Bhi08G000254 (gene) Wax gourd (B227) v1

Overview
NameBhi08G000254
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionaquaporin PIP2-1-like
Locationchr8: 10724684 .. 10725139 (-)
RNA-Seq ExpressionBhi08G000254
SyntenyBhi08G000254
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGGCTCAGAGTTTGGGTGCCATTTGTGGCTGTGCCTTGGTGAAGGCATTCCAAAACGCTCACTACAATAAGTACGGTGGTCGAGCCAACTCGCTCGCCGACGACTACAACATCGGCACCGGTTTAGCTACCGAGATCATCGGTACATTCGTTCTCGTTTACACTATTTTTTCTCCTACCTATCCTAAGAGAAATGCTAGACTCCCATGTCCCCGTAAGTTCAATATCCATACTATTCAATATTAATGAGATCGAAAATGACATTACAAATAGAATTAATGTTTTTTTTAATATTTGAAATGAAAGGTTTTGGCACCACTCCCAATTGGGTTTGCGGTGTTCATGGTTCACTTGGCCACGATTCCGATCACCGGCACCGGCATCAACCCGGCTAGAAGCTTTGGAGCTGCAGTTGTGTTCAACAAATCCAAGCCATGGGATGATCAAGTATAA

mRNA sequence

ATGACGGCTCAGAGTTTGGGTGCCATTTGTGGCTGTGCCTTGGTGAAGGCATTCCAAAACGCTCACTACAATAAGTACGGTGGTCGAGCCAACTCGCTCGCCGACGACTACAACATCGGCACCGGTTTAGCTACCGAGATCATCGGTACATTCGTTCTCGTTTTGGCACCACTCCCAATTGGGTTTGCGGTGTTCATGGTTCACTTGGCCACGATTCCGATCACCGGCACCGGCATCAACCCGGCTAGAAGCTTTGGAGCTGCAGTTGTGTTCAACAAATCCAAGCCATGGGATGATCAAGTATAA

Coding sequence (CDS)

ATGACGGCTCAGAGTTTGGGTGCCATTTGTGGCTGTGCCTTGGTGAAGGCATTCCAAAACGCTCACTACAATAAGTACGGTGGTCGAGCCAACTCGCTCGCCGACGACTACAACATCGGCACCGGTTTAGCTACCGAGATCATCGGTACATTCGTTCTCGTTTTGGCACCACTCCCAATTGGGTTTGCGGTGTTCATGGTTCACTTGGCCACGATTCCGATCACCGGCACCGGCATCAACCCGGCTAGAAGCTTTGGAGCTGCAGTTGTGTTCAACAAATCCAAGCCATGGGATGATCAAGTATAA

Protein sequence

MTAQSLGAICGCALVKAFQNAHYNKYGGRANSLADDYNIGTGLATEIIGTFVLVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDDQV
Homology
BLAST of Bhi08G000254 vs. TAIR 10
Match: AT2G37170.1 (plasma membrane intrinsic protein 2 )

HSP 1 Score: 159.5 bits (402), Expect = 1.5e-39
Identity = 83/119 (69.75%), Postives = 90/119 (75.63%), Query Frame = 0

Query: 1   MTAQSLGAICGCALVKAFQNAHYNKYGGRANSLADDYNIGTGLATEIIGTFVL------- 60
           M AQ LGAICG   VKAFQ+++Y++YGG ANSLAD YN GTGLA EIIGTFVL       
Sbjct: 127 MVAQCLGAICGVGFVKAFQSSYYDRYGGGANSLADGYNTGTGLAAEIIGTFVLVYTVFSA 186

Query: 61  -------------VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDD 100
                        VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAV++NKSKPWDD
Sbjct: 187 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKSKPWDD 245

BLAST of Bhi08G000254 vs. TAIR 10
Match: AT2G37180.1 (Aquaporin-like superfamily protein )

HSP 1 Score: 158.3 bits (399), Expect = 3.2e-39
Identity = 84/119 (70.59%), Postives = 87/119 (73.11%), Query Frame = 0

Query: 1   MTAQSLGAICGCALVKAFQNAHYNKYGGRANSLADDYNIGTGLATEIIGTFVL------- 60
           M AQ LGAICG   VKAFQ++HY  YGG AN LAD YN GTGLA EIIGTFVL       
Sbjct: 127 MVAQCLGAICGVGFVKAFQSSHYVNYGGGANFLADGYNTGTGLAAEIIGTFVLVYTVFSA 186

Query: 61  -------------VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDD 100
                        VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAV+FNKSKPWDD
Sbjct: 187 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKSKPWDD 245

BLAST of Bhi08G000254 vs. TAIR 10
Match: AT3G53420.1 (plasma membrane intrinsic protein 2A )

HSP 1 Score: 155.2 bits (391), Expect = 2.7e-38
Identity = 81/117 (69.23%), Postives = 88/117 (75.21%), Query Frame = 0

Query: 3   AQSLGAICGCALVKAFQNAHYNKYGGRANSLADDYNIGTGLATEIIGTFVL--------- 62
           AQ LGAICG   VKAFQ+++Y +YGG ANSLAD Y+ GTGLA EIIGTFVL         
Sbjct: 131 AQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGTFVLVYTVFSATD 190

Query: 63  -----------VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDD 100
                      VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAV++NKSKPWDD
Sbjct: 191 PKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKSKPWDD 247

BLAST of Bhi08G000254 vs. TAIR 10
Match: AT3G53420.2 (plasma membrane intrinsic protein 2A )

HSP 1 Score: 155.2 bits (391), Expect = 2.7e-38
Identity = 81/117 (69.23%), Postives = 88/117 (75.21%), Query Frame = 0

Query: 3   AQSLGAICGCALVKAFQNAHYNKYGGRANSLADDYNIGTGLATEIIGTFVL--------- 62
           AQ LGAICG   VKAFQ+++Y +YGG ANSLAD Y+ GTGLA EIIGTFVL         
Sbjct: 131 AQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGTFVLVYTVFSATD 190

Query: 63  -----------VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDD 100
                      VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAV++NKSKPWDD
Sbjct: 191 PKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKSKPWDD 247

BLAST of Bhi08G000254 vs. TAIR 10
Match: AT5G60660.1 (plasma membrane intrinsic protein 2;4 )

HSP 1 Score: 153.3 bits (386), Expect = 1.0e-37
Identity = 79/120 (65.83%), Postives = 86/120 (71.67%), Query Frame = 0

Query: 1   MTAQSLGAICGCALVKAFQNAHYNKYGGRANSLADDYNIGTGLATEIIGTFVL------- 60
           + AQ LGAICGC  VKAFQ+++Y +YGG AN LAD YN GTGL  EIIGTFVL       
Sbjct: 129 IVAQCLGAICGCGFVKAFQSSYYTRYGGGANELADGYNKGTGLGAEIIGTFVLVYTVFSA 188

Query: 61  -------------VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDDQ 101
                        VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAV++N  K WDDQ
Sbjct: 189 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNEKAWDDQ 248

BLAST of Bhi08G000254 vs. ExPASy Swiss-Prot
Match: P43287 (Aquaporin PIP2-2 OS=Arabidopsis thaliana OX=3702 GN=PIP2-2 PE=1 SV=2)

HSP 1 Score: 159.5 bits (402), Expect = 2.1e-38
Identity = 83/119 (69.75%), Postives = 90/119 (75.63%), Query Frame = 0

Query: 1   MTAQSLGAICGCALVKAFQNAHYNKYGGRANSLADDYNIGTGLATEIIGTFVL------- 60
           M AQ LGAICG   VKAFQ+++Y++YGG ANSLAD YN GTGLA EIIGTFVL       
Sbjct: 127 MVAQCLGAICGVGFVKAFQSSYYDRYGGGANSLADGYNTGTGLAAEIIGTFVLVYTVFSA 186

Query: 61  -------------VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDD 100
                        VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAV++NKSKPWDD
Sbjct: 187 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKSKPWDD 245

BLAST of Bhi08G000254 vs. ExPASy Swiss-Prot
Match: P30302 (Aquaporin PIP2-3 OS=Arabidopsis thaliana OX=3702 GN=PIP2-3 PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 4.6e-38
Identity = 84/119 (70.59%), Postives = 87/119 (73.11%), Query Frame = 0

Query: 1   MTAQSLGAICGCALVKAFQNAHYNKYGGRANSLADDYNIGTGLATEIIGTFVL------- 60
           M AQ LGAICG   VKAFQ++HY  YGG AN LAD YN GTGLA EIIGTFVL       
Sbjct: 127 MVAQCLGAICGVGFVKAFQSSHYVNYGGGANFLADGYNTGTGLAAEIIGTFVLVYTVFSA 186

Query: 61  -------------VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDD 100
                        VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAV+FNKSKPWDD
Sbjct: 187 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKSKPWDD 245

BLAST of Bhi08G000254 vs. ExPASy Swiss-Prot
Match: P43286 (Aquaporin PIP2-1 OS=Arabidopsis thaliana OX=3702 GN=PIP2-1 PE=1 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 3.9e-37
Identity = 81/117 (69.23%), Postives = 88/117 (75.21%), Query Frame = 0

Query: 3   AQSLGAICGCALVKAFQNAHYNKYGGRANSLADDYNIGTGLATEIIGTFVL--------- 62
           AQ LGAICG   VKAFQ+++Y +YGG ANSLAD Y+ GTGLA EIIGTFVL         
Sbjct: 131 AQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGTFVLVYTVFSATD 190

Query: 63  -----------VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDD 100
                      VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAV++NKSKPWDD
Sbjct: 191 PKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKSKPWDD 247

BLAST of Bhi08G000254 vs. ExPASy Swiss-Prot
Match: Q9FF53 (Probable aquaporin PIP2-4 OS=Arabidopsis thaliana OX=3702 GN=PIP2-4 PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 1.5e-36
Identity = 79/120 (65.83%), Postives = 86/120 (71.67%), Query Frame = 0

Query: 1   MTAQSLGAICGCALVKAFQNAHYNKYGGRANSLADDYNIGTGLATEIIGTFVL------- 60
           + AQ LGAICGC  VKAFQ+++Y +YGG AN LAD YN GTGL  EIIGTFVL       
Sbjct: 129 IVAQCLGAICGCGFVKAFQSSYYTRYGGGANELADGYNKGTGLGAEIIGTFVLVYTVFSA 188

Query: 61  -------------VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDDQ 101
                        VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAV++N  K WDDQ
Sbjct: 189 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNEKAWDDQ 248

BLAST of Bhi08G000254 vs. ExPASy Swiss-Prot
Match: Q84RL7 (Aquaporin PIP2-1 OS=Zea mays OX=4577 GN=PIP2-1 PE=1 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 4.7e-35
Identity = 77/119 (64.71%), Postives = 87/119 (73.11%), Query Frame = 0

Query: 1   MTAQSLGAICGCALVKAFQNAHYNKYGGRANSLADDYNIGTGLATEIIGTFVL------- 60
           + AQ LGAICG  LVKAFQ+A++++YGG ANSLA  Y+ GTGL  EIIGTFVL       
Sbjct: 134 IVAQCLGAICGVGLVKAFQSAYFDRYGGGANSLASGYSRGTGLGAEIIGTFVLVYTVFSA 193

Query: 61  -------------VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDD 100
                        VLAPLPIGFAVFMVHLATIP+TGTGINPARS GAAV++NK KPWDD
Sbjct: 194 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYNKDKPWDD 252

BLAST of Bhi08G000254 vs. ExPASy TrEMBL
Match: V5RDL5 (Plasma intrinsic protein 2-1 OS=Cucumis sativus OX=3659 GN=PIP2-1 PE=2 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.7e-40
Identity = 92/120 (76.67%), Postives = 94/120 (78.33%), Query Frame = 0

Query: 1   MTAQSLGAICGCALVKAFQNAHYNKYGGRANSLADDYNIGTGLATEIIGTFVL------- 60
           M AQSLGAICGCALVKAFQNAHY +YGG ANSLAD Y+ GTGLA EIIGTFVL       
Sbjct: 129 MAAQSLGAICGCALVKAFQNAHYTEYGGGANSLADGYSTGTGLAAEIIGTFVLVYTVFSA 188

Query: 61  -------------VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDDQ 101
                        VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDDQ
Sbjct: 189 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDDQ 248

BLAST of Bhi08G000254 vs. ExPASy TrEMBL
Match: A0A6J1IPG6 (aquaporin PIP2-1-like OS=Cucurbita maxima OX=3661 GN=LOC111478765 PE=3 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 5.1e-40
Identity = 91/120 (75.83%), Postives = 93/120 (77.50%), Query Frame = 0

Query: 1   MTAQSLGAICGCALVKAFQNAHYNKYGGRANSLADDYNIGTGLATEIIGTFVL------- 60
           M AQSLGAICGCALVKAFQN HYN+YGG ANSLAD Y+ GTGLA EIIGTFVL       
Sbjct: 129 MAAQSLGAICGCALVKAFQNGHYNEYGGGANSLADGYSTGTGLAAEIIGTFVLVYTVFSA 188

Query: 61  -------------VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDDQ 101
                        VLAPLPIG AVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDDQ
Sbjct: 189 TDPKRNARDSHVPVLAPLPIGLAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDDQ 248

BLAST of Bhi08G000254 vs. ExPASy TrEMBL
Match: A0A6J1ECG4 (aquaporin PIP2-1-like OS=Cucurbita moschata OX=3662 GN=LOC111432950 PE=3 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 5.1e-40
Identity = 92/120 (76.67%), Postives = 93/120 (77.50%), Query Frame = 0

Query: 1   MTAQSLGAICGCALVKAFQNAHYNKYGGRANSLADDYNIGTGLATEIIGTFVL------- 60
           M AQSLGAICGCALVKAFQN HY KYGG ANSLAD Y+ GTGLA EIIGTFVL       
Sbjct: 129 MAAQSLGAICGCALVKAFQNGHYIKYGGGANSLADGYSSGTGLAAEIIGTFVLVYTVFSA 188

Query: 61  -------------VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDDQ 101
                        VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDDQ
Sbjct: 189 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDDQ 248

BLAST of Bhi08G000254 vs. ExPASy TrEMBL
Match: A0A5A7TUM4 (Aquaporin PIP2-1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G00560 PE=3 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 3.3e-39
Identity = 89/120 (74.17%), Postives = 93/120 (77.50%), Query Frame = 0

Query: 1   MTAQSLGAICGCALVKAFQNAHYNKYGGRANSLADDYNIGTGLATEIIGTFVL------- 60
           + AQSLGAICGCALVKAFQN HY +YGG ANSLAD Y+ GTGLA EIIGTFVL       
Sbjct: 129 VAAQSLGAICGCALVKAFQNGHYTEYGGGANSLADGYSTGTGLAAEIIGTFVLVYTVFSA 188

Query: 61  -------------VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDDQ 101
                        VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFN+SKPWDDQ
Sbjct: 189 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNRSKPWDDQ 248

BLAST of Bhi08G000254 vs. ExPASy TrEMBL
Match: A0A1S3BMI9 (aquaporin PIP2-1-like OS=Cucumis melo OX=3656 GN=LOC103491188 PE=3 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 3.3e-39
Identity = 89/120 (74.17%), Postives = 93/120 (77.50%), Query Frame = 0

Query: 1   MTAQSLGAICGCALVKAFQNAHYNKYGGRANSLADDYNIGTGLATEIIGTFVL------- 60
           + AQSLGAICGCALVKAFQN HY +YGG ANSLAD Y+ GTGLA EIIGTFVL       
Sbjct: 129 VAAQSLGAICGCALVKAFQNGHYTEYGGGANSLADGYSTGTGLAAEIIGTFVLVYTVFSA 188

Query: 61  -------------VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKSKPWDDQ 101
                        VLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFN+SKPWDDQ
Sbjct: 189 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNRSKPWDDQ 248

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G37170.11.5e-3969.75plasma membrane intrinsic protein 2 [more]
AT2G37180.13.2e-3970.59Aquaporin-like superfamily protein [more]
AT3G53420.12.7e-3869.23plasma membrane intrinsic protein 2A [more]
AT3G53420.22.7e-3869.23plasma membrane intrinsic protein 2A [more]
AT5G60660.11.0e-3765.83plasma membrane intrinsic protein 2;4 [more]
Match NameE-valueIdentityDescription
P432872.1e-3869.75Aquaporin PIP2-2 OS=Arabidopsis thaliana OX=3702 GN=PIP2-2 PE=1 SV=2[more]
P303024.6e-3870.59Aquaporin PIP2-3 OS=Arabidopsis thaliana OX=3702 GN=PIP2-3 PE=1 SV=1[more]
P432863.9e-3769.23Aquaporin PIP2-1 OS=Arabidopsis thaliana OX=3702 GN=PIP2-1 PE=1 SV=1[more]
Q9FF531.5e-3665.83Probable aquaporin PIP2-4 OS=Arabidopsis thaliana OX=3702 GN=PIP2-4 PE=1 SV=1[more]
Q84RL74.7e-3564.71Aquaporin PIP2-1 OS=Zea mays OX=4577 GN=PIP2-1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
V5RDL51.7e-4076.67Plasma intrinsic protein 2-1 OS=Cucumis sativus OX=3659 GN=PIP2-1 PE=2 SV=1[more]
A0A6J1IPG65.1e-4075.83aquaporin PIP2-1-like OS=Cucurbita maxima OX=3661 GN=LOC111478765 PE=3 SV=1[more]
A0A6J1ECG45.1e-4076.67aquaporin PIP2-1-like OS=Cucurbita moschata OX=3662 GN=LOC111432950 PE=3 SV=1[more]
A0A5A7TUM43.3e-3974.17Aquaporin PIP2-1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold90... [more]
A0A1S3BMI93.3e-3974.17aquaporin PIP2-1-like OS=Cucumis melo OX=3656 GN=LOC103491188 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 55..98
e-value: 6.3E-13
score: 49.0
coord: 1..55
e-value: 4.1E-10
score: 39.7
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 1..101
e-value: 8.3E-26
score: 92.6
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 2..94
IPR034294Aquaporin transporterPANTHERPTHR45687AQUAPORIN OR AQUAGLYCEROPORIN RELATEDcoord: 51..100
coord: 1..55
NoneNo IPR availablePANTHERPTHR45687:SF75BNAC03G72010D PROTEINcoord: 51..100
coord: 1..55

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M000254Bhi08M000254mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015250 water channel activity
molecular_function GO:0015267 channel activity