Bhi08G000828 (gene) Wax gourd (B227) v1

Overview
NameBhi08G000828
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionberberine bridge enzyme-like 18
Locationchr8: 32198889 .. 32201761 (-)
RNA-Seq ExpressionBhi08G000828
SyntenyBhi08G000828
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAAACCATGAAGTTCTCTTCTCCATTAATCCCTCTTGCTCTTGTCCTTATTGTTCTATATTCATGTCCATTATGGGCTGCAGCATCTCCCCAAAAATATCAACACTTTCTTCAATGTCTCTCAGAGCATTCTTCAAACAGTTATCCCATTTCCAAAGTGGTTTATAGTCCAATCAACTCATCTTATTCTTCTGTTTTGGACTTCTCCATTAAAAACCTCAGATTCTCAAAGCCTCAAACCCCAAAGCCACTCCTTATTATCACACCCTCACATGTTTCTCACATTCAAGCAGCAGTCATCTGCTCCAAATCTCATGGCTTACAAATCCGAACACGGAGCGGCGGCCATGACTTCGAGGGTCTTTCTTACGTTGCCTATCGTCCATTTATCGTCGTTGACCTAATAAATCTCAGGTCTATTTCTGTTTCTTTGTTTGTTTTTCTTTTCTCGAGTATAATAAATTGGAGTATGAGTACATATCAATTATCACTAATCTAAGTTCATTTAGACTCACGTTTGTTATTATTTATAACAGGGTATACGGTAGTGTATTTCAATATTACCGTTGAACTATACTTACGTTTACTTAAGTTTATTCATGTTTGTTAATTTTTCGAGTATAATAAATGGAGGTATGATATGTCAATTATAGTTAGAGAAATGTTCATTTTTTGCATAGGTATGCTATTTGTTTTTTTTTAGTATAGTAAGTTAGGGTATGACTAGTACATGGTCAATAACAATTGAATTATATTCACTTGGGCTCAGGTTTTAAACTGATAATTTGGTTTTTTCTCTAACAAGTGTGGGTATGAGACCCTATATATCAATTATAGCTGAGTTATATATTCACTTTGACTCAGATCTGTTAAGGTTGACGATGAAAACAACACTGCGTGGGTTGAGTCAGGTGCAACTCTAGGTGAACTTTACTACAGAATTGGTGAGAAAAGTCGAACCTTGGCGTTCCCGGCAGGTGTTTGTCCGACGGTCGGCATTGGTGGGCACTTCAGCGGCGGTGGATATGGATTGATGCTGAGGAAATTTGGTCTTGCTGCTGATAATGTGATAGATGCTCATTTGGTTGATGCTAATGGGAAGGTTCATGATAGAGAGTCAATGGGAGAGGATTTGTTTTGGGCCATTAGAGGTGGCGGCGGAGGGAGCTTTGGGATTGTGGTGGCGTGGAAGATCAAGCTGGTTCCGGTTCCGGCAACCGTGACAATTTGCTCGACACAAAGAAATTTGGAGGAAGATGCAATTAAACTGGTCCATCGCTGGCAATATGTGGCTAACAAATTAGATGAAAATCTATACCTTGGCATCATTTTGACTGGTATTATATTCCTTCTAAAGTCTATGGTTGAGTTTTTAATTATTATTGTTTCTTTCTTTCTTTTCTTTGGAAAATATGTTTGTTAAATTAATATTATAATGTGAAGTAAACCACACGACATGATACATATATATATATTCCTAGACTGAGAGTAAAATATGTAGATGTAGAAAAATAAAAGAGTAGAAGGAGGAAATTTGTTAGAAACAGCAAATCTAGAGATATAGAAATATATATATTTTCAGTGGAGGAAAGTTAAAATAGTCGGAAATATTTTAGTAAGAGAGTATTAGCTAATGTTTTCCAGACATGACGTGAGAGAGAGAAAAGTCCAATTTTAGAGAGAAAATAATAGAGAGCGTTGAGGATCTCACATTAAAAAAACGATGAGAGATCTCATTATCTTATATATCTATCGAGCTATTCTCAAATTTTAAAGAAATATAATAATTGACAATTTATATTAATTATATAGCACCAAAATCATATTAGGATGGCAAAACTTTCCTACATTCAAATACAAAGTTGTTCATAAAATGAAACAAAATAAAACTTCCAACTTCCAAAACAAACTCACCCCAGGAATCAACTAATGAAGTAAGAAATAAGAGATGAAGTTTTTTTTCCTCTCCCCAATAAACATAAGAATGATCTGAACTCAGAATTCTCAATCATATATGTAGGTGGGAAAGCTTCAACTCAAGGAGGCATTACTAACCCAACAGCTACATTCTTCTCATTATTTCTTGGAAGGATAGATGAACTTTTGGCCACTTTGTCAACAACTTTTCCTGAGTTAGGTTTAATAAAACAAGATTGTGTCGAAGCGACTTGGGTTGAGTCAACCCTCATTATCGCCACAGGGGTTCAACCCATTGAGTCCTTAGAACCTTTACTCAATAGAACACCTCCCACAATCGAAAACACAAAAATAAAATCCGACTACGTCAAGGAACCCCTTCCCGAGGCTGCAATCGAGGGCATATGCCAAAGATTAAAGGCTCAAGATATAGAAACATCACAACTCATATTTGTTCCTTATGGAGGGAAAATGAGTCAAATTTCTGAGTCAGAAACCCCATTTTCACATAGAGCTGGAAATTTGTACAAGATTGGGTACGTTGTAGGGTGGAAGGAACAAAGTTTAAAGGCAAAGAAAAGGCACATACATTGGATACGAGAGGTTTACGAGTACATGATTCCTTTTGTTTCAAAATCACCCAGGGCTGCATATGCAAATTACAGAGATCTTGACATTGGAGCTAACAACAAATATGGACAGACAAGCGTCGAGCAAGCAAGCGTTTGGGGTTTGAAGTATTTTGGGAACAATTTTAAGAAGTTGGTGTATGTTAAAACTAAGGTTGATCCTTATGATTTCTTCAGGCATGAACAAAGTATACCTACCCTCTAACTAATGTAATATTTAGTGATAAAACATTAATATTTAGATTTCAAGCATTTTCAAACTATCCTCCCAAATCCCTGTTGGTAGTAAAATAAAGGCTTATGTTGTGAGTACTTTATTTATATCC

mRNA sequence

CCAAAACCATGAAGTTCTCTTCTCCATTAATCCCTCTTGCTCTTGTCCTTATTGTTCTATATTCATGTCCATTATGGGCTGCAGCATCTCCCCAAAAATATCAACACTTTCTTCAATGTCTCTCAGAGCATTCTTCAAACAGTTATCCCATTTCCAAAGTGGTTTATAGTCCAATCAACTCATCTTATTCTTCTGTTTTGGACTTCTCCATTAAAAACCTCAGATTCTCAAAGCCTCAAACCCCAAAGCCACTCCTTATTATCACACCCTCACATGTTTCTCACATTCAAGCAGCAGTCATCTGCTCCAAATCTCATGGCTTACAAATCCGAACACGGAGCGGCGGCCATGACTTCGAGGGTCTTTCTTACGTTGCCTATCGTCCATTTATCGTCGTTGACCTAATAAATCTCAGATCTGTTAAGGTTGACGATGAAAACAACACTGCGTGGGTTGAGTCAGGTGCAACTCTAGGTGAACTTTACTACAGAATTGGTGAGAAAAGTCGAACCTTGGCGTTCCCGGCAGGTGTTTGTCCGACGGTCGGCATTGGTGGGCACTTCAGCGGCGGTGGATATGGATTGATGCTGAGGAAATTTGGTCTTGCTGCTGATAATGTGATAGATGCTCATTTGGTTGATGCTAATGGGAAGGTTCATGATAGAGAGTCAATGGGAGAGGATTTGTTTTGGGCCATTAGAGGTGGCGGCGGAGGGAGCTTTGGGATTGTGGTGGCGTGGAAGATCAAGCTGGTTCCGGTTCCGGCAACCGTGACAATTTGCTCGACACAAAGAAATTTGGAGGAAGATGCAATTAAACTGGTCCATCGCTGGCAATATGTGGCTAACAAATTAGATGAAAATCTATACCTTGGCATCATTTTGACTGGTGGGAAAGCTTCAACTCAAGGAGGCATTACTAACCCAACAGCTACATTCTTCTCATTATTTCTTGGAAGGATAGATGAACTTTTGGCCACTTTGTCAACAACTTTTCCTGAGTTAGGTTTAATAAAACAAGATTGTGTCGAAGCGACTTGGGTTGAGTCAACCCTCATTATCGCCACAGGGGTTCAACCCATTGAGTCCTTAGAACCTTTACTCAATAGAACACCTCCCACAATCGAAAACACAAAAATAAAATCCGACTACGTCAAGGAACCCCTTCCCGAGGCTGCAATCGAGGGCATATGCCAAAGATTAAAGGCTCAAGATATAGAAACATCACAACTCATATTTGTTCCTTATGGAGGGAAAATGAGTCAAATTTCTGAGTCAGAAACCCCATTTTCACATAGAGCTGGAAATTTGTACAAGATTGGGTACGTTGTAGGGTGGAAGGAACAAAGTTTAAAGGCAAAGAAAAGGCACATACATTGGATACGAGAGGTTTACGAGTACATGATTCCTTTTGTTTCAAAATCACCCAGGGCTGCATATGCAAATTACAGAGATCTTGACATTGGAGCTAACAACAAATATGGACAGACAAGCGTCGAGCAAGCAAGCGTTTGGGGTTTGAAGTATTTTGGGAACAATTTTAAGAAGTTGGTGTATGTTAAAACTAAGGTTGATCCTTATGATTTCTTCAGGCATGAACAAAGTATACCTACCCTCTAACTAATGTAATATTTAGTGATAAAACATTAATATTTAGATTTCAAGCATTTTCAAACTATCCTCCCAAATCCCTGTTGGTAGTAAAATAAAGGCTTATGTTGTGAGTACTTTATTTATATCC

Coding sequence (CDS)

ATGAAGTTCTCTTCTCCATTAATCCCTCTTGCTCTTGTCCTTATTGTTCTATATTCATGTCCATTATGGGCTGCAGCATCTCCCCAAAAATATCAACACTTTCTTCAATGTCTCTCAGAGCATTCTTCAAACAGTTATCCCATTTCCAAAGTGGTTTATAGTCCAATCAACTCATCTTATTCTTCTGTTTTGGACTTCTCCATTAAAAACCTCAGATTCTCAAAGCCTCAAACCCCAAAGCCACTCCTTATTATCACACCCTCACATGTTTCTCACATTCAAGCAGCAGTCATCTGCTCCAAATCTCATGGCTTACAAATCCGAACACGGAGCGGCGGCCATGACTTCGAGGGTCTTTCTTACGTTGCCTATCGTCCATTTATCGTCGTTGACCTAATAAATCTCAGATCTGTTAAGGTTGACGATGAAAACAACACTGCGTGGGTTGAGTCAGGTGCAACTCTAGGTGAACTTTACTACAGAATTGGTGAGAAAAGTCGAACCTTGGCGTTCCCGGCAGGTGTTTGTCCGACGGTCGGCATTGGTGGGCACTTCAGCGGCGGTGGATATGGATTGATGCTGAGGAAATTTGGTCTTGCTGCTGATAATGTGATAGATGCTCATTTGGTTGATGCTAATGGGAAGGTTCATGATAGAGAGTCAATGGGAGAGGATTTGTTTTGGGCCATTAGAGGTGGCGGCGGAGGGAGCTTTGGGATTGTGGTGGCGTGGAAGATCAAGCTGGTTCCGGTTCCGGCAACCGTGACAATTTGCTCGACACAAAGAAATTTGGAGGAAGATGCAATTAAACTGGTCCATCGCTGGCAATATGTGGCTAACAAATTAGATGAAAATCTATACCTTGGCATCATTTTGACTGGTGGGAAAGCTTCAACTCAAGGAGGCATTACTAACCCAACAGCTACATTCTTCTCATTATTTCTTGGAAGGATAGATGAACTTTTGGCCACTTTGTCAACAACTTTTCCTGAGTTAGGTTTAATAAAACAAGATTGTGTCGAAGCGACTTGGGTTGAGTCAACCCTCATTATCGCCACAGGGGTTCAACCCATTGAGTCCTTAGAACCTTTACTCAATAGAACACCTCCCACAATCGAAAACACAAAAATAAAATCCGACTACGTCAAGGAACCCCTTCCCGAGGCTGCAATCGAGGGCATATGCCAAAGATTAAAGGCTCAAGATATAGAAACATCACAACTCATATTTGTTCCTTATGGAGGGAAAATGAGTCAAATTTCTGAGTCAGAAACCCCATTTTCACATAGAGCTGGAAATTTGTACAAGATTGGGTACGTTGTAGGGTGGAAGGAACAAAGTTTAAAGGCAAAGAAAAGGCACATACATTGGATACGAGAGGTTTACGAGTACATGATTCCTTTTGTTTCAAAATCACCCAGGGCTGCATATGCAAATTACAGAGATCTTGACATTGGAGCTAACAACAAATATGGACAGACAAGCGTCGAGCAAGCAAGCGTTTGGGGTTTGAAGTATTTTGGGAACAATTTTAAGAAGTTGGTGTATGTTAAAACTAAGGTTGATCCTTATGATTTCTTCAGGCATGAACAAAGTATACCTACCCTCTAA

Protein sequence

MKFSSPLIPLALVLIVLYSCPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSSYSSVLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFIVVDLINLRSVKVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKASTQGGITNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGVQPIESLEPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQISESETPFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYANYRDLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL
Homology
BLAST of Bhi08G000828 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 535.4 bits (1378), Expect = 5.2e-152
Identity = 269/533 (50.47%), Postives = 376/533 (70.54%), Query Frame = 0

Query: 3   FSSPLIPLALVLIVLYSCPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSSYSS 62
           FSS LI      ++L S P   +A+      FLQCLS   ++S  +SKV+++P ++S+SS
Sbjct: 7   FSSVLIFFTTSTLLL-SIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSS 66

Query: 63  VLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYV 122
           VL  SI+N RFS P  PKP+LI+TP   S +Q+AV C++  G+ IRTRSGGHD+EGLSYV
Sbjct: 67  VLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYV 126

Query: 123 AYRPFIVVDLINLRSVKVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIG 182
            ++PF+++DL NLRS+ VD +N + WV++GAT+GELYY IG+K+RTLAFPAGVCPTVG+G
Sbjct: 127 THKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVG 186

Query: 183 GHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVV 242
           GHFSGGGYG +LRK GLAAD+VIDA +VDA G++ +R  MGED FWAIRGGGG SF +V+
Sbjct: 187 GHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVL 246

Query: 243 AWKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKASTQGG 302
           +WKI L+ VP+TVT+ +  +  E+ A+K++HRWQ+VA+K+ ++L++ ++L   K   +  
Sbjct: 247 SWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYKNMVR-- 306

Query: 303 ITNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIA-TGVQPIESL 362
                A+F  L+LG +  LL  ++  FPELGL + DC E +W+ES +  A  G +PI   
Sbjct: 307 -----ASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELGEEPI--- 366

Query: 363 EPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQIS 422
             L  RT  ++   K KSD+V+EP+P+ AI  + +RL+  + E +QLIF P+GGKMS+I+
Sbjct: 367 NVLTKRTRASLA-FKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIA 426

Query: 423 ESETPFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYANYR 482
           + ETPF HR GN+Y+I Y+  W+      K++++ W+  VY+ M  FV+KSPR AY N R
Sbjct: 427 DYETPFPHRKGNIYEIQYLNYWRGD---VKEKYMRWVERVYDDMSEFVAKSPRGAYINLR 486

Query: 483 DLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIP 535
           DLD+G      ++  E+   WG+KYF NNF++LV VKT VDP DFF  EQSIP
Sbjct: 487 DLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Bhi08G000828 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 524.2 bits (1349), Expect = 1.2e-148
Identity = 261/538 (48.51%), Postives = 380/538 (70.63%), Query Frame = 0

Query: 1   MKFSSPLIPLALVLIVLYSCPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSSY 60
           M+FSS L    ++L  L   PL  +A    ++ FL+CLS   +++    KV+++  +SS+
Sbjct: 1   MEFSSFL--FTILLFSLNISPL-VSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSF 60

Query: 61  SSVLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120
            S+LD SI+N RFS  +TPKP+ IITP   S +Q  + C++ HG+ +RTRS GH +EGLS
Sbjct: 61  FSILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLS 120

Query: 121 YVAY-RPFIVVDLINLRSVKVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180
           Y+AY +PF V+DL NLRS+ +D +N T WV++GAT GELYY IG+ +++LAFPAG+ PTV
Sbjct: 121 YIAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTV 180

Query: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFG 240
           G+GG FSGGGYG +LRK+GLAADN+IDA +VDA+G++ DR++MGED FWAIRGGGG SFG
Sbjct: 181 GVGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFG 240

Query: 241 IVVAWKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKAST 300
           ++++WK+KLV VP+T+T+   Q+  +++A++++ +WQY A+K+ ++L++   L   + S 
Sbjct: 241 VILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTL---ERSN 300

Query: 301 QGGITNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGVQPIE 360
           +  +    A F  L++G ++ LLA +   FPELGL K+ C E +W+ES L  A   +  E
Sbjct: 301 KNAV---HALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKG-E 360

Query: 361 SLEPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQ 420
           SL  L NR   ++ + K K D+V+EP+PEAAI+ I +RL+A +    ++I  P+GGKMS+
Sbjct: 361 SLGVLTNRERTSL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSE 420

Query: 421 ISESETPFSHRAGNLYKIGYVVGWKEQSLKAK---KRHIHWIREVYEYMIPFVSKSPRAA 480
           ++E ETPF HR GNLY+I YV  W+E+  K K    +++ W+  VYE+M P+VSKSPR A
Sbjct: 421 MAEYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGA 480

Query: 481 YANYRDLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIP 535
           Y N++D+D+G      +T  E+   WG+KYF NNF++LV VKT+VDP DFF  EQSIP
Sbjct: 481 YVNFKDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of Bhi08G000828 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 519.6 bits (1337), Expect = 2.9e-147
Identity = 260/532 (48.87%), Postives = 367/532 (68.98%), Query Frame = 0

Query: 11  ALVLIVLYSCPLW-AAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSSYSSVLDFSIK 70
           AL+L++ +   +W +++S    + F QCL+ +S   +PIS  ++   N SYSSVL  +I+
Sbjct: 4   ALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIR 63

Query: 71  NLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAY--RPF 130
           NLRF+   TPKP LII  +H SH+QAA+ C K H LQ++ RSGGHD++GLSYV Y  +PF
Sbjct: 64  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 123

Query: 131 IVVDLINLRSVKVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHFSG 190
            V+D+ NLRSV VD  + TAWV++GA LGE+YY I EKS+TLA+PAG+CPTVG+GGH SG
Sbjct: 124 FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 183

Query: 191 GGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKIK 250
           GGYG M+RK+GL  DN IDA +VD NGK+ DR+ MGEDL+WAI GGGGGS+G+V+A+KI 
Sbjct: 184 GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 243

Query: 251 LVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIIL--TGGKASTQGGITN 310
           LV VP  VT+    R LE++A  ++HRWQ VA KL + L++  ++    G  S+Q  +  
Sbjct: 244 LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTV-- 303

Query: 311 PTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGVQPIESLEPLL 370
              TF ++FLG    LL+ L+  FPELGL++ DC E +W++S L   T +Q   S   LL
Sbjct: 304 -RTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVL-FWTNIQVGSSETLLL 363

Query: 371 NRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQISESET 430
            R  P +   K KSDYV+EP+    +E I +++   +I T  + F PYGG+M +IS + T
Sbjct: 364 QRNQP-VNYLKRKSDYVREPISRTGLESIWKKMIELEIPT--MAFNPYGGEMGRISSTVT 423

Query: 431 PFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYANYRDLDI 490
           PF +RAGNL+KI Y   W++++L    R++   R++Y++M PFVSK+PR ++ NYRD+D+
Sbjct: 424 PFPYRAGNLWKIQYGANWRDETL--TDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDL 483

Query: 491 GANNKYGQ-TSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 537
           G N+  G+ +S  +   +G KYF  NF++LV +KT+VD  +FFR+EQSIP L
Sbjct: 484 GINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of Bhi08G000828 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 515.4 bits (1326), Expect = 5.6e-146
Identity = 261/545 (47.89%), Postives = 369/545 (67.71%), Query Frame = 0

Query: 1   MKFSSPLIPLALVLIVLYS----CPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPI 60
           M  S PL PL  +L++ +S     P  + AS Q    F+ C+  ++   +P+ K  ++P 
Sbjct: 1   MGISKPL-PLFSILVLYFSLYTITPTSSLASLQ--DQFINCVQRNTHVYFPLEKTFFAPT 60

Query: 61  N--SSYSSVLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGH 120
              S +S VL+ + +NLRF K   PKP  I +P H SH+QA++ICSK   + +R RSGGH
Sbjct: 61  KNVSMFSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGH 120

Query: 121 DFEGLSYVAY--RPFIVVDLINLRSVKVDDENNTAWVESGATLGELYYRIGEKSRTLAFP 180
           D+EGLSYV+   +PFI++DL  +R V ++ ++N+AWV+SGAT+GELYYRI EKS+   FP
Sbjct: 121 DYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFP 180

Query: 181 AGVCPTVGIGGHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRG 240
           AG+C ++GIGGH +GG YG M+RK+GL ADNV+DA +VDANGK+ DR +MGED FWAIRG
Sbjct: 181 AGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRG 240

Query: 241 GGGGSFGIVVAWKIKLVPVPATVTICSTQRNLEED-AIKLVHRWQYVANKLDENLYLGII 300
           G GGSFGI++AWKIKLVPVP TVT+ +  + L++D   K++ +WQ VA+KL E L++ ++
Sbjct: 241 GAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVL 300

Query: 301 LTGGKASTQGGITNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLII 360
                 +  GG    T ++ +LFLG    L+  +  +FPELGL  +DC+E +W+ES   I
Sbjct: 301 F---NVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYI 360

Query: 361 ATGVQPIESLEPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFV 420
           +       +   L  ++P    + K KSD+VK P+PE+ ++GI ++L  +DI    +I+ 
Sbjct: 361 SGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWN 420

Query: 421 PYGGKMSQISESETPFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSK 480
           PYGG M++I ES+ PF HR G L+K+ YV  W + S K   RHI+WIR++Y YM P+VS 
Sbjct: 421 PYGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSS 480

Query: 481 SPRAAYANYRDLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQ 537
           +PR AY NYRDLD+G N K  +T ++QA VWG  YF NNF +L+ +K KVDP +FFRHEQ
Sbjct: 481 NPREAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQ 536

BLAST of Bhi08G000828 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 512.7 bits (1319), Expect = 3.6e-145
Identity = 255/530 (48.11%), Postives = 369/530 (69.62%), Query Frame = 0

Query: 10  LALVLIVLYSCPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSS--YSSVLDFS 69
           L LV ++L S PL   +S    Q F++CL ++S  S+PI+   +SP  ++  +   L+ +
Sbjct: 12  LFLVTLLLISVPL---SSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELEST 71

Query: 70  IKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVA--YR 129
            +NLR+  P  PKP+ I  P + +H+QAAV+C+K   L +R RSGGHD+EGLS+VA    
Sbjct: 72  AQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDET 131

Query: 130 PFIVVDLINLRSVKVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHF 189
           PF++VDL  LR V VD ++N+AW  +GAT+GE+YYRI EKS+T  FPAG+C ++GIGGH 
Sbjct: 132 PFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHL 191

Query: 190 SGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWK 249
            GG YG M+RKFGL ADNV+DA +VDANG++ DR +MGED+FWAIRGGGGGSFG+++AWK
Sbjct: 192 VGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWK 251

Query: 250 IKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKASTQGGITN 309
           IKLVPVPATVT+ +  + LE+D  K++++W+ +A+KLD++L++ +I++    +T+ G   
Sbjct: 252 IKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRT 311

Query: 310 PTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGVQPIESLEPLL 369
            + ++ + FLG  + LL  +  +FPELGL K+DC E +W++S + IA G     + E LL
Sbjct: 312 ISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIA-GFPNSAAPEALL 371

Query: 370 NRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQISESET 429
                   + K KSD+VKEP+P   +EG+ +R   +D  +   I+ PYGG MS+ISESE 
Sbjct: 372 AGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEED--SPLTIWNPYGGMMSRISESEI 431

Query: 430 PFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYANYRDLDI 489
           PF HR G L+KI ++  W++  + +++RH+ WIRE+Y YM  +VSK+PR AY NYRDLD+
Sbjct: 432 PFPHRNGTLFKIQWLSTWQDGKV-SEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDL 491

Query: 490 GANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPT 536
           G N   G+T   +   WG KY+  NF++LV +K + DP +FFRHEQS+PT
Sbjct: 492 GTNE--GETDARE---WGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of Bhi08G000828 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 7.3e-151
Identity = 269/533 (50.47%), Postives = 376/533 (70.54%), Query Frame = 0

Query: 3   FSSPLIPLALVLIVLYSCPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSSYSS 62
           FSS LI      ++L S P   +A+      FLQCLS   ++S  +SKV+++P ++S+SS
Sbjct: 7   FSSVLIFFTTSTLLL-SIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSS 66

Query: 63  VLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYV 122
           VL  SI+N RFS P  PKP+LI+TP   S +Q+AV C++  G+ IRTRSGGHD+EGLSYV
Sbjct: 67  VLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYV 126

Query: 123 AYRPFIVVDLINLRSVKVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIG 182
            ++PF+++DL NLRS+ VD +N + WV++GAT+GELYY IG+K+RTLAFPAGVCPTVG+G
Sbjct: 127 THKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVG 186

Query: 183 GHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVV 242
           GHFSGGGYG +LRK GLAAD+VIDA +VDA G++ +R  MGED FWAIRGGGG SF +V+
Sbjct: 187 GHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVL 246

Query: 243 AWKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKASTQGG 302
           +WKI L+ VP+TVT+ +  +  E+ A+K++HRWQ+VA+K+ ++L++ ++L   K   +  
Sbjct: 247 SWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYKNMVR-- 306

Query: 303 ITNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIA-TGVQPIESL 362
                A+F  L+LG +  LL  ++  FPELGL + DC E +W+ES +  A  G +PI   
Sbjct: 307 -----ASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELGEEPI--- 366

Query: 363 EPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQIS 422
             L  RT  ++   K KSD+V+EP+P+ AI  + +RL+  + E +QLIF P+GGKMS+I+
Sbjct: 367 NVLTKRTRASLA-FKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIA 426

Query: 423 ESETPFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYANYR 482
           + ETPF HR GN+Y+I Y+  W+      K++++ W+  VY+ M  FV+KSPR AY N R
Sbjct: 427 DYETPFPHRKGNIYEIQYLNYWRGD---VKEKYMRWVERVYDDMSEFVAKSPRGAYINLR 486

Query: 483 DLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIP 535
           DLD+G      ++  E+   WG+KYF NNF++LV VKT VDP DFF  EQSIP
Sbjct: 487 DLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Bhi08G000828 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 3.1e-149
Identity = 261/512 (50.98%), Postives = 355/512 (69.34%), Query Frame = 0

Query: 32  QHFLQCLSEHSSNSYPISKVVYSPINSSYSSVLDFSIKNLRFSKPQTPKPLLIITPSHVS 91
           ++FL+C S+H  N+    K+VY+  +  Y S+L+ +I+NLRF    TPKPL+I+TPS+ S
Sbjct: 32  ENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNS 91

Query: 92  HIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFIVVDLINLRSVKVDDENNTAWVES 151
           HIQA ++CSK  GLQIRTRSGGHD EG+SY++  PF+VVDL N+ S+K+D  + TAWVE+
Sbjct: 92  HIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEA 151

Query: 152 GATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHFSGGGYGLMLRKFGLAADNVIDAHLVD 211
           GATLGE+YY I EK+  L+FP G CPTVG+GGHFSGGGYG ++R +GLAADN+IDAHLV+
Sbjct: 152 GATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 212 ANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKIKLVPVPATVTICSTQRNLE-EDAIK 271
            +GKV DR+SMGEDLFWAIRGGGG +FGI+ AWKIKLV VP+  TI S ++N+E    +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVK 271

Query: 272 LVHRWQYVANKLDENLYLGIILTGGKASTQGGITNPT--ATFFSLFLGRIDELLATLSTT 331
           L ++WQ +A K D++L L         +   G    T    F S+F G +D L+  ++ +
Sbjct: 272 LFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKS 331

Query: 332 FPELGLIKQDCVEATWVESTLIIATGVQPIESL----EPLLNRTPPTIENTKIKSDYVKE 391
           FPELG+ K DC E +W+++T I  +GV    +     E LL+R+        IK DYVK+
Sbjct: 332 FPELGIKKTDCKEFSWIDTT-IFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKK 391

Query: 392 PLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQISESETPFSHRAGNLYKIGYVVGWK 451
           P+PE A+  I ++L  +D+     +  PYGG M +ISES  PF HRAG +Y++ Y   W+
Sbjct: 392 PIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWE 451

Query: 452 EQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYANYRDLDIGANNKYGQTSVEQASVWGL 511
           +Q  +  ++HI+W+R VY +  P+VS++PR AY NYRDLD+G  N     +  QA +WG 
Sbjct: 452 KQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIWGE 511

Query: 512 KYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 537
           KYFG NF +LV VKTKVDP +FFR+EQSIP L
Sbjct: 512 KYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of Bhi08G000828 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 8.9e-149
Identity = 264/518 (50.97%), Postives = 363/518 (70.08%), Query Frame = 0

Query: 26  ASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSSYSSVLDFSIKNLRFSKPQTPKPLLII 85
           A+PQ  ++FL+C S++   +   +K+VY+  +  Y S+L+ +I+NLRF+   TPKPL+II
Sbjct: 28  ANPQ--ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVII 87

Query: 86  TPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFIVVDLINLRSVKVDDENN 145
           TP +VSHIQ  ++CSK  GLQIRTRSGGHD EG+SY++  PF++VDL N+ SVK+D  + 
Sbjct: 88  TPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQ 147

Query: 146 TAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHFSGGGYGLMLRKFGLAADNVI 205
           TAWVE+GATLGE+YY I E +  L+FPAG CPTVG GGHFSGGGYG ++R +GLAADN+I
Sbjct: 148 TAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNII 207

Query: 206 DAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKIKLVPVPATVTICSTQRNLE 265
           DAHLV+ +GKV DR+SMGEDLFWAIRGGGG +FGI+ AWKI+LV VP+  TI S ++N+E
Sbjct: 208 DAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNME 267

Query: 266 -EDAIKLVHRWQYVANKLDENLYLGI-ILTGGKASTQG-GITNPTATFFSLFLGRIDELL 325
             + +KLV++WQ +A   ++ L L    +T      QG   T   + F S+F G +D L+
Sbjct: 268 IHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLV 327

Query: 326 ATLSTTFPELGLIKQDCVEATWVESTLIIATGVQPIESL----EPLLNRTPPTIENTKIK 385
             ++ +FPELG+ K DC + +W++ T+I  +GV    +     E LL+R+        IK
Sbjct: 328 DLMNKSFPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIK 387

Query: 386 SDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQISESETPFSHRAGNLYKIG 445
            DYVK+P+PE A+  I ++L  +D+     +F PYGG M +ISES  PF HRAG +Y+I 
Sbjct: 388 LDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIW 447

Query: 446 YVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYANYRDLDIGANNKYGQTSVEQ 505
           Y+  W++Q  +  ++HI+WIR VY +  P+VS++PR AY NYRDLD+G  N     +  Q
Sbjct: 448 YIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQ 507

Query: 506 ASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 537
           A +WG KYFG NF +LV VKTKVDP +FFR+EQSIP L
Sbjct: 508 ARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Bhi08G000828 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-148
Identity = 264/518 (50.97%), Postives = 362/518 (69.88%), Query Frame = 0

Query: 26  ASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSSYSSVLDFSIKNLRFSKPQTPKPLLII 85
           A+PQ  ++FL+CLS++   +   +K+VY+  +  Y S+L+ +++NLRF+   TPKPL+I 
Sbjct: 28  ANPQ--ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVIT 87

Query: 86  TPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFIVVDLINLRSVKVDDENN 145
           TP +VSHIQ  ++CSK  GLQIRTRSGGHD EG+SY++  PF++VDL N+ SVK+D  + 
Sbjct: 88  TPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQ 147

Query: 146 TAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHFSGGGYGLMLRKFGLAADNVI 205
           TAWVESGATLGE+YY I E +  L+FPAG CPTVG GGHFSGGGYG ++R +GLAADN+I
Sbjct: 148 TAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNII 207

Query: 206 DAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKIKLVPVPATVTICSTQRNLE 265
           DAHLV+ +GKV DR+SMGEDLFWAIRGGGG +FGI+ AWKI+LV VP+  TI S ++N+E
Sbjct: 208 DAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNME 267

Query: 266 -EDAIKLVHRWQYVANKLDENLYLGI-ILTGGKASTQG-GITNPTATFFSLFLGRIDELL 325
             + +KLV++WQ +A   ++ L L    +T      QG   T   + F S+F G +D L+
Sbjct: 268 IHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLV 327

Query: 326 ATLSTTFPELGLIKQDCVEATWVESTLIIATGVQPIESL----EPLLNRTPPTIENTKIK 385
             ++ +FPELG+ K DC + +W++ T+I  +GV    +     E LL+R+        IK
Sbjct: 328 DLMNKSFPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIK 387

Query: 386 SDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQISESETPFSHRAGNLYKIG 445
            DYVK+P+PE A+  I ++L  +D+     +F PYGG M +ISES  PF HRAG  Y+I 
Sbjct: 388 LDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIW 447

Query: 446 YVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYANYRDLDIGANNKYGQTSVEQ 505
           Y+  W++Q  +  ++HI+WIR VY +  P+VS++PR AY NYRDLD+G  N     +  Q
Sbjct: 448 YIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQ 507

Query: 506 ASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 537
           A +WG KYFG NF +LV VKTKVDP +FFR+EQSIP L
Sbjct: 508 ARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Bhi08G000828 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.7e-147
Identity = 261/538 (48.51%), Postives = 380/538 (70.63%), Query Frame = 0

Query: 1   MKFSSPLIPLALVLIVLYSCPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSSY 60
           M+FSS L    ++L  L   PL  +A    ++ FL+CLS   +++    KV+++  +SS+
Sbjct: 1   MEFSSFL--FTILLFSLNISPL-VSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSF 60

Query: 61  SSVLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120
            S+LD SI+N RFS  +TPKP+ IITP   S +Q  + C++ HG+ +RTRS GH +EGLS
Sbjct: 61  FSILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLS 120

Query: 121 YVAY-RPFIVVDLINLRSVKVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180
           Y+AY +PF V+DL NLRS+ +D +N T WV++GAT GELYY IG+ +++LAFPAG+ PTV
Sbjct: 121 YIAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTV 180

Query: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFG 240
           G+GG FSGGGYG +LRK+GLAADN+IDA +VDA+G++ DR++MGED FWAIRGGGG SFG
Sbjct: 181 GVGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFG 240

Query: 241 IVVAWKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKAST 300
           ++++WK+KLV VP+T+T+   Q+  +++A++++ +WQY A+K+ ++L++   L   + S 
Sbjct: 241 VILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTL---ERSN 300

Query: 301 QGGITNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGVQPIE 360
           +  +    A F  L++G ++ LLA +   FPELGL K+ C E +W+ES L  A   +  E
Sbjct: 301 KNAV---HALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKG-E 360

Query: 361 SLEPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQ 420
           SL  L NR   ++ + K K D+V+EP+PEAAI+ I +RL+A +    ++I  P+GGKMS+
Sbjct: 361 SLGVLTNRERTSL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSE 420

Query: 421 ISESETPFSHRAGNLYKIGYVVGWKEQSLKAK---KRHIHWIREVYEYMIPFVSKSPRAA 480
           ++E ETPF HR GNLY+I YV  W+E+  K K    +++ W+  VYE+M P+VSKSPR A
Sbjct: 421 MAEYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGA 480

Query: 481 YANYRDLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIP 535
           Y N++D+D+G      +T  E+   WG+KYF NNF++LV VKT+VDP DFF  EQSIP
Sbjct: 481 YVNFKDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of Bhi08G000828 vs. ExPASy TrEMBL
Match: A0A5A7TU57 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00190 PE=3 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 1.4e-274
Identity = 470/536 (87.69%), Postives = 501/536 (93.47%), Query Frame = 0

Query: 1   MKFSSPLIPLALVLIVLYSCPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSSY 60
           MKFSS LIPL+LV IV+ S  L A+ASPQKYQ+FLQCLSEHSS SYPISKVVY+PINSSY
Sbjct: 1   MKFSSTLIPLSLVFIVVSSASLCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSSY 60

Query: 61  SSVLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120
           SSVLDFSI+NL FSKP+TPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS
Sbjct: 61  SSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120

Query: 121 YVAYRPFIVVDLINLRSVKVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVG 180
           YVAYRPFIVVDLINLRSVKV+ ENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVG
Sbjct: 121 YVAYRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVG 180

Query: 181 IGGHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFGI 240
           IGGHFSGGGYGLMLRKFGLAADNVIDA+LVDANGKV D+ESMGEDLFWAIRGGGGGSFGI
Sbjct: 181 IGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGI 240

Query: 241 VVAWKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKASTQ 300
           VVAWKIKLVPVP TVTICST RNLEEDAIKL+HRWQYV NKLDEN+YLGIILTGG ASTQ
Sbjct: 241 VVAWKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNASTQ 300

Query: 301 GGITNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGVQPIES 360
            GITNPTA FFSLFLGR DEL+ TLST FPELGLIKQDCVEA+WVESTLII  GVQPIE+
Sbjct: 301 AGITNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIET 360

Query: 361 LEPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQI 420
           LEPLLNRT  T+++TKIKSDY+ EP+P AAI+ I QRLK QDIETSQL+FVPYGG+MSQI
Sbjct: 361 LEPLLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQI 420

Query: 421 SESETPFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYANY 480
           SESETPFSHRA  L+KIGYVVGWK+QSLKAKKRHI W+REVYEYM PFVSKSPRAAY NY
Sbjct: 421 SESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTNY 480

Query: 481 RDLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 537
           RDLDIG+NNKYG+TS ++ASVWGLKYFGNNF +LVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 RDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Bhi08G000828 vs. ExPASy TrEMBL
Match: A0A1S3BDA8 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488637 PE=3 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 1.4e-274
Identity = 470/536 (87.69%), Postives = 501/536 (93.47%), Query Frame = 0

Query: 1   MKFSSPLIPLALVLIVLYSCPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSSY 60
           MKFSS LIPL+LV IV+ S  L A+ASPQKYQ+FLQCLSEHSS SYPISKVVY+PINSSY
Sbjct: 1   MKFSSTLIPLSLVFIVVSSASLCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSSY 60

Query: 61  SSVLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120
           SSVLDFSI+NL FSKP+TPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS
Sbjct: 61  SSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120

Query: 121 YVAYRPFIVVDLINLRSVKVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVG 180
           YVAYRPFIVVDLINLRSVKV+ ENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVG
Sbjct: 121 YVAYRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVG 180

Query: 181 IGGHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFGI 240
           IGGHFSGGGYGLMLRKFGLAADNVIDA+LVDANGKV D+ESMGEDLFWAIRGGGGGSFGI
Sbjct: 181 IGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGI 240

Query: 241 VVAWKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKASTQ 300
           VVAWKIKLVPVP TVTICST RNLEEDAIKL+HRWQYV NKLDEN+YLGIILTGG ASTQ
Sbjct: 241 VVAWKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNASTQ 300

Query: 301 GGITNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGVQPIES 360
            GITNPTA FFSLFLGR DEL+ TLST FPELGLIKQDCVEA+WVESTLII  GVQPIE+
Sbjct: 301 AGITNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIET 360

Query: 361 LEPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQI 420
           LEPLLNRT  T+++TKIKSDY+ EP+P AAI+ I QRLK QDIETSQL+FVPYGG+MSQI
Sbjct: 361 LEPLLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQI 420

Query: 421 SESETPFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYANY 480
           SESETPFSHRA  L+KIGYVVGWK+QSLKAKKRHI W+REVYEYM PFVSKSPRAAY NY
Sbjct: 421 SESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTNY 480

Query: 481 RDLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 537
           RDLDIG+NNKYG+TS ++ASVWGLKYFGNNF +LVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 RDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Bhi08G000828 vs. ExPASy TrEMBL
Match: A0A5D3C1A1 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00110 PE=3 SV=1)

HSP 1 Score: 916.0 bits (2366), Expect = 7.3e-263
Identity = 443/537 (82.50%), Postives = 488/537 (90.88%), Query Frame = 0

Query: 1   MKFSSPLIPLALVLIVLYSCPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSSY 60
           MK+SSPLIP +L+LI++ S   W +ASP K+Q+FLQCLSEHSS  YPISKVV++PINSSY
Sbjct: 1   MKYSSPLIPFSLILILVSSSSSWVSASPHKHQNFLQCLSEHSSKRYPISKVVHTPINSSY 60

Query: 61  SSVLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120
           SSVLDFSI+NL FSKP+TPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS
Sbjct: 61  SSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120

Query: 121 YVAYRPFIVVDLINLRSVKVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVG 180
           YVAY PFI+VDLINLRS+K+D E NTAWVESGATLGELYYRIGEKSRTLAFP GVCPTVG
Sbjct: 121 YVAYHPFIIVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTVG 180

Query: 181 IGGHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFGI 240
           +GGHFSGGGYGLMLRKFGLAADNVIDA+LVDANGKVHDR+SMGEDLFWAIRGGGGGSFGI
Sbjct: 181 VGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFGI 240

Query: 241 VVAWKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGK-AST 300
           VVAWKIKLV VPATVTICST RNL+ D IKLVH+WQYVANKLDENL+LGI+LTGG   +T
Sbjct: 241 VVAWKIKLVSVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNITTT 300

Query: 301 QGGITNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGVQPIE 360
           Q GI NP ATFFSLFLG++D L+ATLS  FPELG  K+DCVE +W+ESTLI ATGV  +E
Sbjct: 301 QEGIANPIATFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVGTVE 360

Query: 361 SLEPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQ 420
           SLEPLLNRTP  +EN K+KSDY KEP+  A IEG+ QRLKA+DIETSQLIF+PYGG+MSQ
Sbjct: 361 SLEPLLNRTPSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRMSQ 420

Query: 421 ISESETPFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYAN 480
           ISE ETPFSHRAG LYKIGY V WKEQSLKAKK+HI WIR++Y+YM PFVSKSPR+AYAN
Sbjct: 421 ISEMETPFSHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAYAN 480

Query: 481 YRDLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 537
           YRDLDIGAN KYG+TSV QAS+WGLKYFGNNFK+LVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 YRDLDIGANKKYGKTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of Bhi08G000828 vs. ExPASy TrEMBL
Match: A0A1S3BDZ5 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488631 PE=3 SV=1)

HSP 1 Score: 916.0 bits (2366), Expect = 7.3e-263
Identity = 443/537 (82.50%), Postives = 488/537 (90.88%), Query Frame = 0

Query: 1   MKFSSPLIPLALVLIVLYSCPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSSY 60
           MK+SSPLIP +L+LI++ S   W +ASP K+Q+FLQCLSEHSS  YPISKVV++PINSSY
Sbjct: 1   MKYSSPLIPFSLILILVSSSSSWVSASPHKHQNFLQCLSEHSSKRYPISKVVHTPINSSY 60

Query: 61  SSVLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120
           SSVLDFSI+NL FSKP+TPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS
Sbjct: 61  SSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120

Query: 121 YVAYRPFIVVDLINLRSVKVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVG 180
           YVAY PFI+VDLINLRS+K+D E NTAWVESGATLGELYYRIGEKSRTLAFP GVCPTVG
Sbjct: 121 YVAYHPFIIVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTVG 180

Query: 181 IGGHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFGI 240
           +GGHFSGGGYGLMLRKFGLAADNVIDA+LVDANGKVHDR+SMGEDLFWAIRGGGGGSFGI
Sbjct: 181 VGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFGI 240

Query: 241 VVAWKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGK-AST 300
           VVAWKIKLV VPATVTICST RNL+ D IKLVH+WQYVANKLDENL+LGI+LTGG   +T
Sbjct: 241 VVAWKIKLVSVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNITTT 300

Query: 301 QGGITNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGVQPIE 360
           Q GI NP ATFFSLFLG++D L+ATLS  FPELG  K+DCVE +W+ESTLI ATGV  +E
Sbjct: 301 QEGIANPIATFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVGTVE 360

Query: 361 SLEPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQ 420
           SLEPLLNRTP  +EN K+KSDY KEP+  A IEG+ QRLKA+DIETSQLIF+PYGG+MSQ
Sbjct: 361 SLEPLLNRTPSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRMSQ 420

Query: 421 ISESETPFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYAN 480
           ISE ETPFSHRAG LYKIGY V WKEQSLKAKK+HI WIR++Y+YM PFVSKSPR+AYAN
Sbjct: 421 ISEMETPFSHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAYAN 480

Query: 481 YRDLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 537
           YRDLDIGAN KYG+TSV QAS+WGLKYFGNNFK+LVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 YRDLDIGANKKYGKTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of Bhi08G000828 vs. ExPASy TrEMBL
Match: A0A1S3BDB7 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488652 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 8.4e-259
Identity = 431/539 (79.96%), Postives = 489/539 (90.72%), Query Frame = 0

Query: 1   MKFSSPLIPL--ALVLIVLYSCPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINS 60
           MK SS L PL   L+++V+ S PLW +A P ++Q+FL CL EHSS SYPISKVVY+PINS
Sbjct: 1   MKSSSQLTPLFHVLIIVVVSSSPLWVSAFPHEHQNFLHCLPEHSSKSYPISKVVYTPINS 60

Query: 61  SYSSVLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEG 120
           SYSSVL+FSI+N RFSKP+TPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHD+EG
Sbjct: 61  SYSSVLNFSIRNFRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG 120

Query: 121 LSYVAYRPFIVVDLINLRSVKVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPT 180
           LSYVAYRPFI+VDLIN+RS+ VD  NNTAWVESGATLGELYYRIGEKSRTLAFPAG+CPT
Sbjct: 121 LSYVAYRPFIIVDLINIRSISVDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT 180

Query: 181 VGIGGHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSF 240
           VG+GGHFSGGGYG +LRK+GLAADNVIDA+LVDANG  HDRESMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMAHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKAS 300
           GIVVAWK+KLVPVPA VTICST +NLE DA+K++H+WQYVANKL E+++LGI+LTGG  S
Sbjct: 241 GIVVAWKVKLVPVPALVTICSTIKNLEGDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300

Query: 301 TQGGITNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGV-QP 360
           TQGGI  PTATF+SLFLG++DEL+ATLSTTFPE GLIK+DC+E  W+ESTLIIA+GV Q 
Sbjct: 301 TQGGIKKPTATFYSLFLGKVDELVATLSTTFPEFGLIKKDCLEVRWIESTLIIASGVFQT 360

Query: 361 IESLEPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKM 420
           +ESL+PLLNRTP TI++TKIKSDY+K+P+P+AAIEGI QRLKA DIE  QL+FVPYG +M
Sbjct: 361 VESLKPLLNRTPSTIDSTKIKSDYIKKPIPKAAIEGIWQRLKAGDIEAPQLVFVPYGARM 420

Query: 421 SQISESETPFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAY 480
           SQISESETPFSHRAG LYKIGY+VGWK Q+LKAK RHI W RE+YEYM PFVSKSPR AY
Sbjct: 421 SQISESETPFSHRAGYLYKIGYMVGWKGQNLKAKNRHISWTRELYEYMTPFVSKSPRTAY 480

Query: 481 ANYRDLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 537
            NYRDLDIG+NNKYG+TS +QAS+WGLKYFGNNFK+LVYVK KVDP+DFFRHEQSIPTL
Sbjct: 481 VNYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQSIPTL 539

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G20820.15.2e-15250.47FAD-binding Berberine family protein [more]
AT5G44440.11.2e-14848.51FAD-binding Berberine family protein [more]
AT1G30700.12.9e-14748.87FAD-binding Berberine family protein [more]
AT5G44400.15.6e-14647.89FAD-binding Berberine family protein [more]
AT2G34790.13.6e-14548.11FAD-binding Berberine family protein [more]
Match NameE-valueIdentityDescription
Q9SVG57.3e-15150.47Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=... [more]
Q8GTB63.1e-14950.98Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV... [more]
A6P6W08.9e-14950.97Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=... [more]
A6P6W11.5e-14850.97Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=... [more]
Q9FI211.7e-14748.51Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=... [more]
Match NameE-valueIdentityDescription
A0A5A7TU571.4e-27487.69Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BDA81.4e-27487.69tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10348863... [more]
A0A5D3C1A17.3e-26382.50Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BDZ57.3e-26382.50tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10348863... [more]
A0A1S3BDB78.4e-25979.96tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10348865... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.462.20coord: 254..472
e-value: 1.6E-160
score: 536.6
NoneNo IPR availablePANTHERPTHR32448:SF142CANNABIDIOLIC ACID SYNTHASE-LIKEcoord: 5..536
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 5..536
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..20
score: 5.0
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 29..135
e-value: 2.7E-44
score: 151.4
IPR012951Berberine/berberine-likePFAMPF08031BBEcoord: 476..534
e-value: 6.9E-19
score: 67.7
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 81..217
e-value: 2.8E-28
score: 98.4
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 139..534
e-value: 1.6E-160
score: 536.6
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 77..251
score: 20.134073
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 23..252

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M000828Bhi08M000828mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0071949 FAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding