Bhi08G001149 (gene) Wax gourd (B227) v1

Overview


Name	Bhi08G001149
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	chr8: 41455285 .. 41456338 (+)
RNA-Seq Expression	Bhi08G001149
Synteny	Bhi08G001149

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTTCCAATGGCGTTTCTTCATCCATCTCCAAAAGGTACACAGAATTCTCAAACATTAAAACTTCACATCGAAATTAAACGAAAATCAACTCATTAATTTCTTCATCTCTGTTTTCTCTATTGCAGATTGGCCGGAAAAGTGGCTATAATAACCGGCGGAGCAAGTGGGATCGGCGAGTCGACGGTGAGGCTTTTTGTTGAGAATGGAGCCAAAGTGGTGATCGTTGATGTCCAGGACGACCTCGCCGCTGCTCTCTGCAAAGTGCTCGGCGACGATGTTTCCTATTTCCACTGCGACATTACCGACGAATCCGGCATCTCGAATGCCGTCGACTTTACTGTCCAAAAGTACAGAAAGCTTGACATCATGTTCAACAACGCTGGCATTCGTGGCAATGTAACAGCCACGACGATGACTACGGACATGAATGACTTCAAGAAAGTCTTCGATGTCAACGTGTTCGGTAGTTTCATGGGAGCTAAACACGCTGTCAGGTGAATTCAAAAGTTTATGTCCCGTGTTTATCAAATAATACCTTAAATCTTACGACTTTGTCACTGAAATCAAGAAATTTAAATCAATGGGTCATCGAAAATTTAATCATTTATTCATATTCTTTTCGTTAGAGTGATGGCTCCGGCGAAGACAGGGTGCATCCTGTTTACGTCAAGCATGGCATCGGTGATATGCAGTGGTAATGCGCCAGCGTATTCGGCATCGAAGCATGCAGTCGTAGGATTAATGAAGACGCTAGCCGTTGAATTGGGGCCTTACGGGATCAGAGTGAACGCGATATCGCCGTTCGCGACGGTGACACCGATGTTGCTTCCATCGAAGGATGTGGGGCAGAAGAAGGCAATGGAGGCCGTCATTTCTATGTCAGGGAACTTGAAAGGGGCAATAATGGAGGCTGAAGATGTAGCCAAAGCAGCTCTGTATTTAGCAAGTGATGAGTCCAAATATGTCAGTGGATTGAATCTTGTGGTTGATGGTGGCTTCAGTCTTACTAATCCTAGCTTTGAATTTAATACCAAAGCTTATGGACAATGA

mRNA sequence

ATGAGTTCCAATGGCGTTTCTTCATCCATCTCCAAAAGATTGGCCGGAAAAGTGGCTATAATAACCGGCGGAGCAAGTGGGATCGGCGAGTCGACGGTGAGGCTTTTTGTTGAGAATGGAGCCAAAGTGGTGATCGTTGATGTCCAGGACGACCTCGCCGCTGCTCTCTGCAAAGTGCTCGGCGACGATGTTTCCTATTTCCACTGCGACATTACCGACGAATCCGGCATCTCGAATGCCGTCGACTTTACTGTCCAAAAGTACAGAAAGCTTGACATCATGTTCAACAACGCTGGCATTCGTGGCAATGTAACAGCCACGACGATGACTACGGACATGAATGACTTCAAGAAAGTCTTCGATGTCAACGTGTTCGGTAGTTTCATGGGAGCTAAACACGCTGTCAGAGTGATGGCTCCGGCGAAGACAGGGTGCATCCTGTTTACGTCAAGCATGGCATCGGTGATATGCAGTGGTAATGCGCCAGCGTATTCGGCATCGAAGCATGCAGTCGTAGGATTAATGAAGACGCTAGCCGTTGAATTGGGGCCTTACGGGATCAGAGTGAACGCGATATCGCCGTTCGCGACGGTGACACCGATGTTGCTTCCATCGAAGGATGTGGGGCAGAAGAAGGCAATGGAGGCCGTCATTTCTATGTCAGGGAACTTGAAAGGGGCAATAATGGAGGCTGAAGATGTAGCCAAAGCAGCTCTGTATTTAGCAAGTGATGAGTCCAAATATGTCAGTGGATTGAATCTTGTGGTTGATGGTGGCTTCAGTCTTACTAATCCTAGCTTTGAATTTAATACCAAAGCTTATGGACAATGA

Coding sequence (CDS)

ATGAGTTCCAATGGCGTTTCTTCATCCATCTCCAAAAGATTGGCCGGAAAAGTGGCTATAATAACCGGCGGAGCAAGTGGGATCGGCGAGTCGACGGTGAGGCTTTTTGTTGAGAATGGAGCCAAAGTGGTGATCGTTGATGTCCAGGACGACCTCGCCGCTGCTCTCTGCAAAGTGCTCGGCGACGATGTTTCCTATTTCCACTGCGACATTACCGACGAATCCGGCATCTCGAATGCCGTCGACTTTACTGTCCAAAAGTACAGAAAGCTTGACATCATGTTCAACAACGCTGGCATTCGTGGCAATGTAACAGCCACGACGATGACTACGGACATGAATGACTTCAAGAAAGTCTTCGATGTCAACGTGTTCGGTAGTTTCATGGGAGCTAAACACGCTGTCAGAGTGATGGCTCCGGCGAAGACAGGGTGCATCCTGTTTACGTCAAGCATGGCATCGGTGATATGCAGTGGTAATGCGCCAGCGTATTCGGCATCGAAGCATGCAGTCGTAGGATTAATGAAGACGCTAGCCGTTGAATTGGGGCCTTACGGGATCAGAGTGAACGCGATATCGCCGTTCGCGACGGTGACACCGATGTTGCTTCCATCGAAGGATGTGGGGCAGAAGAAGGCAATGGAGGCCGTCATTTCTATGTCAGGGAACTTGAAAGGGGCAATAATGGAGGCTGAAGATGTAGCCAAAGCAGCTCTGTATTTAGCAAGTGATGAGTCCAAATATGTCAGTGGATTGAATCTTGTGGTTGATGGTGGCTTCAGTCTTACTAATCCTAGCTTTGAATTTAATACCAAAGCTTATGGACAATGA

Protein sequence

MSSNGVSSSISKRLAGKVAIITGGASGIGESTVRLFVENGAKVVIVDVQDDLAAALCKVLGDDVSYFHCDITDESGISNAVDFTVQKYRKLDIMFNNAGIRGNVTATTMTTDMNDFKKVFDVNVFGSFMGAKHAVRVMAPAKTGCILFTSSMASVICSGNAPAYSASKHAVVGLMKTLAVELGPYGIRVNAISPFATVTPMLLPSKDVGQKKAMEAVISMSGNLKGAIMEAEDVAKAALYLASDESKYVSGLNLVVDGGFSLTNPSFEFNTKAYGQ

Homology

[Close]

BLAST of Bhi08G001149 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 223.4 bits (568), Expect = 2.2e-58
Identity = 130/283 (45.94%), Postives = 180/283 (63.60%), Query Frame = 0


Query: 1   MSSNGVSSSI----SKRLAGKVAIITGGASGIGESTVRLFVENGAKVVIVDVQDDLAAAL 60
           MS+N  SSS     S+RL GKVA+ITGGA+GIGES VRLF ++GAKV IVD+QDDL   +
Sbjct: 1   MSTNTESSSYSSLPSQRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEV 60

Query: 61  CKVL-----GDDVSYFHCDITDESGISNAVDFTVQKYRKLDIMFNNAGIRGNVTATTMTT 120
           CK L      +   + H D+  E  ISNAVDF V+ +  LDI+ NNAG+ G         
Sbjct: 61  CKSLLRGESKETAFFIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNY 120

Query: 121 DMNDFKKVFDVNVFGSFMGAKHAVRVMAPAKTGCILFTSSMASVICSGNAPAYSASKHAV 180
            +++F+  FDVNV G+F+  KHA RVM P K G I+   S+  V+      +Y  SKHAV
Sbjct: 121 SLSEFEMTFDVNVKGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAV 180

Query: 181 VGLMKTLAVELGPYGIRVNAISPFATVTPMLLPSKDVGQKKAMEAVI------SMSGNLK 240
           +GL +++A ELG +GIRVN +SP+A  T + L      +++  +A +      + + NLK
Sbjct: 181 LGLTRSVAAELGQHGIRVNCVSPYAVATKLALAHLP-EEERTEDAFVGFRNFAAANANLK 240

Query: 241 GAIMEAEDVAKAALYLASDESKYVSGLNLVVDGGFSLTNPSFE 269
           G  +  +DVA A L+LASD+S+Y+SG NL++DGGF+ TN SF+
Sbjct: 241 GVELTVDDVANAVLFLASDDSRYISGDNLMIDGGFTCTNHSFK 282

[Close]

BLAST of Bhi08G001149 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 217.6 bits (553), Expect = 1.2e-56
Identity = 122/257 (47.47%), Postives = 174/257 (67.70%), Query Frame = 0


Query: 12  KRLAGKVAIITGGASGIGESTVRLFVENGAKVVIVDVQDDLAAALCKVLGDD-VSYFHCD 71
           KRL GK+ IITGGASGIG  +VRLF E+GA+VVIVDVQD+L   +   +G+D  SY+HCD
Sbjct: 4   KRLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGEDKASYYHCD 63

Query: 72  ITDESGISNAVDFTVQKYRKLDIMFNNAGIRGNVTATTMTTDMNDFKKVFDVNVFGSFMG 131
           +T+E+ + NAV FTV+KY KLD++F+NAG+      + +  ++N+  +   +N+ G+   
Sbjct: 64  VTNETEVENAVKFTVEKYGKLDVLFSNAGVI-EPFVSILDLNLNELDRTIAINLRGTAAF 123

Query: 132 AKHAVRVMA-PAKTGCILFTSSMASVICSGNAP-AYSASKHAVVGLMKTLAVELGPYGIR 191
            KHA R M      G I+ T+S+A+ I +G AP  Y+ SKH ++GL+K+ +  LG YGIR
Sbjct: 124 IKHAARAMVEKGIRGSIVCTTSVAAEI-AGTAPHGYTTSKHGLLGLIKSASGGLGKYGIR 183

Query: 192 VNAISPFATVTPMLLPSKDVGQKKAMEAVISMSGNLKGAIMEAEDVAKAALYLASDESKY 251
           VN ++PF   TP++     + +   +E   S S NLKG +++A  VA+AAL+LASDES Y
Sbjct: 184 VNGVAPFGVATPLVCNGFKM-EPNVVEQNTSASANLKGIVLKARHVAEAALFLASDESAY 243

Query: 252 VSGLNLVVDGGFSLTNP 266
           VSG NL VDGG+S+  P
Sbjct: 244 VSGQNLAVDGGYSVVKP 257

[Close]

BLAST of Bhi08G001149 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 216.5 bits (550), Expect = 2.7e-56
Identity = 126/264 (47.73%), Postives = 172/264 (65.15%), Query Frame = 0


Query: 5   GVSSSISKRLAGKVAIITGGASGIGESTVRLFVENGAKVVIVDVQDDLAAALCKVLGDDV 64
           G  +  SK+L GKVA+ITGGASG+G++T   F+ +GA+VVI D+  +      K LG + 
Sbjct: 32  GGCTCTSKKLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKELGSEA 91

Query: 65  SYFHCDITDESGISNAVDFTVQKYRKLDIMFNNAGIRGNVT-ATTMTTDMNDFKKVFDVN 124
            +  CD+T E+ I+ AV+ TV++Y KLD+M+NNAGI G +T A+    DM +F++V  +N
Sbjct: 92  EFVRCDVTVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTPASISQLDMTEFERVMRIN 151

Query: 125 VFGSFMGAKHAVRVMAPAKTGCILFTSSMASVICSGNAP-AYSASKHAVVGLMKTLAVEL 184
           VFG   G KHA + M PA++GCIL TSS+A V   G AP +Y+ SK    G++K+ A EL
Sbjct: 152 VFGVVSGIKHAAKFMIPARSGCILCTSSVAGV-TGGLAPHSYTISKFTTPGIVKSAASEL 211

Query: 185 GPYGIRVNAISPFATVTPMLLP-----SKDVGQKKAMEAVISMSGNLKGAIMEAEDVAKA 244
             +G+R+N ISP    TP+ L         V ++K  E V  M G LKGA  E  DVAKA
Sbjct: 212 CEHGVRINCISPGTVATPLTLSYLQKVFPKVSEEKLRETVKGM-GELKGAECEEADVAKA 271

Query: 245 ALYLASDESKYVSGLNLVVDGGFS 262
           ALYLAS++ KYV+G NLVVDGG +
Sbjct: 272 ALYLASNDGKYVTGHNLVVDGGMT 293

[Close]

BLAST of Bhi08G001149 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 213.0 bits (541), Expect = 3.0e-55
Identity = 121/266 (45.49%), Postives = 167/266 (62.78%), Query Frame = 0


Query: 1   MSSNGVSSSISKRLAGKVAIITGGASGIGESTVRLFVENGAKVVIVDVQDDLAAALCKVL 60
           +SS+  S S S++L GKVA+ITGGASGIG++T   F+ +GAKV+I D+Q  +     + L
Sbjct: 65  ISSSSSSHSTSRKLEGKVALITGGASGIGKATAGKFISHGAKVIIADIQPQIGRETEQEL 124

Query: 61  GDDVSYFHCDITDESGISNAVDFTVQKYRKLDIMFNNAGIRGNVTATTMTTDMNDFKKVF 120
           G   +YF CD+T ES I+NAVDF V  + KLDIM+NNAGI      + +  D+N F KV 
Sbjct: 125 GPSCAYFPCDVTKESDIANAVDFAVSLHTKLDIMYNNAGIPCKTPPSIVDLDLNVFDKVI 184

Query: 121 DVNVFGSFMGAKHAVRVMAPAKTGCILFTSSMASVICSGNAPAYSASKHAVVGLMKTLAV 180
           + NV G   G KHA RVM P  +G I+   S+  ++       YS SK AV+G++++ A 
Sbjct: 185 NTNVRGVMAGIKHAARVMIPRNSGSIICAGSVTGMMGGLAQHTYSVSKSAVIGIVRSTAS 244

Query: 181 ELGPYGIRVNAISPFATVTPMLLPS-----KDVGQKKAMEAVISMSGNLKGAIMEAEDVA 240
           EL  + IRVN ISPFA  T  ++         V   + ++ ++  +G L G + E  DVA
Sbjct: 245 ELCKHRIRVNCISPFAITTSFVMDEMRQIYPGVDDSRLIQ-IVQSTGVLNGEVCEPTDVA 304

Query: 241 KAALYLASDESKYVSGLNLVVDGGFS 262
            AA+YLASD+SKYV+G NLVVDGGF+
Sbjct: 305 NAAVYLASDDSKYVNGHNLVVDGGFT 329

[Close]

BLAST of Bhi08G001149 vs. TAIR 10
Match: AT2G47130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 211.5 bits (537), Expect = 8.8e-55
Identity = 120/255 (47.06%), Postives = 168/255 (65.88%), Query Frame = 0


Query: 13  RLAGKVAIITGGASGIGESTVRLFVENGAKVVIVDVQDDLAAALCKVLG-DDVSYFHCDI 72
           RL GK+AIITGGASGIG   VRLF ++GAKVVIVD Q++L   +   +G D  S++ CD+
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDFQEELGQNVAVSVGKDKASFYRCDV 64

Query: 73  TDESGISNAVDFTVQKYRKLDIMFNNAGIRGNVTATTMTTDMNDFKKVFDVNVFGSFMGA 132
           T+E  + NAV FTV+KY KLD++F+NAG+      + +  ++  F +   VNV G+    
Sbjct: 65  TNEKEVENAVKFTVEKYGKLDVLFSNAGVM-EQPGSFLDLNLEQFDRTMAVNVRGAAAFI 124

Query: 133 KHAVRVMAPAKT-GCILFTSSMASVICSGNAPAYSASKHAVVGLMKTLAVELGPYGIRVN 192
           KHA R M    T G I+ T+S+AS I      AY+ASKHA++GL+K+    LG YGIRVN
Sbjct: 125 KHAARAMVEKGTRGSIVCTTSVASEIGGPGPHAYTASKHALLGLVKSACGGLGKYGIRVN 184

Query: 193 AISPFATVTPMLLPSKDVGQKKAMEAVISMSGNLKGAIMEAEDVAKAALYLASDESKYVS 252
            ++P+A  T   + S+D    + +E   + +G LKG +++A  VA+AAL+LASD+S YVS
Sbjct: 185 GVAPYAVAT--AINSRDEETVRMVEEYSAATGILKGVVLKARHVAEAALFLASDDSAYVS 244

Query: 253 GLNLVVDGGFSLTNP 266
           G NL VDGG+S+  P
Sbjct: 245 GQNLAVDGGYSVVKP 256

[Close]

BLAST of Bhi08G001149 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.0e-67
Identity = 137/264 (51.89%), Postives = 187/264 (70.83%), Query Frame = 0


Query: 2   SSNGVSSSISKRLAGKVAIITGGASGIGESTVRLFVENGAKVVIVDVQDDLAAALCKVLG 61
           +++ V ++I++RL GKVA+ITGGASGIGE+T +LF ++GAKV I DVQD+L  ++ + +G
Sbjct: 3   ATSQVLTAIARRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIG 62

Query: 62  -DDVSYFHCDITDESGISNAVDFTVQKYRKLDIMFNNAGIRGNVTATTMTTDMNDFKKVF 121
             + +Y HCD+T+E G+ NAVD TV  Y KLDIMF+NAGI        +  +  DF++VF
Sbjct: 63  TSNSTYIHCDVTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVF 122

Query: 122 DVNVFGSFMGAKHAVRVMAPAKTGCILFTSSMASVICSGNAPAYSASKHAVVGLMKTLAV 181
            VNV G F+  KHA RVM PA++G I+ T+S++S +  G++ AY  SKHAV+GL + LAV
Sbjct: 123 SVNVTGVFLCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAV 182

Query: 182 ELGPYGIRVNAISPFATVTPMLLPSKDVGQKKAMEAVISMSGNLKGAIMEAEDVAKAALY 241
           ELG +GIRVN +SPF   T +      +  ++  E VI+ +GNLKG     EDVA AALY
Sbjct: 183 ELGQFGIRVNCLSPFGLPTALGKKFSGIKNEEEFENVINFAGNLKGPKFNVEDVANAALY 242

Query: 242 LASDESKYVSGLNLVVDGGFSLTN 265
           LASDE+KYVSG NL +DGGFS+ N
Sbjct: 243 LASDEAKYVSGHNLFIDGGFSVCN 266

[Close]

BLAST of Bhi08G001149 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 9.8e-67
Identity = 135/263 (51.33%), Postives = 179/263 (68.06%), Query Frame = 0


Query: 13  RLAGKVAIITGGASGIGESTVRLFVENGAKVVIVDVQDDLAAALCKVLGDD--VSYFHCD 72
           RL GKVA++TGGASGIGES  RLF+E+GAK+ IVDVQD+L   + + LG D    YFHCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 73  ITDESGISNAVDFTVQKYRKLDIMFNNAGIRGNVTATTMTTDMNDFKKVFDVNVFGSFMG 132
           +T E  +  AVDFT +KY  +DIM NNAGI G+        D N+FKKVFD+NV G F+G
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVFLG 121

Query: 133 AKHAVRVMAPAKTGCILFTSSMASVICSGNAPAYSASKHAVVGLMKTLAVELGPYGIRVN 192
            KHA R+M P   G I+  +S++SVI       Y+ +KHAVVGL K++A ELG +GIRVN
Sbjct: 122 MKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRVN 181

Query: 193 AISPFATVTPMLLP--SKDVGQKKAMEAVISM---SGNLKGAIMEAEDVAKAALYLASDE 252
            +SP+A  T + +P   +   Q+ A+   ++    + NLKG  +   DVA+A LYLA++E
Sbjct: 182 CVSPYAVPTRLSMPYLPESEMQEDALRGFLTFVRSNANLKGVDLMPNDVAEAVLYLATEE 241

Query: 253 SKYVSGLNLVVDGGFSLTNPSFE 269
           SKYVSGLNLV+DGGFS+ N + +
Sbjct: 242 SKYVSGLNLVIDGGFSIANHTLQ 264

[Close]

BLAST of Bhi08G001149 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 4.9e-66
Identity = 134/270 (49.63%), Postives = 178/270 (65.93%), Query Frame = 0


Query: 1   MSSNGVSSSISKRLAGKVAIITGGASGIGESTVRLFVENGAKVVIVDVQDDLAAALCKVL 60
           M S     S + RL  KVAIITGGA GIGE+T +LFV  GAKVVI D+ DD    +C  +
Sbjct: 1   MGSTSTPDSSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNI 60

Query: 61  G--DDVSYFHCDITDESGISNAVDFTVQKYRKLDIMFNNAGIRGNVTATTMTTDMNDFKK 120
           G  D +S+ HCD+T +  + N VD T+ K+ KLDIMF N G+      + +     DFK+
Sbjct: 61  GSPDVISFVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKR 120

Query: 121 VFDVNVFGSFMGAKHAVRVMAPAKTGCILFTSSMASVIC-SGNAPAYSASKHAVVGLMKT 180
           V D+NV+G+F+ AKHA RVM PAK G I+FT+S++S     G +  Y+A+KHAV+GL  +
Sbjct: 121 VMDINVYGAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTS 180

Query: 181 LAVELGPYGIRVNAISPFATVTPMLLPSKDVGQKKAMEAVISMSGNLKGAIMEAEDVAKA 240
           L  ELG YGIRVN +SP+   +P+L     V   + +E +   + NLKG ++ AEDVA A
Sbjct: 181 LCTELGEYGIRVNCVSPYIVASPLLTDVFGVDSSR-VEELAHQAANLKGTLLRAEDVADA 240

Query: 241 ALYLASDESKYVSGLNLVVDGGFSLTNPSF 268
             YLA DESKYVSGLNLV+DGG++ TNP+F
Sbjct: 241 VAYLAGDESKYVSGLNLVIDGGYTRTNPAF 269

[Close]

BLAST of Bhi08G001149 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.1e-65
Identity = 133/270 (49.26%), Postives = 179/270 (66.30%), Query Frame = 0


Query: 1   MSSNGVSSSISKRLAGKVAIITGGASGIGESTVRLFVENGAKVVIVDVQDDLAAALCKVL 60
           M S    +S + RL  KVAIITGGA GIGE+T +LFV  GAKVVI D+ DD    +C  +
Sbjct: 1   MGSTSTPASSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNI 60

Query: 61  G--DDVSYFHCDITDESGISNAVDFTVQKYRKLDIMFNNAGIRGNVTATTMTTDMNDFKK 120
           G  D +S+ HCD+T +  + N VD T+ K+ KLDIMF N G+      + +     DFK+
Sbjct: 61  GSPDVISFVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKR 120

Query: 121 VFDVNVFGSFMGAKHAVRVMAPAKTGCILFTSSMASVIC-SGNAPAYSASKHAVVGLMKT 180
           V D+NV+G+F+ AKHA RVM PAK G I+FT+S++S     G +  Y+A+KHAV+GL  +
Sbjct: 121 VMDINVYGAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTS 180

Query: 181 LAVELGPYGIRVNAISPFATVTPMLLPSKDVGQKKAMEAVISMSGNLKGAIMEAEDVAKA 240
           L  ELG +GIRVN +SP+   +P+L     V   + +E +   + NLKG ++ AEDVA A
Sbjct: 181 LCTELGQHGIRVNCVSPYVVASPLLTDVFGVDSSR-VEELAHQAANLKGILLRAEDVADA 240

Query: 241 ALYLASDESKYVSGLNLVVDGGFSLTNPSF 268
             YLA DESKYVSGLNLV+DGG++ TNP+F
Sbjct: 241 VAYLAGDESKYVSGLNLVIDGGYTRTNPAF 269

[Close]

BLAST of Bhi08G001149 vs. ExPASy Swiss-Prot
Match: H9BFQ0 (Tropinone reductase-like 1 OS=Erythroxylum coca OX=289672 PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 2.4e-65
Identity = 136/256 (53.12%), Postives = 174/256 (67.97%), Query Frame = 0


Query: 12  KRLAGKVAIITGGASGIGESTVRLFVENGAKVVIVDVQDDLAAALCKVLGDDVSYFHCDI 71
           KRL GKVAIITGGASGIG  T  LF ENGAKVVI D+QDDL  AL   LG    Y HCD+
Sbjct: 9   KRLEGKVAIITGGASGIGACTAELFHENGAKVVIADIQDDLGQALATKLGGKACYIHCDV 68

Query: 72  TDESGISNAVDFTVQKYRKLDIMFNNAGI--RGNVTATTMTTDMNDFKKVFDVNVFGSFM 131
           + E  + N VD TV KY +LDIMFNNAGI     +  + + ++ +D  ++  VN+ G+F+
Sbjct: 69  SKEDDVINLVDTTVAKYGRLDIMFNNAGIIEGQGLPVSVVESEKSDLDRLLSVNLGGAFL 128

Query: 132 GAKHAVRVMAPAKTGCILFTSSMASVICSGNAPAYSASKHAVVGLMKTLAVELGPYGIRV 191
           GAKHA RVM   + GCILFTSS+ + I   +  AY+ASK  V GL K L  ELG YGIRV
Sbjct: 129 GAKHATRVMVQQRKGCILFTSSLCTSIAGLSGHAYAASKSGVCGLAKNLTPELGKYGIRV 188

Query: 192 NAISPFATVTPMLLPSKDVGQKKAMEAVISMSGNLKGAIMEAEDVAKAALYLASDESKYV 251
           N ISP+  VT +   S+    ++ +EA++S  G L G  + A+ +AKAAL+LASDE+ YV
Sbjct: 189 NCISPYGLVTGISNISE--ANRELVEAMLSELGTLSGQTLRADGIAKAALFLASDEAYYV 248

Query: 252 SGLNLVVDGGFSLTNP 266
           SG+N+VVDGG+S+ NP
Sbjct: 249 SGINMVVDGGYSVVNP 262

[Close]

BLAST of Bhi08G001149 vs. ExPASy TrEMBL
Match: A0A5D3BK37 (Zerumbone synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002950 PE=4 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 3.6e-128
Identity = 242/280 (86.43%), Postives = 260/280 (92.86%), Query Frame = 0


Query: 1   MSSNGVSSSISKRLAGKVAIITGGASGIGESTVRLFVENGAKVVIVDVQDDLAAALCKVL 60
           MSSNGVSSSISKRLAGKVA+ITGGASGIGESTVRLFVENGAKVV+ DVQDDL A LCK L
Sbjct: 1   MSSNGVSSSISKRLAGKVALITGGASGIGESTVRLFVENGAKVVVADVQDDLGAVLCKEL 60

Query: 61  GD----DVSYFHCDITDESGISNAVDFTVQKYRKLDIMFNNAGIRGNVTATTMTTDMNDF 120
            D    +VSYFHCD+TDES ISNAVD+ V+KY KLDIMFNNAGIRG+V ATT+TTDMNDF
Sbjct: 61  DDGTGYNVSYFHCDVTDESDISNAVDYAVEKYGKLDIMFNNAGIRGDVGATTLTTDMNDF 120

Query: 121 KKVFDVNVFGSFMGAKHAVRVMAPAKTGCILFTSSMASVICSGNAPAYSASKHAVVGLMK 180
           KKVFDVNVFGSFMGAKHA RVMAPAKTGCILFTSSMASVICSGNAPAY+ASKHA++GLMK
Sbjct: 121 KKVFDVNVFGSFMGAKHAARVMAPAKTGCILFTSSMASVICSGNAPAYAASKHAIIGLMK 180

Query: 181 TLAVELGPYGIRVNAISPFATVTPMLLPSKDVGQKKAMEAVISMSGNLKGAIMEAEDVAK 240
           TLAVELGP GIRVNAISP+ATVTPML+PSK+V +KKAMEA IS+SGNL+GA+MEAEDVAK
Sbjct: 181 TLAVELGPKGIRVNAISPYATVTPMLVPSKNVEEKKAMEAFISLSGNLQGAVMEAEDVAK 240

Query: 241 AALYLASDESKYVSGLNLVVDGGFSLTNPSFEFNTKAYGQ 277
           AALYLASDESKYVSGLNLVVDGGFSLTNPSFE N K YGQ
Sbjct: 241 AALYLASDESKYVSGLNLVVDGGFSLTNPSFELNAKIYGQ 280

[Close]

BLAST of Bhi08G001149 vs. ExPASy TrEMBL
Match: A0A1S3AUF2 (zerumbone synthase OS=Cucumis melo OX=3656 GN=8D10 PE=4 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 3.6e-128
Identity = 242/280 (86.43%), Postives = 260/280 (92.86%), Query Frame = 0


Query: 1   MSSNGVSSSISKRLAGKVAIITGGASGIGESTVRLFVENGAKVVIVDVQDDLAAALCKVL 60
           MSSNGVSSSISKRLAGKVA+ITGGASGIGESTVRLFVENGAKVV+ DVQDDL A LCK L
Sbjct: 1   MSSNGVSSSISKRLAGKVALITGGASGIGESTVRLFVENGAKVVVADVQDDLGAVLCKEL 60

Query: 61  GD----DVSYFHCDITDESGISNAVDFTVQKYRKLDIMFNNAGIRGNVTATTMTTDMNDF 120
            D    +VSYFHCD+TDES ISNAVD+ V+KY KLDIMFNNAGIRG+V ATT+TTDMNDF
Sbjct: 61  DDGTGYNVSYFHCDVTDESDISNAVDYAVEKYGKLDIMFNNAGIRGDVGATTLTTDMNDF 120

Query: 121 KKVFDVNVFGSFMGAKHAVRVMAPAKTGCILFTSSMASVICSGNAPAYSASKHAVVGLMK 180
           KKVFDVNVFGSFMGAKHA RVMAPAKTGCILFTSSMASVICSGNAPAY+ASKHA++GLMK
Sbjct: 121 KKVFDVNVFGSFMGAKHAARVMAPAKTGCILFTSSMASVICSGNAPAYAASKHAIIGLMK 180

Query: 181 TLAVELGPYGIRVNAISPFATVTPMLLPSKDVGQKKAMEAVISMSGNLKGAIMEAEDVAK 240
           TLAVELGP GIRVNAISP+ATVTPML+PSK+V +KKAMEA IS+SGNL+GA+MEAEDVAK
Sbjct: 181 TLAVELGPKGIRVNAISPYATVTPMLVPSKNVEEKKAMEAFISLSGNLQGAVMEAEDVAK 240

Query: 241 AALYLASDESKYVSGLNLVVDGGFSLTNPSFEFNTKAYGQ 277
           AALYLASDESKYVSGLNLVVDGGFSLTNPSFE N K YGQ
Sbjct: 241 AALYLASDESKYVSGLNLVVDGGFSLTNPSFELNAKIYGQ 280

[Close]

BLAST of Bhi08G001149 vs. ExPASy TrEMBL
Match: A0A6J1K5R5 (zerumbone synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111490301 PE=4 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 1.6e-125
Identity = 237/280 (84.64%), Postives = 254/280 (90.71%), Query Frame = 0


Query: 1   MSSNGVSSSISKRLAGKVAIITGGASGIGESTVRLFVENGAKVVIVDVQDDLAAALCKVL 60
           MSSNGVS++ISKRLAGKVA+ITGGASGIG STVRLFVENGAKVVI DVQDDLAAALCK L
Sbjct: 1   MSSNGVSATISKRLAGKVALITGGASGIGASTVRLFVENGAKVVIADVQDDLAAALCKEL 60

Query: 61  GD----DVSYFHCDITDESGISNAVDFTVQKYRKLDIMFNNAGIRGNVTATTMTTDMNDF 120
            D    DVSYFHCD+TDES ISNAVDF V KY KLDIMFNNAGIRG+VTATTM TDMNDF
Sbjct: 61  DDATNHDVSYFHCDVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTMNTDMNDF 120

Query: 121 KKVFDVNVFGSFMGAKHAVRVMAPAKTGCILFTSSMASVICSGNAPAYSASKHAVVGLMK 180
           KKVFDVNVFG+FMGAKHA RVM PAK+GCILFTSSMASVICSGN PAYSASKHA+VGLMK
Sbjct: 121 KKVFDVNVFGTFMGAKHAARVMVPAKSGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180

Query: 181 TLAVELGPYGIRVNAISPFATVTPMLLPSKDVGQKKAMEAVISMSGNLKGAIMEAEDVAK 240
           TLAVELGP+GIRVNAISPFA VTPMLLPS D  QKKAME +I +SGNLKGA+M+ ED+AK
Sbjct: 181 TLAVELGPHGIRVNAISPFAAVTPMLLPSMDAEQKKAMETLIYLSGNLKGAMMDVEDIAK 240

Query: 241 AALYLASDESKYVSGLNLVVDGGFSLTNPSFEFNTKAYGQ 277
           AALYLASDESKYVSGLN+V+DGGFSLTNPSFE + KAY Q
Sbjct: 241 AALYLASDESKYVSGLNMVIDGGFSLTNPSFELSVKAYAQ 280

[Close]

BLAST of Bhi08G001149 vs. ExPASy TrEMBL
Match: A0A0A0LTA0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G044880 PE=4 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 1.1e-124
Identity = 235/279 (84.23%), Postives = 256/279 (91.76%), Query Frame = 0


Query: 1   MSSNGVSSSISKRLAGKVAIITGGASGIGESTVRLFVENGAKVVIVDVQDDLAAALCKVL 60
           MSSNGVSSSISKRLAGKVA+ITGGASGIGESTVRLFVENGAKVV+ DVQDDL A LCK L
Sbjct: 1   MSSNGVSSSISKRLAGKVALITGGASGIGESTVRLFVENGAKVVVADVQDDLGAVLCKEL 60

Query: 61  GD---DVSYFHCDITDESGISNAVDFTVQKYRKLDIMFNNAGIRGNVTATTMTTDMNDFK 120
            D   +VSYFHCD+TDES ISNAVD+ V+KY KLDIMFNNAGIRG+V +TT+T DMNDF+
Sbjct: 61  DDTGFNVSYFHCDVTDESDISNAVDYAVEKYGKLDIMFNNAGIRGDVGSTTLTADMNDFR 120

Query: 121 KVFDVNVFGSFMGAKHAVRVMAPAKTGCILFTSSMASVICSGNAPAYSASKHAVVGLMKT 180
           KVFDVNVFGSFMGAKHA RVMAPAKTGCILFTSSMASVICSGNAPAY+ASKHA++GLMKT
Sbjct: 121 KVFDVNVFGSFMGAKHAARVMAPAKTGCILFTSSMASVICSGNAPAYAASKHAIIGLMKT 180

Query: 181 LAVELGPYGIRVNAISPFATVTPMLLPSKDVGQKKAMEAVISMSGNLKGAIMEAEDVAKA 240
           LAVELG  GIRVNAISP+ATVTPML+PS++V +KKAMEA IS+SGNL+GA+MEAEDVAKA
Sbjct: 181 LAVELGSQGIRVNAISPYATVTPMLVPSRNVEEKKAMEAFISLSGNLQGAVMEAEDVAKA 240

Query: 241 ALYLASDESKYVSGLNLVVDGGFSLTNPSFEFNTKAYGQ 277
           ALYL SDESKYVSGLNLVVDGGFSLTNPSFE N K Y Q
Sbjct: 241 ALYLGSDESKYVSGLNLVVDGGFSLTNPSFELNAKIYAQ 279

[Close]

BLAST of Bhi08G001149 vs. ExPASy TrEMBL
Match: A0A6J1GSR4 (zerumbone synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111457191 PE=4 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 1.1e-124
Identity = 236/280 (84.29%), Postives = 254/280 (90.71%), Query Frame = 0


Query: 1   MSSNGVSSSISKRLAGKVAIITGGASGIGESTVRLFVENGAKVVIVDVQDDLAAALCKVL 60
           MSSNGVS++ISKRLAGKVA+ITGGASGIG STVRLFV+NGAKVVI DVQDDLAAALCK L
Sbjct: 1   MSSNGVSATISKRLAGKVALITGGASGIGASTVRLFVKNGAKVVIADVQDDLAAALCKEL 60

Query: 61  GD----DVSYFHCDITDESGISNAVDFTVQKYRKLDIMFNNAGIRGNVTATTMTTDMNDF 120
            D    DVSYFHCD+T+ES ISNAVDF V KY KLDIMFNNAGIRG+VTA TM TDMNDF
Sbjct: 61  NDATDHDVSYFHCDVTNESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTAMTMNTDMNDF 120

Query: 121 KKVFDVNVFGSFMGAKHAVRVMAPAKTGCILFTSSMASVICSGNAPAYSASKHAVVGLMK 180
           KKVFDVNVFG+FMGAKHA RVM PAK+GCILFTSSMASVICSGN PAYSASKHA+VGLMK
Sbjct: 121 KKVFDVNVFGTFMGAKHAARVMVPAKSGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180

Query: 181 TLAVELGPYGIRVNAISPFATVTPMLLPSKDVGQKKAMEAVISMSGNLKGAIMEAEDVAK 240
           TLAVELGP+GIRVNAISPFA VTPMLLPS DV QKKAME +I MSGNLKGA+M+ ED+AK
Sbjct: 181 TLAVELGPHGIRVNAISPFAAVTPMLLPSMDVEQKKAMETLIYMSGNLKGAMMDVEDIAK 240

Query: 241 AALYLASDESKYVSGLNLVVDGGFSLTNPSFEFNTKAYGQ 277
           AALYLASDESKYVSGLN+V+DGGFSLTNPSFE + KAY Q
Sbjct: 241 AALYLASDESKYVSGLNMVIDGGFSLTNPSFELSVKAYAQ 280

The following BLAST results are available for this feature:

BLAST of Bhi08G001149 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52340.1	2.2e-58	45.94	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47140.1	1.2e-56	47.47	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26770.1	2.7e-56	47.73	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G03140.1	3.0e-55	45.49	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47130.1	8.8e-55	47.06	NAD(P)-binding Rossmann-fold superfamily protein	[more]

BLAST of Bhi08G001149 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94KL7	2.0e-67	51.89	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
F1SWA0	9.8e-67	51.33	Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1	[more]
Q94KL8	4.9e-66	49.63	Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P...	[more]
A3F5F0	1.1e-65	49.26	Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ...	[more]
H9BFQ0	2.4e-65	53.13	Tropinone reductase-like 1 OS=Erythroxylum coca OX=289672 PE=2 SV=1	[more]

BLAST of Bhi08G001149 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BK37	3.6e-128	86.43	Zerumbone synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G0...	[more]
A0A1S3AUF2	3.6e-128	86.43	zerumbone synthase OS=Cucumis melo OX=3656 GN=8D10 PE=4 SV=1	[more]
A0A6J1K5R5	1.6e-125	84.64	zerumbone synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111490301 PE=4 SV=1	[more]
A0A0A0LTA0	1.1e-124	84.23	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G044880 PE=4 SV=1	[more]
A0A6J1GSR4	1.1e-124	84.29	zerumbone synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111457191 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 144..152 score: 37.09 coord: 89..100 score: 46.63 coord: 164..183 score: 42.43
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 164..183 score: 46.45 coord: 89..100 score: 50.68 coord: 185..202 score: 41.99 coord: 18..35 score: 47.63 coord: 224..244 score: 33.21 coord: 138..154 score: 28.87
None	No IPR available	GENE3D	3.40.50.720		coord: 3..274 e-value: 1.0E-79 score: 269.4
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 23..261 e-value: 1.8E-64 score: 217.5
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 14..263 e-value: 2.2E-19 score: 67.5
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 20..202 e-value: 3.7E-19 score: 66.8
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 8..269
None	No IPR available	PANTHER	PTHR43180:SF71	SECOISOLARICIRESINOL DEHYDROGENASE-LIKE	coord: 8..269
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 151..179
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 16..262

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi08M001149	Bhi08M001149	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity