Bhi08G000374 (gene) Wax gourd (B227) v1

Overview
NameBhi08G000374
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionMitochondrial import inner membrane translocase, subunit TIM22
Locationchr8: 15476460 .. 15482087 (+)
RNA-Seq ExpressionBhi08G000374
SyntenyBhi08G000374
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAAAAAAAAGAAAGAAAGAAAACCGTGTTTCCCTCCCACTCCCAGCAGGCCCTCACCTTTCTCCAAGTTTCTCTGTCTCGTTCAGATTCCCCGATTTTTTTGCTCTCCCACTCTATCTCGGTTCTTCAACCTTTTGTTTTTCCTTTCATTCCACTTCAATGGCTTCCACTTCTGGAGATAGATCTTCTCTCGATCCACCCACCTCCAATGACTCGGAGAACCCTCCAATTGAGCCTATAAGGATGCCTACACTCGAAGAGATTCGCGGCCAAGATATTTGGAACAACTGTGCAGTTCGCAGCGTTGTTAGTGGAGTCATGGGTAGGTCACCCAATTTTCTTTTTGCTTTTCTTTTGTAAATTCTCCTCGGCATTCTCACTCACGTACTCTGCTCTAGTTTCTCTCGCATTAGCCAAACGAATCTTCATTCTTTTGATCGATTACAACAGTGGTAACTCTGCCACTTTCTCAATATGATGCTTTAATGTTACGTTGAATCGTTCCTATTTTGGTTTTTAAATGAGATAGATTTATGTAGTTTCGGTCTGGGCAGTATATGGATTAAGATCGAGAGGCTTAATCTTTCTTATATAAACATTGTTAGTTTACTGGAACAACCAACTGTATGCAGGTTGTGTATGCTGCCTTAATAATAATATTTGAGTTATTATTAGTTATTTACTTTTCTCCCTGACTAGGAGGTACTTTCTGTTCTGAAATGTATGCAGGCGGTGGGCTTGGATTGTTCATGGGATTATTTTTGGGGGCATTGGATAATCCACTGATGCAAGACGAGATGACTGCTAAACAGCAATTTGTTTATACTGCAAAACAGATGGGACGACGGAGTTGGGGTTCTGCAAAAGCGTTTGCAGTTATGGGCTTTATTTTCTCGGCTGCAGAATGTGTTGTAGAAAAGGTATCAATTTCGTGTCTTGTGCACATGGTTGTTTATTTTATTTACCCCTCTCAGTATGTGCCTGTTATTTTATGGGATCTAATTCCATATCGATGTGCTTAGGCCCGAGCCAAACATGATATGACGAACACAGTAGTTGCTGGTTGTGTAACTGGTGGTTCAATATCAGCTAAAGGTAGGTCTTCTTACCCATCTATGTACTTGCCTTATTTTCCAGACAAGTGTTGCATACGTGTGAATTTTCAGCTAGCCATGTAAAGATCTTTATGGAAACAATCTGATGATTCAGACATTCAGTATTCTGGTTGCAACTAAAATATCTGAAGGGTCGTGCATCTTATATTAAATTGTTTTCAAGAAGAAATTATAGTCACAATCTTACTGAACAAGATATGTTCTATTATCTACATATGCATCCTTGAATTTTAAGAGTACTATCATAACCTCTTGTCTTTTAGGTCACATTACAAGGAAATTTGTAGGTACAGGCAGGTTGTCAATGTGTATGCATAAGTAGTTCAACTGTCTCGCTAATTTGTTGTATCTCCGTAATTTCTGAATCTGATCACATTTTGTTGATATCTCATCTTTTAAGTTGTCAGGGAATAATCTGTTTATTTTTCATGGTTGGCATCAATAACACTGCTGTGTCTGCACTAGCTTGTGCTGATCTTTTTCTTTTCATTTTTTACGATTCGCTAATATGCTTGATAATTCTTTGGTAATTAGTAGCCTTGTTGGTAAATTTTCTATATTTTTAGATTAGAAACAATCTCATTGATGGGTAAATTTTGCAAAAGTAAAAGATTTATGGAGTTTGCTCATAGATGCCCTGAATTTGTTTATATATGGGAGGGGAGTAATTACGAAATTGTTTTGAACAGAATCTGCAAAGAGAAGCAACAAAGGCAGTGATCTCCCAAAGTGTTGGAGGATCCTTGAAAGTTTCTCCTACGTCTGTTTAGCCCAACCTCACTTAGAATAATTTTAGAAGCATTTAGTCAAACCATTCTAGCTATACTTTTACCTTACAGAATTGCCAAGGAGAGTATACTTGGTCGAGAAGGGCATAGCCCAGCTCATCTCAGAAGCACAAAAATTTGTCCACAACAACTGAAAGGTAAAGGACGAGAGAATAAAATGTCTAGCATTCTCAGTATATTCTTGCAAATAAATTGCAATTTGAGAAACAAACCTTTGCAGATTCCTTTCCTGGTCGTGTCCTTTGTTTAAGTCTCCAAGTAAAGTCCAAAGTGAAAGTTTTACCTTCATTGGATATATGTGTCTTTTCCCGAGAAGTTTATCTTCGTTTGTGTAAGCCTCCAAGTGAAATCCAAAGTGAAACATTTACTTTCATTGGATACTTGTCTTTTCTTTTCCTGATTAACCTGTTTTTCTCAATTCTCACTGCTAACATTACAGTTTTTCAGCATCAGACGAAGGATTTTCCATTCCTAAATCTTCCTATCTTTCAAATTTCCATGCAAATTAAAGCACAAAGAGCTTTGTTAGTGATCCCACCCAGACTGCCTTACAGATACAAGTTTACTACTAGTTAGACCATACAGCCTGTGAATGGCATTTGACAGTGGCTGGTCCATGATTCAAATATCTTAAAAAATTTAAAATATTTTCTCTGCCAACACGTTTAAGGAAATCTCAACTTCTACAAAAACAATGATGGATAAAACTTGGGGGGAAAGACGAGACCATACAAGAACATAGGCGAAACACCCAACAAAAGAACCACCTAAAAATTACCACAAGAAGGGTCGGGGGGTTGATAGGTTGGTTCAGAGTTGCTTCCTCTTTGACTGGTAGTCGGTGTCCAATTTTTTTGCATGCCATTCTTGCACTGGCTCATACAGCCAAAGACACATACATTTTTAATGTGGAGAAAATTATAGGACAGGACAAGAAGAGTTTTGGTAAAGCTTTAGGAGCAATCTATGAAAGTTCTCTTTAGCATGATCTATGCACGCCATCTGATTATCACTTGGGCAATATTGTATAGTACTTGTTGACTTAACTTGCACGGTCATGCTCTTGTTGAACCTTTATAGTATTCCCTGATTTTAAAAGATTTGAAGTAGAAGGCAAGCAACTGCCATCTAACGCTCCTTGGCTCCTCCACTGTTAGCAAGAGCAAAGCTAAATTTATTGAAGAGCTATTTTAGTTTGAAGTTATTAATGTTGCTTGCCTTGGTATTTTAGACTAGACAATGAGGGATGGACCTCCAATGGGTTTTTGGATAATAAGAGATGGTAAAAATTCTATTCGGGTCTCTATACCTTGGATCTCATTTTATTTTAGTCATTATACTTTCAAATGTTCAATTTGTCCCCGTACTTTCATAAATATTAAAATTAGTCCTCATTGTTAGTTTGAAGTTATTAATGTTGTTGTGTGTGTGTTTTTTTTTTTGGGGNNNNNNNNNNNNNNNNNNNNCTTGGTAAAGTAGTAATAATAATTAATTTTATAACGAAAATCATGATGTGAATAATGCTTCTAAAATATGTAGAGGGAAGAATGAAGACTCCCTCCAAGCCTTTCATCTGAACAATAAAACGCTTAGATGAAATGAGTTAAAATCATGGTAGCTACCTACCTAGAATTTAATAGTCGAGACTAGTTTATTTATATATTTTGTAAGTTAACAAAAAAAAAATAAAAAATAGTGAGACTCTTTTTAAGATTTATTGAAAATATAGGGATGAAATTTAGACATTTGAAAGTAAAGAGAGGAAATCTGGTCAGTAGTTCCTGCTTTTAACTTTACATAGTTATGCAACTTAGTGACAATCTCTTTAGCCTCTTACCATTATTTTATCTATTGAGCAATGTTTCAAGAATGAATGATTGCCAGAATAACATAAATGGCTGTGTTAACTGAGATTGTCTTTATTTTAACAACGTTGATTATCGTTTTTAATGCGCTTCTCGTGTTCATCCCAAGATGCCATATCTCATCTTAAGTGGTCTTTATCTCAGGTGGCCCAAAGGCAGCTTGTGCTGGTTGTGCAGGCTTTGCTGCATTCTCAGTCTTGATAGAGAAGTTTTTGGACAGACACAGTTGAGCTGCGTTTGTGCTCACAATTTTCTGATAGTAAAAGCAAACCAATGGTAGAGAAATAATAGTTAGCCTGCAGTGTAACCATTCTTTTCATCATAAAAATATGAACTTTCTTTTTTATTTGGGATGACTTTAGAATATTGTCCCCATTGTTTTAGTAACATTATCCGCAATCCCATTTTGTTTGGAAGATATTTATATTCATAGAAAATTAGGCCATTTGGTTTGAATCTCATGTCATAAGTTATCAAAAATATTCGTGAACTATTGAGAGTTGGTTTCGATTATATCCTAAAATTTTGAAAATTTCCATTTAAACATTTGAATAAAGATTTTTTTCCCAACAACTTTTGAAGGCTTCTGCCGTTAAATTTATTGACTAAATTGATTTTGTTTATTTATTTGTTGCTTAAACGAATAAATCTAATCACATAGGAGAAGTATATGTGAGGTTTAGAGCCAGAGGTATCCAAAAAACCATAAACAAAGGAACAAAAATGGGAGACTGCGGGCTTATTGGATGAAGATTTACAACGAGCATGGTTCACATAAGCACTGTCCTCTTTTTTTTTGTTCGTTTTTTTTGGTTGGTTTTTTTTTAAAATTAATGTATGTACGTGTTTAGATTTGTTTTCATAAGCATATATACGTTTTTTTGCTAATAATTTATAAGTAAAATGAAATTTTTAATGGTTATAATTGAAACCAACTCATAATATAAGTTAATGATAGATATAACAACACATGAGACTCTAATAAAGACTATATTTCAATTACGTTCAACTTTTTCATTATTTACAAGAATATTTAAGTGGAATTTTTAACTACACAAATATTAGGTTCGAATTACTTTTTTGTCCTCAATCTATTACACCTACACCATGTTTACTTTGGTGGATGAAAAAGGGTGTAACCTGGGGTGATTCCAATTGGACCATCAATTGGAATGACAGGTAAAATGATTTTAGAGATTTCTACATAAAAGACCTAAAGAATAGATACATATTATGTTAATATCTTGAACTATTTCATTTTTTTTTCAAAAAAAAAAAAATCTATTGGCCTCTCTATTTTCACTAATACCAGAATTTAGCCCACACACACACACACATATATATATATATATATTTATTTTTCTCTCCTCCATGCATACTCTCTCTCCCCCTGCATATATTCTCTATCCCCAATAATATATTTCATATTCTCTCCCCATAATTCACATCAATTTTCCCCCTCCCTCTCTTTTCTCCTTCCAGCGGTTGAGATGCAAGCGGTAGACGATTGCGTGGGGCGAATTGATGGCTCAACACGAACAACTTGGGACGAATCGATGGCGCGGACAACATGGGGCAAATCGATGGTGACGGGATGAGCAGATTCGATCGACGGCACGACGGGGCGACCAAATCTGATTGACGACGATGAGGAGAGAGATTCGTGGCGGCCATGGCTAAAGAAGAAGAAGAGATGAAGATGGAGAGTTATATATTATAAACTCCCATATGAAGATGAACTTTATTTTTTCCTATTTTTTACATATAATGTGATATATATGTATTTTTTTTTAGCTTTTGTCGGAAATTTAATATATACTATGGTATATGTATTCATTTATTTGATATAAAC

mRNA sequence

AAAAAAAAAAAAAAAAAAGAAAGAAAGAAAACCGTGTTTCCCTCCCACTCCCAGCAGGCCCTCACCTTTCTCCAAGTTTCTCTGTCTCGTTCAGATTCCCCGATTTTTTTGCTCTCCCACTCTATCTCGGTTCTTCAACCTTTTGTTTTTCCTTTCATTCCACTTCAATGGCTTCCACTTCTGGAGATAGATCTTCTCTCGATCCACCCACCTCCAATGACTCGGAGAACCCTCCAATTGAGCCTATAAGGATGCCTACACTCGAAGAGATTCGCGGCCAAGATATTTGGAACAACTGTGCAGTTCGCAGCGTTGTTAGTGGAGTCATGGGCGGTGGGCTTGGATTGTTCATGGGATTATTTTTGGGGGCATTGGATAATCCACTGATGCAAGACGAGATGACTGCTAAACAGCAATTTGTTTATACTGCAAAACAGATGGGACGACGGAGTTGGGGTTCTGCAAAAGCGTTTGCAGTTATGGGCTTTATTTTCTCGGCTGCAGAATGTGTTGTAGAAAAGGCCCGAGCCAAACATGATATGACGAACACAGTAGTTGCTGGTTGTGTAACTGGTGGTTCAATATCAGCTAAAGGTGGCCCAAAGGCAGCTTGTGCTGGTTGTGCAGGCTTTGCTGCATTCTCAGTCTTGATAGAGAAGTTTTTGGACAGACACAGTTGAGCTGCGTTTGTGCTCACAATTTTCTGATAGTAAAAGCAAACCAATGCGGTTGAGATGCAAGCGGTAGACGATTGCGTGGGGCGAATTGATGGCTCAACACGAACAACTTGGGACGAATCGATGGCGCGGACAACATGGGGCAAATCGATGGTGACGGGATGAGCAGATTCGATCGACGGCACGACGGGGCGACCAAATCTGATTGACGACGATGAGGAGAGAGATTCGTGGCGGCCATGGCTAAAGAAGAAGAAGAGATGAAGATGGAGAGTTATATATTATAAACTCCCATATGAAGATGAACTTTATTTTTTCCTATTTTTTACATATAATGTGATATATATGTATTTTTTTTTAGCTTTTGTCGGAAATTTAATATATACTATGGTATATGTATTCATTTATTTGATATAAAC

Coding sequence (CDS)

ATGGCTTCCACTTCTGGAGATAGATCTTCTCTCGATCCACCCACCTCCAATGACTCGGAGAACCCTCCAATTGAGCCTATAAGGATGCCTACACTCGAAGAGATTCGCGGCCAAGATATTTGGAACAACTGTGCAGTTCGCAGCGTTGTTAGTGGAGTCATGGGCGGTGGGCTTGGATTGTTCATGGGATTATTTTTGGGGGCATTGGATAATCCACTGATGCAAGACGAGATGACTGCTAAACAGCAATTTGTTTATACTGCAAAACAGATGGGACGACGGAGTTGGGGTTCTGCAAAAGCGTTTGCAGTTATGGGCTTTATTTTCTCGGCTGCAGAATGTGTTGTAGAAAAGGCCCGAGCCAAACATGATATGACGAACACAGTAGTTGCTGGTTGTGTAACTGGTGGTTCAATATCAGCTAAAGGTGGCCCAAAGGCAGCTTGTGCTGGTTGTGCAGGCTTTGCTGCATTCTCAGTCTTGATAGAGAAGTTTTTGGACAGACACAGTTGA

Protein sequence

MASTSGDRSSLDPPTSNDSENPPIEPIRMPTLEEIRGQDIWNNCAVRSVVSGVMGGGLGLFMGLFLGALDNPLMQDEMTAKQQFVYTAKQMGRRSWGSAKAFAVMGFIFSAAECVVEKARAKHDMTNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVLIEKFLDRHS
Homology
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BLAST of Bhi08G000374 vs. TAIR 10
Match: AT3G10110.1 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 254.2 bits (648), Expect = 7.3e-68
Identity = 119/158 (75.32%), Postives = 137/158 (86.71%), Query Frame = 0 

Query: 13  PPTSNDSENPPIEPIRMPTLEEIRGQDIWNNCAVRSVVSGVMGGGLGLFMGLFLGALDNP 72
           PP ++D  +  I+PIRMPT+EEIR Q++WNNCAVR+V SGVMGGGLGL MGLFLGALDNP
Sbjct: 16  PPVASDENSTQIQPIRMPTIEEIRAQEVWNNCAVRAVTSGVMGGGLGLMMGLFLGALDNP 75

Query: 73  LMQDEMTAKQQFVYTAKQMGRRSWGSAKAFAVMGFIFSAAECVVEKARAKHDMTNTVVAG 132
           +  D MTA+QQFV+TAKQMG+RSW S K FAVMG +FSAAEC+VEKARAKHD  NT +AG
Sbjct: 76  ITHDTMTARQQFVFTAKQMGQRSWNSCKTFAVMGLVFSAAECIVEKARAKHDTVNTAIAG 135

Query: 133 CVTGGSISAKGGPKAACAGCAGFAAFSVLIEKFLDRHS 171
           CVTGGS+SA+GGPKAAC GCAGFA FSVLIEKF DRH+
Sbjct: 136 CVTGGSMSARGGPKAACIGCAGFATFSVLIEKFFDRHT 173

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BLAST of Bhi08G000374 vs. TAIR 10
Match: AT1G18320.1 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 239.6 bits (610), Expect = 1.9e-63
Identity = 112/142 (78.87%), Postives = 126/142 (88.73%), Query Frame = 0 

Query: 29  MPTLEEIRGQDIWNNCAVRSVVSGVMGGGLGLFMGLFLGALDNPLMQDEMTAKQQFVYTA 88
           MPT+EEIR Q++WNNCAVR+V SGVMGGGLGL MGLFLGALDNP+  D MTA+QQFV+TA
Sbjct: 1   MPTIEEIRAQEVWNNCAVRAVTSGVMGGGLGLMMGLFLGALDNPITHDTMTARQQFVFTA 60

Query: 89  KQMGRRSWGSAKAFAVMGFIFSAAECVVEKARAKHDMTNTVVAGCVTGGSISAKGGPKAA 148
           KQMG+RSW S K FAVMG +FSAAEC+VEKARAKHD  NT +AGCVTGGS+SA+GGPKAA
Sbjct: 61  KQMGQRSWNSCKTFAVMGLVFSAAECIVEKARAKHDTVNTAIAGCVTGGSMSARGGPKAA 120

Query: 149 CAGCAGFAAFSVLIEKFLDRHS 171
           C GCAGFA FSVLIEKF DRH+
Sbjct: 121 CIGCAGFAIFSVLIEKFFDRHT 142

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BLAST of Bhi08G000374 vs. ExPASy Swiss-Prot
Match: A2RVP7 (Mitochondrial import inner membrane translocase subunit TIM22-1 OS=Arabidopsis thaliana OX=3702 GN=TIM22-1 PE=1 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 1.0e-66
Identity = 119/158 (75.32%), Postives = 137/158 (86.71%), Query Frame = 0 

Query: 13  PPTSNDSENPPIEPIRMPTLEEIRGQDIWNNCAVRSVVSGVMGGGLGLFMGLFLGALDNP 72
           PP ++D  +  I+PIRMPT+EEIR Q++WNNCAVR+V SGVMGGGLGL MGLFLGALDNP
Sbjct: 16  PPVASDENSTQIQPIRMPTIEEIRAQEVWNNCAVRAVTSGVMGGGLGLMMGLFLGALDNP 75

Query: 73  LMQDEMTAKQQFVYTAKQMGRRSWGSAKAFAVMGFIFSAAECVVEKARAKHDMTNTVVAG 132
           +  D MTA+QQFV+TAKQMG+RSW S K FAVMG +FSAAEC+VEKARAKHD  NT +AG
Sbjct: 76  ITHDTMTARQQFVFTAKQMGQRSWNSCKTFAVMGLVFSAAECIVEKARAKHDTVNTAIAG 135

Query: 133 CVTGGSISAKGGPKAACAGCAGFAAFSVLIEKFLDRHS 171
           CVTGGS+SA+GGPKAAC GCAGFA FSVLIEKF DRH+
Sbjct: 136 CVTGGSMSARGGPKAACIGCAGFATFSVLIEKFFDRHT 173

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BLAST of Bhi08G000374 vs. ExPASy Swiss-Prot
Match: A1XJK0 (Mitochondrial import inner membrane translocase subunit TIM22-4 OS=Arabidopsis thaliana OX=3702 GN=TIM22-4 PE=2 SV=3)

HSP 1 Score: 239.6 bits (610), Expect = 2.6e-62
Identity = 112/142 (78.87%), Postives = 126/142 (88.73%), Query Frame = 0 

Query: 29  MPTLEEIRGQDIWNNCAVRSVVSGVMGGGLGLFMGLFLGALDNPLMQDEMTAKQQFVYTA 88
           MPT+EEIR Q++WNNCAVR+V SGVMGGGLGL MGLFLGALDNP+  D MTA+QQFV+TA
Sbjct: 1   MPTIEEIRAQEVWNNCAVRAVTSGVMGGGLGLMMGLFLGALDNPITHDTMTARQQFVFTA 60

Query: 89  KQMGRRSWGSAKAFAVMGFIFSAAECVVEKARAKHDMTNTVVAGCVTGGSISAKGGPKAA 148
           KQMG+RSW S K FAVMG +FSAAEC+VEKARAKHD  NT +AGCVTGGS+SA+GGPKAA
Sbjct: 61  KQMGQRSWNSCKTFAVMGLVFSAAECIVEKARAKHDTVNTAIAGCVTGGSMSARGGPKAA 120

Query: 149 CAGCAGFAAFSVLIEKFLDRHS 171
           C GCAGFA FSVLIEKF DRH+
Sbjct: 121 CIGCAGFAIFSVLIEKFFDRHT 142

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BLAST of Bhi08G000374 vs. ExPASy Swiss-Prot
Match: Q2UAP8 (Mitochondrial import inner membrane translocase subunit tim22 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=tim22 PE=3 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 7.5e-25
Identity = 59/131 (45.04%), Postives = 87/131 (66.41%), Query Frame = 0 

Query: 43  NCAVRSVVSGVMGGGLGLFMGLFLGAL--DNPLMQD-----EMTAKQQFVYTAKQMGRRS 102
           +C V++V+SG MG GLG   GLF+ ++  D+          ++  ++Q     K MG RS
Sbjct: 48  SCPVKTVISGTMGFGLGGVFGLFMASMSYDSTFTPQGKAIMDLPWREQVRRGFKDMGSRS 107

Query: 103 WGSAKAFAVMGFIFSAAECVVEKARAKHDMTNTVVAGCVTGGSISAKGGPKAACAGCAGF 162
           W SAK F ++G ++S  EC VE  RAK+D++N+V++GC+TGG + AK GP+AA AGCAGF
Sbjct: 108 WSSAKNFGIVGALYSGTECCVEGLRAKNDLSNSVISGCITGGILGAKAGPQAAAAGCAGF 167

Query: 163 AAFSVLIEKFL 167
           AAFS  I+ ++
Sbjct: 168 AAFSAAIDAYM 178

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BLAST of Bhi08G000374 vs. ExPASy Swiss-Prot
Match: A0A1D8PI78 (Mitochondrial import inner membrane translocase subunit TIM22 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=TIM22 PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.7e-24
Identity = 65/160 (40.62%), Postives = 93/160 (58.13%), Query Frame = 0 

Query: 22  PPIEPIRMPTLEEIRGQ------DIWNNCAVRSVVSGVMGGGLGLFMGLFLG--ALDNPL 81
           PP +P++  T EE   +         N+C  ++V++GV G  LG F GLF+   A D P+
Sbjct: 12  PPKKPLQEMTQEEQAEEGARQMIGFMNSCPGKTVMAGVSGFALGGFFGLFMASMAYDTPI 71

Query: 82  MQD------EMTAKQQFVYTAKQMGRRSWGSAKAFAVMGFIFSAAECVVEKARAKHDMTN 141
             D      E+  KQQ       M +RS+ SAK F  +G ++S  EC +E  RAKHD+ N
Sbjct: 72  GTDAVKHISELPFKQQMKLQFTDMAKRSYSSAKNFGYIGMVYSGVECTIESLRAKHDIYN 131

Query: 142 TVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVLIEKFLD 168
            V AGC+TG  ++ + GP+AA  GCAGFAAFS+ I+ +L+
Sbjct: 132 GVSAGCITGAGLAIRAGPQAALVGCAGFAAFSLAIDMYLN 171

[Close]
BLAST of Bhi08G000374 vs. ExPASy Swiss-Prot
Match: Q6BZY4 (Mitochondrial import inner membrane translocase subunit TIM22 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=TIM22 PE=3 SV=2)

HSP 1 Score: 109.4 bits (272), Expect = 4.1e-23
Identity = 59/131 (45.04%), Postives = 81/131 (61.83%), Query Frame = 0 

Query: 43  NCAVRSVVSGVMGGGLGLFMGLFLG--ALDNPL-----MQDEMTAKQQFVYTAKQMGRRS 102
           +C  +SV++GV G GLG   GLF+   A D P+        ++  KQQ       MG+R+
Sbjct: 41  SCPGKSVMAGVTGFGLGGVFGLFMASMAYDAPVGMGVQTMSDLPFKQQMKIQFTDMGKRA 100

Query: 103 WGSAKAFAVMGFIFSAAECVVEKARAKHDMTNTVVAGCVTGGSISAKGGPKAACAGCAGF 162
           W SAK F  +G +FS  EC +E  RAK+D+ N V AGC+TGG ++ K GP+AA  GCAGF
Sbjct: 101 WSSAKNFGFIGGVFSGTECCIESLRAKNDIWNGVAAGCLTGGGLAVKAGPQAALVGCAGF 160

Query: 163 AAFSVLIEKFL 167
           AAFS  I+ ++
Sbjct: 161 AAFSAAIDVYM 171

[Close]
BLAST of Bhi08G000374 vs. ExPASy TrEMBL
Match: A0A6J1K8G7 (mitochondrial import inner membrane translocase subunit TIM22-4 OS=Cucurbita maxima OX=3661 GN=LOC111493200 PE=4 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 4.5e-81
Identity = 154/170 (90.59%), Postives = 165/170 (97.06%), Query Frame = 0 

Query: 1   MASTSGDRSSLDPPTSNDSENPPIEPIRMPTLEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60
           MAS+SGD SS DP +S++ E PP+EPIRMPT+EEIRGQDIWNNCAVRSVVSGVMGGGLGL
Sbjct: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60

Query: 61  FMGLFLGALDNPLMQDEMTAKQQFVYTAKQMGRRSWGSAKAFAVMGFIFSAAECVVEKAR 120
           FMGLFLGALDNPLMQDEMTA+QQFVYTAKQMGRRSWGSAKAFAVMGF+FSAAECVVEKAR
Sbjct: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKAR 120

Query: 121 AKHDMTNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVLIEKFLDRHS 171
           AKHD+TNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSV+IEKFLDRH+
Sbjct: 121 AKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 170

[Close]
BLAST of Bhi08G000374 vs. ExPASy TrEMBL
Match: A0A6J1G8S5 (mitochondrial import inner membrane translocase subunit TIM22-4 OS=Cucurbita moschata OX=3662 GN=LOC111452007 PE=4 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 4.5e-81
Identity = 154/170 (90.59%), Postives = 165/170 (97.06%), Query Frame = 0 

Query: 1   MASTSGDRSSLDPPTSNDSENPPIEPIRMPTLEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60
           MAS+SGD SS DP +S++ E PP+EPIRMPT+EEIRGQDIWNNCAVRSVVSGVMGGGLGL
Sbjct: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60

Query: 61  FMGLFLGALDNPLMQDEMTAKQQFVYTAKQMGRRSWGSAKAFAVMGFIFSAAECVVEKAR 120
           FMGLFLGALDNPLMQDEMTA+QQFVYTAKQMGRRSWGSAKAFAVMGF+FSAAECVVEKAR
Sbjct: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKAR 120

Query: 121 AKHDMTNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVLIEKFLDRHS 171
           AKHD+TNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSV+IEKFLDRH+
Sbjct: 121 AKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 170

[Close]
BLAST of Bhi08G000374 vs. ExPASy TrEMBL
Match: A0A6J1DKY7 (mitochondrial import inner membrane translocase subunit TIM22-4-like OS=Momordica charantia OX=3673 GN=LOC111022051 PE=4 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.0e-80
Identity = 154/170 (90.59%), Postives = 164/170 (96.47%), Query Frame = 0 

Query: 1   MASTSGDRSSLDPPTSNDSENPPIEPIRMPTLEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60
           MA+ +G+ SS DP +SN++E PPIEPIRMPT+EEIRGQDIWNNCAVRSVVSGVMGGGLGL
Sbjct: 1   MATNAGNGSSHDPTSSNEAEKPPIEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60

Query: 61  FMGLFLGALDNPLMQDEMTAKQQFVYTAKQMGRRSWGSAKAFAVMGFIFSAAECVVEKAR 120
           FMGLFLGALDNPLMQ+EMTAKQQF++TAKQMGRRSWGSAKAFAVMGFIFSAAECVVEKAR
Sbjct: 61  FMGLFLGALDNPLMQEEMTAKQQFIFTAKQMGRRSWGSAKAFAVMGFIFSAAECVVEKAR 120

Query: 121 AKHDMTNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVLIEKFLDRHS 171
           AKHD TNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVLIEKFLDRHS
Sbjct: 121 AKHDTTNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVLIEKFLDRHS 170

[Close]
BLAST of Bhi08G000374 vs. ExPASy TrEMBL
Match: A0A1S3C5B1 (mitochondrial import inner membrane translocase subunit TIM22-4-like OS=Cucumis melo OX=3656 GN=LOC103497039 PE=4 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 1.9e-79
Identity = 150/170 (88.24%), Postives = 163/170 (95.88%), Query Frame = 0 

Query: 1   MASTSGDRSSLDPPTSNDSENPPIEPIRMPTLEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60
           MASTSGD  S DPPTSND+E PPI P+RMPT+EEIRGQDIW+NCAVRSVVSGVMGGG+GL
Sbjct: 1   MASTSGDGFSPDPPTSNDAEQPPIAPMRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGIGL 60

Query: 61  FMGLFLGALDNPLMQDEMTAKQQFVYTAKQMGRRSWGSAKAFAVMGFIFSAAECVVEKAR 120
           F+GLFLG++DN LMQDEMTA+QQFVYTAKQMGRR WGSAK+FAVMGFIFSAAECVVEKAR
Sbjct: 61  FLGLFLGSMDNSLMQDEMTARQQFVYTAKQMGRRGWGSAKSFAVMGFIFSAAECVVEKAR 120

Query: 121 AKHDMTNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVLIEKFLDRHS 171
           AKHDMTNTVVAGCVTGG+ISAKGGPKAACAGCAGFAAFSVLIEKFL+RH+
Sbjct: 121 AKHDMTNTVVAGCVTGGAISAKGGPKAACAGCAGFAAFSVLIEKFLERHT 170

[Close]
BLAST of Bhi08G000374 vs. ExPASy TrEMBL
Match: A0A0A0M0Y0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G533580 PE=4 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 5.7e-76
Identity = 144/170 (84.71%), Postives = 160/170 (94.12%), Query Frame = 0 

Query: 1   MASTSGDRSSLDPPTSNDSENPPIEPIRMPTLEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60
           MASTSGD SSLDPPTS+D++ PPI+P+RMPT+EEIRGQDIW+NCAVRSVVSGVMGGGLG 
Sbjct: 1   MASTSGDGSSLDPPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGF 60

Query: 61  FMGLFLGALDNPLMQDEMTAKQQFVYTAKQMGRRSWGSAKAFAVMGFIFSAAECVVEKAR 120
           F+GLFL + D  ++QDEMTA+QQFV TAKQMGRRSWGSAK+FAVMG IFSAAECVVEKAR
Sbjct: 61  FVGLFLASTDTSMVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKAR 120

Query: 121 AKHDMTNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVLIEKFLDRHS 171
           AKHDMTNT+VAGCVTGGSISAKGGPKAACAGCAGFAAFS LIEKFL+RH+
Sbjct: 121 AKHDMTNTIVAGCVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT 170

The following BLAST results are available for this feature:
BLAST of Bhi08G000374 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G10110.17.3e-6875.32Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family... [more]
AT1G18320.11.9e-6378.87Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family... [more]
BLAST of Bhi08G000374 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
A2RVP71.0e-6675.32Mitochondrial import inner membrane translocase subunit TIM22-1 OS=Arabidopsis t... [more]
A1XJK02.6e-6278.87Mitochondrial import inner membrane translocase subunit TIM22-4 OS=Arabidopsis t... [more]
Q2UAP87.5e-2545.04Mitochondrial import inner membrane translocase subunit tim22 OS=Aspergillus ory... [more]
A0A1D8PI781.7e-2440.63Mitochondrial import inner membrane translocase subunit TIM22 OS=Candida albican... [more]
Q6BZY44.1e-2345.04Mitochondrial import inner membrane translocase subunit TIM22 OS=Yarrowia lipoly... [more]
BLAST of Bhi08G000374 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1K8G74.5e-8190.59mitochondrial import inner membrane translocase subunit TIM22-4 OS=Cucurbita max... [more]
A0A6J1G8S54.5e-8190.59mitochondrial import inner membrane translocase subunit TIM22-4 OS=Cucurbita mos... [more]
A0A6J1DKY71.0e-8090.59mitochondrial import inner membrane translocase subunit TIM22-4-like OS=Momordic... [more]
A0A1S3C5B11.9e-7988.24mitochondrial import inner membrane translocase subunit TIM22-4-like OS=Cucumis ... [more]
A0A0A0M0Y05.7e-7684.71Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G533580 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF02466Tim17coord: 44..159
e-value: 2.7E-30
score: 105.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR14110:SF0MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22coord: 17..169
IPR039175Mitochondrial import inner membrane translocase subunit TIM22PANTHERPTHR14110MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22coord: 17..169

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M000374Bhi08M000374mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009793 embryo development ending in seed dormancy
biological_process GO:0045039 protein insertion into mitochondrial inner membrane
biological_process GO:0071806 protein transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0042721 TIM22 mitochondrial import inner membrane insertion complex
molecular_function GO:0030943 mitochondrion targeting sequence binding
molecular_function GO:0008320 protein transmembrane transporter activity