Bhi07G001465 (gene) Wax gourd (B227) v1

Overview


Name	Bhi07G001465
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Pectin lyase-like superfamily protein
Location	chr7: 49615729 .. 49616603 (-)
RNA-Seq Expression	Bhi07G001465
Synteny	Bhi07G001465

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGGAATTCACATCTCGAAGTCAACTAACATTGACATCTTCAACTCAGTGATGAACGGAGATGACTACATCTCCATGGCTCTTGGAAGTAAAAACATCAATATAACCAATGTTCAATGTGGTCTAGGCCACGGAATCAACATCGGAAGCCTTGGCCAATATCCAAAAGAATTCAACGTGACCAAAATCACAGTAAGAGACTCCATGTTCAAAGACACCAAGAACGGTGTAAGGAATAAATCATGGTCGAATTCACATTCAAGTATGGTTAGCAAAGTCTCATTGATCCATATTCAAATGGAGAATGTAGAAAATCCTAGGAAAATTCTTATAAATATAAAAAATTAAAAAATATTTACACTTTATAGTAAAAAGTTCTAAAAATGTTTGTGCATTTGAAATTTTTTGTTATAAAATATAAATATTTTTAAATATTTTTTTATATTTAAAAAGACCCCTATCATAATTGATTACAACTATTGCCCCATTGCAATAGCAATCATCATTATCGTCGATCGGTATAATTTATATAAATTAATTTGAGAGATTATATACATATGATATTATTATTGTTCTAAAGCAAAATGTGTTTGGTTAATTACAAAGTAAATCAAGAGTGCAAATTCAAGATGTTAGATATGAAGACAATAGAGGGAGTTGAAGTAGCAAAGAGAGAGTAAAGTTTGATTGTAGCTCAATTAATCCATGTCAAGGCATAGTGTTGCAAGACATTAACTTGACATTCAACAATGGTGGTTCACCAATTTCAACCTGTTATAATAGGACAGGATCTGCTTTTGGTGTTCAATTACCTCCTCCTTGCTTATTACCAGAGACAGGGAAGAGATCAGTGGGAGGAGAGAGTTTTTTAA

mRNA sequence

ATGGATGGAATTCACATCTCGAAGTCAACTAACATTGACATCTTCAACTCAGTGATGAACGGAGATGACTACATCTCCATGGCTCTTGGAAGTAAAAACATCAATATAACCAATGTTCAATGTGGTCTAGGCCACGGAATCAACATCGGAAGCCTTGGCCAATATCCAAAAGAATTCAACGTGACCAAAATCACAGTAAGAGACTCCATGTTCAAAGACACCAAGAACGGTGTAAGGAATAAATCATGGTCGAATTCACATTCAAGTATGGATCTGCTTTTGGTGTTCAATTACCTCCTCCTTGCTTATTACCAGAGACAGGGAAGAGATCAGTGGGAGGAGAGAGTTTTTTAA

Coding sequence (CDS)

ATGGATGGAATTCACATCTCGAAGTCAACTAACATTGACATCTTCAACTCAGTGATGAACGGAGATGACTACATCTCCATGGCTCTTGGAAGTAAAAACATCAATATAACCAATGTTCAATGTGGTCTAGGCCACGGAATCAACATCGGAAGCCTTGGCCAATATCCAAAAGAATTCAACGTGACCAAAATCACAGTAAGAGACTCCATGTTCAAAGACACCAAGAACGGTGTAAGGAATAAATCATGGTCGAATTCACATTCAAGTATGGATCTGCTTTTGGTGTTCAATTACCTCCTCCTTGCTTATTACCAGAGACAGGGAAGAGATCAGTGGGAGGAGAGAGTTTTTTAA

Protein sequence

MDGIHISKSTNIDIFNSVMNGDDYISMALGSKNINITNVQCGLGHGINIGSLGQYPKEFNVTKITVRDSMFKDTKNGVRNKSWSNSHSSMDLLLVFNYLLLAYYQRQGRDQWEERVF

Homology

[Close]

BLAST of Bhi07G001465 vs. TAIR 10
Match: AT5G39910.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 90.1 bits (222), Expect = 1.3e-18
Identity = 43/83 (51.81%), Postives = 59/83 (71.08%), Query Frame = 0


Query: 2   DGIHISKSTNIDIFNS-VMNGDDYISMALGSKNINITNVQCGLGHGINIGSLGQYPKEFN 61
           DGIHI +S N+ +++S +  GDD IS+  GSK INI+ + CG GHGI+IGSLG+Y  +  
Sbjct: 197 DGIHIQRSQNVIVYDSNIRTGDDCISIGDGSKYINISRISCGPGHGISIGSLGRYGTKET 256

Query: 62  VTKITVRDSMFKDTKNGVRNKSW 84
           V  + VRD  F++T NGVR K+W
Sbjct: 257 VENVVVRDCTFRETTNGVRIKTW 279

[Close]

BLAST of Bhi07G001465 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 88.6 bits (218), Expect = 3.7e-18
Identity = 41/86 (47.67%), Postives = 60/86 (69.77%), Query Frame = 0


Query: 2   DGIHISKSTNIDIFNS-VMNGDDYISMALGSKNINITNVQCGLGHGINIGSLGQYPKEFN 61
           DGIHI +S  +++  + +  GDD +S+  G++N+ + NV+CG GHGI+IGSLG+YP E  
Sbjct: 241 DGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQP 300

Query: 62  VTKITVRDSMFKDTKNGVRNKSWSNS 87
           V  +TVR  + K+T NGVR K+W  S
Sbjct: 301 VKGVTVRKCLIKNTDNGVRIKTWPGS 326

[Close]

BLAST of Bhi07G001465 vs. TAIR 10
Match: AT3G07850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 88.6 bits (218), Expect = 3.7e-18
Identity = 41/86 (47.67%), Postives = 60/86 (69.77%), Query Frame = 0


Query: 2   DGIHISKSTNIDIFNS-VMNGDDYISMALGSKNINITNVQCGLGHGINIGSLGQYPKEFN 61
           DGIHI +S  +++  + +  GDD +S+  G++N+ + NV+CG GHGI+IGSLG+YP E  
Sbjct: 240 DGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQP 299

Query: 62  VTKITVRDSMFKDTKNGVRNKSWSNS 87
           V  +TVR  + K+T NGVR K+W  S
Sbjct: 300 VKGVTVRKCLIKNTDNGVRIKTWPGS 325

[Close]

BLAST of Bhi07G001465 vs. TAIR 10
Match: AT4G18180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 88.6 bits (218), Expect = 3.7e-18
Identity = 40/86 (46.51%), Postives = 59/86 (68.60%), Query Frame = 0


Query: 2   DGIHISKSTNIDIFNS-VMNGDDYISMALGSKNINITNVQCGLGHGINIGSLGQYPKEFN 61
           DGIHI +S+N+    S +  GDD +S+  G+  I IT+++CG GHGI++GSLG+YP E +
Sbjct: 205 DGIHIERSSNVYFSRSHIATGDDCVSIGQGNSQITITSIKCGPGHGISVGSLGRYPNEKD 264

Query: 62  VTKITVRDSMFKDTKNGVRNKSWSNS 87
           V  + V+D     T NG+R K+W+NS
Sbjct: 265 VNGLVVKDCKISGTTNGIRIKTWANS 290

[Close]

BLAST of Bhi07G001465 vs. TAIR 10
Match: AT1G17150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 87.0 bits (214), Expect = 1.1e-17
Identity = 47/100 (47.00%), Postives = 69/100 (69.00%), Query Frame = 0


Query: 2   DGIHISKSTNIDIFNS-VMNGDDYISMALGSKNINITNVQCGLGHGINIGSLGQYPKEFN 61
           DGI I+ S+N+ I N+ +  GDD I+M  G+ N +I NV+CG GHGI+IGSLG+   E N
Sbjct: 203 DGIKIALSSNMQISNTHISTGDDCIAMLSGNTNFDIYNVKCGPGHGISIGSLGKNKDEKN 262

Query: 62  VTKITVRDSMFKDTKNGVRNKSWSNSHSSMDLL-LVFNYL 100
           V  + VR+S+F  T NG+R K+W +S S++ ++ LV+  L
Sbjct: 263 VNGLMVRNSVFTGTTNGIRIKTWESSASTIRIINLVYENL 302

[Close]

BLAST of Bhi07G001465 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.4e-17
Identity = 44/86 (51.16%), Postives = 60/86 (69.77%), Query Frame = 0


Query: 2   DGIHISKSTNIDIFNS-VMNGDDYISMALGSKNINITNVQCGLGHGINIGSLGQYPKEFN 61
           DGIHI +S  ++I N+ +  GDD IS+  GSKNINITN+ CG GHGI++GSLG+Y  E +
Sbjct: 158 DGIHIGRSDGVNIINTEIKTGDDCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEES 217

Query: 62  VTKITVRDSMFKDTKNGVRNKSWSNS 87
           V  I V++     ++NGVR K+W  S
Sbjct: 218 VVGIYVKNCTITGSQNGVRIKTWPKS 243

[Close]

BLAST of Bhi07G001465 vs. ExPASy Swiss-Prot
Match: P49063 (Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 PE=2 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 5.1e-17
Identity = 41/86 (47.67%), Postives = 60/86 (69.77%), Query Frame = 0


Query: 2   DGIHISKSTNIDIFNS-VMNGDDYISMALGSKNINITNVQCGLGHGINIGSLGQYPKEFN 61
           DGIHI +S  +++  + +  GDD +S+  G++N+ + NV+CG GHGI+IGSLG+YP E  
Sbjct: 240 DGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQP 299

Query: 62  VTKITVRDSMFKDTKNGVRNKSWSNS 87
           V  +TVR  + K+T NGVR K+W  S
Sbjct: 300 VKGVTVRKCLIKNTDNGVRIKTWPGS 325

[Close]

BLAST of Bhi07G001465 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 8.8e-17
Identity = 48/101 (47.52%), Postives = 60/101 (59.41%), Query Frame = 0


Query: 2   DGIHISKSTNIDIFNS-VMNGDDYISMALGSKNINITNVQCGLGHGINIGSLGQYPKEFN 61
           DGIH+  S  + I N+ +  GDD IS+  GS+N+ IT V CG GHGI+IGSLG+Y  E  
Sbjct: 179 DGIHVGISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKE 238

Query: 62  VTKITVRDSMFKDTKNGVRNKSWSNSHSSMDLLLVFNYLLL 102
           V  ITV+   F  T NGVR K+W NS       L F  L +
Sbjct: 239 VRGITVKGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTM 279

[Close]

BLAST of Bhi07G001465 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.5e-16
Identity = 46/118 (38.98%), Postives = 72/118 (61.02%), Query Frame = 0


Query: 2   DGIHISKSTNIDIFNSVM-NGDDYISMALGSKNINITNVQCGLGHGINIGSLGQYPKEFN 61
           DGIH+  + ++ I+NSV+ NGDD IS+  G  +++I  V CG  HGI+IGSLG +  +  
Sbjct: 261 DGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVHNSQAC 320

Query: 62  VTKITVRDSMFKDTKNGVRNKSWSNSHSSMDLLL--------VFNYLLLAYYQRQGRD 111
           V+ ITVR+++ +D+ NG+R K+W     S+  LL        V N +++  Y  Q +D
Sbjct: 321 VSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQSKD 378

[Close]

BLAST of Bhi07G001465 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 4.4e-16
Identity = 44/88 (50.00%), Postives = 60/88 (68.18%), Query Frame = 0


Query: 2   DGIHISKSTNIDIFNSVMN-GDDYISMALGSKNINITNVQCGLGHGINIGSLGQYPKEFN 61
           DGIH+  S+ + I N+V+  GDD IS+  G+  +NIT V CG GHGI+IGSLG+Y  E +
Sbjct: 212 DGIHMGDSSGVTITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKD 271

Query: 62  VTKITVRDSMFKDTKNGVRNKSWSNSHS 89
           VT I V+D   K T NGVR K++ ++ S
Sbjct: 272 VTDINVKDCTLKKTANGVRIKAYEDAAS 299

[Close]

BLAST of Bhi07G001465 vs. ExPASy TrEMBL
Match: A0A6J1DTV4 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111024296 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 3.2e-22
Identity = 61/90 (67.78%), Postives = 70/90 (77.78%), Query Frame = 0


Query: 2   DGIHISKSTNIDIFNSVM-NGDDYISMALGSKNINITNVQCGLGHGINIGSLGQYPKEFN 61
           DGIHIS+ST I+I NSV+  GDD IS+  GSK+INITNV CG GHGI+IGSLG+YP E +
Sbjct: 156 DGIHISRSTGINIKNSVIGTGDDCISLGPGSKDINITNVHCGPGHGISIGSLGRYPNEED 215

Query: 62  VTKITVRDSMFKDTKNGVRNKSWSNSHSSM 91
           VT ITVRDS F  T NGVR KSWS S  S+
Sbjct: 216 VTGITVRDSTFTGTTNGVRIKSWSTSLPSV 245

[Close]

BLAST of Bhi07G001465 vs. ExPASy TrEMBL
Match: A0A6J1L3F3 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111500172 PE=3 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 5.5e-22
Identity = 58/96 (60.42%), Postives = 71/96 (73.96%), Query Frame = 0


Query: 2   DGIHISKSTNIDIFNSVM-NGDDYISMALGSKNINITNVQCGLGHGINIGSLGQYPKEFN 61
           DGIHISKST + I NS++  GDD IS   GSK++NITNV CG GHGI+IGSLG+Y  E N
Sbjct: 150 DGIHISKSTGVTIMNSIIATGDDCISFGPGSKDVNITNVHCGPGHGISIGSLGKYQNEPN 209

Query: 62  VTKITVRDSMFKDTKNGVRNKSWSNSHSSMDLLLVF 97
           +  ITVRDS F  TKNG++ K+WS S+SS+   L F
Sbjct: 210 IDGITVRDSKFVGTKNGIQIKTWSKSYSSVVSNLTF 245

[Close]

BLAST of Bhi07G001465 vs. ExPASy TrEMBL
Match: A0A5D3BB58 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001870 PE=3 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 7.2e-22
Identity = 58/90 (64.44%), Postives = 70/90 (77.78%), Query Frame = 0


Query: 2   DGIHISKSTNIDIFNSVM-NGDDYISMALGSKNINITNVQCGLGHGINIGSLGQYPKEFN 61
           DGIHIS S+ I+I +S +  GDD IS+  GSK+INITNV CG GHGI+IGSLG+YP E N
Sbjct: 154 DGIHISSSSGINIMHSTIGTGDDCISLGPGSKSINITNVYCGPGHGISIGSLGKYPNEEN 213

Query: 62  VTKITVRDSMFKDTKNGVRNKSWSNSHSSM 91
           V ++TVRDS F  T NG R KSWS+S+SSM
Sbjct: 214 VFEVTVRDSTFVGTTNGARIKSWSSSYSSM 243

[Close]

BLAST of Bhi07G001465 vs. ExPASy TrEMBL
Match: A0A0A0L7H9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G063610 PE=3 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.6e-21
Identity = 57/90 (63.33%), Postives = 71/90 (78.89%), Query Frame = 0


Query: 2   DGIHISKSTNIDIFNSVM-NGDDYISMALGSKNINITNVQCGLGHGINIGSLGQYPKEFN 61
           DGIHIS S+ I+I +S +  GDD IS+  GSK INITN+QCG GHGI+IGSLG+YP E +
Sbjct: 162 DGIHISSSSGINIMHSTIGTGDDCISLGPGSKFINITNIQCGPGHGISIGSLGKYPNEED 221

Query: 62  VTKITVRDSMFKDTKNGVRNKSWSNSHSSM 91
           V ++TVRDS F  T NGVR K+WS+S+SSM
Sbjct: 222 VFEVTVRDSTFIGTSNGVRIKTWSSSYSSM 251

[Close]

BLAST of Bhi07G001465 vs. ExPASy TrEMBL
Match: A0A6J1F153 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111438612 PE=3 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 2.1e-21
Identity = 56/96 (58.33%), Postives = 74/96 (77.08%), Query Frame = 0


Query: 2   DGIHISKSTNIDIFNSVM-NGDDYISMALGSKNINITNVQCGLGHGINIGSLGQYPKEFN 61
           DGIH+SKS+ I+I +SV+  GDD IS+  GSK INIT++QCG GHGI+IGSLG+YP E +
Sbjct: 186 DGIHVSKSSGINIMHSVIRTGDDCISLGPGSKFINITDIQCGPGHGISIGSLGKYPNEED 245

Query: 62  VTKITVRDSMFKDTKNGVRNKSWSNSHSSMDLLLVF 97
           V+++T+RDS F  T NGVR K+WS+S SS    + F
Sbjct: 246 VSEVTIRDSTFVGTDNGVRIKTWSSSFSSFVSKIAF 281

The following BLAST results are available for this feature:

BLAST of Bhi07G001465 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G39910.1	1.3e-18	51.81	Pectin lyase-like superfamily protein	[more]
AT3G14040.1	3.7e-18	47.67	Pectin lyase-like superfamily protein	[more]
AT3G07850.1	3.7e-18	47.67	Pectin lyase-like superfamily protein	[more]
AT4G18180.1	3.7e-18	46.51	Pectin lyase-like superfamily protein	[more]
AT1G17150.1	1.1e-17	47.00	Pectin lyase-like superfamily protein	[more]

BLAST of Bhi07G001465 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24548	1.4e-17	51.16	Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1	[more]
P49063	5.1e-17	47.67	Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 ...	[more]
Q6H9K0	8.8e-17	47.52	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]
Q949Z1	1.5e-16	38.98	Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV...	[more]
P35339	4.4e-16	50.00	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1	[more]

BLAST of Bhi07G001465 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DTV4	3.2e-22	67.78	exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111024296 PE=3 SV...	[more]
A0A6J1L3F3	5.5e-22	60.42	exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111500172 PE=3 SV=1	[more]
A0A5D3BB58	7.2e-22	64.44	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A0A0L7H9	1.6e-21	63.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G063610 PE=3 SV=1	[more]
A0A6J1F153	2.1e-21	58.33	exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111438612 PE=3 SV=...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 2..87 e-value: 9.5E-25 score: 87.3
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 38..51
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 1..97 e-value: 5.0E-24 score: 86.7
None	No IPR available	PANTHER	PTHR31375:SF124	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN	coord: 1..88
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 1..88
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 2..87

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi07M001465	Bhi07M001465	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity