Bhi07G001275 (gene) Wax gourd (B227) v1

Overview


Name	Bhi07G001275
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Ribonuclease T(2)
Location	chr7: 44724959 .. 44726837 (-)
RNA-Seq Expression	Bhi07G001275
Synteny	Bhi07G001275

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCATCTTTTTGGCCTATAAAAAGTTGGATATTATTATCATTCCTTGAAACTCAAACAAAACAAACATGAAACCCAACTACTCAATCTTCATATTCAAGCTTCTCCTCTTGCAATATCTATCATCCCAGCTTTGTCTTTCTCAGGATTTCGATTTCTTCTACTTTGTTCAGCAGGTCAGTTAAATTAAATTCTATCTTTATAATTATTTTTCTTCTGTGAATTAATGAAAAAAATAATTTAATTAATTACTATGTATTTTTGTAGTGGCCGGGAGCATATTGCGATACAAAGCAACAACGGTGTTGCTATCCTAAAACAGGAAGGCCTGCTGCAGATTTTGGTATTCATGGCCTTTGGCCTAATTACAAAGATGGAAGCTACCCGTCCAACTGCGATCACAATAGCGTTTTCGACCGAACCCAGGTAATGTAATCGAATTAATTAGCACAAGATCCAACGAGTGAGTATATAGTATGATTATGGACTATATAGTGATAATTTATCCATGTTATTAGATTTCAGATGTATTAAGCAGCATGGAAAAGCATTGGCCATCATTGAGTTGTCCAAGCAGCAATGGGTTAAGATTTTGGTCACACGAATGGGAGAAGCATGGCACGTGCTCTGAGTCCGAACTAGACCAGAGAGAATATTTTGAAGCAGCTGTTAAACTCAAGGAGAAGGCCAATCTCCTCAAACTCCTTAATAATGCGGGTACGATTTGATGATTAAATTTACCATAGCTTTCGAGTCAATTGTGTTTAGTGATTTAAATAGTATTTTAGAGTTTTTTTTTTCTCATTTCCTCTCATTAAAAATTTGGATGGAGGCAGCCCAGACAGTACCCAATCAACATCTACCACATAACAATTTTTGTTTTAAAAATTTGATTTTTTTTTTCTTTCCACTATGTATGAAAAATGGCTGCCCTTAGCTTTATAGTCATCCATTAGTTTTTAAGTTTTTAAGGTGGCAAAGAATAGAAATTCTCTTAAAGGAATGACAAAATCATAAAAATATGTTGGCGTGGGATTCTGTTCAAGATCATTCCAAAACAAAATCTCAATATCGGTGTGATGCTTGTCCCAACAGACTCTGATATTTAAGTTGGTATCGAATCGAGTAAGGGATCTTGCATACCTCTTTGTGGTTTGGGGTGTTTCCTTATATATAACCTTGATCGTTCTTGACAAATTTGCCCAAATATTTGTATATGGACTTTGATTGTGTCAATTGGGAGGTCAGTCGTCGGATCTCATTTGGGGGTTGAGTCGTCTCTTACCTCGACTATCAAGTCCTAGAACAACTCTTTTTCCCTTTTGACACTTGAACCTGTCAAATCTCGTGGGTTGCAACGAATGGAATTTGTACCTACCTCAACTATTACAAAACATATTTTAATTTTTTGTTCACATGAAAACTCTACAAATTTTAACATAACATTTTGACTTAACCATGTCAATTAAATTGCAGGAATTGAAGCAAATGATGAGTTTTACAGTTTGGAGAGCGTAAGAAATGCCATAGAAGAAGGGATTGGGTTTACTCCTGGGATTCAATGTAATAAAAATTCAGCTGGCAATGCTCAACTTTACCAAATTTATTTATGTGTTGATACTTCTGGTTCTCAGTTCATCAAATGTCCAATTCTTCCCAAGCCAAAATGTCCTTCCACTATCCAATTCCCCAAGTTTTAATTTAATCGCTTTAATTCATTTCTAGCTACATTAATTTTACATTTTTTTTTAATTCCCTTGTAATTTCATTCGCTAATTATCCCTTATGTTTAGCTTTATGGATTATAACCAGCAATCATTTGCTTGCTAATAAAATTAATCACTTTGATTCATGTTTCTTCCCCCTTCTCCTAAACTATTTC

mRNA sequence

CCATCTTTTTGGCCTATAAAAAGTTGGATATTATTATCATTCCTTGAAACTCAAACAAAACAAACATGAAACCCAACTACTCAATCTTCATATTCAAGCTTCTCCTCTTGCAATATCTATCATCCCAGCTTTGTCTTTCTCAGGATTTCGATTTCTTCTACTTTGTTCAGCAGTGGCCGGGAGCATATTGCGATACAAAGCAACAACGGTGTTGCTATCCTAAAACAGGAAGGCCTGCTGCAGATTTTGGTATTCATGGCCTTTGGCCTAATTACAAAGATGGAAGCTACCCGTCCAACTGCGATCACAATAGCGTTTTCGACCGAACCCAGATTTCAGATGTATTAAGCAGCATGGAAAAGCATTGGCCATCATTGAGTTGTCCAAGCAGCAATGGGTTAAGATTTTGGTCACACGAATGGGAGAAGCATGGCACGTGCTCTGAGTCCGAACTAGACCAGAGAGAATATTTTGAAGCAGCTGTTAAACTCAAGGAGAAGGCCAATCTCCTCAAACTCCTTAATAATGCGGGAATTGAAGCAAATGATGAGTTTTACAGTTTGGAGAGCGTAAGAAATGCCATAGAAGAAGGGATTGGGTTTACTCCTGGGATTCAATGTAATAAAAATTCAGCTGGCAATGCTCAACTTTACCAAATTTATTTATGTGTTGATACTTCTGGTTCTCAGTTCATCAAATGTCCAATTCTTCCCAAGCCAAAATGTCCTTCCACTATCCAATTCCCCAAGTTTTAATTTAATCGCTTTAATTCATTTCTAGCTACATTAATTTTACATTTTTTTTTAATTCCCTTGTAATTTCATTCGCTAATTATCCCTTATGTTTAGCTTTATGGATTATAACCAGCAATCATTTGCTTGCTAATAAAATTAATCACTTTGATTCATGTTTCTTCCCCCTTCTCCTAAACTATTTC

Coding sequence (CDS)

ATGAAACCCAACTACTCAATCTTCATATTCAAGCTTCTCCTCTTGCAATATCTATCATCCCAGCTTTGTCTTTCTCAGGATTTCGATTTCTTCTACTTTGTTCAGCAGTGGCCGGGAGCATATTGCGATACAAAGCAACAACGGTGTTGCTATCCTAAAACAGGAAGGCCTGCTGCAGATTTTGGTATTCATGGCCTTTGGCCTAATTACAAAGATGGAAGCTACCCGTCCAACTGCGATCACAATAGCGTTTTCGACCGAACCCAGATTTCAGATGTATTAAGCAGCATGGAAAAGCATTGGCCATCATTGAGTTGTCCAAGCAGCAATGGGTTAAGATTTTGGTCACACGAATGGGAGAAGCATGGCACGTGCTCTGAGTCCGAACTAGACCAGAGAGAATATTTTGAAGCAGCTGTTAAACTCAAGGAGAAGGCCAATCTCCTCAAACTCCTTAATAATGCGGGAATTGAAGCAAATGATGAGTTTTACAGTTTGGAGAGCGTAAGAAATGCCATAGAAGAAGGGATTGGGTTTACTCCTGGGATTCAATGTAATAAAAATTCAGCTGGCAATGCTCAACTTTACCAAATTTATTTATGTGTTGATACTTCTGGTTCTCAGTTCATCAAATGTCCAATTCTTCCCAAGCCAAAATGTCCTTCCACTATCCAATTCCCCAAGTTTTAA

Protein sequence

MKPNYSIFIFKLLLLQYLSSQLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGIHGLWPNYKDGSYPSNCDHNSVFDRTQISDVLSSMEKHWPSLSCPSSNGLRFWSHEWEKHGTCSESELDQREYFEAAVKLKEKANLLKLLNNAGIEANDEFYSLESVRNAIEEGIGFTPGIQCNKNSAGNAQLYQIYLCVDTSGSQFIKCPILPKPKCPSTIQFPKF

Homology

[Close]

BLAST of Bhi07G001275 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 322.8 bits (826), Expect = 2.3e-88
Identity = 145/221 (65.61%), Postives = 182/221 (82.35%), Query Frame = 0


Query: 9   IFKLLLLQYLSSQLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGIHGLWP 68
           + KLL+ Q L       +DFDFFYFV QWPGAYCDT  + CCYP +G+PAADFGIHGLWP
Sbjct: 10  LLKLLVFQGLFVSR-PQEDFDFFYFVLQWPGAYCDT-SRACCYPTSGKPAADFGIHGLWP 69

Query: 69  NYKDGSYPSNCDHNSVFDRTQISDVLSSMEKHWPSLSCPSSNGLRFWSHEWEKHGTCSES 128
           NY  GS+PSNCD +S FDR+QISD++SS++K+WP+LSCPS+ G  FW HEWEKHGTCSES
Sbjct: 70  NYNGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSES 129

Query: 129 ELDQREYFEAAVKLKEKANLLKLLNNAGIEANDEFYSLESVRNAIEEGIGFTPGIQCNKN 188
            +DQ EYFE A+KLK+KANLL++L N+GI  +D FY+L+ + NAI++GIGFTPGI+CNK+
Sbjct: 130 VMDQHEYFENALKLKQKANLLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNKD 189

Query: 189 SAGNAQLYQIYLCVDTSGSQFIKCPILPKPKCPSTIQFPKF 230
              NAQL+QIY+CVDTSG++FI+CP+LP+  CPS IQF KF
Sbjct: 190 PERNAQLHQIYICVDTSGTEFIECPVLPRGSCPSQIQFSKF 228

[Close]

BLAST of Bhi07G001275 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 314.7 bits (805), Expect = 6.1e-86
Identity = 138/222 (62.16%), Postives = 182/222 (81.98%), Query Frame = 0


Query: 8   FIFKLLLLQYLSSQLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGIHGLW 67
           FIF +L LQ L  Q   +QDFDFFYFV QWPGAYCD++   CCYP+TG+PAADFGIHGLW
Sbjct: 4   FIF-ILALQQLYVQ-SFAQDFDFFYFVLQWPGAYCDSRHS-CCYPQTGKPAADFGIHGLW 63

Query: 68  PNYKDGSYPSNCDHNSVFDRTQISDVLSSMEKHWPSLSCPSSNGLRFWSHEWEKHGTCSE 127
           PNYK G +P NC+ +S FD  ++SD++S +++ WP+LSCPS++G++FW+HEWEKHGTC+E
Sbjct: 64  PNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAE 123

Query: 128 SELDQREYFEAAVKLKEKANLLKLLNNAGIEANDEFYSLESVRNAIEEGIGFTPGIQCNK 187
           SELDQ +YFEA +KLK+KANLL  L NAGI+ +D+FY ++ + N I++ +GF PGI+CNK
Sbjct: 124 SELDQHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNK 183

Query: 188 NSAGNAQLYQIYLCVDTSGSQFIKCPILPKPKCPSTIQFPKF 230
           +S+ N+QLYQIYLCVDTS S+FI CP++P  +C S +QFPKF
Sbjct: 184 DSSHNSQLYQIYLCVDTSASKFINCPVMPHGRCDSRVQFPKF 222

[Close]

BLAST of Bhi07G001275 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 308.5 bits (789), Expect = 4.4e-84
Identity = 135/218 (61.93%), Postives = 174/218 (79.82%), Query Frame = 0


Query: 12  LLLLQYLSSQLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGIHGLWPNYK 71
           L++L  + S    S+DFDFFYFVQQWPG+YCDT Q++CCYP +G+PAADFGIHGLWPNYK
Sbjct: 14  LVILPSVFSASSSSEDFDFFYFVQQWPGSYCDT-QKKCCYPNSGKPAADFGIHGLWPNYK 73

Query: 72  DGSYPSNCDHNSVFDRTQISDVLSSMEKHWPSLSCPSSNGLRFWSHEWEKHGTCSESELD 131
           DG+YPSNCD +  FD + ISD+L+SM+K WP+L+CPS +G  FW HEWEKHGTCSES +D
Sbjct: 74  DGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVID 133

Query: 132 QREYFEAAVKLKEKANLLKLLNNAGIEANDEFYSLESVRNAIEEGIGFTPGIQCNKNSAG 191
           Q EYF+ A+ LK+K NLL  L  AGI  + + YSLES+R++I+E IGFTP ++CN++ +G
Sbjct: 134 QHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSG 193

Query: 192 NAQLYQIYLCVDTSGSQFIKCPILPKPKCPSTIQFPKF 230
           N+QLYQ+YLCVD SGS  I+CP+ P  KC + I+FP F
Sbjct: 194 NSQLYQVYLCVDRSGSGLIECPVFPHGKCGAEIEFPSF 230

[Close]

BLAST of Bhi07G001275 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 226.5 bits (576), Expect = 2.2e-59
Identity = 110/227 (48.46%), Postives = 148/227 (65.20%), Query Frame = 0


Query: 7   IFIFKLLLLQYL---SSQLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGI 66
           I I  LL+LQ L   SSQ     DF+FFY+V  WPGA CD+ Q+ CC P  G  A+DF I
Sbjct: 4   IIIVSLLILQSLVVSSSQ--TEPDFNFFYWVNYWPGAICDS-QKGCCPPTKGNTASDFII 63

Query: 67  HGLWPNYKDGSYPSNCDHNSVFDRTQISDVLSSMEKHWPS---LSCPSSNGLRFWSHEWE 126
           HGLWP + +G++P+ CD  ++FD ++ISD++  MEK W      +CP SN    W HEW 
Sbjct: 64  HGLWPQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACP-SNETNLWEHEWN 123

Query: 127 KHGTCSESELDQREYFEAAVKLKEKANLLKLLNNAGIEANDEFYSLESVRNAIEEGIGFT 186
           KHGTC +S  DQ  YF   +K K K +LL +L   GI+ ND FYSL+ ++NAI+  IGF 
Sbjct: 124 KHGTCVQSIFDQHSYFRTNLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFA 183

Query: 187 PGIQCNKNSAGNAQLYQIYLCVDTSGSQFIKCPILPKPKCPSTIQFP 228
           PGI+CN++  GN QL+QIY+C+D    +F++CP +P   C S I+FP
Sbjct: 184 PGIECNEDVKGNKQLFQIYICLDNYAKEFVECPYVPDKSCASKIKFP 226

[Close]

BLAST of Bhi07G001275 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 135.6 bits (340), Expect = 5.1e-32
Identity = 81/217 (37.33%), Postives = 116/217 (53.46%), Query Frame = 0


Query: 26  QDFDFFYFVQQWPGAYCDTKQQRC----CYPKTGRPAADFGIHGLWPNYKDGSYPSNCDH 85
           ++FD+F    QWPG YC   +  C    C   +  P   F IHGLWP+Y DGS+PS C +
Sbjct: 30  REFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAP-TQFTIHGLWPDYNDGSWPS-CCY 89

Query: 86  NSVFDRTQISDVLSSMEKHWPSLSC--PSS-NGLR--FWSHEWEKHGTCSESEL-DQREY 145
            S F   +IS ++  +EK+WPSLSC  PSS NG +  FW HEWEKHGTCS     D+  Y
Sbjct: 90  RSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNY 149

Query: 146 FEAAVKLKEKANLLKLLNNAG-IEANDEFYSLESVRNAIEEGIGFTPGIQCNKNSAGNAQ 205
           F   + L  K N+  +L  AG + +N E Y L  +  AI+     TP + C +++    +
Sbjct: 150 FLTTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRDAIDEIR 209

Query: 206 L--YQIYLCVDTSGSQFIKCPILPKPKCPSTIQFPKF 230
           +  Y+ +   D  GSQ     +  +  CP  +  P++
Sbjct: 210 ICFYKDFKPRDCVGSQ----DLTSRKSCPKYVSLPEY 240

[Close]

BLAST of Bhi07G001275 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 8.6e-85
Identity = 138/222 (62.16%), Postives = 182/222 (81.98%), Query Frame = 0


Query: 8   FIFKLLLLQYLSSQLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGIHGLW 67
           FIF +L LQ L  Q   +QDFDFFYFV QWPGAYCD++   CCYP+TG+PAADFGIHGLW
Sbjct: 4   FIF-ILALQQLYVQ-SFAQDFDFFYFVLQWPGAYCDSRHS-CCYPQTGKPAADFGIHGLW 63

Query: 68  PNYKDGSYPSNCDHNSVFDRTQISDVLSSMEKHWPSLSCPSSNGLRFWSHEWEKHGTCSE 127
           PNYK G +P NC+ +S FD  ++SD++S +++ WP+LSCPS++G++FW+HEWEKHGTC+E
Sbjct: 64  PNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAE 123

Query: 128 SELDQREYFEAAVKLKEKANLLKLLNNAGIEANDEFYSLESVRNAIEEGIGFTPGIQCNK 187
           SELDQ +YFEA +KLK+KANLL  L NAGI+ +D+FY ++ + N I++ +GF PGI+CNK
Sbjct: 124 SELDQHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNK 183

Query: 188 NSAGNAQLYQIYLCVDTSGSQFIKCPILPKPKCPSTIQFPKF 230
           +S+ N+QLYQIYLCVDTS S+FI CP++P  +C S +QFPKF
Sbjct: 184 DSSHNSQLYQIYLCVDTSASKFINCPVMPHGRCDSRVQFPKF 222

[Close]

BLAST of Bhi07G001275 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 6.2e-83
Identity = 135/218 (61.93%), Postives = 174/218 (79.82%), Query Frame = 0


Query: 12  LLLLQYLSSQLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGIHGLWPNYK 71
           L++L  + S    S+DFDFFYFVQQWPG+YCDT Q++CCYP +G+PAADFGIHGLWPNYK
Sbjct: 14  LVILPSVFSASSSSEDFDFFYFVQQWPGSYCDT-QKKCCYPNSGKPAADFGIHGLWPNYK 73

Query: 72  DGSYPSNCDHNSVFDRTQISDVLSSMEKHWPSLSCPSSNGLRFWSHEWEKHGTCSESELD 131
           DG+YPSNCD +  FD + ISD+L+SM+K WP+L+CPS +G  FW HEWEKHGTCSES +D
Sbjct: 74  DGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVID 133

Query: 132 QREYFEAAVKLKEKANLLKLLNNAGIEANDEFYSLESVRNAIEEGIGFTPGIQCNKNSAG 191
           Q EYF+ A+ LK+K NLL  L  AGI  + + YSLES+R++I+E IGFTP ++CN++ +G
Sbjct: 134 QHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSG 193

Query: 192 NAQLYQIYLCVDTSGSQFIKCPILPKPKCPSTIQFPKF 230
           N+QLYQ+YLCVD SGS  I+CP+ P  KC + I+FP F
Sbjct: 194 NSQLYQVYLCVDRSGSGLIECPVFPHGKCGAEIEFPSF 230

[Close]

BLAST of Bhi07G001275 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 295.4 bits (755), Expect = 5.4e-79
Identity = 135/226 (59.73%), Postives = 177/226 (78.32%), Query Frame = 0


Query: 5   YSIFIFKLLLLQYLSSQLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGIH 64
           +S+F+  L++ Q L S L  ++DFDFFYFVQQWPG+YCDTKQ  CCYP TG+PAADFGIH
Sbjct: 7   FSLFLILLIITQCL-SVLNAAKDFDFFYFVQQWPGSYCDTKQS-CCYPTTGKPAADFGIH 66

Query: 65  GLWPNYKDGSYPSNCDHNSVFDRTQISDVLSSMEKHWPSLSCPSSNGLRFWSHEWEKHGT 124
           GLWPN  DG+YPSNCD NS +D++QISD++SSM+++WP+L+CPS +G  FWSHEWEKHGT
Sbjct: 67  GLWPNNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGT 126

Query: 125 CSESEL-DQREYFEAAVKLKEKANLLKLLNNAGIEANDEFYSLESVRNAIEEGIGFTPGI 184
           C+ES L +Q  YF+ A+ LK + +LL +L  A I  + E Y L ++RNAI+  IG+TP I
Sbjct: 127 CAESVLTNQHAYFKKALDLKNQIDLLSILQGADIHPDGESYDLVNIRNAIKSAIGYTPWI 186

Query: 185 QCNKNSAGNAQLYQIYLCVDTSGSQFIKCPILPKPKCPSTIQFPKF 230
           QCN + +GN+QLYQ+Y+CVD SGS  I+CPI P  KC ++I+FP F
Sbjct: 187 QCNVDQSGNSQLYQVYICVDGSGSSLIECPIFPGGKCGTSIEFPTF 230

[Close]

BLAST of Bhi07G001275 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 287.7 bits (735), Expect = 1.1e-76
Identity = 132/223 (59.19%), Postives = 170/223 (76.23%), Query Frame = 0


Query: 9   IFKLLLLQYLSSQLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAADFGIHGLWP 68
           + K++++Q L      SQDFDFFYFVQQWP +YCDT++  CCYP TG+P  DF IHGLWP
Sbjct: 9   LIKIIVVQCLLVLCVTSQDFDFFYFVQQWPASYCDTRRS-CCYPTTGKPDEDFSIHGLWP 68

Query: 69  NYKDGSYPSNCDHNSVFDRTQISDVLSSMEKHWPSLSCPSSNGLRFWSHEWEKHGTCSES 128
           NYKDG +P NCD  S  D ++ SD++S+MEK+WPSL+CPSS+GL+FWSHEW KHGTC  S
Sbjct: 69  NYKDGKWPQNCDRESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWLKHGTC--S 128

Query: 129 ELDQREYFEAAVKLKEKANLLKLLNNAGIE-ANDEFYSLESVRNAIEEGIGFTPGIQCNK 188
            L+Q  YF+ A+  K K+NLL+ LNNAGI+  N ++Y +ES++ AIE+G+G TP I+CN 
Sbjct: 129 ALNQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIEKGVGHTPFIECNV 188

Query: 189 NSAGNAQLYQIYLCVDTSGSQFIKCPILPK-PKCPSTIQFPKF 230
           +S GN QLYQ+YLCVD+S S+FI CPI P   KC S I+FP F
Sbjct: 189 DSQGNHQLYQVYLCVDSSASKFIDCPIFPHGGKCGSKIEFPSF 228

[Close]

BLAST of Bhi07G001275 vs. ExPASy Swiss-Prot
Match: P42814 (Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 7.2e-31
Identity = 81/217 (37.33%), Postives = 116/217 (53.46%), Query Frame = 0


Query: 26  QDFDFFYFVQQWPGAYCDTKQQRC----CYPKTGRPAADFGIHGLWPNYKDGSYPSNCDH 85
           ++FD+F    QWPG YC   +  C    C   +  P   F IHGLWP+Y DGS+PS C +
Sbjct: 30  REFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAP-TQFTIHGLWPDYNDGSWPS-CCY 89

Query: 86  NSVFDRTQISDVLSSMEKHWPSLSC--PSS-NGLR--FWSHEWEKHGTCSESEL-DQREY 145
            S F   +IS ++  +EK+WPSLSC  PSS NG +  FW HEWEKHGTCS     D+  Y
Sbjct: 90  RSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNY 149

Query: 146 FEAAVKLKEKANLLKLLNNAG-IEANDEFYSLESVRNAIEEGIGFTPGIQCNKNSAGNAQ 205
           F   + L  K N+  +L  AG + +N E Y L  +  AI+     TP + C +++    +
Sbjct: 150 FLTTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRDAIDEIR 209

Query: 206 L--YQIYLCVDTSGSQFIKCPILPKPKCPSTIQFPKF 230
           +  Y+ +   D  GSQ     +  +  CP  +  P++
Sbjct: 210 ICFYKDFKPRDCVGSQ----DLTSRKSCPKYVSLPEY 240

[Close]

BLAST of Bhi07G001275 vs. ExPASy TrEMBL
Match: A0A0A0KVY9 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_5G605150 PE=3 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 7.3e-119
Identity = 202/229 (88.21%), Postives = 221/229 (96.51%), Query Frame = 0


Query: 1   MKPNYSIFIFKLLLLQYLSSQLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAAD 60
           MK NYSIFIF LL+LQ+LS+ LCLSQDFDFFYFVQQWPGAYCDTK+Q CCYP+TGRPAAD
Sbjct: 1   MKFNYSIFIFNLLILQHLST-LCLSQDFDFFYFVQQWPGAYCDTKRQHCCYPETGRPAAD 60

Query: 61  FGIHGLWPNYKDGSYPSNCDHNSVFDRTQISDVLSSMEKHWPSLSCPSSNGLRFWSHEWE 120
           FGIHGLWPNYKDGSYPSNCD +SVFDRTQIS+VLSSM+KHWPSLSCPSSNGLRFWSHEWE
Sbjct: 61  FGIHGLWPNYKDGSYPSNCDPDSVFDRTQISEVLSSMDKHWPSLSCPSSNGLRFWSHEWE 120

Query: 121 KHGTCSESELDQREYFEAAVKLKEKANLLKLLNNAGIEANDEFYSLESVRNAIEEGIGFT 180
           KHGTCSESELDQ+EYFEAA+KLKEKANLLK+LN+AGIEANDE YSLESV+NAIEEGIGFT
Sbjct: 121 KHGTCSESELDQKEYFEAAIKLKEKANLLKVLNSAGIEANDEMYSLESVKNAIEEGIGFT 180

Query: 181 PGIQCNKNSAGNAQLYQIYLCVDTSGSQFIKCPILPKPKCPSTIQFPKF 230
           PGI+CN++SAGNAQLYQ+YLCVDTSGS+FIKCPILP+ KC STIQFPKF
Sbjct: 181 PGIECNRDSAGNAQLYQVYLCVDTSGSEFIKCPILPRTKCASTIQFPKF 228

[Close]

BLAST of Bhi07G001275 vs. ExPASy TrEMBL
Match: A0A5A7SY93 (Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G004270 PE=3 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 9.5e-119
Identity = 204/229 (89.08%), Postives = 219/229 (95.63%), Query Frame = 0


Query: 1   MKPNYSIFIFKLLLLQYLSSQLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAAD 60
           MK NYSIFIF LLLLQ LSS LCLSQDFDFFYFVQQWPGAYCDTK+Q CCYPKTGRP AD
Sbjct: 1   MKFNYSIFIFNLLLLQPLSS-LCLSQDFDFFYFVQQWPGAYCDTKKQHCCYPKTGRPVAD 60

Query: 61  FGIHGLWPNYKDGSYPSNCDHNSVFDRTQISDVLSSMEKHWPSLSCPSSNGLRFWSHEWE 120
           FGIHGLWPNYKDG+YPS+C+ +SVFDRTQIS+VLSSMEKHWPSLSCPSSNGLRFWSHEWE
Sbjct: 61  FGIHGLWPNYKDGTYPSDCNPDSVFDRTQISEVLSSMEKHWPSLSCPSSNGLRFWSHEWE 120

Query: 121 KHGTCSESELDQREYFEAAVKLKEKANLLKLLNNAGIEANDEFYSLESVRNAIEEGIGFT 180
           KHGTCSESELDQ+EYFEAA+KLKEKANLL +LNNAGIEANDE YSLESV+NAIEEGIGFT
Sbjct: 121 KHGTCSESELDQKEYFEAAIKLKEKANLLNVLNNAGIEANDEMYSLESVKNAIEEGIGFT 180

Query: 181 PGIQCNKNSAGNAQLYQIYLCVDTSGSQFIKCPILPKPKCPSTIQFPKF 230
           PGI+CNK+SAGN+QLYQIYLCVDTSGS+FIKCPILP+PKC STIQFPKF
Sbjct: 181 PGIECNKDSAGNSQLYQIYLCVDTSGSEFIKCPILPRPKCASTIQFPKF 228

[Close]

BLAST of Bhi07G001275 vs. ExPASy TrEMBL
Match: A0A1S3BEW0 (Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103489123 PE=3 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 9.5e-119
Identity = 204/229 (89.08%), Postives = 219/229 (95.63%), Query Frame = 0


Query: 1   MKPNYSIFIFKLLLLQYLSSQLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAAD 60
           MK NYSIFIF LLLLQ LSS LCLSQDFDFFYFVQQWPGAYCDTK+Q CCYPKTGRP AD
Sbjct: 1   MKFNYSIFIFNLLLLQPLSS-LCLSQDFDFFYFVQQWPGAYCDTKKQHCCYPKTGRPVAD 60

Query: 61  FGIHGLWPNYKDGSYPSNCDHNSVFDRTQISDVLSSMEKHWPSLSCPSSNGLRFWSHEWE 120
           FGIHGLWPNYKDG+YPS+C+ +SVFDRTQIS+VLSSMEKHWPSLSCPSSNGLRFWSHEWE
Sbjct: 61  FGIHGLWPNYKDGTYPSDCNPDSVFDRTQISEVLSSMEKHWPSLSCPSSNGLRFWSHEWE 120

Query: 121 KHGTCSESELDQREYFEAAVKLKEKANLLKLLNNAGIEANDEFYSLESVRNAIEEGIGFT 180
           KHGTCSESELDQ+EYFEAA+KLKEKANLL +LNNAGIEANDE YSLESV+NAIEEGIGFT
Sbjct: 121 KHGTCSESELDQKEYFEAAIKLKEKANLLNVLNNAGIEANDEMYSLESVKNAIEEGIGFT 180

Query: 181 PGIQCNKNSAGNAQLYQIYLCVDTSGSQFIKCPILPKPKCPSTIQFPKF 230
           PGI+CNK+SAGN+QLYQIYLCVDTSGS+FIKCPILP+PKC STIQFPKF
Sbjct: 181 PGIECNKDSAGNSQLYQIYLCVDTSGSEFIKCPILPRPKCASTIQFPKF 228

[Close]

BLAST of Bhi07G001275 vs. ExPASy TrEMBL
Match: A0A6J1H0Y9 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111458576 PE=3 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 4.1e-114
Identity = 191/229 (83.41%), Postives = 217/229 (94.76%), Query Frame = 0


Query: 1   MKPNYSIFIFKLLLLQYLSSQLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAAD 60
           MKP+YSIFIF LLLLQYL S LCL+QDFDFFYFVQQWPGAYC++KQQRCCYPKTGRP+AD
Sbjct: 1   MKPSYSIFIFNLLLLQYL-SPLCLAQDFDFFYFVQQWPGAYCNSKQQRCCYPKTGRPSAD 60

Query: 61  FGIHGLWPNYKDGSYPSNCDHNSVFDRTQISDVLSSMEKHWPSLSCPSSNGLRFWSHEWE 120
           FGIHGLWPNYKDGSYPSNC+ NS FDRTQIS+ LSSM+K WPSLSCPSS+GLRFWSHEWE
Sbjct: 61  FGIHGLWPNYKDGSYPSNCNSNSAFDRTQISEALSSMQKDWPSLSCPSSDGLRFWSHEWE 120

Query: 121 KHGTCSESELDQREYFEAAVKLKEKANLLKLLNNAGIEANDEFYSLESVRNAIEEGIGFT 180
           KHGTCSESELDQ+EYFEAA++LKEKANLLKLL +AGIEANDEFYSLES+R AI++G+G+T
Sbjct: 121 KHGTCSESELDQKEYFEAAIRLKEKANLLKLLYDAGIEANDEFYSLESIREAIQKGVGYT 180

Query: 181 PGIQCNKNSAGNAQLYQIYLCVDTSGSQFIKCPILPKPKCPSTIQFPKF 230
           PGIQCNK+S G++QLYQ+YLCVDTSGS+FIKCP+LP+ KC S+IQFPKF
Sbjct: 181 PGIQCNKDSGGHSQLYQVYLCVDTSGSEFIKCPVLPRGKCSSSIQFPKF 228

[Close]

BLAST of Bhi07G001275 vs. ExPASy TrEMBL
Match: A0A6J1K2J7 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111489512 PE=3 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 7.0e-114
Identity = 191/229 (83.41%), Postives = 214/229 (93.45%), Query Frame = 0


Query: 1   MKPNYSIFIFKLLLLQYLSSQLCLSQDFDFFYFVQQWPGAYCDTKQQRCCYPKTGRPAAD 60
           MKP+YSIFIF LLLLQYL S LCL+QDFDFFYFVQQWPGAYC++KQ RCCYPKTGRP+AD
Sbjct: 1   MKPSYSIFIFNLLLLQYL-SPLCLAQDFDFFYFVQQWPGAYCNSKQHRCCYPKTGRPSAD 60

Query: 61  FGIHGLWPNYKDGSYPSNCDHNSVFDRTQISDVLSSMEKHWPSLSCPSSNGLRFWSHEWE 120
           FGIHGLWPNYKDG YPSNC+ NS FDRTQIS+ LSSM+K WPSLSCPSS+GLRFWSHEWE
Sbjct: 61  FGIHGLWPNYKDGGYPSNCNSNSAFDRTQISEALSSMQKDWPSLSCPSSDGLRFWSHEWE 120

Query: 121 KHGTCSESELDQREYFEAAVKLKEKANLLKLLNNAGIEANDEFYSLESVRNAIEEGIGFT 180
           KHGTCSESELDQ+EYFEAA+KLKEKANLL LLN+AGIEANDEFYSLES+R AI +G+GFT
Sbjct: 121 KHGTCSESELDQKEYFEAAIKLKEKANLLNLLNDAGIEANDEFYSLESIREAIRKGVGFT 180

Query: 181 PGIQCNKNSAGNAQLYQIYLCVDTSGSQFIKCPILPKPKCPSTIQFPKF 230
           PGIQCNK+S G++QLYQ+YLCVDTSGS+FIKCP+LP+ KC S+IQFPKF
Sbjct: 181 PGIQCNKDSGGHSQLYQVYLCVDTSGSEFIKCPVLPRGKCSSSIQFPKF 228

The following BLAST results are available for this feature:

BLAST of Bhi07G001275 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14220.1	2.3e-88	65.61	Ribonuclease T2 family protein	[more]
AT1G26820.1	6.1e-86	62.16	ribonuclease 3	[more]
AT2G02990.1	4.4e-84	61.93	ribonuclease 1	[more]
AT1G14210.1	2.2e-59	48.46	Ribonuclease T2 family protein	[more]
AT2G39780.1	5.1e-32	37.33	ribonuclease 2	[more]

BLAST of Bhi07G001275 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P42815	8.6e-85	62.16	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
P42813	6.2e-83	61.93	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P80022	5.4e-79	59.73	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P80196	1.1e-76	59.19	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]
P42814	7.2e-31	37.33	Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1	[more]

BLAST of Bhi07G001275 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KVY9	7.3e-119	88.21	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_5G605150 PE=3 SV=1	[more]
A0A5A7SY93	9.5e-119	89.08	Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G004...	[more]
A0A1S3BEW0	9.5e-119	89.08	Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103489123 PE=3 SV=1	[more]
A0A6J1H0Y9	4.1e-114	83.41	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111458576 PE=3 SV=1	[more]
A0A6J1K2J7	7.0e-114	83.41	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111489512 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 28..212 e-value: 1.6E-57 score: 194.6
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 5..229
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 22..229 e-value: 1.9E-77 score: 262.0
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 23..228
None	No IPR available	PANTHER	PTHR11240:SF55	F7A19.32 PROTEIN	coord: 5..229
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 61..68
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 114..125
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 28..223 e-value: 1.01129E-77 score: 230.297

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi07M001275	Bhi07M001275	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006401	RNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding