Bhi08G001542 (gene) Wax gourd (B227) v1

Overview
NameBhi08G001542
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionEF-hand domain-containing protein
Locationchr8: 54362161 .. 54362891 (+)
RNA-Seq ExpressionBhi08G001542
SyntenyBhi08G001542
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAATACACAAAACCCAATTTCCTCTCACTCTCTCATATTGATCTGTACATTGGTTGTCTTCCATAAGGCAAACTTCCCCCAATTTCCACTCTTCCATTCCCTTATTAAATACCCTCCAAATACACACACTTTCTCATCATCCATTCTTCCAATAATCCTCAAATCTATTCCCACAATATGGGATCAGGAAACGGTGTCGTTTTCTTCAAAGATTTCTTCCCTGAAATGGTGGAGAAACTGGGAATTAAAGGGTTCATGAAGGAATTGAGATCTGGGTTTTATTTGATGATGGATAAAGAAAAAGGTCTGATAACTCCAGAAAGCTTGAGAAAAAACATGGGATTTTTGGGATTAATATTGGAAGATGAAGAAGTGATGTTGATGGTGAGAGAAGGCGATTTAGATGGAGATGGAGCTTTGAATGAAGCTGAATTTTGCATTCTTATGATCAGACTTAGCCCAACTTTGTTTCACCAATCTCATTTGGATAAAAACTTTGGGAGGAAATAGACGTATTCATATTGTTTAATTTTATTTTACTCTTTTTTTTTCTCTCCCTTTTGTTACATTGTATATATTATATTAGCTAAATAATGTAAACTTTGTTTGAATATTTGAGCATTATATAATCTATCTGAGTTGTACTTGTTTTTATATTAAATGTTTGATCATATTTTTGTAATAGTGTGTATTACACGTTTTATGTCTCTAGGCATGGAGAGGGCA

mRNA sequence

AAAAAAAATACACAAAACCCAATTTCCTCTCACTCTCTCATATTGATCTGTACATTGGTTGTCTTCCATAAGGCAAACTTCCCCCAATTTCCACTCTTCCATTCCCTTATTAAATACCCTCCAAATACACACACTTTCTCATCATCCATTCTTCCAATAATCCTCAAATCTATTCCCACAATATGGGATCAGGAAACGGTGTCGTTTTCTTCAAAGATTTCTTCCCTGAAATGGTGGAGAAACTGGGAATTAAAGGGTTCATGAAGGAATTGAGATCTGGGTTTTATTTGATGATGGATAAAGAAAAAGGTCTGATAACTCCAGAAAGCTTGAGAAAAAACATGGGATTTTTGGGATTAATATTGGAAGATGAAGAAGTGATGTTGATGGTGAGAGAAGGCGATTTAGATGGAGATGGAGCTTTGAATGAAGCTGAATTTTGCATTCTTATGATCAGACTTAGCCCAACTTTGTTTCACCAATCTCATTTGGATAAAAACTTTGGGAGGAAATAGACGTATTCATATTGTTTAATTTTATTTTACTCTTTTTTTTTCTCTCCCTTTTGTTACATTGTATATATTATATTAGCTAAATAATGTAAACTTTGTTTGAATATTTGAGCATTATATAATCTATCTGAGTTGTACTTGTTTTTATATTAAATGTTTGATCATATTTTTGTAATAGTGTGTATTACACGTTTTATGTCTCTAGGCATGGAGAGGGCA

Coding sequence (CDS)

ATGGGATCAGGAAACGGTGTCGTTTTCTTCAAAGATTTCTTCCCTGAAATGGTGGAGAAACTGGGAATTAAAGGGTTCATGAAGGAATTGAGATCTGGGTTTTATTTGATGATGGATAAAGAAAAAGGTCTGATAACTCCAGAAAGCTTGAGAAAAAACATGGGATTTTTGGGATTAATATTGGAAGATGAAGAAGTGATGTTGATGGTGAGAGAAGGCGATTTAGATGGAGATGGAGCTTTGAATGAAGCTGAATTTTGCATTCTTATGATCAGACTTAGCCCAACTTTGTTTCACCAATCTCATTTGGATAAAAACTTTGGGAGGAAATAG

Protein sequence

MGSGNGVVFFKDFFPEMVEKLGIKGFMKELRSGFYLMMDKEKGLITPESLRKNMGFLGLILEDEEVMLMVREGDLDGDGALNEAEFCILMIRLSPTLFHQSHLDKNFGRK
Homology
BLAST of Bhi08G001542 vs. TAIR 10
Match: AT5G54490.1 (pinoid-binding protein 1 )

HSP 1 Score: 102.8 bits (255), Expect = 1.8e-22
Identity = 53/94 (56.38%), Postives = 67/94 (71.28%), Query Frame = 0

Query: 10  FKDFFPEMVEKLGIKGFMKELRSGFYLMMDKEKGLITPESLRKNMG-FLGL-ILEDEEVM 69
           F+DFFP M  KLG +G ++E+  GF L+MDK+KG+IT ESLR+N    LGL  L D++V 
Sbjct: 19  FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLGLGDLTDDDVR 78

Query: 70  LMVREGDLDGDGALNEAEFCILMIRLSPTLFHQS 102
            M+ EGD D DGALN+ EFC+LM RLSP L   S
Sbjct: 79  YMINEGDFDRDGALNQMEFCVLMFRLSPELMEAS 112

BLAST of Bhi08G001542 vs. TAIR 10
Match: AT2G46600.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 102.1 bits (253), Expect = 3.0e-22
Identity = 48/93 (51.61%), Postives = 65/93 (69.89%), Query Frame = 0

Query: 10  FKDFFPEMVEKLGIKGFMKELRSGFYLMMDKEKGLITPESLRKNMGFLGLI-LEDEEVML 69
           ++D  P M EK+ ++ F+ EL  GF L+ D E+ LIT ESLR+N G LG+  +  E+   
Sbjct: 22  YEDMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEGMSKEDAQG 81

Query: 70  MVREGDLDGDGALNEAEFCILMIRLSPTLFHQS 102
           MVREGDLDGDGALN+ EFC+LM+RLSP +   +
Sbjct: 82  MVREGDLDGDGALNQTEFCVLMVRLSPEMMEDA 114

BLAST of Bhi08G001542 vs. TAIR 10
Match: AT4G27280.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 99.4 bits (246), Expect = 1.9e-21
Identity = 53/94 (56.38%), Postives = 65/94 (69.15%), Query Frame = 0

Query: 10  FKDFFPEMVEKLGIKGFMKELRSGFYLMMDKEKGLITPESLRKN-MGFLGL-ILEDEEVM 69
           F DF P M   LG +G + EL +GF L+MD+EKG+IT ESLR+N    LGL  L DE+V 
Sbjct: 19  FHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLGDLTDEDVR 78

Query: 70  LMVREGDLDGDGALNEAEFCILMIRLSPTLFHQS 102
            M++EGD D DGALN+ EFC+LM RLSP L   S
Sbjct: 79  CMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEAS 112

BLAST of Bhi08G001542 vs. TAIR 10
Match: AT1G66410.1 (calmodulin 4 )

HSP 1 Score: 62.0 bits (149), Expect = 3.4e-10
Identity = 34/88 (38.64%), Postives = 48/88 (54.55%), Query Frame = 0

Query: 4   GNGVVFFKDFFPEMVEKLGIKGFMKELRSGFYLMMDKEKGLITPESLRKNMGFLGLILED 63
           GNG + F +F   M +K+      +EL+  F +    + G I+   LR  M  LG  L D
Sbjct: 60  GNGTIDFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTD 119

Query: 64  EEVMLMVREGDLDGDGALNEAEFCILMI 92
           EEV  M+RE D+DGDG +N  EF  +M+
Sbjct: 120 EEVEEMIREADVDGDGQINYEEFVKIMM 147

BLAST of Bhi08G001542 vs. TAIR 10
Match: AT1G66410.2 (calmodulin 4 )

HSP 1 Score: 62.0 bits (149), Expect = 3.4e-10
Identity = 34/88 (38.64%), Postives = 48/88 (54.55%), Query Frame = 0

Query: 4   GNGVVFFKDFFPEMVEKLGIKGFMKELRSGFYLMMDKEKGLITPESLRKNMGFLGLILED 63
           GNG + F +F   M +K+      +EL+  F +    + G I+   LR  M  LG  L D
Sbjct: 70  GNGTIDFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTD 129

Query: 64  EEVMLMVREGDLDGDGALNEAEFCILMI 92
           EEV  M+RE D+DGDG +N  EF  +M+
Sbjct: 130 EEVEEMIREADVDGDGQINYEEFVKIMM 157

BLAST of Bhi08G001542 vs. ExPASy Swiss-Prot
Match: Q9LSQ6 (Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 2.5e-21
Identity = 53/94 (56.38%), Postives = 67/94 (71.28%), Query Frame = 0

Query: 10  FKDFFPEMVEKLGIKGFMKELRSGFYLMMDKEKGLITPESLRKNMG-FLGL-ILEDEEVM 69
           F+DFFP M  KLG +G ++E+  GF L+MDK+KG+IT ESLR+N    LGL  L D++V 
Sbjct: 19  FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLGLGDLTDDDVR 78

Query: 70  LMVREGDLDGDGALNEAEFCILMIRLSPTLFHQS 102
            M+ EGD D DGALN+ EFC+LM RLSP L   S
Sbjct: 79  YMINEGDFDRDGALNQMEFCVLMFRLSPELMEAS 112

BLAST of Bhi08G001542 vs. ExPASy Swiss-Prot
Match: Q9ZPX9 (Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2)

HSP 1 Score: 102.1 bits (253), Expect = 4.2e-21
Identity = 48/93 (51.61%), Postives = 65/93 (69.89%), Query Frame = 0

Query: 10  FKDFFPEMVEKLGIKGFMKELRSGFYLMMDKEKGLITPESLRKNMGFLGLI-LEDEEVML 69
           ++D  P M EK+ ++ F+ EL  GF L+ D E+ LIT ESLR+N G LG+  +  E+   
Sbjct: 22  YEDMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEGMSKEDAQG 81

Query: 70  MVREGDLDGDGALNEAEFCILMIRLSPTLFHQS 102
           MVREGDLDGDGALN+ EFC+LM+RLSP +   +
Sbjct: 82  MVREGDLDGDGALNQTEFCVLMVRLSPEMMEDA 114

BLAST of Bhi08G001542 vs. ExPASy Swiss-Prot
Match: O81831 (Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 2.7e-20
Identity = 53/94 (56.38%), Postives = 65/94 (69.15%), Query Frame = 0

Query: 10  FKDFFPEMVEKLGIKGFMKELRSGFYLMMDKEKGLITPESLRKN-MGFLGL-ILEDEEVM 69
           F DF P M   LG +G + EL +GF L+MD+EKG+IT ESLR+N    LGL  L DE+V 
Sbjct: 19  FHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLGDLTDEDVR 78

Query: 70  LMVREGDLDGDGALNEAEFCILMIRLSPTLFHQS 102
            M++EGD D DGALN+ EFC+LM RLSP L   S
Sbjct: 79  CMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEAS 112

BLAST of Bhi08G001542 vs. ExPASy Swiss-Prot
Match: Q84MN0 (Calmodulin-like protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=CML4 PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 4.4e-10
Identity = 34/87 (39.08%), Postives = 50/87 (57.47%), Query Frame = 0

Query: 4   GNGVVFFKDFFPEMVEKLGIKGFMKELRSGFYLMMDKEKGLITPESLRKNMGFLGLILED 63
           GNG++ F++F   +  K+      +EL+  F ++   + G I+P  LR  M  LG  + D
Sbjct: 59  GNGIIDFQEFLSLIARKMKDGDGDEELKEAFEVLDKDQNGFISPTELRTVMTNLGEKMTD 118

Query: 64  EEVMLMVREGDLDGDGALNEAEFCILM 91
           EEV  M+RE D DGDG +N  EF I+M
Sbjct: 119 EEVEQMIREADTDGDGQVNYDEFVIMM 145

BLAST of Bhi08G001542 vs. ExPASy Swiss-Prot
Match: P43645 (Caltractin (Fragment) OS=Spermatozopsis similis OX=3192 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 5.7e-10
Identity = 34/91 (37.36%), Postives = 52/91 (57.14%), Query Frame = 0

Query: 4   GNGVVFFKDFFPEMVEKLGIKGFMKELRSGFYLMMDKEKGLITPESLRKNMGFLGLILED 63
           G+G + F++F   M  K+G +   +E+   F L  D + G IT ++L++    LG  L D
Sbjct: 56  GSGTIDFEEFLQMMTAKMGERDSREEIMKAFRLFDDDQTGKITFKNLKRVAKELGENLTD 115

Query: 64  EEVMLMVREGDLDGDGALNEAEFCILMIRLS 95
           EE+  M+ E D DGDG +NE EF  +M + S
Sbjct: 116 EEIQEMIDEADRDGDGEINEEEFFRIMKKTS 146

BLAST of Bhi08G001542 vs. ExPASy TrEMBL
Match: A0A5A7UNY8 (Calcium-binding protein PBP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00220 PE=4 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 3.6e-39
Identity = 84/112 (75.00%), Postives = 95/112 (84.82%), Query Frame = 0

Query: 3   SGNGVVFFKDFFPEMVEKLGIKGFMKELRSGFYLMMDKEKGLITPESLRKNMGFLGLILE 62
           SGNGVV FKDFFP+M+EKLG+KGF+KELR GF L+MDKEKG+IT ESLRKNM F GL+LE
Sbjct: 8   SGNGVVIFKDFFPDMLEKLGVKGFIKELRCGFGLLMDKEKGVITAESLRKNMRFFGLVLE 67

Query: 63  DEEVMLMVREGDLDGDGALNEAEFCILMIRLSP-------TLFHQSHLDKNF 108
           DEEVM MVREGDLDGDGAL+EAEF ILMI+LSP       + FHQSHLD+ F
Sbjct: 68  DEEVMWMVREGDLDGDGALSEAEFFILMIKLSPGFMHLSSSFFHQSHLDRKF 119

BLAST of Bhi08G001542 vs. ExPASy TrEMBL
Match: A0A0A0LV00 (EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G071800 PE=4 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.7e-36
Identity = 81/112 (72.32%), Postives = 91/112 (81.25%), Query Frame = 0

Query: 3   SGNGVVFFKDFFPEMVEKLGIKGFMKELRSGFYLMMDKEKGLITPESLRKNMGFLGLILE 62
           SG  VV FKDFFPEM+EKLG KGF+KELR GF L+MDKEKG+IT ESLRKNM F GL+LE
Sbjct: 6   SGKDVVLFKDFFPEMLEKLGAKGFIKELRYGFRLLMDKEKGVITAESLRKNMRFFGLVLE 65

Query: 63  DEEVMLMVREGDLDGDGALNEAEFCILMIRLSP-------TLFHQSHLDKNF 108
           DEEVM MV+EGDLDGDGAL+EAEF ILMI+LSP       + FHQSH D+ F
Sbjct: 66  DEEVMWMVKEGDLDGDGALSEAEFFILMIKLSPGFMHLSSSFFHQSHSDRKF 117

BLAST of Bhi08G001542 vs. ExPASy TrEMBL
Match: A0A7N2LV84 (EF-hand domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 3.7e-28
Identity = 69/102 (67.65%), Postives = 81/102 (79.41%), Query Frame = 0

Query: 1   MGSGNGVVFFKDFFPEMVEKLGIKGFMKELRSGFYLMMDKEKGLITPESLRKNMGFLGLI 60
           MGS NGVV F+DFFP MVEKLG +GFMKEL +GF L+MD+EKG+IT +SL++N   LGL 
Sbjct: 1   MGSRNGVV-FEDFFPAMVEKLGAEGFMKELDNGFRLLMDEEKGVITFQSLKRNSALLGLQ 60

Query: 61  -LEDEEVMLMVREGDLDGDGALNEAEFCILMIRLSPTLFHQS 102
            + DEE+M M+REGDLDGDGALNE EFC LM RLSP L   S
Sbjct: 61  GMSDEELMCMLREGDLDGDGALNEMEFCTLMFRLSPGLMESS 101

BLAST of Bhi08G001542 vs. ExPASy TrEMBL
Match: A0A067GJU9 (EF-hand domain-containing protein OS=Citrus sinensis OX=2711 GN=CISIN_1g033591mg PE=4 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 1.1e-27
Identity = 67/102 (65.69%), Postives = 78/102 (76.47%), Query Frame = 0

Query: 1   MGSGNGVVFFKDFFPEMVEKLGIKGFMKELRSGFYLMMDKEKGLITPESLRKNMGFLGL- 60
           M S N  V F+DFFP MVEKLG +GFM+EL +GF L+MD++KGLIT ESL+KN   LGL 
Sbjct: 1   MASANSGVVFEDFFPAMVEKLGAEGFMQELSNGFRLLMDEDKGLITFESLKKNSALLGLQ 60

Query: 61  ILEDEEVMLMVREGDLDGDGALNEAEFCILMIRLSPTLFHQS 102
            + DEE+M M+REGDLDGDGALNE EFC LM RLSP L   S
Sbjct: 61  DMSDEELMCMLREGDLDGDGALNEMEFCTLMFRLSPGLMKNS 102

BLAST of Bhi08G001542 vs. ExPASy TrEMBL
Match: A0A2P5BKI3 (Parvalbumin OS=Parasponia andersonii OX=3476 GN=PanWU01x14_230790 PE=4 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.8e-27
Identity = 66/100 (66.00%), Postives = 78/100 (78.00%), Query Frame = 0

Query: 3   SGNGVVFFKDFFPEMVEKLGIKGFMKELRSGFYLMMDKEKGLITPESLRKNMGFLGLI-L 62
           S NG V F+DFFP MV+KLG +GFMKEL +GF+L+MDKEKG+IT ESL++N   LGL  +
Sbjct: 4   SRNGGVVFEDFFPAMVDKLGQEGFMKELCNGFHLLMDKEKGVITFESLKENAALLGLQGI 63

Query: 63  EDEEVMLMVREGDLDGDGALNEAEFCILMIRLSPTLFHQS 102
            DEE+M MVREGD+DGD ALNE EFC LM RLSP L   S
Sbjct: 64  SDEEIMCMVREGDMDGDQALNEMEFCTLMFRLSPALMENS 103

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G54490.11.8e-2256.38pinoid-binding protein 1 [more]
AT2G46600.13.0e-2251.61Calcium-binding EF-hand family protein [more]
AT4G27280.11.9e-2156.38Calcium-binding EF-hand family protein [more]
AT1G66410.13.4e-1038.64calmodulin 4 [more]
AT1G66410.23.4e-1038.64calmodulin 4 [more]
Match NameE-valueIdentityDescription
Q9LSQ62.5e-2156.38Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1[more]
Q9ZPX94.2e-2151.61Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2[more]
O818312.7e-2056.38Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1[more]
Q84MN04.4e-1039.08Calmodulin-like protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=CML4 PE=2 ... [more]
P436455.7e-1037.36Caltractin (Fragment) OS=Spermatozopsis similis OX=3192 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7UNY83.6e-3975.00Calcium-binding protein PBP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A0A0LV001.7e-3672.32EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G071800 PE=... [more]
A0A7N2LV843.7e-2867.65EF-hand domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A067GJU91.1e-2765.69EF-hand domain-containing protein OS=Citrus sinensis OX=2711 GN=CISIN_1g033591mg... [more]
A0A2P5BKI31.8e-2766.00Parvalbumin OS=Parasponia andersonii OX=3476 GN=PanWU01x14_230790 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 41..90
e-value: 4.9E-14
score: 51.8
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 61..96
score: 9.63171
NoneNo IPR availableGENE3D1.10.238.10coord: 8..108
e-value: 2.4E-15
score: 58.4
NoneNo IPR availablePANTHERPTHR47319:SF5CALCIUM-BINDING PROTEIN PBP1-LIKEcoord: 1..102
IPR044205Calcium-binding protein KIC/PBP1/KRP1PANTHERPTHR47319CALCIUM-BINDING PROTEIN KICcoord: 1..102
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 74..86
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 3..90

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M001542Bhi08M001542mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding