Bhi08G000968 (gene) Wax gourd (B227) v1

Overview


Name	Bhi08G000968
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	BPL/LPL catalytic domain-containing protein
Location	chr8: 36275640 .. 36280950 (-)
RNA-Seq Expression	Bhi08G000968
Synteny	Bhi08G000968

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGCACATATATTATATTAATTAACAAAATAAGTCAAATGGCAAAATAAAAGGGGTATTGGCATGCGTGAGGTTGAATCCCCCAACCCCAAATATTGCATTAGACTGCAACATTAGATCAATATTTAGTGAAATTTAAGTTTAGTTCATTCAAGACAGATCTGTCGTGTTTCATCTCCGGCCAACGCAATTAGGGCACGAAAATCTGGCGATTTTGATCATCATTTTTGTAGAATCGGTTCTATCGTTGCATTGATTTTTTGCTCTTCGGTCCTATTTCTGCAGCGGAAGATCATTCTCAGTCTCCGCCCTCTGCATTAGATTGGTTCTTTAAGTAGTAGTTTTCTGCAAAGCCACTCACTTGCCCAGATTATTCTTCGTTCACGATCATCGAAAATTGATTTTTTGTTGAATAATCCCAGAAATTTATATCTCTCTCGGCCAAGAGATTGGGTCTGGTTTTTGAGAATTTTTGGAATGGATCTGTTTTTCCTGATTGCTGGGAGTATTTTTCTAGTAAGATTTTTTTTTTTTAATTCCTCAATCCTTCCTTCGCCTTGTAGTTCTTATTGGCTGTTTTTTTTTCTTTTATTGAGTTATGCAAGCAAGTCTCATTATAGTATATACTCATTTTTTCTTTTTTGGATCTGTTGATGAACACTGTATTTCTCCATTGTCATTGATTCAGAACCAATGTGCAGGTTTTGGTTACTTTGACTTTCTTGTGTTACAAAGTTCTTTATGTACCTCGGTATATTACATTATCAATTGCTTTTGTCTACTGCATTATGTTATTCCATCTGTGCCCAGCAGCATCTCGGAGACGGCTAATATTTATCTCCATTCTAACTGTTGCCCCGATGTTAGAATTGCATTTATTTATTTATTTTGTTTTTAAATCTGAGGCTAAGGTGATACAATGATATAGTGGCTTAATTTGGAATGGACATGCTTCTTTGTGGTGCAGGGAGTTATGGCAGCTTAATTGAAGTCGACTTCTTTTTTGGAAGTAGGAGAAGAAAAGAAATCTCATTATCAAAATTAGGGCTTCCCAATGACGATGTATCAAACTAGAAACATTGGGTATCCTTTGATCAATCTTGTGAGATTGAAAGGTTTTCCTATTCTGCAGCAATTACAATTGGAAGAGCGCTTACTTCGAAACTCATCAGATAATTGGTGTATCATAAATGATGGAACAGATAATCCAACTGTTGTGGTGGGCATTTCTGGGTACATACCATAACATGTAGATGTAGTTGTACTTCGAATTCTGCATTTGTCTTATGTTGCTATCTATTGGAAGACTTAATTTTTCTTTTTCTCTTGATTATTTGGGGTTTATGGTGCTCATAATGATATAGTGACCATCAACCTTATATGTCACAGTTTGATGATACATAAATGTTATATTCAAATACCTGTACAAATATTTCCAATGAACCATTGAATCGTGCAATAAAGGGAAACTGTTATGAAATGATTGAATCTGTCACTACAAGTCTACAAGTTGGAATGAGGTTTACAATTTGACTGAGTTTCATTGCTCATTTTGGTGTTCCCTGTCTAATTCTTTTTGTCAATTCACCTCTTTATGGCTCACAAAAGTTGGGTGTCATTTTGTTGTAACTTTTCACCAAATCATTAAAAGGTTTGTTTCTATAAACAAAAAGCTCTCTTTATGAACTGTTCCTGGATGTAGAAGAGTTGCTATTTCAATTCCTTCTGGCTGCATTGATCAGTTAGAGAGAAACTTATTGAACTTTAATTTACATGAAGTCTTCTTTTGATATGGATTAGTAACAACTTGATGATAGTAATAATGACTAGCAATTTGGATGGTTGTGTTTAGCCTTGAGTAGTGCCTAATTCTGGGTCATCTGATCTTTTCTTTTGTTTTTTCTTATAGTTTATTCCTTCTGAAGATTTTGAATTCTGCACAGGAAACCTGATGAGCTCCTTGACATCAAATCTGTGTTAGGAGATCAAATTCCAGTCATCAGAAGGTTCACAGGGGGTGGAACTGTTATTGTTGATCACAACACAGTATTTGTCACCTTCATATGTAATAAAGATGCTGTTTCCGGACTAAAACCATTTCCGCAACCTATCATGTCCTGGAGTAGTCAACTATATAATAAAGTATTTCAAGGAACTGACTTTTATCTTCGGGAAAATGGTAGGTAGATTGTGCTGTTTAGAATAGACGCCATTTCAACTTACTCATTTAACTTTGTGTGCATTCAGTTATGTATAAGATTAAACGCTTGGACATTGACTTTTTGTTTTCTTTGTACTAAGATTATGTCTTTGGTAACCGCAAGTTTGGTGGCAATGCTCAGTCCATTACTAAAAGCCGGTGGATCCACCATACATCTTTTCTGTGGGACTATGAGGTTAGGAATATGACATATCTGAAACATCCAAAACGGACTCCTGATTACCGTGAGGTATGGTGTACTGTTGCATTTTTTTCTTTCTTTCCAAACTGATTTCATGTTTCATCTGCTTATTTTTCCATGAGCAAGCGCAAGCCTTAGTACTGCAATTCAAAATGCAAGTATATTTAACAGCTTCAAGACAAATAAATTATTCTTTTTTTTTTAATTATTCTTACTAATAACTAATCATATTGTCTCTCTGTTAGTTCGCCTTTGTATAATTGAGAAGTTCCTTGACAATTGCCATATCTCTGGCTACTGTCTCCTAAGCTCATCTTACGGCTGCAATTAGGAATATCCATGTGTTGGGTTTAGTGAAAAACATTTGGGGACCAATCTAAATGTTGAGTCAGCAGATTTTCCCCCACCAAAATTAGACCTAAGTTAGGTGATGGCATGCGTGATGCCCAGCCCAACCTGAACCATATAATAGTTTGATTGGGTTGGTTCAAATTATTGTGGCTATATATTTCAAGATTGTGTGAAAGAATTGTGAAAATGATAGCACAGTGGCTAATTAGCCAGTCACACATCGGCTAAGTAGCTGTATGTTCTGTGTGAATGGTCAGTAAGCTCACCTAGAGCAATGTCGTTTGACAGCCATTGTAGTGGAGGTAGATCTTGTGGCTTCACGCCAAGGCTGTCAATGTAGATATCTAAAAAACCATGTTCAAACTCGAAATAGTGAAAATGATAGCATTCAATGGACCTATTTTCACACCCTGAACCTGAATTAAATGTAATATATAGTGCAACCTGCTATCTTAACTTATGAAAAACAACGGTAATATTCTTGAGACTAGAACCAATTATTTTATATAAGTTGTAAAGAGAATAAAGAATATAATGGAGTTATTTGGTAGCCACAATGAATTGGTCTATTGAGTAATAACTAGTAACGACCAAGATAAATAGTAAAGGGTTTATAGAGAATGAGTTCAAATCATGGTGACCACCTAAGGGTTAGGTTATCATCATGTGAAATTAGTCGAGGTATGTGGAAACTGGTTTGGACACACAAAAAAAAGGTTATTTATGCAACCTAGCTCCATGATCTAGAAATTCGAAGTCTTTCTACAATTTCTTAGAGATCTCAGAAGAAGTTTGTAACCTAACCCTAATATTTTTTGTTTGAGTTGTTTGGGTCCTTTTGGACCAACATCCTTGTTACAATAAATTGGAGAAAGTGGAAGACTAAAACACATGTTGACATGGCATTCAGTTACAATATGATAATTGACACGGTTAATGTTGTCAGATGCTGCTTAATGTTGGGTTGTCTGAATTTTATTTGTACTAAATGCAAAACACCAAATTCTATTTGCAATAAAATGCAAAATTCGAGCTTACTGACAAACATTTATATGTTTCAGGCAAGGAGCCATTTGGATTTTTTATGCAGCTTGAAGGACTACATGCCAAGACAGGTATTTATTGACAAAACAATAGAAGCAGTTGAAACTGAGTTTGCTACGAGTTTAAAGAAATTAAAAGAATGTGAATATTCTTTGACTGGAAACTACAGCCCCACAACAAGGATGCTGACAGAGCAAGAATTGGAAACAGCTGCAGGTTTATCACAAGATAATAAGCTTGACAAAGTCTTGGACATTGCATTATAACATCTTGGTATATGATTATTTCACCCCAAATATTAAATTTTACATGCCCCTCATTTATATTAATCTTGGAATCATTTCAAGTTTTAGCTTTATTTATTGCTTTGCAACCCCTTTCTCGCTTGGAATATTCTTGTTCTTGCCTGTTAACATTGAATTAGCATGGCCAGTCTAATTCGAGTCACAATGCACTGTTAGCCAACCCTCAAGGTATATTATATTTGTAGCTTCTTGAATTTCTAGATCGTAGAACGAGATTGCTTCAATTTGTGGTATATCCTAGACTGTCTTACAGATGCTAGGACATGTGTACGTCGTTATCAAACCAATTTTTGGGACTAGTAGTTAGTTTATCTATTAGAGCCTTCGCGGTGCAATTGAGTGGTGATAACTTGTGAAATTGGAAGGTTTGAGTATTGGTTCAATGTGTGAAGATGGATATCCTAGCTATCCTAGCTAGGGAGGGTGTATTTTTTCATAAGATGGAATCATGGAAGATGCAAGAACAATATTGTAGTTAACTTTCCGTTGTACAGGCGCAACCTTCTAGTTTTTGACGATTGCATCTCTCCACATCTCTCCCAACTCCCATCTTATTCTGTGGCCGATGAACACGGTGGATTCCTTTGATCGTCAAGTTGGGCAAGCCCTGCCTCAATTGTCTAGTCCTCTCGATCAATGATGAGGCCTCTTGTTAGTCGGCACCTCCTCACGGATCGTTTAAGGTGAACTATGGTGCGGCCTTCTTGAATGGTGATGTGGGTATTGGAGTCCTTGTTCCCGACTCAAATGGTGATGTTATGCTTGGTATGGAAAGTCACACTTCTATTGCAGGATCTGTTGTGCTCGTAGAGGCTATTGTCATATTTAATGGTTTGTTTAGAGCTCTTGAGGTTGGTATTTCCCACTTTGGGCTAAAATTGATTTCCTAATTCTATGGAATCTTTTGGTTGAGAAAACCATATTTGCGAATGAGTTTCAGATTTTTGTGGTTTCTATTCGATCTCCATCACAGTGGCTCTATCCATGACTTTCCTTTGGCCAACTGCAAGAATCATTATGTTGCTCATGAGTTAGCTATCAATACGCGAAGATTTGAATATTCAACAATTTAGCTTGTTAGTCC

mRNA sequence

TTTGCACATATATTATATTAATTAACAAAATAAGTCAAATGGCAAAATAAAAGGGGTATTGGCATGCGTGAGGTTGAATCCCCCAACCCCAAATATTGCATTAGACTGCAACATTAGATCAATATTTAGTGAAATTTAAGTTTAGTTCATTCAAGACAGATCTGTCGTGTTTCATCTCCGGCCAACGCAATTAGGGCACGAAAATCTGGCGATTTTGATCATCATTTTTGTAGAATCGGTTCTATCGTTGCATTGATTTTTTGCTCTTCGGTCCTATTTCTGCAGCGGAAGATCATTCTCAGTCTCCGCCCTCTGCATTAGATTGGTTCTTTAAGTAGTAGTTTTCTGCAAAGCCACTCACTTGCCCAGATTATTCTTCGTTCACGATCATCGAAAATTGATTTTTTGTTGAATAATCCCAGAAATTTATATCTCTCTCGGCCAAGAGATTGGGTCTGGTTTTTGAGAATTTTTGGAATGGATCTGTTTTTCCTGATTGCTGGGAGTATTTTTCTAGGAGTTATGGCAGCTTAATTGAAGTCGACTTCTTTTTTGGAAGTAGGAGAAGAAAAGAAATCTCATTATCAAAATTAGGGCTTCCCAATGACGATGTATCAAACTAGAAACATTGGGTATCCTTTGATCAATCTTGTGAGATTGAAAGGTTTTCCTATTCTGCAGCAATTACAATTGGAAGAGCGCTTACTTCGAAACTCATCAGATAATTGGTGTATCATAAATGATGGAACAGATAATCCAACTGTTGTGGTGGGCATTTCTGGGAAACCTGATGAGCTCCTTGACATCAAATCTGTGTTAGGAGATCAAATTCCAGTCATCAGAAGGTTCACAGGGGGTGGAACTGTTATTGTTGATCACAACACAGTATTTGTCACCTTCATATGTAATAAAGATGCTGTTTCCGGACTAAAACCATTTCCGCAACCTATCATGTCCTGGAGTAGTCAACTATATAATAAAGTATTTCAAGGAACTGACTTTTATCTTCGGGAAAATGATTATGTCTTTGGTAACCGCAAGTTTGGTGGCAATGCTCAGTCCATTACTAAAAGCCGGTGGATCCACCATACATCTTTTCTGTGGGACTATGAGGTTAGGAATATGACATATCTGAAACATCCAAAACGGACTCCTGATTACCGTGAGGCAAGGAGCCATTTGGATTTTTTATGCAGCTTGAAGGACTACATGCCAAGACAGGTATTTATTGACAAAACAATAGAAGCAGTTGAAACTGAGTTTGCTACGAGTTTAAAGAAATTAAAAGAATGTGAATATTCTTTGACTGGAAACTACAGCCCCACAACAAGGATGCTGACAGAGCAAGAATTGGAAACAGCTGCAGGTTTATCACAAGATAATAAGCTTGACAAAGTCTTGGACATTGCATTATAACATCTTGGTATATGATTATTTCACCCCAAATATTAAATTTTACATGCCCCTCATTTATATTAATCTTGGAATCATTTCAAGTTTTAGCTTTATTTATTGCTTTGCAACCCCTTTCTCGCTTGGAATATTCTTGTTCTTGCCTGTTAACATTGAATTAGCATGGCCAGTCTAATTCGAGTCACAATGCACTGTTAGCCAACCCTCAAGGTATATTATATTTGTAGCTTCTTGAATTTCTAGATCGTAGAACGAGATTGCTTCAATTTGTGGTATATCCTAGACTGTCTTACAGATGCTAGGACATGTGTACGTCGTTATCAAACCAATTTTTGGGACTAGTAGTTAGTTTATCTATTAGAGCCTTCGCGGTGCAATTGAGTGGTGATAACTTGTGAAATTGGAAGGTTTGAGTATTGGTTCAATGTGTGAAGATGGATATCCTAGCTATCCTAGCTAGGGAGGGTGTATTTTTTCATAAGATGGAATCATGGAAGATGCAAGAACAATATTGTAGTTAACTTTCCGTTGTACAGGCGCAACCTTCTAGTTTTTGACGATTGCATCTCTCCACATCTCTCCCAACTCCCATCTTATTCTGTGGCCGATGAACACGGTGGATTCCTTTGATCGTCAAGTTGGGCAAGCCCTGCCTCAATTGTCTAGTCCTCTCGATCAATGATGAGGCCTCTTGTTAGTCGGCACCTCCTCACGGATCGTTTAAGGTGAACTATGGTGCGGCCTTCTTGAATGGTGATGTGGGTATTGGAGTCCTTGTTCCCGACTCAAATGGTGATGTTATGCTTGGTATGGAAAGTCACACTTCTATTGCAGGATCTGTTGTGCTCGTAGAGGCTATTGTCATATTTAATGGTTTGTTTAGAGCTCTTGAGATTTTTGTGGTTTCTATTCGATCTCCATCACAGTGGCTCTATCCATGACTTTCCTTTGGCCAACTGCAAGAATCATTATGTTGCTCATGAGTTAGCTATCAATACGCGAAGATTTGAATATTCAACAATTTAGCTTGTTAGTCC

Coding sequence (CDS)

ATGACGATGTATCAAACTAGAAACATTGGGTATCCTTTGATCAATCTTGTGAGATTGAAAGGTTTTCCTATTCTGCAGCAATTACAATTGGAAGAGCGCTTACTTCGAAACTCATCAGATAATTGGTGTATCATAAATGATGGAACAGATAATCCAACTGTTGTGGTGGGCATTTCTGGGAAACCTGATGAGCTCCTTGACATCAAATCTGTGTTAGGAGATCAAATTCCAGTCATCAGAAGGTTCACAGGGGGTGGAACTGTTATTGTTGATCACAACACAGTATTTGTCACCTTCATATGTAATAAAGATGCTGTTTCCGGACTAAAACCATTTCCGCAACCTATCATGTCCTGGAGTAGTCAACTATATAATAAAGTATTTCAAGGAACTGACTTTTATCTTCGGGAAAATGATTATGTCTTTGGTAACCGCAAGTTTGGTGGCAATGCTCAGTCCATTACTAAAAGCCGGTGGATCCACCATACATCTTTTCTGTGGGACTATGAGGTTAGGAATATGACATATCTGAAACATCCAAAACGGACTCCTGATTACCGTGAGGCAAGGAGCCATTTGGATTTTTTATGCAGCTTGAAGGACTACATGCCAAGACAGGTATTTATTGACAAAACAATAGAAGCAGTTGAAACTGAGTTTGCTACGAGTTTAAAGAAATTAAAAGAATGTGAATATTCTTTGACTGGAAACTACAGCCCCACAACAAGGATGCTGACAGAGCAAGAATTGGAAACAGCTGCAGGTTTATCACAAGATAATAAGCTTGACAAAGTCTTGGACATTGCATTATAA

Protein sequence

MTMYQTRNIGYPLINLVRLKGFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVVGISGKPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFICNKDAVSGLKPFPQPIMSWSSQLYNKVFQGTDFYLRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWDYEVRNMTYLKHPKRTPDYREARSHLDFLCSLKDYMPRQVFIDKTIEAVETEFATSLKKLKECEYSLTGNYSPTTRMLTEQELETAAGLSQDNKLDKVLDIAL

Homology

[Close]

BLAST of Bhi08G000968 vs. TAIR 10
Match: AT3G29010.1 (Biotin/lipoate A/B protein ligase family )

HSP 1 Score: 326.2 bits (835), Expect = 2.4e-89
Identity = 150/254 (59.06%), Postives = 198/254 (77.95%), Query Frame = 0


Query: 1   MTMYQTRNIGYPLINLVRLKGFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVVGISG 60
           M++ + +++  PL+NLVRLKG PIL+QL LEERLLR SSDNWCI+NDGT+ PT+V+G+SG
Sbjct: 1   MSLSRAKDLRLPLMNLVRLKGTPILEQLHLEERLLRTSSDNWCIVNDGTNVPTIVMGMSG 60

Query: 61  KPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFICNKDAVSGLKPFPQPIMSWS 120
           KP +LL++  V+ D+IPVI+RFTGGGTVIVD +T+FV+ ICNKD V  ++P+P+ +M+WS
Sbjct: 61  KPSQLLEVGPVMEDRIPVIKRFTGGGTVIVDKSTLFVSLICNKDDVPNVQPYPRSVMAWS 120

Query: 121 SQLYNKVFQGTD-FYLRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWDYEVRNMTYLKH 180
             LY++VF+  + F LRENDYVFG+RKFGGNAQSI K+RWIHHTSFLWDY+VRNM YLK 
Sbjct: 121 GSLYDEVFKSVNGFQLRENDYVFGDRKFGGNAQSIIKNRWIHHTSFLWDYDVRNMAYLKL 180

Query: 181 PKRTPDYREARSHLDFLCSLKDYMPRQVFIDKTIEAVETEFATSLKKLKECEYSLTGNYS 240
           P R P YR  R H DF+C +KDY+ R  F++KT++AV  +F      L++ +    G Y 
Sbjct: 181 PSRVPQYRLERDHTDFVCRMKDYIERSDFVEKTVKAVRNQFTLKQVNLEDIDSYAKGGYL 240

Query: 241 PTTRMLTEQELETA 254
            TTR+LT +ELE A
Sbjct: 241 KTTRLLTMEELEEA 254

[Close]

BLAST of Bhi08G000968 vs. ExPASy Swiss-Prot
Match: O07608 (Lipoate-protein ligase LplJ OS=Bacillus subtilis (strain 168) OX=224308 GN=lplJ PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 4.5e-08
Identity = 48/171 (28.07%), Postives = 74/171 (43.27%), Query Frame = 0


Query: 1   MTMYQTRNIGYPLINLVRLKGFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVVGISG 60
           M     +NI  P INL             +EE  +++       +    + P++++G + 
Sbjct: 1   MLFIDNQNINDPRINLA------------IEEYCVKHLDPEQQYLLFYVNQPSIIIGKNQ 60

Query: 61  KPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFICNKDAVS--GLKPFPQPIMS 120
              E ++ K V  + I V+RR +GGG V  D   +  +FI   D  S    K F +P++ 
Sbjct: 61  NTIEEINTKYVEENGIIVVRRLSGGGAVYHDLGNLNFSFITKDDGDSFHNFKKFTEPVIQ 120

Query: 121 WSSQLYNKVFQGTDFYLR-ENDYVFGNRKFGGNAQSITKSRWIHHTSFLWD 169
              QL      G +  L   ND V   RK  GNAQ  TK R   H + ++D
Sbjct: 121 ALHQL------GVEAELSGRNDIVVDGRKISGNAQFATKGRIFSHGTLMFD 153

[Close]

BLAST of Bhi08G000968 vs. ExPASy Swiss-Prot
Match: O13629 (Putative lipoate-protein ligase A OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=aim22 PE=3 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.1e-06
Identity = 40/155 (25.81%), Postives = 79/155 (50.97%), Query Frame = 0


Query: 28  LQLEERLLRNSSDNWCIINDGTDNPTVVVGISGKPDELLDIKSVLGDQIPVIRRFTGGGT 87
           L LE  L  NS+   C++   T++P+V++G +  P    ++K    + + +IRR +GGGT
Sbjct: 39  LALENYLYENSTAKHCLLL-YTNSPSVIIGRNQNPWVEANVKLCRDNFVNIIRRKSGGGT 98

Query: 88  VIVDHNTVFVTFICNKDAVSGLKPFPQPIMSWSS-QLYNKVFQGTDFYLRENDYVFGNRK 147
           V  D   +  + + N++  S  +     I +  +  ++ ++ Q  D  L ++      RK
Sbjct: 99  VFHDFGNLNYSVLMNREEFSHTENASIMIQALRNLGVHARLNQRHDIVLAQS-----QRK 158

Query: 148 FGGNAQSITKSRWIHHTSFLWDYEVRNM-TYLKHP 181
             G+A  I+++R  HH + L + ++  +  YL+ P
Sbjct: 159 ISGSAYKISRNRCYHHGTMLLNSDLEGVREYLRSP 187

[Close]

BLAST of Bhi08G000968 vs. ExPASy Swiss-Prot
Match: P47512 (Probable lipoate-protein ligase A OS=Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) OX=243273 GN=lplA PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.6e-05
Identity = 40/157 (25.48%), Postives = 69/157 (43.95%), Query Frame = 0


Query: 53  TVVVGISGKPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFICNKDAVSGLKPF 112
           T+VVG +      +++K +  D++ + RRF+GGG V  D   +  + I  +        +
Sbjct: 44  TIVVGRNQNTYAEVNLKELESDKVNLFRRFSGGGAVFHDLGNICFSIILPRTGKVMENAY 103

Query: 113 PQP----IMSWSSQLYNKVFQGTDFYLRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWD 172
            Q     +   +S     VF G       ND    N+KF G A+ I K R + H + L+D
Sbjct: 104 EQTTRNVVKFLNSLNVPAVFHG------RNDLEINNKKFSGLAEYIAKDRLLVHGTLLFD 163

Query: 173 YEVRNMT-YLKHPKRTPDYREARSHLDFLCSLKDYMP 205
            +   +  YL   K     +   S    + ++K+Y+P
Sbjct: 164 TDFSKLAKYLNVDKTKIASKGVDSVAKRVVNVKEYLP 194

[Close]

BLAST of Bhi08G000968 vs. ExPASy Swiss-Prot
Match: B3LQ66 (Putative lipoate-protein ligase A OS=Saccharomyces cerevisiae (strain RM11-1a) OX=285006 GN=AIM22 PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 3.6e-05
Identity = 46/200 (23.00%), Postives = 87/200 (43.50%), Query Frame = 0


Query: 3   MYQTRNIGY--------PLINLVRLKGFPILQQLQ--------LEERLLRNS------SD 62
           +Y   NIGY         L N+V+ KG  ++Q L         LE  + +N+       D
Sbjct: 89  LYNFYNIGYAKQIMSASQLENIVKAKGRFVIQSLSTSPYYNLALENYVFKNTPRAKRGPD 148

Query: 63  NWCIINDGTDNPTVVVGISGKPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFI 122
           N C +    ++   V+G +    + +D+  +      ++RRF+GGGTV+ D   V  +++
Sbjct: 149 N-CRLLFYINDRCAVIGKNQNLWQEVDLAKLKSKNFELLRRFSGGGTVLHDLGNVNYSYL 208

Query: 123 CNKDAVSGLKPFPQPIMSWSSQLYNKVFQGTDFYLRENDYVFGNRKFGGNAQSITKSRWI 181
            +++     K F + I+ W + L  ++    +      D +    K  G+A  I   +  
Sbjct: 209 TSREKFE-TKFFNKMIIKWLNSLNPELRLDLN---ERGDIIQDGFKISGSAYKIAGGKAY 268

[Close]

BLAST of Bhi08G000968 vs. ExPASy Swiss-Prot
Match: B5VLD2 (Putative lipoate-protein ligase A OS=Saccharomyces cerevisiae (strain AWRI1631) OX=545124 GN=AIM22 PE=3 SV=2)

HSP 1 Score: 50.4 bits (119), Expect = 3.6e-05
Identity = 46/200 (23.00%), Postives = 87/200 (43.50%), Query Frame = 0


Query: 3   MYQTRNIGY--------PLINLVRLKGFPILQQLQ--------LEERLLRNS------SD 62
           +Y   NIGY         L N+V+ KG  ++Q L         LE  + +N+       D
Sbjct: 89  LYNFYNIGYAKQIMSASQLENIVKAKGRFVIQSLSTSPYYNLALENYVFKNTPRAKRGPD 148

Query: 63  NWCIINDGTDNPTVVVGISGKPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFI 122
           N C +    ++   V+G +    + +D+  +      ++RRF+GGGTV+ D   V  +++
Sbjct: 149 N-CRLLFYINDRCAVIGKNQNLWQEVDLAKLKSKNFELLRRFSGGGTVLHDLGNVNYSYL 208

Query: 123 CNKDAVSGLKPFPQPIMSWSSQLYNKVFQGTDFYLRENDYVFGNRKFGGNAQSITKSRWI 181
            +++     K F + I+ W + L  ++    +      D +    K  G+A  I   +  
Sbjct: 209 TSREKFE-TKFFNKMIIKWLNSLNPELRLDLN---ERGDIIQDGFKISGSAYKIAGGKAY 268

[Close]

BLAST of Bhi08G000968 vs. ExPASy TrEMBL
Match: A0A1S3B7D4 (lipoate-protein ligase LplJ OS=Cucumis melo OX=3656 GN=LOC103486730 PE=4 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 3.8e-143
Identity = 250/271 (92.25%), Postives = 262/271 (96.68%), Query Frame = 0


Query: 1   MTMYQTRNIGYPLINLVRLKGFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVVGISG 60
           MTMYQTRNIG+PL+NLVRLKGFPILQQL LEERLLRNSSDNWCIINDGTDNPT+V+G+SG
Sbjct: 1   MTMYQTRNIGHPLMNLVRLKGFPILQQLHLEERLLRNSSDNWCIINDGTDNPTIVMGVSG 60

Query: 61  KPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFICNKDAVSGLKPFPQPIMSWS 120
           KPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFIC+KDAVSGLKPFPQPIMSWS
Sbjct: 61  KPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFICSKDAVSGLKPFPQPIMSWS 120

Query: 121 SQLYNKVFQGTDFYLRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWDYEVRNMTYLKHP 180
           S LYNKVFQGTDFYLRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWDYEVRNM YLKHP
Sbjct: 121 SLLYNKVFQGTDFYLRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWDYEVRNMAYLKHP 180

Query: 181 KRTPDYREARSHLDFLCSLKDYMPRQVFIDKTIEAVETEFATSLKKLKECEYSLTGNYSP 240
           KR PDYREARSHLDFLCSLK+YMPRQVFIDKT EA+ETEFATSLK+L+ECE SLTGNY+P
Sbjct: 181 KRVPDYREARSHLDFLCSLKEYMPRQVFIDKTTEAIETEFATSLKQLEECENSLTGNYNP 240

Query: 241 TTRMLTEQELETAAG-LSQDNKLDKVLDIAL 271
           TTRMLTEQELETAAG  SQD+KL KVLDIAL
Sbjct: 241 TTRMLTEQELETAAGCASQDSKLQKVLDIAL 271

[Close]

BLAST of Bhi08G000968 vs. ExPASy TrEMBL
Match: A0A5A7SYE8 (Lipoate-protein ligase LplJ OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00950 PE=4 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 3.8e-143
Identity = 250/271 (92.25%), Postives = 262/271 (96.68%), Query Frame = 0


Query: 1   MTMYQTRNIGYPLINLVRLKGFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVVGISG 60
           MTMYQTRNIG+PL+NLVRLKGFPILQQL LEERLLRNSSDNWCIINDGTDNPT+V+G+SG
Sbjct: 38  MTMYQTRNIGHPLMNLVRLKGFPILQQLHLEERLLRNSSDNWCIINDGTDNPTIVMGVSG 97

Query: 61  KPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFICNKDAVSGLKPFPQPIMSWS 120
           KPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFIC+KDAVSGLKPFPQPIMSWS
Sbjct: 98  KPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFICSKDAVSGLKPFPQPIMSWS 157

Query: 121 SQLYNKVFQGTDFYLRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWDYEVRNMTYLKHP 180
           S LYNKVFQGTDFYLRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWDYEVRNM YLKHP
Sbjct: 158 SLLYNKVFQGTDFYLRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWDYEVRNMAYLKHP 217

Query: 181 KRTPDYREARSHLDFLCSLKDYMPRQVFIDKTIEAVETEFATSLKKLKECEYSLTGNYSP 240
           KR PDYREARSHLDFLCSLK+YMPRQVFIDKT EA+ETEFATSLK+L+ECE SLTGNY+P
Sbjct: 218 KRVPDYREARSHLDFLCSLKEYMPRQVFIDKTTEAIETEFATSLKQLEECENSLTGNYNP 277

Query: 241 TTRMLTEQELETAAG-LSQDNKLDKVLDIAL 271
           TTRMLTEQELETAAG  SQD+KL KVLDIAL
Sbjct: 278 TTRMLTEQELETAAGCASQDSKLQKVLDIAL 308

[Close]

BLAST of Bhi08G000968 vs. ExPASy TrEMBL
Match: A0A0A0LSR7 (BPL/LPL catalytic domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G033280 PE=4 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.6e-141
Identity = 246/271 (90.77%), Postives = 262/271 (96.68%), Query Frame = 0


Query: 1   MTMYQTRNIGYPLINLVRLKGFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVVGISG 60
           M MYQTRNIG+PL+NLVRLKGFPILQQL+LEERLLRNSSDNWCIINDGTDNP +V+G+SG
Sbjct: 1   MAMYQTRNIGHPLMNLVRLKGFPILQQLKLEERLLRNSSDNWCIINDGTDNPAIVMGVSG 60

Query: 61  KPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFICNKDAVSGLKPFPQPIMSWS 120
           KPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFICNKDAVSGLKPFPQPIMSWS
Sbjct: 61  KPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFICNKDAVSGLKPFPQPIMSWS 120

Query: 121 SQLYNKVFQGTDFYLRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWDYEVRNMTYLKHP 180
           S +YNKVFQGTDFYLRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWDYEVRNM YLKHP
Sbjct: 121 SLVYNKVFQGTDFYLRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWDYEVRNMAYLKHP 180

Query: 181 KRTPDYREARSHLDFLCSLKDYMPRQVFIDKTIEAVETEFATSLKKLKECEYSLTGNYSP 240
           KR PDYREARSHLDFLCSLK+YMPRQVFIDKTIEA+ETEFATS K+L+ECE SLTGNY+P
Sbjct: 181 KRVPDYREARSHLDFLCSLKEYMPRQVFIDKTIEAIETEFATSSKQLEECENSLTGNYNP 240

Query: 241 TTRMLTEQELETAAG-LSQDNKLDKVLDIAL 271
           TTRMLTEQELETAAG +SQD++L+KV DIAL
Sbjct: 241 TTRMLTEQELETAAGCVSQDSELEKVSDIAL 271

[Close]

BLAST of Bhi08G000968 vs. ExPASy TrEMBL
Match: A0A6J1GC58 (uncharacterized protein LOC111452820 OS=Cucurbita moschata OX=3662 GN=LOC111452820 PE=4 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.3e-139
Identity = 242/271 (89.30%), Postives = 260/271 (95.94%), Query Frame = 0


Query: 1   MTMYQTRNIGYPLINLVRLKGFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVVGISG 60
           MT+YQTRNI +PLINLVRLKGFPIL+QLQLEERLLRNSSDNWCIINDGTDNPT+V+G+SG
Sbjct: 1   MTIYQTRNIRHPLINLVRLKGFPILRQLQLEERLLRNSSDNWCIINDGTDNPTIVMGVSG 60

Query: 61  KPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFICNKDAVSGLKPFPQPIMSWS 120
           KPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFICNKDAV+GLKPFPQPIMSWS
Sbjct: 61  KPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFICNKDAVAGLKPFPQPIMSWS 120

Query: 121 SQLYNKVFQGTDFYLRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWDYEVRNMTYLKHP 180
             LYNKVFQGTDF+LRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWDYEVRNM YL+HP
Sbjct: 121 GLLYNKVFQGTDFHLRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWDYEVRNMAYLRHP 180

Query: 181 KRTPDYREARSHLDFLCSLKDYMPRQVFIDKTIEAVETEFATSLKKLKECEYSLTGNYSP 240
           KR PDYREARSHLDF+CSLKDYMPRQVFIDKT EA+ET+FATSLK+L+ECEYS TGN SP
Sbjct: 181 KRVPDYREARSHLDFICSLKDYMPRQVFIDKTTEAIETQFATSLKQLEECEYSSTGNCSP 240

Query: 241 TTRMLTEQELETAAG-LSQDNKLDKVLDIAL 271
           TT++LTEQELETAAG +SQ NKL+KVL IAL
Sbjct: 241 TTKVLTEQELETAAGCVSQGNKLEKVLGIAL 271

[Close]

BLAST of Bhi08G000968 vs. ExPASy TrEMBL
Match: A0A6J1K8Z4 (uncharacterized protein LOC111493356 OS=Cucurbita maxima OX=3661 GN=LOC111493356 PE=4 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.4e-137
Identity = 239/271 (88.19%), Postives = 258/271 (95.20%), Query Frame = 0


Query: 1   MTMYQTRNIGYPLINLVRLKGFPILQQLQLEERLLRNSSDNWCIINDGTDNPTVVVGISG 60
           MT+YQT NI +PLINLVRLKGFPIL+QLQLEERLLRNSSDNWCIINDGTDNPT+V+G+SG
Sbjct: 1   MTIYQTSNIRHPLINLVRLKGFPILRQLQLEERLLRNSSDNWCIINDGTDNPTIVMGVSG 60

Query: 61  KPDELLDIKSVLGDQIPVIRRFTGGGTVIVDHNTVFVTFICNKDAVSGLKPFPQPIMSWS 120
           KPDELLDIKSVL DQIPVIRRFTGGGTVIVDHNTVFVTFICNKDAV+GLKPFPQPIMSWS
Sbjct: 61  KPDELLDIKSVLEDQIPVIRRFTGGGTVIVDHNTVFVTFICNKDAVAGLKPFPQPIMSWS 120

Query: 121 SQLYNKVFQGTDFYLRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWDYEVRNMTYLKHP 180
             LYNKVFQGTDF+LRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWDYEVRNMTYL+HP
Sbjct: 121 GLLYNKVFQGTDFHLRENDYVFGNRKFGGNAQSITKSRWIHHTSFLWDYEVRNMTYLRHP 180

Query: 181 KRTPDYREARSHLDFLCSLKDYMPRQVFIDKTIEAVETEFATSLKKLKECEYSLTGNYSP 240
           KR PDYREARSHLDF+CSLKDYMPRQ+FIDKT EA+ET+FATSLK+L+ECEYS TGN  P
Sbjct: 181 KRVPDYREARSHLDFICSLKDYMPRQIFIDKTTEAIETQFATSLKQLEECEYSSTGNCIP 240

Query: 241 TTRMLTEQELETAAG-LSQDNKLDKVLDIAL 271
           TT++LTEQELETAAG +SQ NKL+KVL IAL
Sbjct: 241 TTKVLTEQELETAAGCVSQGNKLEKVLGIAL 271

The following BLAST results are available for this feature:

BLAST of Bhi08G000968 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G29010.1	2.4e-89	59.06	Biotin/lipoate A/B protein ligase family	[more]

BLAST of Bhi08G000968 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O07608	4.5e-08	28.07	Lipoate-protein ligase LplJ OS=Bacillus subtilis (strain 168) OX=224308 GN=lplJ ...	[more]
O13629	1.1e-06	25.81	Putative lipoate-protein ligase A OS=Schizosaccharomyces pombe (strain 972 / ATC...	[more]
P47512	1.6e-05	25.48	Probable lipoate-protein ligase A OS=Mycoplasma genitalium (strain ATCC 33530 / ...	[more]
B3LQ66	3.6e-05	23.00	Putative lipoate-protein ligase A OS=Saccharomyces cerevisiae (strain RM11-1a) O...	[more]
B5VLD2	3.6e-05	23.00	Putative lipoate-protein ligase A OS=Saccharomyces cerevisiae (strain AWRI1631) ...	[more]

BLAST of Bhi08G000968 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B7D4	3.8e-143	92.25	lipoate-protein ligase LplJ OS=Cucumis melo OX=3656 GN=LOC103486730 PE=4 SV=1	[more]
A0A5A7SYE8	3.8e-143	92.25	Lipoate-protein ligase LplJ OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A0A0LSR7	1.6e-141	90.77	BPL/LPL catalytic domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G...	[more]
A0A6J1GC58	3.3e-139	89.30	uncharacterized protein LOC111452820 OS=Cucurbita moschata OX=3662 GN=LOC1114528...	[more]
A0A6J1K8Z4	2.4e-137	88.19	uncharacterized protein LOC111493356 OS=Cucurbita maxima OX=3661 GN=LOC111493356...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 14..262 e-value: 9.3E-43 score: 148.5
None	No IPR available	PANTHER	PTHR43506	BIOTIN/LIPOATE A/B PROTEIN LIGASE FAMILY	coord: 2..255
None	No IPR available	PANTHER	PTHR43506:SF1	BIOTIN/LIPOATE A/B PROTEIN LIGASE FAMILY	coord: 2..255
None	No IPR available	CDD	cd16443	LplA	coord: 16..220 e-value: 2.09033E-45 score: 150.098
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 15..232
IPR004143	Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domain	PROSITE	PS51733	BPL_LPL_CATALYTIC	coord: 39..224 score: 12.733753

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi08M000968	Bhi08M000968	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006464	cellular protein modification process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0046983	protein dimerization activity