Bhi08G000771 (gene) Wax gourd (B227) v1

Overview
NameBhi08G000771
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionEukaryotic aspartyl protease family protein
Locationchr8: 29900864 .. 29902396 (-)
RNA-Seq ExpressionBhi08G000771
SyntenyBhi08G000771
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCAATATCAACCTCCTTTGCAAAAAAAATCTTGAGCTATTTCCTTCTTCTTGTATATGTCTCAAGAAAAACCCTAGCTACAAACCCTAAAACCAATGGCCCCAAAGATTCTCTAGTTATTGGTCTTGTTCATTCAAGAACAACCCTCCTTACCCCAAAAAAAGGCTATAATTTCATTTCAAGGAAGAGAATGAAGGCAATGGAAATGGATGATAATGTGATAGAGCCATTGAGGGAAATTAGGGATGGTTATTTGATGTCCTTAACATTAGGGACACCCCCACAAGTTATTCAAGTCTATATGGACACAGGAAGTGATCTGACATGGGTTCCTTGTGGCAACCTCTCTTTTGATTGTCAAGATTGTGAGGAGTATCAAAACAATGTTTCAGGCCCAAAATTGGCTGCTTTTTTGCCTACTCATTCTTCTACTTCTATTAGAGACACTTGTGGTAGCTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCCTTGCTACCCTTGTGAAGGCCACTTGCCCTAGACCTTGCCCTTCATTTGCTTATACTTATGGAGCAAGTGGGGTTGTAATTGGAACTTTAACAAGAGATGTCCTTTTAATGCATATAAATAATATTAATTCTCCAAATTCTTCCACTAAGAAAACCCCTAGGTTTTGTTTTGGATGTGTTGGTGCAAGTTATAGAGAGCCAATTGGGATTGCTGGCTTTGGTAGAGGGTTACTTTCTCTTCCTTTTCAGTTAGGGTTTTCTCATAAGGGGTTTTCTCATTGCTTCTTGCCTTTTAAATTCTCAAATAACCCTAATTTTTCAAGCCCTTTGATTCTTGGTAACCTTGCTGTTTCTTCAAAAGATGAGCATTTGCAATTTACTCCTTTGTTGAAAAGTCCAATTTACCCCAACTACTACTATATTGGGCTTGAGTCAATCACTATAGGAAATGGGAATAGTAATTTTAGATTTGGAGTTTCTTTCAACTTGAGAGAGATTGATACAAAGGGTAATGGGGGAATGTTGATTGATTCTGGTACTACTTATACTCATTTACCTGAACCATTGTATTCACAACTTATTTCAAATCTTGAGTCAGTGATAAGCTATCCAAGAGCTAAACAAGTTGAACTCAATACTGGATTTGATCTTTGTTATAAAGTTCCTTGTAAAAACAACAATTTTTCTTTTATTGATGACTCTCAACTCCCTTCTATAACATTCCATTTTTTGAATAATGTTAGTGTTGTTTTGCCCCAAGAGAACAATTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGCTTGTTGTTTCAAAGCATGGACGGTGTGGGTGGCGATACCGACGACGACCGAGATGGGCCGGCGGGTATTTTCGGAAGCTTCCAACAACAAAATTTGGAGGTTGTTTATGACTTGGAGAAGGAAAGATTAGGGTTTCAACCAATGGATTGTGCTTATGTTGCTGCCACTCAAGGACTACACAAGAATGTTTGA

mRNA sequence

ATGGCTTCAATATCAACCTCCTTTGCAAAAAAAATCTTGAGCTATTTCCTTCTTCTTGTATATGTCTCAAGAAAAACCCTAGCTACAAACCCTAAAACCAATGGCCCCAAAGATTCTCTAGTTATTGGTCTTGTTCATTCAAGAACAACCCTCCTTACCCCAAAAAAAGGCTATAATTTCATTTCAAGGAAGAGAATGAAGGCAATGGAAATGGATGATAATGTGATAGAGCCATTGAGGGAAATTAGGGATGGTTATTTGATGTCCTTAACATTAGGGACACCCCCACAAGTTATTCAAGTCTATATGGACACAGGAAGTGATCTGACATGGGTTCCTTGTGGCAACCTCTCTTTTGATTGTCAAGATTGTGAGGAGTATCAAAACAATGTTTCAGGCCCAAAATTGGCTGCTTTTTTGCCTACTCATTCTTCTACTTCTATTAGAGACACTTGTGGTAGCTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCCTTGCTACCCTTGTGAAGGCCACTTGCCCTAGACCTTGCCCTTCATTTGCTTATACTTATGGAGCAAGTGGGGTTGTAATTGGAACTTTAACAAGAGATGTCCTTTTAATGCATATAAATAATATTAATTCTCCAAATTCTTCCACTAAGAAAACCCCTAGGTTTTGTTTTGGATGTGTTGGTGCAAGTTATAGAGAGCCAATTGGGATTGCTGGCTTTGGTAGAGGGTTACTTTCTCTTCCTTTTCAGTTAGGGTTTTCTCATAAGGGGTTTTCTCATTGCTTCTTGCCTTTTAAATTCTCAAATAACCCTAATTTTTCAAGCCCTTTGATTCTTGGTAACCTTGCTGTTTCTTCAAAAGATGAGCATTTGCAATTTACTCCTTTGTTGAAAAGTCCAATTTACCCCAACTACTACTATATTGGGCTTGAGTCAATCACTATAGGAAATGGGAATAGTAATTTTAGATTTGGAGTTTCTTTCAACTTGAGAGAGATTGATACAAAGGGTAATGGGGGAATGTTGATTGATTCTGGTACTACTTATACTCATTTACCTGAACCATTGTATTCACAACTTATTTCAAATCTTGAGTCAGTGATAAGCTATCCAAGAGCTAAACAAGTTGAACTCAATACTGGATTTGATCTTTGTTATAAAGTTCCTTGTAAAAACAACAATTTTTCTTTTATTGATGACTCTCAACTCCCTTCTATAACATTCCATTTTTTGAATAATGTTAGTGTTGTTTTGCCCCAAGAGAACAATTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGCTTGTTGTTTCAAAGCATGGACGGTGTGGGTGGCGATACCGACGACGACCGAGATGGGCCGGCGGGTATTTTCGGAAGCTTCCAACAACAAAATTTGGAGGTTGTTTATGACTTGGAGAAGGAAAGATTAGGGTTTCAACCAATGGATTGTGCTTATGTTGCTGCCACTCAAGGACTACACAAGAATGTTTGA

Coding sequence (CDS)

ATGGCTTCAATATCAACCTCCTTTGCAAAAAAAATCTTGAGCTATTTCCTTCTTCTTGTATATGTCTCAAGAAAAACCCTAGCTACAAACCCTAAAACCAATGGCCCCAAAGATTCTCTAGTTATTGGTCTTGTTCATTCAAGAACAACCCTCCTTACCCCAAAAAAAGGCTATAATTTCATTTCAAGGAAGAGAATGAAGGCAATGGAAATGGATGATAATGTGATAGAGCCATTGAGGGAAATTAGGGATGGTTATTTGATGTCCTTAACATTAGGGACACCCCCACAAGTTATTCAAGTCTATATGGACACAGGAAGTGATCTGACATGGGTTCCTTGTGGCAACCTCTCTTTTGATTGTCAAGATTGTGAGGAGTATCAAAACAATGTTTCAGGCCCAAAATTGGCTGCTTTTTTGCCTACTCATTCTTCTACTTCTATTAGAGACACTTGTGGTAGCTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCCTTGCTACCCTTGTGAAGGCCACTTGCCCTAGACCTTGCCCTTCATTTGCTTATACTTATGGAGCAAGTGGGGTTGTAATTGGAACTTTAACAAGAGATGTCCTTTTAATGCATATAAATAATATTAATTCTCCAAATTCTTCCACTAAGAAAACCCCTAGGTTTTGTTTTGGATGTGTTGGTGCAAGTTATAGAGAGCCAATTGGGATTGCTGGCTTTGGTAGAGGGTTACTTTCTCTTCCTTTTCAGTTAGGGTTTTCTCATAAGGGGTTTTCTCATTGCTTCTTGCCTTTTAAATTCTCAAATAACCCTAATTTTTCAAGCCCTTTGATTCTTGGTAACCTTGCTGTTTCTTCAAAAGATGAGCATTTGCAATTTACTCCTTTGTTGAAAAGTCCAATTTACCCCAACTACTACTATATTGGGCTTGAGTCAATCACTATAGGAAATGGGAATAGTAATTTTAGATTTGGAGTTTCTTTCAACTTGAGAGAGATTGATACAAAGGGTAATGGGGGAATGTTGATTGATTCTGGTACTACTTATACTCATTTACCTGAACCATTGTATTCACAACTTATTTCAAATCTTGAGTCAGTGATAAGCTATCCAAGAGCTAAACAAGTTGAACTCAATACTGGATTTGATCTTTGTTATAAAGTTCCTTGTAAAAACAACAATTTTTCTTTTATTGATGACTCTCAACTCCCTTCTATAACATTCCATTTTTTGAATAATGTTAGTGTTGTTTTGCCCCAAGAGAACAATTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGCTTGTTGTTTCAAAGCATGGACGGTGTGGGTGGCGATACCGACGACGACCGAGATGGGCCGGCGGGTATTTTCGGAAGCTTCCAACAACAAAATTTGGAGGTTGTTTATGACTTGGAGAAGGAAAGATTAGGGTTTCAACCAATGGATTGTGCTTATGTTGCTGCCACTCAAGGACTACACAAGAATGTTTGA

Protein sequence

MASISTSFAKKILSYFLLLVYVSRKTLATNPKTNGPKDSLVIGLVHSRTTLLTPKKGYNFISRKRMKAMEMDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDEHLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFHFLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQNLEVVYDLEKERLGFQPMDCAYVAATQGLHKNV
Homology
BLAST of Bhi08G000771 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 587.0 bits (1512), Expect = 1.4e-167
Identity = 294/498 (59.04%), Postives = 367/498 (73.69%), Query Frame = 0

Query: 18  LLVYVSRKTLATNPKTNGPKDS--LVIGLVHSRTTLLTPKKGYNFISRKRMKAMEMDDNV 77
           LL+  + KT A   K      S  LV+ L  S  +L TPK   +    +  K +   D V
Sbjct: 16  LLLNTTNKTQARQHKNPSSSSSSFLVLTLTKSSVSLPTPK---SQTQERIKKPLSSVDVV 75

Query: 78  IEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNN-VSGP 137
           +EPLRE+RDGYL++L +GTPPQ +QVY+DTGSDLTWVPCGNLSFDC +C + +NN +  P
Sbjct: 76  MEPLREVRDGYLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSFDCIECYDLKNNDLKSP 135

Query: 138 KLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKATCPRPCPSFAY 197
             + F P HSSTS RD+C SSFC++IHSSDNPFDPC +AGCS++ L+K+TC RPCPSFAY
Sbjct: 136 --SVFSPLHSSTSFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSMLLKSTCVRPCPSFAY 195

Query: 198 TYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGASYREPIGIAGFGRGLL 257
           TYG  G++ G LTRD+L           + T+  PRF FGCV ++YREPIGIAGFGRGLL
Sbjct: 196 TYGEGGLISGILTRDIL----------KARTRDVPRFSFGCVTSTYREPIGIAGFGRGLL 255

Query: 258 SLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVS-SKDEHLQFTPLLKSPIYPN 317
           SLP QLGF  KGFSHCFLPFKF NNPN SSPLILG  A+S +  + LQFTP+L +P+YPN
Sbjct: 256 SLPSQLGFLEKGFSHCFLPFKFVNNPNISSPLILGASALSINLTDSLQFTPMLNTPMYPN 315

Query: 318 YYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNL 377
            YYIGLESITIG   +  +  V   LR+ D++GNGGML+DSGTTYTHLPEP YSQL++ L
Sbjct: 316 SYYIGLESITIGTNITPTQ--VPLTLRQFDSQGNGGMLVDSGTTYTHLPEPFYSQLLTTL 375

Query: 378 ESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQL---PSITFHFLNNVSVVLPQE 437
           +S I+YPRA + E  TGFDLCYKVPC NNN + +++  +   PSITFHFLNN +++LPQ 
Sbjct: 376 QSTITYPRATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFPSITFHFLNNATLLLPQG 435

Query: 438 NNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQNLEVVYDLEKERL 497
           N+FYAM+AP + +VV+CLLFQ+M       +D   GPAG+FGSFQQQN++VVYDLEKER+
Sbjct: 436 NSFYAMSAPSDGSVVQCLLFQNM-------EDGDYGPAGVFGSFQQQNVKVVYDLEKERI 489

Query: 498 GFQPMDCAYVAATQGLHK 509
           GFQ MDC   AA+ GL++
Sbjct: 496 GFQAMDCVLEAASHGLNQ 489

BLAST of Bhi08G000771 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 214.9 bits (546), Expect = 1.5e-55
Identity = 150/446 (33.63%), Postives = 212/446 (47.53%), Query Frame = 0

Query: 86  YLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVSGPKLAAFLPTHSS 145
           YL+SL++G+    + +Y+DTGSDL W PC    F C  CE      S P       + SS
Sbjct: 83  YLISLSVGSSSSAVSLYLDTGSDLVWFPC--RPFTCILCESKPLPPSPPS------SLSS 142

Query: 146 TSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKATC---PRPCPSFAYTYGASGVV 205
           ++   +C S  C   HSS    D C I+ C L  +    C     PCP F Y YG   + 
Sbjct: 143 SATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSL- 202

Query: 206 IGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGASYREPIGIAGFGRGLLSLPFQLGF 265
                  V  ++ ++++ P+ S      F FGC   +  EPIG+AGFGRG LSLP QL  
Sbjct: 203 -------VAKLYSDSLSLPSVSVS---NFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAV 262

Query: 266 --SHKG--FSHCFLPFKF-SNNPNFSSPLILGNLA------VSSKDEH------------ 325
              H G  FS+C +   F S+     SPLILG         V + D+H            
Sbjct: 263 HSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNE 322

Query: 326 LQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTYT 385
             FT +L++P +P +Y + L+ I+IG  N          LR ID  G GG+++DSGTT+T
Sbjct: 323 FVFTEMLENPKHPYFYSVSLQGISIGKRN----IPAPAMLRRIDKNGGGGVVVDSGTTFT 382

Query: 386 HLPEPLYSQLISNLESVIS--YPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITF 445
            LP   Y+ ++   +S +   + RA +VE ++G   CY +             ++P++  
Sbjct: 383 MLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN---------QTVKVPALVL 442

Query: 446 HFL-NNVSVVLPQENNFYAMA----APINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFG 499
           HF  N  SV LP+ N FY              + CL+  +    GGD  + R G   I G
Sbjct: 443 HFAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMN----GGDESELRGGTGAILG 492

BLAST of Bhi08G000771 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 174.9 bits (442), Expect = 1.7e-43
Identity = 132/423 (31.21%), Postives = 198/423 (46.81%), Query Frame = 0

Query: 85  GYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVSGPKLAAFLPTHS 144
           GY +SL+ GTP Q I    DTGS L W+PC +  + C  C+   + +    +  F+P +S
Sbjct: 89  GYSVSLSFGTPSQTIPFVFDTGSSLVWLPCTS-RYLCSGCD--FSGLDPTLIPRFIPKNS 148

Query: 145 STSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKATCPRPCPSFAYTYGASGVVIG 204
           S+S    C S  C  ++    P   C   GC   T     C   CP +   YG  G   G
Sbjct: 149 SSSKIIGCQSPKCQFLY---GPNVQC--RGCDPNT---RNCTVGCPPYILQYGL-GSTAG 208

Query: 205 TLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGASYREPIGIAGFGRGLLSLPFQLGFSH 264
            L  +        ++ P+ +    P F  GC   S R+P GIAGFGRG +SLP Q+    
Sbjct: 209 VLITE-------KLDFPDLT---VPDFVVGCSIISTRQPAGIAGFGRGPVSLPSQMNL-- 268

Query: 265 KGFSHCFLPFKFSNNPNFSSPLILGNLA---VSSKDEHLQFTPLLKSPIYPN-----YYY 324
           K FSHC +  +F ++ N ++ L L   +     SK   L +TP  K+P   N     YYY
Sbjct: 269 KRFSHCLVSRRF-DDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVSNKAFLEYYY 328

Query: 325 IGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLESV 384
           + L  I +G  +      + +      T G+GG ++DSG+T+T +  P++  +     S 
Sbjct: 329 LNLRRIYVGRKHVK----IPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEEFASQ 388

Query: 385 IS-YPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFHFLNNVSVVLPQENNFYA 444
           +S Y R K +E  TG   C+ +  K        D  +P + F F     + LP  +N++ 
Sbjct: 389 MSNYTREKDLEKETGLGPCFNISGKG-------DVTVPELIFEFKGGAKLELPL-SNYFT 448

Query: 445 MAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQNLEVVYDLEKERLGFQPM 499
                ++  +  +  ++++  GG       GPA I GSFQQQN  V YDLE +R GF   
Sbjct: 449 FVGNTDTVCLTVVSDKTVNPSGG------TGPAIILGSFQQQNYLVEYDLENDRFGFAKK 468

BLAST of Bhi08G000771 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 159.8 bits (403), Expect = 5.6e-39
Identity = 128/451 (28.38%), Postives = 187/451 (41.46%), Query Frame = 0

Query: 59  NFISRKRMKAMEMDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLS 118
           +F+S +R     +   V+         Y + L +G PPQ + +  DTGSDL WV C    
Sbjct: 57  HFLSLRRKPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCS--- 116

Query: 119 FDCQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLA 178
             C++C  +           F P HSST     C    C  +   D        A     
Sbjct: 117 -ACRNCSHHS------PATVFFPRHSSTFSPAHCYDPVCRLVPKPDR-------APICNH 176

Query: 179 TLVKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGC--- 238
           T + +TC        Y YG +    G+LT  +      ++ + +    +     FGC   
Sbjct: 177 TRIHSTC-------HYEYGYAD---GSLTSGLFARETTSLKTSSGKEARLKSVAFGCGFR 236

Query: 239 ------VGASYREPIGIAGFGRGLLSLPFQLG--FSHKGFSHCFLPFKFSNNPNFSSPLI 298
                  G S+    G+ G GRG +S   QLG  F +K FS+C + +  S  P  +S LI
Sbjct: 237 ISGQSVSGTSFNGANGVMGLGRGPISFASQLGRRFGNK-FSYCLMDYTLSPPP--TSYLI 296

Query: 299 LGNLAVSSKDEHLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGN 358
           +GN         L FTPLL +P+ P +YY+ L+S+ +       +  +  ++ EID  GN
Sbjct: 297 IGN--GGDGISKLFFTPLLTNPLSPTFYYVKLKSVFVNGA----KLRIDPSIWEIDDSGN 356

Query: 359 GGMLIDSGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFI 418
           GG ++DSGTT   L EP Y  +I+ +   +  P A    L  GFDLC  V          
Sbjct: 357 GGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIAD--ALTPGFDLCVNVSGVTK----- 416

Query: 419 DDSQLPSITFHFLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGP 478
            +  LP + F F      V P  N F           ++CL  QS+D   G         
Sbjct: 417 PEKILPRLKFEFSGGAVFVPPPRNYFIE-----TEEQIQCLAIQSVDPKVG--------- 450

Query: 479 AGIFGSFQQQNLEVVYDLEKERLGFQPMDCA 499
             + G+  QQ     +D ++ RLGF    CA
Sbjct: 477 FSVIGNLMQQGFLFEFDRDRSRLGFSRRGCA 450

BLAST of Bhi08G000771 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 149.8 bits (377), Expect = 5.8e-36
Identity = 156/529 (29.49%), Postives = 222/529 (41.97%), Query Frame = 0

Query: 5   STSFAKKILSYFLLL------VYVSRKTL--ATNPKTNGPKDSLVIGLVH-----SRTTL 64
           S+S +  +  +FL+L      V  SR++L   T PK N P+    + L H     + T +
Sbjct: 3   SSSSSSLLFPFFLILFSCLISVSSSRRSLIDRTLPK-NLPRSGFRLSLRHVDSGKNLTKI 62

Query: 65  LTPKKGYN--FISRKRMKAMEM-------DD--NVIEPLREIRDGYLMSLTLGTPPQVIQ 124
              ++G N  F    R+ A+ +       DD  N+  P       +LM L++G P     
Sbjct: 63  QKIQRGINRGFHRLNRLGAVAVLAVASKPDDTNNIKAPTHGGSGEFLMELSIGNPAVKYS 122

Query: 125 VYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDI 184
             +DTGSDL W  C      C +C +    +       F P  SS+  +  C S  C  +
Sbjct: 123 AIVDTGSDLIWTQCK----PCTECFDQPTPI-------FDPEKSSSYSKVGCSSGLCNAL 182

Query: 185 HSSDNPFDPCTIAGCSLATLVKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINS 244
             S+   D             K  C      + YTYG      G L  +       N  S
Sbjct: 183 PRSNCNED-------------KDAC-----EYLYTYGDYSSTRGLLATETFTFEDENSIS 242

Query: 245 PNSSTKKTPRFCFGC----VGASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKF 304
                       FGC     G  + +  G+ G GRG LSL  QL      FS+C    + 
Sbjct: 243 ---------GIGFGCGVENEGDGFSQGSGLVGLGRGPLSLISQL--KETKFSYCLTSIED 302

Query: 305 SNNPNFSSPLILGNLA--------VSSKDEHLQFTPLLKSPIYPNYYYIGLESITIGNGN 364
           S     SS L +G+LA         S   E  +   LL++P  P++YY+ L+ IT+G   
Sbjct: 303 S---EASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQPSFYYLELQGITVGAK- 362

Query: 365 SNFRFGVSFNLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLESVISYPRAKQVELN 424
              R  V  +  E+   G GGM+IDSGTT T+L E  +  L     S +S P       +
Sbjct: 363 ---RLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLP--VDDSGS 422

Query: 425 TGFDLCYKVPCKNNNFSFIDDSQLPSITFHFLNNVSVVLPQENNFYAMAAPINSTVVKCL 484
           TG DLC+K+P    N +      +P + FHF     + LP EN   A     +ST V CL
Sbjct: 423 TGLDLCFKLPDAAKNIA------VPKMIFHF-KGADLELPGENYMVA----DSSTGVLCL 458

Query: 485 LFQSMDGVGGDTDDDRDGPAGIFGSFQQQNLEVVYDLEKERLGFQPMDC 498
              S +G+             IFG+ QQQN  V++DLEKE + F P +C
Sbjct: 483 AMGSSNGM------------SIFGNVQQQNFNVLHDLEKETVSFVPTEC 458

BLAST of Bhi08G000771 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 2.1e-54
Identity = 150/446 (33.63%), Postives = 212/446 (47.53%), Query Frame = 0

Query: 86  YLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVSGPKLAAFLPTHSS 145
           YL+SL++G+    + +Y+DTGSDL W PC    F C  CE      S P       + SS
Sbjct: 83  YLISLSVGSSSSAVSLYLDTGSDLVWFPC--RPFTCILCESKPLPPSPPS------SLSS 142

Query: 146 TSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKATC---PRPCPSFAYTYGASGVV 205
           ++   +C S  C   HSS    D C I+ C L  +    C     PCP F Y YG   + 
Sbjct: 143 SATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSL- 202

Query: 206 IGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGASYREPIGIAGFGRGLLSLPFQLGF 265
                  V  ++ ++++ P+ S      F FGC   +  EPIG+AGFGRG LSLP QL  
Sbjct: 203 -------VAKLYSDSLSLPSVSVS---NFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAV 262

Query: 266 --SHKG--FSHCFLPFKF-SNNPNFSSPLILGNLA------VSSKDEH------------ 325
              H G  FS+C +   F S+     SPLILG         V + D+H            
Sbjct: 263 HSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNE 322

Query: 326 LQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTYT 385
             FT +L++P +P +Y + L+ I+IG  N          LR ID  G GG+++DSGTT+T
Sbjct: 323 FVFTEMLENPKHPYFYSVSLQGISIGKRN----IPAPAMLRRIDKNGGGGVVVDSGTTFT 382

Query: 386 HLPEPLYSQLISNLESVIS--YPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITF 445
            LP   Y+ ++   +S +   + RA +VE ++G   CY +             ++P++  
Sbjct: 383 MLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN---------QTVKVPALVL 442

Query: 446 HFL-NNVSVVLPQENNFYAMA----APINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFG 499
           HF  N  SV LP+ N FY              + CL+  +    GGD  + R G   I G
Sbjct: 443 HFAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMN----GGDESELRGGTGAILG 492

BLAST of Bhi08G000771 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 6.9e-34
Identity = 125/439 (28.47%), Postives = 177/439 (40.32%), Query Frame = 0

Query: 64  KRMKAMEMDDNVIEPLREIRDG-YLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQ 123
           + + AM    + IE      DG YLM++ +GTP       MDTGSDL W  C      C 
Sbjct: 73  RSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCE----PCT 132

Query: 124 DCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVK 183
            C      +       F P  SS+     C S +C D+     P + C    C       
Sbjct: 133 QCFSQPTPI-------FNPQDSSSFSTLPCESQYCQDL-----PSETCNNNEC------- 192

Query: 184 ATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGC----VGA 243
                    + Y YG      G +  +               T   P   FGC     G 
Sbjct: 193 --------QYTYGYGDGSTTQGYMATETFTF----------ETSSVPNIAFGCGEDNQGF 252

Query: 244 SYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDE 303
                 G+ G G G LSLP QLG     FS+C   +  S+     S L LG+ A S   E
Sbjct: 253 GQGNGAGLIGMGWGPLSLPSQLGVGQ--FSYCMTSYGSSS----PSTLALGS-AASGVPE 312

Query: 304 HLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTY 363
               T L+ S + P YYYI L+ IT+G  N     G+  +  ++   G GGM+IDSGTT 
Sbjct: 313 GSPSTTLIHSSLNPTYYYITLQGITVGGDN----LGIPSSTFQLQDDGTGGMIIDSGTTL 372

Query: 364 THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFH 423
           T+LP+  Y+ +       I+ P     E ++G   C++ P   +        Q+P I+  
Sbjct: 373 TYLPQDAYNAVAQAFTDQINLPTVD--ESSSGLSTCFQQPSDGSTV------QVPEISMQ 432

Query: 424 FLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQN 483
           F   V + L ++N    + +P    +  CL   S   +G            IFG+ QQQ 
Sbjct: 433 FDGGV-LNLGEQN---ILISPAEGVI--CLAMGSSSQLG----------ISIFGNIQQQE 435

Query: 484 LEVVYDLEKERLGFQPMDC 498
            +V+YDL+   + F P  C
Sbjct: 493 TQVLYDLQNLAVSFVPTQC 435

BLAST of Bhi08G000771 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 7.7e-33
Identity = 124/428 (28.97%), Postives = 185/428 (43.22%), Query Frame = 0

Query: 74  NVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVSG 133
           +V+  L +    Y   L +GTP + + + +DTGSD+ W+ C      C+ C    + +  
Sbjct: 130 SVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCA----PCRRCYSQSDPI-- 189

Query: 134 PKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKATCPRPCPSFA 193
                F P  S T     C S  C  + S          AGC+     + TC      + 
Sbjct: 190 -----FDPRKSKTYATIPCSSPHCRRLDS----------AGCNTR---RKTC-----LYQ 249

Query: 194 YTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGASYREPIGIAGFGRGL 253
            +YG     +G  + + L    N +              F  VGA+     G+ G G+G 
Sbjct: 250 VSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLF--VGAA-----GLLGLGKGK 309

Query: 254 LSLPFQLG--FSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDEHLQFTPLLKSPIY 313
           LS P Q G  F+ K FS+C +    S+ P   S ++ GN AVS      +FTPLL +P  
Sbjct: 310 LSFPGQTGHRFNQK-FSYCLVDRSASSKP---SSVVFGNAAVS---RIARFTPLLSNPKL 369

Query: 314 PNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLIS 373
             +YY+GL  I++G        GV+ +L ++D  GNGG++IDSGT+ T L  P Y  +  
Sbjct: 370 DTFYYVGLLGISVGGTRVP---GVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRD 429

Query: 374 NLE-SVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFHFLNNVSVVLPQE 433
                  +  RA    L   FD C+       + S +++ ++P++  HF     V LP  
Sbjct: 430 AFRVGAKTLKRAPDFSL---FDTCF-------DLSNMNEVKVPTVVLHF-RGADVSLPAT 485

Query: 434 NNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQNLEVVYDLEKERL 493
           N       P+++    C  F     +GG           I G+ QQQ   VVYDL   R+
Sbjct: 490 N----YLIPVDTNGKFCFAFAGT--MGG---------LSIIGNIQQQGFRVVYDLASSRV 485

Query: 494 GFQPMDCA 499
           GF P  CA
Sbjct: 550 GFAPGGCA 485

BLAST of Bhi08G000771 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 4.2e-31
Identity = 129/443 (29.12%), Postives = 181/443 (40.86%), Query Frame = 0

Query: 64  KRMKAMEMDDNVIEPLREIRDG-YLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQ 123
           +R++AM    + +E      DG YLM+L++GTP Q     MDTGSDL W         CQ
Sbjct: 72  QRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWT-------QCQ 131

Query: 124 DCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVK 183
            C +  N         F P  SS+     C S  C  + S                    
Sbjct: 132 PCTQCFNQ----STPIFNPQGSSSFSTLPCSSQLCQALSS-------------------- 191

Query: 184 ATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGC----VGA 243
            TC      + Y YG      G++  + L             +   P   FGC     G 
Sbjct: 192 PTCSNNFCQYTYGYGDGSETQGSMGTETLTF----------GSVSIPNITFGCGENNQGF 251

Query: 244 SYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDE 303
                 G+ G GRG LSLP QL  +   FS+C  P   S   N    L+LG+LA +S   
Sbjct: 252 GQGNGAGLVGMGRGPLSLPSQLDVTK--FSYCMTPIGSSTPSN----LLLGSLA-NSVTA 311

Query: 304 HLQFTPLLKSPIYPNYYYIGLESITIGNGN---SNFRFGVSFNLREIDTKGNGGMLIDSG 363
               T L++S   P +YYI L  +++G+         F ++ N       G GG++IDSG
Sbjct: 312 GSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSN------NGTGGIIIDSG 371

Query: 364 TTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSI 423
           TT T+     Y  +     S I+ P       ++GFDLC++ P   +N       Q+P+ 
Sbjct: 372 TTLTYFVNNAYQSVRQEFISQINLPVVN--GSSSGFDLCFQTPSDPSNL------QIPTF 431

Query: 424 TFHFLNNVSVVLPQENNFYAMAAPINSTVVKCL-LFQSMDGVGGDTDDDRDGPAGIFGSF 483
             HF +   + LP EN F    +P N  +  CL +  S  G+             IFG+ 
Sbjct: 432 VMHF-DGGDLELPSENYF---ISPSNGLI--CLAMGSSSQGM------------SIFGNI 434

Query: 484 QQQNLEVVYDLEKERLGFQPMDC 498
           QQQN+ VVYD     + F    C
Sbjct: 492 QQQNMLVVYDTGNSVVSFASAQC 434

BLAST of Bhi08G000771 vs. ExPASy Swiss-Prot
Match: O04496 (Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 3.3e-28
Identity = 113/426 (26.53%), Postives = 182/426 (42.72%), Query Frame = 0

Query: 86  YLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVSGPKLAAFLPTHSS 145
           Y++   LGTPPQ++ + +DT +D  W+PC      C  C                 +++S
Sbjct: 104 YVVRAKLGTPPQLMFMVLDTSNDAVWLPCSG----CSGC-----------------SNAS 163

Query: 146 TSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKATCPRPCP-----SFAYTYGASG 205
           TS      S++             C+ A C+ A     TCP   P     SF  +YG   
Sbjct: 164 TSFNTNSSSTYST---------VSCSTAQCTQAR--GLTCPSSSPQPSVCSFNQSYGGDS 223

Query: 206 VVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGASYRE---PIGIAGFGRGLLSLP 265
               +L +D L +  + I          P F FGC+ ++      P G+ G GRG +SL 
Sbjct: 224 SFSASLVQDTLTLAPDVI----------PNFSFGCINSASGNSLPPQGLMGLGRGPMSLV 283

Query: 266 FQLGFSHKG-FSHCFLPFKFSNNPNFSSPLILGNLAVSSKDEHLQFTPLLKSPIYPNYYY 325
            Q    + G FS+C   F+   +  FS  L LG L    + + +++TPLL++P  P+ YY
Sbjct: 284 SQTTSLYSGVFSYCLPSFR---SFYFSGSLKLGLL---GQPKSIRYTPLLRNPRRPSLYY 343

Query: 326 IGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLESV 385
           + L  +++G    + +  V       D     G +IDSGT  T   +P+Y  +       
Sbjct: 344 VNLTGVSVG----SVQVPVDPVYLTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFR-- 403

Query: 386 ISYPRAKQVELNT-----GFDLCYKVPCKNNNFSFIDDSQLPSITFHFLNNVSVVLPQEN 445
                 KQV +++      FD C         FS  +++  P IT H + ++ + LP EN
Sbjct: 404 ------KQVNVSSFSTLGAFDTC---------FSADNENVAPKITLH-MTSLDLKLPMEN 448

Query: 446 NFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQNLEVVYDLEKERLG 498
                +A      + CL   SM G+      + +    +  + QQQNL +++D+   R+G
Sbjct: 464 TLIHSSA----GTLTCL---SMAGI----RQNANAVLNVIANLQQQNLRILFDVPNSRIG 448

BLAST of Bhi08G000771 vs. ExPASy TrEMBL
Match: A0A5A7TNC9 (Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G00970 PE=3 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.5e-268
Identity = 453/512 (88.48%), Postives = 479/512 (93.55%), Query Frame = 0

Query: 5   STSFAKKILSYFLLLVYVSRKTLATNPKTNGPKDSLVIGLVHSRTTLLTPKKGYNFISRK 64
           STS A K LS FLLLV+ S++TLATNPKTN PKDSLV+GLVHSRT+LLTPKKGYNFIS+K
Sbjct: 6   STSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYNFISKK 65

Query: 65  RMKAMEM---DDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDC 124
           RMKAM+    DDNVIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGNLSFDC
Sbjct: 66  RMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDC 125

Query: 125 QDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLV 184
           QDCEEYQNN+SGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLV
Sbjct: 126 QDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLV 185

Query: 185 KATCPRPCPSFAYTYGASGVVIGTLTRDVLLMH---INNINSPNSSTKKTPRFCFGCVGA 244
           K TCPRPCPSFAYTYGASGVV G+LTRDVL MH    NN N+ +++ K+ PRFCFGCVGA
Sbjct: 186 KGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCFGCVGA 245

Query: 245 SYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDE 304
           +YREPIGIAGFGRGLLSLPFQLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LA+SSKDE
Sbjct: 246 TYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAISSKDE 305

Query: 305 HLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTY 364
           +LQFTPLLKSPIYPNYYYIGLESITIGNGN+NFRFGVSF LREIDTKGNGGMLIDSGTTY
Sbjct: 306 NLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTY 365

Query: 365 THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFH 424
           THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNN SF+DDSQLPSITFH
Sbjct: 366 THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPSITFH 425

Query: 425 FLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQN 484
           FLNNVSVVLPQ NNFYAMAAPINSTVVKCLL+QSMDGVG D D D +GPAGIFGSFQQQN
Sbjct: 426 FLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQN 485

Query: 485 LEVVYDLEKERLGFQPMDCAYVAATQGLHKNV 511
           L+VVYDLEKERLGFQ MDC  VAA QGLHKNV
Sbjct: 486 LQVVYDLEKERLGFQAMDCVSVAANQGLHKNV 517

BLAST of Bhi08G000771 vs. ExPASy TrEMBL
Match: A0A1S3CAK9 (aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103498305 PE=3 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.5e-268
Identity = 453/512 (88.48%), Postives = 479/512 (93.55%), Query Frame = 0

Query: 5   STSFAKKILSYFLLLVYVSRKTLATNPKTNGPKDSLVIGLVHSRTTLLTPKKGYNFISRK 64
           STS A K LS FLLLV+ S++TLATNPKTN PKDSLV+GLVHSRT+LLTPKKGYNFIS+K
Sbjct: 6   STSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYNFISKK 65

Query: 65  RMKAMEM---DDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDC 124
           RMKAM+    DDNVIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGNLSFDC
Sbjct: 66  RMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDC 125

Query: 125 QDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLV 184
           QDCEEYQNN+SGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLV
Sbjct: 126 QDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLV 185

Query: 185 KATCPRPCPSFAYTYGASGVVIGTLTRDVLLMH---INNINSPNSSTKKTPRFCFGCVGA 244
           K TCPRPCPSFAYTYGASGVV G+LTRDVL MH    NN N+ +++ K+ PRFCFGCVGA
Sbjct: 186 KGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCFGCVGA 245

Query: 245 SYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDE 304
           +YREPIGIAGFGRGLLSLPFQLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LA+SSKDE
Sbjct: 246 TYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAISSKDE 305

Query: 305 HLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTY 364
           +LQFTPLLKSPIYPNYYYIGLESITIGNGN+NFRFGVSF LREIDTKGNGGMLIDSGTTY
Sbjct: 306 NLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTY 365

Query: 365 THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFH 424
           THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNN SF+DDSQLPSITFH
Sbjct: 366 THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPSITFH 425

Query: 425 FLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQN 484
           FLNNVSVVLPQ NNFYAMAAPINSTVVKCLL+QSMDGVG D D D +GPAGIFGSFQQQN
Sbjct: 426 FLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQN 485

Query: 485 LEVVYDLEKERLGFQPMDCAYVAATQGLHKNV 511
           L+VVYDLEKERLGFQ MDC  VAA QGLHKNV
Sbjct: 486 LQVVYDLEKERLGFQAMDCVSVAANQGLHKNV 517

BLAST of Bhi08G000771 vs. ExPASy TrEMBL
Match: A0A0A0LYP0 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G704590 PE=3 SV=1)

HSP 1 Score: 929.5 bits (2401), Expect = 6.1e-267
Identity = 451/514 (87.74%), Postives = 480/514 (93.39%), Query Frame = 0

Query: 1   MASIST-SFAKKILSYFLLLVYVSRKTLATNPKTNGPKDSLVIGLVHSRTTLLTPKKGYN 60
           M SIS+ S A K LS FLLLV+VS +TLATNPKTN PKDSLV+GLVHSRT+LLTPKKGYN
Sbjct: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISRKRMKAMEM---DDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120
           FIS+KRMKAM+    DDNVIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNN+SGP+LAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCV 240
           LA+LVK TCPRPCPSFAYTYGASGVV G+LTRDVL  H  N N+ N++ K+ PRFCFGCV
Sbjct: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTH-GNYNNNNNNNKQIPRFCFGCV 240

Query: 241 GASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSK 300
           GA+YREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLA+SSK
Sbjct: 241 GATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK 300

Query: 301 DEHLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGT 360
           DE+LQFTPLLKSP+YPNYYYIGLESITIGNG++NFRFGVSF LREIDTKGNGGMLIDSGT
Sbjct: 301 DENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGT 360

Query: 361 TYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSIT 420
           TYTHLPEPLYSQLISNLE VI YPRAKQVELNTGFDLCYKVPCKNNN SF+DD+QLPSIT
Sbjct: 361 TYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSIT 420

Query: 421 FHFLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQ 480
           FHFLNNVSVVLPQ NNFYAMAAPINSTVVKCLL+QSMDGVG D D D +GPAGIFGSFQQ
Sbjct: 421 FHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQ 480

Query: 481 QNLEVVYDLEKERLGFQPMDCAYVAATQGLHKNV 511
           QN+EVVYDLEKERLGFQPMDC  VAA QGLHKNV
Sbjct: 481 QNIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNV 513

BLAST of Bhi08G000771 vs. ExPASy TrEMBL
Match: A0A6J1EHM1 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111434252 PE=3 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 5.6e-236
Identity = 417/512 (81.45%), Postives = 450/512 (87.89%), Query Frame = 0

Query: 1   MASISTSFAKKILSYFLLLVYVSRKTLA---TNPKTNGPKDSLVIGLVHSRTTLLTPKKG 60
           MASI+   A+      L+LV VS + +     NPKT   KDSLV+GLVHSRT+LLTPK+G
Sbjct: 1   MASIA---ARSYFVLVLVLVLVSGEAMGQTLANPKTKFLKDSLVLGLVHSRTSLLTPKRG 60

Query: 61  YNFISRKRMKAMEM-DDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120
           YN +  KR+K MEM +D+VIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN
Sbjct: 61  YNSLLTKRIKPMEMGNDDVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDC+EYQNNV GPKLAAFLPTHSSTSIRDTCGSSFC+DIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCDEYQNNVLGPKLAAFLPTHSSTSIRDTCGSSFCIDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCV 240
           LATLVK TCPRPCPSF+YTYGASG+VIGTLT+DV+ +H    NSPNSS +K P+FCFGCV
Sbjct: 181 LATLVKGTCPRPCPSFSYTYGASGLVIGTLTKDVIFIH---GNSPNSS-RKIPKFCFGCV 240

Query: 241 GASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSK 300
           GA+YREPIGIAGFGRGLLSLP QLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLA+SSK
Sbjct: 241 GATYREPIGIAGFGRGLLSLPSQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK 300

Query: 301 DEHLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGT 360
            EHL+FTP LKSP YPNYYYIGLESITIGNG +  RFGVS  LREIDTKGNGG+LIDSGT
Sbjct: 301 -EHLKFTPFLKSPFYPNYYYIGLESITIGNGENYSRFGVSLQLREIDTKGNGGILIDSGT 360

Query: 361 TYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSIT 420
           TYTHLPEPLYSQLISNLES+ISYPRAK+ ELNTGFDLCYKVP KNN F F D+ +LPSIT
Sbjct: 361 TYTHLPEPLYSQLISNLESLISYPRAKEHELNTGFDLCYKVPYKNNTF-FSDEFELPSIT 420

Query: 421 FHFLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQ 480
           FHFLNNVSVVLPQ N+FYAMAAP NSTVVKCLLFQSMDG       D DGPAGIFGSFQQ
Sbjct: 421 FHFLNNVSVVLPQGNSFYAMAAPSNSTVVKCLLFQSMDG-------DGDGPAGIFGSFQQ 480

Query: 481 QNLEVVYDLEKERLGFQPMDCAYVAATQGLHK 509
           QNLEVVYDLEKERLGF+ MDCA VA +QGLHK
Sbjct: 481 QNLEVVYDLEKERLGFEAMDCASVAVSQGLHK 496

BLAST of Bhi08G000771 vs. ExPASy TrEMBL
Match: A0A6J1CMP8 (probable aspartyl protease At4g16563 OS=Momordica charantia OX=3673 GN=LOC111012684 PE=3 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 1.6e-235
Identity = 423/526 (80.42%), Postives = 458/526 (87.07%), Query Frame = 0

Query: 3   SISTSFAKKILSYFLLLVYV--SRKTLATNPKTNGPK-DSLVIGLVHSRTTLLTPKKGYN 62
           S +T+ + K+L++FLLL+ +    +T A   + N P  DSLV+GLVHSRT+LLTPK+GY 
Sbjct: 6   SSATTISSKVLTFFLLLILLLSVSETAARPHRNNFPNTDSLVLGLVHSRTSLLTPKRGY- 65

Query: 63  FISRKRM-----KAMEM--DDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWV 122
             SRK       K ME    DNVIEPLREIRDGYL+SLTLGTPPQVIQVYMDTGSDLTWV
Sbjct: 66  -FSRKGSSSSINKPMEEIGSDNVIEPLREIRDGYLISLTLGTPPQVIQVYMDTGSDLTWV 125

Query: 123 PCGNLSFDCQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTI 182
           PCGNLSFDCQDCEEYQNNVSGPKLA F PTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTI
Sbjct: 126 PCGNLSFDCQDCEEYQNNVSGPKLAPFSPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTI 185

Query: 183 AGCSLATLVKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFC 242
           AGCSLATLVK TCPRPCPSFAYTYGASGVV GTLT+DV+LMH     SPNS+T + PRFC
Sbjct: 186 AGCSLATLVKGTCPRPCPSFAYTYGASGVVTGTLTKDVILMH---GVSPNSTT-QIPRFC 245

Query: 243 FGCVGASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLA 302
           FGCVGA+YREPIGIAGFGRGLLSLP QLGFSHKGFSHCFLPFKFSNNPNFSSPLILG+LA
Sbjct: 246 FGCVGATYREPIGIAGFGRGLLSLPSQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGSLA 305

Query: 303 VSSKDEHLQFTPLLKSPIYPNYYYIGLESITIGN--GNSNFRFGVSFNLREIDTKGNGGM 362
           +SSKD  LQFTPLLKSP+YPNYYYIGLES+TIG+  GN+N RFGVS  LREIDTKGNGGM
Sbjct: 306 ISSKDHSLQFTPLLKSPLYPNYYYIGLESVTIGDGIGNNNSRFGVSLKLREIDTKGNGGM 365

Query: 363 LIDSGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNN-NF-SFID 422
           LIDSGTTYTHLPEPLYSQLISNLESV++YPRAKQVE+NTGFDLCYK+PCKNN NF S +D
Sbjct: 366 LIDSGTTYTHLPEPLYSQLISNLESVVTYPRAKQVEINTGFDLCYKIPCKNNTNFSSSMD 425

Query: 423 D---SQLPSITFHFLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTD--DD 482
           D   + LPSITFHFLNNVSVVLPQ NNFYAMAAP NSTVVKCLLFQSMDG GGD D   D
Sbjct: 426 DYCSNLLPSITFHFLNNVSVVLPQGNNFYAMAAPTNSTVVKCLLFQSMDGGGGDGDGNGD 485

Query: 483 RDGPAGIFGSFQQQNLEVVYDLEKERLGFQPMDCAYVAATQGLHKN 510
            DGPAGIFGSFQQQN+EVVYDL+KER+GFQ MDCA  AA+QGLHKN
Sbjct: 486 DDGPAGIFGSFQQQNMEVVYDLQKERIGFQTMDCASSAASQGLHKN 525

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G45120.11.4e-16759.04Eukaryotic aspartyl protease family protein [more]
AT4G16563.11.5e-5533.63Eukaryotic aspartyl protease family protein [more]
AT3G52500.11.7e-4331.21Eukaryotic aspartyl protease family protein [more]
AT3G25700.15.6e-3928.38Eukaryotic aspartyl protease family protein [more]
AT2G03200.15.8e-3629.49Eukaryotic aspartyl protease family protein [more]
Match NameE-valueIdentityDescription
Q940R42.1e-5433.63Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656... [more]
Q766C26.9e-3428.47Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q9LNJ37.7e-3328.97Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q766C34.2e-3129.12Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
O044963.3e-2826.53Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7TNC91.5e-26888.48Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3CAK91.5e-26888.48aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103498305 PE=3 S... [more]
A0A0A0LYP06.1e-26787.74Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G70459... [more]
A0A6J1EHM15.6e-23681.45probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114342... [more]
A0A6J1CMP81.6e-23580.42probable aspartyl protease At4g16563 OS=Momordica charantia OX=3673 GN=LOC111012... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 290..503
e-value: 1.8E-46
score: 160.1
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 80..289
e-value: 1.0E-28
score: 102.6
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 85..498
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 86..290
e-value: 3.6E-27
score: 95.7
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 315..493
e-value: 4.4E-27
score: 94.8
NoneNo IPR availablePANTHERPTHR47967:SF47CHLOROPLAST NUCLEOID DNA-BINDING PROTEIN-LIKEcoord: 49..502
NoneNo IPR availablePANTHERPTHR47967OS07G0603500 PROTEIN-RELATEDcoord: 49..502
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 350..361
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 86..493
score: 33.526558
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 85..497
e-value: 1.17245E-72
score: 229.842

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M000771Bhi08M000771mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005576 extracellular region
molecular_function GO:0004190 aspartic-type endopeptidase activity