Bhi08G001231 (gene) Wax gourd (B227) v1

Overview
NameBhi08G001231
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionglucuronoxylan 4-O-methyltransferase 1
Locationchr8: 43334221 .. 43335168 (-)
RNA-Seq ExpressionBhi08G001231
SyntenyBhi08G001231
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCCCGAATTGGTGGATTGCCACTCACTTGTGCCATCAGAATCGCTTTCTCCCCCCAGTATCTCACCCTCACAGCAGTCATATCCATCAGTAAGCTTCAGAAACAGTAGAGGAAGCAAAAGCATGAGTTTTACAAGAAACAAAATCCTACCTGTTCTCGTCTTGATTCTGTCTAGTCTGTCCATTCTCAGACTCGTTAGGATTGCACTTACCACATCTTATACTTCTCCACTTCTAGCAATTGCATTGCCTCCAAAGCTTCAACGCCCATTATCAGGGTTCTCAGAAAATCATAGAACCTCTGCAAATGAAGCCGCTATTTCAGAGACGGAATTCAAGCTTCTTTCAAATCTGATTGGTGAAAAGGCACCCTGCAATCTCCTCTTCTTTGGGATCGAACCACAGTACCTTAACCTCTCAGCAGTCAATGACGGTGGAAAGAATGTTTTTCTTGAGGATGATCCCTACAAACTGAGCACATTCAGAGTCGACTCAAATCACACACGAATGTACAAAATTGATTATAAGTTTCATGCAGAAAAAGCTTACAAGTTATTAAAGCAAGCAAGAGGAAACAAATCATGTGCACCAGACTCAGGGCTCAGGAATTCATCTAAGTGCAAACTTGCCTTGACAGATCTTCCAAAAGAAGTTTATGAAGTCAAATGGGATGTGGTGGTCGTGGATGGACCAAGGGGAGATAGTCCTCATGCACCAGGAAGAATGGCAACAATTTACAGTGCTGCTATCATGGCAAGAGAAGGGAACACCACTGATGTAGTTGTACATGATACAGACCGTATGATCGAAAGGTGGTATTCTTGGGAGTTCCTTTGTGATGAAAACTTGATTTCCTCCAAAGGGAAGCTCTGGATTTTCAGGATTAGAAATGAGAAAAATTCATCCAGATTCTGCCCTGACAAACGCATTGTGGTCCAACGATGA

mRNA sequence

ATGCCTCCCGAATTGGTGGATTGCCACTCACTTGTGCCATCAGAATCGCTTTCTCCCCCCAGTATCTCACCCTCACAGCAGTCATATCCATCAGTAAGCTTCAGAAACAGTAGAGGAAGCAAAAGCATGAGTTTTACAAGAAACAAAATCCTACCTGTTCTCGTCTTGATTCTGTCTAGTCTGTCCATTCTCAGACTCGTTAGGATTGCACTTACCACATCTTATACTTCTCCACTTCTAGCAATTGCATTGCCTCCAAAGCTTCAACGCCCATTATCAGGGTTCTCAGAAAATCATAGAACCTCTGCAAATGAAGCCGCTATTTCAGAGACGGAATTCAAGCTTCTTTCAAATCTGATTGGTGAAAAGGCACCCTGCAATCTCCTCTTCTTTGGGATCGAACCACAGTACCTTAACCTCTCAGCAGTCAATGACGGTGGAAAGAATGTTTTTCTTGAGGATGATCCCTACAAACTGAGCACATTCAGAGTCGACTCAAATCACACACGAATGTACAAAATTGATTATAAGTTTCATGCAGAAAAAGCTTACAAGTTATTAAAGCAAGCAAGAGGAAACAAATCATGTGCACCAGACTCAGGGCTCAGGAATTCATCTAAGTGCAAACTTGCCTTGACAGATCTTCCAAAAGAAGTTTATGAAGTCAAATGGGATGTGGTGGTCGTGGATGGACCAAGGGGAGATAGTCCTCATGCACCAGGAAGAATGGCAACAATTTACAGTGCTGCTATCATGGCAAGAGAAGGGAACACCACTGATGTAGTTGTACATGATACAGACCGTATGATCGAAAGGTGGTATTCTTGGGAGTTCCTTTGTGATGAAAACTTGATTTCCTCCAAAGGGAAGCTCTGGATTTTCAGGATTAGAAATGAGAAAAATTCATCCAGATTCTGCCCTGACAAACGCATTGTGGTCCAACGATGA

Coding sequence (CDS)

ATGCCTCCCGAATTGGTGGATTGCCACTCACTTGTGCCATCAGAATCGCTTTCTCCCCCCAGTATCTCACCCTCACAGCAGTCATATCCATCAGTAAGCTTCAGAAACAGTAGAGGAAGCAAAAGCATGAGTTTTACAAGAAACAAAATCCTACCTGTTCTCGTCTTGATTCTGTCTAGTCTGTCCATTCTCAGACTCGTTAGGATTGCACTTACCACATCTTATACTTCTCCACTTCTAGCAATTGCATTGCCTCCAAAGCTTCAACGCCCATTATCAGGGTTCTCAGAAAATCATAGAACCTCTGCAAATGAAGCCGCTATTTCAGAGACGGAATTCAAGCTTCTTTCAAATCTGATTGGTGAAAAGGCACCCTGCAATCTCCTCTTCTTTGGGATCGAACCACAGTACCTTAACCTCTCAGCAGTCAATGACGGTGGAAAGAATGTTTTTCTTGAGGATGATCCCTACAAACTGAGCACATTCAGAGTCGACTCAAATCACACACGAATGTACAAAATTGATTATAAGTTTCATGCAGAAAAAGCTTACAAGTTATTAAAGCAAGCAAGAGGAAACAAATCATGTGCACCAGACTCAGGGCTCAGGAATTCATCTAAGTGCAAACTTGCCTTGACAGATCTTCCAAAAGAAGTTTATGAAGTCAAATGGGATGTGGTGGTCGTGGATGGACCAAGGGGAGATAGTCCTCATGCACCAGGAAGAATGGCAACAATTTACAGTGCTGCTATCATGGCAAGAGAAGGGAACACCACTGATGTAGTTGTACATGATACAGACCGTATGATCGAAAGGTGGTATTCTTGGGAGTTCCTTTGTGATGAAAACTTGATTTCCTCCAAAGGGAAGCTCTGGATTTTCAGGATTAGAAATGAGAAAAATTCATCCAGATTCTGCCCTGACAAACGCATTGTGGTCCAACGATGA

Protein sequence

MPPELVDCHSLVPSESLSPPSISPSQQSYPSVSFRNSRGSKSMSFTRNKILPVLVLILSSLSILRLVRIALTTSYTSPLLAIALPPKLQRPLSGFSENHRTSANEAAISETEFKLLSNLIGEKAPCNLLFFGIEPQYLNLSAVNDGGKNVFLEDDPYKLSTFRVDSNHTRMYKIDYKFHAEKAYKLLKQARGNKSCAPDSGLRNSSKCKLALTDLPKEVYEVKWDVVVVDGPRGDSPHAPGRMATIYSAAIMAREGNTTDVVVHDTDRMIERWYSWEFLCDENLISSKGKLWIFRIRNEKNSSRFCPDKRIVVQR
Homology
BLAST of Bhi08G001231 vs. TAIR 10
Match: AT4G24910.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 245.0 bits (624), Expect = 8.2e-65
Identity = 141/327 (43.12%), Postives = 198/327 (60.55%), Query Frame = 0

Query: 1   MPPEL--VDCHSLVPSESLSPPSISPSQQSYPSVSFRNSRGSKSMSFTRNKILPVLVLIL 60
           MPP+        L P    SPPS SP    +  +S       K   FT  K++P+L+ +L
Sbjct: 1   MPPQFHQSSLSILNPLLRFSPPS-SPDNPKHQRLS-----TIKMPKFTVRKLIPLLIFVL 60

Query: 61  SSLSILRLVRIAL---TTSYTSPLLAIALPPKLQRPLSGFSENHRTSANEAAISETEFKL 120
           SSLS+LRL+RI+    + S +S      L P      +  S+  R +    A+ E E KL
Sbjct: 61  SSLSVLRLLRISYKSSSKSQSSSSTTFRLSP------ADSSQQLRANEGPTALMEKELKL 120

Query: 121 LSNLIGEKAPCNLLFFGIEPQYLNLSAVNDGGKNVFLEDDPYK--LSTFRVDSNHTRMYK 180
           LS+ +  ++PCN+L FG  PQYL LS++N  G  V LED+P K  +    V+ N+TR+Y 
Sbjct: 121 LSDTVTRRSPCNILVFGFAPQYLMLSSINTRGITVILEDEPAKIMIPKAEVNPNNTRIYS 180

Query: 181 IDY-KFHAEKAYKLLKQARGNKSCAPDSGLRN--SSKCKLALTDLPKEVYEVKWDVVVVD 240
           + Y +     AY LL+ AR N +CAP+   ++  SS CKL L DLP++V+  KWDV+VVD
Sbjct: 181 LKYHQMEVRNAYNLLQHARANPACAPNMNNQHQGSSDCKLELRDLPQQVHNTKWDVIVVD 240

Query: 241 GPRGDSPHAPGRMATIYSAAIMAREG---NTTDVVVHDTDRMIERWYSWEFLCDENLISS 300
           GPRGD    PGRM +IY+AA++AR+G   +TTDV VHD  R  E+W SWEFLC ENL+S+
Sbjct: 241 GPRGDDLETPGRMGSIYTAAVLARKGSSNSTTDVFVHDVHRTAEKWLSWEFLCQENLVSA 300

Query: 301 KGKLWIFRIRNEKNSSRFCPDKRIVVQ 315
           KG  W FRI+ + N+SRFC  + ++++
Sbjct: 301 KGTFWKFRIKRQSNASRFCSPETVIIR 315

BLAST of Bhi08G001231 vs. TAIR 10
Match: AT1G67330.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 154.8 bits (390), Expect = 1.1e-37
Identity = 100/286 (34.97%), Postives = 148/286 (51.75%), Query Frame = 0

Query: 37  SRGSKSMSFTRNKILPVLV--LILSSLSILRLVRI---ALTTSYTSPLLAIALPPKLQRP 96
           S+G+K     R   L V +  LI  ++ I   +R     L+   T+   A ++      P
Sbjct: 6   SKGAKQTLLERPWFLAVALAGLIGGAMLITSFIRATDNTLSLCSTAKNTAASIAKYTATP 65

Query: 97  LSGFSENH-RTSANEAAISETEFKLLSNLIGEKAPCNLLFFGIEPQYLNLSAVNDGGKNV 156
           +   S  H  TS      S  E  +  N++ E+ PCN L FG+    L  +++N GG  V
Sbjct: 66  IQLQSIVHYATSHTVPQQSFEEISISLNVLKERLPCNFLVFGLGRDSLMWASLNPGGTTV 125

Query: 157 FLEDDPYKLSTFRVDSNHTRMYKIDYKFHAEKAYKLLKQARGNKSCAPDSG--LRNSSKC 216
           FLE+DP  +     D+   R + + Y+ H  +A +LL   +    C P     +R + KC
Sbjct: 126 FLEEDPEWIEAVLKDAPSLRAHHVQYRTHLSEAGRLLSTYKNEPMCLPAKAFPIRYNEKC 185

Query: 217 KLALTDLPKEVYEVKWDVVVVDGPRGDSPHAPGRMATIYSAAIMARE---GNTTDVVVHD 276
            LALT LP E Y+ +WD+++VD P+G  P APGRMA I+S+AIMAR      TT V +HD
Sbjct: 186 PLALTSLPDEFYDTEWDLIMVDAPKGYFPEAPGRMAAIFSSAIMARNRKGDGTTHVFLHD 245

Query: 277 TDRMIERWYSWEFLCDENLISSKGKLWIFRIRNEKN-----SSRFC 307
            +R +E  ++ EFLC++  ++S G+LW F I N  N       RFC
Sbjct: 246 VNRKVENAFANEFLCEKYKVNSVGRLWHFEIPNAANMTDQPGDRFC 291

BLAST of Bhi08G001231 vs. TAIR 10
Match: AT2G15440.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 147.9 bits (372), Expect = 1.4e-35
Identity = 88/244 (36.07%), Postives = 131/244 (53.69%), Query Frame = 0

Query: 74  SYTSPLLAIALPPKLQRPLSGFSENHRTSANEAAISETEFKLLSNLIGEKAP-CNLLFFG 133
           S +S   + +LPP +   L  ++    +S    ++S  E   +SN+I    P CNLL FG
Sbjct: 64  SLSSSPSSSSLPPPVLAALLHYTS---SSPPNTSMSFLELSTISNIIHSHGPACNLLIFG 123

Query: 134 IEPQYLNLSAVNDGGKNVFLEDDPYKLSTFRVDSNHTRMYKIDYKFHAEKAYKLLKQARG 193
           +  + L   ++N  G+ VF+++ PY +S F   +     Y++ Y     +A KLL   + 
Sbjct: 124 LTHESLLWRSINFQGRTVFVDESPYSVSKFEQSNPGVEAYEVVYSTKVSQAKKLLGYYKT 183

Query: 194 NKSCAPDSGLRNSSKCKLALTDLPKEVYEVKWDVVVVDGPRGDSPHAPGRMATIYSAAIM 253
              C P   L   S CKL + DLP  VYE+ WDV+++DGPRG +  +PGRMA I+++A++
Sbjct: 184 RPECRPVQNLL-FSDCKLGINDLPNFVYEIDWDVILIDGPRGYASDSPGRMAPIFTSAVL 243

Query: 254 AR------EGNTTDVVVHDTDRMIERWYSWEFLCDENLISSKGKLWIFRI----RNEKNS 307
           A+      +   TDV+VH+  R IER YS EFLC+ENLI   G L  F +      E   
Sbjct: 244 AKSKDFGTKTKKTDVLVHEFGRKIERVYSEEFLCEENLIEVVGDLGHFVVAAAEERESYG 303

BLAST of Bhi08G001231 vs. TAIR 10
Match: AT1G27930.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 140.6 bits (353), Expect = 2.2e-33
Identity = 73/189 (38.62%), Postives = 111/189 (58.73%), Query Frame = 0

Query: 112 EFKLLSNLIGEKAPCNLLFFGIEPQYLNLSAVNDGGKNVFLEDDPYKLSTFRVDSNHTRM 171
           E  +  N++ + AP N L FG+    L  +++N  GK +FLE+D         DS   R 
Sbjct: 84  EISISFNILKKLAPANFLVFGLGRDSLMWASLNPRGKTLFLEEDLEWFQKVTKDSPFLRA 143

Query: 172 YKIDYKFHAEKAYKLLKQARGNKSCAP-DSGLRNSSKCKLALTDLPKEVYEVKWDVVVVD 231
           + + Y+   ++A  LL+  +    C P  S LR + KCKLALT LP E Y+ +WD+++VD
Sbjct: 144 HHVRYRTQLQQADSLLRSYKTEPKCFPAKSYLRGNEKCKLALTGLPDEFYDTEWDLLMVD 203

Query: 232 GPRGDSPHAPGRMATIYSAAIMAR---EGNTTDVVVHDTDRMIERWYSWEFLCDENLISS 291
            P+G    APGRMA I+SAA+MAR   +   T V +HD +R +E+ ++ EFLC +  +++
Sbjct: 204 APKGYFAEAPGRMAAIFSAAVMARNRKKPGVTHVFLHDVNRRVEKTFAEEFLCRKYRVNA 263

Query: 292 KGKLWIFRI 297
            G+LW F I
Sbjct: 264 AGRLWHFAI 272

BLAST of Bhi08G001231 vs. TAIR 10
Match: AT1G09610.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 136.7 bits (343), Expect = 3.2e-32
Identity = 95/276 (34.42%), Postives = 143/276 (51.81%), Query Frame = 0

Query: 48  NKILPVLVLILSSLSILRLVRIALTTSY-------TSPLLAIALPPKLQRPLSGFSENHR 107
           +K+  +LV +L++L ++ +VR  LT+S        T          KL R L+    ++ 
Sbjct: 9   HKLKVLLVFLLATLILIFIVRSTLTSSQEHQTPQETRSTRCSGACNKLPRSLAQALIHYS 68

Query: 108 TSANEAAISETEFKLLSNLIGEKAPCNLLFFGIEPQYLNLSAVNDGGKNVFLEDDPYKLS 167
           TS      +  E  + S ++G+K+PCN L FG+    L  S++N GG+ VFLE+D   + 
Sbjct: 69  TSVITPQQTLKEIAVSSRVLGKKSPCNFLVFGLGHDSLMWSSLNYGGRTVFLEEDEAWIK 128

Query: 168 TFRVDSNHTRMYKIDYKFHAEKAYKLLKQARGNKSCAPDSGLRNSSKCKLALTDLPKEVY 227
             +        Y + Y     +A  L++  +G +  A   G    S C+LAL  LP E+Y
Sbjct: 129 QIKRRFPMLESYHVTYDSKVNQADNLIEVGKGPECTA--IGDPRYSMCQLALKGLPAEIY 188

Query: 228 EVKWDVVVVDGPRGDSPHAPGRMATIYSAAIMAR---EGNTTDVVVHDTDRMIERWYSWE 287
           E  WD+++VD P G    APGRM  IY+A +MAR   +G  TDV VHD +R IE  +S  
Sbjct: 189 ETGWDLIMVDAPTGYYDEAPGRMTAIYTAGMMARNRKQGGETDVFVHDVNREIEDKFSKA 248

Query: 288 FLCDENLISSKGKLWIF-----RIRNEKNSSR-FCP 308
           FLC+  +   +G+L  F     R  +E  S+R FCP
Sbjct: 249 FLCEGYMKKQEGRLRHFIIPSYRDGSESESNRPFCP 282

BLAST of Bhi08G001231 vs. ExPASy Swiss-Prot
Match: Q9SNE5 (Protein IRREGULAR XYLEM 15 OS=Arabidopsis thaliana OX=3702 GN=IRX15 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 3.6e-33
Identity = 93/248 (37.50%), Postives = 140/248 (56.45%), Query Frame = 0

Query: 72  TTSYTSPLLAIALPPKLQRPLSGFSENHRTSANEAAISETEFKLLSNLIGEKA-PCNLLF 131
           T S +SP+    LP      L  ++     S +   +S  E K +S+++   A PCNLL 
Sbjct: 72  TPSASSPISNSNLPTSAINALLHYASRSNDSFH---MSYGEMKSISDVLRRCAPPCNLLV 131

Query: 132 FGIEPQYLNLSAVNDGGKNVFLEDDPYKLSTFRVDSNHTRMYKIDYKFHAEKAYKLL--- 191
           FG+  + L   ++N  G+ VF+E++ Y  + F        ++ + Y   A +A +L+   
Sbjct: 132 FGLTHETLLWKSLNHNGRTVFIEENRYYAAYFEEIHPEIDVFDVQYTTKAHEAGELVTAA 191

Query: 192 KQARGNKSCAPDSGLRNSSKCKLALTDLPKEVYEVKWDVVVVDGPRGDSPHAPGRMATIY 251
           K+A GN+ C P   L   S CKL L DLP  VY+V WDV+ VDGPRGD+   PGRM++I+
Sbjct: 192 KEAAGNE-CRPVQNLL-FSDCKLGLNDLPNHVYDVDWDVIFVDGPRGDAHEGPGRMSSIF 251

Query: 252 SAAIMAR--EGNT--TDVVVHDTDRMIERWYSWEFLCDENLISSKGKLWIFRI-RNEKNS 311
           +AA++AR  +G T  T V VHD  R +ER    EFLC ENL+ S   L  + + + +KNS
Sbjct: 252 TAAVLARSKKGGTPKTHVFVHDYYRDVERLCGDEFLCRENLVESNDLLAHYVLDKMDKNS 311

BLAST of Bhi08G001231 vs. ExPASy Swiss-Prot
Match: Q9C7F9 (Probable methyltransferase At1g27930 OS=Arabidopsis thaliana OX=3702 GN=At1g27930 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 3.1e-32
Identity = 73/189 (38.62%), Postives = 111/189 (58.73%), Query Frame = 0

Query: 112 EFKLLSNLIGEKAPCNLLFFGIEPQYLNLSAVNDGGKNVFLEDDPYKLSTFRVDSNHTRM 171
           E  +  N++ + AP N L FG+    L  +++N  GK +FLE+D         DS   R 
Sbjct: 84  EISISFNILKKLAPANFLVFGLGRDSLMWASLNPRGKTLFLEEDLEWFQKVTKDSPFLRA 143

Query: 172 YKIDYKFHAEKAYKLLKQARGNKSCAP-DSGLRNSSKCKLALTDLPKEVYEVKWDVVVVD 231
           + + Y+   ++A  LL+  +    C P  S LR + KCKLALT LP E Y+ +WD+++VD
Sbjct: 144 HHVRYRTQLQQADSLLRSYKTEPKCFPAKSYLRGNEKCKLALTGLPDEFYDTEWDLLMVD 203

Query: 232 GPRGDSPHAPGRMATIYSAAIMAR---EGNTTDVVVHDTDRMIERWYSWEFLCDENLISS 291
            P+G    APGRMA I+SAA+MAR   +   T V +HD +R +E+ ++ EFLC +  +++
Sbjct: 204 APKGYFAEAPGRMAAIFSAAVMARNRKKPGVTHVFLHDVNRRVEKTFAEEFLCRKYRVNA 263

Query: 292 KGKLWIFRI 297
            G+LW F I
Sbjct: 264 AGRLWHFAI 272

BLAST of Bhi08G001231 vs. ExPASy Swiss-Prot
Match: Q6NMK1 (Glucuronoxylan 4-O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=GXM1 PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 4.4e-31
Identity = 95/276 (34.42%), Postives = 143/276 (51.81%), Query Frame = 0

Query: 48  NKILPVLVLILSSLSILRLVRIALTTSY-------TSPLLAIALPPKLQRPLSGFSENHR 107
           +K+  +LV +L++L ++ +VR  LT+S        T          KL R L+    ++ 
Sbjct: 9   HKLKVLLVFLLATLILIFIVRSTLTSSQEHQTPQETRSTRCSGACNKLPRSLAQALIHYS 68

Query: 108 TSANEAAISETEFKLLSNLIGEKAPCNLLFFGIEPQYLNLSAVNDGGKNVFLEDDPYKLS 167
           TS      +  E  + S ++G+K+PCN L FG+    L  S++N GG+ VFLE+D   + 
Sbjct: 69  TSVITPQQTLKEIAVSSRVLGKKSPCNFLVFGLGHDSLMWSSLNYGGRTVFLEEDEAWIK 128

Query: 168 TFRVDSNHTRMYKIDYKFHAEKAYKLLKQARGNKSCAPDSGLRNSSKCKLALTDLPKEVY 227
             +        Y + Y     +A  L++  +G +  A   G    S C+LAL  LP E+Y
Sbjct: 129 QIKRRFPMLESYHVTYDSKVNQADNLIEVGKGPECTA--IGDPRYSMCQLALKGLPAEIY 188

Query: 228 EVKWDVVVVDGPRGDSPHAPGRMATIYSAAIMAR---EGNTTDVVVHDTDRMIERWYSWE 287
           E  WD+++VD P G    APGRM  IY+A +MAR   +G  TDV VHD +R IE  +S  
Sbjct: 189 ETGWDLIMVDAPTGYYDEAPGRMTAIYTAGMMARNRKQGGETDVFVHDVNREIEDKFSKA 248

Query: 288 FLCDENLISSKGKLWIF-----RIRNEKNSSR-FCP 308
           FLC+  +   +G+L  F     R  +E  S+R FCP
Sbjct: 249 FLCEGYMKKQEGRLRHFIIPSYRDGSESESNRPFCP 282

BLAST of Bhi08G001231 vs. ExPASy Swiss-Prot
Match: Q9FH92 (Protein IRX15-LIKE OS=Arabidopsis thaliana OX=3702 GN=IRX15-L PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 5.8e-31
Identity = 93/262 (35.50%), Postives = 146/262 (55.73%), Query Frame = 0

Query: 59  SSLSILRLVRIALTTSYTSPLLAIALPPKLQRPLSGFSENHRTSANEA-AISETEFKLLS 118
           +S ++   V  A+TT+  S L   A+   L         ++ + +N++  +S  E K +S
Sbjct: 57  NSATVSSAVNSAVTTATISQLPTTAINAML---------HYASRSNDSYHMSYGEMKSIS 116

Query: 119 NLIGE-KAPCNLLFFGIEPQYLNLSAVNDGGKNVFLEDDPYKLSTFRVDSNHTRMYKIDY 178
           +++     PCNLL FG+  + L   ++N  G+ VF+E++ Y  + F        ++ + Y
Sbjct: 117 DVLRRCSPPCNLLVFGLTHETLLWKSLNHNGRTVFIEENRYYAAYFEEIHPEIEVFDVQY 176

Query: 179 KFHAEKAYKL---LKQARGNKSCAPDSGLRNSSKCKLALTDLPKEVYEVKWDVVVVDGPR 238
              A +A +L   +K+A  N+ C P   L   S CKL L DLP  VY+V WDV++VDGPR
Sbjct: 177 TTKAREARELVSAVKEAARNE-CRPVQNLL-FSDCKLGLNDLPNHVYDVDWDVILVDGPR 236

Query: 239 GDSPHAPGRMATIYSAAIMARE---GN-TTDVVVHDTDRMIERWYSWEFLCDENLISSKG 298
           GD    PGRM++I++AA++AR    GN  T V VHD  R +ER    EFLC ENL+ S  
Sbjct: 237 GDGGDVPGRMSSIFTAAVLARSKKGGNPKTHVFVHDYYRDVERLCGDEFLCRENLVESND 296

Query: 299 KLWIFRI-RNEKNSSRFCPDKR 311
            L  + + + +KNS++FC  ++
Sbjct: 297 LLAHYVLEKMDKNSTQFCRGRK 307

BLAST of Bhi08G001231 vs. ExPASy Swiss-Prot
Match: Q9LQ32 (Glucuronoxylan 4-O-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=GXM3 PE=1 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.7e-28
Identity = 71/200 (35.50%), Postives = 110/200 (55.00%), Query Frame = 0

Query: 112 EFKLLSNLIGEKAPCNLLFFGIEPQYLNLSAVNDGGKNVFLEDDPYKLSTFRVDSNHTRM 171
           E  +   ++ +K+PCN L FG+    L  +++N GG+ +F+E+D   ++       +   
Sbjct: 93  EVSVSKRVLDKKSPCNFLVFGLGHDSLMWASLNHGGRTLFIEEDQAWIAIVTKKFPNLES 152

Query: 172 YKIDYKFHAEKAYKLLKQARGNKSCAPDSGLRNSSKCKLALTDLPKEVYEVKWDVVVVDG 231
           Y + Y    + + KL++  R ++ C   S  RN SKC LAL D P + YE KWD+++VD 
Sbjct: 153 YHVVYDTKVKDSDKLMELGR-SEECRSVSDPRN-SKCDLALKDFPADFYETKWDLIMVDA 212

Query: 232 PRGDSPHAPGRMATIYSAAIMA--REGNTTDVVVHDTDRMIERWYSWEFLCDENLISSKG 291
           P G    APGRM+ IY+A ++A  RE   TDV VHD +R +E  +S  FLC   +    G
Sbjct: 213 PTGYHEEAPGRMSAIYTAGLLARNREDGETDVFVHDVNRPVEDEFSATFLCKGYMREQNG 272

Query: 292 KLWIFRIRNEKNSS--RFCP 308
           +L  F I + +  +   FCP
Sbjct: 273 RLRHFTIPSHRARAGRPFCP 290

BLAST of Bhi08G001231 vs. ExPASy TrEMBL
Match: A0A5A7UNX6 (Protein IRX15-LIKE OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001790 PE=4 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.5e-154
Identity = 281/312 (90.06%), Postives = 293/312 (93.91%), Query Frame = 0

Query: 1   MPPELVDCHSLVPSESLSPPSISPSQQSYPSVSFRNSRGSKSMSFTRNKILPVLVLILSS 60
           M P + DCHSLVPSE+LSPPSISPSQQS PSVSF NS+GSK+MSFTRNK+LPVLVLILSS
Sbjct: 1   MLPAVEDCHSLVPSEALSPPSISPSQQSIPSVSFTNSKGSKNMSFTRNKLLPVLVLILSS 60

Query: 61  LSILRLVRIALTTSYTSPLLAIALPPKLQRPLSGFSENH-RTSANEAAISETEFKLLSNL 120
           LSILRLVRIALTTS TSPLLAIALPPKLQRP SGFSE H RTSANEAAIS  EFKLLSNL
Sbjct: 61  LSILRLVRIALTTSNTSPLLAIALPPKLQRPKSGFSEKHNRTSANEAAISGKEFKLLSNL 120

Query: 121 IGEKAPCNLLFFGIEPQYLNLSAVNDGGKNVFLEDDPYKLSTFRVDSNHTRMYKIDYKFH 180
           I EK PCNLLFFGIE QYLNLSAVNDGG NVFLEDDPYKLSTF+V+SNHTRMYK+DYKFH
Sbjct: 121 IAEKTPCNLLFFGIESQYLNLSAVNDGGNNVFLEDDPYKLSTFKVNSNHTRMYKVDYKFH 180

Query: 181 AEKAYKLLKQARGNKSCAPDSGLRNSSKCKLALTDLPKEVYEVKWDVVVVDGPRGDSPHA 240
           AEKAYKLLK ARGNKSCAPDSGLRNSSKCKLALTDLP+EVYEVKWDVVVVDGPRGDSP A
Sbjct: 181 AEKAYKLLKHARGNKSCAPDSGLRNSSKCKLALTDLPREVYEVKWDVVVVDGPRGDSPQA 240

Query: 241 PGRMATIYSAAIMAREGNTTDVVVHDTDRMIERWYSWEFLCDENLISSKGKLWIFRIRNE 300
           PGRMATIYSAAI+AREGNTTDVVVHDTDRMIE+WYSWEFLCDENLISSKGKLWIFRIRNE
Sbjct: 241 PGRMATIYSAAIIAREGNTTDVVVHDTDRMIEKWYSWEFLCDENLISSKGKLWIFRIRNE 300

Query: 301 KNSSRFCPDKRI 312
           KNS+ FCPDK I
Sbjct: 301 KNSTSFCPDKVI 312

BLAST of Bhi08G001231 vs. ExPASy TrEMBL
Match: A0A5D3BHK3 (Protein IRX15-LIKE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004720 PE=4 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.5e-154
Identity = 281/312 (90.06%), Postives = 293/312 (93.91%), Query Frame = 0

Query: 1   MPPELVDCHSLVPSESLSPPSISPSQQSYPSVSFRNSRGSKSMSFTRNKILPVLVLILSS 60
           M P + DCHSLVPSE+LSPPSISPSQQS PSVSF NS+GSK+MSFTRNK+LPVLVLILSS
Sbjct: 1   MLPAVEDCHSLVPSEALSPPSISPSQQSIPSVSFTNSKGSKNMSFTRNKLLPVLVLILSS 60

Query: 61  LSILRLVRIALTTSYTSPLLAIALPPKLQRPLSGFSENH-RTSANEAAISETEFKLLSNL 120
           LSILRLVRIALTTS TSPLLAIALPPKLQRP SGFSE H RTSANEAAIS  EFKLLSNL
Sbjct: 61  LSILRLVRIALTTSNTSPLLAIALPPKLQRPKSGFSEKHNRTSANEAAISGKEFKLLSNL 120

Query: 121 IGEKAPCNLLFFGIEPQYLNLSAVNDGGKNVFLEDDPYKLSTFRVDSNHTRMYKIDYKFH 180
           I EK PCNLLFFGIE QYLNLSAVNDGG NVFLEDDPYKLSTF+V+SNHTRMYK+DYKFH
Sbjct: 121 IAEKTPCNLLFFGIESQYLNLSAVNDGGNNVFLEDDPYKLSTFKVNSNHTRMYKVDYKFH 180

Query: 181 AEKAYKLLKQARGNKSCAPDSGLRNSSKCKLALTDLPKEVYEVKWDVVVVDGPRGDSPHA 240
           AEKAYKLLK ARGNKSCAPDSGLRNSSKCKLALTDLP+EVYEVKWDVVVVDGPRGDSP A
Sbjct: 181 AEKAYKLLKHARGNKSCAPDSGLRNSSKCKLALTDLPREVYEVKWDVVVVDGPRGDSPQA 240

Query: 241 PGRMATIYSAAIMAREGNTTDVVVHDTDRMIERWYSWEFLCDENLISSKGKLWIFRIRNE 300
           PGRMATIYSAAI+AREGNTTDVVVHDTDRMIE+WYSWEFLCDENLISSKGKLWIFRIRNE
Sbjct: 241 PGRMATIYSAAIIAREGNTTDVVVHDTDRMIEKWYSWEFLCDENLISSKGKLWIFRIRNE 300

Query: 301 KNSSRFCPDKRI 312
           KNS+ FCPDK I
Sbjct: 301 KNSTSFCPDKVI 312

BLAST of Bhi08G001231 vs. ExPASy TrEMBL
Match: A0A0A0LRK1 (Polysacc_synt_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G050520 PE=4 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 4.9e-150
Identity = 274/310 (88.39%), Postives = 288/310 (92.90%), Query Frame = 0

Query: 7   DCHSLVPSESLSPPSISPSQQSYPSVSFRNSRGSKSMSFTRNKILPVLVLILSSLSILRL 66
           DCHSLVPSESLSPPSISPSQQS PSVSF NS+GSK+MSFTRNK+LPVLVLILSSLSILRL
Sbjct: 7   DCHSLVPSESLSPPSISPSQQSIPSVSFTNSKGSKNMSFTRNKLLPVLVLILSSLSILRL 66

Query: 67  VRIALTTSYTSPLLAIALPPKLQRPLSGFSENH-RTSANEAAISETEFKLLSNLIGEKAP 126
           VRIALTTS TSPLLAIALP KLQ P SGFSE   RTSANEAAIS  EFKLLSNLI EK P
Sbjct: 67  VRIALTTSNTSPLLAIALPSKLQHPKSGFSEKQSRTSANEAAISGKEFKLLSNLIAEKTP 126

Query: 127 CNLLFFGIEPQYLNLSAVNDGGKNVFLEDDPYKLSTFRVDSNHTRMYKIDYKFHAEKAYK 186
           CN+LFFGIE QYLNLSAVNDGG NVFLEDD YKLSTF+V+SNHTR YK+DYKFHAEKAYK
Sbjct: 127 CNVLFFGIESQYLNLSAVNDGGNNVFLEDDSYKLSTFKVNSNHTRTYKVDYKFHAEKAYK 186

Query: 187 LLKQARGNKSCAPDSGLRNSSKCKLALTDLPKEVYEVKWDVVVVDGPRGDSPHAPGRMAT 246
           LLK ARGNKSCAP+S LRNSSKCKLALTDLP+EVYEVKWDVVVVDGPRGDSP APGRMAT
Sbjct: 187 LLKHARGNKSCAPESALRNSSKCKLALTDLPREVYEVKWDVVVVDGPRGDSPQAPGRMAT 246

Query: 247 IYSAAIMAREGNTTDVVVHDTDRMIERWYSWEFLCDENLISSKGKLWIFRIRNEKNSSRF 306
           IYSAAI+AREGNTTDVVVHDTDRMIE+WYSWEFLCDENLISSKGKLWIFRIRNEKNS+ F
Sbjct: 247 IYSAAIIAREGNTTDVVVHDTDRMIEKWYSWEFLCDENLISSKGKLWIFRIRNEKNSTSF 306

Query: 307 CPDKRIVVQR 316
           CPDK IV+Q+
Sbjct: 307 CPDKGIVIQQ 316

BLAST of Bhi08G001231 vs. ExPASy TrEMBL
Match: A0A6J1C675 (glucuronoxylan 4-O-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC111008808 PE=4 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 3.5e-140
Identity = 255/316 (80.70%), Postives = 283/316 (89.56%), Query Frame = 0

Query: 1   MPPELVDCHSLVPSESL-SPPSISPSQQSYPSVSFRNSRGSKSMSFTRNKILPVLVLILS 60
           MPP+L DCH LV SESL SPPS+   QQ YPS+S +NSRGSKSM+FTR KI+PVLVL+LS
Sbjct: 1   MPPDLADCHPLVSSESLFSPPSVPSLQQPYPSLSLKNSRGSKSMNFTRKKIIPVLVLVLS 60

Query: 61  SLSILRLVRIALTTSYTSPLLAIALPPKLQRPLSGFSENH-RTSANEAAISETEFKLLSN 120
           SLSILRLVRIALTTS TS LLAIALPPKLQRP+ GFS N  R SANE AISE EFKLLSN
Sbjct: 61  SLSILRLVRIALTTSSTSSLLAIALPPKLQRPIFGFSINQSRASANEVAISEKEFKLLSN 120

Query: 121 LIGEKAPCNLLFFGIEPQYLNLSAVNDGGKNVFLEDDPYKLSTFRVDSNHTRMYKIDYKF 180
           LI +KAPCNLL FG+E QYLNLSA+NDGGKNVFLEDDPYKLSTF++ SNHTR+YK+DYK 
Sbjct: 121 LIAKKAPCNLLVFGLESQYLNLSAINDGGKNVFLEDDPYKLSTFKISSNHTRIYKVDYKH 180

Query: 181 HAEKAYKLLKQARGNKSCAPDSGLRNSSKCKLALTDLPKEVYEVKWDVVVVDGPRGDSPH 240
            AEKAYKLLK AR NK+CAP+SGL+N SKC+LALTDLP+EVYEVKWDVVVVDGP GD+P 
Sbjct: 181 PAEKAYKLLKHARQNKACAPNSGLKNLSKCQLALTDLPREVYEVKWDVVVVDGPIGDAPQ 240

Query: 241 APGRMATIYSAAIMAREGNTTDVVVHDTDRMIERWYSWEFLCDENLISSKGKLWIFRIRN 300
           APGRMATIYSAAI+AREGNTTDVV+HD DRMIE+WYSWEFLCDENLISS+GKLWIFRIRN
Sbjct: 241 APGRMATIYSAAIIAREGNTTDVVIHDIDRMIEKWYSWEFLCDENLISSRGKLWIFRIRN 300

Query: 301 EKNSSRFCPDKRIVVQ 315
           E NS+RFCPDK I++Q
Sbjct: 301 EINSTRFCPDKGIMIQ 316

BLAST of Bhi08G001231 vs. ExPASy TrEMBL
Match: A0A1S3BF86 (protein IRX15-LIKE OS=Cucumis melo OX=3656 GN=LOC103489024 PE=4 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 4.0e-136
Identity = 248/270 (91.85%), Postives = 256/270 (94.81%), Query Frame = 0

Query: 43  MSFTRNKILPVLVLILSSLSILRLVRIALTTSYTSPLLAIALPPKLQRPLSGFSENH-RT 102
           MSFTRNK+LPVLVLILSSLSILRLVRIALTTS TSPLLAIALPPKLQRP SGFSE H RT
Sbjct: 1   MSFTRNKLLPVLVLILSSLSILRLVRIALTTSNTSPLLAIALPPKLQRPKSGFSEKHNRT 60

Query: 103 SANEAAISETEFKLLSNLIGEKAPCNLLFFGIEPQYLNLSAVNDGGKNVFLEDDPYKLST 162
           SANEAAIS  EFKLLSNLI EK PCNLLFFGIE QYLNLSAVNDGG NVFLEDDPYKLST
Sbjct: 61  SANEAAISGKEFKLLSNLIAEKTPCNLLFFGIESQYLNLSAVNDGGNNVFLEDDPYKLST 120

Query: 163 FRVDSNHTRMYKIDYKFHAEKAYKLLKQARGNKSCAPDSGLRNSSKCKLALTDLPKEVYE 222
           F+V+SNHTRMYK+DYKFHAEKAYKLLK ARGNKSCAPDSGLRNSSKCKLALTDLP+EVYE
Sbjct: 121 FKVNSNHTRMYKVDYKFHAEKAYKLLKHARGNKSCAPDSGLRNSSKCKLALTDLPREVYE 180

Query: 223 VKWDVVVVDGPRGDSPHAPGRMATIYSAAIMAREGNTTDVVVHDTDRMIERWYSWEFLCD 282
           VKWDVVVVDGPRGDSP APGRMATIYSAAI+AREGNTTDVVVHDTDRMIE+WYSWEFLCD
Sbjct: 181 VKWDVVVVDGPRGDSPQAPGRMATIYSAAIIAREGNTTDVVVHDTDRMIEKWYSWEFLCD 240

Query: 283 ENLISSKGKLWIFRIRNEKNSSRFCPDKRI 312
           ENLISSKGKLWIFRIRNEKNS+ FCPDK I
Sbjct: 241 ENLISSKGKLWIFRIRNEKNSTSFCPDKVI 270

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G24910.18.2e-6543.12Protein of unknown function (DUF579) [more]
AT1G67330.11.1e-3734.97Protein of unknown function (DUF579) [more]
AT2G15440.11.4e-3536.07Protein of unknown function (DUF579) [more]
AT1G27930.12.2e-3338.62Protein of unknown function (DUF579) [more]
AT1G09610.13.2e-3234.42Protein of unknown function (DUF579) [more]
Match NameE-valueIdentityDescription
Q9SNE53.6e-3337.50Protein IRREGULAR XYLEM 15 OS=Arabidopsis thaliana OX=3702 GN=IRX15 PE=2 SV=1[more]
Q9C7F93.1e-3238.62Probable methyltransferase At1g27930 OS=Arabidopsis thaliana OX=3702 GN=At1g2793... [more]
Q6NMK14.4e-3134.42Glucuronoxylan 4-O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=GXM1 P... [more]
Q9FH925.8e-3135.50Protein IRX15-LIKE OS=Arabidopsis thaliana OX=3702 GN=IRX15-L PE=2 SV=1[more]
Q9LQ322.7e-2835.50Glucuronoxylan 4-O-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=GXM3 P... [more]
Match NameE-valueIdentityDescription
A0A5A7UNX62.5e-15490.06Protein IRX15-LIKE OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G00... [more]
A0A5D3BHK32.5e-15490.06Protein IRX15-LIKE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G0... [more]
A0A0A0LRK14.9e-15088.39Polysacc_synt_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G05... [more]
A0A6J1C6753.5e-14080.70glucuronoxylan 4-O-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A1S3BF864.0e-13691.85protein IRX15-LIKE OS=Cucumis melo OX=3656 GN=LOC103489024 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021148Polysaccharide biosynthesis domainPFAMPF04669Polysacc_synt_4coord: 112..293
e-value: 5.8E-56
score: 189.1
IPR006514IRX15/IRX15L/IGXMTIGRFAMTIGR01627TIGR01627coord: 96..306
e-value: 3.2E-73
score: 244.1
IPR006514IRX15/IRX15L/IGXMPANTHERPTHR31444OS11G0490100 PROTEINcoord: 13..309
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 14..40
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..40
NoneNo IPR availablePANTHERPTHR31444:SF34GLUCURONOXYLAN 4-O-METHYLTRANSFERASE-LIKE PROTEIN (DUF579)coord: 13..309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M001231Bhi08M001231mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0009834 plant-type secondary cell wall biogenesis
biological_process GO:0045492 xylan biosynthetic process
cellular_component GO:0000139 Golgi membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008168 methyltransferase activity