Bhi07G001406 (gene) Wax gourd (B227) v1

Overview
NameBhi07G001406
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionhigh affinity nitrate transporter 2.5
Locationchr7: 48201267 .. 48201614 (-)
RNA-Seq ExpressionBhi07G001406
SyntenyBhi07G001406
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAGGTGCGGGTGGGAACATGGGATCGGTGCTGACTCAGCTGATATTCTTCAGAGGCAACAAATACAGAAAAGAGAGTGGAATAACTCTAATGGGAGTGATGATAATAGCTTGCACTCTTCCAATTGTATTGATTTATTTTCCTCAATGGGGCAGTATGTTATTTGGGCCTTCCTCCAAAATTGCTTCAGAGGAAGATTATTACTTGTCCGAATGGAGCTCTGAGGAGAAAGCCGAAGGCTATCATATGGCTAGCCTTAAGTTTGCTGACAATAGTCGAACTGAAAGAAGCAAAAGCCTTAAAGGAACTGCTCATGCCGATGATTCCTCCTCCACTCATGTTTAA

mRNA sequence

ATGACAGGTGCGGGTGGGAACATGGGATCGGTGCTGACTCAGCTGATATTCTTCAGAGGCAACAAATACAGAAAAGAGAGTGGAATAACTCTAATGGGAGTGATGATAATAGCTTGCACTCTTCCAATTGTATTGATTTATTTTCCTCAATGGGGCAGTATGTTATTTGGGCCTTCCTCCAAAATTGCTTCAGAGGAAGATTATTACTTGTCCGAATGGAGCTCTGAGGAGAAAGCCGAAGGCTATCATATGGCTAGCCTTAAGTTTGCTGACAATAGTCGAACTGAAAGAAGCAAAAGCCTTAAAGGAACTGCTCATGCCGATGATTCCTCCTCCACTCATGTTTAA

Coding sequence (CDS)

ATGACAGGTGCGGGTGGGAACATGGGATCGGTGCTGACTCAGCTGATATTCTTCAGAGGCAACAAATACAGAAAAGAGAGTGGAATAACTCTAATGGGAGTGATGATAATAGCTTGCACTCTTCCAATTGTATTGATTTATTTTCCTCAATGGGGCAGTATGTTATTTGGGCCTTCCTCCAAAATTGCTTCAGAGGAAGATTATTACTTGTCCGAATGGAGCTCTGAGGAGAAAGCCGAAGGCTATCATATGGCTAGCCTTAAGTTTGCTGACAATAGTCGAACTGAAAGAAGCAAAAGCCTTAAAGGAACTGCTCATGCCGATGATTCCTCCTCCACTCATGTTTAA

Protein sequence

MTGAGGNMGSVLTQLIFFRGNKYRKESGITLMGVMIIACTLPIVLIYFPQWGSMLFGPSSKIASEEDYYLSEWSSEEKAEGYHMASLKFADNSRTERSKSLKGTAHADDSSSTHV
Homology
[Close]
BLAST of Bhi07G001406 vs. TAIR 10
Match: AT1G12940.1 (nitrate transporter2.5 )

HSP 1 Score: 139.4 bits (350), Expect = 1.8e-33
Identity = 66/100 (66.00%), Postives = 82/100 (82.00%), Query Frame = 0 

Query: 1   MTGAGGNMGSVLTQLIFFRGNKYRKESGITLMGVMIIACTLPIVLIYFPQWGSMLFGPSS 60
           MTGAGGN+G+VLTQLIFF+G+ Y +E+GITLMGVM IAC+LPI LIYFPQWG M  GPSS
Sbjct: 396 MTGAGGNVGAVLTQLIFFKGSTYTRETGITLMGVMSIACSLPICLIYFPQWGGMFCGPSS 455

Query: 61  KIASEEDYYLSEWSSEEKAEGYHMASLKFADNSRTERSKS 101
           K  +EEDYYL+EW+ EEK +  H+ S KFA+ S +ER ++
Sbjct: 456 KKVTEEDYYLAEWNDEEKEKNLHIGSQKFAETSISERGRA 495

[Close]
BLAST of Bhi07G001406 vs. TAIR 10
Match: AT1G08100.1 (nitrate transporter 2.2 )

HSP 1 Score: 114.4 bits (285), Expect = 6.1e-26
Identity = 58/107 (54.21%), Postives = 77/107 (71.96%), Query Frame = 0 

Query: 1   MTGAGGNMGSVLTQLIFFRGNKYRKESGITLMGVMIIACTLPIVLIYFPQWGSMLFGPSS 60
           +TGAGGN GS LTQL+FF  +++  E G+T MGVMI+ACTLP+ LI+FPQWGSM F PS+
Sbjct: 412 LTGAGGNFGSGLTQLVFFSTSRFTTEEGLTWMGVMIVACTLPVTLIHFPQWGSMFFPPSN 471

Query: 61  -KIASEEDYYLSEWSSEEKAEGYHMASLKFADNSRTERSKSL-KGTA 106
             + + E YY+ E+S EE+  G H+ S  FAD ++TE   S+ KG A
Sbjct: 472 DSVDATEHYYVGEYSKEEQQIGMHLKSKLFADGAKTEGGSSVHKGNA 518

[Close]
BLAST of Bhi07G001406 vs. TAIR 10
Match: AT5G60770.1 (nitrate transporter 2.4 )

HSP 1 Score: 114.4 bits (285), Expect = 6.1e-26
Identity = 58/102 (56.86%), Postives = 76/102 (74.51%), Query Frame = 0 

Query: 1   MTGAGGNMGSVLTQLIFFRGNKYRKESGITLMGVMIIACTLPIVLIYFPQWGSMLFGPSS 60
           +TGAGGN GS LTQL+FF  + +  E G+T MGVMI+ACTLP+ L++FPQWGSM F PS+
Sbjct: 414 LTGAGGNFGSGLTQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTLVHFPQWGSM-FLPST 473

Query: 61  K---IASEEDYYLSEWSSEEKAEGYHMASLKFADNSRTERSK 100
           +    ++EE YY+ EW+  EK +G H  SLKFA NSR+ER +
Sbjct: 474 EDEVKSTEEYYYMKEWTETEKRKGMHEGSLKFAVNSRSERGR 514

[Close]
BLAST of Bhi07G001406 vs. TAIR 10
Match: AT3G45060.1 (high affinity nitrate transporter 2.6 )

HSP 1 Score: 112.1 bits (279), Expect = 3.0e-25
Identity = 52/101 (51.49%), Postives = 73/101 (72.28%), Query Frame = 0 

Query: 1   MTGAGGNMGSVLTQLIFFRGNKYRKESGITLMGVMIIACTLPIVLIYFPQWGSMLFGPS- 60
           +TGAGGN GS +TQL+FF  +++    G++LMGVM + C+LP+  I+FPQWGSM   PS 
Sbjct: 424 LTGAGGNFGSGVTQLLFFSSSRFSTAEGLSLMGVMAVVCSLPVAFIHFPQWGSMFLRPSQ 483

Query: 61  -SKIASEEDYYLSEWSSEEKAEGYHMASLKFADNSRTERSK 100
             + + EE YY +EW+ EEK+ G H  S+KFA+NSR+ER +
Sbjct: 484 DGEKSKEEHYYGAEWTEEEKSLGLHEGSIKFAENSRSERGR 524

[Close]
BLAST of Bhi07G001406 vs. TAIR 10
Match: AT1G08090.1 (nitrate transporter 2:1 )

HSP 1 Score: 111.7 bits (278), Expect = 4.0e-25
Identity = 54/117 (46.15%), Postives = 80/117 (68.38%), Query Frame = 0 

Query: 1   MTGAGGNMGSVLTQLIFFRGNKYRKESGITLMGVMIIACTLPIVLIYFPQWGSMLFGPSS 60
           +TGAGGN GS LTQL+FF  + +  E G+T MGVMI+ACTLP+ L++FPQWGSM   PS+
Sbjct: 414 LTGAGGNFGSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPST 473

Query: 61  K--IASEEDYYLSEWSSEEKAEGYHMASLKFADNSRTERSKSLKGTAHADDSSSTHV 116
                +E  YY SEW+ +EK +  H  SL+FA+N+++E  + ++  A   +++  +V
Sbjct: 474 DPVKGTEAHYYGSEWNEQEKQKNMHQGSLRFAENAKSEGGRRVRSAATPPENTPNNV 530

[Close]
BLAST of Bhi07G001406 vs. ExPASy Swiss-Prot
Match: Q9LPV5 (High affinity nitrate transporter 2.5 OS=Arabidopsis thaliana OX=3702 GN=NRT2.5 PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 2.5e-32
Identity = 66/100 (66.00%), Postives = 82/100 (82.00%), Query Frame = 0 

Query: 1   MTGAGGNMGSVLTQLIFFRGNKYRKESGITLMGVMIIACTLPIVLIYFPQWGSMLFGPSS 60
           MTGAGGN+G+VLTQLIFF+G+ Y +E+GITLMGVM IAC+LPI LIYFPQWG M  GPSS
Sbjct: 396 MTGAGGNVGAVLTQLIFFKGSTYTRETGITLMGVMSIACSLPICLIYFPQWGGMFCGPSS 455

Query: 61  KIASEEDYYLSEWSSEEKAEGYHMASLKFADNSRTERSKS 101
           K  +EEDYYL+EW+ EEK +  H+ S KFA+ S +ER ++
Sbjct: 456 KKVTEEDYYLAEWNDEEKEKNLHIGSQKFAETSISERGRA 495

[Close]
BLAST of Bhi07G001406 vs. ExPASy Swiss-Prot
Match: P0DKG9 (High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.1e-27
Identity = 59/99 (59.60%), Postives = 78/99 (78.79%), Query Frame = 0 

Query: 1   MTGAGGNMGSVLTQLIFFRGNKYRKESGITLMGVMIIACTLPIVLIYFPQWGSMLFGPSS 60
           MTGAGGN G+ LTQL+FF  ++Y   +G+  MG+MI+ACTLP+VL++FPQWGSM   P++
Sbjct: 415 MTGAGGNFGAGLTQLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNA 474

Query: 61  KIASEEDYYLSEWSSEEKAEGYHMASLKFADNSRTERSK 100
             A EE YY SEWS +EK++G H ASLKFA+NSR+ER +
Sbjct: 475 G-AEEEHYYGSEWSEQEKSKGLHGASLKFAENSRSERGR 512

[Close]
BLAST of Bhi07G001406 vs. ExPASy Swiss-Prot
Match: P0DKH0 (High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.2 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.1e-27
Identity = 59/99 (59.60%), Postives = 78/99 (78.79%), Query Frame = 0 

Query: 1   MTGAGGNMGSVLTQLIFFRGNKYRKESGITLMGVMIIACTLPIVLIYFPQWGSMLFGPSS 60
           MTGAGGN G+ LTQL+FF  ++Y   +G+  MG+MI+ACTLP+VL++FPQWGSM   P++
Sbjct: 415 MTGAGGNFGAGLTQLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNA 474

Query: 61  KIASEEDYYLSEWSSEEKAEGYHMASLKFADNSRTERSK 100
             A EE YY SEWS +EK++G H ASLKFA+NSR+ER +
Sbjct: 475 G-AEEEHYYGSEWSEQEKSKGLHGASLKFAENSRSERGR 512

[Close]
BLAST of Bhi07G001406 vs. ExPASy Swiss-Prot
Match: Q94JG1 (High-affinity nitrate transporter 2.3 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.3 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.1e-27
Identity = 60/100 (60.00%), Postives = 77/100 (77.00%), Query Frame = 0 

Query: 1   MTGAGGNMGSVLTQLIFFRGNKYRKESGITLMGVMIIACTLPIVLIYFPQWGSMLFGPSS 60
           MTG GGN+G+VLTQ IFF G KY+ E+GI  MG+MIIACTLP++LIYFPQWG ML GP  
Sbjct: 398 MTGGGGNVGAVLTQYIFFHGTKYKTETGIKYMGLMIIACTLPVMLIYFPQWGGMLVGP-R 457

Query: 61  KIASEEDYYLSEWSSEEKAEGYHMASLKFADNSRTERSKS 101
           K A+ E+YY  EWS  E+ +G++ AS++FA+NS  E  +S
Sbjct: 458 KGATAEEYYSREWSDHEREKGFNAASVRFAENSVREGGRS 496

[Close]
BLAST of Bhi07G001406 vs. ExPASy Swiss-Prot
Match: Q9LMZ9 (High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana OX=3702 GN=NRT2.2 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 8.6e-25
Identity = 58/107 (54.21%), Postives = 77/107 (71.96%), Query Frame = 0 

Query: 1   MTGAGGNMGSVLTQLIFFRGNKYRKESGITLMGVMIIACTLPIVLIYFPQWGSMLFGPSS 60
           +TGAGGN GS LTQL+FF  +++  E G+T MGVMI+ACTLP+ LI+FPQWGSM F PS+
Sbjct: 412 LTGAGGNFGSGLTQLVFFSTSRFTTEEGLTWMGVMIVACTLPVTLIHFPQWGSMFFPPSN 471

Query: 61  -KIASEEDYYLSEWSSEEKAEGYHMASLKFADNSRTERSKSL-KGTA 106
             + + E YY+ E+S EE+  G H+ S  FAD ++TE   S+ KG A
Sbjct: 472 DSVDATEHYYVGEYSKEEQQIGMHLKSKLFADGAKTEGGSSVHKGNA 518

[Close]
BLAST of Bhi07G001406 vs. ExPASy TrEMBL
Match: A0A5A7SWP7 (High affinity nitrate transporter 2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001200 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.9e-46
Identity = 96/115 (83.48%), Postives = 104/115 (90.43%), Query Frame = 0 

Query: 1   MTGAGGNMGSVLTQLIFFRGNKYRKESGITLMGVMIIACTLPIVLIYFPQWGSMLFGPSS 60
           MTG GGN+GSVLTQLIFFRGN+Y KE+GITLMGVMIIACTLPIVLIYFPQWGSML GPSS
Sbjct: 391 MTGGGGNVGSVLTQLIFFRGNRYTKETGITLMGVMIIACTLPIVLIYFPQWGSMLLGPSS 450

Query: 61  KIASEEDYYLSEWSSEEKAEGYHMASLKFADNSRTERSKSLKGTAHADDSSSTHV 116
           KIASEEDYYLSEWS EEK +GYH+ SLKFA+NSR+ER K LKGTA ADD S +HV
Sbjct: 451 KIASEEDYYLSEWSDEEKTKGYHIGSLKFAENSRSERGKPLKGTADADDDSPSHV 505

[Close]
BLAST of Bhi07G001406 vs. ExPASy TrEMBL
Match: A0A1S3BDY8 (high affinity nitrate transporter 2.5 OS=Cucumis melo OX=3656 GN=LOC103488988 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.9e-46
Identity = 96/115 (83.48%), Postives = 104/115 (90.43%), Query Frame = 0 

Query: 1   MTGAGGNMGSVLTQLIFFRGNKYRKESGITLMGVMIIACTLPIVLIYFPQWGSMLFGPSS 60
           MTG GGN+GSVLTQLIFFRGN+Y KE+GITLMGVMIIACTLPIVLIYFPQWGSML GPSS
Sbjct: 391 MTGGGGNVGSVLTQLIFFRGNRYTKETGITLMGVMIIACTLPIVLIYFPQWGSMLLGPSS 450

Query: 61  KIASEEDYYLSEWSSEEKAEGYHMASLKFADNSRTERSKSLKGTAHADDSSSTHV 116
           KIASEEDYYLSEWS EEK +GYH+ SLKFA+NSR+ER K LKGTA ADD S +HV
Sbjct: 451 KIASEEDYYLSEWSDEEKTKGYHIGSLKFAENSRSERGKPLKGTADADDDSPSHV 505

[Close]
BLAST of Bhi07G001406 vs. ExPASy TrEMBL
Match: S4VIV8 (High-affinity nitrate transporter NRT2.3 OS=Cucumis sativus OX=3659 GN=NRT2.3 PE=2 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.4e-46
Identity = 97/115 (84.35%), Postives = 102/115 (88.70%), Query Frame = 0 

Query: 1   MTGAGGNMGSVLTQLIFFRGNKYRKESGITLMGVMIIACTLPIVLIYFPQWGSMLFGPSS 60
           MTG GGN+GSVLTQLIFFRGN+Y KE GITLMGVMIIACTLPIVLIYFPQWGSML GPSS
Sbjct: 385 MTGGGGNVGSVLTQLIFFRGNRYTKERGITLMGVMIIACTLPIVLIYFPQWGSMLLGPSS 444

Query: 61  KIASEEDYYLSEWSSEEKAEGYHMASLKFADNSRTERSKSLKGTAHADDSSSTHV 116
           KIASEEDYYLSEWS EEK +GYHM SLKFA+NSR ER K+LKGTA ADD S  HV
Sbjct: 445 KIASEEDYYLSEWSDEEKNKGYHMGSLKFAENSRNERGKTLKGTADADDDSPPHV 499

[Close]
BLAST of Bhi07G001406 vs. ExPASy TrEMBL
Match: A0A6J1H2D0 (high affinity nitrate transporter 2.5 OS=Cucurbita moschata OX=3662 GN=LOC111458928 PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 5.2e-41
Identity = 95/118 (80.51%), Postives = 105/118 (88.98%), Query Frame = 0 

Query: 1   MTGAGGNMGSVLTQLIFFRGNKYRKESGITLMGVMIIACTLPIVLIYFPQWGSMLFGP-S 60
           MTGAGGN+GSVLTQLIFFRG++Y KE GI+LMGVMII CTLPIVLIYFPQWGSML GP S
Sbjct: 390 MTGAGGNVGSVLTQLIFFRGSRYSKEKGISLMGVMIIGCTLPIVLIYFPQWGSMLTGPAS 449

Query: 61  SKIASEEDYYLSEWSSEEKAEGYHMASLKFADNSRTERSKSL-KGTAH-ADDSSSTHV 116
           SKIASEE+YYLSEW+ EEKA+GYH+A+LKFADNSRTER KSL KGTA  A  S+S HV
Sbjct: 450 SKIASEEEYYLSEWTPEEKAKGYHLAALKFADNSRTERGKSLSKGTADAASASASPHV 507

[Close]
BLAST of Bhi07G001406 vs. ExPASy TrEMBL
Match: A0A6J1JZK1 (high affinity nitrate transporter 2.5 OS=Cucurbita maxima OX=3661 GN=LOC111489712 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 3.2e-38
Identity = 89/119 (74.79%), Postives = 102/119 (85.71%), Query Frame = 0 

Query: 1   MTGAGGNMGSVLTQLIFFRGNKYRKESGITLMGVMIIACTLPIVLIYFPQWGSMLFGP-S 60
           MTGAGGN+GSVLTQLIFFRG++Y KE GI+LMGVMII CTLPI+LIYFPQWG ML GP S
Sbjct: 390 MTGAGGNVGSVLTQLIFFRGSRYSKEKGISLMGVMIIGCTLPILLIYFPQWGGMLTGPAS 449

Query: 61  SKIASEEDYYLSEWSSEEKAEGYHMASLKFADNSRTERSKSL-KGTAHADD--SSSTHV 116
           SKIASEE+YYLSEW+ EE+ +GYH+A+LKFA NSRTER KSL KGTA A    ++S HV
Sbjct: 450 SKIASEEEYYLSEWTPEERVKGYHLAALKFAQNSRTERGKSLSKGTADAASAAAASPHV 508

The following BLAST results are available for this feature:
BLAST of Bhi07G001406 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G12940.11.8e-3366.00nitrate transporter2.5 [more]
AT1G08100.16.1e-2654.21nitrate transporter 2.2 [more]
AT5G60770.16.1e-2656.86nitrate transporter 2.4 [more]
AT3G45060.13.0e-2551.49high affinity nitrate transporter 2.6 [more]
AT1G08090.14.0e-2546.15nitrate transporter 2:1 [more]
BLAST of Bhi07G001406 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LPV52.5e-3266.00High affinity nitrate transporter 2.5 OS=Arabidopsis thaliana OX=3702 GN=NRT2.5 ... [more]
P0DKG91.1e-2759.60High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
P0DKH01.1e-2759.60High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q94JG11.1e-2760.00High-affinity nitrate transporter 2.3 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9LMZ98.6e-2554.21High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana OX=3702 GN=NRT2.2 ... [more]
BLAST of Bhi07G001406 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7SWP71.9e-4683.48High affinity nitrate transporter 2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BDY81.9e-4683.48high affinity nitrate transporter 2.5 OS=Cucumis melo OX=3656 GN=LOC103488988 PE... [more]
S4VIV82.4e-4684.35High-affinity nitrate transporter NRT2.3 OS=Cucumis sativus OX=3659 GN=NRT2.3 PE... [more]
A0A6J1H2D05.2e-4180.51high affinity nitrate transporter 2.5 OS=Cucurbita moschata OX=3662 GN=LOC111458... [more]
A0A6J1JZK13.2e-3874.79high affinity nitrate transporter 2.5 OS=Cucurbita maxima OX=3661 GN=LOC11148971... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 93..115
NoneNo IPR availablePANTHERPTHR23515:SF13HIGH-AFFINITY NITRATE TRANSPORTER 2.3-LIKEcoord: 1..100
IPR044772Nitrate transporterPANTHERPTHR23515HIGH-AFFINITY NITRATE TRANSPORTER 2.3coord: 1..100

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi07M001406Bhi07M001406mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071249 cellular response to nitrate
biological_process GO:0042128 nitrate assimilation
biological_process GO:0015706 nitrate transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0015112 nitrate transmembrane transporter activity