Bhi08G000585 (gene) Wax gourd (B227) v1

Overview
NameBhi08G000585
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionsubtilisin-like protease SBT5.4
Locationchr8: 23032854 .. 23036998 (-)
RNA-Seq ExpressionBhi08G000585
SyntenyBhi08G000585
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATTTCAGTCTTATGTTGTGTTATTGGGATCTCATTCACATGGGATAGAAATTACAGAGGAAGATCTTGAAAGGGTGGTTCATTCCCATCATAAATTGCTTGGATCCTTCTTGGGAAGGTTTGAAAATTTTATCTAGTTTCCTATATTTCATTTAATTGTTTTTTTTTTTCAAATATTGTTATAAATATAAATTTTAAAATTGAAAATTATTTCAGTGAAGAGAAGGCAAAAGATGCCATATTTTACTCCTATAAGAAGCATATAAACGGTTTTGCAGCAACTCTTGATGAGGAAGAGGCAACCAAACTTGCAGGTAAAAACCAAACCATATATACTTTTCTTTTAAATTGTATTTTCATATTTTTTTGGCATGACGAATATTGAGTAATTAAAATAGAATTATAATAATAAAAAGAATTAATTGGGAAATTATAAAAATGGCACAGAACACCCAGAAGTAGCAGCCATATTGGCAAATAGAGCAAAAGCATTACACACAACACATTCATGGGAGTTTATGCATTTGGAGAAGAATGGTGTAATTCCCTCTTCCTCTCCTTGGCGTATAGCTAAATCTGGAAAAGATGTCATTATTGCAAATCTTGACACTGGTAAGTTTTTTCTGTTTTCTTTTCTTTTCTTTATTGGGTAAATTAACATAAATTGAACAAATATAAATATGAAATTCGATGGAATTAAAATTTTGTTTGAAAAACCCAATGCGATCAACCCCAACTTATAGTATTTGTATTTTTTTTTGCTATTACATTTTTTCTATTTGTAAAAATTTCCCTTTAAAATATATATTTTTGTTACTTATAATATAATTACTTATAATTTTAGTTTTCTTTAATTTCATTACATTTTTAATAATTTATTCTCAATCTTATGAAAAGAAAATTTTCAATTACATAAATTGAAATTGAATGAATTGTCTTAATTCAATATATAAAAATAATTAAATTAGAATATTTACATTTCACTTCTTTTACTTCTTTTTACTTTTTTTTAGAACGAATAACCTAAACCAACCCAACTCCAATACCTTCATGGTTTGGTAGGATTAGGTTTATTTTTTAATAAGAATTATTTTGTTAAAAAATTTACAACTCGAACAATTAAATTATGTCTAAAATATTCTTCAATCCAATACACGAACACTCCTAAAGTTTAGGGTTGAAATGAATGTTATTATTTTGTTTTATTTCTATTTGAAAAAGGTGTATGGCCGGAGTCCAAGAGTTTTGGAGAACATGGCATAGTTGGACCTGTCCCATCAAAGTGGAAGGGAGGCTGCATGGATGAAACCCCTGATCGAGTGCCATGCAACAGGTTTGATTATCAGTTTGATTTTTTTAATATTGAGATTAAGGTTGTTTAGTTAATTTAACAATGATTTAAAAATAAAATGCAGGAAATTAATCGGAGCAAAGTACTTCAACAAGGGCTTTATTGCATACTTAAAATCTGAAAATTCCACAGAATTCTCATCAGTCATTAACTCCACCCGTGACTACGAAGGTCATGGCTCCCACACCTTGTCCACGGCCGGGGGCAGCTATGTGTCCGGCGCCAGTGTGTTCGGTTCAGGCGCTGGAACTGCCAAAGGGGGCTCTCCCAAGGCCCGTGTTGCCGCTTATAAGGTCTGCTGGCCACTCGAGGGTGGAGGTTGTTTCGATTCCGATATTGTCGAGGCATTCGACCATGCCATTCACGACGGTGTTGATGTTCTTTCTCTCTCCCTTGGTAGTGATCCAGCCGAATACTCTGAAGATTCCATTGCCATCGCAGCCTTCCATGCAGTGAAGAAAGGAATCCCCGTCGTATGCTCGGCCGGCAACTCCGGCCCCGATGCTCTGACTGCTTCTAATACTGCACCTTGGATTTTGACGGTTGGTGCCAGTACTTTGGACCGTGAGTTTCAGGCTCCTGTTGAGCTCCAAAATGGCCATCGTTACATGGTTTGTCTCTCTCTCTCTCTCTCTAATTACCAGCTTTATCTTTATATTTTGCAAATTAATCTAACATAACACGCATTTTTTCCCTTTTGGGATGCTTGTAGCTTTAAAGATTTCAAATAAATTTGTATTATTGATTGAATGATCACGACTTGTGGGATTTTAAATACAGAATTTAAAGTTAAATTAGTAAATTTTATTTATCTACAAATAAATGAGAAGTAAAAGCTTATATATTGTACCAATAAACATTTTATATGTTCTTGTTCTTTTTCGGTTATCACTCAATACATTCATTCCAAATTTTCCTTGTTACACATAAAATAAAAATTGAAAAATCAATGAAAGTCATTTCAAAGTAAAAAATATTTATGCCCATTTTTGTGCGGTTTTTTTAATAATAATTTTTTTATATTGATTGAAAGGAATGTATGGAGAGGTAATACCTAAGTATTACTATTTAAAATCACTGAAACTTCATCATTAGTCTCTTTTCCAGAGTTTCCAAAGACTAAATTTGTAATCGAACTAAAAATATCTCATTTAAGCTTAGTATCAAACATAGAATGAATTTGACCTAGGTATTAGGCATGCATGAAATTTTTGGTACTAGAATTTTTTTTTGTTTTTTTTTTTTTTGGAAGAAATGAATGTGTGTGGGTTATATTTTCAAAGTAAATAAAAGAGTAGTATTTAACATTATTTGTTGGTACTGATTAGGGTTCGAGTCTTTCAAAAGCATTACAAGGAAATAAGTTGTACCCATTGATAACCGGAGCTCAGGCAAAAGCGAAGAACGCCGCCATGGACGACGCCATGCTTTGCAAGCCGGAAACATTGGATCATTCAAAAGTGAAAGGGAAAATATTGGCTTGCTTGAGAGGAGACAATGCAAGAGTGGATAAAGGAGAACAAGCTGCCCTTGCCGGTGCTGTCGGAATGATTCTATGCAACGATGAACTCAGTGGGTTCGAAACCATCGCCGATCCCCATGTTCTTCCAGCTTCCCATATTAATTACAATGACGGTCAAGCTGTTCTCTCCTACATCAATTCCACCAAGTAAATCAAATTTTACACTCTTTCTATTTTTTTCTTTTATTTTTGAAAATTTGGTAATTGATTAATTAATTAATGGTGAGAATTTTTGTTTTTTTTTTGGTAGAAATCCAATGGGGTATTTAATCCCACCGTCGGCTAAAGTTAACACCAAACCTGCTCCTACCATGGCGGCCTTCTCATCTCGAGGACCCAATGTTATTTCCCCTGAGATTATCAAGGTTTTCTTTTTCTCTTCCTTTATTTTTAAATTTTCTCTTAAACATATATTTTATACTTTTATCTAAATATCATAAATAAAAAAAAATCTAAAATATTATATTAATATTGATATTATTCTAACTTTTTTGGATCAAATTTTGCAGCCGGATGTGACGGCACCAGGCGTGAATGTTATCGCAGCATTCTCAGAAGCCGTAAGCCCAACAGGTGAGGCATTCGACAACAGAACAGTTCCATTCATAACAATGTCGGGCACCTCCATGTCTTGCCCCCACGTCTCCGGTCTAGTCGGCCTCCTTAGAACTCTCCACCCCCACTGGAGCCCCTCTGCCATCAAATCCGCCATCATGACCTCTGCGAGAATTCGCGACAACACAATGAAACCCATGCTCGATGGCGGCTCCCCTGACCTTGCCCCTGCCACCCCCTTTGCTTATGGCTCTGGCCACATCCGCCCCACCGGAGCCATTGACCCTGGCCTCGTCTATGACCTTTCCCCCAACGATTACCTAGAATTCCTCTGTGCCAGTGGCTACGACGACAAAACCATTCGAGCCTTCTCTGATGGCCCTTTCAAGTGTCCACCCAATGCTTCTATTTTGAACTTCAATTATCCTTCCATTGGGGTTCAGAATTTGAATGGCAATGTCACCGTCACTAGAAAACTGAAGAACGTTAGCACTCCAGGGGTTTACAGAGCCAGAATTATGAACCCAGATGGAGTTAAGGTTTCGGTGAAGCCCAAAGTTTTGAAGTTTGAGAGAGTTGCAGAGGAGAAAAGCTTTGAATTGACGATGACTGGAAATGTGCCAGAAGATCAGGTTGTGGACGGCGTGCTGATTTGGACTGATGGCAAGCACTTTGTTAGGAGTCCCATTGTCATTTCTTCTGTCTTGTTTTGA

mRNA sequence

ATGGGATTTCAGTCTTATGTTGTGTTATTGGGATCTCATTCACATGGGATAGAAATTACAGAGGAAGATCTTGAAAGGGTGGTTCATTCCCATCATAAATTGCTTGGATCCTTCTTGGGAAGTGAAGAGAAGGCAAAAGATGCCATATTTTACTCCTATAAGAAGCATATAAACGGTTTTGCAGCAACTCTTGATGAGGAAGAGGCAACCAAACTTGCAGAACACCCAGAAGTAGCAGCCATATTGGCAAATAGAGCAAAAGCATTACACACAACACATTCATGGGAGTTTATGCATTTGGAGAAGAATGGTGTAATTCCCTCTTCCTCTCCTTGGCGTATAGCTAAATCTGGAAAAGATGTCATTATTGCAAATCTTGACACTGGTGTATGGCCGGAGTCCAAGAGTTTTGGAGAACATGGCATAGTTGGACCTGTCCCATCAAAGTGGAAGGGAGGCTGCATGGATGAAACCCCTGATCGAGTGCCATGCAACAGGAAATTAATCGGAGCAAAGTACTTCAACAAGGGCTTTATTGCATACTTAAAATCTGAAAATTCCACAGAATTCTCATCAGTCATTAACTCCACCCGTGACTACGAAGGTCATGGCTCCCACACCTTGTCCACGGCCGGGGGCAGCTATGTGTCCGGCGCCAGTGTGTTCGGTTCAGGCGCTGGAACTGCCAAAGGGGGCTCTCCCAAGGCCCGTGTTGCCGCTTATAAGGTCTGCTGGCCACTCGAGGGTGGAGGTTGTTTCGATTCCGATATTGTCGAGGCATTCGACCATGCCATTCACGACGGTGTTGATGTTCTTTCTCTCTCCCTTGGTAGTGATCCAGCCGAATACTCTGAAGATTCCATTGCCATCGCAGCCTTCCATGCAGTGAAGAAAGGAATCCCCGTCGTATGCTCGGCCGGCAACTCCGGCCCCGATGCTCTGACTGCTTCTAATACTGCACCTTGGATTTTGACGGTTGGTGCCAGTACTTTGGACCGTGAGTTTCAGGCTCCTGTTGAGCTCCAAAATGGCCATCGTTACATGGGTTCGAGTCTTTCAAAAGCATTACAAGGAAATAAGTTGTACCCATTGATAACCGGAGCTCAGGCAAAAGCGAAGAACGCCGCCATGGACGACGCCATGCTTTGCAAGCCGGAAACATTGGATCATTCAAAAGTGAAAGGGAAAATATTGGCTTGCTTGAGAGGAGACAATGCAAGAGTGGATAAAGGAGAACAAGCTGCCCTTGCCGGTGCTGTCGGAATGATTCTATGCAACGATGAACTCAGTGGGTTCGAAACCATCGCCGATCCCCATGTTCTTCCAGCTTCCCATATTAATTACAATGACGGTCAAGCTGTTCTCTCCTACATCAATTCCACCAAAAATCCAATGGGGTATTTAATCCCACCGTCGGCTAAAGTTAACACCAAACCTGCTCCTACCATGGCGGCCTTCTCATCTCGAGGACCCAATGTTATTTCCCCTGAGATTATCAAGCCGGATGTGACGGCACCAGGCGTGAATGTTATCGCAGCATTCTCAGAAGCCGTAAGCCCAACAGGTGAGGCATTCGACAACAGAACAGTTCCATTCATAACAATGTCGGGCACCTCCATGTCTTGCCCCCACGTCTCCGGTCTAGTCGGCCTCCTTAGAACTCTCCACCCCCACTGGAGCCCCTCTGCCATCAAATCCGCCATCATGACCTCTGCGAGAATTCGCGACAACACAATGAAACCCATGCTCGATGGCGGCTCCCCTGACCTTGCCCCTGCCACCCCCTTTGCTTATGGCTCTGGCCACATCCGCCCCACCGGAGCCATTGACCCTGGCCTCGTCTATGACCTTTCCCCCAACGATTACCTAGAATTCCTCTGTGCCAGTGGCTACGACGACAAAACCATTCGAGCCTTCTCTGATGGCCCTTTCAAGTGTCCACCCAATGCTTCTATTTTGAACTTCAATTATCCTTCCATTGGGGTTCAGAATTTGAATGGCAATGTCACCGTCACTAGAAAACTGAAGAACGTTAGCACTCCAGGGGTTTACAGAGCCAGAATTATGAACCCAGATGGAGTTAAGGTTTCGGTGAAGCCCAAAGTTTTGAAGTTTGAGAGAGTTGCAGAGGAGAAAAGCTTTGAATTGACGATGACTGGAAATGTGCCAGAAGATCAGGTTGTGGACGGCGTGCTGATTTGGACTGATGGCAAGCACTTTGTTAGGAGTCCCATTGTCATTTCTTCTGTCTTGTTTTGA

Coding sequence (CDS)

ATGGGATTTCAGTCTTATGTTGTGTTATTGGGATCTCATTCACATGGGATAGAAATTACAGAGGAAGATCTTGAAAGGGTGGTTCATTCCCATCATAAATTGCTTGGATCCTTCTTGGGAAGTGAAGAGAAGGCAAAAGATGCCATATTTTACTCCTATAAGAAGCATATAAACGGTTTTGCAGCAACTCTTGATGAGGAAGAGGCAACCAAACTTGCAGAACACCCAGAAGTAGCAGCCATATTGGCAAATAGAGCAAAAGCATTACACACAACACATTCATGGGAGTTTATGCATTTGGAGAAGAATGGTGTAATTCCCTCTTCCTCTCCTTGGCGTATAGCTAAATCTGGAAAAGATGTCATTATTGCAAATCTTGACACTGGTGTATGGCCGGAGTCCAAGAGTTTTGGAGAACATGGCATAGTTGGACCTGTCCCATCAAAGTGGAAGGGAGGCTGCATGGATGAAACCCCTGATCGAGTGCCATGCAACAGGAAATTAATCGGAGCAAAGTACTTCAACAAGGGCTTTATTGCATACTTAAAATCTGAAAATTCCACAGAATTCTCATCAGTCATTAACTCCACCCGTGACTACGAAGGTCATGGCTCCCACACCTTGTCCACGGCCGGGGGCAGCTATGTGTCCGGCGCCAGTGTGTTCGGTTCAGGCGCTGGAACTGCCAAAGGGGGCTCTCCCAAGGCCCGTGTTGCCGCTTATAAGGTCTGCTGGCCACTCGAGGGTGGAGGTTGTTTCGATTCCGATATTGTCGAGGCATTCGACCATGCCATTCACGACGGTGTTGATGTTCTTTCTCTCTCCCTTGGTAGTGATCCAGCCGAATACTCTGAAGATTCCATTGCCATCGCAGCCTTCCATGCAGTGAAGAAAGGAATCCCCGTCGTATGCTCGGCCGGCAACTCCGGCCCCGATGCTCTGACTGCTTCTAATACTGCACCTTGGATTTTGACGGTTGGTGCCAGTACTTTGGACCGTGAGTTTCAGGCTCCTGTTGAGCTCCAAAATGGCCATCGTTACATGGGTTCGAGTCTTTCAAAAGCATTACAAGGAAATAAGTTGTACCCATTGATAACCGGAGCTCAGGCAAAAGCGAAGAACGCCGCCATGGACGACGCCATGCTTTGCAAGCCGGAAACATTGGATCATTCAAAAGTGAAAGGGAAAATATTGGCTTGCTTGAGAGGAGACAATGCAAGAGTGGATAAAGGAGAACAAGCTGCCCTTGCCGGTGCTGTCGGAATGATTCTATGCAACGATGAACTCAGTGGGTTCGAAACCATCGCCGATCCCCATGTTCTTCCAGCTTCCCATATTAATTACAATGACGGTCAAGCTGTTCTCTCCTACATCAATTCCACCAAAAATCCAATGGGGTATTTAATCCCACCGTCGGCTAAAGTTAACACCAAACCTGCTCCTACCATGGCGGCCTTCTCATCTCGAGGACCCAATGTTATTTCCCCTGAGATTATCAAGCCGGATGTGACGGCACCAGGCGTGAATGTTATCGCAGCATTCTCAGAAGCCGTAAGCCCAACAGGTGAGGCATTCGACAACAGAACAGTTCCATTCATAACAATGTCGGGCACCTCCATGTCTTGCCCCCACGTCTCCGGTCTAGTCGGCCTCCTTAGAACTCTCCACCCCCACTGGAGCCCCTCTGCCATCAAATCCGCCATCATGACCTCTGCGAGAATTCGCGACAACACAATGAAACCCATGCTCGATGGCGGCTCCCCTGACCTTGCCCCTGCCACCCCCTTTGCTTATGGCTCTGGCCACATCCGCCCCACCGGAGCCATTGACCCTGGCCTCGTCTATGACCTTTCCCCCAACGATTACCTAGAATTCCTCTGTGCCAGTGGCTACGACGACAAAACCATTCGAGCCTTCTCTGATGGCCCTTTCAAGTGTCCACCCAATGCTTCTATTTTGAACTTCAATTATCCTTCCATTGGGGTTCAGAATTTGAATGGCAATGTCACCGTCACTAGAAAACTGAAGAACGTTAGCACTCCAGGGGTTTACAGAGCCAGAATTATGAACCCAGATGGAGTTAAGGTTTCGGTGAAGCCCAAAGTTTTGAAGTTTGAGAGAGTTGCAGAGGAGAAAAGCTTTGAATTGACGATGACTGGAAATGTGCCAGAAGATCAGGTTGTGGACGGCGTGCTGATTTGGACTGATGGCAAGCACTTTGTTAGGAGTCCCATTGTCATTTCTTCTGTCTTGTTTTGA

Protein sequence

MGFQSYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFLGSEEKAKDAIFYSYKKHINGFAATLDEEEATKLAEHPEVAAILANRAKALHTTHSWEFMHLEKNGVIPSSSPWRIAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETPDRVPCNRKLIGAKYFNKGFIAYLKSENSTEFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVFGSGAGTAKGGSPKARVAAYKVCWPLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSDPAEYSEDSIAIAAFHAVKKGIPVVCSAGNSGPDALTASNTAPWILTVGASTLDREFQAPVELQNGHRYMGSSLSKALQGNKLYPLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILACLRGDNARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVLSYINSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVSPTGEAFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTMKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAFSDGPFKCPPNASILNFNYPSIGVQNLNGNVTVTRKLKNVSTPGVYRARIMNPDGVKVSVKPKVLKFERVAEEKSFELTMTGNVPEDQVVDGVLIWTDGKHFVRSPIVISSVLF
Homology
BLAST of Bhi08G000585 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 891.0 bits (2301), Expect = 6.8e-259
Identity = 444/747 (59.44%), Postives = 553/747 (74.03%), Query Frame = 0

Query: 4   QSYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFLGSEEKAKDAIFYSYKKHINGFAAT 63
           +SY+V LGSH+H  +I+   L+ V HSH   L SF+GS E AK+AIFYSYK+HINGFAA 
Sbjct: 40  KSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAI 99

Query: 64  LDEEEATKLAEHPEVAAILANRAKALHTTHSWEFMHLEKNGVIPSSSPWRIAKSGKDVII 123
           LDE EA ++A+HP+V ++  N+ + LHTTHSW FM L KNGV+  SS W  A  G+D II
Sbjct: 100 LDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTII 159

Query: 124 ANLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETPDRVPCNRKLIGAKYFNKGFIAYLK 183
           ANLDTGVWPESKSF + G  G VP++WKG C  +    VPCNRKLIGA+YFNKG++AY  
Sbjct: 160 ANLDTGVWPESKSFSDEG-YGAVPARWKGRCHKD----VPCNRKLIGARYFNKGYLAYTG 219

Query: 184 SENSTEFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVFGSGAGTAKGGSPKARVAAYKV 243
             ++  +     + RD++GHGSHTLSTA G++V GA+VFG G GTA GGSPKARVAAYKV
Sbjct: 220 LPSNASY----ETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKV 279

Query: 244 CW-PLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSDPAEYSEDSIAIAAFHAVKKGIPV 303
           CW P++G  CFD+DI+ A + AI DGVDVLS S+G D  +Y  D IAI +FHAVK G+ V
Sbjct: 280 CWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTV 339

Query: 304 VCSAGNSGPDALTASNTAPWILTVGASTLDREFQAPVELQNGHRYMGSSLSKALQGNKLY 363
           VCSAGNSGP + T SN APW++TVGAS++DREFQA VEL+NG  + G+SLSK L   K+Y
Sbjct: 340 VCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLSKPLPEEKMY 399

Query: 364 PLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILACLRGDNARVDKGEQAALAGAVGM 423
            LI+ A A   N  + DA+LCK  +LD  KVKGKIL CLRGDNARVDKG QAA AGA GM
Sbjct: 400 SLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGM 459

Query: 424 ILCNDELSGFETIADPHVLPASHINYNDGQAVLSYINSTKNPMGYLIPPSAKVNTKPAPT 483
           +LCND+ SG E I+D HVLPAS I+Y DG+ + SY++STK+P GY+  P+A +NTKPAP 
Sbjct: 460 VLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPF 519

Query: 484 MAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVSPTGEAFDNRTVPFITMSGTSMSC 543
           MA+FSSRGPN I+P I+KPD+TAPGVN+IAAF+EA  PT    DNR  PF T SGTSMSC
Sbjct: 520 MASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSC 579

Query: 544 PHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTMKPMLDGGSPDLAPATPFAYGSGH 603
           PH+SG+VGLL+TLHPHWSP+AI+SAIMT++R R+N  KPM+D        A PF+YGSGH
Sbjct: 580 PHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVD---ESFKKANPFSYGSGH 639

Query: 604 IRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAFSDGP-FKCPPNASILNFNYPSIG 663
           ++P  A  PGLVYDL+  DYL+FLCA GY++  ++ F++ P + C   A++L+FNYPSI 
Sbjct: 640 VQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSIT 699

Query: 664 VQNLNGNVTVTRKLKNVSTPGVYRARIMNPDGVKVSVKPKVLKFERVAEEKSFELTMTG- 723
           V NL G++TVTRKLKNV  P  Y AR   P GV+VSV+PK L F +  E K F++T+   
Sbjct: 700 VPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPL 759

Query: 724 NVPEDQVVDGVLIWTDGKHFVRSPIVI 748
            V     V G L WTD  H+VRSPIV+
Sbjct: 760 PVTPSGYVFGELTWTDSHHYVRSPIVV 774

BLAST of Bhi08G000585 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 809.3 bits (2089), Expect = 2.6e-234
Identity = 411/750 (54.80%), Postives = 533/750 (71.07%), Query Frame = 0

Query: 5   SYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFLGSEEKAKDAIFYSYKKHINGFAATL 64
           SYVV  G+HSH  EITE+ ++RV  +H+  LGSF GS E+A DAIFYSY KHINGFAA L
Sbjct: 31  SYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHL 90

Query: 65  DEEEATKLAEHPEVAAILANRAKALHTTHSWEFMHLEKNGVIPSSSPWRIAKSGKDVIIA 124
           D + A ++++HPEV ++  N+A  LHTT SW+F+ LE N  +PSSS WR A+ G+D IIA
Sbjct: 91  DHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIA 150

Query: 125 NLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETPDRVPCNRKLIGAKYFNKGFIAYLKS 184
           NLDTGVWPESKSF + G+ GP+PS+WKG C ++      CNRKLIGA+YFNKG+ A +  
Sbjct: 151 NLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGH 210

Query: 185 ENSTEFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVFGSGAGTAKGGSPKARVAAYKVC 244
            NS+      +S RD +GHGSHTLSTA G +V G S+FG G GTAKGGSP+ARVAAYKVC
Sbjct: 211 LNSS-----FDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVC 270

Query: 245 W-PLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSDPAEYSEDSIAIAAFHAVKKGIPVV 304
           W P++G  C+D+D++ AFD AIHDG DV+S+SLG +P  +  DS+AI +FHA KK I VV
Sbjct: 271 WPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVV 330

Query: 305 CSAGNSGPDALTASNTAPWILTVGASTLDREFQAPVELQNGHRYMGSSLSK-ALQGNKLY 364
           CSAGNSGP   T SN APW +TVGAST+DREF + + L NG  Y G SLS  AL   K Y
Sbjct: 331 CSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFY 390

Query: 365 PLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILACLRGDNARVDKGEQAALAGAVGM 424
           P++    AKAKNA+  DA LCK  +LD  K KGKIL CLRG N RV+KG   AL G +GM
Sbjct: 391 PIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGM 450

Query: 425 ILCNDELSGFETIADPHVLPASHINYNDGQAVLSYINSTKNPMGYLIPPSAKVNTKPAPT 484
           +L N  ++G + +ADPHVLPA+ +   D  AV  YI+ TK P+ ++ P    +  KPAP 
Sbjct: 451 VLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPV 510

Query: 485 MAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVSPTGEAFDNRTVPFITMSGTSMSC 544
           MA+FSS+GP++++P+I+KPD+TAPGV+VIAA++ AVSPT E FD R + F  +SGTSMSC
Sbjct: 511 MASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSC 570

Query: 545 PHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTMKPMLDGGSPDLAPATPFAYGSGH 604
           PH+SG+ GLL+T +P WSP+AI+SAIMT+A I D+   P+ +  +     ATPF++G+GH
Sbjct: 571 PHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATN---MKATPFSFGAGH 630

Query: 605 IRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAFSDGPFKC-PPNASILNFNYPSIG 664
           ++P  A++PGLVYDL   DYL FLC+ GY+   I  FS   F C  P  S++N NYPSI 
Sbjct: 631 VQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSIT 690

Query: 665 VQNL-NGNVTVTRKLKNVSTPGVYRARIMNPDGVKVSVKPKVLKFERVAEEKSFELTMT- 724
           V NL +  VTV+R +KNV  P +Y  ++ NP GV V+VKP  L F +V E+K+F++ +  
Sbjct: 691 VPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVK 750

Query: 725 --GNVPEDQVVDGVLIWTDGKHFVRSPIVI 748
             GNV +  V  G L+W+D KH VRSPIV+
Sbjct: 751 SKGNVAKGYVF-GELVWSDKKHRVRSPIVV 770

BLAST of Bhi08G000585 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 570.1 bits (1468), Expect = 2.7e-162
Identity = 328/709 (46.26%), Postives = 437/709 (61.64%), Query Frame = 0

Query: 49  IFYSYKKHINGFAATLDEEEATKLAEHPEVAAILANRAKALHTTHSWEFMHLEKNGVIPS 108
           + Y+Y+  I+GF+  L +EEA  L   P V ++L      LHTT +  F+ L+++    +
Sbjct: 65  LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH----T 124

Query: 109 SSPWRIAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETPDRVP-CNRK 168
           +  +  A S  DV++  LDTGVWPESKS+ + G  GP+PS WKGGC   T      CNRK
Sbjct: 125 ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGTNFTASLCNRK 184

Query: 169 LIGAKYFNKGFIAYLKSENSTEFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVFGSGAG 228
           LIGA++F +G   Y  +    + S    S RD +GHG+HT STA GS V GAS+ G  +G
Sbjct: 185 LIGARFFARG---YESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASG 244

Query: 229 TAKGGSPKARVAAYKVCWPLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSDPAEYSEDS 288
           TA+G +P+ARVA YKVCW    GGCF SDI+ A D AI D V+VLS+SLG   ++Y  D 
Sbjct: 245 TARGMAPRARVAVYKVCWL---GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDG 304

Query: 289 IAIAAFHAVKKGIPVVCSAGNSGPDALTASNTAPWILTVGASTLDREFQAPVELQNGHRY 348
           +AI AF A+++GI V CSAGN+GP + + SN APWI TVGA TLDR+F A   L NG  +
Sbjct: 305 VAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNF 364

Query: 349 MGSSLSKA-LQGNKLYPLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILACLRGDNA 408
            G SL K     +KL P I      A NA   +  LC   TL   KVKGKI+ C RG NA
Sbjct: 365 TGVSLFKGEALPDKLLPFIYA--GNASNAT--NGNLCMTGTLIPEKVKGKIVMCDRGINA 424

Query: 409 RVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVLSYINSTKNPMG 468
           RV KG+    AG VGMIL N   +G E +AD H+LPA+ +    G  +  Y+ +  NP  
Sbjct: 425 RVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTA 484

Query: 469 YLIPPSAKVNTKPAPTMAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVSPTGEAFD 528
            +      V  KP+P +AAFSSRGPN I+P I+KPD+ APGVN++AA++ A  PTG A D
Sbjct: 485 SISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASD 544

Query: 529 NRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTMKPMLDGG 588
           +R V F  +SGTSMSCPHVSGL  LL+++HP WSP+AI+SA+MT+A       KP+LD  
Sbjct: 545 SRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIA 604

Query: 589 SPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAFSDGPFKC 648
           +    P+TPF +G+GH+ PT A +PGL+YDL+  DYL FLCA  Y    IR+ S   + C
Sbjct: 605 TG--KPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTC 664

Query: 649 PPNA--SILNFNYPSIGVQNLN--GNVTVTRKLKNVSTPGVYRARIMN-PDGVKVSVKPK 708
            P+   S+ + NYPS  V N++  G    TR + +V   G Y  ++ +   GVK+SV+P 
Sbjct: 665 DPSKSYSVADLNYPSFAV-NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPA 724

Query: 709 VLKFERVAEEKSFELTMT--GNVPEDQVVDGVLIWTDGKHFVRSPIVIS 749
           VL F+   E+KS+ +T T   + P      G + W+DGKH V SP+ IS
Sbjct: 725 VLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Bhi08G000585 vs. TAIR 10
Match: AT5G45650.1 (subtilase family protein )

HSP 1 Score: 539.7 bits (1389), Expect = 3.9e-153
Identity = 321/782 (41.05%), Postives = 440/782 (56.27%), Query Frame = 0

Query: 4   QSYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFLGSEEKAKDAIFYSYKKHINGFAAT 63
           Q Y+V  G H       ++    +   HH  L S   SEE A+ ++ YSYK  INGFAA 
Sbjct: 25  QVYIVYFGEHK-----GDKAFHEIEEHHHSYLQSVKESEEDARASLLYSYKHSINGFAAE 84

Query: 64  LDEEEATKLAEHPEVAAILAN--RAKALHTTHSWEFMHLEK---NGVIP-----SSSPWR 123
           L  ++A+KL +  EV ++  +  R    HTT SWEF+ LE+   +  +P     +   +R
Sbjct: 85  LTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSDVPRRKNDADDRFR 144

Query: 124 I-------AKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETP-DRVPCN 183
           +       AK G  +I+  LD+GVWPESKSF + G +GPVP  WKG C      +   CN
Sbjct: 145 VGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKG-MGPVPKSWKGICQTGVAFNSSHCN 204

Query: 184 RKLIGAKYFNKGFIAYLKSENSTEFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVFGSG 243
           RK+IGA+Y+ KG+  Y  + N+T     + S RD +GHGSHT STA G  V GAS  G  
Sbjct: 205 RKIIGARYYVKGYERYYGAFNATANKDFL-SPRDPDGHGSHTASTAVGRRVLGASALGGF 264

Query: 244 A-GTAKGGSPKARVAAYKVCW------PLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLG- 303
           A G+A GG+P AR+A YK CW       +EG  C + D++ A D AI DGV V+S+S+G 
Sbjct: 265 AKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIADGVHVISISIGT 324

Query: 304 SDPAEYSEDSIAIAAFHAVKKGIPVVCSAGNSGPDALTASNTAPWILTVGASTLDREFQA 363
           ++P  +++D IA+ A HAVK+ I V  SAGNSGP   T SN APWI+TVGASTLDR F  
Sbjct: 325 TEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIITVGASTLDRAFVG 384

Query: 364 PVELQNGHRYMGSSLSKALQGNKLYPLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKI 423
            + L NG+     S++ A + +K  PL+  +       A+++   C P +L    V GK+
Sbjct: 385 GLVLGNGYTIKTDSIT-AFKMDKFAPLVYASNVVVPGIALNETSQCLPNSLKPELVSGKV 444

Query: 424 LACLRGDNARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVLSY 483
           + CLRG  +R+ KG +   AG  GMIL N   +G E  +D H +P + +       +L Y
Sbjct: 445 VLCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVPTAGVTPTVVDKILEY 504

Query: 484 INSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEA 543
           I + KNP  ++ P       + AP+M  FSSRGPNV+ P I+KPD+TAPG+ ++AA+S A
Sbjct: 505 IKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPDITAPGLYILAAWSGA 564

Query: 544 VSPTGEAFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDN 603
            SP+  + D R   +   SGTSMSCPHV+G + LL+ +HP WS +AI+SA+MT+A + ++
Sbjct: 565 DSPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWSSAAIRSALMTTAWMTND 624

Query: 604 TMKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIR 663
             KP+ D       PA PFA GSGH RPT A DPGLVYD S   YL + C+    +    
Sbjct: 625 KKKPIQDTTG---LPANPFALGSGHFRPTKAADPGLVYDASYRAYLLYGCSVNITN---- 684

Query: 664 AFSDGPFKCPPNASI-LNFNYPSIGVQNLNGNVTVTRKLKNVST---PGVYRARIMNPDG 723
              D  FKCP       N NYPSI V NL   VTV R + NV T      Y   +  P G
Sbjct: 685 --IDPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVGTGNSTSTYLFSVKPPSG 744

Query: 724 VKVSVKPKVLKFERVAEEKSFELT-------MTGNVPEDQVVDGVLIWTDGKHFVRSPIV 749
           + V   P +L F R+ +++ F++        +     + Q   G   WTD  H VRSPI 
Sbjct: 745 ISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFGWFSWTDKVHVVRSPIA 789

BLAST of Bhi08G000585 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 538.9 bits (1387), Expect = 6.6e-153
Identity = 318/733 (43.38%), Postives = 438/733 (59.75%), Query Frame = 0

Query: 41  SEEKAKDAIFYSYKKHINGFAATLDEEEATKLAEHPEVAAILANRAKALHTTHSWEFMHL 100
           +E   +  I + Y    +GF+A +  +EA  L  HP V A+  +R + LHTT S +F+ L
Sbjct: 50  TEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGL 109

Query: 101 EKNGVIPSSSPWRIAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETPD 160
           +          W  +  G DVII   DTG+WPE +SF +  + GP+P +W+G C  E+  
Sbjct: 110 QN-----QKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNL-GPIPKRWRGVC--ESGA 169

Query: 161 RVP---CNRKLIGAKYFNKG-FIAYLKSENST-EFSSVINSTRDYEGHGSHTLSTAGGSY 220
           R     CNRK+IGA++F KG   A +   N T EF     S RD +GHG+HT STA G +
Sbjct: 170 RFSPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFL----SPRDADGHGTHTSSTAAGRH 229

Query: 221 VSGASVFGSGAGTAKGGSPKARVAAYKVCWPLEGGGCFDSDIVEAFDHAIHDGVDVLSLS 280
              AS+ G  +G AKG +PKAR+AAYKVCW  +  GC DSDI+ AFD A+ DGVDV+S+S
Sbjct: 230 AFKASMSGYASGVAKGVAPKARIAAYKVCW--KDSGCLDSDILAAFDAAVRDGVDVISIS 289

Query: 281 LGSD---PAEYSEDSIAIAAFHAVKKGIPVVCSAGNSGPDALTASNTAPWILTVGASTLD 340
           +G      + Y  D IAI ++ A  KGI V  SAGN GP+ ++ +N APW+ TVGAST+D
Sbjct: 290 IGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTID 349

Query: 341 REFQAPVELQNGHRYMGSSLSKALQGN-KLYPLITGAQAKAKNAAMDDAMLCKPETLDHS 400
           R F A   L +GHR  G SL   +  N +++P++   +     + M  A LC   TLD  
Sbjct: 350 RNFPADAILGDGHRLRGVSLYAGVPLNGRMFPVVYPGK-----SGMSSASLCMENTLDPK 409

Query: 401 KVKGKILACLRGDNARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDG 460
           +V+GKI+ C RG + RV KG     AG VGMIL N   +G   + D H++PA  +  N+G
Sbjct: 410 QVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEG 469

Query: 461 QAVLSYINSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNVISPEIIKPDVTAPGVNVI 520
             + +Y +S  NP+  +      V  KPAP +A+FS RGPN +SPEI+KPD+ APGVN++
Sbjct: 470 DRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNIL 529

Query: 521 AAFSEAVSPTGEAFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTS 580
           AA+++AV PTG   D R   F  +SGTSM+CPHVSG   LL++ HP WSP+ I+SA+MT+
Sbjct: 530 AAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTT 589

Query: 581 ARIRDNTMKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGY 640
             + DN+ + ++D  +     ATP+ YGSGH+    A++PGLVYD++ +DY+ FLC+ GY
Sbjct: 590 TNLVDNSNRSLIDESTG--KSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGY 649

Query: 641 DDKTIRAFSDGPFKCPP--NASILNFNYPSIGV---QNLNGNV--TVTRKLKNV-STPGV 700
             KTI+  +  P +CP     S  N NYPSI      N  G V  TV R   NV     V
Sbjct: 650 GPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAV 709

Query: 701 YRARIMNPDGVKVSVKPKVLKFERVAEEKSFELTMTGN-----VPEDQVVDGVLIWTD-G 751
           YRARI +P GV V+VKP  L F    + +S+ +T+T N     + E   V G + W D G
Sbjct: 710 YRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGG 761

BLAST of Bhi08G000585 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 891.0 bits (2301), Expect = 9.6e-258
Identity = 444/747 (59.44%), Postives = 553/747 (74.03%), Query Frame = 0

Query: 4   QSYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFLGSEEKAKDAIFYSYKKHINGFAAT 63
           +SY+V LGSH+H  +I+   L+ V HSH   L SF+GS E AK+AIFYSYK+HINGFAA 
Sbjct: 40  KSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAI 99

Query: 64  LDEEEATKLAEHPEVAAILANRAKALHTTHSWEFMHLEKNGVIPSSSPWRIAKSGKDVII 123
           LDE EA ++A+HP+V ++  N+ + LHTTHSW FM L KNGV+  SS W  A  G+D II
Sbjct: 100 LDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTII 159

Query: 124 ANLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETPDRVPCNRKLIGAKYFNKGFIAYLK 183
           ANLDTGVWPESKSF + G  G VP++WKG C  +    VPCNRKLIGA+YFNKG++AY  
Sbjct: 160 ANLDTGVWPESKSFSDEG-YGAVPARWKGRCHKD----VPCNRKLIGARYFNKGYLAYTG 219

Query: 184 SENSTEFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVFGSGAGTAKGGSPKARVAAYKV 243
             ++  +     + RD++GHGSHTLSTA G++V GA+VFG G GTA GGSPKARVAAYKV
Sbjct: 220 LPSNASY----ETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKV 279

Query: 244 CW-PLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSDPAEYSEDSIAIAAFHAVKKGIPV 303
           CW P++G  CFD+DI+ A + AI DGVDVLS S+G D  +Y  D IAI +FHAVK G+ V
Sbjct: 280 CWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTV 339

Query: 304 VCSAGNSGPDALTASNTAPWILTVGASTLDREFQAPVELQNGHRYMGSSLSKALQGNKLY 363
           VCSAGNSGP + T SN APW++TVGAS++DREFQA VEL+NG  + G+SLSK L   K+Y
Sbjct: 340 VCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLSKPLPEEKMY 399

Query: 364 PLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILACLRGDNARVDKGEQAALAGAVGM 423
            LI+ A A   N  + DA+LCK  +LD  KVKGKIL CLRGDNARVDKG QAA AGA GM
Sbjct: 400 SLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGM 459

Query: 424 ILCNDELSGFETIADPHVLPASHINYNDGQAVLSYINSTKNPMGYLIPPSAKVNTKPAPT 483
           +LCND+ SG E I+D HVLPAS I+Y DG+ + SY++STK+P GY+  P+A +NTKPAP 
Sbjct: 460 VLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPF 519

Query: 484 MAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVSPTGEAFDNRTVPFITMSGTSMSC 543
           MA+FSSRGPN I+P I+KPD+TAPGVN+IAAF+EA  PT    DNR  PF T SGTSMSC
Sbjct: 520 MASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSC 579

Query: 544 PHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTMKPMLDGGSPDLAPATPFAYGSGH 603
           PH+SG+VGLL+TLHPHWSP+AI+SAIMT++R R+N  KPM+D        A PF+YGSGH
Sbjct: 580 PHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVD---ESFKKANPFSYGSGH 639

Query: 604 IRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAFSDGP-FKCPPNASILNFNYPSIG 663
           ++P  A  PGLVYDL+  DYL+FLCA GY++  ++ F++ P + C   A++L+FNYPSI 
Sbjct: 640 VQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSIT 699

Query: 664 VQNLNGNVTVTRKLKNVSTPGVYRARIMNPDGVKVSVKPKVLKFERVAEEKSFELTMTG- 723
           V NL G++TVTRKLKNV  P  Y AR   P GV+VSV+PK L F +  E K F++T+   
Sbjct: 700 VPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPL 759

Query: 724 NVPEDQVVDGVLIWTDGKHFVRSPIVI 748
            V     V G L WTD  H+VRSPIV+
Sbjct: 760 PVTPSGYVFGELTWTDSHHYVRSPIVV 774

BLAST of Bhi08G000585 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 3.7e-233
Identity = 411/750 (54.80%), Postives = 533/750 (71.07%), Query Frame = 0

Query: 5   SYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFLGSEEKAKDAIFYSYKKHINGFAATL 64
           SYVV  G+HSH  EITE+ ++RV  +H+  LGSF GS E+A DAIFYSY KHINGFAA L
Sbjct: 31  SYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHL 90

Query: 65  DEEEATKLAEHPEVAAILANRAKALHTTHSWEFMHLEKNGVIPSSSPWRIAKSGKDVIIA 124
           D + A ++++HPEV ++  N+A  LHTT SW+F+ LE N  +PSSS WR A+ G+D IIA
Sbjct: 91  DHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIA 150

Query: 125 NLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETPDRVPCNRKLIGAKYFNKGFIAYLKS 184
           NLDTGVWPESKSF + G+ GP+PS+WKG C ++      CNRKLIGA+YFNKG+ A +  
Sbjct: 151 NLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGH 210

Query: 185 ENSTEFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVFGSGAGTAKGGSPKARVAAYKVC 244
            NS+      +S RD +GHGSHTLSTA G +V G S+FG G GTAKGGSP+ARVAAYKVC
Sbjct: 211 LNSS-----FDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVC 270

Query: 245 W-PLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSDPAEYSEDSIAIAAFHAVKKGIPVV 304
           W P++G  C+D+D++ AFD AIHDG DV+S+SLG +P  +  DS+AI +FHA KK I VV
Sbjct: 271 WPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVV 330

Query: 305 CSAGNSGPDALTASNTAPWILTVGASTLDREFQAPVELQNGHRYMGSSLSK-ALQGNKLY 364
           CSAGNSGP   T SN APW +TVGAST+DREF + + L NG  Y G SLS  AL   K Y
Sbjct: 331 CSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFY 390

Query: 365 PLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILACLRGDNARVDKGEQAALAGAVGM 424
           P++    AKAKNA+  DA LCK  +LD  K KGKIL CLRG N RV+KG   AL G +GM
Sbjct: 391 PIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGM 450

Query: 425 ILCNDELSGFETIADPHVLPASHINYNDGQAVLSYINSTKNPMGYLIPPSAKVNTKPAPT 484
           +L N  ++G + +ADPHVLPA+ +   D  AV  YI+ TK P+ ++ P    +  KPAP 
Sbjct: 451 VLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPV 510

Query: 485 MAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVSPTGEAFDNRTVPFITMSGTSMSC 544
           MA+FSS+GP++++P+I+KPD+TAPGV+VIAA++ AVSPT E FD R + F  +SGTSMSC
Sbjct: 511 MASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSC 570

Query: 545 PHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTMKPMLDGGSPDLAPATPFAYGSGH 604
           PH+SG+ GLL+T +P WSP+AI+SAIMT+A I D+   P+ +  +     ATPF++G+GH
Sbjct: 571 PHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATN---MKATPFSFGAGH 630

Query: 605 IRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAFSDGPFKC-PPNASILNFNYPSIG 664
           ++P  A++PGLVYDL   DYL FLC+ GY+   I  FS   F C  P  S++N NYPSI 
Sbjct: 631 VQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSIT 690

Query: 665 VQNL-NGNVTVTRKLKNVSTPGVYRARIMNPDGVKVSVKPKVLKFERVAEEKSFELTMT- 724
           V NL +  VTV+R +KNV  P +Y  ++ NP GV V+VKP  L F +V E+K+F++ +  
Sbjct: 691 VPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVK 750

Query: 725 --GNVPEDQVVDGVLIWTDGKHFVRSPIVI 748
             GNV +  V  G L+W+D KH VRSPIV+
Sbjct: 751 SKGNVAKGYVF-GELVWSDKKHRVRSPIVV 770

BLAST of Bhi08G000585 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 641.0 bits (1652), Expect = 1.7e-182
Identity = 364/767 (47.46%), Postives = 476/767 (62.06%), Query Frame = 0

Query: 6   YVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFLGSEEKAKDAIFYSYKKHINGFAATLD 65
           Y+V +G+HSHG   T  DLE    SH+ LLGS  GS EKAK+AI YSY +HINGFAA L+
Sbjct: 32  YIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREKAKEAIIYSYNRHINGFAALLE 91

Query: 66  EEEATKLAEHPEVAAILANRAKALHTTHSWEFMHLEKNGVIPSSSPWRIAKSGKDVIIAN 125
           EEEA  +A++P V ++  ++   LHTT SWEF+ L + G    +S W+  + G++ II N
Sbjct: 92  EEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRG---QNSAWQKGRFGENTIIGN 151

Query: 126 LDTGVWPESKSFGEHGIVGPVPSKWKGGC--MDETPDRV--PCNRKLIGAKYFNKGFIAY 185
           +DTGVWPES+SF + G  G VPSKW+GG   +++ P  +   CNRKLIGA+Y+NK F A+
Sbjct: 152 IDTGVWPESQSFSDKG-YGTVPSKWRGGLCQINKLPGSMKNTCNRKLIGARYYNKAFEAH 211

Query: 186 LKSENSTEFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVFGSGAGTAKGGSPKARVAAY 245
                + +   ++++ RD+ GHG+HTLSTAGG++V GA VF  G GTAKGGSP+ARVAAY
Sbjct: 212 -----NGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAVGNGTAKGGSPRARVAAY 271

Query: 246 KVCWPL-EGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSDPAEYSE----DSIAIAAFHAV 305
           KVCW L +   C+ +D++ A D AI DGVDV+++S G      +E    D I+I AFHA+
Sbjct: 272 KVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGIFTDEISIGAFHAI 331

Query: 306 KKGIPVVCSAGNSGPDALTASNTAPWILTVGASTLDREFQAPVELQNGHRYMGSSLSKAL 365
            K I +V SAGN GP   T +N APW+ T+ ASTLDR+F + + + N     G+SL   L
Sbjct: 332 SKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTI-NNQLIEGASLFVNL 391

Query: 366 QGNKLYPLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILACLR-GDNARVDKGEQAA 425
             N+ + LI    AK  NA   DA LC+  TLD +KV GKI+ C R G    V +G +A 
Sbjct: 392 PPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVLCTREGKIKSVAEGLEAL 451

Query: 426 LAGAVGMILCNDELSGFETIADPHVL-----PASHINYNDGQAVLSYINSTKNPM--GYL 485
            AGA GMIL N   +G    A+PHV      P             + I    +P+  G  
Sbjct: 452 TAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVKTTAIGDEDDPLKTGDT 511

Query: 486 IPPS---AKVNTKPAPTMAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVSPTGEAF 545
           I  S        KPAP MA+FSSRGPN I P I+KPDVTAPGVN++AA+SE  S +    
Sbjct: 512 IKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNILAAYSEFASASSLLV 571

Query: 546 DNRT-VPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTMKPMLD 605
           DNR    F  + GTSMSCPH SG+ GLL+T HP WSP+AIKSAIMT+A   DNT +P+ D
Sbjct: 572 DNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLDNTNRPIQD 631

Query: 606 GGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAFS-DGP 665
                LA A  FAYGSGH+RP  AI+PGLVYDLS  DYL FLCASGYD + I A + +  
Sbjct: 632 AFDKTLADA--FAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQLISALNFNRT 691

Query: 666 FKCPPNASILNFNYPSIGVQNLN-GNVTVTRKLKNVSTPGVYRARIMNPDGVKVSVKPKV 725
           F C  + S+ + NYPSI + NL    VT+ R + NV  P  Y     +P+G  ++V P  
Sbjct: 692 FICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPSTYTVSTRSPNGYSIAVVPPS 751

Query: 726 LKFERVAEEKSFELTMTGN--VPEDQVVDGVLIWTDGKHFVRSPIVI 748
           L F ++ E K+F++ +  +      +   G L WTDGKH VRSPI +
Sbjct: 752 LTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVRSPITV 786

BLAST of Bhi08G000585 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 3.7e-161
Identity = 328/709 (46.26%), Postives = 437/709 (61.64%), Query Frame = 0

Query: 49  IFYSYKKHINGFAATLDEEEATKLAEHPEVAAILANRAKALHTTHSWEFMHLEKNGVIPS 108
           + Y+Y+  I+GF+  L +EEA  L   P V ++L      LHTT +  F+ L+++    +
Sbjct: 65  LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH----T 124

Query: 109 SSPWRIAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETPDRVP-CNRK 168
           +  +  A S  DV++  LDTGVWPESKS+ + G  GP+PS WKGGC   T      CNRK
Sbjct: 125 ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGTNFTASLCNRK 184

Query: 169 LIGAKYFNKGFIAYLKSENSTEFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVFGSGAG 228
           LIGA++F +G   Y  +    + S    S RD +GHG+HT STA GS V GAS+ G  +G
Sbjct: 185 LIGARFFARG---YESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASG 244

Query: 229 TAKGGSPKARVAAYKVCWPLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSDPAEYSEDS 288
           TA+G +P+ARVA YKVCW    GGCF SDI+ A D AI D V+VLS+SLG   ++Y  D 
Sbjct: 245 TARGMAPRARVAVYKVCWL---GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDG 304

Query: 289 IAIAAFHAVKKGIPVVCSAGNSGPDALTASNTAPWILTVGASTLDREFQAPVELQNGHRY 348
           +AI AF A+++GI V CSAGN+GP + + SN APWI TVGA TLDR+F A   L NG  +
Sbjct: 305 VAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNF 364

Query: 349 MGSSLSKA-LQGNKLYPLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILACLRGDNA 408
            G SL K     +KL P I      A NA   +  LC   TL   KVKGKI+ C RG NA
Sbjct: 365 TGVSLFKGEALPDKLLPFIYA--GNASNAT--NGNLCMTGTLIPEKVKGKIVMCDRGINA 424

Query: 409 RVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVLSYINSTKNPMG 468
           RV KG+    AG VGMIL N   +G E +AD H+LPA+ +    G  +  Y+ +  NP  
Sbjct: 425 RVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTA 484

Query: 469 YLIPPSAKVNTKPAPTMAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVSPTGEAFD 528
            +      V  KP+P +AAFSSRGPN I+P I+KPD+ APGVN++AA++ A  PTG A D
Sbjct: 485 SISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASD 544

Query: 529 NRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTMKPMLDGG 588
           +R V F  +SGTSMSCPHVSGL  LL+++HP WSP+AI+SA+MT+A       KP+LD  
Sbjct: 545 SRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIA 604

Query: 589 SPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAFSDGPFKC 648
           +    P+TPF +G+GH+ PT A +PGL+YDL+  DYL FLCA  Y    IR+ S   + C
Sbjct: 605 TG--KPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTC 664

Query: 649 PPNA--SILNFNYPSIGVQNLN--GNVTVTRKLKNVSTPGVYRARIMN-PDGVKVSVKPK 708
            P+   S+ + NYPS  V N++  G    TR + +V   G Y  ++ +   GVK+SV+P 
Sbjct: 665 DPSKSYSVADLNYPSFAV-NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPA 724

Query: 709 VLKFERVAEEKSFELTMT--GNVPEDQVVDGVLIWTDGKHFVRSPIVIS 749
           VL F+   E+KS+ +T T   + P      G + W+DGKH V SP+ IS
Sbjct: 725 VLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Bhi08G000585 vs. ExPASy Swiss-Prot
Match: A9JQS7 (Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 1.9e-152
Identity = 319/750 (42.53%), Postives = 447/750 (59.60%), Query Frame = 0

Query: 6   YVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFLGSEEKAKDAIFYSYKKHINGFAATLD 65
           Y+V +G  SH       + E VV ++H++L S  GS   AK A  + Y +   GF+A + 
Sbjct: 28  YIVYMGDRSH------PNSESVVRANHEILASVTGSLNDAKAAAIHHYSRSFQGFSAMIT 87

Query: 66  EEEATKLAEHPEVAAILANRAKALHTTHSWEFMHLEKNGVIPSSSPWRIAKSGKDVIIAN 125
            E+A KLA+H  V ++  ++   LHTTHSW+F+ L+    +  ++P  +  S  +VI+  
Sbjct: 88  PEQAKKLADHNSVVSVFESKMNKLHTTHSWDFLGLD---TVYKNNPSAL-DSASNVIVGV 147

Query: 126 LDTGVWPESKSFGEHGIVGPVPSKWKGGCM-DETPDRVPCNRKLIGAKYFNKGFIAYLKS 185
           +D+GVWPES+SF ++G+ GPVP K+KG C+  +      CN+K+IGA++++KG  A +  
Sbjct: 148 IDSGVWPESESFNDYGL-GPVPEKFKGECVTGDNFTLANCNKKIIGARFYSKGLEAEIGP 207

Query: 186 ENSTEFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVFGSGAGTAKGGSPKARVAAYKVC 245
             +   S    S RD +GHG+HT ST  GS VS  S+FG   GTA+GG+P AR++ YK C
Sbjct: 208 LENIVDSIFFRSPRDSDGHGTHTASTIAGSIVSNVSLFGMAKGTARGGAPSARLSIYKAC 267

Query: 246 WPLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSDPAE--YSEDSIAIAAFHAVKKGIPV 305
           W    G C D+D+  A D AIHDGVD+LSLSLG DP +  Y E++I++ AFHA +KGI V
Sbjct: 268 W---FGFCSDADVFAAMDDAIHDGVDILSLSLGPDPPQPLYFENAISVGAFHAFQKGILV 327

Query: 306 VCSAGNSGPDALTASNTAPWILTVGASTLDREFQAPVELQNGHRYMGSSLSK-ALQGNKL 365
             SAGNS     TA N APWI TV AST+DREF++ + L N     G SL+   ++G+  
Sbjct: 328 SASAGNS-VFPRTACNVAPWIFTVAASTVDREFRSDIYLGNSKVLKGLSLNPIKMEGS-- 387

Query: 366 YPLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILACL--RGDNARVDKGEQAALAGA 425
           Y LI G+ A A   A  +A  CK  TLD + +KGKI+ C   +  + R +K       G 
Sbjct: 388 YGLIYGSAAAAAGDAALNASFCKEHTLDPTLIKGKIVICTVEKFTDNRREKAIIIKQGGG 447

Query: 426 VGMILCNDELSGFETIADPHVLPASHINYNDGQAVLSYINSTKNPMGYLIPPSAKVNTKP 485
           VGMIL +        +    V+P++ I  +  + + +Y+ + KNP   + P    V TKP
Sbjct: 448 VGMILIDHNA---RDVGFQFVIPSTMIGQDAVEELQAYMKTEKNPTATIFPTLTLVGTKP 507

Query: 486 APTMAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVSPTGEAFDNRTVPFITMSGTS 545
           AP  AAFSS GPN+I+P+IIKPD+T PGVN++AA+S     T    + ++V +  +SGTS
Sbjct: 508 APESAAFSSVGPNIITPDIIKPDITGPGVNILAAWSPVA--TEATVEQKSVNYNIISGTS 567

Query: 546 MSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTMKPMLDGGSPDLAPATPFAYG 605
           MSCPH+S +  ++++ HP WSP+AI SAIMTSA + DNT    L G  P+   ATPF YG
Sbjct: 568 MSCPHISAISAIIKSHHPSWSPAAIMSAIMTSATVMDNTHS--LIGRDPNGTQATPFDYG 627

Query: 606 SGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAFSDGPFKC--PPNASILNFNY 665
           SGH+ P  +++PGLVYD S  D L FLC++G     ++  +    +C   P AS  NFNY
Sbjct: 628 SGHVNPVASLNPGLVYDFSSQDVLNFLCSNGASPAQLKNLTGELTQCQKSPTAS-YNFNY 687

Query: 666 PSIGVQNLNGNVTVTRKLKNV-STPGVYRARIMNPDGVKVSVKPKVLKFERVAEEKSFEL 725
           PSIGV NLNG+++V R +      P  Y A +  P GV V V P  LKF +  E+ +F +
Sbjct: 688 PSIGVSNLNGSLSVYRTVTYYGQEPTEYFASVERPSGVIVRVTPAKLKFWKAGEKITFRI 747

Query: 726 TMTG-NVPEDQVVDGVLIWTDGKHFVRSPI 746
             T         V G L W +GK  VRSPI
Sbjct: 748 DFTPFKNSNGNFVFGALTWNNGKQRVRSPI 752

BLAST of Bhi08G000585 vs. ExPASy TrEMBL
Match: A0A0A0LVY8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1)

HSP 1 Score: 1344.3 bits (3478), Expect = 0.0e+00
Identity = 657/752 (87.37%), Postives = 705/752 (93.75%), Query Frame = 0

Query: 1   MGFQSYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFLGSEEKAKDAIFYSYKKHINGF 60
           +GFQSY+VLLGSHSHG+E+T+EDL+RV  SHHKLLGS  GS+EKA++AIFYSYKK+INGF
Sbjct: 8   LGFQSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNINGF 67

Query: 61  AATLDEEEATKLAEHPEVAAILANRAKALHTTHSWEFMHLEKNGVIPSSSPWRIAKSGKD 120
           AA +DEEEA +LA+HPEVAA+L NRAK LHTTHSWEFMHLEKNGVIP SS WR AKSGKD
Sbjct: 68  AAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKD 127

Query: 121 VIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETPDRVPCNRKLIGAKYFNKGFIA 180
           VIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC D+T DRVPCNRKLIGAKYFNKGF+A
Sbjct: 128 VIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGFLA 187

Query: 181 YLKSENSTEFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVFGSGAGTAKGGSPKARVAA 240
           YLKSEN T  + VINSTRDY+GHGSHTLSTAGGSYVSGASVFG G GTAKGGSPKARVAA
Sbjct: 188 YLKSENLT--ALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVAA 247

Query: 241 YKVCWPLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSDPAEYSEDSIAIAAFHAVKKGI 300
           YKVCWPLE GGCFD+DI +AFDHAIHD VDVLSLSLG +PA+Y +D IAI+AFHAVKKGI
Sbjct: 248 YKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKGI 307

Query: 301 PVVCSAGNSGPDALTASNTAPWILTVGASTLDREFQAPVELQNGHRYMGSSLSKALQGNK 360
           PVVCSAGNSGP A T SNTAPWILTVGAST+DREFQAPVELQNGHRYMGSSLSK L+G+K
Sbjct: 308 PVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGDK 367

Query: 361 LYPLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILACLRGDNARVDKGEQAALAGAV 420
           LYPLITGA+AKAKNA  ++A LCKP+TLDHSKVKGKIL CLRGD ARVDKGEQAALAGAV
Sbjct: 368 LYPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAV 427

Query: 421 GMILCNDELSGFETIADPHVLPASHINYNDGQAVLSYINSTKNPMGYLIPPSAKVNTKPA 480
           GMILCNDELSGFETIADPHVLPASHINYNDGQAV SYI +TKNPMGYLIPP+AKVNTKPA
Sbjct: 428 GMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKVNTKPA 487

Query: 481 PTMAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVSPTGEAFDNRTVPFITMSGTSM 540
           PTMAAFSSRGPN+ISPEIIKPDVTAPGVN+IAAFSEAVSPTGE FDNRTVPFITMSGTSM
Sbjct: 488 PTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSM 547

Query: 541 SCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTMKPMLDGGSPDLAPATPFAYGS 600
           SCPHVSGLVGLLRTLHP WSPSAIKSAIMTSARIRDNT KPMLDGGSPDLAP+TPFAYGS
Sbjct: 548 SCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGS 607

Query: 601 GHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAFSDGPFKCPPNASILNFNYPSI 660
           GHIRPTGAIDPGLVYDLSPNDYLEFLCASGY++KTI+AFSDGPFKCP +ASILN NYPSI
Sbjct: 608 GHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSI 667

Query: 661 GVQNLNGNVTVTRKLKNVSTPGVYRARIMNPDGVKVSVKPKVLKFERVAEEKSFELTMTG 720
           GVQNL G+VTVTRKLKNVSTPGVY+ R+ +P+GVKV VKPKVLKFERV EEKSFELT+TG
Sbjct: 668 GVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITG 727

Query: 721 NVPEDQVVDGVLIWTDGKHFVRSPIVISSVLF 753
           +VPEDQVVDGVLIWTDGKHFVRSPIV+SS LF
Sbjct: 728 DVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757

BLAST of Bhi08G000585 vs. ExPASy TrEMBL
Match: A0A1S3CLB7 (subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=1)

HSP 1 Score: 1324.7 bits (3427), Expect = 0.0e+00
Identity = 650/749 (86.78%), Postives = 699/749 (93.32%), Query Frame = 0

Query: 4   QSYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFLGSEEKAKDAIFYSYKKHINGFAAT 63
           +SY+VLLGSHSHG+E+ +EDLERV  SHHKLLGS +GS+EKA+DAIFYSYK++INGFAA 
Sbjct: 30  KSYIVLLGSHSHGLEVRDEDLERVADSHHKLLGSIIGSDEKARDAIFYSYKRNINGFAAI 89

Query: 64  LDEEEATKLAEHPEVAAILANRAKALHTTHSWEFMHLEKNGVIPSSSPWRIAKSGKDVII 123
           +DEEEAT+LA+HPEVAA+L N+AK LHTTHSWEFMHLEKNGVIP SS WR AKSGKDVII
Sbjct: 90  MDEEEATQLAKHPEVAAVLLNKAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVII 149

Query: 124 ANLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETPDRVPCNRKLIGAKYFNKGFIAYLK 183
            NLDTGVW ESKSFGEHGIVG VPSKWKGGC D+TPD V CNRKLIGAKYFNKGF+AYL 
Sbjct: 150 GNLDTGVWGESKSFGEHGIVGAVPSKWKGGCTDKTPDGVSCNRKLIGAKYFNKGFLAYLN 209

Query: 184 SENSTEFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVFGSGAGTAKGGSPKARVAAYKV 243
           S+N T  +SVINSTRDY+GHGSHTLSTAGGSYVSGASVFG G GTAKGGSPKARVA+YKV
Sbjct: 210 SQNLT--ASVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVASYKV 269

Query: 244 CWPLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSDPAEYSEDSIAIAAFHAVKKGIPVV 303
           CWPLE GGCF++DI EAFDHAIHD VDVLSLSLG +PA+Y +D IAIAAFHAVKKGIPVV
Sbjct: 270 CWPLEDGGCFEADIAEAFDHAIHDRVDVLSLSLGGEPADYYDDGIAIAAFHAVKKGIPVV 329

Query: 304 CSAGNSGPDALTASNTAPWILTVGASTLDREFQAPVELQNGHRYMGSSLSKALQGNKLYP 363
           CSAGNSGP A T SNTAPWILTVGASTLDREFQAPVELQNGH YMGSSLSK L+G+KLYP
Sbjct: 330 CSAGNSGPAAQTVSNTAPWILTVGASTLDREFQAPVELQNGHSYMGSSLSKGLKGDKLYP 389

Query: 364 LITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILACLRGDNARVDKGEQAALAGAVGMI 423
           LITGA+AKAKNA  + AMLCKP+TLDHSKVKGKIL CLRGD ARVDKGEQAALAGAVGMI
Sbjct: 390 LITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMI 449

Query: 424 LCNDELSGFETIADPHVLPASHINYNDGQAVLSYINSTKNPMGYLIPPSAKVNTKPAPTM 483
           LCND+LSGFETIADPHVLPASHINYNDGQAV SYI STKNPMG LIPPSAKVNTKPAP+M
Sbjct: 450 LCNDKLSGFETIADPHVLPASHINYNDGQAVFSYIKSTKNPMGSLIPPSAKVNTKPAPSM 509

Query: 484 AAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVSPTGEAFDNRTVPFITMSGTSMSCP 543
           AAFSSRGPN+ISPEIIKPDVTAPGVN+IAAFSEAVSPTGE FDNRTVPFITMSGTSMSCP
Sbjct: 510 AAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCP 569

Query: 544 HVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTMKPMLDGGSPDLAPATPFAYGSGHI 603
           HVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNT KPMLDGGSPDLAPATPFAYGSGHI
Sbjct: 570 HVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHI 629

Query: 604 RPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAFSDGPFKCPPNASILNFNYPSIGVQ 663
           RPTGAIDPGLVYDLSPNDYLEFLCASGY++KTI+AFSDGPFKCP +ASILNFNYPSIGVQ
Sbjct: 630 RPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQ 689

Query: 664 NLNGNVTVTRKLKNVSTPGVYRARIMNPDGVKVSVKPKVLKFERVAEEKSFELTMTGNVP 723
           NL G+VT+TRKLKNVSTPGVY+AR+M+P+GVKV VKPKVLKFERV EEK FEL +TG+VP
Sbjct: 690 NLTGSVTLTRKLKNVSTPGVYKARVMHPNGVKVLVKPKVLKFERVGEEKRFELIITGDVP 749

Query: 724 EDQVVDGVLIWTDGKHFVRSPIVISSVLF 753
           E+QVVDGVLIWTDGKHFVRSPIV+SS LF
Sbjct: 750 ENQVVDGVLIWTDGKHFVRSPIVVSSGLF 776

BLAST of Bhi08G000585 vs. ExPASy TrEMBL
Match: A0A6J1CN43 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012645 PE=3 SV=1)

HSP 1 Score: 1266.5 bits (3276), Expect = 0.0e+00
Identity = 612/751 (81.49%), Postives = 686/751 (91.34%), Query Frame = 0

Query: 4   QSYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFLGSEEKAKDAIFYSYKKHINGFAAT 63
           +SYVV+LGSHSHG+E++E DL+RVV SHHKLLGSFLGS EKAKDAIFYSYKK+INGFAA 
Sbjct: 29  KSYVVILGSHSHGLEVSEADLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKKNINGFAAI 88

Query: 64  LDEEEATKLAEHPEVAAILANRAKALHTTHSWEFMHLEK-NGVIPSSSPWRIAKSGKDVI 123
           L+EEEA +LA+HPEVAA+LAN+ K LHTTHSW FM LEK NGV+P +SPW  A  G+D I
Sbjct: 89  LEEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFMRLEKMNGVVPPTSPWWRANFGQDTI 148

Query: 124 IANLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETPDRVPCNRKLIGAKYFNKGFIAYL 183
           IANLDTGVWPESKSFGE+GIVG VP+KWKGGC D++PDRVPCNRKLIGAKYFNKGFIAYL
Sbjct: 149 IANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDDSPDRVPCNRKLIGAKYFNKGFIAYL 208

Query: 184 KSENST-EFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVFGSGAGTAKGGSPKARVAAY 243
           K+ NS+ + SS+ NSTRDYEGHGSHTLSTAGGS+V  ASVFGSG GTAKGGSPKARVAAY
Sbjct: 209 KALNSSADLSSIKNSTRDYEGHGSHTLSTAGGSFVPDASVFGSGLGTAKGGSPKARVAAY 268

Query: 244 KVCWPLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSDPAEYSEDSIAIAAFHAVKKGIP 303
           K+CWP + GGCFD+DI E FDHAIHDGV+V+SLS+G  PA Y +DSIAIAAFHAVKKGIP
Sbjct: 269 KICWPFQDGGCFDADITEGFDHAIHDGVEVISLSVGGSPANYFDDSIAIAAFHAVKKGIP 328

Query: 304 VVCSAGNSGPDALTASNTAPWILTVGASTLDREFQAPVELQNGHRYMGSSLSKALQGNKL 363
           VVCSAGNSGPDA TASNTAPWILTVGASTLDR+FQAPVEL+NG R+ GSSLS AL  +KL
Sbjct: 329 VVCSAGNSGPDAFTASNTAPWILTVGASTLDRQFQAPVELKNGQRFQGSSLSTALPEDKL 388

Query: 364 YPLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILACLRGDNARVDKGEQAALAGAVG 423
           YPLITGAQAKA+NA+  DAMLCKPETLDHSK KGKILACLRGDNARVDKGEQAALAGA G
Sbjct: 389 YPLITGAQAKAENASAADAMLCKPETLDHSKAKGKILACLRGDNARVDKGEQAALAGAAG 448

Query: 424 MILCNDELSGFETIADPHVLPASHINYNDGQAVLSYINSTKNPMGYLIPPSAKVNTKPAP 483
           MILCNDELSGFETIADPH+LPASH+NYNDGQAVL YI ST+NPMGYLIPP+AK+NTKPAP
Sbjct: 449 MILCNDELSGFETIADPHLLPASHVNYNDGQAVLKYIQSTQNPMGYLIPPAAKLNTKPAP 508

Query: 484 TMAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVSPTGEAFDNRTVPFITMSGTSMS 543
            MAAFSSRGPN+++PEIIKPDVTAPGVNVIAA++EAVSPTGE FDNRT PFITMSGTSMS
Sbjct: 509 VMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMS 568

Query: 544 CPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTMKPMLDGGSPDLAPATPFAYGSG 603
           CPHV+GL GLLR+LHP+WSPSAIKSAIMTSARIRDNTMKPMLDGG+ +LAPATPF+YGSG
Sbjct: 569 CPHVAGLAGLLRSLHPNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSG 628

Query: 604 HIRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAFSDGPFKCPPNASILNFNYPSIG 663
           HIRP GA+DPGLVYDL+P+DYLEFLCA GYD+K IRAFSDGP+KCPP+AS+LNFNYPSIG
Sbjct: 629 HIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNYPSIG 688

Query: 664 VQNLNGNVTVTRKLKNVSTPGVYRARIMNPDGVKVSVKPKVLKFERVAEEKSFELTMTGN 723
           VQN+ G+VTVTR+LKNV TPGVYRAR+  P+GV+VSV+P+ LKF++V EEKSF+LT+ G 
Sbjct: 689 VQNMTGSVTVTRRLKNVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGV 748

Query: 724 VPEDQVVDGVLIWTDGKHFVRSPIVISSVLF 753
           VP  +VVDG LIWTDG+HFVRSPIVISS LF
Sbjct: 749 VPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 779

BLAST of Bhi08G000585 vs. ExPASy TrEMBL
Match: A0A5D3CZ66 (Subtilisin-like protease SBT5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00570 PE=3 SV=1)

HSP 1 Score: 1260.7 bits (3261), Expect = 0.0e+00
Identity = 614/749 (81.98%), Postives = 675/749 (90.12%), Query Frame = 0

Query: 5    SYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFLGSEEKAKDAIFYSYKKHINGFAATL 64
            SYVVLLGSHSHG+E+TE+D E VV SHHKLLGSFL S+EKAKD+IFYSYKKHINGFAATL
Sbjct: 755  SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATL 814

Query: 65   DEEEATKLAEHPEVAAILANRAKALHTTHSWEFMHLEKNGVIPSSSPWRIAKSGKDVIIA 124
            D+E+AT+LA HPEVAA+L N+ K L+TTHSWEFMHLEKNGV+P SSPWR+AK GKDVIIA
Sbjct: 815  DDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDVIIA 874

Query: 125  NLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETPDRVPCNRKLIGAKYFNKGFIAYLKS 184
            NLDTGVWPESKSFGEHGI GP PSKWKGGC D++PD VPCN+KLIGAKYFNKG++ YLKS
Sbjct: 875  NLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYLEYLKS 934

Query: 185  ENST-EFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVFGSGAGTAKGGSPKARVAAYKV 244
            ENST + SS+INSTRDY+GHGSHTLSTA G+YV GASVFGSG GTAKGGSPKARVAAYKV
Sbjct: 935  ENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVAAYKV 994

Query: 245  CWPLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSDPAEYSEDSIAIAAFHAVKKGIPVV 304
            CWP E GGCFD+DI EAFDHAIHDGVDVLSLSLG DP +YSEDSIAIA+FHAVKKGIPVV
Sbjct: 995  CWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKKGIPVV 1054

Query: 305  CSAGNSGPDALTASNTAPWILTVGASTLDREFQAPVELQNGHRYMGSSLSKALQGNKLYP 364
            C+ GNSGP   TASNTAPWILTVGASTLDREF APV LQNGHR+MGSS SK L G KLYP
Sbjct: 1055 CAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTGRKLYP 1114

Query: 365  LITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILACLRGDNARVDKGEQAALAGAVGMI 424
            LITGAQAKA NA  DDAMLCKPETLDHSKVKGKIL CLRG+ AR+DKG+QAALAGAVGMI
Sbjct: 1115 LITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGMI 1174

Query: 425  LCNDELSGFETIADPHVLPASHINYNDGQAVLSYINSTKNPMGYLIPPSAKVNTKPAPTM 484
            LCND+LSG   + D H+LPASHINY DGQ +LSYINS +NPMGYLIPP AKVNTKPAPTM
Sbjct: 1175 LCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTKPAPTM 1234

Query: 485  AAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVSPTGEAFDNRTVPFITMSGTSMSCP 544
            A FSSRGPN ISPEIIKPDVTAPGVN+IAAFSEA+SPT +A DNRT PFITMSGTSMSCP
Sbjct: 1235 AVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGTSMSCP 1294

Query: 545  HVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTMKPMLDGGSPDLAPATPFAYGSGHI 604
            HV+GLVGLLR LHP WSPSAIKSAIMTS+++RDNT+ PM+DGGS DLAPATPFAYGSGHI
Sbjct: 1295 HVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHI 1354

Query: 605  RPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAFSDGPFKCPPNASILNFNYPSIGVQ 664
             PTGAIDPGLVYDLSPNDYLEFLCASGYD+KTIRAFSD PFKCPP +S+LN NYPSIGVQ
Sbjct: 1355 NPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYPSIGVQ 1414

Query: 665  NLNGNVTVTRKLKNVSTPGVYRARIMNPDGVKVSVKPKVLKFERVAEEKSFELTMTGNVP 724
            NL G+V+VTRKLKNV +PGVYRA+I++P+GV VSVKP+ LKFERV EEKSFELT+ G VP
Sbjct: 1415 NLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLAGVVP 1474

Query: 725  EDQVVDGVLIWTDGKHFVRSPIVISSVLF 753
            +D+V  G LIW+DG+H VRSPIV+SS LF
Sbjct: 1475 KDRVGYGALIWSDGRHVVRSPIVVSSGLF 1503

BLAST of Bhi08G000585 vs. ExPASy TrEMBL
Match: A0A6J1EGQ8 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434031 PE=3 SV=1)

HSP 1 Score: 1236.5 bits (3198), Expect = 0.0e+00
Identity = 606/751 (80.69%), Postives = 664/751 (88.42%), Query Frame = 0

Query: 4   QSYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFLGSEEKAKDAIFYSYKKHINGFAAT 63
           +SY+VLLGSH+HG+E++  DL+RVV SHHKLLGSFLGS EKAKDAIFYSYK HINGFAA 
Sbjct: 3   ESYIVLLGSHAHGLEVSAVDLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAI 62

Query: 64  LDEEEATKLAEHPEVAAILANRAKALHTTHSWEFMHLEKNGVIPSSSPWRIAKSGKDVII 123
           LDEEEATKLA+HPE+AA+L N+AK LHTT SWEFMHLEKNGV P SSPWR A+SG++VII
Sbjct: 63  LDEEEATKLAKHPEIAAVLPNKAKELHTTRSWEFMHLEKNGVSPPSSPWRKARSGQNVII 122

Query: 124 ANLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETPDRVPCNRKLIGAKYFNKGFIAYLK 183
           ANLDTGVWPESKSFGEHGIVG VPSKWKGGCMD+TPD VPCN+KLIGAKYFN G IAYLK
Sbjct: 123 ANLDTGVWPESKSFGEHGIVGGVPSKWKGGCMDKTPDAVPCNKKLIGAKYFNHGVIAYLK 182

Query: 184 SENST-EFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVFGSGAGTAKGGSPKARVAAYK 243
           SEN T E S ++NSTRDYEGHGSHTLSTAGGSYVSG SVFGSG GTAKGGSPKA VAAYK
Sbjct: 183 SENLTKELSFIVNSTRDYEGHGSHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKAHVAAYK 242

Query: 244 VCWPL-EGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSDPAEYSEDSIAIAAFHAVKKGIP 303
           VCWPL + GGCFD+DI +AFDHAIHDGVDVLS+SLGS PAEY  D IAIA+FHA+KKGIP
Sbjct: 243 VCWPLHDTGGCFDADIAKAFDHAIHDGVDVLSVSLGSPPAEYYNDIIAIASFHALKKGIP 302

Query: 304 VVCSAGNSGPDALTASNTAPWILTVGASTLDREFQAPVELQNGHRYMGSSLSKALQGNKL 363
           VVCSAGN GP   T SNTAPWILTVGASTLDREFQAP+EL+NG  + GSSLS  L G KL
Sbjct: 303 VVCSAGNDGPSMATVSNTAPWILTVGASTLDREFQAPIELRNGKHFKGSSLSGPLSGRKL 362

Query: 364 YPLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILACLRGDNARVDKGEQAALAGAVG 423
           YPLITGAQAKA  A+ DDAMLCKP+TLDHSKVKGKIL CLRGD++RVDKG QA LAGAVG
Sbjct: 363 YPLITGAQAKATTASTDDAMLCKPKTLDHSKVKGKILVCLRGDSSRVDKGVQALLAGAVG 422

Query: 424 MILCNDELSGFETIADPHVLPASHINYNDGQAVLSYINSTKNPMGYLIPPSAKVNTKPAP 483
           MILCND LSGFE IADPHVLPASHI+YNDGQAV SYINSTKNPMG+LIPP +KVNTKP+P
Sbjct: 423 MILCNDRLSGFEIIADPHVLPASHISYNDGQAVSSYINSTKNPMGHLIPPLSKVNTKPSP 482

Query: 484 TMAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVSPTGEAFDNRTVPFITMSGTSMS 543
           TMAAFSSRGPN++SPEIIKPDVTAPGVN+IAAFS +VSPTGE FDNRTVP+ITMSGTSMS
Sbjct: 483 TMAAFSSRGPNMVSPEIIKPDVTAPGVNIIAAFSGSVSPTGEPFDNRTVPYITMSGTSMS 542

Query: 544 CPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTMKPMLDGGSPDLAPATPFAYGSG 603
           CPHVSG+VGL++ LHP WSP+AIKSAIMTSARIRDNTM  MLDGGSP  A AT F +GSG
Sbjct: 543 CPHVSGIVGLVKALHPKWSPAAIKSAIMTSARIRDNTMNIMLDGGSPIFASATAFIFGSG 602

Query: 604 HIRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAFSDGPFKCPPNASILNFNYPSIG 663
           HIRPTGAIDPGLVYDLSPNDYLEFLCASGY +K +R F+DG FKCP ++SILNFNYPSIG
Sbjct: 603 HIRPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNLRVFADGNFKCPVSSSILNFNYPSIG 662

Query: 664 VQNLNGNVTVTRKLKNVSTPGVYRARIMNPDGVKVSVKPKVLKFERVAEEKSFELTMTGN 723
           VQNL GNVT+TR+LKNV  PGVYR R+  P+GVKVSVKP VLKFE++ EEK FELTMTG 
Sbjct: 663 VQNLTGNVTLTRRLKNVGRPGVYRVRVQQPEGVKVSVKPSVLKFEKIGEEKRFELTMTGV 722

Query: 724 VPEDQVVDGVLIWTDGKHFVRSPIVISSVLF 753
           V   Q+  G LIWTDGKH VRSPIV+SS  F
Sbjct: 723 VANGQIGYGTLIWTDGKHLVRSPIVVSSGFF 753

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G59810.16.8e-25959.44Subtilase family protein [more]
AT2G04160.12.6e-23454.80Subtilisin-like serine endopeptidase family protein [more]
AT5G67360.12.7e-16246.26Subtilase family protein [more]
AT5G45650.13.9e-15341.05subtilase family protein [more]
AT4G34980.16.6e-15343.38subtilisin-like serine protease 2 [more]
Match NameE-valueIdentityDescription
F4JXC59.6e-25859.44Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... [more]
Q9ZSP53.7e-23354.80Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... [more]
I1N4621.7e-18247.46Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... [more]
O653513.7e-16146.26Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
A9JQS71.9e-15242.53Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LVY80.0e+0087.37Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1[more]
A0A1S3CLB70.0e+0086.78subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=... [more]
A0A6J1CN430.0e+0081.49subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012645 P... [more]
A0A5D3CZ660.0e+0081.98Subtilisin-like protease SBT5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A6J1EGQ80.0e+0080.69subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434031 PE... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 199..212
score: 32.67
coord: 536..552
score: 51.76
coord: 118..137
score: 32.49
NoneNo IPR availableGENE3D2.60.40.2310coord: 626..750
e-value: 4.2E-35
score: 122.3
NoneNo IPR availableGENE3D3.50.30.30coord: 332..476
e-value: 1.3E-179
score: 599.8
NoneNo IPR availablePANTHERPTHR43806:SF40BNAC02G33520D PROTEINcoord: 4..749
NoneNo IPR availablePANTHERPTHR43806PEPTIDASE S8coord: 4..749
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 94..609
score: 28.777288
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 339..468
e-value: 1.04689E-36
score: 132.152
NoneNo IPR availableSUPERFAMILY52025PA domaincoord: 390..461
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 5..85
e-value: 5.6E-14
score: 52.6
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 2..90
e-value: 3.7E-21
score: 77.4
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 118..579
e-value: 1.1E-44
score: 153.3
IPR003137PA domainPFAMPF02225PAcoord: 383..453
e-value: 1.3E-9
score: 38.0
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 120..622
e-value: 1.3E-179
score: 599.8
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 89..613
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 654..747
e-value: 9.8E-26
score: 89.9
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 537..547
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 89..572
e-value: 6.53455E-130
score: 386.183

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M000585Bhi08M000585mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008236 serine-type peptidase activity