Bhi07G001386 (gene) Wax gourd (B227) v1

Overview
NameBhi07G001386
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionhybrid signal transduction histidine kinase L
Locationchr7: 47829447 .. 47830794 (-)
RNA-Seq ExpressionBhi07G001386
SyntenyBhi07G001386
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCCAACAATATAATCATCAATCAAAATACCACTTCTTCTCTTCATTAAAACTCAAAATTTAAAAGAACTACCTCAAAGAATATGTTTAGCTGTCTGTCTCTTTACCAAAAGAAAGTTCCTCCCTTTTTGACCTCTTTTTTTTCTATCCTCTCTTAAATCCCATTTTTTAGAATCCCATTCATTATACATAACCCTCTTCCCTATGCAACCTTCTTGCTTCTTTCTTCAGTAAATTCCATATCTCTCCCTTCTCTTCCACTCAAAGCACCACCTCCATATTTCATATCAGCTTCTTAATAGCCTGAAATACCAATCCCCCACTTCCTTAATTTCTCCTAAATTCAAATTCTCATTAACCTTAGCCGACATGTCGGAACTTCAACAGCAACAGCAACAGCAACCGCAACAGCAACAGCAGCAGCAGCCGGCTTTTGTTTATCCCAACACCGCCGCGACGGTCGGCAGCCAACCTCCTCCTTCCCATCATTCCAACGGCTCATTTGGGGCAGTTTTTATAGTTCTTGCCGTTATTGTTGTCATATCGGCTCTTGCTTGTTTCCTTGGCCGTCTGTGTAACCGGCGAGTTGAAAACGGCCAGAGGCCGAAGCCGGAGAAAACCCACAGCGGCGCTCGTCCTAGAGGAAAAGAACCCAAATTGCATCCTGATAAAGAAGGAGACATTGAATTTGGATTTGATTTGAGAAACCCAAATGGGAACAAAGGCAAATTCCCATCTTCTGGTGGAAATGGAGGAAGCAAAGGGAAAAGCAATGGGAATGGTAATGGGACTAATGGGCCTAACGGCCATGGAAATAGCTTCAAACCCTTTGAAAATGGCGGTGAAACGAGGAGTGAAATCAAGCATACTGATCATCATGAAGGGGATTTCAATTTCAAAGCTGCAGGGCATCATGTTTGATGGGTACTAATAAAACAACATAGAAAAATGAAAATGCCCAATTTCATCGTATCAAGTTATAACAGTGTGGATTTTTTCTTTTTCCTCTGTTTTTTTCTCTTTTCACTTGATGTTTGTTCTAATCTAATGAGTAATGACAAGCTTCAATTCAATCATCAATCTTTGATGTTATTCAATGGATGCTGTTGAATTCAAGAGTAGGAATCTTAAGTAGTTGAAATCTTTTTGATCGTATATCTTCAAGCTCACGGCAACTTCAATTAACAGATGTTTTTGGGTACTTTTGTGTGTTAAAAGTAATGGAAGAATGAGGCTTCGAATAAAGCCAGAGAATATGATTCCGAATAATGGAGGTGGGAGAACTCGAACTCTTATTAAAGCGGATTCATTAAGGATGTTTGATAAATGAGTATGAGTTGTTGAAGTGG

mRNA sequence

GTCCAACAATATAATCATCAATCAAAATACCACTTCTTCTCTTCATTAAAACTCAAAATTTAAAAGAACTACCTCAAAGAATATGTTTAGCTGTCTGTCTCTTTACCAAAAGAAAGTTCCTCCCTTTTTGACCTCTTTTTTTTCTATCCTCTCTTAAATCCCATTTTTTAGAATCCCATTCATTATACATAACCCTCTTCCCTATGCAACCTTCTTGCTTCTTTCTTCAGTAAATTCCATATCTCTCCCTTCTCTTCCACTCAAAGCACCACCTCCATATTTCATATCAGCTTCTTAATAGCCTGAAATACCAATCCCCCACTTCCTTAATTTCTCCTAAATTCAAATTCTCATTAACCTTAGCCGACATGTCGGAACTTCAACAGCAACAGCAACAGCAACCGCAACAGCAACAGCAGCAGCAGCCGGCTTTTGTTTATCCCAACACCGCCGCGACGGTCGGCAGCCAACCTCCTCCTTCCCATCATTCCAACGGCTCATTTGGGGCAGTTTTTATAGTTCTTGCCGTTATTGTTGTCATATCGGCTCTTGCTTGTTTCCTTGGCCGTCTGTGTAACCGGCGAGTTGAAAACGGCCAGAGGCCGAAGCCGGAGAAAACCCACAGCGGCGCTCGTCCTAGAGGAAAAGAACCCAAATTGCATCCTGATAAAGAAGGAGACATTGAATTTGGATTTGATTTGAGAAACCCAAATGGGAACAAAGGCAAATTCCCATCTTCTGGTGGAAATGGAGGAAGCAAAGGGAAAAGCAATGGGAATGGTAATGGGACTAATGGGCCTAACGGCCATGGAAATAGCTTCAAACCCTTTGAAAATGGCGGTGAAACGAGGAGTGAAATCAAGCATACTGATCATCATGAAGGGGATTTCAATTTCAAAGCTGCAGGGCATCATGTTTGATGGGTACTAATAAAACAACATAGAAAAATGAAAATGCCCAATTTCATCGTATCAAGTTATAACAGTGTGGATTTTTTCTTTTTCCTCTGTTTTTTTCTCTTTTCACTTGATGTTTGTTCTAATCTAATGAGTAATGACAAGCTTCAATTCAATCATCAATCTTTGATGTTATTCAATGGATGCTGTTGAATTCAAGAGTAGGAATCTTAAGTAGTTGAAATCTTTTTGATCGTATATCTTCAAGCTCACGGCAACTTCAATTAACAGATGTTTTTGGGTACTTTTGTGTGTTAAAAGTAATGGAAGAATGAGGCTTCGAATAAAGCCAGAGAATATGATTCCGAATAATGGAGGTGGGAGAACTCGAACTCTTATTAAAGCGGATTCATTAAGGATGTTTGATAAATGAGTATGAGTTGTTGAAGTGG

Coding sequence (CDS)

ATGTCGGAACTTCAACAGCAACAGCAACAGCAACCGCAACAGCAACAGCAGCAGCAGCCGGCTTTTGTTTATCCCAACACCGCCGCGACGGTCGGCAGCCAACCTCCTCCTTCCCATCATTCCAACGGCTCATTTGGGGCAGTTTTTATAGTTCTTGCCGTTATTGTTGTCATATCGGCTCTTGCTTGTTTCCTTGGCCGTCTGTGTAACCGGCGAGTTGAAAACGGCCAGAGGCCGAAGCCGGAGAAAACCCACAGCGGCGCTCGTCCTAGAGGAAAAGAACCCAAATTGCATCCTGATAAAGAAGGAGACATTGAATTTGGATTTGATTTGAGAAACCCAAATGGGAACAAAGGCAAATTCCCATCTTCTGGTGGAAATGGAGGAAGCAAAGGGAAAAGCAATGGGAATGGTAATGGGACTAATGGGCCTAACGGCCATGGAAATAGCTTCAAACCCTTTGAAAATGGCGGTGAAACGAGGAGTGAAATCAAGCATACTGATCATCATGAAGGGGATTTCAATTTCAAAGCTGCAGGGCATCATGTTTGA

Protein sequence

MSELQQQQQQQPQQQQQQQPAFVYPNTAATVGSQPPPSHHSNGSFGAVFIVLAVIVVISALACFLGRLCNRRVENGQRPKPEKTHSGARPRGKEPKLHPDKEGDIEFGFDLRNPNGNKGKFPSSGGNGGSKGKSNGNGNGTNGPNGHGNSFKPFENGGETRSEIKHTDHHEGDFNFKAAGHHV
Homology
BLAST of Bhi07G001386 vs. TAIR 10
Match: AT1G23530.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G70470.1); Has 38 Blast hits to 38 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 37; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 70.9 bits (172), Expect = 1.2e-12
Identity = 66/175 (37.71%), Postives = 85/175 (48.57%), Query Frame = 0

Query: 15  QQQQQPAFVYPNT----AATVGSQPPPSHHSNGSFGAVFIVLAVIVVISALACFLGRLCN 74
           QQ QQP  VYPN          ++P  S  S+G+FG VFIVLAVI V+SALAC  GRLCN
Sbjct: 7   QQMQQPIVVYPNAYNKQYPDPSTKPSSSSGSSGNFGTVFIVLAVIFVLSALACVFGRLCN 66

Query: 75  R-----RVENGQRPKPEKTHSGARPRGKEPKLHPDKEGDIEFGFDLRNPNGNKGKFPSSG 134
           R     + ++ + PK EK  S      +  +  P + GD++FGFD++ P   +      G
Sbjct: 67  RESRAAKQQHSKHPKHEKASSKKSREIRPVEREPRERGDMDFGFDMKRPEPIEKPSGRDG 126

Query: 135 GNG---------GSKGKSNGNGNGTNGPN-GHGNSFKPFENGGETRSEIKHTDHH 171
           G           G +G+  G G G   PN  H   FK  ENG          DHH
Sbjct: 127 GEDIEFGFDNKRGERGREGGGGGGPPPPNIKHAVRFKLPENG----------DHH 171

BLAST of Bhi07G001386 vs. TAIR 10
Match: AT1G70470.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G23530.1); Has 64 Blast hits to 64 proteins in 22 species: Archae - 0; Bacteria - 2; Metazoa - 7; Fungi - 10; Plants - 43; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 63.2 bits (152), Expect = 2.6e-10
Identity = 58/145 (40.00%), Postives = 76/145 (52.41%), Query Frame = 0

Query: 11  QPQQQQQQQPAFVYPNTAATVGSQ-PPPSHHSNGSFGAVFIVLAVIVVISALACFLGRLC 70
           QPQ+ QQ Q   VYP+T+++  S   P S  S+GSFG  FIVLA I+V++ALAC  GRLC
Sbjct: 4   QPQEFQQPQ---VYPSTSSSTSSSFSPSSSGSSGSFGTAFIVLAAILVLAALACVFGRLC 63

Query: 71  NR------RVENGQRPKPEKTHSGARPRGKEPKLHPDKEGDIEFGFDLRNPNGNKGKFPS 130
           NR         N +  K EK  S      +  +  P + GD+EFGFD++ P         
Sbjct: 64  NRGGKQHKDKNNNKASKHEKPSSKKSREIRPVEREPRERGDVEFGFDMKRPGPMSKPTGR 123

Query: 131 SGGN--GGSKGKSNGNGNGTNGPNG 147
           +GG+   G   K  G G G  G  G
Sbjct: 124 NGGDIEFGFDNKRGGGGGGGGGGKG 145

BLAST of Bhi07G001386 vs. TAIR 10
Match: AT1G74055.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 15 plant structures; EXPRESSED DURING: 6 growth stages; Has 24 Blast hits to 24 proteins in 9 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 24; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 43.9 bits (102), Expect = 1.6e-04
Identity = 24/63 (38.10%), Postives = 37/63 (58.73%), Query Frame = 0

Query: 9  QQQPQQQQQQQPAFVYPNTAATVGSQPPPSHHSNGSFGAVFIVLAVIVVISALACFLGRL 68
          QQQPQQQ            A  + +   P+H+S+ S G  F V++V+++++ L+CFLGR 
Sbjct: 16 QQQPQQQLGGNEITPMATNANLIAAPNAPNHYSSSSIGPFFAVISVLIILAVLSCFLGRF 75

Query: 69 CNR 72
          C R
Sbjct: 76 CAR 78

BLAST of Bhi07G001386 vs. ExPASy TrEMBL
Match: A0A5A7ST81 (Hybrid signal transduction histidine kinase L OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001050 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 2.2e-73
Identity = 165/191 (86.39%), Postives = 171/191 (89.53%), Query Frame = 0

Query: 1   MSELQQQQQQ-QPQQQQQQQPAFVYPNTAAT-VGSQPPPSHHSNGSFGAVFIVLAVIVVI 60
           MSELQQQQ+Q Q QQQQQQQPA+VYP+TAAT  GSQ PPSHHSNGSFGAVFIVLAVIVVI
Sbjct: 1   MSELQQQQEQPQQQQQQQQQPAYVYPDTAATSAGSQVPPSHHSNGSFGAVFIVLAVIVVI 60

Query: 61  SALACFLGRLCNRRVENGQRPKPEKTHSGARP-RGKEPKLHPDKEGDIEFGFDLRNPNGN 120
           SALACFLGRLCNRRVEN QRPKPEK+H+GARP RGKEPKLHPDKEGDIEFGFDLRNP+G 
Sbjct: 61  SALACFLGRLCNRRVENHQRPKPEKSHAGARPTRGKEPKLHPDKEGDIEFGFDLRNPSG- 120

Query: 121 KGKFPSSG-----GNGGSKGKSNGNGN-GTNGPNGHGNSFKPFENGGETRSEIKHTDHHE 180
           KGKFP  G     GNGGSKGK NGNG  G NGPNGHGNSFKPFENGGETRSEIKHTDHHE
Sbjct: 121 KGKFPPGGNGNGNGNGGSKGKGNGNGPIGLNGPNGHGNSFKPFENGGETRSEIKHTDHHE 180

Query: 181 GDFNFKAAGHH 183
           GDFNFKA GHH
Sbjct: 181 GDFNFKAGGHH 190

BLAST of Bhi07G001386 vs. ExPASy TrEMBL
Match: E5GBJ1 (Uncharacterized protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 2.2e-73
Identity = 165/191 (86.39%), Postives = 171/191 (89.53%), Query Frame = 0

Query: 1   MSELQQQQQQ-QPQQQQQQQPAFVYPNTAAT-VGSQPPPSHHSNGSFGAVFIVLAVIVVI 60
           MSELQQQQ+Q Q QQQQQQQPA+VYP+TAAT  GSQ PPSHHSNGSFGAVFIVLAVIVVI
Sbjct: 1   MSELQQQQEQPQQQQQQQQQPAYVYPDTAATSAGSQVPPSHHSNGSFGAVFIVLAVIVVI 60

Query: 61  SALACFLGRLCNRRVENGQRPKPEKTHSGARP-RGKEPKLHPDKEGDIEFGFDLRNPNGN 120
           SALACFLGRLCNRRVEN QRPKPEK+H+GARP RGKEPKLHPDKEGDIEFGFDLRNP+G 
Sbjct: 61  SALACFLGRLCNRRVENHQRPKPEKSHAGARPTRGKEPKLHPDKEGDIEFGFDLRNPSG- 120

Query: 121 KGKFPSSG-----GNGGSKGKSNGNGN-GTNGPNGHGNSFKPFENGGETRSEIKHTDHHE 180
           KGKFP  G     GNGGSKGK NGNG  G NGPNGHGNSFKPFENGGETRSEIKHTDHHE
Sbjct: 121 KGKFPPGGNGNGNGNGGSKGKGNGNGPIGLNGPNGHGNSFKPFENGGETRSEIKHTDHHE 180

Query: 181 GDFNFKAAGHH 183
           GDFNFKA GHH
Sbjct: 181 GDFNFKAGGHH 190

BLAST of Bhi07G001386 vs. ExPASy TrEMBL
Match: A0A1S3BF63 (hybrid signal transduction histidine kinase L OS=Cucumis melo OX=3656 GN=LOC103489006 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 2.2e-73
Identity = 165/191 (86.39%), Postives = 171/191 (89.53%), Query Frame = 0

Query: 1   MSELQQQQQQ-QPQQQQQQQPAFVYPNTAAT-VGSQPPPSHHSNGSFGAVFIVLAVIVVI 60
           MSELQQQQ+Q Q QQQQQQQPA+VYP+TAAT  GSQ PPSHHSNGSFGAVFIVLAVIVVI
Sbjct: 1   MSELQQQQEQPQQQQQQQQQPAYVYPDTAATSAGSQVPPSHHSNGSFGAVFIVLAVIVVI 60

Query: 61  SALACFLGRLCNRRVENGQRPKPEKTHSGARP-RGKEPKLHPDKEGDIEFGFDLRNPNGN 120
           SALACFLGRLCNRRVEN QRPKPEK+H+GARP RGKEPKLHPDKEGDIEFGFDLRNP+G 
Sbjct: 61  SALACFLGRLCNRRVENHQRPKPEKSHAGARPTRGKEPKLHPDKEGDIEFGFDLRNPSG- 120

Query: 121 KGKFPSSG-----GNGGSKGKSNGNGN-GTNGPNGHGNSFKPFENGGETRSEIKHTDHHE 180
           KGKFP  G     GNGGSKGK NGNG  G NGPNGHGNSFKPFENGGETRSEIKHTDHHE
Sbjct: 121 KGKFPPGGNGNGNGNGGSKGKGNGNGPIGLNGPNGHGNSFKPFENGGETRSEIKHTDHHE 180

Query: 181 GDFNFKAAGHH 183
           GDFNFKA GHH
Sbjct: 181 GDFNFKAGGHH 190

BLAST of Bhi07G001386 vs. ExPASy TrEMBL
Match: A0A0A0KTP5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G601520 PE=4 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 4.8e-73
Identity = 165/191 (86.39%), Postives = 170/191 (89.01%), Query Frame = 0

Query: 1   MSELQQQQQQQPQQQQQQQPAFVYPNTAAT-VGSQPPPSHHSNGSFGAVFIVLAVIVVIS 60
           MSELQQQQ Q  +QQQQQQPA+VYP+TAAT  GSQ PP HHSNGSFGAVFIVLAVIVVIS
Sbjct: 1   MSELQQQQPQ--RQQQQQQPAYVYPDTAATSAGSQVPPPHHSNGSFGAVFIVLAVIVVIS 60

Query: 61  ALACFLGRLCNRRVENGQRPKPEKTHSGARP-RGKEPKLHPDKEGDIEFGFDLRNPNGNK 120
           ALACFLGRLCNRRVEN QRPKPEK+HSGARP RGKEPKLHPDKEGDIEFGFDLRNP+G K
Sbjct: 61  ALACFLGRLCNRRVENHQRPKPEKSHSGARPTRGKEPKLHPDKEGDIEFGFDLRNPSG-K 120

Query: 121 GKFPSSG------GNGGSKGKSNGNGN-GTNGPNGHGNSFKPFENGGETRSEIKHTDHHE 180
           GKFPS G      GNGGSKGK NGNG  G NGPNGHGNSFKPFENGGETRSEIKHTDHHE
Sbjct: 121 GKFPSGGNGSTGNGNGGSKGKGNGNGPIGLNGPNGHGNSFKPFENGGETRSEIKHTDHHE 180

Query: 181 GDFNFKAAGHH 183
           GDFNFKAAGHH
Sbjct: 181 GDFNFKAAGHH 188

BLAST of Bhi07G001386 vs. ExPASy TrEMBL
Match: A0A6J1GWS9 (uncharacterized protein LOC111458233 OS=Cucurbita moschata OX=3662 GN=LOC111458233 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 4.2e-61
Identity = 139/172 (80.81%), Postives = 145/172 (84.30%), Query Frame = 0

Query: 14  QQQQQQPAFVYPNTAATVGSQPPPSHHSNGSFGAVFIVLAVIVVISALACFLGRLCNRRV 73
           QQQQQQPA VYPNTAA VGSQPPPSHHSNGSFGAVFIVLAVI+VIS +AC LGRLCNRR 
Sbjct: 5   QQQQQQPALVYPNTAA-VGSQPPPSHHSNGSFGAVFIVLAVILVISTVACLLGRLCNRRA 64

Query: 74  ENGQRPKPEKTHSGARPRGKEPKLHPDKEGDIEFGFDLRNPNGNKGKFPSSGGNGGSKGK 133
           EN QR KPEKTH GARPRGKEPKLHPDKEGDIEFG DLRNPNG KGK P SGGNGG+K  
Sbjct: 65  EN-QRAKPEKTHGGARPRGKEPKLHPDKEGDIEFGLDLRNPNG-KGKAP-SGGNGGTK-- 124

Query: 134 SNGNGNGTNGPNGHGNSFKPFENGGETRSEIKHT--DHHEGDFNFKAAGHHV 184
             GNG+G NGPNGH + FKPFENGGE R +IKH   DHHEGDF FKAA H V
Sbjct: 125 --GNGHGPNGPNGHSHGFKPFENGGEMRGQIKHADDDHHEGDFKFKAAAHPV 168

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G23530.11.2e-1237.71unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G70470.12.6e-1040.00unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G74055.11.6e-0438.10unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7ST812.2e-7386.39Hybrid signal transduction histidine kinase L OS=Cucumis melo var. makuwa OX=119... [more]
E5GBJ12.2e-7386.39Uncharacterized protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S3BF632.2e-7386.39hybrid signal transduction histidine kinase L OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A0A0KTP54.8e-7386.39Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G601520 PE=4 SV=1[more]
A0A6J1GWS94.2e-6180.81uncharacterized protein LOC111458233 OS=Cucurbita moschata OX=3662 GN=LOC1114582... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..40
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 75..183
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 75..108
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 117..149
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 158..183
NoneNo IPR availablePANTHERPTHR33429OS02G0708000 PROTEIN-RELATEDcoord: 2..172
NoneNo IPR availablePANTHERPTHR33429:SF7OS02G0708000 PROTEINcoord: 2..172
NoneNo IPR availableSUPERFAMILY81995beta-sandwich domain of Sec23/24coord: 5..42

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi07M001386Bhi07M001386mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016301 kinase activity