Bhi08G001304 (gene) Wax gourd (B227) v1

Overview
NameBhi08G001304
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionBHLH domain-containing protein
Locationchr8: 45825908 .. 45826911 (+)
RNA-Seq ExpressionBhi08G001304
SyntenyBhi08G001304
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAACGTTGGCAATGGTCAAATTTCTTCTTCTTCTTTGAAACTTGATCGCAAAACTATCGAGAAAAATAGAAGAATCCATATGAAATCTCTCTGTTCTAAACTTTTTAATCTTGTTCCTCCCTCCCATTTCAAAATCTCTAAGGTTAATTCTCTCTCTCAACTCCATCCATGCTCTTATACCATATTAGATAATAGAGTTTTCATCTCAAAACCAATTGATAATAAAAAAAAAAAGTAGTTTATTTGAGGACCATTGATTTTACCATGTGGGATCTTCAATAGTCGTCCTTAATATTTAATTTTTTTTTTTTTTCAGTTTGGTTATTTTTACCATCAATAAAACATTATAAAGGTAGCTGATGAAATATATATATATATATTTGAACATTAAGGACTAAAACATCTTCTCATTAATGTTGCATTGCACGTGTGTATTTTTTCTTTTGTTTGTTTCACAGGAATTGTTATCGCAACAAAATCAAATCTCCTATGTAATTGCTTACATAAATGAATTGAAAGAAAGAGTGGAGAATTTGGAGAAAAGGAAACAAGCTTTACAATTAAGAAACCATTCTAATAATCATGTTCAGCAAGCTAATCTCCATAGGGTTTCGACAAGGATGATAGCTGATAATGATGATTCTACATTGCCCGTGGTTGAGCTAAGAGATTTAGGCAATGACGTTATTCACATAATGCTAATTAGTAGTTCCAATAGAAGCTTCATGCTCCACCAAGTTATTAGTGTCATCGAAGAGGAAGGAGGTCAAGCTGTCAACGCTAGCTTATCCATGGTTGGTAACAAGATCTTTCACTTCCTACATATCGAAGTAAGTAACGACATGGAAACATTTGAAAAGTCATTTCAAACAAATTTTAAAATAATGTCTTAGTTTCTTATTCCTCGTTTTTGTAATTTTGCAGGCTAAAATTGCGAGAGTTGGAGTAGAGACTTCAAGAGTACAACAAAGATTGCTGAATTTGGTATACCGATATTAA

mRNA sequence

ATGGCCAACGTTGGCAATGGTCAAATTTCTTCTTCTTCTTTGAAACTTGATCGCAAAACTATCGAGAAAAATAGAAGAATCCATATGAAATCTCTCTGTTCTAAACTTTTTAATCTTGTTCCTCCCTCCCATTTCAAAATCTCTAAGGAATTGTTATCGCAACAAAATCAAATCTCCTATGTAATTGCTTACATAAATGAATTGAAAGAAAGAGTGGAGAATTTGGAGAAAAGGAAACAAGCTTTACAATTAAGAAACCATTCTAATAATCATGTTCAGCAAGCTAATCTCCATAGGGTTTCGACAAGGATGATAGCTGATAATGATGATTCTACATTGCCCGTGGTTGAGCTAAGAGATTTAGGCAATGACGTTATTCACATAATGCTAATTAGTAGTTCCAATAGAAGCTTCATGCTCCACCAAGTTATTAGTGTCATCGAAGAGGAAGGAGGTCAAGCTGTCAACGCTAGCTTATCCATGGTTGGTAACAAGATCTTTCACTTCCTACATATCGAAGCTAAAATTGCGAGAGTTGGAGTAGAGACTTCAAGAGTACAACAAAGATTGCTGAATTTGGTATACCGATATTAA

Coding sequence (CDS)

ATGGCCAACGTTGGCAATGGTCAAATTTCTTCTTCTTCTTTGAAACTTGATCGCAAAACTATCGAGAAAAATAGAAGAATCCATATGAAATCTCTCTGTTCTAAACTTTTTAATCTTGTTCCTCCCTCCCATTTCAAAATCTCTAAGGAATTGTTATCGCAACAAAATCAAATCTCCTATGTAATTGCTTACATAAATGAATTGAAAGAAAGAGTGGAGAATTTGGAGAAAAGGAAACAAGCTTTACAATTAAGAAACCATTCTAATAATCATGTTCAGCAAGCTAATCTCCATAGGGTTTCGACAAGGATGATAGCTGATAATGATGATTCTACATTGCCCGTGGTTGAGCTAAGAGATTTAGGCAATGACGTTATTCACATAATGCTAATTAGTAGTTCCAATAGAAGCTTCATGCTCCACCAAGTTATTAGTGTCATCGAAGAGGAAGGAGGTCAAGCTGTCAACGCTAGCTTATCCATGGTTGGTAACAAGATCTTTCACTTCCTACATATCGAAGCTAAAATTGCGAGAGTTGGAGTAGAGACTTCAAGAGTACAACAAAGATTGCTGAATTTGGTATACCGATATTAA

Protein sequence

MANVGNGQISSSSLKLDRKTIEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYINELKERVENLEKRKQALQLRNHSNNHVQQANLHRVSTRMIADNDDSTLPVVELRDLGNDVIHIMLISSSNRSFMLHQVISVIEEEGGQAVNASLSMVGNKIFHFLHIEAKIARVGVETSRVQQRLLNLVYRY
Homology
BLAST of Bhi08G001304 vs. TAIR 10
Match: AT4G20970.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 78.2 bits (191), Expect = 8.3e-15
Identity = 60/190 (31.58%), Postives = 102/190 (53.68%), Query Frame = 0

Query: 10  SSSSLKLDRKTIEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYINELK 69
           +  S  +DRKT+EKNRR+ MKSL S+L +L+P      S E L+  +Q+     YI +L+
Sbjct: 8   TGQSRSVDRKTVEKNRRMQMKSLYSELISLLP---HHSSTEPLTLPDQLDEAANYIKKLQ 67

Query: 70  ERVENLEKRKQALQLRNHSNNHVQQANLHRVSTRMIADNDDST----LPVVELRDLGNDV 129
             VE   +RK+ L              L+ V +  ++ + D +    LP +E+++ G+ +
Sbjct: 68  VNVEKKRERKRNLVAT------TTLEKLNSVGSSSVSSSVDVSVPRKLPKIEIQETGS-I 127

Query: 130 IHIMLISSSNRSFMLHQVISVIEEE-GGQAVNASLSMVGNKIFHFLHIEAKIARVGVETS 189
            HI L++S    FM  ++I V+ EE G +  +A  S+V + +FH LH + +    G   S
Sbjct: 128 FHIFLVTSLEHKFMFCEIIRVLTEELGAEITHAGYSIVDDAVFHTLHCKVEEHDYGAR-S 186

Query: 190 RVQQRLLNLV 195
           ++ +RL  +V
Sbjct: 188 QIPERLEKIV 186

BLAST of Bhi08G001304 vs. TAIR 10
Match: AT1G10586.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.4e-14
Identity = 54/191 (28.27%), Postives = 103/191 (53.93%), Query Frame = 0

Query: 7   GQISSSSLKLDRKTIEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYIN 66
           G+ S+SSL+  R   EK RR+ MK L S L + V P+      +L+ Q       ++Y+ 
Sbjct: 8   GEGSASSLREQRNLREKERRMRMKHLFSILSSHVSPTRRLPVPQLIDQ------AVSYMI 67

Query: 67  ELKERVENLEKRKQAL---QLRNHSNNHVQQANLHRVSTRMIADNDDSTLPVVELRDLGN 126
           +LKE+V  L + K+ +   +++N S                      S LP + +R L +
Sbjct: 68  QLKEKVNYLNEMKRRMLGGEVKNRSEG-------------------SSLLPKLSIRSLDS 127

Query: 127 DVIHIMLISSSNRSFMLHQVISVIEEEGGQAVNASLSMVGNKIFHFLHIEAKIARVGVET 186
            +   +++  + +  MLH+++SV EEEG Q ++A+L  + ++ F+ +  +A I R+G++ 
Sbjct: 128 IIEMNLVMDLNMKGVMLHKLVSVFEEEGAQVMSANLQNLNDRTFYTIIAQAIICRIGIDP 173

Query: 187 SRVQQRLLNLV 195
           SR+++RL +++
Sbjct: 188 SRIEERLRDII 173

BLAST of Bhi08G001304 vs. TAIR 10
Match: AT1G10585.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 2.1e-13
Identity = 56/191 (29.32%), Postives = 103/191 (53.93%), Query Frame = 0

Query: 7   GQISSSSLKLDRKTIEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYIN 66
           G+ +SSSL+  R   EK+RR+ MK L S L + V P+        L   + I    +Y+ 
Sbjct: 8   GEGNSSSLREQRNLREKDRRMRMKHLFSILSSHVSPT------RKLPVPHLIDQATSYMI 67

Query: 67  ELKERVENLEKRKQALQLRNHSNNHVQQANLHRVSTRMIADNDDSTLPVVELRDLGNDVI 126
           +LKE V  L+++K+ L      N +     L ++S R    + DST+ +  + DL     
Sbjct: 68  QLKENVNYLKEKKRTLLQGELGNLYEGSFLLPKLSIR----SRDSTIEMNLIMDL----- 127

Query: 127 HIMLISSSNRSFMLHQVISVIEEEGGQAVNASLSMVGNKIFHFLHIEAKIARVGVETSRV 186
                  + +  MLH+++S+ EEEG Q ++A+L  + ++  + +  +A I+R+G++ SR+
Sbjct: 128 -------NMKRVMLHELVSIFEEEGAQVMSANLQNLNDRTTYTIIAQAIISRIGIDPSRI 176

Query: 187 QQRLLNLVYRY 198
           ++R+  ++Y Y
Sbjct: 188 EERVRKIIYGY 176

BLAST of Bhi08G001304 vs. TAIR 10
Match: AT1G10586.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 61.2 bits (147), Expect = 1.1e-09
Identity = 38/147 (25.85%), Postives = 79/147 (53.74%), Query Frame = 0

Query: 51  LLSQQNQISYVIAYINELKERVENLEKRKQAL---QLRNHSNNHVQQANLHRVSTRMIAD 110
           LL     I   ++Y+ +LKE+V  L + K+ +   +++N S                   
Sbjct: 5   LLPVPQLIDQAVSYMIQLKEKVNYLNEMKRRMLGGEVKNRSEG----------------- 64

Query: 111 NDDSTLPVVELRDLGNDVIHIMLISSSNRSFMLHQVISVIEEEGGQAVNASLSMVGNKIF 170
              S LP + +R L + +   +++  + +  MLH+++SV EEEG Q ++A+L  + ++ F
Sbjct: 65  --SSLLPKLSIRSLDSIIEMNLVMDLNMKGVMLHKLVSVFEEEGAQVMSANLQNLNDRTF 124

Query: 171 HFLHIEAKIARVGVETSRVQQRLLNLV 195
           + +  +A I R+G++ SR+++RL +++
Sbjct: 125 YTIIAQAIICRIGIDPSRIEERLRDII 132

BLAST of Bhi08G001304 vs. TAIR 10
Match: AT3G56970.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 46.2 bits (108), Expect = 3.5e-05
Identity = 42/162 (25.93%), Postives = 82/162 (50.62%), Query Frame = 0

Query: 15  KLDRKTIEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYINELKERVEN 74
           KL+    E++RR  + +L S L + +P S      + LS    +S  + YI EL+++V+ 
Sbjct: 73  KLNHNASERDRRKKINTLFSSLRSCLPASD---QSKKLSIPETVSKSLKYIPELQQQVKR 132

Query: 75  LEKRKQALQLRNHSNNHVQQANLHRVSTRMIADNDDSTLPVVELRDLGNDVIHIMLISSS 134
           L ++K+ + +R       +  +  +   + +A    S L  V    LG++ + + + SS 
Sbjct: 133 LIQKKEEILVRVSGQRDFELYD--KQQPKAVA----SYLSTVSATRLGDNEVMVQVSSSK 192

Query: 135 NRSFMLHQVISVIEEEGGQAVNASLS-MVGNKIFHFLHIEAK 176
             +F +  V+  IEE+G   V+ S S   G ++F+ LH++ +
Sbjct: 193 IHNFSISNVLGGIEEDGFVLVDVSSSRSQGERLFYTLHLQVE 225

BLAST of Bhi08G001304 vs. ExPASy Swiss-Prot
Match: F4JIJ7 (Transcription factor bHLH162 OS=Arabidopsis thaliana OX=3702 GN=BHLH162 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.2e-13
Identity = 60/190 (31.58%), Postives = 102/190 (53.68%), Query Frame = 0

Query: 10  SSSSLKLDRKTIEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYINELK 69
           +  S  +DRKT+EKNRR+ MKSL S+L +L+P      S E L+  +Q+     YI +L+
Sbjct: 8   TGQSRSVDRKTVEKNRRMQMKSLYSELISLLP---HHSSTEPLTLPDQLDEAANYIKKLQ 67

Query: 70  ERVENLEKRKQALQLRNHSNNHVQQANLHRVSTRMIADNDDST----LPVVELRDLGNDV 129
             VE   +RK+ L              L+ V +  ++ + D +    LP +E+++ G+ +
Sbjct: 68  VNVEKKRERKRNLVAT------TTLEKLNSVGSSSVSSSVDVSVPRKLPKIEIQETGS-I 127

Query: 130 IHIMLISSSNRSFMLHQVISVIEEE-GGQAVNASLSMVGNKIFHFLHIEAKIARVGVETS 189
            HI L++S    FM  ++I V+ EE G +  +A  S+V + +FH LH + +    G   S
Sbjct: 128 FHIFLVTSLEHKFMFCEIIRVLTEELGAEITHAGYSIVDDAVFHTLHCKVEEHDYGAR-S 186

Query: 190 RVQQRLLNLV 195
           ++ +RL  +V
Sbjct: 188 QIPERLEKIV 186

BLAST of Bhi08G001304 vs. ExPASy Swiss-Prot
Match: Q9XIJ1 (Transcription factor bHLH168 OS=Arabidopsis thaliana OX=3702 GN=BHLH168 PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.4e-13
Identity = 54/191 (28.27%), Postives = 103/191 (53.93%), Query Frame = 0

Query: 7   GQISSSSLKLDRKTIEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYIN 66
           G+ S+SSL+  R   EK RR+ MK L S L + V P+      +L+ Q       ++Y+ 
Sbjct: 8   GEGSASSLREQRNLREKERRMRMKHLFSILSSHVSPTRRLPVPQLIDQ------AVSYMI 67

Query: 67  ELKERVENLEKRKQAL---QLRNHSNNHVQQANLHRVSTRMIADNDDSTLPVVELRDLGN 126
           +LKE+V  L + K+ +   +++N S                      S LP + +R L +
Sbjct: 68  QLKEKVNYLNEMKRRMLGGEVKNRSEG-------------------SSLLPKLSIRSLDS 127

Query: 127 DVIHIMLISSSNRSFMLHQVISVIEEEGGQAVNASLSMVGNKIFHFLHIEAKIARVGVET 186
            +   +++  + +  MLH+++SV EEEG Q ++A+L  + ++ F+ +  +A I R+G++ 
Sbjct: 128 IIEMNLVMDLNMKGVMLHKLVSVFEEEGAQVMSANLQNLNDRTFYTIIAQAIICRIGIDP 173

Query: 187 SRVQQRLLNLV 195
           SR+++RL +++
Sbjct: 188 SRIEERLRDII 173

BLAST of Bhi08G001304 vs. ExPASy Swiss-Prot
Match: F4I4E1 (Transcription factor bHLH167 OS=Arabidopsis thaliana OX=3702 GN=BHLH167 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.9e-12
Identity = 56/191 (29.32%), Postives = 103/191 (53.93%), Query Frame = 0

Query: 7   GQISSSSLKLDRKTIEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYIN 66
           G+ +SSSL+  R   EK+RR+ MK L S L + V P+        L   + I    +Y+ 
Sbjct: 8   GEGNSSSLREQRNLREKDRRMRMKHLFSILSSHVSPT------RKLPVPHLIDQATSYMI 67

Query: 67  ELKERVENLEKRKQALQLRNHSNNHVQQANLHRVSTRMIADNDDSTLPVVELRDLGNDVI 126
           +LKE V  L+++K+ L      N +     L ++S R    + DST+ +  + DL     
Sbjct: 68  QLKENVNYLKEKKRTLLQGELGNLYEGSFLLPKLSIR----SRDSTIEMNLIMDL----- 127

Query: 127 HIMLISSSNRSFMLHQVISVIEEEGGQAVNASLSMVGNKIFHFLHIEAKIARVGVETSRV 186
                  + +  MLH+++S+ EEEG Q ++A+L  + ++  + +  +A I+R+G++ SR+
Sbjct: 128 -------NMKRVMLHELVSIFEEEGAQVMSANLQNLNDRTTYTIIAQAIISRIGIDPSRI 176

Query: 187 QQRLLNLVYRY 198
           ++R+  ++Y Y
Sbjct: 188 EERVRKIIYGY 176

BLAST of Bhi08G001304 vs. ExPASy Swiss-Prot
Match: Q0JFZ0 (Protein IRON-RELATED TRANSCRIPTION FACTOR 2 OS=Oryza sativa subsp. japonica OX=39947 GN=IRO2 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 3.4e-05
Identity = 52/188 (27.66%), Postives = 91/188 (48.40%), Query Frame = 0

Query: 10  SSSSLKLDRKTIEKNRRIHMKSLCSKLFNLVPPS-HFKISKELLSQQNQISYVIAYINEL 69
           S S  KL     E++RR  +  L S L  L+P + H K     LS    +S V+ YI EL
Sbjct: 65  SGSHRKLSHNAYERDRRKQLNELYSSLRALLPDADHTK-----LSIPTTVSRVLKYIPEL 124

Query: 70  KERVENLEKRKQALQLRNHSNNHVQQANLHRVSTRMIADNDDSTLPVVELRDLGNDVIHI 129
           +++VENLE++K+ L   + +N          +S  M      + +  +E+      ++ +
Sbjct: 125 QKQVENLERKKKELTTTSTTNCKPGVLGSQLMSEGMAPIVSATCINDMEI------MVQV 184

Query: 130 MLISSSNRSFM-LHQVISVIEEEGGQAVNASLSM-VGNKIFHFLHIEAKIARVGVETSRV 189
            L+S+   S + L + I V+E EG   +++S S   GN+ F+ +H++     +  E    
Sbjct: 185 SLLSNVAGSVLPLSKCIKVLENEGLHFISSSTSSGFGNRTFYSIHLQRSEGTINEECPAF 241

Query: 190 QQRLLNLV 195
            +RL  +V
Sbjct: 245 CERLEKVV 241

BLAST of Bhi08G001304 vs. ExPASy Swiss-Prot
Match: A2WZ60 (Protein IRON-RELATED TRANSCRIPTION FACTOR 2 OS=Oryza sativa subsp. indica OX=39946 GN=IRO2 PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 4.5e-05
Identity = 50/187 (26.74%), Postives = 90/187 (48.13%), Query Frame = 0

Query: 10  SSSSLKLDRKTIEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYINELK 69
           S S  KL     E++RR  +  L S L  L+P +      + LS    +S V+ YI EL+
Sbjct: 65  SGSHRKLSHNAYERDRRKQLNELYSSLRALLPDAD---HTKKLSIPTTVSRVLKYIPELQ 124

Query: 70  ERVENLEKRKQALQLRNHSNNHVQQANLHRVSTRMIADNDDSTLPVVELRDLGNDVIHIM 129
           ++VENLE++K+ L   + +N          +S  M      + +  +E+      ++ + 
Sbjct: 125 KQVENLERKKKELTTTSTTNCKPGVLGSQLMSEGMAPIVSATCINDMEI------MVQVS 184

Query: 130 LISSSNRSFM-LHQVISVIEEEGGQAVNASLSM-VGNKIFHFLHIEAKIARVGVETSRVQ 189
           L+S+   S + L + I V+E EG   +++S S   GN+ F+ +H++     +  E     
Sbjct: 185 LLSNVAGSVLPLSKCIKVLENEGLHFISSSTSSGFGNRTFYSIHLQRSEGTINEECPAFC 242

Query: 190 QRLLNLV 195
           +RL  +V
Sbjct: 245 ERLEKVV 242

BLAST of Bhi08G001304 vs. ExPASy TrEMBL
Match: A0A1S3AW43 (uncharacterized protein LOC103483440 OS=Cucumis melo OX=3656 GN=LOC103483440 PE=4 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 2.9e-68
Identity = 139/193 (72.02%), Postives = 165/193 (85.49%), Query Frame = 0

Query: 4   VGNGQISSSSLKLDRKTIEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIA 63
           VG+G  SSSS +LDRKT+EKNRR HMKSLCS LFNLVPPSHFKI KEL+SQQNQI +VIA
Sbjct: 5   VGHGGTSSSSAELDRKTMEKNRRSHMKSLCSNLFNLVPPSHFKIPKELISQQNQICHVIA 64

Query: 64  YINELKERVENLEKRKQALQLRNHSNNHVQQANLHRVSTRMIADNDDSTLPVVELRDLGN 123
           YINELKERVENLEKRKQAL+L+ HS++   QAN          +N+D TLP++E+ DLGN
Sbjct: 65  YINELKERVENLEKRKQALRLQTHSSHIHDQAN----------NNNDVTLPIIEINDLGN 124

Query: 124 DVIHIMLISSSNRSFMLHQVISVIEEEGGQAVNASLSMVGNKIFHFLHIEAKIARVGVET 183
           D++HIMLISS NRSFMLHQ+ISVIEEEGGQ VNA LS +GNK+FH LHIEAKI+R+G+ET
Sbjct: 125 DIVHIMLISSVNRSFMLHQIISVIEEEGGQVVNAGLSTIGNKVFHSLHIEAKISRIGIET 184

Query: 184 SRVQQRLLNLVYR 197
           SRV++RL+NLVY+
Sbjct: 185 SRVKRRLVNLVYQ 187

BLAST of Bhi08G001304 vs. ExPASy TrEMBL
Match: A0A6J1JY79 (transcription factor bHLH168-like OS=Cucurbita maxima OX=3661 GN=LOC111489950 PE=4 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 8.3e-55
Identity = 119/194 (61.34%), Postives = 154/194 (79.38%), Query Frame = 0

Query: 1   MANVGNGQISSSSLKLDRKTIEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISY 60
           MA  GN     +S KLDRKT+EKNRRIHMKSLCSKLF+LVP SH+KISK+LLSQQ QISY
Sbjct: 30  MAKPGNN--GGASFKLDRKTVEKNRRIHMKSLCSKLFDLVPSSHYKISKDLLSQQTQISY 89

Query: 61  VIAYINELKERVENLEKRKQALQLRNHSNNHVQQANLHRVSTRMIADNDDSTLPVVELRD 120
           VIAYINELKERVE+LEKRK+ALQL+ HS                ++ ++DST P+VE+++
Sbjct: 90  VIAYINELKERVESLEKRKEALQLQIHS---------------QLSTSNDSTFPIVEVKE 149

Query: 121 LGNDVIHIMLISSSNRSFMLHQVISVIEEEGGQAVNASLSMVGNKIFHFLHIEAKIARVG 180
           L + ++H+ LISS +R+ ML+++I VIEEEGG+ V+AS S +G+KIFH LHIEAKI+RVG
Sbjct: 150 LCSGIVHVTLISSVHRNVMLNEIIVVIEEEGGEVVSASFSTLGDKIFHSLHIEAKISRVG 206

Query: 181 VETSRVQQRLLNLV 195
           +ETSR+++RL NL+
Sbjct: 210 IETSRIEKRLENLI 206

BLAST of Bhi08G001304 vs. ExPASy TrEMBL
Match: A0A6J1GQZ4 (transcription factor bHLH168-like OS=Cucurbita moschata OX=3662 GN=LOC111456691 PE=4 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 2.7e-53
Identity = 113/183 (61.75%), Postives = 147/183 (80.33%), Query Frame = 0

Query: 12  SSLKLDRKTIEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYINELKER 71
           +S+KLDRK +EKNRRIHMKSLCSKLFNLVP SH+KISK+LLSQQ QISYVIAYI ELKER
Sbjct: 10  ASIKLDRKIVEKNRRIHMKSLCSKLFNLVPSSHYKISKDLLSQQTQISYVIAYIKELKER 69

Query: 72  VENLEKRKQALQLRNHSNNHVQQANLHRVSTRMIADNDDSTLPVVELRDLGNDVIHIMLI 131
           VE+LEKRK+ALQL+ HS                +  + DST P+VE+R+L + ++H+ LI
Sbjct: 70  VESLEKRKEALQLQIHS---------------QLPTSSDSTFPIVEVRELCSGIVHVTLI 129

Query: 132 SSSNRSFMLHQVISVIEEEGGQAVNASLSMVGNKIFHFLHIEAKIARVGVETSRVQQRLL 191
           SS +R+ ML+++I V+EEEGG+ V+AS S +G+K+FH LHIEAKI+RVG+ETSRV++RL 
Sbjct: 130 SSVDRNVMLNEIIGVVEEEGGEVVSASFSTLGDKVFHSLHIEAKISRVGIETSRVEKRLK 177

Query: 192 NLV 195
           +L+
Sbjct: 190 HLI 177

BLAST of Bhi08G001304 vs. ExPASy TrEMBL
Match: A0A6J1C669 (transcription factor bHLH162-like OS=Momordica charantia OX=3673 GN=LOC111008798 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 8.6e-44
Identity = 103/157 (65.61%), Postives = 124/157 (78.98%), Query Frame = 0

Query: 10  SSSSLKLDRKTIEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYINELK 69
           + +SLKLDRKTIEKNRR+HMKSLCSKL +L+PPSHFKISK+LLSQQNQISYVIAYINELK
Sbjct: 7   AETSLKLDRKTIEKNRRVHMKSLCSKLVSLIPPSHFKISKDLLSQQNQISYVIAYINELK 66

Query: 70  ERVENLEKRKQALQLRNHSNNHVQQANLHRVSTRMIADNDDSTLPVVELRDLGNDVIHIM 129
           ERVE LEK+K+AL+  +H ++H    +              STLPVVELRDLG+ VI +M
Sbjct: 67  ERVEKLEKKKRALEFTHHHHHHQASRS---------PGGGGSTLPVVELRDLGS-VIEVM 126

Query: 130 LISSSNRSFMLHQVISVIEEEGGQAVNASLSMVGNKI 167
           LIS  NR+F+L QVI+++EEEGGQ VNAS S +G KI
Sbjct: 127 LISDLNRNFLLCQVIAIVEEEGGQVVNASFSTLGGKI 153

BLAST of Bhi08G001304 vs. ExPASy TrEMBL
Match: A0A067KH05 (BHLH domain-containing protein OS=Jatropha curcas OX=180498 GN=JCGZ_11490 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 6.2e-42
Identity = 97/186 (52.15%), Postives = 133/186 (71.51%), Query Frame = 0

Query: 10  SSSSLKLDRKTIEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYINELK 69
           S  SLKLDRKT+EKNRRIHMK+LC KL +++P  HFK  K++LSQQ+Q+ +  AYI  LK
Sbjct: 5   SRDSLKLDRKTVEKNRRIHMKALCFKLASVIPSQHFKHHKDMLSQQDQLDHAAAYIKNLK 64

Query: 70  ERVENLEKRKQALQLRNHSNNHVQQANLHRVSTRMIADNDDSTLPVVELRDLGNDVIHIM 129
           ER+E LEK+K+       +   + +AN +     M+      TLP+ ELRDLGN  I ++
Sbjct: 65  ERIEKLEKKKE------RTLGSLSEANNNNAMNEMMM---SLTLPMFELRDLGNS-IEVV 124

Query: 130 LISSSNRSFMLHQVISVIEEEGGQAVNASLSMVGNKIFHFLHIEAKIARVGVETSRVQQR 189
           LIS S R+FMLH+VI+++EEEG + V+AS + VG+K+FH +H + KI+RVGVET+RV QR
Sbjct: 125 LISGSKRNFMLHEVITILEEEGAEVVSASYTTVGDKVFHTIHAQVKISRVGVETTRVCQR 180

Query: 190 LLNLVY 196
           L  L+Y
Sbjct: 185 LQELIY 180

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G20970.18.3e-1531.58basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G10586.12.4e-1428.27basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G10585.12.1e-1329.32basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G10586.21.1e-0925.85basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT3G56970.13.5e-0525.93basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
Match NameE-valueIdentityDescription
F4JIJ71.2e-1331.58Transcription factor bHLH162 OS=Arabidopsis thaliana OX=3702 GN=BHLH162 PE=1 SV=... [more]
Q9XIJ13.4e-1328.27Transcription factor bHLH168 OS=Arabidopsis thaliana OX=3702 GN=BHLH168 PE=3 SV=... [more]
F4I4E12.9e-1229.32Transcription factor bHLH167 OS=Arabidopsis thaliana OX=3702 GN=BHLH167 PE=2 SV=... [more]
Q0JFZ03.4e-0527.66Protein IRON-RELATED TRANSCRIPTION FACTOR 2 OS=Oryza sativa subsp. japonica OX=3... [more]
A2WZ604.5e-0526.74Protein IRON-RELATED TRANSCRIPTION FACTOR 2 OS=Oryza sativa subsp. indica OX=399... [more]
Match NameE-valueIdentityDescription
A0A1S3AW432.9e-6872.02uncharacterized protein LOC103483440 OS=Cucumis melo OX=3656 GN=LOC103483440 PE=... [more]
A0A6J1JY798.3e-5561.34transcription factor bHLH168-like OS=Cucurbita maxima OX=3661 GN=LOC111489950 PE... [more]
A0A6J1GQZ42.7e-5361.75transcription factor bHLH168-like OS=Cucurbita moschata OX=3662 GN=LOC111456691 ... [more]
A0A6J1C6698.6e-4465.61transcription factor bHLH162-like OS=Momordica charantia OX=3673 GN=LOC111008798... [more]
A0A067KH056.2e-4252.15BHLH domain-containing protein OS=Jatropha curcas OX=180498 GN=JCGZ_11490 PE=4 S... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 65..85
NoneNo IPR availablePANTHERPTHR13935:SF52OS01G0108400 PROTEINcoord: 13..193
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 13..98
e-value: 2.8E-8
score: 35.8
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 18..88
IPR015660Achaete-scute transcription factor-relatedPANTHERPTHR13935ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATEDcoord: 13..193

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M001304Bhi08M001304mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:2000112 regulation of cellular macromolecule biosynthetic process
biological_process GO:0006357 regulation of transcription by RNA polymerase II
cellular_component GO:0090575 RNA polymerase II transcription regulator complex
molecular_function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity