Bhi08G001772 (gene) Wax gourd (B227) v1

Overview


Name	Bhi08G001772
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	WRKY protein
Location	chr8: 61842124 .. 61843934 (-)
RNA-Seq Expression	Bhi08G001772
Synteny	Bhi08G001772

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCTTCTTCAAATCCCTCTGGTACAAACTTTTCCGACAGCCATTAACCTTAAAAAACCACCACCCATCTGTGGCGGGCGGCCATAAATACTATGGATGATGTTTCCTTCAACAATAATAATAACCACCAATTCTATTCTGCTGATCTATTTGAAGATTCCGACGAATTAAAACCCACCCCCGATTCTCCTCCTCGTAATTCCGGCGCCGCCACCGCCACGAAGAAGGGCAGGTGAGTCTTCTATTATTAGTTAAACCCAATTCAAATGGGTTTTATTTTATTTTAATTTAATTTATGGTGTGTGTATTTAGAAGAGGGATGAAGAAGAAGGTTGTGTCAGTGAAAATTAATAGCGACAGCCCAAGAAATTCCTCTGGCTCTGCACCTCCACCTTCTGATTCTTGGGCGTGGAGAAAGTATGGTCAAAAGCCCATCAAAGGCTCTCCTTACCCCAGGTTTGATTTAGTTTTGAATTTCCAGGTTTGAAATGGGAATTAATAAACTGAGAGCTATACTCGAATTGATGGGTTTTGGGTTTAATTTTTGTAGGGCGTATTATAGGTGTAGTAGCTCAAAGGGATGTCCAGCAAGGAAGCAAGTAGAGAAGAATCGACTTGATCCAACCACGTTGGTCATTACATATTCGTGTGAGCATAACCATTCCGGTCCGGTTTCAAGAAACAACAATAACAATAACAACAATCACAACCAAACCGCTGCGGCTGCGCTGAAACCCGGTTCACCAGAGACGGTTCCAGTTCATCAAGAACCGGAAGTGGAAGAGAAATTTGTGGAGATAGTGGGAGAGGAGTCGTTCATAACCGGGGATGAGTTTAGTTGGTTTGGAGAAATGGAAACGACGTCGTCTACAGTGCTTGAAAGCTCCATTTTCTCAGGGAGAGGATCGAGTGGATTGGTAGATCATTCCATTTCAAGTGACGTGGCAATGCTTTTTCCGATGGGTGATGATGACGTGGATGAGTCACTTTTTGCGGATCTCGGGGAGTTGCCCGAGTGTTCCCTTGTGTTCCGACGCGGCGGCGGTCGTGGATTACCGCTGGATGAACAACCGGCTGCAACCCAGCGGCGGATCACGCCCTGGTGTGGAACCACCACATGAGATGAGACGTGCACCTCTCACTTTGTTTGTATACTTCTAGGAGCATCCTCCGCCATTGATGCCGGGAAAGTTTTCTTTTTTTCTTTTTTTTTTTAAACTTCGTTTTCTTTCTTTTTATATATTTTTTCGAATATGCAATATAAAAAAAAAAAAAATAGAAAGAAAGAAAATCTTGTTTCATTTTATTCTAAGGTTAAATTACCATTTTAATCCATCCTTATACTTTCTGTTGAATTCTAATTTGAGCTATATCGTTTTAAAATTTTCAATTTAGGCACCTATGATTTGATTAAAATTTTCAAAATATTATTCTTATTACCAAACCTTAATCCCACAGTGACAAATTTTCTTCAAATGTTGAATTGATGTTTCAAGAGAATATGTCACGTTAAAAAGGTGTTATTGGTTTGGATAAAGGTAATAATGCTTTGGGAATTTTGATCAAATCTTATGGGGTTCGCAATAAGTTGAAACTTTTAAAATAACTAAAATGGTAATTTAACCTTACCAATAGTTATCCATCTTTGTGTAATTTGGGTGATATTTTGTCTTCCTATTAAATACTTCTTGCATGCAATTTATGTATATATACCCACACGGTCATAAATTTTTATGGCTTTGTTTTCTCCATCTCATTAATTAATCAAATTAAAATATCTTCTATATCTATCTGTATTTTGACACGTTAATT

mRNA sequence

CTCTTCTTCAAATCCCTCTGGTACAAACTTTTCCGACAGCCATTAACCTTAAAAAACCACCACCCATCTGTGGCGGGCGGCCATAAATACTATGGATGATGTTTCCTTCAACAATAATAATAACCACCAATTCTATTCTGCTGATCTATTTGAAGATTCCGACGAATTAAAACCCACCCCCGATTCTCCTCCTCGTAATTCCGGCGCCGCCACCGCCACGAAGAAGGGCAGAAGAGGGATGAAGAAGAAGGTTGTGTCAGTGAAAATTAATAGCGACAGCCCAAGAAATTCCTCTGGCTCTGCACCTCCACCTTCTGATTCTTGGGCGTGGAGAAAGTATGGTCAAAAGCCCATCAAAGGCTCTCCTTACCCCAGGGCGTATTATAGGTGTAGTAGCTCAAAGGGATGTCCAGCAAGGAAGCAAGTAGAGAAGAATCGACTTGATCCAACCACGTTGGTCATTACATATTCGTGTGAGCATAACCATTCCGGTCCGGTTTCAAGAAACAACAATAACAATAACAACAATCACAACCAAACCGCTGCGGCTGCGCTGAAACCCGGTTCACCAGAGACGGTTCCAGTTCATCAAGAACCGGAAGTGGAAGAGAAATTTGTGGAGATAGTGGGAGAGGAGTCGTTCATAACCGGGGATGAGTTTAGTTGGTTTGGAGAAATGGAAACGACGTCGTCTACAGTGCTTGAAAGCTCCATTTTCTCAGGGAGAGGATCGAGTGGATTGGTAGATCATTCCATTTCAAGTGACGTGGCAATGCTTTTTCCGATGGGTGATGATGACGTGGATGAGTCACTTTTTGCGGATCTCGGGGAGTTGCCCGAGTGTTCCCTTGTGTTCCGACGCGGCGGCGGTCGTGGATTACCGCTGGATGAACAACCGGCTGCAACCCAGCGGCGGATCACGCCCTGGTGTGGAACCACCACATGAGATGAGACGTGCACCTCTCACTTTGTTTGTATACTTCTAGGAGCATCCTCCGCCATTGATGCCGGGAAAGTTTTCTTTTTTTCTTTTTTTTTTTAAACTTCGTTTTCTTTCTTTTTATATATTTTTTCGAATATGCAATATAAAAAAAAAAAAAATAGAAAGAAAGAAAATCTTGTTTCATTTTATTCTAAGGTTAAATTACCATTTTAATCCATCCTTATACTTTCTGTTGAATTCTAATTTGAGCTATATCGTTTTAAAATTTTCAATTTAGGCACCTATGATTTGATTAAAATTTTCAAAATATTATTCTTATTACCAAACCTTAATCCCACAGTGACAAATTTTCTTCAAATGTTGAATTGATGTTTCAAGAGAATATGTCACGTTAAAAAGGTGTTATTGGTTTGGATAAAGGTAATAATGCTTTGGGAATTTTGATCAAATCTTATGGGGTTCGCAATAAGTTGAAACTTTTAAAATAACTAAAATGGTAATTTAACCTTACCAATAGTTATCCATCTTTGTGTAATTTGGGTGATATTTTGTCTTCCTATTAAATACTTCTTGCATGCAATTTATGTATATATACCCACACGGTCATAAATTTTTATGGCTTTGTTTTCTCCATCTCATTAATTAATCAAATTAAAATATCTTCTATATCTATCTGTATTTTGACACGTTAATT

Coding sequence (CDS)

ATGGATGATGTTTCCTTCAACAATAATAATAACCACCAATTCTATTCTGCTGATCTATTTGAAGATTCCGACGAATTAAAACCCACCCCCGATTCTCCTCCTCGTAATTCCGGCGCCGCCACCGCCACGAAGAAGGGCAGAAGAGGGATGAAGAAGAAGGTTGTGTCAGTGAAAATTAATAGCGACAGCCCAAGAAATTCCTCTGGCTCTGCACCTCCACCTTCTGATTCTTGGGCGTGGAGAAAGTATGGTCAAAAGCCCATCAAAGGCTCTCCTTACCCCAGGGCGTATTATAGGTGTAGTAGCTCAAAGGGATGTCCAGCAAGGAAGCAAGTAGAGAAGAATCGACTTGATCCAACCACGTTGGTCATTACATATTCGTGTGAGCATAACCATTCCGGTCCGGTTTCAAGAAACAACAATAACAATAACAACAATCACAACCAAACCGCTGCGGCTGCGCTGAAACCCGGTTCACCAGAGACGGTTCCAGTTCATCAAGAACCGGAAGTGGAAGAGAAATTTGTGGAGATAGTGGGAGAGGAGTCGTTCATAACCGGGGATGAGTTTAGTTGGTTTGGAGAAATGGAAACGACGTCGTCTACAGTGCTTGAAAGCTCCATTTTCTCAGGGAGAGGATCGAGTGGATTGGTAGATCATTCCATTTCAAGTGACGTGGCAATGCTTTTTCCGATGGGTGATGATGACGTGGATGAGTCACTTTTTGCGGATCTCGGGGAGTTGCCCGAGTGTTCCCTTGTGTTCCGACGCGGCGGCGGTCGTGGATTACCGCTGGATGAACAACCGGCTGCAACCCAGCGGCGGATCACGCCCTGGTGTGGAACCACCACATGA

Protein sequence

MDDVSFNNNNNHQFYSADLFEDSDELKPTPDSPPRNSGAATATKKGRRGMKKKVVSVKINSDSPRNSSGSAPPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVEKNRLDPTTLVITYSCEHNHSGPVSRNNNNNNNNHNQTAAAALKPGSPETVPVHQEPEVEEKFVEIVGEESFITGDEFSWFGEMETTSSTVLESSIFSGRGSSGLVDHSISSDVAMLFPMGDDDVDESLFADLGELPECSLVFRRGGGRGLPLDEQPAATQRRITPWCGTTT

Homology

[Close]

BLAST of Bhi08G001772 vs. TAIR 10
Match: AT1G29280.1 (WRKY DNA-binding protein 65 )

HSP 1 Score: 194.9 bits (494), Expect = 8.8e-50
Identity = 122/245 (49.80%), Postives = 154/245 (62.86%), Query Frame = 0


Query: 21  EDSDELKP-TPDSPPRNSGAAT----ATKKGRRGMKKKVVSVKINSDSPRNSSGSAPPPS 80
           E S E  P +P+S   N   A     + K+ RR ++K+VV+V +         G   PPS
Sbjct: 15  ESSQETGPESPNSSTFNGMKALISSHSPKRSRRSVEKRVVNVPMKEMEGSRHKGDTTPPS 74

Query: 81  DSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVEKNRLDPTTLVITYSCEHNHSGP 140
           DSWAWRKYGQKPIKGSPYPR YYRCSS+KGCPARKQVE++R DPT ++ITY+ EHNH  P
Sbjct: 75  DSWAWRKYGQKPIKGSPYPRGYYRCSSTKGCPARKQVERSRDDPTMILITYTSEHNHPWP 134

Query: 141 VSRNNNNNNNNHNQTAAAALKPGSPETVP-VHQEPEVEEKFVEIVG---EESFITGDEFS 200
           ++ +  N      +          PE  P V  E E E+    ++G   E +    DEF+
Sbjct: 135 LTSSTRNGPKPKPEP--------KPEPEPEVEPEAEEEDNKFMVLGRGIETTPSCVDEFA 194

Query: 201 WFGEMETTSSTVLESSIFSGRGSSGLVDHSISSDVAMLFPMGDDDVDESLFADLGELPEC 257
           WF EMETTSST+LES IFS    + +   S + DVA+ FPMG++  DESLFADLGELPEC
Sbjct: 195 WFTEMETTSSTILESPIFSSEKKTAV---SGADDVAVFFPMGEE--DESLFADLGELPEC 246

[Close]

BLAST of Bhi08G001772 vs. TAIR 10
Match: AT3G58710.1 (WRKY DNA-binding protein 69 )

HSP 1 Score: 169.1 bits (427), Expect = 5.2e-42
Identity = 113/269 (42.01%), Postives = 148/269 (55.02%), Query Frame = 0


Query: 21  EDSDELKPTPDSPPRNSGAATATKKGRRGMKKKVVSVKINSDSPRNSSGSAPPPSDSWAW 80
           EDS   KPTP             KK RR ++K+VVSV I       S G   PPSDSWAW
Sbjct: 30  EDSKISKPTP-------------KKSRRNVEKRVVSVPIADVEGSKSRGEVYPPSDSWAW 89

Query: 81  RKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVEKNRLDPTTLVITYSCEHNHSGPVSRNN 140
           RKYGQKPIKGSPYPR YYRCSSSKGCPARKQVE++R+DP+ L+ITY+C+HNH  P S  N
Sbjct: 90  RKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMITYACDHNHPFPSSSAN 149

Query: 141 NNNNNNHNQTAAAALKPGSPE------TVPVHQEPEVEEKFVEIVGEESFITGDEFSWFG 200
             +++  +     A K    E      TV   +EP        +  +   + G  +S  G
Sbjct: 150 TKSHHRSSVVLKTAKKEEEYEEEEEELTVTAAEEPPAGLDLSHV--DSPLLLGGCYSEIG 209

Query: 201 EMETTSSTVLESSIFSGRGSSGLVDHSISSDVAMLFPMGDDDVDESLFADLGELPECSLV 260
           E         ++SI S  GSS  +D ++       F +G ++ DESLF DLG+LP+C+ V
Sbjct: 210 EF----GWFYDASISSSSGSSNFLDVTLERG----FSVGQEE-DESLFGDLGDLPDCASV 269

Query: 261 FRRGGGRGLPLDEQPAATQRRITPWCGTT 284
           FRRG    +  +EQ         P+C ++
Sbjct: 270 FRRG---TVATEEQHRRCDFGAIPFCDSS 271

[Close]

BLAST of Bhi08G001772 vs. TAIR 10
Match: AT3G58710.2 (WRKY DNA-binding protein 69 )

HSP 1 Score: 166.8 bits (421), Expect = 2.6e-41
Identity = 110/269 (40.89%), Postives = 150/269 (55.76%), Query Frame = 0


Query: 21  EDSDELKPTPDSPPRNSGAATATKKGRRGMKKKVVSVKINSDSPRNSSGSAPPPSDSWAW 80
           E  +++ P   S   +S  +  T K RR ++K+VVSV I       S G   PPSDSWAW
Sbjct: 16  ETYNDVVPESPSSCEDSKISKPTPKKRRNVEKRVVSVPIADVEGSKSRGEVYPPSDSWAW 75

Query: 81  RKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVEKNRLDPTTLVITYSCEHNHSGPVSRNN 140
           RKYGQKPIKGSPYPR YYRCSSSKGCPARKQVE++R+DP+ L+ITY+C+HNH  P S  N
Sbjct: 76  RKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMITYACDHNHPFPSSSAN 135

Query: 141 NNNNNNHNQTAAAALKPGSPE------TVPVHQEPEVEEKFVEIVGEESFITGDEFSWFG 200
             +++  +     A K    E      TV   +EP        +  +   + G  +S  G
Sbjct: 136 TKSHHRSSVVLKTAKKEEEYEEEEEELTVTAAEEPPAGLDLSHV--DSPLLLGGCYSEIG 195

Query: 201 EMETTSSTVLESSIFSGRGSSGLVDHSISSDVAMLFPMGDDDVDESLFADLGELPECSLV 260
           E         ++SI S  GSS  +D ++       F +G ++ DESLF DLG+LP+C+ V
Sbjct: 196 EF----GWFYDASISSSSGSSNFLDVTLERG----FSVGQEE-DESLFGDLGDLPDCASV 255

Query: 261 FRRGGGRGLPLDEQPAATQRRITPWCGTT 284
           FRRG    +  +EQ         P+C ++
Sbjct: 256 FRRG---TVATEEQHRRCDFGAIPFCDSS 270

[Close]

BLAST of Bhi08G001772 vs. TAIR 10
Match: AT1G30650.1 (WRKY DNA-binding protein 14 )

HSP 1 Score: 122.9 bits (307), Expect = 4.2e-28
Identity = 90/236 (38.14%), Postives = 117/236 (49.58%), Query Frame = 0


Query: 32  SPPRNSGAATATKKGRRGMKKKVVSVKINSDSPRNSSGSAPPPSDSWAWRKYGQKPIKGS 91
           S PRN G      K R+   KKVV +   +     SSG    PSD WAWRKYGQKPIKGS
Sbjct: 180 SSPRNLGL-----KRRKSQAKKVVCIPAPAAMNSRSSGEV-VPSDLWAWRKYGQKPIKGS 239

Query: 92  PYPRAYYRCSSSKGCPARKQVEKNRLDPTTLVITYSCEHNHSGPVSRNNNNNNNNHNQTA 151
           P+PR YYRCSSSKGC ARKQVE++R DP  LVITY+ EHNH  P+ RN    +   + ++
Sbjct: 240 PFPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGSTRSSTSS 299

Query: 152 AAALKPGSPETVPVHQEPEVEEKFVEIVGEESFITGDEFSWFGEMETTSSTVLESSIFSG 211
           ++   P  P T  V+            +G ++ I      +     T   T+  S+I   
Sbjct: 300 SSNPNPSKPSTANVNSSS---------IGSQNTI------YLPSSTTPPPTLSSSAIKDE 359

Query: 212 RGSSGLVDHSISSDVAMLFPMGDDDVD-----ESLFADLGELPECSLVFRRGGGRG 263
           RG    +++    D   + P   +  D     +  FADL EL   SL      G G
Sbjct: 360 RGDDMELENVDDDDDNQIAPYRPELHDHQHQPDDFFADLEELEGDSLSMLLSHGCG 394

[Close]

BLAST of Bhi08G001772 vs. TAIR 10
Match: AT2G34830.1 (WRKY DNA-binding protein 35 )

HSP 1 Score: 122.1 bits (305), Expect = 7.2e-28
Identity = 73/139 (52.52%), Postives = 84/139 (60.43%), Query Frame = 0


Query: 32  SPPRNSGAATATKKGRRGMKKKVVSVKINSDSPRNSSGSAPPPSDSWAWRKYGQKPIKGS 91
           S PRN G      K R+   KKVV +   +     SSG    PSD WAWRKYGQKPIKGS
Sbjct: 178 SSPRNLGI-----KRRKSQAKKVVCIPAPAAMNSRSSGEV-VPSDLWAWRKYGQKPIKGS 237

Query: 92  PYPRAYYRCSSSKGCPARKQVEKNRLDPTTLVITYSCEHNHSGPVSRN-----------N 151
           PYPR YYRCSSSKGC ARKQVE++R DP  LVITY+ EHNH  P  RN           +
Sbjct: 238 PYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSS 297

Query: 152 NNNNNNHNQTAAAALKPGS 160
           + N ++ + TAAA   P S
Sbjct: 298 SLNPSSKSSTAAATTSPSS 310

[Close]

BLAST of Bhi08G001772 vs. ExPASy Swiss-Prot
Match: Q9LP56 (Probable WRKY transcription factor 65 OS=Arabidopsis thaliana OX=3702 GN=WRKY65 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.2e-48
Identity = 122/245 (49.80%), Postives = 154/245 (62.86%), Query Frame = 0


Query: 21  EDSDELKP-TPDSPPRNSGAAT----ATKKGRRGMKKKVVSVKINSDSPRNSSGSAPPPS 80
           E S E  P +P+S   N   A     + K+ RR ++K+VV+V +         G   PPS
Sbjct: 15  ESSQETGPESPNSSTFNGMKALISSHSPKRSRRSVEKRVVNVPMKEMEGSRHKGDTTPPS 74

Query: 81  DSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVEKNRLDPTTLVITYSCEHNHSGP 140
           DSWAWRKYGQKPIKGSPYPR YYRCSS+KGCPARKQVE++R DPT ++ITY+ EHNH  P
Sbjct: 75  DSWAWRKYGQKPIKGSPYPRGYYRCSSTKGCPARKQVERSRDDPTMILITYTSEHNHPWP 134

Query: 141 VSRNNNNNNNNHNQTAAAALKPGSPETVP-VHQEPEVEEKFVEIVG---EESFITGDEFS 200
           ++ +  N      +          PE  P V  E E E+    ++G   E +    DEF+
Sbjct: 135 LTSSTRNGPKPKPEP--------KPEPEPEVEPEAEEEDNKFMVLGRGIETTPSCVDEFA 194

Query: 201 WFGEMETTSSTVLESSIFSGRGSSGLVDHSISSDVAMLFPMGDDDVDESLFADLGELPEC 257
           WF EMETTSST+LES IFS    + +   S + DVA+ FPMG++  DESLFADLGELPEC
Sbjct: 195 WFTEMETTSSTILESPIFSSEKKTAV---SGADDVAVFFPMGEE--DESLFADLGELPEC 246

[Close]

BLAST of Bhi08G001772 vs. ExPASy Swiss-Prot
Match: Q93WV5 (Probable WRKY transcription factor 69 OS=Arabidopsis thaliana OX=3702 GN=WRKY69 PE=2 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 3.6e-40
Identity = 110/269 (40.89%), Postives = 150/269 (55.76%), Query Frame = 0


Query: 21  EDSDELKPTPDSPPRNSGAATATKKGRRGMKKKVVSVKINSDSPRNSSGSAPPPSDSWAW 80
           E  +++ P   S   +S  +  T K RR ++K+VVSV I       S G   PPSDSWAW
Sbjct: 16  ETYNDVVPESPSSCEDSKISKPTPKKRRNVEKRVVSVPIADVEGSKSRGEVYPPSDSWAW 75

Query: 81  RKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVEKNRLDPTTLVITYSCEHNHSGPVSRNN 140
           RKYGQKPIKGSPYPR YYRCSSSKGCPARKQVE++R+DP+ L+ITY+C+HNH  P S  N
Sbjct: 76  RKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMITYACDHNHPFPSSSAN 135

Query: 141 NNNNNNHNQTAAAALKPGSPE------TVPVHQEPEVEEKFVEIVGEESFITGDEFSWFG 200
             +++  +     A K    E      TV   +EP        +  +   + G  +S  G
Sbjct: 136 TKSHHRSSVVLKTAKKEEEYEEEEEELTVTAAEEPPAGLDLSHV--DSPLLLGGCYSEIG 195

Query: 201 EMETTSSTVLESSIFSGRGSSGLVDHSISSDVAMLFPMGDDDVDESLFADLGELPECSLV 260
           E         ++SI S  GSS  +D ++       F +G ++ DESLF DLG+LP+C+ V
Sbjct: 196 EF----GWFYDASISSSSGSSNFLDVTLERG----FSVGQEE-DESLFGDLGDLPDCASV 255

Query: 261 FRRGGGRGLPLDEQPAATQRRITPWCGTT 284
           FRRG    +  +EQ         P+C ++
Sbjct: 256 FRRG---TVATEEQHRRCDFGAIPFCDSS 270

[Close]

BLAST of Bhi08G001772 vs. ExPASy Swiss-Prot
Match: Q9SA80 (Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 PE=2 SV=2)

HSP 1 Score: 122.9 bits (307), Expect = 6.0e-27
Identity = 90/236 (38.14%), Postives = 117/236 (49.58%), Query Frame = 0


Query: 32  SPPRNSGAATATKKGRRGMKKKVVSVKINSDSPRNSSGSAPPPSDSWAWRKYGQKPIKGS 91
           S PRN G      K R+   KKVV +   +     SSG    PSD WAWRKYGQKPIKGS
Sbjct: 180 SSPRNLGL-----KRRKSQAKKVVCIPAPAAMNSRSSGEV-VPSDLWAWRKYGQKPIKGS 239

Query: 92  PYPRAYYRCSSSKGCPARKQVEKNRLDPTTLVITYSCEHNHSGPVSRNNNNNNNNHNQTA 151
           P+PR YYRCSSSKGC ARKQVE++R DP  LVITY+ EHNH  P+ RN    +   + ++
Sbjct: 240 PFPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGSTRSSTSS 299

Query: 152 AAALKPGSPETVPVHQEPEVEEKFVEIVGEESFITGDEFSWFGEMETTSSTVLESSIFSG 211
           ++   P  P T  V+            +G ++ I      +     T   T+  S+I   
Sbjct: 300 SSNPNPSKPSTANVNSSS---------IGSQNTI------YLPSSTTPPPTLSSSAIKDE 359

Query: 212 RGSSGLVDHSISSDVAMLFPMGDDDVD-----ESLFADLGELPECSLVFRRGGGRG 263
           RG    +++    D   + P   +  D     +  FADL EL   SL      G G
Sbjct: 360 RGDDMELENVDDDDDNQIAPYRPELHDHQHQPDDFFADLEELEGDSLSMLLSHGCG 394

[Close]

BLAST of Bhi08G001772 vs. ExPASy Swiss-Prot
Match: O64747 (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.0e-26
Identity = 73/139 (52.52%), Postives = 84/139 (60.43%), Query Frame = 0


Query: 32  SPPRNSGAATATKKGRRGMKKKVVSVKINSDSPRNSSGSAPPPSDSWAWRKYGQKPIKGS 91
           S PRN G      K R+   KKVV +   +     SSG    PSD WAWRKYGQKPIKGS
Sbjct: 178 SSPRNLGI-----KRRKSQAKKVVCIPAPAAMNSRSSGEV-VPSDLWAWRKYGQKPIKGS 237

Query: 92  PYPRAYYRCSSSKGCPARKQVEKNRLDPTTLVITYSCEHNHSGPVSRN-----------N 151
           PYPR YYRCSSSKGC ARKQVE++R DP  LVITY+ EHNH  P  RN           +
Sbjct: 238 PYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSS 297

Query: 152 NNNNNNHNQTAAAALKPGS 160
           + N ++ + TAAA   P S
Sbjct: 298 SLNPSSKSSTAAATTSPSS 310

[Close]

BLAST of Bhi08G001772 vs. ExPASy Swiss-Prot
Match: O04609 (WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 5.8e-22
Identity = 67/175 (38.29%), Postives = 87/175 (49.71%), Query Frame = 0


Query: 37  SGAATATKKGRRGMKKKVVSVKINSDSPRNSSGSAPPPSDSWAWRKYGQKPIKGSPYPRA 96
           SG+ T+  K R+   KKV  V   + +           SD WAWRKYGQKPIKGSPYPR 
Sbjct: 101 SGSNTSRSKRRKIQHKKVCHVAAEALN-----------SDVWAWRKYGQKPIKGSPYPRG 160

Query: 97  YYRCSSSKGCPARKQVEKNRLDPTTLVITYSCEHNHSGPVSRNNNNNNNNHNQTAAAALK 156
           YYRCS+SKGC ARKQVE+NR DP   ++TY+ EHNH  P  RN+   +     +     K
Sbjct: 161 YYRCSTSKGCLARKQVERNRSDPKMFIVTYTAEHNHPAPTHRNSLAGSTRQKPSDQQTSK 220

Query: 157 PGSPETVPVHQEPEVEEKFVEIVGEESFITGDEFSWFGEMETTSSTVLESSIFSG 212
             +         P        +  E+    GD      ++ + S TV+    F G
Sbjct: 221 SPTTTIATYSSSPVTSADEFVLPVEDHLAVGD-LDGEEDLLSLSDTVVSDDFFDG 263

[Close]

BLAST of Bhi08G001772 vs. ExPASy TrEMBL
Match: A0A5D3BED3 (Putative WRKY transcription factor 65 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold34124G00010 PE=4 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.3e-138
Identity = 254/285 (89.12%), Postives = 266/285 (93.33%), Query Frame = 0


Query: 1   MDDVSFNNNNNHQFYSADLFEDSDELKPTPDSP-PRNSGAATATKKGRRGMKKKVVSVKI 60
           MDDVSFN+NN+HQFYS+D FEDSDELKPTPDSP P ++ AA ATKKGRRGMKKK++SVKI
Sbjct: 1   MDDVSFNHNNHHQFYSSDPFEDSDELKPTPDSPAPSSTIAAAATKKGRRGMKKKIISVKI 60

Query: 61  NSDSPRNSSGSAPPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVEKNRLDP 120
           N DSPRNSSGSA PPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVE+NRLDP
Sbjct: 61  NGDSPRNSSGSATPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP 120

Query: 121 TTLVITYSCEHNHSGPVSRNNNNNNNNHNQTAAAALKPGSPETVPVHQEPEVEEKFVEIV 180
           TTLVITYSCEHNHSGPVSRNNNNNNN +NQ     +KPGSPETV VHQEPEVEEKFVEI 
Sbjct: 121 TTLVITYSCEHNHSGPVSRNNNNNNNQNNQ--IVLMKPGSPETVAVHQEPEVEEKFVEIG 180

Query: 181 GEESFITGDEFSWFGEMETTSSTVLESSIFSGRGSSGLVDHSISSDVAMLFPMGDDDVDE 240
           GEES IT DEFSWFGEMETTSSTVLESSIFSGR S+GLVDHSISSDVAMLFPMGDDDVDE
Sbjct: 181 GEESLITADEFSWFGEMETTSSTVLESSIFSGRASTGLVDHSISSDVAMLFPMGDDDVDE 240

Query: 241 SLFADLGELPECSLVFRRGGGRGLPLDEQPAATQRRITPWCGTTT 285
           SLFADLGELPECSLVFRRGGGRGLP+DEQPAA QRRITPWCGTTT
Sbjct: 241 SLFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 283

[Close]

BLAST of Bhi08G001772 vs. ExPASy TrEMBL
Match: A0A1S3B8W7 (probable WRKY transcription factor 65 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487065 PE=4 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.3e-138
Identity = 254/285 (89.12%), Postives = 266/285 (93.33%), Query Frame = 0


Query: 1   MDDVSFNNNNNHQFYSADLFEDSDELKPTPDSP-PRNSGAATATKKGRRGMKKKVVSVKI 60
           MDDVSFN+NN+HQFYS+D FEDSDELKPTPDSP P ++ AA ATKKGRRGMKKK++SVKI
Sbjct: 1   MDDVSFNHNNHHQFYSSDPFEDSDELKPTPDSPAPSSTIAAAATKKGRRGMKKKIISVKI 60

Query: 61  NSDSPRNSSGSAPPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVEKNRLDP 120
           N DSPRNSSGSA PPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVE+NRLDP
Sbjct: 61  NGDSPRNSSGSATPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP 120

Query: 121 TTLVITYSCEHNHSGPVSRNNNNNNNNHNQTAAAALKPGSPETVPVHQEPEVEEKFVEIV 180
           TTLVITYSCEHNHSGPVSRNNNNNNN +NQ     +KPGSPETV VHQEPEVEEKFVEI 
Sbjct: 121 TTLVITYSCEHNHSGPVSRNNNNNNNQNNQ--IVLMKPGSPETVAVHQEPEVEEKFVEIG 180

Query: 181 GEESFITGDEFSWFGEMETTSSTVLESSIFSGRGSSGLVDHSISSDVAMLFPMGDDDVDE 240
           GEES IT DEFSWFGEMETTSSTVLESSIFSGR S+GLVDHSISSDVAMLFPMGDDDVDE
Sbjct: 181 GEESLITADEFSWFGEMETTSSTVLESSIFSGRASTGLVDHSISSDVAMLFPMGDDDVDE 240

Query: 241 SLFADLGELPECSLVFRRGGGRGLPLDEQPAATQRRITPWCGTTT 285
           SLFADLGELPECSLVFRRGGGRGLP+DEQPAA QRRITPWCGTTT
Sbjct: 241 SLFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 283

[Close]

BLAST of Bhi08G001772 vs. ExPASy TrEMBL
Match: E7CEW0 (WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY6 PE=2 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.9e-137
Identity = 255/287 (88.85%), Postives = 264/287 (91.99%), Query Frame = 0


Query: 1   MDDVSFNNNNNHQFYSADLFEDSDELKPTPDSPPRNS--GAATATKKGRRGMKKKVVSVK 60
           MDDVSFN+NN+HQFYS+D FEDSDELKPTPDSPP  S   AA  TKKGRRGMKKK+VSVK
Sbjct: 1   MDDVSFNHNNHHQFYSSDPFEDSDELKPTPDSPPPTSTIAAAATTKKGRRGMKKKIVSVK 60

Query: 61  INSDSPRNSSGSAPPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVEKNRLD 120
           IN DSPRNSSGSA PPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVE+NRLD
Sbjct: 61  INGDSPRNSSGSATPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLD 120

Query: 121 PTTLVITYSCEHNHSGPVSRNNNNNNNNHNQTAAAALKPGSPETVPVHQEPEVEEKFVEI 180
           PTTLVITYSCEHNHSGPVSRNNNNNNN +NQ     +KPGSPETV VHQEPEVEEKFVEI
Sbjct: 121 PTTLVITYSCEHNHSGPVSRNNNNNNNQNNQ--IVVMKPGSPETVAVHQEPEVEEKFVEI 180

Query: 181 -VGEESFITGDEFSWFGEMETTSSTVLESSIFSGRGSSGLVDHSISSDVAMLFPMGDDDV 240
             GEES IT DEFSWFGEMETTSSTVLESSIFSGR S+GLVDHSISSDVAMLFPMGDDDV
Sbjct: 181 GGGEESLITADEFSWFGEMETTSSTVLESSIFSGRASTGLVDHSISSDVAMLFPMGDDDV 240

Query: 241 DESLFADLGELPECSLVFRRGGGRGLPLDEQPAATQRRITPWCGTTT 285
           DESLFADLGELPECSLVFRRGGGRGLP+DEQPAA QRRITPWCGTTT
Sbjct: 241 DESLFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 285

[Close]

BLAST of Bhi08G001772 vs. ExPASy TrEMBL
Match: A0A1S3B8Z0 (probable WRKY transcription factor 65 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103487065 PE=4 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 1.3e-136
Identity = 253/285 (88.77%), Postives = 265/285 (92.98%), Query Frame = 0


Query: 1   MDDVSFNNNNNHQFYSADLFEDSDELKPTPDSP-PRNSGAATATKKGRRGMKKKVVSVKI 60
           MDDVSFN+NN+HQFYS+D FEDSDELKPTPDSP P ++ AA ATKKG RGMKKK++SVKI
Sbjct: 1   MDDVSFNHNNHHQFYSSDPFEDSDELKPTPDSPAPSSTIAAAATKKG-RGMKKKIISVKI 60

Query: 61  NSDSPRNSSGSAPPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVEKNRLDP 120
           N DSPRNSSGSA PPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVE+NRLDP
Sbjct: 61  NGDSPRNSSGSATPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP 120

Query: 121 TTLVITYSCEHNHSGPVSRNNNNNNNNHNQTAAAALKPGSPETVPVHQEPEVEEKFVEIV 180
           TTLVITYSCEHNHSGPVSRNNNNNNN +NQ     +KPGSPETV VHQEPEVEEKFVEI 
Sbjct: 121 TTLVITYSCEHNHSGPVSRNNNNNNNQNNQ--IVLMKPGSPETVAVHQEPEVEEKFVEIG 180

Query: 181 GEESFITGDEFSWFGEMETTSSTVLESSIFSGRGSSGLVDHSISSDVAMLFPMGDDDVDE 240
           GEES IT DEFSWFGEMETTSSTVLESSIFSGR S+GLVDHSISSDVAMLFPMGDDDVDE
Sbjct: 181 GEESLITADEFSWFGEMETTSSTVLESSIFSGRASTGLVDHSISSDVAMLFPMGDDDVDE 240

Query: 241 SLFADLGELPECSLVFRRGGGRGLPLDEQPAATQRRITPWCGTTT 285
           SLFADLGELPECSLVFRRGGGRGLP+DEQPAA QRRITPWCGTTT
Sbjct: 241 SLFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 282

[Close]

BLAST of Bhi08G001772 vs. ExPASy TrEMBL
Match: A0A6J1EGX5 (probable WRKY transcription factor 65 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434096 PE=4 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 1.1e-113
Identity = 224/288 (77.78%), Postives = 238/288 (82.64%), Query Frame = 0


Query: 1   MDDVSFNNNNNHQFYSADLFEDSDELKPTPDSPPRNSGA----ATATKKGRRGMKKKVVS 60
           MDDV+F  NNNHQFYS DL EDSDE K  PDSPP         +TA KKGRRGMKKKVVS
Sbjct: 1   MDDVTF--NNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPLPPNSTAAKKGRRGMKKKVVS 60

Query: 61  VKINSDSPRNSSGSAPPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVEKNR 120
           V+IN D PRNSSGS  PPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVE+NR
Sbjct: 61  VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNR 120

Query: 121 LDPTTLVITYSCEHNHSGPVSRNNNNNNNNHNQTAAAALKPGSPETVPVHQEPEVEEKFV 180
           LDPT L+ITYSCEHNHSGPVSR     NNNH+Q  AAA+KP SPETVPVH EPE EEKFV
Sbjct: 121 LDPTMLLITYSCEHNHSGPVSR-----NNNHHQ--AAAVKPTSPETVPVHPEPEAEEKFV 180

Query: 181 EIVGEESFITGDEFSWFGEMETTSSTVLESSIFSGRGSSGLVDHSISSDVAMLFPMGDDD 240
           E +        D+FSWFGEMETTSSTVLESSIFSGR SSG+ D SISSDVAM FPMGDDD
Sbjct: 181 ESL-------EDQFSWFGEMETTSSTVLESSIFSGRSSSGVGD-SISSDVAMPFPMGDDD 240

Query: 241 VDESLFADLGELPECSLVFRRGGGRGLPLDEQPAATQRRITPWCGTTT 285
           VD+SLFADLGELPECS+VFR GGG  LP+DEQPA +QRRITPWCG TT
Sbjct: 241 VDDSLFADLGELPECSVVFRHGGGPELPVDEQPATSQRRITPWCGATT 271

The following BLAST results are available for this feature:

BLAST of Bhi08G001772 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G29280.1	8.8e-50	49.80	WRKY DNA-binding protein 65	[more]
AT3G58710.1	5.2e-42	42.01	WRKY DNA-binding protein 69	[more]
AT3G58710.2	2.6e-41	40.89	WRKY DNA-binding protein 69	[more]
AT1G30650.1	4.2e-28	38.14	WRKY DNA-binding protein 14	[more]
AT2G34830.1	7.2e-28	52.52	WRKY DNA-binding protein 35	[more]

BLAST of Bhi08G001772 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LP56	1.2e-48	49.80	Probable WRKY transcription factor 65 OS=Arabidopsis thaliana OX=3702 GN=WRKY65 ...	[more]
Q93WV5	3.6e-40	40.89	Probable WRKY transcription factor 69 OS=Arabidopsis thaliana OX=3702 GN=WRKY69 ...	[more]
Q9SA80	6.0e-27	38.14	Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 ...	[more]
O64747	1.0e-26	52.52	Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 ...	[more]
O04609	5.8e-22	38.29	WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1	[more]

BLAST of Bhi08G001772 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BED3	1.3e-138	89.12	Putative WRKY transcription factor 65 isoform X1 OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3B8W7	1.3e-138	89.12	probable WRKY transcription factor 65 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
E7CEW0	1.9e-137	88.85	WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY6 PE=2 SV=1	[more]
A0A1S3B8Z0	1.3e-136	88.77	probable WRKY transcription factor 65 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A6J1EGX5	1.1e-113	77.78	probable WRKY transcription factor 65 isoform X1 OS=Cucurbita moschata OX=3662 G...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 74..134 e-value: 8.4E-33 score: 125.0
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 76..132 e-value: 4.7E-24 score: 84.4
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 75..135 score: 30.813437
IPR036576	WRKY domain superfamily	GENE3D	2.20.25.80	WRKY domain	coord: 60..136 e-value: 8.7E-29 score: 101.1
IPR036576	WRKY domain superfamily	SUPERFAMILY	118290	WRKY DNA-binding domain	coord: 72..134
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 131..167
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..82
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 59..74
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..17
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 131..155
None	No IPR available	PANTHER	PTHR32096:SF19	OS01G0750100 PROTEIN	coord: 29..282
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR32096	WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATED	coord: 29..282

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi08M001772	Bhi08M001772	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0000976	transcription cis-regulatory region binding
molecular_function	GO:0043565	sequence-specific DNA binding