Bhi07G001454 (gene) Wax gourd (B227) v1

Overview


Name	Bhi07G001454
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	cytochrome P450 71B37-like
Location	chr7: 49282741 .. 49282995 (-)
RNA-Seq Expression	Bhi07G001454
Synteny	Bhi07G001454

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAACGATGTAGATTACAGAGGACAAAATTTTGAGTTATTACCATTTGGAGCTGGTCGGAGAAGTTGTCCAGGGATGAACATGGCGGTCGTTGCGGTGGAGTTAACATTGGCTAATCTATTGCTTCGTTTTGATTGGAAATTACCAGATGGAATGAAAGAAGAAGATGTAGACATGGAAGAGGATGTTGGTCTGACTGTTACTAAGAAATCACCTCTTAAACTTGTTCCACTCAACTTCTCAAGAGTTTAG

mRNA sequence

ATGGGAAACGATGTAGATTACAGAGGACAAAATTTTGAGTTATTACCATTTGGAGCTGGTCGGAGAAGTTGTCCAGGGATGAACATGGCGGTCGTTGCGGTGGAGTTAACATTGGCTAATCTATTGCTTCGTTTTGATTGGAAATTACCAGATGGAATGAAAGAAGAAGATGTAGACATGGAAGAGGATGTTGGTCTGACTGTTACTAAGAAATCACCTCTTAAACTTGTTCCACTCAACTTCTCAAGAGTTTAG

Coding sequence (CDS)

ATGGGAAACGATGTAGATTACAGAGGACAAAATTTTGAGTTATTACCATTTGGAGCTGGTCGGAGAAGTTGTCCAGGGATGAACATGGCGGTCGTTGCGGTGGAGTTAACATTGGCTAATCTATTGCTTCGTTTTGATTGGAAATTACCAGATGGAATGAAAGAAGAAGATGTAGACATGGAAGAGGATGTTGGTCTGACTGTTACTAAGAAATCACCTCTTAAACTTGTTCCACTCAACTTCTCAAGAGTTTAG

Protein sequence

MGNDVDYRGQNFELLPFGAGRRSCPGMNMAVVAVELTLANLLLRFDWKLPDGMKEEDVDMEEDVGLTVTKKSPLKLVPLNFSRV

Homology

[Close]

BLAST of Bhi07G001454 vs. TAIR 10
Match: AT5G25140.1 (cytochrome P450, family 71, subfamily B, polypeptide 13 )

HSP 1 Score: 113.6 bits (283), Expect = 7.6e-26
Identity = 49/79 (62.03%), Postives = 61/79 (77.22%), Query Frame = 0


Query: 1   MGNDVDYRGQNFELLPFGAGRRSCPGMNMAVVAVELTLANLLLRFDWKLPDGMKEEDVDM 60
           M N +DY+G NFELLPFG+GRR CPG+ M +  + LTL NLL RFDWKLP+GM+ EDVD+
Sbjct: 413 MDNQIDYKGLNFELLPFGSGRRICPGIGMGMALIHLTLINLLYRFDWKLPEGMEVEDVDL 472

Query: 61  EEDVGLTVTKKSPLKLVPL 80
           EE  GL   KK PL+L+P+
Sbjct: 473 EESYGLVCPKKVPLELIPV 491

[Close]

BLAST of Bhi07G001454 vs. TAIR 10
Match: AT5G25120.1 (ytochrome p450, family 71, subfamily B, polypeptide 11 )

HSP 1 Score: 112.8 bits (281), Expect = 1.3e-25
Identity = 49/79 (62.03%), Postives = 60/79 (75.95%), Query Frame = 0


Query: 1   MGNDVDYRGQNFELLPFGAGRRSCPGMNMAVVAVELTLANLLLRFDWKLPDGMKEEDVDM 60
           M N +DY+G NFE LPFG+GRR CPG+ M +  V LTL NLL RFDWKLP+GM+ EDVD+
Sbjct: 413 MDNQIDYKGLNFEFLPFGSGRRMCPGIGMGMALVHLTLINLLYRFDWKLPEGMEVEDVDL 472

Query: 61  EEDVGLTVTKKSPLKLVPL 80
           EE  GL   KK PL+L+P+
Sbjct: 473 EESYGLVCPKKVPLQLIPV 491

[Close]

BLAST of Bhi07G001454 vs. TAIR 10
Match: AT3G26170.1 (cytochrome P450, family 71, subfamily B, polypeptide 19 )

HSP 1 Score: 111.3 bits (277), Expect = 3.8e-25
Identity = 52/79 (65.82%), Postives = 61/79 (77.22%), Query Frame = 0


Query: 1   MGNDVDYRGQNFELLPFGAGRRSCPGMNMAVVAVELTLANLLLRFDWKLPDGMKEEDVDM 60
           M + VDYRGQ++ELLPFG+GRR CPGM M + AVEL L NLL  FDWKLPDGM  +D+D 
Sbjct: 421 MDSFVDYRGQHYELLPFGSGRRICPGMPMGIAAVELGLLNLLYFFDWKLPDGMTHKDIDT 480

Query: 61  EEDVGLTVTKKSPLKLVPL 80
           EE   LT+ KK PLKLVP+
Sbjct: 481 EEAGTLTIVKKVPLKLVPV 499

[Close]

BLAST of Bhi07G001454 vs. TAIR 10
Match: AT5G25130.1 (cytochrome P450, family 71, subfamily B, polypeptide 12 )

HSP 1 Score: 111.3 bits (277), Expect = 3.8e-25
Identity = 49/79 (62.03%), Postives = 60/79 (75.95%), Query Frame = 0


Query: 1   MGNDVDYRGQNFELLPFGAGRRSCPGMNMAVVAVELTLANLLLRFDWKLPDGMKEEDVDM 60
           M + +DY+G NFELLPFG+GRR CPG+ M +  V LTL NLL RFDWKLP+GMK  DVD+
Sbjct: 413 MDSQIDYKGLNFELLPFGSGRRICPGIGMGMALVHLTLINLLYRFDWKLPEGMKVADVDL 472

Query: 61  EEDVGLTVTKKSPLKLVPL 80
           EE  GL   KK PL+L+P+
Sbjct: 473 EESYGLVCPKKIPLQLIPV 491

[Close]

BLAST of Bhi07G001454 vs. TAIR 10
Match: AT3G26180.1 (cytochrome P450, family 71, subfamily B, polypeptide 20 )

HSP 1 Score: 110.9 bits (276), Expect = 4.9e-25
Identity = 51/79 (64.56%), Postives = 61/79 (77.22%), Query Frame = 0


Query: 1   MGNDVDYRGQNFELLPFGAGRRSCPGMNMAVVAVELTLANLLLRFDWKLPDGMKEEDVDM 60
           M + VDYRGQ++ELLPFG+GRR CPGM M + AVEL L NLL  FDWKLPDGM  +D+D 
Sbjct: 421 MDSSVDYRGQHYELLPFGSGRRICPGMPMGIAAVELGLLNLLYFFDWKLPDGMTHKDIDT 480

Query: 61  EEDVGLTVTKKSPLKLVPL 80
           EE   LT+ KK PL+LVP+
Sbjct: 481 EEAGTLTIVKKVPLQLVPV 499

[Close]

BLAST of Bhi07G001454 vs. ExPASy Swiss-Prot
Match: P58050 (Cytochrome P450 71B13 OS=Arabidopsis thaliana OX=3702 GN=CYP71B13 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 1.1e-24
Identity = 49/79 (62.03%), Postives = 61/79 (77.22%), Query Frame = 0


Query: 1   MGNDVDYRGQNFELLPFGAGRRSCPGMNMAVVAVELTLANLLLRFDWKLPDGMKEEDVDM 60
           M N +DY+G NFELLPFG+GRR CPG+ M +  + LTL NLL RFDWKLP+GM+ EDVD+
Sbjct: 413 MDNQIDYKGLNFELLPFGSGRRICPGIGMGMALIHLTLINLLYRFDWKLPEGMEVEDVDL 472

Query: 61  EEDVGLTVTKKSPLKLVPL 80
           EE  GL   KK PL+L+P+
Sbjct: 473 EESYGLVCPKKVPLELIPV 491

[Close]

BLAST of Bhi07G001454 vs. ExPASy Swiss-Prot
Match: P58049 (Cytochrome P450 71B11 OS=Arabidopsis thaliana OX=3702 GN=CYP71B11 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.8e-24
Identity = 49/79 (62.03%), Postives = 60/79 (75.95%), Query Frame = 0


Query: 1   MGNDVDYRGQNFELLPFGAGRRSCPGMNMAVVAVELTLANLLLRFDWKLPDGMKEEDVDM 60
           M N +DY+G NFE LPFG+GRR CPG+ M +  V LTL NLL RFDWKLP+GM+ EDVD+
Sbjct: 413 MDNQIDYKGLNFEFLPFGSGRRMCPGIGMGMALVHLTLINLLYRFDWKLPEGMEVEDVDL 472

Query: 61  EEDVGLTVTKKSPLKLVPL 80
           EE  GL   KK PL+L+P+
Sbjct: 473 EESYGLVCPKKVPLQLIPV 491

[Close]

BLAST of Bhi07G001454 vs. ExPASy Swiss-Prot
Match: Q9ZU07 (Cytochrome P450 71B12 OS=Arabidopsis thaliana OX=3702 GN=CYP71B12 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 5.3e-24
Identity = 49/79 (62.03%), Postives = 60/79 (75.95%), Query Frame = 0


Query: 1   MGNDVDYRGQNFELLPFGAGRRSCPGMNMAVVAVELTLANLLLRFDWKLPDGMKEEDVDM 60
           M + +DY+G NFELLPFG+GRR CPG+ M +  V LTL NLL RFDWKLP+GMK  DVD+
Sbjct: 413 MDSQIDYKGLNFELLPFGSGRRICPGIGMGMALVHLTLINLLYRFDWKLPEGMKVADVDL 472

Query: 61  EEDVGLTVTKKSPLKLVPL 80
           EE  GL   KK PL+L+P+
Sbjct: 473 EESYGLVCPKKIPLQLIPV 491

[Close]

BLAST of Bhi07G001454 vs. ExPASy Swiss-Prot
Match: Q9LTM4 (Cytochrome P450 71B19 OS=Arabidopsis thaliana OX=3702 GN=CYP71B19 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 5.3e-24
Identity = 52/79 (65.82%), Postives = 61/79 (77.22%), Query Frame = 0


Query: 1   MGNDVDYRGQNFELLPFGAGRRSCPGMNMAVVAVELTLANLLLRFDWKLPDGMKEEDVDM 60
           M + VDYRGQ++ELLPFG+GRR CPGM M + AVEL L NLL  FDWKLPDGM  +D+D 
Sbjct: 421 MDSFVDYRGQHYELLPFGSGRRICPGMPMGIAAVELGLLNLLYFFDWKLPDGMTHKDIDT 480

Query: 61  EEDVGLTVTKKSPLKLVPL 80
           EE   LT+ KK PLKLVP+
Sbjct: 481 EEAGTLTIVKKVPLKLVPV 499

[Close]

BLAST of Bhi07G001454 vs. ExPASy Swiss-Prot
Match: Q9LTM3 (Cytochrome P450 71B20 OS=Arabidopsis thaliana OX=3702 GN=CYP71B20 PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 7.0e-24
Identity = 51/79 (64.56%), Postives = 61/79 (77.22%), Query Frame = 0


Query: 1   MGNDVDYRGQNFELLPFGAGRRSCPGMNMAVVAVELTLANLLLRFDWKLPDGMKEEDVDM 60
           M + VDYRGQ++ELLPFG+GRR CPGM M + AVEL L NLL  FDWKLPDGM  +D+D 
Sbjct: 421 MDSSVDYRGQHYELLPFGSGRRICPGMPMGIAAVELGLLNLLYFFDWKLPDGMTHKDIDT 480

Query: 61  EEDVGLTVTKKSPLKLVPL 80
           EE   LT+ KK PL+LVP+
Sbjct: 481 EEAGTLTIVKKVPLQLVPV 499

[Close]

BLAST of Bhi07G001454 vs. ExPASy TrEMBL
Match: A0A6J1DDC0 (cytochrome P450 71B34-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019732 PE=3 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.8e-30
Identity = 63/81 (77.78%), Postives = 74/81 (91.36%), Query Frame = 0


Query: 1   MGNDVDYRGQNFELLPFGAGRRSCPGMNMAVVAVELTLANLLLRFDWKLPDGMKEEDVDM 60
           +G+++D+RGQNFELLPFGAGRRSCPGM+MA VAVEL LAN+LL FDWKLP GMKEED+DM
Sbjct: 429 VGSNIDFRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDM 488

Query: 61  EEDVGLTVTKKSPLKLVPLNF 82
           EED GLTVTKKSPL L+P+ +
Sbjct: 489 EEDAGLTVTKKSPLHLIPVEY 509

[Close]

BLAST of Bhi07G001454 vs. ExPASy TrEMBL
Match: A0A6J1DEL1 (cytochrome P450 71B34-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019732 PE=3 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 5.2e-30
Identity = 62/81 (76.54%), Postives = 74/81 (91.36%), Query Frame = 0


Query: 1   MGNDVDYRGQNFELLPFGAGRRSCPGMNMAVVAVELTLANLLLRFDWKLPDGMKEEDVDM 60
           +G+++D+RGQNFELLPFGAGRRSCPGM+MA VAVEL LAN+LL FDWKLP GMKEED+D+
Sbjct: 429 VGSNIDFRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDV 488

Query: 61  EEDVGLTVTKKSPLKLVPLNF 82
           EED GLTVTKKSPL L+P+ +
Sbjct: 489 EEDAGLTVTKKSPLHLIPVEY 509

[Close]

BLAST of Bhi07G001454 vs. ExPASy TrEMBL
Match: A0A6J1KF27 (cytochrome P450 71B37-like OS=Cucurbita maxima OX=3661 GN=LOC111492661 PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.2e-29
Identity = 62/84 (73.81%), Postives = 75/84 (89.29%), Query Frame = 0


Query: 1   MGNDVDYRGQNFELLPFGAGRRSCPGMNMAVVAVELTLANLLLRFDWKLPDGMKEEDVDM 60
           +G+D++Y GQ+FELLPFGAGRR CPGMNM ++AVELTLANLLL FDWKLPDGMKEED+DM
Sbjct: 421 IGSDINYIGQHFELLPFGAGRRRCPGMNMGILAVELTLANLLLCFDWKLPDGMKEEDLDM 480

Query: 61  EEDVGLTVTKKSPLKLVPLNFSRV 85
           EE+ GLT+ K+SPLKL+P+   RV
Sbjct: 481 EENGGLTIPKRSPLKLIPIRHLRV 504

[Close]

BLAST of Bhi07G001454 vs. ExPASy TrEMBL
Match: A0A6J1KAW9 (cytochrome P450 71B37-like OS=Cucurbita maxima OX=3661 GN=LOC111492644 PE=3 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 3.7e-28
Identity = 60/79 (75.95%), Postives = 70/79 (88.61%), Query Frame = 0


Query: 1   MGNDVDYRGQNFELLPFGAGRRSCPGMNMAVVAVELTLANLLLRFDWKLPDGMKEEDVDM 60
           +G+++DY+GQNFELLPFG GRR CPGMNMA + VEL LANLL  FDWKLPDGMKEEDVDM
Sbjct: 429 IGSNIDYKGQNFELLPFGGGRRICPGMNMATLTVELALANLLWCFDWKLPDGMKEEDVDM 488

Query: 61  EEDVGLTVTKKSPLKLVPL 80
           EE+VGLT+ KKS LKL+P+
Sbjct: 489 EEEVGLTIAKKSLLKLIPV 507

[Close]

BLAST of Bhi07G001454 vs. ExPASy TrEMBL
Match: A0A6J1GWE9 (cytochrome P450 71B26-like OS=Cucurbita moschata OX=3662 GN=LOC111458144 PE=3 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 3.7e-28
Identity = 60/78 (76.92%), Postives = 70/78 (89.74%), Query Frame = 0


Query: 1   MGNDVDYRGQNFELLPFGAGRRSCPGMNMAVVAVELTLANLLLRFDWKLPDGMKEEDVDM 60
           +G+++DY+GQNFELLPFG GRR CPG+NMA + VEL LANLLL FDWKLPD MKEEDVDM
Sbjct: 430 IGSNIDYKGQNFELLPFGGGRRICPGINMATLTVELALANLLLCFDWKLPDEMKEEDVDM 489

Query: 61  EEDVGLTVTKKSPLKLVP 79
           EEDVGLTV +KSPL+L+P
Sbjct: 490 EEDVGLTVAQKSPLQLIP 507

The following BLAST results are available for this feature:

BLAST of Bhi07G001454 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G25140.1	7.6e-26	62.03	cytochrome P450, family 71, subfamily B, polypeptide 13	[more]
AT5G25120.1	1.3e-25	62.03	ytochrome p450, family 71, subfamily B, polypeptide 11	[more]
AT3G26170.1	3.8e-25	65.82	cytochrome P450, family 71, subfamily B, polypeptide 19	[more]
AT5G25130.1	3.8e-25	62.03	cytochrome P450, family 71, subfamily B, polypeptide 12	[more]
AT3G26180.1	4.9e-25	64.56	cytochrome P450, family 71, subfamily B, polypeptide 20	[more]

BLAST of Bhi07G001454 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P58050	1.1e-24	62.03	Cytochrome P450 71B13 OS=Arabidopsis thaliana OX=3702 GN=CYP71B13 PE=2 SV=1	[more]
P58049	1.8e-24	62.03	Cytochrome P450 71B11 OS=Arabidopsis thaliana OX=3702 GN=CYP71B11 PE=2 SV=1	[more]
Q9ZU07	5.3e-24	62.03	Cytochrome P450 71B12 OS=Arabidopsis thaliana OX=3702 GN=CYP71B12 PE=2 SV=1	[more]
Q9LTM4	5.3e-24	65.82	Cytochrome P450 71B19 OS=Arabidopsis thaliana OX=3702 GN=CYP71B19 PE=1 SV=1	[more]
Q9LTM3	7.0e-24	64.56	Cytochrome P450 71B20 OS=Arabidopsis thaliana OX=3702 GN=CYP71B20 PE=2 SV=1	[more]

BLAST of Bhi07G001454 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DDC0	1.8e-30	77.78	cytochrome P450 71B34-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A6J1DEL1	5.2e-30	76.54	cytochrome P450 71B34-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A6J1KF27	1.2e-29	73.81	cytochrome P450 71B37-like OS=Cucurbita maxima OX=3661 GN=LOC111492661 PE=3 SV=1	[more]
A0A6J1KAW9	3.7e-28	75.95	cytochrome P450 71B37-like OS=Cucurbita maxima OX=3661 GN=LOC111492644 PE=3 SV=1	[more]
A0A6J1GWE9	3.7e-28	76.92	cytochrome P450 71B26-like OS=Cucurbita moschata OX=3662 GN=LOC111458144 PE=3 SV...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 14..24 score: 59.17 coord: 24..47 score: 33.06
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 9..70 e-value: 3.6E-15 score: 55.7
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 2..80 e-value: 1.9E-18 score: 68.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 6..78
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 3..79
None	No IPR available	PANTHER	PTHR24298:SF379	OS08G0105800 PROTEIN	coord: 3..79
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 17..26

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi07M001454	Bhi07M001454	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen