLAST of Bhi09G000005 vs. TAIR 10
Match: AT4G12830.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 124.4 bits (311), Expect = 1.7e-28
Identity = 73/193 (37.82%), Postives = 108/193 (55.96%), Query Frame = 0


Query: 121 FRWFVRETGSAKSRRGTIVFLHGAPTQSYSYRVVMSELSALGFHCFAPDWIGFGFSDKPQ 180
           FRWF  E+GS  S    ++ +HG P+Q+YSYR  +  LS   +   A DW+GFGFSDKPQ
Sbjct: 121 FRWFSVESGSVDS--PPVILIHGFPSQAYSYRKTIPVLSK-NYRAIAFDWLGFGFSDKPQ 180

Query: 181 PGYGFNYTEKEYHEELDKLLDVLGVNTPFNLVIQGFLVGSYGLTWALKNQSRISKISILN 240
            GYGFNYT  E+   L+  +D +   +  +LV+QG+   +  + +A     +I  + +LN
Sbjct: 181 AGYGFNYTMDEFVSSLESFIDEV-TTSKVSLVVQGYFSAAV-VKYARNRPDKIKNLILLN 240

Query: 241 SPLTVS-SPVPGLFQQLRIPLFGEFTCQNAVMA-ERFIEAGSAYVLKLEKADVYRLPYLS 300
            PLT   + +P         L GE   Q+ + A ++ + +   Y +K + A VYR PYL+
Sbjct: 241 PPLTPEHAKLPSTLSVFSNFLLGEIFSQDPLRASDKPLTSCGPYKMKEDDAMVYRRPYLT 300

Query: 301 SGGPGFALLEAAR 312
           SG  GFAL   +R
Sbjct: 301 SGSSGFALNAISR 308

[Close]

BLAST of Bhi09G000005 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 1.2e-05
Identity = 29/72 (40.28%), Postives = 39/72 (54.17%), Query Frame = 0


Query: 138 IVFLHGAPTQSYSYRVVMSELSALGFHCFAPDWIGFGFSDKPQPGYGFNYTEKEYHEELD 197
           I+F+HG P   YS+R  +   +ALG+   APD  G+G SD P      +YT      +L 
Sbjct: 27  ILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLRGYGDSDAPPSRE--SYTILHIVGDLV 86

Query: 198 KLLDVLGVNTPF 210
            LLD LGV+  F
Sbjct: 87  GLLDSLGVDRVF 96

[Close]

BLAST of Bhi09G000005 vs. TAIR 10
Match: AT4G15955.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 45.8 bits (107), Expect = 7.7e-05
Identity = 27/72 (37.50%), Postives = 37/72 (51.39%), Query Frame = 0


Query: 114 SYVKSGKFRWFVRE-----TGSAKSRRGTIVFLHGAPTQSYSYRVVMSELSALGFHCFAP 173
           S+VK       V E      G+   R   I+FLHG P   Y++R  M  LS+LG+   AP
Sbjct: 8   SFVKVNGITMHVAEKSPSVAGNGAIRPPVILFLHGFPELWYTWRHQMVALSSLGYRTIAP 67

Query: 174 DWIGFGFSDKPQ 181
           D  G+G +D P+
Sbjct: 68  DLRGYGDTDAPE 79

[Close]

BLAST of Bhi09G000005 vs. ExPASy Swiss-Prot
Match: Q8PDW8 (Cis-3-alkyl-4-alkyloxetan-2-one decarboxylase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=oleB PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 6.4e-12
Identity = 44/122 (36.07%), Postives = 66/122 (54.10%), Query Frame = 0


Query: 138 IVFLHGAPTQSYSYRVVMSELSALGFHCFAPDWIGFGFSDKP------QPGYGFNYTEKE 197
           +V LHG P+  Y +R ++S LS   + C  PD IG G SDKP      QP Y  +YT + 
Sbjct: 34  VVMLHGNPSWGYLWRHLVSGLSD-RYRCIVPDHIGMGLSDKPDDAPDAQPRY--DYTLQS 93

Query: 198 YHEELDKLLDVLGVNTPFNLVIQGFLVGSYGLTWALKNQSRISKISILNS---PLTVSSP 251
             ++LD+LL  LG+  P  L +  +  G  G  WAL + +++ ++ I N+   PL    P
Sbjct: 94  RVDDLDRLLQHLGITGPITLAVHDW-GGMIGFGWALSHHAQVKRLVITNTAAFPLPPEKP 151

[Close]

BLAST of Bhi09G000005 vs. ExPASy Swiss-Prot
Match: P64302 (Haloalkane dehalogenase 1 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=dhmA1 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 4.7e-07
Identity = 46/122 (37.70%), Postives = 61/122 (50.00%), Query Frame = 0


Query: 138 IVFLHGAPTQSYSYRVVMSELSALGFHCFAPDWIGFGFSDKPQPGYGFNYTE-----KEY 197
           IV LHG PT SY YR ++  LSA G    APD IGFG SDKP     + Y         +
Sbjct: 49  IVLLHGEPTWSYLYRTMIPPLSAAGHRVLAPDLIGFGRSDKPTRIEDYTYLRHVEWVTSW 108

Query: 198 HEELDKLLDVLGVNTPFNLVIQGFLVGS-YGLTWALKNQSRISKISILNSPLTVS---SP 251
            E LD L DV        L +Q +  GS  GL  A ++  RI+++ + N  L  +   +P
Sbjct: 109 FENLD-LHDV-------TLFVQDW--GSLIGLRIAAEHGDRIARLVVANGFLPAAQGRTP 160

[Close]

BLAST of Bhi09G000005 vs. ExPASy Swiss-Prot
Match: P9WMS2 (Haloalkane dehalogenase 1 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=dhmA1 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 4.7e-07
Identity = 46/122 (37.70%), Postives = 61/122 (50.00%), Query Frame = 0


Query: 138 IVFLHGAPTQSYSYRVVMSELSALGFHCFAPDWIGFGFSDKPQPGYGFNYTE-----KEY 197
           IV LHG PT SY YR ++  LSA G    APD IGFG SDKP     + Y         +
Sbjct: 49  IVLLHGEPTWSYLYRTMIPPLSAAGHRVLAPDLIGFGRSDKPTRIEDYTYLRHVEWVTSW 108

Query: 198 HEELDKLLDVLGVNTPFNLVIQGFLVGS-YGLTWALKNQSRISKISILNSPLTVS---SP 251
            E LD L DV        L +Q +  GS  GL  A ++  RI+++ + N  L  +   +P
Sbjct: 109 FENLD-LHDV-------TLFVQDW--GSLIGLRIAAEHGDRIARLVVANGFLPAAQGRTP 160

[Close]

BLAST of Bhi09G000005 vs. ExPASy Swiss-Prot
Match: P9WMS3 (Haloalkane dehalogenase 1 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=dhmA1 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 4.7e-07
Identity = 46/122 (37.70%), Postives = 61/122 (50.00%), Query Frame = 0


Query: 138 IVFLHGAPTQSYSYRVVMSELSALGFHCFAPDWIGFGFSDKPQPGYGFNYTE-----KEY 197
           IV LHG PT SY YR ++  LSA G    APD IGFG SDKP     + Y         +
Sbjct: 49  IVLLHGEPTWSYLYRTMIPPLSAAGHRVLAPDLIGFGRSDKPTRIEDYTYLRHVEWVTSW 108

Query: 198 HEELDKLLDVLGVNTPFNLVIQGFLVGS-YGLTWALKNQSRISKISILNSPLTVS---SP 251
            E LD L DV        L +Q +  GS  GL  A ++  RI+++ + N  L  +   +P
Sbjct: 109 FENLD-LHDV-------TLFVQDW--GSLIGLRIAAEHGDRIARLVVANGFLPAAQGRTP 160

[Close]

BLAST of Bhi09G000005 vs. ExPASy Swiss-Prot
Match: P22643 (Haloalkane dehalogenase OS=Xanthobacter autotrophicus OX=280 GN=dhlA PE=1 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 6.2e-07
Identity = 44/130 (33.85%), Postives = 64/130 (49.23%), Query Frame = 0


Query: 141 LHGAPTQSYSYRVVMSELSALGFHCFAPDWIGFGFSDKPQPGYGFNYTEKEYHEE-LDKL 200
           LHG PT SY YR ++   +  G    APD+ GFG SDKP     + +   E+H   L  L
Sbjct: 53  LHGEPTWSYLYRKMIPVFAESGARVIAPDFFGFGKSDKPVDEEDYTF---EFHRNFLLAL 112

Query: 201 LDVLGVNTPFNLVIQGFLVGSYGLTWALKNQSRISKISILNSPLTVSSPVPGLFQQLRIP 260
           ++ L +     LV+Q +  G  GLT  + + SR  ++ I+N+ L ++ PV         P
Sbjct: 113 IERLDLRN-ITLVVQDW-GGFLGLTLPMADPSRFKRLIIMNACL-MTDPVTQ-------P 169

Query: 261 LFGEFTCQNA 270
            F  F  Q A
Sbjct: 173 AFSAFVTQPA 169

[Close]

BLAST of Bhi09G000005 vs. ExPASy TrEMBL
Match: A0A0A0KA42 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G432390 PE=4 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 7.5e-173
Identity = 307/330 (93.03%), Postives = 315/330 (95.45%), Query Frame = 0


Query: 1   MEAMARAVLSQPLSLSSPYPLYSLSASAKSYCHFTATTTRIRPASAAGRITTSAKGKDET 60
           MEAMARAVLSQPLS SSPYPL SLSAS +SYC+FTA TTRIRP S+ GRI  SAKGKDE 
Sbjct: 1   MEAMARAVLSQPLSCSSPYPLSSLSASTRSYCYFTAATTRIRPPSSPGRIIASAKGKDEA 60

Query: 61  GDSDADSPAFNPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRIEDKGKEYGSYVKSGK 120
            D DADS  FNPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRIEDKGKEYGSYVK+GK
Sbjct: 61  NDGDADSTGFNPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRIEDKGKEYGSYVKAGK 120

Query: 121 FRWFVRETGSAKSRRGTIVFLHGAPTQSYSYRVVMSELSALGFHCFAPDWIGFGFSDKPQ 180
           FRWFVRETGSAKSRRGTIVFLHGAPTQSYSYRVVMSELSA GFHCFAPDWIGFGFSDKPQ
Sbjct: 121 FRWFVRETGSAKSRRGTIVFLHGAPTQSYSYRVVMSELSAFGFHCFAPDWIGFGFSDKPQ 180

Query: 181 PGYGFNYTEKEYHEELDKLLDVLGVNTPFNLVIQGFLVGSYGLTWALKNQSRISKISILN 240
           PGYGFNYTEKEYHE LDKLLD+LG+NTPFNLVIQGFLVGSYGLTWALKNQSRISKISILN
Sbjct: 181 PGYGFNYTEKEYHEALDKLLDMLGINTPFNLVIQGFLVGSYGLTWALKNQSRISKISILN 240

Query: 241 SPLTVSSPVPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYVLKLEKADVYRLPYLSSG 300
           +PLTVSSPVPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYVLKLEKADVYRLPYLSSG
Sbjct: 241 TPLTVSSPVPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYVLKLEKADVYRLPYLSSG 300

Query: 301 GPGFALLEAARKANFNDILSRVTAGFASGR 331
           GPGFALLEAARKANFNDILSR+TAGFASGR
Sbjct: 301 GPGFALLEAARKANFNDILSRITAGFASGR 330

[Close]

BLAST of Bhi09G000005 vs. ExPASy TrEMBL
Match: A0A1S3CF87 (haloalkane dehalogenase OS=Cucumis melo OX=3656 GN=LOC103499813 PE=4 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 7.5e-173
Identity = 309/330 (93.64%), Postives = 314/330 (95.15%), Query Frame = 0


Query: 1   MEAMARAVLSQPLSLSSPYPLYSLSASAKSYCHFTATTTRIRPASAAGRITTSAKGKDET 60
           MEAMARAVLSQPLSL SPYPL SLSAS +SYC+FTA TTRIRPASAAGRI  SAKGKDE 
Sbjct: 1   MEAMARAVLSQPLSLPSPYPLSSLSASTRSYCYFTAVTTRIRPASAAGRIIASAKGKDEM 60

Query: 61  GDSDADSPAFNPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRIEDKGKEYGSYVKSGK 120
            D DADS  FNPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRIEDKGKEYGSYVKSGK
Sbjct: 61  NDGDADSTGFNPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRIEDKGKEYGSYVKSGK 120

Query: 121 FRWFVRETGSAKSRRGTIVFLHGAPTQSYSYRVVMSELSALGFHCFAPDWIGFGFSDKPQ 180
            RWFVRETGSAKSRRGTIVFLHGAPTQSYSYRVVMSEL+A GFHCFAPDWIGFGFSDKPQ
Sbjct: 121 LRWFVRETGSAKSRRGTIVFLHGAPTQSYSYRVVMSELAAFGFHCFAPDWIGFGFSDKPQ 180

Query: 181 PGYGFNYTEKEYHEELDKLLDVLGVNTPFNLVIQGFLVGSYGLTWALKNQSRISKISILN 240
           PGYGFNYTEKEYHE LDKLLDVLG+NTPFNLVIQGFLVGSYGLTWALKNQSRISKISILN
Sbjct: 181 PGYGFNYTEKEYHEALDKLLDVLGINTPFNLVIQGFLVGSYGLTWALKNQSRISKISILN 240

Query: 241 SPLTVSSPVPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYVLKLEKADVYRLPYLSSG 300
           SPLTVSSPVPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYVLKLEKADVYRLPYLSSG
Sbjct: 241 SPLTVSSPVPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYVLKLEKADVYRLPYLSSG 300

Query: 301 GPGFALLEAARKANFNDILSRVTAGFASGR 331
           GPGFALLEA RKANFNDILSR+TAGFAS R
Sbjct: 301 GPGFALLEATRKANFNDILSRITAGFASER 330

[Close]

BLAST of Bhi09G000005 vs. ExPASy TrEMBL
Match: A0A5D3CJF7 (Haloalkane dehalogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001890 PE=4 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 4.1e-171
Identity = 306/330 (92.73%), Postives = 311/330 (94.24%), Query Frame = 0


Query: 1   MEAMARAVLSQPLSLSSPYPLYSLSASAKSYCHFTATTTRIRPASAAGRITTSAKGKDET 60
           MEAM RAVLSQPLSL SPYPL SLSAS +SYC+FTA TTRIRPASAAGRI   AKGKDE 
Sbjct: 1   MEAMPRAVLSQPLSLPSPYPLSSLSASTRSYCYFTAVTTRIRPASAAGRIIAGAKGKDEM 60

Query: 61  GDSDADSPAFNPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRIEDKGKEYGSYVKSGK 120
            D DADS  FNPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRIEDKGKEYGSYVKSGK
Sbjct: 61  NDGDADSTGFNPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRIEDKGKEYGSYVKSGK 120

Query: 121 FRWFVRETGSAKSRRGTIVFLHGAPTQSYSYRVVMSELSALGFHCFAPDWIGFGFSDKPQ 180
            RWFVRETGSAKSRRGTIVFLHGAPTQSYSYRVVMSEL+A GFHCFAPDWIGFGFSDKPQ
Sbjct: 121 LRWFVRETGSAKSRRGTIVFLHGAPTQSYSYRVVMSELAAFGFHCFAPDWIGFGFSDKPQ 180

Query: 181 PGYGFNYTEKEYHEELDKLLDVLGVNTPFNLVIQGFLVGSYGLTWALKNQSRISKISILN 240
           PGYGFNYTEKEYHE LDKLLDVLG+NTPFNLVIQGFLVGSYGLTWALKNQSRISKISILN
Sbjct: 181 PGYGFNYTEKEYHEALDKLLDVLGINTPFNLVIQGFLVGSYGLTWALKNQSRISKISILN 240

Query: 241 SPLTVSSPVPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYVLKLEKADVYRLPYLSSG 300
           SPLTVSSPVPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYVLKLEKADVYRLPYLSSG
Sbjct: 241 SPLTVSSPVPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYVLKLEKADVYRLPYLSSG 300

Query: 301 GPGFALLEAARKANFNDILSRVTAGFASGR 331
           GPGFALLEA RKANFNDILSR+TAGF S R
Sbjct: 301 GPGFALLEATRKANFNDILSRITAGFDSER 330

[Close]

BLAST of Bhi09G000005 vs. ExPASy TrEMBL
Match: A0A6J1GPT3 (uncharacterized protein LOC111456363 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456363 PE=4 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.6e-170
Identity = 303/327 (92.66%), Postives = 317/327 (96.94%), Query Frame = 0


Query: 4   MARAVLSQPLSLSSPYPLYSLSASAKSYCHFTATTTRIRPASAAGRITTSAKGKDETGDS 63
           MARAVLSQPLS+ S YPL SLSAS KS+ +FT TTTRIRPA+A GR++ SAKGKDETGD+
Sbjct: 1   MARAVLSQPLSVPSSYPLSSLSASTKSHYYFTPTTTRIRPATAGGRVSVSAKGKDETGDA 60

Query: 64  DADSPAFNPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRIEDKGKEYGSYVKSGKFRW 123
           DADSP F+PFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRIEDKGKEYGSYVKSGKFRW
Sbjct: 61  DADSPGFSPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRIEDKGKEYGSYVKSGKFRW 120

Query: 124 FVRETGSAKSRRGTIVFLHGAPTQSYSYRVVMSELSALGFHCFAPDWIGFGFSDKPQPGY 183
           FVRETG+A SRRGTIVFLHGAPTQSYSYRVVM+ELSALGFHCFAPDWIGFGFSDKPQPGY
Sbjct: 121 FVRETGAANSRRGTIVFLHGAPTQSYSYRVVMNELSALGFHCFAPDWIGFGFSDKPQPGY 180

Query: 184 GFNYTEKEYHEELDKLLDVLGVNTPFNLVIQGFLVGSYGLTWALKNQSRISKISILNSPL 243
           GFNYTEKEYHEELDKLLDVLGV++PFNLVIQGFLVGSYGLTWALKNQSRISKISILNSPL
Sbjct: 181 GFNYTEKEYHEELDKLLDVLGVDSPFNLVIQGFLVGSYGLTWALKNQSRISKISILNSPL 240

Query: 244 TVSSPVPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYVLKLEKADVYRLPYLSSGGPG 303
           TVSSPVPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYVLKLEKADVYRLPYLSSGGPG
Sbjct: 241 TVSSPVPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYVLKLEKADVYRLPYLSSGGPG 300

Query: 304 FALLEAARKANFNDILSRVTAGFASGR 331
           FALLEAARKANFNDILSR++AGFASGR
Sbjct: 301 FALLEAARKANFNDILSRISAGFASGR 327

[Close]

BLAST of Bhi09G000005 vs. ExPASy TrEMBL
Match: A0A6J1JUI5 (uncharacterized protein LOC111488487 OS=Cucurbita maxima OX=3661 GN=LOC111488487 PE=4 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.0e-170
Identity = 303/327 (92.66%), Postives = 315/327 (96.33%), Query Frame = 0


Query: 4   MARAVLSQPLSLSSPYPLYSLSASAKSYCHFTATTTRIRPASAAGRITTSAKGKDETGDS 63
           MAR VLSQPLS+ S YPL SLSAS KSY +FT  TTRIRPA+A GRIT SAKGKDETG++
Sbjct: 1   MARVVLSQPLSVPSSYPLSSLSASTKSYYYFTPATTRIRPATAGGRITVSAKGKDETGEA 60

Query: 64  DADSPAFNPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRIEDKGKEYGSYVKSGKFRW 123
           D+DSP FNPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRIEDKGKEYGSYVKSGKFRW
Sbjct: 61  DSDSPGFNPFGFVTDNPSSRSAIQLPESPAEDGNVGQMLYRIEDKGKEYGSYVKSGKFRW 120

Query: 124 FVRETGSAKSRRGTIVFLHGAPTQSYSYRVVMSELSALGFHCFAPDWIGFGFSDKPQPGY 183
           FVRETG+A SRRGTIVFLHGAPTQSYSYRVVM+ELSALGFHCFAPDWIGFGFSDKPQPGY
Sbjct: 121 FVRETGAANSRRGTIVFLHGAPTQSYSYRVVMNELSALGFHCFAPDWIGFGFSDKPQPGY 180

Query: 184 GFNYTEKEYHEELDKLLDVLGVNTPFNLVIQGFLVGSYGLTWALKNQSRISKISILNSPL 243
           GFNYTEKEYHEELDKLLDVLGV++PFNLVIQGFLVGSYGLTWALKNQSRISKISILNSPL
Sbjct: 181 GFNYTEKEYHEELDKLLDVLGVDSPFNLVIQGFLVGSYGLTWALKNQSRISKISILNSPL 240

Query: 244 TVSSPVPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYVLKLEKADVYRLPYLSSGGPG 303
           TVSSPVPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYVLKLEKADVYRLPYLSSGGPG
Sbjct: 241 TVSSPVPGLFQQLRIPLFGEFTCQNAVMAERFIEAGSAYVLKLEKADVYRLPYLSSGGPG 300

Query: 304 FALLEAARKANFNDILSRVTAGFASGR 331
           FALLEAARKANFNDILSR++AGFASGR
Sbjct: 301 FALLEAARKANFNDILSRISAGFASGR 327

The following BLAST results are available for this feature:

BLAST of Bhi09G000005 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52510.1	3.7e-132	74.14	alpha/beta-Hydrolases superfamily protein	[more]
AT1G52510.2	3.4e-109	81.86	alpha/beta-Hydrolases superfamily protein	[more]
AT4G12830.1	1.7e-28	37.82	alpha/beta-Hydrolases superfamily protein	[more]
AT4G02340.1	1.2e-05	40.28	alpha/beta-Hydrolases superfamily protein	[more]
AT4G15955.1	7.7e-05	37.50	alpha/beta-Hydrolases superfamily protein	[more]

BLAST of Bhi09G000005 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8PDW8	6.4e-12	36.07	Cis-3-alkyl-4-alkyloxetan-2-one decarboxylase OS=Xanthomonas campestris pv. camp...	[more]
P64302	4.7e-07	37.70	Haloalkane dehalogenase 1 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/9...	[more]
P9WMS2	4.7e-07	37.70	Haloalkane dehalogenase 1 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshko...	[more]
P9WMS3	4.7e-07	37.70	Haloalkane dehalogenase 1 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37...	[more]
P22643	6.2e-07	33.85	Haloalkane dehalogenase OS=Xanthobacter autotrophicus OX=280 GN=dhlA PE=1 SV=2	[more]

BLAST of Bhi09G000005 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KA42	7.5e-173	93.03	AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G432...	[more]
A0A1S3CF87	7.5e-173	93.64	haloalkane dehalogenase OS=Cucumis melo OX=3656 GN=LOC103499813 PE=4 SV=1	[more]
A0A5D3CJF7	4.1e-171	92.73	Haloalkane dehalogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A6J1GPT3	1.6e-170	92.66	uncharacterized protein LOC111456363 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1JUI5	2.0e-170	92.66	uncharacterized protein LOC111488487 OS=Cucurbita maxima OX=3661 GN=LOC111488487...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 109..331 e-value: 1.3E-27 score: 99.4
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 114..307
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF00561	Abhydrolase_1	coord: 137..247 e-value: 2.3E-8 score: 34.0
None	No IPR available	PANTHER	PTHR43194:SF2	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 111..325
None	No IPR available	PANTHER	PTHR43194	HYDROLASE ALPHA/BETA FOLD FAMILY	coord: 111..325

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M000005	Bhi09M000005	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity