Bhi08G001048 (gene) Wax gourd (B227) v1

Overview


Name	Bhi08G001048
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Non-specific lipid-transfer protein 2
Location	chr8: 38681874 .. 38682146 (-)
RNA-Seq Expression	Bhi08G001048
Synteny	Bhi08G001048

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGCATCACAGATTGCACTAAGTATGTTAGTGATGTTTATCATGGTTGAGATTTCATTGGCAGTGACATGCAGTCCAGTGGAACTGAGTTCCTGTGTTAGTGCACTCATATCTTCAGTTCCACCATCTAAGCTCTGTTGCTCTAAGATTAAGGAACAGAAACCCTGCCTCTGCAAGTACATGCAGAACCCCACTCTAAAGAAGTTTGTGGCCTCTCCCAATGCCAGGAAAGTTGCTACCACTTGTGGAACTCCATTTCCCAAGTGCTAG

mRNA sequence

ATGAAGGCATCACAGATTGCACTAAGTATGTTAGTGATGTTTATCATGGTTGAGATTTCATTGGCAGTGACATGCAGTCCAGTGGAACTGAGTTCCTGTGTTAGTGCACTCATATCTTCAGTTCCACCATCTAAGCTCTGTTGCTCTAAGATTAAGGAACAGAAACCCTGCCTCTGCAAGTACATGCAGAACCCCACTCTAAAGAAGTTTGTGGCCTCTCCCAATGCCAGGAAAGTTGCTACCACTTGTGGAACTCCATTTCCCAAGTGCTAG

Coding sequence (CDS)

ATGAAGGCATCACAGATTGCACTAAGTATGTTAGTGATGTTTATCATGGTTGAGATTTCATTGGCAGTGACATGCAGTCCAGTGGAACTGAGTTCCTGTGTTAGTGCACTCATATCTTCAGTTCCACCATCTAAGCTCTGTTGCTCTAAGATTAAGGAACAGAAACCCTGCCTCTGCAAGTACATGCAGAACCCCACTCTAAAGAAGTTTGTGGCCTCTCCCAATGCCAGGAAAGTTGCTACCACTTGTGGAACTCCATTTCCCAAGTGCTAG

Protein sequence

MKASQIALSMLVMFIMVEISLAVTCSPVELSSCVSALISSVPPSKLCCSKIKEQKPCLCKYMQNPTLKKFVASPNARKVATTCGTPFPKC

Homology

[Close]

BLAST of Bhi08G001048 vs. TAIR 10
Match: AT3G18280.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 1.1e-22
Identity = 47/87 (54.02%), Postives = 69/87 (79.31%), Query Frame = 0


Query: 4  SQIALSMLVMFIMVEISLAVTCSPVELSSCVSALISSVPPSKLCCSKIKEQKPCLCKYMQ 63
          S  AL+ +++ I+   + AVTCSP++LS C +A+ SS PPS LCC+K+KEQ+PCLC YM+
Sbjct: 10 SLFALAAVLLMILAPAAEAVTCSPMQLSPCATAITSSSPPSALCCAKLKEQRPCLCGYMR 69

Query: 64 NPTLKKFVASPNARKVATTCGTPFPKC 91
          NP+L++FV++PNARKV+ +C  P P+C
Sbjct: 70 NPSLRRFVSTPNARKVSKSCKLPIPRC 96

[Close]

BLAST of Bhi08G001048 vs. TAIR 10
Match: AT1G48750.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 102.1 bits (253), Expect = 2.5e-22
Identity = 46/90 (51.11%), Postives = 70/90 (77.78%), Query Frame = 0


Query: 1  MKASQIALSMLVMFIMVEISLAVTCSPVELSSCVSALISSVPPSKLCCSKIKEQKPCLCK 60
          M  S +AL+  ++ + V ++  VTCSP++L+SC +A+ SS PPS+ CC+K++EQ+PCLC 
Sbjct: 5  MWVSVLALAAAILLLTVPVAEGVTCSPMQLASCAAAMTSSSPPSEACCTKLREQQPCLCG 64

Query: 61 YMQNPTLKKFVASPNARKVATTCGTPFPKC 91
          YM+NPTL+++V+SPNARKV+ +C  P P C
Sbjct: 65 YMRNPTLRQYVSSPNARKVSNSCKIPSPSC 94

[Close]

BLAST of Bhi08G001048 vs. TAIR 10
Match: AT1G73780.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 80.5 bits (197), Expect = 7.7e-16
Identity = 36/83 (43.37%), Postives = 56/83 (67.47%), Query Frame = 0


Query: 10 MLVMFIMVEISLAVT--CSPVELSSCVSALISSVPPSKLCCSKIKEQKPCLCKYMQNPTL 69
          +L++ +  E  +A    C+P +LS C+  ++    PS LCCSK+KEQ+ C+C+Y++NP  
Sbjct: 16 LLILIVAQENRVAAVDPCNPAQLSPCLETIMKGSEPSDLCCSKVKEQQHCICQYLKNPNF 75

Query: 70 KKFVASPNARKVATTCGTPFPKC 91
          K F+ SPNA+ +AT C  P+PKC
Sbjct: 76 KSFLNSPNAKIIATDCHCPYPKC 98

[Close]

BLAST of Bhi08G001048 vs. TAIR 10
Match: AT5G38170.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 79.3 bits (194), Expect = 1.7e-15
Identity = 41/103 (39.81%), Postives = 62/103 (60.19%), Query Frame = 0


Query: 1   MKASQIALSMLVMFIMV--------EISLA-----VTCSPVELSSCVSALISSVPPSKLC 60
           MK + +   + V+ +MV        E+ L+     VTC  V+LSSC + +++ VPPS  C
Sbjct: 1   MKFTALVFIVFVVGVMVSPVSIRATEVKLSGGEADVTCDAVQLSSCATPMLTGVPPSTEC 60

Query: 61  CSKIKEQKPCLCKYMQNPTLKKFVASPNARKVATTCGTPFPKC 91
           C K+KEQ+PC C Y+++P   ++V S NA+K   TCG P+P C
Sbjct: 61  CGKLKEQQPCFCTYIKDPRYSQYVGSANAKKTLATCGVPYPTC 103

[Close]

BLAST of Bhi08G001048 vs. TAIR 10
Match: AT5G38160.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 78.2 bits (191), Expect = 3.8e-15
Identity = 34/68 (50.00%), Postives = 43/68 (63.24%), Query Frame = 0


Query: 23  VTCSPVELSSCVSALISSVPPSKLCCSKIKEQKPCLCKYMQNPTLKKFVASPNARKVATT 82
           VTC   +LSSCV+A+ +  PPS  CC K+KE + CLC Y+QNP    +V SPNARK    
Sbjct: 36  VTCDATQLSSCVTAVSTGAPPSTDCCGKLKEHETCLCTYIQNPLYSSYVTSPNARKTLAA 95

Query: 83  CGTPFPKC 91
           C   +P C
Sbjct: 96  CDVAYPTC 103

[Close]

BLAST of Bhi08G001048 vs. ExPASy Swiss-Prot
Match: P82353 (Non-specific lipid-transfer protein 2 OS=Prunus armeniaca OX=36596 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.8e-17
Identity = 37/68 (54.41%), Postives = 52/68 (76.47%), Query Frame = 0


Query: 23 VTCSPVELSSCVSALISSVPPSKLCCSKIKEQKPCLCKYMQNPTLKKFVASPNARKVATT 82
          VTCSPV+LS C+  + S  P    CC K++EQ+PCLC Y++NP+L+++V SPNARK+A+ 
Sbjct: 1  VTCSPVQLSPCLGPINSGAPSPTTCCQKLREQRPCLCGYLKNPSLRQYVNSPNARKLASN 60

Query: 83 CGTPFPKC 91
          CG P P+C
Sbjct: 61 CGVPVPQC 68

[Close]

BLAST of Bhi08G001048 vs. ExPASy Swiss-Prot
Match: Q43681 (Probable non-specific lipid-transfer protein AKCS9 OS=Vigna unguiculata OX=3917 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 3.0e-17
Identity = 43/93 (46.24%), Postives = 63/93 (67.74%), Query Frame = 0


Query: 1  MKASQIALSMLVMFIMVE---ISLAVTCSPVELSSCVSALISSVPPSKLCCSKIKEQKPC 60
          MK S +   ++V   +++   ++ AVTC+P ELSSCV A+     PS  CCSK+K Q+PC
Sbjct: 7  MKMSVVCAVVVVALFLIDVGPVAEAVTCNPTELSSCVPAITGGSKPSSTCCSKLKVQEPC 66

Query: 61 LCKYMQNPTLKKFVASPNARKVATTCGTPFPKC 91
          LC Y++NP+LK++V SP A+KV + CG  +P C
Sbjct: 67 LCNYIKNPSLKQYVNSPGAKKVLSNCGVTYPNC 99

[Close]

BLAST of Bhi08G001048 vs. ExPASy Swiss-Prot
Match: P86809 (Non-specific lipid-transfer protein 2 OS=Apium graveolens var. rapaceum OX=278110 PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 2.2e-15
Identity = 37/67 (55.22%), Postives = 50/67 (74.63%), Query Frame = 0


Query: 24 TCSPVELSSCVSALISSVPPSKLCCSKIKEQKPCLCKYMQNPTLKKFVASPNARKVATTC 83
          TCS V+LS C++A+  + PPS  CC+K+KEQKPCLC Y+++P LK +V SP ARK A++C
Sbjct: 2  TCSAVQLSPCLAAITKNTPPSAACCNKLKEQKPCLCGYLKDPNLKNYVNSPGARKTASSC 61

Query: 84 GTPFPKC 91
          G    KC
Sbjct: 62 GVAL-KC 67

[Close]

BLAST of Bhi08G001048 vs. ExPASy Swiss-Prot
Match: P82901 (Non-specific lipid-transfer protein 2P OS=Triticum aestivum OX=4565 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 2.3e-09
Identity = 25/66 (37.88%), Postives = 40/66 (60.61%), Query Frame = 0


Query: 25 CSPVELSSCVSALISSVPPSKLCCSKIKEQKPCLCKYMQNPTLKKFVASPNARKVATTCG 84
          C   +L+ C SA++S   PS  CC  ++ Q+PC C+Y ++PT  +++ SP+AR    +CG
Sbjct: 2  CQASQLAVCASAILSGAKPSGECCGNLRAQQPCFCQYAKDPTYGQYIRSPHARDTLQSCG 61

Query: 85 TPFPKC 91
             P C
Sbjct: 62 LAVPHC 67

[Close]

BLAST of Bhi08G001048 vs. ExPASy Swiss-Prot
Match: P82900 (Non-specific lipid-transfer protein 2G OS=Triticum aestivum OX=4565 PE=1 SV=2)

HSP 1 Score: 58.2 bits (139), Expect = 5.7e-08
Identity = 29/92 (31.52%), Postives = 48/92 (52.17%), Query Frame = 0


Query: 1  MKASQIALSMLVMFIMVEI--SLAVTCSPVELSSCVSALISSVPPSKLCCSKIKEQKPCL 60
          MK   +   ML + ++          C   +L+ C SA++S   PS  CC  ++ Q+ C 
Sbjct: 5  MKKQVVTALMLALVVLAAAPGGARAACQASQLAVCASAILSGAKPSGECCGNLRAQQGCF 64

Query: 61 CKYMQNPTLKKFVASPNARKVATTCGTPFPKC 91
          C+Y ++PT  +++ SP+AR   T+CG   P C
Sbjct: 65 CQYAKDPTYGQYIRSPHARDTLTSCGLAVPHC 96

[Close]

BLAST of Bhi08G001048 vs. ExPASy TrEMBL
Match: A0A0A0LVT5 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042560 PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 3.0e-36
Identity = 80/90 (88.89%), Postives = 87/90 (96.67%), Query Frame = 0


Query: 1  MKASQIALSMLVMFIMVEISLAVTCSPVELSSCVSALISSVPPSKLCCSKIKEQKPCLCK 60
          MKASQIALS++VMF+ VEI++AVTCSPV+LSSCVSA+ SSVPPSKLCCSKIKEQKPCLCK
Sbjct: 1  MKASQIALSIIVMFMTVEITMAVTCSPVQLSSCVSAITSSVPPSKLCCSKIKEQKPCLCK 60

Query: 61 YMQNPTLKKFVASPNARKVATTCGTPFPKC 91
          YMQNPTLKKFVASPNARKVA TCGTPFPKC
Sbjct: 61 YMQNPTLKKFVASPNARKVANTCGTPFPKC 90

[Close]

BLAST of Bhi08G001048 vs. ExPASy TrEMBL
Match: A0A5D3BJV8 (Non-specific lipid-transfer protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002150 PE=4 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 4.0e-36
Identity = 79/90 (87.78%), Postives = 88/90 (97.78%), Query Frame = 0


Query: 1  MKASQIALSMLVMFIMVEISLAVTCSPVELSSCVSALISSVPPSKLCCSKIKEQKPCLCK 60
          MKASQIALS++VMF+MVEIS+AVTCSPV+LSSCVSA+ SSVPPS LCCSKIKEQ+PCLCK
Sbjct: 1  MKASQIALSIIVMFMMVEISMAVTCSPVQLSSCVSAITSSVPPSSLCCSKIKEQRPCLCK 60

Query: 61 YMQNPTLKKFVASPNARKVATTCGTPFPKC 91
          YMQNPTLKKFVASPNA+KVA+TCGTPFPKC
Sbjct: 61 YMQNPTLKKFVASPNAKKVASTCGTPFPKC 90

[Close]

BLAST of Bhi08G001048 vs. ExPASy TrEMBL
Match: A0A6J1ECM2 (non-specific lipid-transfer protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111431330 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 8.8e-36
Identity = 78/90 (86.67%), Postives = 87/90 (96.67%), Query Frame = 0


Query: 1  MKASQIALSMLVMFIMVEISLAVTCSPVELSSCVSALISSVPPSKLCCSKIKEQKPCLCK 60
          MKASQIA+S+LVMF+M E+S+AVTCSP++LSSCVSA+ SSVPPSKLCCSKIKEQKPCLCK
Sbjct: 1  MKASQIAVSILVMFMMAEVSVAVTCSPLQLSSCVSAITSSVPPSKLCCSKIKEQKPCLCK 60

Query: 61 YMQNPTLKKFVASPNARKVATTCGTPFPKC 91
          YMQNPTL+KFVASPNARKVA TCGTPFPKC
Sbjct: 61 YMQNPTLRKFVASPNARKVANTCGTPFPKC 90

[Close]

BLAST of Bhi08G001048 vs. ExPASy TrEMBL
Match: A0A6J1KNT4 (probable non-specific lipid-transfer protein AKCS9 OS=Cucurbita maxima OX=3661 GN=LOC111495166 PE=4 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.0e-35
Identity = 77/90 (85.56%), Postives = 87/90 (96.67%), Query Frame = 0


Query: 1   MKASQIALSMLVMFIMVEISLAVTCSPVELSSCVSALISSVPPSKLCCSKIKEQKPCLCK 60
           MKASQIA+++LVMF+M E+S+AVTCSP++LSSCVSA+ SSVPPSKLCCSKIKEQKPCLCK
Sbjct: 51  MKASQIAVTILVMFMMAEVSVAVTCSPLQLSSCVSAITSSVPPSKLCCSKIKEQKPCLCK 110

Query: 61  YMQNPTLKKFVASPNARKVATTCGTPFPKC 91
           YMQNPTL+KFVASPNARKVA TCGTPFPKC
Sbjct: 111 YMQNPTLRKFVASPNARKVANTCGTPFPKC 140

[Close]

BLAST of Bhi08G001048 vs. ExPASy TrEMBL
Match: V7C3M6 (AAI domain-containing protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_004G154200g PE=4 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 6.1e-29
Identity = 68/92 (73.91%), Postives = 81/92 (88.04%), Query Frame = 0


Query: 1  MKASQIALSMLVMFIM--VEISLAVTCSPVELSSCVSALISSVPPSKLCCSKIKEQKPCL 60
          MKA+ IAL  LV+ ++  V++S+AVTCSPV+LSSCVSA+ SS PPS LCCSKIKEQKPCL
Sbjct: 1  MKAAHIALCTLVVLLVAEVQVSMAVTCSPVQLSSCVSAITSSTPPSNLCCSKIKEQKPCL 60

Query: 61 CKYMQNPTLKKFVASPNARKVATTCGTPFPKC 91
          CKY++NP LKKFV SPNARKVA+TCGTPFP+C
Sbjct: 61 CKYLKNPNLKKFVDSPNARKVASTCGTPFPRC 92

The following BLAST results are available for this feature:

BLAST of Bhi08G001048 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G18280.1	1.1e-22	54.02	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G48750.1	2.5e-22	51.11	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G73780.1	7.7e-16	43.37	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT5G38170.1	1.7e-15	39.81	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT5G38160.1	3.8e-15	50.00	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]

BLAST of Bhi08G001048 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P82353	1.8e-17	54.41	Non-specific lipid-transfer protein 2 OS=Prunus armeniaca OX=36596 PE=1 SV=1	[more]
Q43681	3.0e-17	46.24	Probable non-specific lipid-transfer protein AKCS9 OS=Vigna unguiculata OX=3917 ...	[more]
P86809	2.2e-15	55.22	Non-specific lipid-transfer protein 2 OS=Apium graveolens var. rapaceum OX=27811...	[more]
P82901	2.3e-09	37.88	Non-specific lipid-transfer protein 2P OS=Triticum aestivum OX=4565 PE=1 SV=1	[more]
P82900	5.7e-08	31.52	Non-specific lipid-transfer protein 2G OS=Triticum aestivum OX=4565 PE=1 SV=2	[more]

BLAST of Bhi08G001048 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVT5	3.0e-36	88.89	AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042560 PE=4 SV...	[more]
A0A5D3BJV8	4.0e-36	87.78	Non-specific lipid-transfer protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A6J1ECM2	8.8e-36	86.67	non-specific lipid-transfer protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1KNT4	2.0e-35	85.56	probable non-specific lipid-transfer protein AKCS9 OS=Cucurbita maxima OX=3661 G...	[more]
V7C3M6	6.1e-29	73.91	AAI domain-containing protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_004G154200g...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF14368	LTP_2	coord: 22..86 e-value: 1.0E-6 score: 28.8
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 23..90 e-value: 1.7E-22 score: 81.4
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 24..90
None	No IPR available	PANTHER	PTHR33214:SF30	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN	coord: 2..90
IPR033872	Non-specific lipid-transfer protein type 2	PANTHER	PTHR33214	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN	coord: 2..90
IPR033872	Non-specific lipid-transfer protein type 2	CDD	cd01959	nsLTP2	coord: 25..90 e-value: 7.83771E-26 score: 88.9098

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi08M001048	Bhi08M001048	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006869	lipid transport