Bhi08G000009 (gene) Wax gourd (B227) v1

Overview


Name	Bhi08G000009
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	rRNA intron-encoded homing endonuclease, putative
Location	chr8: 254479 .. 255161 (-)
RNA-Seq Expression	Bhi08G000009
Synteny	Bhi08G000009

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGAAGAGAACCATGGTGTCACCCGGTCTAATAATGACTGTAAAAAGCTCGTAGTTGGACCTTGGGTTGGGTCGATCGGTCTGCCTATGGTGGGCACCCGTCGGCTTGTCCCTTCTGGCAGCGATGAGCTCCTAGCTTTAACTGGCCAGGTCGTGCCTCCGACGTTATTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTCTGTTGGCCTTCGAGATCGGAGTAATGATTAACAGGGACAGTCGAGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTGAGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATTGGCGGATGTTGCTTTAAGGACTCCGCCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCCCCAGGAAGGGAGCTTGTGGCTTACTTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGATAGACTAAGAGCTCTTTCTTGA

mRNA sequence

ATGGTTGAAGAGAACCATGGTGTCACCCGGTCTAATAATGACTGTAAAAAGCTCGTAGTTGGACCTTGGGTTGGGTCGATCGGTCTGCCTATGGTGGGCACCCGTCGGCTTGTCCCTTCTGGCAGCGATGAGCTCCTAGCTTTAACTGGCCAGGATTTCGATCCTATTCTGTTGGCCTTCGAGATCGGAGTAATGATTAACAGGGACAGTCGAGGGCATTCACGAACAACTGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTGAGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATTGGCGGATGTTGCTTTAAGGACTCCGCCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCCCCAGGAAGGGAGCTTGTGGCTTACTTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGATAGACTAAGAGCTCTTTCTTGA

Coding sequence (CDS)

ATGGTTGAAGAGAACCATGGTGTCACCCGGTCTAATAATGACTGTAAAAAGCTCGTAGTTGGACCTTGGGTTGGGTCGATCGGTCTGCCTATGGTGGGCACCCGTCGGCTTGTCCCTTCTGGCAGCGATGAGCTCCTAGCTTTAACTGGCCAGGATTTCGATCCTATTCTGTTGGCCTTCGAGATCGGAGTAATGATTAACAGGGACAGTCGAGGGCATTCACGAACAACTGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTGAGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATTGGCGGATGTTGCTTTAAGGACTCCGCCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCCCCAGGAAGGGAGCTTGTGGCTTACTTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGATAGACTAAGAGCTCTTTCTTGA

Protein sequence

MVEENHGVTRSNNDCKKLVVGPWVGSIGLPMVGTRRLVPSGSDELLALTGQDFDPILLAFEIGVMINRDSRGHSRTTAKAFAKDVFINQERKLRARRRSDTVLVSTINDADQGLADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGRELVAYFDSTRGNLPGPDIVRIDRLRALS

Homology

[Close]

BLAST of Bhi08G000009 vs. ExPASy TrEMBL
Match: A0A072TTZ2 (rRNA intron-encoded homing endonuclease, putative OS=Medicago truncatula OX=3880 GN=MTR_0052s0110 PE=4 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 5.2e-59
Identity = 125/144 (86.81%), Postives = 127/144 (88.19%), Query Frame = 0


Query: 16  KKLVVGPWVGSIGLPMVGTRRLVPSGSDELLALTGQDFDPILLAFEIGVMINRDSRGHSR 75
           KKLVVGPWVGSIG PMV T  LVPS  D LLAL G+D DPI+LAF IGVMINRDSRGHSR
Sbjct: 197 KKLVVGPWVGSIGPPMVCTGWLVPSAGDALLALIGRDSDPIVLAFGIGVMINRDSRGHSR 256

Query: 76  TTAKAFAKDVFINQERKLRARRRSDTVLVSTINDADQGLADVALRTPPAPYEKSKSLGSG 135
           TTAKAFAKDVFINQERKL ARRRSDTVLVSTINDADQG ADVA RTP APYEKSKSLGSG
Sbjct: 257 TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSG 316

Query: 136 GSMVARLKLKGIDGRAPPGRELVA 160
           GSMVARLKLKGIDGRAPPG E  A
Sbjct: 317 GSMVARLKLKGIDGRAPPGVEPAA 340

[Close]

BLAST of Bhi08G000009 vs. ExPASy TrEMBL
Match: A0A4D6NNZ3 (Uncharacterized protein OS=Vigna unguiculata OX=3917 GN=DEO72_LG11g1248 PE=4 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 7.5e-50
Identity = 115/153 (75.16%), Postives = 123/153 (80.39%), Query Frame = 0


Query: 16  KKLVVGPWVGSIGLPMVGTRRLVPSGSDELLALTGQ----DFDPILLAFEIGVMINRDSR 75
           KKLVVGPWVGSIG P++ T  LVPS  D LLALTG+    D DP++LAF IGVMINRDSR
Sbjct: 182 KKLVVGPWVGSIGPPLMCTGLLVPSADDALLALTGRVVPPDSDPLVLAFGIGVMINRDSR 241

Query: 76  GHS-----RTTAKAFAKDVFINQERKLRARRRSDTVLVSTINDADQGLADVALRTPPAPY 135
           G+S     RTT KAFAKDVFINQERKL ARRRSDTVLVSTINDADQG A+VA RTP APY
Sbjct: 242 GNSWIYERRTTVKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSANVAFRTPLAPY 301

Query: 136 EKSKSLGSGGSMVARLKLKGIDGRAPPGRELVA 160
           EKSKSLGS GSMV  +KLKGIDGRAP G E  A
Sbjct: 302 EKSKSLGSRGSMVVGMKLKGIDGRAPLGVEPAA 334

[Close]

BLAST of Bhi08G000009 vs. ExPASy TrEMBL
Match: A0A4U5QPX7 (Uncharacterized protein OS=Populus alba OX=43335 GN=D5086_0000063000 PE=4 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 1.4e-48
Identity = 117/162 (72.22%), Postives = 121/162 (74.69%), Query Frame = 0


Query: 2   VEENHGVTRSNNDCKKLVVGPWVGSIGLPMVGTRRLVPSGSDELLALTGQ----DFDPIL 61
           +E  HGV       KKLVVG WVGS G P V T RLVPS  D LLALTG+    DFDPI+
Sbjct: 28  IEAYHGV-------KKLVVGLWVGSAGPPQVCTGRLVPSTGDALLALTGRVVPPDFDPIV 87

Query: 62  LAFEIGVMINRDSRGHSRTTAKAFAKDVFINQERKLRARRRSDTVLVSTINDADQGLADV 121
           LAF IGVMINRDSRGHS           FI +ERKL ARRRSDTVLVSTINDADQGLADV
Sbjct: 88  LAFGIGVMINRDSRGHS----------YFIVRERKLGARRRSDTVLVSTINDADQGLADV 147

Query: 122 ALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGRELVA 160
           A RTPPAPYEKSK LGSGGSMVARLKLKGIDGRAPPG E  A
Sbjct: 148 ASRTPPAPYEKSKFLGSGGSMVARLKLKGIDGRAPPGVEPAA 172

[Close]

BLAST of Bhi08G000009 vs. ExPASy TrEMBL
Match: A0A4U5Q6S3 (Uncharacterized protein OS=Populus alba OX=43335 GN=D5086_0000127820 PE=4 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 1.4e-48
Identity = 118/164 (71.95%), Postives = 120/164 (73.17%), Query Frame = 0


Query: 16  KKLVVGPWVGSIGLPMVGTRRLVPSGSDELLAL------TGQDFDPILLAFEIGVMINRD 75
           KKLVVG WVGS G P V T RLVPS  D LLAL      +G DFDPI+LAF IGVMINRD
Sbjct: 165 KKLVVGLWVGSAGPPQVCTGRLVPSTGDALLALNWPGRASGADFDPIVLAFGIGVMINRD 224

Query: 76  SRGHS----RTTAKAFAKD----------VFINQERKLRARRRSDTVLVSTINDADQGLA 135
           SRGHS    R     F KD           FINQERKL ARRRSDTVLVSTINDADQGLA
Sbjct: 225 SRGHSYFIVRGEILGFMKDEQLRKHLPRMFFINQERKLGARRRSDTVLVSTINDADQGLA 284

Query: 136 DVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGRELVA 160
           DVA RTPPAPYEKSK LGSGGSMVARLKLKGIDGRAPPG E  A
Sbjct: 285 DVASRTPPAPYEKSKFLGSGGSMVARLKLKGIDGRAPPGVEPAA 328

[Close]

BLAST of Bhi08G000009 vs. ExPASy TrEMBL
Match: A0A4U5PVW4 (Uncharacterized protein OS=Populus alba OX=43335 GN=D5086_0000127940 PE=4 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 5.4e-48
Identity = 113/148 (76.35%), Postives = 116/148 (78.38%), Query Frame = 0


Query: 16  KKLVVGPWVGSIGLPMVGTRRLVPSGSDELLALTGQ----DFDPILLAFEIGVMINRDSR 75
           KKLVVG WVGS G P V T RLVPS  D LLALTG+    DFDPI+LAF IGVMINRDSR
Sbjct: 506 KKLVVGLWVGSAGPPQVCTGRLVPSTGDALLALTGRVVPPDFDPIVLAFGIGVMINRDSR 565

Query: 76  GHSRTTAKAFAKDVFINQERKLRARRRSDTVLVSTINDADQGLADVALRTPPAPYEKSKS 135
           GHS           FI +ERKL ARRRSDTVLVSTINDADQGLADVA RTPPAPYEKSK 
Sbjct: 566 GHS----------YFIVRERKLGARRRSDTVLVSTINDADQGLADVASRTPPAPYEKSKF 625

Query: 136 LGSGGSMVARLKLKGIDGRAPPGRELVA 160
           LGSGGSMVARLKLKGIDGRAPPG E  A
Sbjct: 626 LGSGGSMVARLKLKGIDGRAPPGVEPAA 643

The following BLAST results are available for this feature:

BLAST of Bhi08G000009 vs.


Match Name	E-value	Identity	Description

BLAST of Bhi08G000009 vs.


Match Name	E-value	Identity	Description

BLAST of Bhi08G000009 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A072TTZ2	5.2e-59	86.81	rRNA intron-encoded homing endonuclease, putative OS=Medicago truncatula OX=3880...	[more]
A0A4D6NNZ3	7.5e-50	75.16	Uncharacterized protein OS=Vigna unguiculata OX=3917 GN=DEO72_LG11g1248 PE=4 SV=...	[more]
A0A4U5QPX7	1.4e-48	72.22	Uncharacterized protein OS=Populus alba OX=43335 GN=D5086_0000063000 PE=4 SV=1	[more]
A0A4U5Q6S3	1.4e-48	71.95	Uncharacterized protein OS=Populus alba OX=43335 GN=D5086_0000127820 PE=4 SV=1	[more]
A0A4U5PVW4	5.4e-48	76.35	Uncharacterized protein OS=Populus alba OX=43335 GN=D5086_0000127940 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34410	INTRON-ENCODED HOMING ENDONUCLEASE, PUTATIVE-RELATED	coord: 16..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi08M000009	Bhi08M000009	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0004519	endonuclease activity