Bhi08G001427 (gene) Wax gourd (B227) v1

Overview


Name	Bhi08G001427
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	protein HEAT-STRESS-ASSOCIATED 32
Location	chr8: 50399798 .. 50402094 (+)
RNA-Seq Expression	Bhi08G001427
Synteny	Bhi08G001427

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAGTTTCCGAAAGAAACAGATGCAAAAAGTGGAGGAACTATTTGTAAATATGTCTAAATTTTGCGTAGGGAGGAGTAGGGAGAAGTTATCTGTTTCCGATTCACCCTCCATTGATCACTGCCTTCGAGAAAATGTCAGCTTATTTGTGGAAAAGCTTCGACGAGGATGAAGATCGACCGGAGAAGCCTCGGCGATGTGGAGTTACCGAGATGAGAGGCCCTCATTACACTCTCTCAAGCCAAAATGTTATTCAGGTTCGCGTGTTCTTCTTCTATATTTCGGGCGAGCGAAGAATTTTGTATCCATGTGTCCATTTCTTTGTGTTACGGTGTTAGATGATTTCCGCTTATTGATCGCTTGTTTCTCTTTTTAATATCATTCCCCAAATTGGTACTGTGAAGGAAATTTTTGAGTCTGTGGGTCAATTTGTCGATGGGTTAAAATTCTCCGGGGGTTCTCACAGCCTGTTGCCGAGGGATTTTATGAAAGAGGTGACGGAAATGGCTCATCGACATGACATCTATGTCAGTACGGGTGACTGGGCGGAGCATTTACTTCGCAAAGGTCCATCGGGTTTCAAAGAGTATGTGGAGGTGAGAAGAAGAGGAATGCCAATAATTTTGTTGTTTTTAATTCTGTGACAGAAGCTGGTGTGATAATTTGGTGTATGGTGTTTCTAGGAATGTAAGCTCTTGGGATTTGACACTATTGAGCTCAACGTGGGATCACTTGAAGTTCCTGAAGAAACTCTTCTGAGATACGTGCGCTTGATTAAGAACGGTGGCCTTAGGGCTAAGCCTCAGTTTGCAGTTAAGTTCAACAAGTCTGATATTCCCGTCAGTGGCAATCGCGCATTTGGAGCTTATATTGTTCCAAGACCACGATCTTCTGGTAAGGTTGCCATTTGCAAATCTGTTCTTCTCAAGAAATGGGTCTAGGATTTGCTTTGGGTTTTGAAAAACCAATCAGATAAACTGTTGACTGTTCTAAGTTTGTCATTTGTAAGATGATCACTCGAGTGCAGAACATAGTTATTAAAATAAATACATTGAATGCAACGATTTCTTTATATTCACAGATTCAATCATCAAAATTTCGCTGACAAAAAAGGGTATAATCATTTGATGGCATGAAACAGCGATCAGCCATCTTTGTATTTCCATGGCTTGCTCTGTTATGCAATCTGCATTTCTAGTTCTCTATTTGGTGAGTTTGATACTGAATACTTGGAAAGAAAAACTTCCAATCTTTTGTTATGGTTTAATCAAGATGGCCTTTGATCCTACTGCATAACTACTGATATGGTGATTCTATCTAGCCTTGCAATTTGTCATAGATGGAATTGATTTTCATAATTATTGCTCTAGTTGTATTTTATTGCATGCTTGATCTTATGAAATCAAGTCAATTTTATAATCTTAAATTGATATTGAATTTGAAGAATATAGAATCAATGGCTTTATTTTGGGCATTGAAATCGAAAAACAAGTTGAACATGGCACTACAGGTGCAATGTAGTTCACTTGCATCATGCCCATAGCTCATTCTTTATTAGTCTTGATAAGCTCTCTAAACCACAAAGCCTTTTCAATTAGTTTAGCTTTCGTTGACAAAAAACAGTCTCTATGGCCATTGTTTGTAAACCTTGGGCTTTTGTCACTGTAACTTGCAGAACTTGTAGAAGATGTTGATCTTTTGATCAGAAGGGCTGAGAGATGCCTAGAAGCTGGGGCAGACATGATAATGATCGATGCAGACGATGTCAGTAAACATGCCGAGTCTCTTCGAACAGACATTATTGCCAAGATCATTGGGCGTCTTGGTCTAGAGAAAACGATGTTTGAAGCATCGAATCCCCAAACATCAGAGTGGTTTATCAATCATTACGGGCCAAAGGTATGAAAAGATTATCTTCCTATGGTTATATTTTCAATCCTTTACTCAATGACATTCTTGTATGTCCTGTATTTAGGTGAATCTTTTTGTGGATCACTCTCAAGTTATGGATCTGGAGTGCCTTCGGGGCCGCAACCTGGGTAAAAATCATTCATCTATTCTTGGTTCGTCCTACCTTCGGATTTGAAGTCTGTGTCAACTCCAGAGATTTATGTTGGGTTCTATATATGAACTTCAACATTGCATTGTTGCAGTGTGTTTTGATGCTTTTAGATAAATTGCAATACACCCTTTTTTTCTCTTCTTGATCAGAACTCCAAAATGTATTTCTTTACTTTTCTTTAGTAAATGAAATGGTTCCTGGATGAATGAAATAACATATTTTGACTGTTGTATCAA

mRNA sequence

CAAAGTTTCCGAAAGAAACAGATGCAAAAAGTGGAGGAACTATTTGTAAATATGTCTAAATTTTGCGTAGGGAGGAGTAGGGAGAAGTTATCTGTTTCCGATTCACCCTCCATTGATCACTGCCTTCGAGAAAATGTCAGCTTATTTGTGGAAAAGCTTCGACGAGGATGAAGATCGACCGGAGAAGCCTCGGCGATGTGGAGTTACCGAGATGAGAGGCCCTCATTACACTCTCTCAAGCCAAAATGTTATTCAGGAAATTTTTGAGTCTGTGGGTCAATTTGTCGATGGGTTAAAATTCTCCGGGGGTTCTCACAGCCTGTTGCCGAGGGATTTTATGAAAGAGGTGACGGAAATGGCTCATCGACATGACATCTATGTCAGTACGGGTGACTGGGCGGAGCATTTACTTCGCAAAGGTCCATCGGGTTTCAAAGAGTATGTGGAGGAATGTAAGCTCTTGGGATTTGACACTATTGAGCTCAACGTGGGATCACTTGAAGTTCCTGAAGAAACTCTTCTGAGATACGTGCGCTTGATTAAGAACGGTGGCCTTAGGGCTAAGCCTCAGTTTGCAGTTAAGTTCAACAAGTCTGATATTCCCGTCAGTGGCAATCGCGCATTTGGAGCTTATATTGTTCCAAGACCACGATCTTCTGAACTTGTAGAAGATGTTGATCTTTTGATCAGAAGGGCTGAGAGATGCCTAGAAGCTGGGGCAGACATGATAATGATCGATGCAGACGATGTCAGTAAACATGCCGAGTCTCTTCGAACAGACATTATTGCCAAGATCATTGGGCGTCTTGGTCTAGAGAAAACGATGTTTGAAGCATCGAATCCCCAAACATCAGAGTGGTTTATCAATCATTACGGGCCAAAGGTGAATCTTTTTGTGGATCACTCTCAAGTTATGGATCTGGAGTGCCTTCGGGGCCGCAACCTGGGTAAAAATCATTCATCTATTCTTGGTTCGTCCTACCTTCGGATTTGAAGTCTGTGTCAACTCCAGAGATTTATGTTGGGTTCTATATATGAACTTCAACATTGCATTGTTGCAGTGTGTTTTGATGCTTTTAGATAAATTGCAATACACCCTTTTTTTCTCTTCTTGATCAGAACTCCAAAATGTATTTCTTTACTTTTCTTTAGTAAATGAAATGGTTCCTGGATGAATGAAATAACATATTTTGACTGTTGTATCAA

Coding sequence (CDS)

ATGTCAGCTTATTTGTGGAAAAGCTTCGACGAGGATGAAGATCGACCGGAGAAGCCTCGGCGATGTGGAGTTACCGAGATGAGAGGCCCTCATTACACTCTCTCAAGCCAAAATGTTATTCAGGAAATTTTTGAGTCTGTGGGTCAATTTGTCGATGGGTTAAAATTCTCCGGGGGTTCTCACAGCCTGTTGCCGAGGGATTTTATGAAAGAGGTGACGGAAATGGCTCATCGACATGACATCTATGTCAGTACGGGTGACTGGGCGGAGCATTTACTTCGCAAAGGTCCATCGGGTTTCAAAGAGTATGTGGAGGAATGTAAGCTCTTGGGATTTGACACTATTGAGCTCAACGTGGGATCACTTGAAGTTCCTGAAGAAACTCTTCTGAGATACGTGCGCTTGATTAAGAACGGTGGCCTTAGGGCTAAGCCTCAGTTTGCAGTTAAGTTCAACAAGTCTGATATTCCCGTCAGTGGCAATCGCGCATTTGGAGCTTATATTGTTCCAAGACCACGATCTTCTGAACTTGTAGAAGATGTTGATCTTTTGATCAGAAGGGCTGAGAGATGCCTAGAAGCTGGGGCAGACATGATAATGATCGATGCAGACGATGTCAGTAAACATGCCGAGTCTCTTCGAACAGACATTATTGCCAAGATCATTGGGCGTCTTGGTCTAGAGAAAACGATGTTTGAAGCATCGAATCCCCAAACATCAGAGTGGTTTATCAATCATTACGGGCCAAAGGTGAATCTTTTTGTGGATCACTCTCAAGTTATGGATCTGGAGTGCCTTCGGGGCCGCAACCTGGGTAAAAATCATTCATCTATTCTTGGTTCGTCCTACCTTCGGATTTGA

Protein sequence

MSAYLWKSFDEDEDRPEKPRRCGVTEMRGPHYTLSSQNVIQEIFESVGQFVDGLKFSGGSHSLLPRDFMKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVGSLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPVSGNRAFGAYIVPRPRSSELVEDVDLLIRRAERCLEAGADMIMIDADDVSKHAESLRTDIIAKIIGRLGLEKTMFEASNPQTSEWFINHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSYLRI

Homology

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BLAST of Bhi08G001427 vs. TAIR 10
Match: AT4G21320.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 469.5 bits (1207), Expect = 1.9e-132
Identity = 219/280 (78.21%), Postives = 249/280 (88.93%), Query Frame = 0


Query: 4   YLWKSFDEDEDRPEKPRRCGVTEMRGPHYTLSSQNVIQEIFESVGQFVDGLKFSGGSHSL 63
           Y WKSF+E+EDRPEKPRR GVTEMRGPHY++ SQN++QEIFES+GQFVDGLKFSGGS+SL
Sbjct: 5   YRWKSFEENEDRPEKPRRYGVTEMRGPHYSVLSQNLLQEIFESMGQFVDGLKFSGGSNSL 64

Query: 64  LPRDFMKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVGSLE 123
           +P+ F+K+  EMAH H +YVSTGDWAEH+LR GPS FK+YVEECK LGFDTIELN   LE
Sbjct: 65  IPKSFIKQAIEMAHEHGVYVSTGDWAEHMLRSGPSAFKDYVEECKQLGFDTIELNANLLE 124

Query: 124 VPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPVSGNRAFGAYIVPRPRSSELVEDVDL 183
           VPEETLLRYVRLIKNGGLRAKP FAVKFNKSDIP   NRAFG+Y+VP PRSSE VED+DL
Sbjct: 125 VPEETLLRYVRLIKNGGLRAKPMFAVKFNKSDIP-GRNRAFGSYVVPEPRSSEFVEDIDL 184

Query: 184 LIRRAERCLEAGADMIMIDADDVSKHAESLRTDIIAKIIGRLGLEKTMFEASNPQTSEWF 243
           LIR+AERCLEAGAD IMIDADDV K+A+SLR DIIAK+IGRLG+EKTMFEAS+ +  EWF
Sbjct: 185 LIRKAERCLEAGADTIMIDADDVCKYADSLRADIIAKVIGRLGIEKTMFEASDAKLVEWF 244

Query: 244 INHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSY 284
           I  YGP VNL+VDHSQ+MDLECLRGR+LGK+H S+L SSY
Sbjct: 245 IKRYGPNVNLYVDHSQIMDLECLRGRHLGKDHQSVLSSSY 283

[Close]

BLAST of Bhi08G001427 vs. ExPASy Swiss-Prot
Match: Q8GWL1 (Protein HEAT-STRESS-ASSOCIATED 32 OS=Arabidopsis thaliana OX=3702 GN=HSA32 PE=2 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 2.6e-131
Identity = 219/280 (78.21%), Postives = 249/280 (88.93%), Query Frame = 0


Query: 4   YLWKSFDEDEDRPEKPRRCGVTEMRGPHYTLSSQNVIQEIFESVGQFVDGLKFSGGSHSL 63
           Y WKSF+E+EDRPEKPRR GVTEMRGPHY++ SQN++QEIFES+GQFVDGLKFSGGS+SL
Sbjct: 5   YRWKSFEENEDRPEKPRRYGVTEMRGPHYSVLSQNLLQEIFESMGQFVDGLKFSGGSNSL 64

Query: 64  LPRDFMKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVGSLE 123
           +P+ F+K+  EMAH H +YVSTGDWAEH+LR GPS FK+YVEECK LGFDTIELN   LE
Sbjct: 65  IPKSFIKQAIEMAHEHGVYVSTGDWAEHMLRSGPSAFKDYVEECKQLGFDTIELNANLLE 124

Query: 124 VPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPVSGNRAFGAYIVPRPRSSELVEDVDL 183
           VPEETLLRYVRLIKNGGLRAKP FAVKFNKSDIP   NRAFG+Y+VP PRSSE VED+DL
Sbjct: 125 VPEETLLRYVRLIKNGGLRAKPMFAVKFNKSDIP-GRNRAFGSYVVPEPRSSEFVEDIDL 184

Query: 184 LIRRAERCLEAGADMIMIDADDVSKHAESLRTDIIAKIIGRLGLEKTMFEASNPQTSEWF 243
           LIR+AERCLEAGAD IMIDADDV K+A+SLR DIIAK+IGRLG+EKTMFEAS+ +  EWF
Sbjct: 185 LIRKAERCLEAGADTIMIDADDVCKYADSLRADIIAKVIGRLGIEKTMFEASDAKLVEWF 244

Query: 244 INHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSY 284
           I  YGP VNL+VDHSQ+MDLECLRGR+LGK+H S+L SSY
Sbjct: 245 IKRYGPNVNLYVDHSQIMDLECLRGRHLGKDHQSVLSSSY 283

[Close]

BLAST of Bhi08G001427 vs. ExPASy Swiss-Prot
Match: O27710 (Phosphosulfolactate synthase OS=Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) OX=187420 GN=comA PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.5e-14
Identity = 67/270 (24.81%), Postives = 115/270 (42.59%), Query Frame = 0


Query: 15  RPEKPRRCGVTEMRGPHYTLSSQNVIQEIFESVGQFVDGLKFSGGSHSLLPRDFMKEVTE 74
           R  KPR+ G+T +       +S    +++ E    +VD +KF  G+  L  RD +KE  +
Sbjct: 11  RSGKPRKNGITMVLDKGMGPAS---ARDLMEISSDYVDFIKFGWGTLPLHRRDTVKEKVD 70

Query: 75  MAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVGSLEVPEETLLRYVR 134
           M    D+    G     +        +EY +E + LGF+T+E++ G++E+  E   R + 
Sbjct: 71  MYRSFDVEPYPGGTLFEIAHLNDK-VEEYFQEARSLGFETLEISNGTVEIETEEKCRLIE 130

Query: 135 LIKNGGLRAKPQFAVKFNKSDIPVSGNRAFGAYIVPRPRSSELVEDVDLLIRRAERCLEA 194
           +  + G     +   K                     P    L+E  D  +R     L A
Sbjct: 131 MAVDEGFMVLSEVGKK--------------------DPERDRLLEPDD-RVRLVRADLRA 190

Query: 195 GADMIMIDADD------VSKHAESLRTDIIAKIIGRLGLEKTMFEASNPQTSEWFINHYG 254
           GA M++++A +      +     ++R D    +  RL +++ ++EA       +FI   G
Sbjct: 191 GASMVLMEARESGQNIGIYDERGNIREDEFNHLTDRLPMDRIIWEAPQKSQQVYFILKIG 250

Query: 255 PKVNL-FVDHSQVMDLEC----LRGRNLGK 274
           P VNL  +   ++  LE     LRG  LGK
Sbjct: 251 PDVNLGNIPPEEITALETIRRGLRGDTLGK 255

[Close]

BLAST of Bhi08G001427 vs. ExPASy Swiss-Prot
Match: Q57703 (Phosphosulfolactate synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=comA PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 9.3e-12
Identity = 57/239 (23.85%), Postives = 102/239 (42.68%), Query Frame = 0


Query: 40  IQEIFESVGQFVDGLKFSGGSHSLLPRDFMKEVTEMAHRHDIYVSTGDWA-EHLLRKGPS 99
           +++  +  G ++D +KF  G+ +++ RD +KE         I V  G    E+   KG  
Sbjct: 28  VEDYLKVCGDYIDFVKFGWGTSAVIDRDVVKEKINYYKDWGIKVYPGGTLFEYAYSKGK- 87

Query: 100 GFKEYVEECKLLGFDTIELNVGSLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPV 159
            F E++ EC+ LGF+ +E++ GS ++  E     ++  K+ G     +   K    D  +
Sbjct: 88  -FDEFLNECEKLGFEAVEISDGSSDISLEERKNAIKRAKDNGFMVLTEVGKKMPDKDKQL 147

Query: 160 SGNRAFGAYIVPRPRSSELVEDVDLLIRRAERCLEAGADMIMIDADDVSK---------H 219
           +                     +D  I+     L+AGAD ++I+  +  K          
Sbjct: 148 T---------------------IDDRIKLINFDLDAGADYVIIEGRESGKGIGLFDKEGK 207

Query: 220 AESLRTDIIAKIIGRLGLEKTMFEASNPQTSEWFINHYGPKVNLF-VDHSQVMDLECLR 268
            +    D++AK    + + K +FEA        FI  +G  VNL  +   +V+ LE LR
Sbjct: 208 VKENELDVLAK---NVDINKVIFEAPQKSQQVAFILKFGSSVNLANIAFDEVISLETLR 240

[Close]

BLAST of Bhi08G001427 vs. ExPASy TrEMBL
Match: A0A0A0LUX3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G066510 PE=3 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 5.6e-161
Identity = 276/286 (96.50%), Postives = 283/286 (98.95%), Query Frame = 0


Query: 1   MSAYLWKSFDEDEDRPEKPRRCGVTEMRGPHYTLSSQNVIQEIFESVGQFVDGLKFSGGS 60
           MSAYLWKSFDEDEDRPEKPRRCGVTEMRGPHYTLS+Q+VIQEIFES+GQFVDGLKFSGGS
Sbjct: 1   MSAYLWKSFDEDEDRPEKPRRCGVTEMRGPHYTLSTQDVIQEIFESLGQFVDGLKFSGGS 60

Query: 61  HSLLPRDFMKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVG 120
           HSLLPRDFMKEVTEMAHRHD+YVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVG
Sbjct: 61  HSLLPRDFMKEVTEMAHRHDVYVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVG 120

Query: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPVSGNRAFGAYIVPRPRSSELVED 180
           SL+VPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIP+SGNRAFGAYIVP+PRSSELVED
Sbjct: 121 SLDVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPISGNRAFGAYIVPKPRSSELVED 180

Query: 181 VDLLIRRAERCLEAGADMIMIDADDVSKHAESLRTDIIAKIIGRLGLEKTMFEASNPQTS 240
           VDLLIRRAERCLEAGADMIMIDADDVSKHAE LR DIIAKIIGRLGLEKTMFEASNPQTS
Sbjct: 181 VDLLIRRAERCLEAGADMIMIDADDVSKHAEFLRADIIAKIIGRLGLEKTMFEASNPQTS 240

Query: 241 EWFINHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSYLRI 287
           EWFINHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSY RI
Sbjct: 241 EWFINHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSYFRI 286

[Close]

BLAST of Bhi08G001427 vs. ExPASy TrEMBL
Match: A0A5A7UNV6 (Protein HEAT-STRESS-ASSOCIATED 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G006250 PE=3 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 2.8e-160
Identity = 275/286 (96.15%), Postives = 282/286 (98.60%), Query Frame = 0


Query: 1   MSAYLWKSFDEDEDRPEKPRRCGVTEMRGPHYTLSSQNVIQEIFESVGQFVDGLKFSGGS 60
           MSAYLWKSFDEDEDRPEKPRR GVTEMRGPHYTLS+QNVIQEIFESVGQFVDGLKFSGGS
Sbjct: 1   MSAYLWKSFDEDEDRPEKPRRSGVTEMRGPHYTLSTQNVIQEIFESVGQFVDGLKFSGGS 60

Query: 61  HSLLPRDFMKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVG 120
           HSLLPRDFMKEVTEMAHRHD+YVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVG
Sbjct: 61  HSLLPRDFMKEVTEMAHRHDVYVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVG 120

Query: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPVSGNRAFGAYIVPRPRSSELVED 180
           SL+VPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIP++GNRAFGAYIVP+PRSSE VED
Sbjct: 121 SLDVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPITGNRAFGAYIVPKPRSSEFVED 180

Query: 181 VDLLIRRAERCLEAGADMIMIDADDVSKHAESLRTDIIAKIIGRLGLEKTMFEASNPQTS 240
           VDLLIRRAERCLEAGADMIMIDADDVS+HAESLR DIIAKIIGRLGLEKTMFEASNPQTS
Sbjct: 181 VDLLIRRAERCLEAGADMIMIDADDVSRHAESLRADIIAKIIGRLGLEKTMFEASNPQTS 240

Query: 241 EWFINHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSYLRI 287
           EWFINHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSY RI
Sbjct: 241 EWFINHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSYFRI 286

[Close]

BLAST of Bhi08G001427 vs. ExPASy TrEMBL
Match: A0A1S3B4M6 (protein HEAT-STRESS-ASSOCIATED 32 OS=Cucumis melo OX=3656 GN=LOC103485727 PE=3 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 2.8e-160
Identity = 275/286 (96.15%), Postives = 282/286 (98.60%), Query Frame = 0


Query: 1   MSAYLWKSFDEDEDRPEKPRRCGVTEMRGPHYTLSSQNVIQEIFESVGQFVDGLKFSGGS 60
           MSAYLWKSFDEDEDRPEKPRR GVTEMRGPHYTLS+QNVIQEIFESVGQFVDGLKFSGGS
Sbjct: 1   MSAYLWKSFDEDEDRPEKPRRSGVTEMRGPHYTLSTQNVIQEIFESVGQFVDGLKFSGGS 60

Query: 61  HSLLPRDFMKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVG 120
           HSLLPRDFMKEVTEMAHRHD+YVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVG
Sbjct: 61  HSLLPRDFMKEVTEMAHRHDVYVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVG 120

Query: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPVSGNRAFGAYIVPRPRSSELVED 180
           SL+VPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIP++GNRAFGAYIVP+PRSSE VED
Sbjct: 121 SLDVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPITGNRAFGAYIVPKPRSSEFVED 180

Query: 181 VDLLIRRAERCLEAGADMIMIDADDVSKHAESLRTDIIAKIIGRLGLEKTMFEASNPQTS 240
           VDLLIRRAERCLEAGADMIMIDADDVS+HAESLR DIIAKIIGRLGLEKTMFEASNPQTS
Sbjct: 181 VDLLIRRAERCLEAGADMIMIDADDVSRHAESLRADIIAKIIGRLGLEKTMFEASNPQTS 240

Query: 241 EWFINHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSYLRI 287
           EWFINHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSY RI
Sbjct: 241 EWFINHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSYFRI 286

[Close]

BLAST of Bhi08G001427 vs. ExPASy TrEMBL
Match: A0A6J1CDT4 (protein HEAT-STRESS-ASSOCIATED 32 OS=Momordica charantia OX=3673 GN=LOC111010764 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.1e-153
Identity = 264/286 (92.31%), Postives = 277/286 (96.85%), Query Frame = 0


Query: 1   MSAYLWKSFDEDEDRPEKPRRCGVTEMRGPHYTLSSQNVIQEIFESVGQFVDGLKFSGGS 60
           MSAY WKSFDEDEDRPEKPRR GVTEMRGPHYTLSSQNVIQ+IFES+GQFVDG+KFSGGS
Sbjct: 1   MSAYWWKSFDEDEDRPEKPRRFGVTEMRGPHYTLSSQNVIQDIFESMGQFVDGIKFSGGS 60

Query: 61  HSLLPRDFMKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVG 120
           HSLL RDF+K+VTEMAH+HDIYVSTGDWAEHLLRKGPSGF EYVEECKLLGFDT+ELNVG
Sbjct: 61  HSLLSRDFVKKVTEMAHKHDIYVSTGDWAEHLLRKGPSGFNEYVEECKLLGFDTVELNVG 120

Query: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPVSGNRAFGAYIVPRPRSSELVED 180
           SLEVPEET+LRYVRLIKNGGLRAKPQFAVKFNKSDIP+SGN AFGAYIVPRPRSSELVED
Sbjct: 121 SLEVPEETILRYVRLIKNGGLRAKPQFAVKFNKSDIPISGNPAFGAYIVPRPRSSELVED 180

Query: 181 VDLLIRRAERCLEAGADMIMIDADDVSKHAESLRTDIIAKIIGRLGLEKTMFEASNPQTS 240
           VDLLIR+AERCLEAGADMIMIDADDVSKHAE+LR DIIAKIIGRLGLEKTMFEASNP+TS
Sbjct: 181 VDLLIRKAERCLEAGADMIMIDADDVSKHAETLRADIIAKIIGRLGLEKTMFEASNPRTS 240

Query: 241 EWFINHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSYLRI 287
           EWFI  YGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSY +I
Sbjct: 241 EWFIKQYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSYFQI 286

[Close]

BLAST of Bhi08G001427 vs. ExPASy TrEMBL
Match: A0A6J1K2U1 (protein HEAT-STRESS-ASSOCIATED 32 OS=Cucurbita maxima OX=3661 GN=LOC111490151 PE=3 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.1e-148
Identity = 259/283 (91.52%), Postives = 271/283 (95.76%), Query Frame = 0


Query: 1   MSAYLWKSFDEDEDRPEKPRRCGVTEMRGPHYTLSSQNVIQEIFESVGQFVDGLKFSGGS 60
           MSAY WK+F  DEDRPEKPRRCGVTEMR PHYTLSSQNVIQEIFES+GQFVDGLKFSGGS
Sbjct: 38  MSAYWWKTFAGDEDRPEKPRRCGVTEMRSPHYTLSSQNVIQEIFESMGQFVDGLKFSGGS 97

Query: 61  HSLLPRDFMKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLLGFDTIELNVG 120
           HSLLPR+F+KEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKL+GFDTIELNV 
Sbjct: 98  HSLLPREFVKEVTEMAHRHDIYVSTGDWAEHLLRKGPSGFKEYVEECKLMGFDTIELNVD 157

Query: 121 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIPVSGNRAFGAYIVPRPRSSELVED 180
           SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIP +G+RAFGAYIVPRPRSSELVED
Sbjct: 158 SLEVPEETLLRYVRLIKNGGLRAKPQFAVKFNKSDIP-AGDRAFGAYIVPRPRSSELVED 217

Query: 181 VDLLIRRAERCLEAGADMIMIDADDVSKHAESLRTDIIAKIIGRLGLEKTMFEASNPQTS 240
           VDLLIRRAERCLEAGADMIMIDADDVSKH +SLR DIIAKIIGRLGLEKTMFEASNP+T 
Sbjct: 218 VDLLIRRAERCLEAGADMIMIDADDVSKHTDSLRADIIAKIIGRLGLEKTMFEASNPKTL 277

Query: 241 EWFINHYGPKVNLFVDHSQVMDLECLRGRNLGKNHSSILGSSY 284
           EWFIN Y  KVNLFVDHSQVM+LECLRGRNLGKNHSS+LGS+Y
Sbjct: 278 EWFINQYSSKVNLFVDHSQVMELECLRGRNLGKNHSSVLGSAY 319

The following BLAST results are available for this feature:

BLAST of Bhi08G001427 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21320.1	1.9e-132	78.21	Aldolase-type TIM barrel family protein	[more]

BLAST of Bhi08G001427 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GWL1	2.6e-131	78.21	Protein HEAT-STRESS-ASSOCIATED 32 OS=Arabidopsis thaliana OX=3702 GN=HSA32 PE=2 ...	[more]
O27710	4.5e-14	24.81	Phosphosulfolactate synthase OS=Methanothermobacter thermautotrophicus (strain A...	[more]
Q57703	9.3e-12	23.85	Phosphosulfolactate synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067...	[more]

BLAST of Bhi08G001427 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LUX3	5.6e-161	96.50	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G066510 PE=3 SV=1	[more]
A0A5A7UNV6	2.8e-160	96.15	Protein HEAT-STRESS-ASSOCIATED 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3B4M6	2.8e-160	96.15	protein HEAT-STRESS-ASSOCIATED 32 OS=Cucumis melo OX=3656 GN=LOC103485727 PE=3 S...	[more]
A0A6J1CDT4	1.1e-153	92.31	protein HEAT-STRESS-ASSOCIATED 32 OS=Momordica charantia OX=3673 GN=LOC111010764...	[more]
A0A6J1K2U1	1.1e-148	91.52	protein HEAT-STRESS-ASSOCIATED 32 OS=Cucurbita maxima OX=3661 GN=LOC111490151 PE...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003830	(2R)-phospho-3-sulpholactate synthase, ComA	PFAM	PF02679	ComA	coord: 14..271 e-value: 4.9E-81 score: 271.8
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 8..274 e-value: 1.1E-81 score: 275.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR33116	REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED	coord: 13..285
None	No IPR available	PANTHER	PTHR33116:SF50	PROTEIN HEAT-STRESS-ASSOCIATED 32	coord: 13..285
IPR036112	(2R)-phospho-3-sulpholactate synthase, ComA superfamily	SUPERFAMILY	102110	(2r)-phospho-3-sulfolactate synthase ComA	coord: 13..267

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi08M001427	Bhi08M001427	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010286	heat acclimation
biological_process	GO:0010608	posttranscriptional regulation of gene expression
molecular_function	GO:0003824	catalytic activity