Bhi08G001578 (gene) Wax gourd (B227) v1

BLAST of Bhi08G001578 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 1.4e-163
Identity = 301/599 (50.25%), Postives = 393/599 (65.61%), Query Frame = 0

Query: 13  PSATTTTTAGGGAS--FGPFCRNKKVPSSTIHKTNKLV--ITCSGSNGNANGEKSNNSLW 72
           P   TT T    A+    PF +N    S            ++C  +N + N +  +    
Sbjct: 6   PPVVTTPTVPNPATKPLSPFSQNNSQVSLLTKPKRSFARKVSCKATNNDQNDQAQSK--- 65

Query: 73  PGKFIDRREALIGLGGLYGTASSTFGADPFALADPVETPDPAKCHVIKNLTKDIKELNCC 132
               +DRR  L+GLGGLYG A    G DPFA A P+  PD +KC    +L +     NCC
Sbjct: 66  ----LDRRNVLLGLGGLYGVAG--MGTDPFAFAKPIAPPDVSKCGP-ADLPQGAVPTNCC 125

Query: 133 PPSTDNVKPF-----LKLRVRKAAQSLDKEYIEKYKEAVALMRALPDDDPRSFKQQADVH 192
           PP +  +  F      KLR+R  A ++D+ Y +KY +A+ LM+ALPDDDPRSFKQQA VH
Sbjct: 126 PPPSTKIIDFKLPAPAKLRIRPPAHAVDQAYRDKYYKAMELMKALPDDDPRSFKQQAAVH 185

Query: 193 CAYCNGGYNQLGF-SVELQVHFSWLFFPFHRYYLYFHERILGSLIKDPDFALPFWNYDAP 252
           CAYC+G Y+Q+GF  +ELQ+H SWLFFPFHRYYLYF E+ILG LI DP FALPFWN+D+P
Sbjct: 186 CAYCDGAYDQVGFPELELQIHNSWLFFPFHRYYLYFFEKILGKLINDPTFALPFWNWDSP 245

Query: 253 EGMEIPKIFTDESSSLYDAFRDGLHQPPTLVVLDYNKIDPDVPRQTQIDNNLTIMHRQVV 312
            GM +P I+ D  S LYD  R   HQPPTLV LDYN  + +V ++T I+ NL IM+RQ+V
Sbjct: 246 AGMPLPAIYADPKSPLYDKLRSANHQPPTLVDLDYNGTEDNVSKETTINANLKIMYRQMV 305

Query: 313 SGATTPLLFFGQPYRSGKQPNPGMGTVENIPHTPIHVWTGDRNQSSGIDMGAFFSAARDP 372
           S +    LFFG PYR+G +P+PG G++E  PH P+H+WTGD  Q +  DMG F+SA RDP
Sbjct: 306 SNSKNAKLFFGNPYRAGDEPDPGGGSIEGTPHAPVHLWTGDNTQPNFEDMGNFYSAGRDP 365

Query: 373 IFYAHHANVDRFWTIWKTLGGKRRDIKDTDFLNASFIFYDENCEAVRVYVKDCLDTRALG 432
           IF+AHH+NVDR W+IWKTLGGKR D+ D+D+L++ F+FY+EN E VRV V+DCL+T+ LG
Sbjct: 366 IFFAHHSNVDRMWSIWKTLGGKRTDLTDSDWLDSGFLFYNENAELVRVKVRDCLETKNLG 425

Query: 433 YVYDDTVKIPWLETPPTPRVPR--KTKKLKKKSVGQMG-----------KLPSVLDKIVR 492
           YVY D V IPWL + PTPR  +   +K  KK  V               + P  L   + 
Sbjct: 426 YVYQD-VDIPWLSSKPTPRRAKVALSKVAKKLGVAHAAVASSSKVVAGTEFPISLGSKIS 485

Query: 493 FEVKRPK-KSRSKKEKEDEEEILVIDGIEFDGNKCIKFDVFINDEDDTEIRANNSEFAGS 552
             VKRPK K RSKK KEDEEEILVI+GIEFD +  +KFDV++ND DD     + +EFAGS
Sbjct: 486 TVVKRPKQKKRSKKAKEDEEEILVIEGIEFDRDVAVKFDVYVNDVDDLPSGPDKTEFAGS 545

Query: 553 FVNLPHAKGSSMKINTSLRLGISKVLEDLNADDDDNIIVTLVPRFGDEPPIIQDIRIEY 588
           FV++PH+     K+NT LRLG++ +LE++ A+DDD+++VTLVP+FG     I  I+IE+
Sbjct: 546 FVSVPHSHKHKKKMNTILRLGLTDLLEEIEAEDDDSVVVTLVPKFGAVK--IGGIKIEF 591

BLAST of Bhi08G001578 vs. ExPASy Swiss-Prot
Match: O81103 (Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PPO PE=1 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 1.9e-160
Identity = 308/607 (50.74%), Postives = 393/607 (64.74%), Query Frame = 0

Query: 1   MASLSTPMPLALPSATTTTTAGGGASFGPFCRNKKVPS----STIHKTNKLVITCSGSN- 60
           MA+  +P  +   S+  +T      SF  F  NK   S    S  +   +  I+C  +N 
Sbjct: 1   MATAPSPTTMGTYSSLISTN-----SFSTFLPNKSQLSLSGKSKHYVARRSSISCKATNN 60

Query: 61  ---GNANGEKSNNSLWPGKFIDRREALIGLGGLYGTASSTFGADPFALADPVETPDPAKC 120
               N N ++  +S   GK +DRR  LIGLGGLYG  ++T    PFA ADP+  PD   C
Sbjct: 61  NNSNNQNEQQEESSRLLGK-LDRRNILIGLGGLYG--ATTLDRKPFAFADPIAPPDLTTC 120

Query: 121 HVIKNLTKDIKELNCCPPSTDNVKPF-----LKLRVRKAAQSLDKEYIEKYKEAVALMRA 180
              +      + + CCPP T  +K F     + LR   AA  +  EY+ K+K+A A MRA
Sbjct: 121 KPAEITPGGSETVPCCPPVTTKIKTFKPDLSIPLRTSPAAHQVTDEYLAKFKKAQAAMRA 180

Query: 181 LPDDDPRSFKQQADVHCAYCNGGYNQLGFS-VELQVHFSWLFFPFHRYYLYFHERILGSL 240
           LPDDDPRS  QQA VHCAYCNG Y Q+GF+  ++QVHFSWLFFPFHR YLYF+ERILG L
Sbjct: 181 LPDDDPRSMVQQAKVHCAYCNGAYPQVGFTDNDIQVHFSWLFFPFHRMYLYFYERILGKL 240

Query: 241 IKDPDFALPFWNYDAPEGMEIPKIFTDESSSLYDAFRDGLHQPPTLVVLDYNKIDPDVPR 300
           I DP FALP+WN+D+P G  IP I+TD SS LYD +R+  HQPP LV L Y   D DV  
Sbjct: 241 IDDPTFALPYWNWDSPVGFPIPDIYTDTSSPLYDQYRNADHQPPVLVDLSYGGKDDDVDE 300

Query: 301 QTQIDNNLTIMHRQVVSGATTPLLFFGQPYRSGKQPNPGM--GTVENIPHTPIHVWTGDR 360
           QT+ID NL IM+RQ+VSGA TP LFFG  YR+G   N G   GT+EN+PH  IH+W GD 
Sbjct: 301 QTRIDENLAIMYRQMVSGAKTPDLFFGHAYRAGNL-NTGKYPGTIENMPHNNIHIWVGDP 360

Query: 361 NQSSGIDMGAFFSAARDPIFYAHHANVDRFWTIWKTLGGKRRDIKDTDFLNASFIFYDEN 420
           +Q+   DMG F+SA RDP+FYAHHANVDR W IWKTLGGKR+DI DTD+L+A F+FYDEN
Sbjct: 361 SQTHQEDMGNFYSAGRDPLFYAHHANVDRMWNIWKTLGGKRKDITDTDWLDAEFLFYDEN 420

Query: 421 CEAVRVYVKDCLD-TRALGYVYDDTVKIPWLETPPTPRVPRKTKKLKKKSVGQMGKLPSV 480
            E VRV V+D L+  + L Y Y + V +PWL T PT R  +   K K  +     K P+ 
Sbjct: 421 AELVRVKVRDSLEPEKQLRYNY-EPVSLPWLFTKPTARKTKNKTKAKVAATQLTSKFPAT 480

Query: 481 LDKIVRFEVKRPK-KSRSKKEKEDEEEILVIDGIEFDGNKCIKFDVFINDEDDTEIRANN 540
           L ++   EV RPK + RSKKEK DEEE+L+I  IEF+G + +KFDVFIND+ ++  R + 
Sbjct: 481 LVEVTTVEVARPKPRKRSKKEKVDEEELLIIKDIEFEGTEAVKFDVFINDDAESLSRRDK 540

Query: 541 SEFAGSFVNLPHAKGSSMKINTSLRLGISKVLEDLNADDDDNIIVTLVPRFGDEPPIIQD 590
           SEFAGSFV++P  K +  K  T+L+LGI+ +LEDL A+DD +++VTLVPR  + P  I  
Sbjct: 541 SEFAGSFVHVPQGKTTKAKTKTNLKLGITDLLEDLGAEDDSSVLVTLVPRVSNSPITIGG 597

BLAST of Bhi08G001578 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 1.2e-159
Identity = 307/593 (51.77%), Postives = 392/593 (66.10%), Query Frame = 0

Query: 25  ASFGPFCRNKKVPSSTIHKTNKLVITCSGSNGNANGEKSNNSLWPGKFIDRREALIGLGG 84
           +S  PF + K+  SS + K  K  +TCS SN N N  K    L       RR  LIGLGG
Sbjct: 24  SSVYPFLQ-KQHQSSKLRKP-KRQVTCS-SNNNQNNPKEEQEL-SNIVGHRRNVLIGLGG 83

Query: 85  LYGTASSTFGADPFALADPVETPDPAKCHVIKNLTKDI--KELNCCPPSTDNVKPF---- 144
           +YGT ++    +P ALA P+  PD +KC    +L        +NCCPP +  +  F    
Sbjct: 84  IYGTLAT----NPSALASPISPPDLSKCVPPSDLPSGTTPPNINCCPPYSTKITDFKFPS 143

Query: 145 -LKLRVRKAAQSLDKEYIEKYKEAVALMRALPDDDPRSFKQQADVHCAYCNGGYNQLGF- 204
              LRVR+AA  +D E++EKYK+A  LM+ALP +DPR+F QQA++HCAYC+G Y+Q+GF 
Sbjct: 144 NQPLRVRQAAHLVDNEFLEKYKKATELMKALPSNDPRNFTQQANIHCAYCDGAYSQIGFP 203

Query: 205 SVELQVHFSWLFFPFHRYYLYFHERILGSLIKDPDFALPFWNYDAPEGMEIPKIFTDESS 264
            ++LQVH SWLFFPFHR+YLYF+ERILGSLI DP FALPFWNYDAP+GM++P I+ D++S
Sbjct: 204 DLKLQVHGSWLFFPFHRWYLYFYERILGSLINDPTFALPFWNYDAPDGMQLPTIYADKAS 263

Query: 265 SLYDAFRDGLHQPPTLVVLDYNKIDPDVPRQTQIDNNLTIMHRQVVSGATTPLLFFGQPY 324
            LYD  R+  HQPPTL+ L++  I  D+ R   I  NL+IM+RQV S   T  LF G PY
Sbjct: 264 PLYDELRNASHQPPTLIDLNFCDIGSDIDRNELIKTNLSIMYRQVYSNGKTSRLFLGNPY 323

Query: 325 RSGKQPNPGMGTVENIPHTPIHVWTGDRNQSSGIDMGAFFSAARDPIFYAHHANVDRFWT 384
           R+G     G G++EN+PH P+H WTGD  Q++  DMG F+SAARDPIFY+HH+NVDR W 
Sbjct: 324 RAGDAEPQGAGSIENVPHAPVHTWTGDNTQTNIEDMGIFYSAARDPIFYSHHSNVDRLWY 383

Query: 385 IWKTLGGKRRDIKDTDFLNASFIFYDENCEAVRVYVKDCLDTRALGYVYDDTVKIPWLET 444
           IWKTLGGK+ D  D D+L + F+FYDEN   VRV VKD LD   LGY Y D V IPW + 
Sbjct: 384 IWKTLGGKKHDFTDKDWLESGFLFYDENKNLVRVNVKDSLDIDKLGYAYQD-VPIPWEKA 443

Query: 445 PPTPRVPRKTKKLKKKSV--GQMGKLPS-----------------VLDKIVRFEVKRPKK 504
            P PR   K +KL +  V  G + K P+                 VL+  V   VKRPKK
Sbjct: 444 KPVPR-RTKVQKLVEVEVNDGNLRKSPTIFLVRQQSPRKYVTFPLVLNNKVSAIVKRPKK 503

Query: 505 SRSKKEKEDEEEILVIDGIEFDGNKCIKFDVFINDEDDTEIRANNSEFAGSFVNLPHAK- 564
            RSKKEKE+EEE+LVI+GIEF  N  IKFDV+INDEDD ++ A N+EFAGSFVN+PH+  
Sbjct: 504 LRSKKEKEEEEEVLVIEGIEFYMNIAIKFDVYINDEDD-KVGAGNTEFAGSFVNIPHSAH 563

Query: 565 -GSSMKINTSLRLGISKVLEDLNADDDDNIIVTLVPRFGDEPPIIQDIRIEYD 589
              + KI TSLRLGI+ +LEDL+ + DDNI+VTLVP+ G     I ++ I ++
Sbjct: 564 GHKNKKIITSLRLGITDLLEDLHVEGDDNIVVTLVPKCGSGQVKINNVEIVFE 605

BLAST of Bhi08G001578 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 2.6e-157
Identity = 289/606 (47.69%), Postives = 391/606 (64.52%), Query Frame = 0

Query: 8   MPLALPSATTTTTAGGGASFGP---FCRNKKVPSSTIHKTNKLV---ITCSGSNGNANGE 67
           +P +L ++T        ++F P   F R   VP +  +++ +     ++C+ +NG+ N +
Sbjct: 4   LPWSLTTSTAIANTTNISAFPPSPLFQRASHVPVAR-NRSRRFAPSKVSCNSANGDPNSD 63

Query: 68  KSNN--SLWPGKFIDRREALIGLGGLYGTASSTFGADPFALADPVETPDPAKCHVIKNLT 127
            +++      GK +DRR  L+G+GGLYG A       P A   P++ PD +KC     + 
Sbjct: 64  STSDVRETSSGK-LDRRNVLLGIGGLYGAAGGLGATKPLAFGAPIQAPDISKCGT-ATVP 123

Query: 128 KDIKELNCCPPSTDNVKPF------LKLRVRKAAQSLDKEYIEKYKEAVALMRALPDDDP 187
             +   NCCPP T  +  F        +R R AA  + KEY+ KYK+A+ L +ALPDDDP
Sbjct: 124 DGVTPTNCCPPVTTKIIDFQLPSSGSPMRTRPAAHLVSKEYLAKYKKAIELQKALPDDDP 183

Query: 188 RSFKQQADVHCAYCNGGYNQLGFS-VELQVHFSWLFFPFHRYYLYFHERILGSLIKDPDF 247
           RSFKQQA+VHC YC G Y+Q+G++ +ELQVH SWLF PFHRYYLYF+ERIL  LI DP F
Sbjct: 184 RSFKQQANVHCTYCQGAYDQVGYTDLELQVHASWLFLPFHRYYLYFNERILAKLIDDPTF 243

Query: 248 ALPFWNYDAPEGMEIPKIFTDESSSLYDAFRDGLHQPPTLVVLDYNKIDPDVPRQTQIDN 307
           ALP+W +D P+GM +P I+    SSLYD  R+  H PPT++ LDY+  +P +P      +
Sbjct: 244 ALPYWAWDNPDGMYMPTIYASSPSSLYDEKRNAKHLPPTVIDLDYDGTEPTIPDDELKTD 303

Query: 308 NLTIMHRQVVSGATTPLLFFGQPYRSGKQPNPGMGTVENIPHTPIHVWTGDRNQSSGIDM 367
           NL IM++Q+VSGATTP LF G PYR+G   +PG GT+E+ PH  +H WTG  ++ S  DM
Sbjct: 304 NLAIMYKQIVSGATTPKLFLGYPYRAGDAIDPGAGTLEHAPHNIVHKWTGLADKPSE-DM 363

Query: 368 GAFFSAARDPIFYAHHANVDRFWTIWKTLGGK-RRDIKDTDFLNASFIFYDENCEAVRVY 427
           G F++A RDPIF+ HHANVDR W IWKT+GGK R+D  DTD+L+A+F+FYDEN + V+V 
Sbjct: 364 GNFYTAGRDPIFFGHHANVDRMWNIWKTIGGKNRKDFTDTDWLDATFVFYDENKQLVKVK 423

Query: 428 VKDCLDTRALGYVYDDTVKIPWLETPPTPRVPRKTK----------KLKKKSVGQMGKLP 487
           V DC+DT  L Y Y D + IPWL      +    TK          +L K ++  +G  P
Sbjct: 424 VSDCVDTSKLRYQYQD-IPIPWLPKNTKAKAKTTTKSSKSGVAKAAELPKTTISSIGDFP 483

Query: 488 SVLDKIVRFEVKRPKKSRSKKEKEDEEEILVIDGIEFDGNKCIKFDVFINDEDDTEIRAN 547
             L+ ++R EV RPKKSRSKKEKEDEEE+L+I GIE D    +KFDV+INDED +  R  
Sbjct: 484 KALNSVIRVEVPRPKKSRSKKEKEDEEEVLLIKGIELDRENFVKFDVYINDEDYSVSRPK 543

Query: 548 NSEFAGSFVNLPHAKGSSMKINTSLRLGISKVLEDLNADDDDNIIVTLVPRFGDEPPIIQ 588
           NSEFAGSFVN+PH     MK  T+LR  I+++LEDL A+DD+++IVT+VPR G +   I 
Sbjct: 544 NSEFAGSFVNVPHKHMKEMKTKTNLRFAINELLEDLGAEDDESVIVTIVPRAGGDDVTIG 603

BLAST of Bhi08G001578 vs. ExPASy Swiss-Prot
Match: Q9FRX6 (Aureusidin synthase OS=Antirrhinum majus OX=4151 GN=AS1 PE=1 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.7e-148
Identity = 280/521 (53.74%), Postives = 357/521 (68.52%), Query Frame = 0

Query: 84  GLYGTASSTFGADPFALADPVETPDPAKCHVIKNLTKDIKELNCCPPSTDNV------KP 143
           GLY   S  +         P+  PD  KC    +L      +NCCPP    +       P
Sbjct: 43  GLYIANSLAYARFASTSTGPIAAPDVTKCGQ-PDLPPGTAPINCCPPIPAKIIDFELPPP 102

Query: 144 FLKLRVRKAAQSLDKEYIEKYKEAVALMRALPDDDPRSFKQQADVHCAYCNGGYNQLGF- 203
              +RVR+AA  +D  YI K+K+AV LMRALP+DDPRSFKQQA+VHCAYC G YNQ GF 
Sbjct: 103 STTMRVRRAAHLVDDAYIAKFKKAVELMRALPEDDPRSFKQQANVHCAYCAGAYNQAGFT 162

Query: 204 SVELQVHFSWLFFPFHRYYLYFHERILGSLIKDPDFALPFWNYDAPEGMEIPKIFTDESS 263
           +++LQ+H SWLFFPFHRYY+YF ERILG LI D  FALPFWNYD+P GM IP +F D +S
Sbjct: 163 NLKLQIHRSWLFFPFHRYYIYFFERILGKLINDTTFALPFWNYDSPGGMTIPSMFIDTNS 222

Query: 264 SLYDAFRDGLHQPPTLVVLDYNKIDPD--VPRQTQIDNNLTIMHRQVVSGATTPLLFFGQ 323
           SLYD+ RD  HQPPT+V L+Y   D D     + Q+  NL I++RQ+VS A TP LFFG+
Sbjct: 223 SLYDSLRDSNHQPPTIVDLNYAFSDSDNTTTPEEQMIINLKIVYRQMVSSAKTPQLFFGR 282

Query: 324 PYRSGKQPNPGMGTVENIPHTPIHVWTGDRNQSSGIDMGAFFSAARDPIFYAHHANVDRF 383
           PYR G Q  PG+G++E +PH  IH+WTG  N   G +MGAF+S ARDPIF+AHH+NVDR 
Sbjct: 283 PYRRGDQEFPGVGSIELVPHGMIHLWTGSENTPYGENMGAFYSTARDPIFFAHHSNVDRM 342

Query: 384 WTIWKTLGGKRR-DIKDTDFLNASFIFYDENCEAVRVYVKDCLDTRALGYVYDDTVKIPW 443
           W+IWKTLGG RR D+ D DFL+ASF+FYDEN E VRV V+DCLD + LGYVY D V+IPW
Sbjct: 343 WSIWKTLGGPRRTDLTDPDFLDASFVFYDENAEMVRVKVRDCLDEKKLGYVYQD-VEIPW 402

Query: 444 LETPPTPRV-PRKTKKLKKKSVGQMGKL-PSVLDKIVRFEVKRPKKSRSKKEKEDEEEIL 503
           L T PTP+V P   KK  + +     ++ P++LD++++  V RPKK+RS+KEK++ EEIL
Sbjct: 403 LNTRPTPKVSPSLLKKFHRTNTANPRQVFPAILDRVLKVIVTRPKKTRSRKEKDELEEIL 462

Query: 504 VIDGIEFD-GNKCIKFDVFIN-DEDD-TEIRANNSEFAGSFVNLPHAKGSSMKINTS-LR 563
           VI+GIE +  +  +KFDV+IN DEDD   I   N+EFAGSFV+L H      +  T  L 
Sbjct: 463 VIEGIELERDHGHVKFDVYINADEDDLAVISPENAEFAGSFVSLWHKPIKGKRTKTQLLT 522

Query: 564 LGISKVLEDLNADDDDNIIVTLVPRFGDEPPIIQDIRIEYD 589
           L I  +LEDL+AD+DD ++VTLVPR   +   I +++IE D
Sbjct: 523 LSICDILEDLDADEDDYVLVTLVPRNAGDAIKIHNVKIELD 561

BLAST of Bhi08G001578 vs. ExPASy TrEMBL
Match: A0A1L5JHU3 (Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 2.6e-184
Identity = 341/610 (55.90%), Postives = 420/610 (68.85%), Query Frame = 0

Query: 9   PLALPSATTTTTAGGGASFGPFCRNKKVPSSTIHKTNKLV-----ITCSGSNGNANGEKS 68
           P AL SA TTTT      FGPF  N K P   + +T         I CS SNG  N    
Sbjct: 5   PPALSSAATTTT------FGPFLPNNKNPPPNLRRTKYFSSKAPRILCSASNGGQN---- 64

Query: 69  NNSLWPGKFIDRREALIGLGGLYGTASSTFGADPFALADPVETPDPAKCHVIKNLTKDIK 128
                    IDRR+ LIGLGGLYG  +ST      A A PV+TP+ +KC    +L     
Sbjct: 65  --------HIDRRDILIGLGGLYG--ASTLSQGSLAFAAPVQTPNISKCGQ-PDLPPGAD 124

Query: 129 ELNCCPPSTDNVKPFL-----KLRVRKAAQSLDKEYIEKYKEAVALMRALPDDDPRSFKQ 188
            +NCCPP T  V  F      KLRVR AA   D+ Y+EKYK AV LM+ALPDDDPRSFKQ
Sbjct: 125 PINCCPPPTTAVVDFRPQAPGKLRVRPAAHLADQNYVEKYKRAVELMKALPDDDPRSFKQ 184

Query: 189 QADVHCAYCNGGYNQLGFSVELQVHFSWLFFPFHRYYLYFHERILGSLIKDPDFALPFWN 248
           QA+VHCAYCNG Y+Q+GF VE+QVH SWLFFPFHRYYL+FHE+ILG LI DP FALPFWN
Sbjct: 185 QANVHCAYCNGAYDQVGFPVEVQVHNSWLFFPFHRYYLHFHEKILGELIGDPSFALPFWN 244

Query: 249 YDAPEGMEIPKIFTDESSSLYDAFRDGLHQPPTLVVLDYNKIDPDVPRQTQIDNNLTIMH 308
           YDAP GM++P ++ DE SSLYD  R+  H PPTLV LD+  +DP +  + QI +NL+IM+
Sbjct: 245 YDAPGGMKMPAMYADEKSSLYDELRNRNHLPPTLVDLDFGGVDPTIGDEAQIRSNLSIMY 304

Query: 309 RQVVSGATTPLLFFGQPYRSGKQPNPGMGTVENIPHTPIHVWTGDRNQSSGIDMGAFFSA 368
           RQ+VS + TP LFFG  Y +G +P+PG G VENIPH P+H+W GD N+ +  +MG F+SA
Sbjct: 305 RQMVSNSRTPSLFFGNAYLAGDEPSPGGGLVENIPHGPVHIWCGDNNEPNFENMGNFYSA 364

Query: 369 ARDPIFYAHHANVDRFWTIWKTLGGKRRDIKDTDFLNASFIFYDENCEAVRVYVKDCLDT 428
           ARDPIFYAHH+N+DR W++WKTLGG+R+D+++ D+LNASFIFYDE  +AVRV V+DCLD+
Sbjct: 365 ARDPIFYAHHSNIDRLWSVWKTLGGRRQDLQNADWLNASFIFYDEKAQAVRVRVRDCLDS 424

Query: 429 RALGYVYDDTVKIPWLETPPTPRVPRKTKKL---------KKKSVGQMG----------- 488
           R LGYVY D V++PWLE+ PTPR   + +K             + G+ G           
Sbjct: 425 RNLGYVYQD-VQLPWLESRPTPRRALRAQKAVAHLPFGVGPALAAGEPGPSSSRLRPAKI 484

Query: 489 KLPSVLDKIVRFEVKRPKKSRSKKEKEDEEEILVIDGIEFDGNKCIKFDVFINDEDDTEI 548
           K P  L+  +  EVKRPK+SRSKKEK++EEEI+VI GIEFD NK IKFDVFINDEDD EI
Sbjct: 485 KFPITLNSPISMEVKRPKRSRSKKEKDEEEEIIVIYGIEFDANKAIKFDVFINDEDDKEI 544

Query: 549 RANNSEFAGSFVNLPHAKGSSMKINTSLRLGISKVLEDLNADDDDNIIVTLVPRFGDEPP 589
           R +N+EFAGSFVN+PH  G    + T LRLGI+++LEDL+ADDDD++ VTLVPR G    
Sbjct: 545 RPDNTEFAGSFVNVPHKHGHKKNVKTGLRLGITELLEDLDADDDDSVRVTLVPRSGKGLV 592

BLAST of Bhi08G001578 vs. ExPASy TrEMBL
Match: A0A6J1CGK8 (polyphenol oxidase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111010634 PE=3 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 4.2e-179
Identity = 323/574 (56.27%), Postives = 407/574 (70.91%), Query Frame = 0

Query: 41  IHKTNK-LVITCSGSNGNANGEKSNNSLWPGKFIDRREALIGLGGLYGTASSTFGADPFA 100
           ++K NK   + CS SN + +  +S     PG  +DRR+ LIGLGGLYG +S     D FA
Sbjct: 30  VNKNNKHCRVVCSASNKHDSQSQS-----PGNMVDRRDVLIGLGGLYGASSFAPEXDSFA 89

Query: 101 LADPVETPDPAKCHVIKNLTKDIKELNCCPPSTDNVKPF-----LKLRVRKAAQSLDKEY 160
           +A PV+TP   KC   +   +D K++ CCPP +  +K F      KLRVR AA  +DK Y
Sbjct: 90  IAAPVDTPQITKCGPAQ--VRD-KKVECCPPPSIKIKDFRPTPPRKLRVRPAAHLVDKNY 149

Query: 161 IEKYKEAVALMRALPDDDPRSFKQQADVHCAYCNGGYNQLGFSVELQVHFSWLFFPFHRY 220
           ++KY+ AV LMR LPD DPRSF QQA++HCAYCNGGY QLGF VELQVH SWLFFPFHR+
Sbjct: 150 LKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQVHNSWLFFPFHRF 209

Query: 221 YLYFHERILGSLIKDPDFALPFWNYDAPEGMEIPKIFTDESSSLYDAFRDGLHQPPTLVV 280
           YLYFHE+ILG LI DP FALPFWNYDAP GM  P IF +++S LYD FR+  H PPT+V 
Sbjct: 210 YLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNENHMPPTVVD 269

Query: 281 LDYNKIDPDVPRQTQIDNNLTIMHRQVVSGATTPLLFFGQPYRSGKQPNPGMGTVENIPH 340
           LDYN  D ++  + ++ +NL +M+RQ+VSG+ T  LFFG+PYR+G  P+PG G+VEN+PH
Sbjct: 270 LDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDPSPGAGSVENVPH 329

Query: 341 TPIHVWTGDRNQSSGIDMGAFFSAARDPIFYAHHANVDRFWTIWKTLGGKRRDIKDTDFL 400
            PIH W G+++Q +G DMG F+SA RDP+FY+HHANVDR W IWKTLG +RRDI++ DFL
Sbjct: 330 IPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRRRDIQNPDFL 389

Query: 401 NASFIFYDENCEAVRVYVKDCLDTRALGYVYDDTVKIPWLETPPTPRVPRKTKKLKKKSV 460
           NASF+FY+E  EAVRV V+DCLDT ALGYVY D V IPWL+T P+PR     KKL+K  V
Sbjct: 390 NASFVFYNEKKEAVRVRVRDCLDTTALGYVYQD-VDIPWLKTAPSPR----RKKLRKGGV 449

Query: 461 --GQMGKLPSV------------------LDKIVRFEVKRPKKSRSKKEKEDEEEILVID 520
               M K PS+                  L+ ++R +VKRPKKSRS KEKE+EEEILVI 
Sbjct: 450 ANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEEEEEILVIS 509

Query: 521 GIEFDGNKCIKFDVFINDEDDTEIRANNSEFAGSFVNLPHAKGSSMKINTSLRLGISKVL 580
           GIEFD N  +KFDV IN E+D EIRA+N+EFAGSF ++PH   +   + T LRLGI+++L
Sbjct: 510 GIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLRLGITELL 569

Query: 581 EDLNADDDDNIIVTLVPRFGDEPPIIQDIRIEYD 589
           EDL  +D D+++VTLVPR  D+   I  I IE+D
Sbjct: 570 EDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFD 590

BLAST of Bhi08G001578 vs. ExPASy TrEMBL
Match: A0A5N6NPH8 (Uncharacterized protein OS=Mikania micrantha OX=192012 GN=E3N88_19051 PE=3 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 8.3e-175
Identity = 323/608 (53.12%), Postives = 419/608 (68.91%), Query Frame = 0

Query: 4   LSTPMPLALPSATTTTTAGGGASFGPFC--------RNKKVPSSTIHKTNKLVITCSGSN 63
           L+    LA P  TTTTTA  G  F            ++ ++P S   K +   ++C   +
Sbjct: 2   LTMASSLASPPPTTTTTAATGKLFSSSSTYVSSFSFKSSQIPISRNRKNH--AVSCKTLD 61

Query: 64  GNANGEKSNNSLWPGKFIDRREALIGLGGLYGTASSTFGADPFALADPVETPDPAKCHVI 123
            + + E +      GK +DRR  ++GLGGLYGTA +TFG+   A ADP+  PD  KC   
Sbjct: 62  NDHHDENT------GK-LDRRNLMLGLGGLYGTA-ATFGSGSLAFADPIMAPDITKCGA- 121

Query: 124 KNLTKDIKELNCCPPSTDNVKPFLKL-----RVRKAAQSLDKEYIEKYKEAVALMRALPD 183
            +L +  K  NCCPP    +  F  L     RVR AA  ++K+YI K+ +A+ LM+ALPD
Sbjct: 122 ADLPEGAKPTNCCPPLAKKITDFKPLPPSITRVRPAAHLVNKDYIAKFNKAIELMKALPD 181

Query: 184 DDPRSFKQQADVHCAYCNGGYNQLGF-SVELQVHFSWLFFPFHRYYLYFHERILGSLIKD 243
           DDPRSFKQQA VHCAYC+G Y+Q+GF  +ELQVH SWLF PFHR+YLYF E+I G LI D
Sbjct: 182 DDPRSFKQQAAVHCAYCDGAYDQVGFPDLELQVHGSWLFLPFHRHYLYFFEKICGKLIGD 241

Query: 244 PDFALPFWNYDAPEGMEIPKIFTDESSSLYDAFRDGLHQPPTLVVLDYNKIDPDVPRQTQ 303
           P+FA+PFWN+DAP+GM+IP I+T ++S LYD+FRD  HQ P++V LD+N +D ++ R  Q
Sbjct: 242 PNFAIPFWNWDAPDGMKIPDIYTSKTSPLYDSFRDAQHQSPSIVDLDFNGVDENLSRSKQ 301

Query: 304 IDNNLTIMHRQVVSGATTPLLFFGQPYRSGKQPNPGMGTVENIPHTPIHVWTGDRNQSSG 363
           +  NLTIM+RQ+VS A T  LF G PYR+G + NPG G++E+IPH P+HVWTGDR Q +G
Sbjct: 302 VSTNLTIMYRQIVSSAKTASLFMGSPYRAGDEANPGGGSLESIPHGPVHVWTGDRTQPNG 361

Query: 364 IDMGAFFSAARDPIFYAHHANVDRFWTIWKTLGGKRRDIKDTDFLNASFIFYDENCEAVR 423
            DMG F+SAARDPIFYAHHANVDR W++WKTLGGKR D  D D+L++SF+FYDEN E VR
Sbjct: 362 EDMGNFYSAARDPIFYAHHANVDRMWSVWKTLGGKRNDFSDKDWLDSSFLFYDENAEMVR 421

Query: 424 VYVKDCLDTRALGYVYDDTVKIPWLETPPTPRVPRKTKKLKKKSVGQMGK--------LP 483
           V V+DCLD++ LGYVY + V+ PWL + PTPR+ R   K+KK  V +  +         P
Sbjct: 422 VKVRDCLDSKNLGYVYQN-VETPWLNSKPTPRIKRVLSKIKKLGVARADEHIPFAKDVFP 481

Query: 484 SVLDKIVRFEVKRPKKSRSKKEKEDEEEILVIDGIEFDGNKCIKFDVFINDEDD-TEIRA 543
           + LDK ++  V RPKKSRSKK+KE+EEEILVI GIE   +  +KFDVF+NDEDD     A
Sbjct: 482 ASLDKPIKVLVPRPKKSRSKKQKEEEEEILVIQGIEVKRDAFVKFDVFVNDEDDGMNATA 541

Query: 544 NNSEFAGSFVNLPHAKGSSMKINTSLRLGISKVLEDLNADDDDNIIVTLVPRFGDEPPII 589
           + +EFAGSFVN+PH       + T LRLGIS++LEDLNADDDDN++VTLVP+ G     I
Sbjct: 542 DKTEFAGSFVNVPHKHKHKKNVKTRLRLGISELLEDLNADDDDNVLVTLVPKNGGGEISI 597

BLAST of Bhi08G001578 vs. ExPASy TrEMBL
Match: A0A068TU86 (Uncharacterized protein OS=Coffea canephora OX=49390 GN=GSCOC_T00029229001 PE=3 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 1.2e-173
Identity = 318/603 (52.74%), Postives = 404/603 (67.00%), Query Frame = 0

Query: 1   MASLSTPMPLALPSATTTTTAGGGASFGPFCRNKKVPSSTIHKTNKLVITCSGSNGNANG 60
           MASLST       +A T+ T      + PF       S+T  + +   ++C  +NG  N 
Sbjct: 1   MASLST------STACTSATTLSSLHYSPFLAKTFHISTTKKRNHHFKVSCQATNGERNA 60

Query: 61  EKSNNSLWPGKFIDRREALIGLGGLYGTASSTFGADPFALADPVETPDPAKCHVIKNLTK 120
                         RR  LIGLGGLYG AS    ADPFALA P++ PD +KC    +L  
Sbjct: 61  -------------SRRNVLIGLGGLYGAAS--LAADPFALAAPIQAPDVSKCGP-ADLPS 120

Query: 121 DIKELNCCPPSTDNV------KPFLKLRVRKAAQSLDKEYIEKYKEAVALMRALPDDDPR 180
                NCCPP    +       P   LRVR AA  +D+ YI K++ AV LM+ALPDDDPR
Sbjct: 121 GATPTNCCPPPATTIVDFKLPAPSNTLRVRPAAHLVDEAYIAKFQRAVQLMKALPDDDPR 180

Query: 181 SFKQQADVHCAYCNGGYNQLGF-SVELQVHFSWLFFPFHRYYLYFHERILGSLIKDPDFA 240
           SFKQQA+VHCAYC+G Y+Q+GF ++ELQVH SWLF PFHRYYLYF+ERILG LI DP FA
Sbjct: 181 SFKQQANVHCAYCDGAYDQVGFPNLELQVHNSWLFLPFHRYYLYFYERILGKLINDPTFA 240

Query: 241 LPFWNYDAPEGMEIPKIFTDESSSLYDAFRDGLHQPPTLVVLDYNKIDPDVPRQTQIDNN 300
           +PFWN+DA  GM +P I+TD +S LYD  RDG HQPPT++ LD+N  D ++    Q+  N
Sbjct: 241 MPFWNWDAQGGMRLPDIYTDPTSPLYDTLRDGSHQPPTMIDLDFNGTDSNISDSQQLSQN 300

Query: 301 LTIMHRQVVSGATTPLLFFGQPYRSGKQPNPGMGTVENIPHTPIHVWTGDRNQSSGIDMG 360
            TIM+RQ+VS + TP LFFG PYR+G  P+PG G +ENIPH P+HVWTGDR Q +  DMG
Sbjct: 301 YTIMYRQMVSNSRTPRLFFGSPYRAGDDPDPGAGQLENIPHGPVHVWTGDRTQPNLEDMG 360

Query: 361 AFFSAARDPIFYAHHANVDRFWTIWKTLGGKRRDIKDTDFLNASFIFYDENCEAVRVYVK 420
            F+SA RDPIFYAHH+N+DR WT+WKTLGG+R+DI DTD+LNASF+FYDEN + VRV V+
Sbjct: 361 NFYSAGRDPIFYAHHSNIDRMWTLWKTLGGRRQDITDTDWLNASFVFYDENAQMVRVRVR 420

Query: 421 DCLDTRALGYVYDDTVKIPWLETPPTPRVPRKTKKLKKKSVGQMGK--------LPSVLD 480
           DCLDT  LGY Y  TV  PWL + PTPR+ +  +K+KK  V              P+ LD
Sbjct: 421 DCLDTTKLGYSY-QTVDTPWLNSRPTPRLTKVARKIKKLVVANAADHSHSPTDIFPAKLD 480

Query: 481 KIVRFEVKRPKKSRSKKEKEDEEEILVIDGIEFDGNKCIKFDVFINDEDDTEIRANNSEF 540
           KI++  VKRPKK RSKKEK+++EEILVI+ IE + +   KFD++INDEDD +    N+EF
Sbjct: 481 KIIKVMVKRPKKKRSKKEKDEKEEILVIEKIEVERDAFAKFDIYINDEDDPKSTPENTEF 540

Query: 541 AGSFVNLPHAKGSSMKINTSLRLGISKVLEDLNADDDDNIIVTLVPRFGDEPPIIQDIRI 589
           AGSFVN+PH      KI T L L ++++LEDL+A+DD++++VTLVPR G +   I  I+I
Sbjct: 541 AGSFVNVPHKHKHGKKIKTHLNLSLTEILEDLDAEDDEHVMVTLVPRVGCDAVTIGGIKI 580

BLAST of Bhi08G001578 vs. ExPASy TrEMBL
Match: A0A5B7BMB1 (Putative polyphenoloxidase (Fragment) OS=Davidia involucrata OX=16924 GN=Din_039573 PE=3 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 1.2e-173
Identity = 325/594 (54.71%), Postives = 405/594 (68.18%), Query Frame = 0

Query: 14  SATTTTTAGGGASFGPFCRNKKVPSSTIHKTN-KLVITCSGSNGNANGEKSNNSLWPGKF 73
           + TTTTT     S  PF        S I K N +  + C  ++ + NGE   NSL  GKF
Sbjct: 4   TTTTTTTTTSALSSRPFFSKSTSQVSIIGKRNHRFNVACKTTDSDQNGE--TNSL--GKF 63

Query: 74  IDRREALIGLGGLYGTASSTFGADPFALADPVETPDPAKCHVIKNLTKDIKELNCCPPST 133
            DRR+ L+GLGGLYG A+S   ADP A A P+  PD  KC    +L    +  NCCPP +
Sbjct: 64  -DRRKVLLGLGGLYGAATS-LSADPLAFAAPISPPDLTKCGA-ADLPTGARPTNCCPPPS 123

Query: 134 DNV------KPFLKLRVRKAAQSLDKEYIEKYKEAVALMRALPDDDPRSFKQQADVHCAY 193
             +       P   +RVR AA   DK+YI KYK AV LM+ALP DDPR+F QQA+VHCAY
Sbjct: 124 TKIVDFKLPPPSNTMRVRPAAHLADKDYIAKYKRAVELMKALPADDPRNFTQQANVHCAY 183

Query: 194 CNGGYNQLGF-SVELQVHFSWLFFPFHRYYLYFHERILGSLIKDPDFALPFWNYDAPEGM 253
           C+G Y+Q+GF  +ELQVH SWLFFPFHRYYLYFHE+ILG LI DP FALP+WN+DA   M
Sbjct: 184 CDGAYHQVGFPDLELQVHNSWLFFPFHRYYLYFHEKILGKLIGDPTFALPYWNWDA--SM 243

Query: 254 EIPKIFTDESSSLYDAFRDGLHQPPTLVVLDYNKIDPDVP--RQTQIDNNLTIMHRQVVS 313
            +P ++ D +S LYD  RD  HQPP L+ LDYN  D D P   Q QI +NL IM+RQ+ S
Sbjct: 244 PLPSMYADSNSPLYDPLRDPQHQPPRLIDLDYNPGD-DTPTSNQQQISSNLAIMYRQMTS 303

Query: 314 GATTPLLFFGQPYRSGKQPNPGMGTVENIPHTPIHVWTGDRNQSSGIDMGAFFSAARDPI 373
              TP LF G PYR+G +P+PG G++EN PH P+HVWTGDR Q +  DMG F+SAARDPI
Sbjct: 304 NGRTPRLFLGSPYRAGDEPDPGAGSIENYPHGPVHVWTGDRRQPNFEDMGNFYSAARDPI 363

Query: 374 FYAHHANVDRFWTIWKTLGGKRRDIKDTDFLNASFIFYDENCEAVRVYVKDCLDTRALGY 433
           FYAHH NVDR WTIWKTLGG+R+D  D D+L+A+F+FYDEN + VRV V+DCLD   LG+
Sbjct: 364 FYAHHTNVDRMWTIWKTLGGRRQDFTDPDWLDAAFLFYDENAQLVRVKVRDCLDNTKLGF 423

Query: 434 VYDDTVKIPWLETPPTPRVPRKTKKLKKKSVGQMGK---------LPSVLDKIVRFEVKR 493
            Y D V IPWL + PTPRV +  +K+KK  V Q  +          P+ LDK+VR  V R
Sbjct: 424 AYQD-VDIPWLNSRPTPRVAKVVRKIKKAGVAQAAETSPSTVRDVFPTSLDKVVRVVVPR 483

Query: 494 PKKSRSKKEKEDEEEILVIDGIEFDGNKCIKFDVFINDEDDTEIRANNSEFAGSFVNLPH 553
           PKKSRS+K+K++E+EIL+I+GIE + +   KFDV+INDED+  IR + +EFAGSFVN+PH
Sbjct: 484 PKKSRSRKQKDEEDEILLIEGIEVERDVYAKFDVYINDEDEPAIRPDKTEFAGSFVNVPH 543

Query: 554 AKGSSMKINTSLRLGISKVLEDLNADDDDNIIVTLVPRFGDEPPIIQDIRIEYD 589
                 KI T LRLGI+ +LEDL A+DDDN++VTLVPR+G     I  I+IE+D
Sbjct: 544 KHKHGRKIKTVLRLGITDLLEDLGAEDDDNVLVTLVPRYGTGAVTIGGIKIEFD 586