Bhi07G000698 (gene) Wax gourd (B227) v1

Overview


Name	Bhi07G000698
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	protein NUCLEAR FUSION DEFECTIVE 4-like
Location	chr7: 28421469 .. 28421937 (-)
RNA-Seq Expression	Bhi07G000698
Synteny	Bhi07G000698

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGGCCGGCGGAGGAATCTTGAGGTGCTCCCACATAAGGAGCATCGCCGTCCACGTCATCACCGGACGGTGGTTTGTGGTGTTCGCTTCACTCCTAATCATGGCGACAACTGGAGCGACGTACATGTTCGGCCTATATTCCGATGACATAAAATCGGTTTTGGGTTACGACCAAACAACGTTGAATTTGTTGAGTTTTTTTAAGGATTTGGGTGCAAACGTCGGCGTTTTGTCTGGGCTTATTAACGAAGTTACGCCGCCGTGGGTGGTTCTATCCATCGGCGCCATTCTGAATTTCTTTGGTTACTTTATGATTTGGCTCGCCGTTACTCGACGGATCTCCGCCCCCAAAGTTTGGCAAATGTGTGTCTATATTTGTATTGGAGCCAATTCTCAGTCCTTTGCTAATACGGGTTCTCTTGTTACTTGTGTCAAGAACTTTCCTGAGAGTCGCGGCGTTGTTCTCA

mRNA sequence

ATGGTGGCCGGCGGAGGAATCTTGAGGTGCTCCCACATAAGGAGCATCGCCGTCCACGTCATCACCGGACGGTGGTTTGTGGTGTTCGCTTCACTCCTAATCATGGCGACAACTGGAGCGACGTACATGTTCGGCCTATATTCCGATGACATAAAATCGGTTTTGGGTTACGACCAAACAACGTTGAATTTGTTGAGTTTTTTTAAGGATTTGGGTGCAAACGTCGGCGTTTTGTCTGGGCTTATTAACGAAGTTACGCCGCCGTGGGTGGTTCTATCCATCGGCGCCATTCTGAATTTCTTTGGTTACTTTATGATTTGGCTCGCCGTTACTCGACGGATCTCCGCCCCCAAAGTTTGGCAAATGTGTGTCTATATTTGTATTGGAGCCAATTCTCAGTCCTTTGCTAATACGGGTTCTCTTGTTACTTGTGTCAAGAACTTTCCTGAGAGTCGCGGCGTTGTTCTCA

Coding sequence (CDS)

ATGGTGGCCGGCGGAGGAATCTTGAGGTGCTCCCACATAAGGAGCATCGCCGTCCACGTCATCACCGGACGGTGGTTTGTGGTGTTCGCTTCACTCCTAATCATGGCGACAACTGGAGCGACGTACATGTTCGGCCTATATTCCGATGACATAAAATCGGTTTTGGGTTACGACCAAACAACGTTGAATTTGTTGAGTTTTTTTAAGGATTTGGGTGCAAACGTCGGCGTTTTGTCTGGGCTTATTAACGAAGTTACGCCGCCGTGGGTGGTTCTATCCATCGGCGCCATTCTGAATTTCTTTGGTTACTTTATGATTTGGCTCGCCGTTACTCGACGGATCTCCGCCCCCAAAGTTTGGCAAATGTGTGTCTATATTTGTATTGGAGCCAATTCTCAGTCCTTTGCTAATACGGGTTCTCTTGTTACTTGTGTCAAGAACTTTCCTGAGAGTCGCGGCGTTGTTCTCA

Protein sequence

MVAGGGILRCSHIRSIAVHVITGRWFVVFASLLIMATTGATYMFGLYSDDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAILNFFGYFMIWLAVTRRISAPKVWQMCVYICIGANSQSFANTGSLVTCVKNFPESRGVVL

Homology

[Close]

BLAST of Bhi07G000698 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 248.4 bits (633), Expect = 3.7e-66
Identity = 116/144 (80.56%), Postives = 131/144 (90.97%), Query Frame = 0


Query: 13  IRSIAVHVITGRWFVVFASLLIMATTGATYMFGLYSDDIKSVLGYDQTTLNLLSFFKDLG 72
           ++S+ + ++TGRWF+ F SLLIM+T GATYMFG+YS DIK  LGYDQTTLNLLSFFKDLG
Sbjct: 10  MKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLG 69

Query: 73  ANVGVLSGLINEVTPPWVVLSIGAILNFFGYFMIWLAVTRRISAPKVWQMCVYICIGANS 132
           ANVGVL+GL+NEVTPPW +L IGAILNFFGYFMIWLAVT RIS P+VW MC+YIC+GANS
Sbjct: 70  ANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANS 129

Query: 133 QSFANTGSLVTCVKNFPESRGVVL 157
           QSFANTGSLVTCVKNFPESRGVVL
Sbjct: 130 QSFANTGSLVTCVKNFPESRGVVL 153

[Close]

BLAST of Bhi07G000698 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 228.8 bits (582), Expect = 3.0e-60
Identity = 108/139 (77.70%), Postives = 121/139 (87.05%), Query Frame = 0


Query: 18  VHVITGRWFVVFASLLIMATTGATYMFGLYSDDIKSVLGYDQTTLNLLSFFKDLGANVGV 77
           +H   GRWF+VFAS LIMA  GATY+FG YS DIKS LGYDQTTLNLL FFKDLGANVGV
Sbjct: 11  IHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVGV 70

Query: 78  LSGLINEVTPPWVVLSIGAILNFFGYFMIWLAVTRRISAPKVWQMCVYICIGANSQSFAN 137
           LSGLI EVTP W VL+IG+ +NF GYFMIWL VT +++ PKVWQMC+YICIGANSQ+FAN
Sbjct: 71  LSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFAN 130

Query: 138 TGSLVTCVKNFPESRGVVL 157
           TG+LVTCVKNFPESRGV+L
Sbjct: 131 TGALVTCVKNFPESRGVML 149

[Close]

BLAST of Bhi07G000698 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 122.1 bits (305), Expect = 4.0e-28
Identity = 63/146 (43.15%), Postives = 88/146 (60.27%), Query Frame = 0


Query: 11  SHIRSIAVHVITGRWFVVFASLLIMATTGATYMFGLYSDDIKSVLGYDQTTLNLLSFFKD 70
           S  R   V  I  RW V  A++ I +  G  Y+FG  S  IKS L Y+Q  L+ L   KD
Sbjct: 3   STTREKFVSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKD 62

Query: 71  LGANVGVLSGLINEVTPPWVVLSIGAILNFFGYFMIWLAVTRRISAPKVWQMCVYICIGA 130
           LG +VG ++G ++E+ P W  L +GA+ N  GY  +WL VT R     +W MCV I +G 
Sbjct: 63  LGDSVGFIAGTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGN 122

Query: 131 NSQSFANTGSLVTCVKNFPESRGVVL 157
           N +++ NTG+LV+ V+NFP+SRG V+
Sbjct: 123 NGETYFNTGALVSGVQNFPKSRGPVV 148

[Close]

BLAST of Bhi07G000698 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 121.7 bits (304), Expect = 5.2e-28
Identity = 63/155 (40.65%), Postives = 93/155 (60.00%), Query Frame = 0


Query: 16  IAVHVITGRWFVVFASLLIMATTGATYMFGLYSDDIKSVLGYDQTTLNLLSFFKDLGANV 75
           +A  ++  +W  + AS+ I  + G +Y FG+YS  +KS   YDQ+TL+ +S FKD+G NV
Sbjct: 1   MATEILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNV 60

Query: 76  GVLSGLINEVTP--------------PWVVLSIGAILNFFGYFMIWLAVTRRISAPKVWQ 135
           GVLSGL+                   PWVV+ IGAILNF GYF++W +VT  I  P V  
Sbjct: 61  GVLSGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPV 120

Query: 136 MCVYICIGANSQSFANTGSLVTCVKNFPESRGVVL 157
           MC+++ I A S +F NT ++V+ ++NF +  G  +
Sbjct: 121 MCLFMFIAAQSLTFLNTANVVSSLENFADYGGTAV 155

[Close]

BLAST of Bhi07G000698 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 119.4 bits (298), Expect = 2.6e-27
Identity = 58/138 (42.03%), Postives = 88/138 (63.77%), Query Frame = 0


Query: 20  VITGRWFVVFASLLIMATTGATYMF-GLYSDDIKSVLGYDQTTLNLLSFFKDLGANVGVL 79
           ++  RW V   ++ + +  G  Y+F G  S  IK+ LGY+Q  + LL   K+LG  +G +
Sbjct: 12  LVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFV 71

Query: 80  SGLINEVTPPWVVLSIGAILNFFGYFMIWLAVTRRISAPKVWQMCVYICIGANSQSFANT 139
           SG ++EV+P WVVL +GA  N FGY ++WL VT ++    +W + V I +G N +++ NT
Sbjct: 72  SGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNT 131

Query: 140 GSLVTCVKNFPESRGVVL 157
            SLV+C+ NFPESRG V+
Sbjct: 132 ASLVSCIHNFPESRGPVV 149

[Close]

BLAST of Bhi07G000698 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.1e-14
Identity = 48/135 (35.56%), Postives = 71/135 (52.59%), Query Frame = 0


Query: 24  RWFVVFASLLIMATTGATYMFGLYSDDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLIN 83
           +W V+ A++ I A+TG  + F  YS  +KSVLG  Q  LN L+   DLG   G  SG+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 84  EVTPPWVVLSIGAILNFFGYFMIWLAVTRRISAPKVWQMCVYIC--IGANSQSFANTGSL 143
              P  VVL   A + F GY + WL +T  I+ P      V++C  +   S  + NT   
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP---YSLVFLCCLLAGLSICWFNTACF 162

Query: 144 VTCVKNFPESRGVVL 157
           + C+++FP +R + L
Sbjct: 163 ILCIRHFPNNRALAL 174

[Close]

BLAST of Bhi07G000698 vs. ExPASy TrEMBL
Match: A0A5D3CFW3 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002400 PE=4 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 1.5e-75
Identity = 146/156 (93.59%), Postives = 148/156 (94.87%), Query Frame = 0


Query: 1   MVAGGGILRCSHIRSIAVHVITGRWFVVFASLLIMATTGATYMFGLYSDDIKSVLGYDQT 60
           MVAGGGI  CS I SIAVHVITGRWFVVFASLLIMA  GATYMFGLYS DIKSVLGYDQT
Sbjct: 1   MVAGGGISGCSDIGSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAILNFFGYFMIWLAVTRRISAPKVW 120
           TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGA+LNFFGYFMIWLAVTRRIS PKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVW 120

Query: 121 QMCVYICIGANSQSFANTGSLVTCVKNFPESRGVVL 157
           QMC+YICIGANSQSFANTGSLVTCVKNFPESRGVVL
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVL 156

[Close]

BLAST of Bhi07G000698 vs. ExPASy TrEMBL
Match: A0A1S3BG09 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 PE=4 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 1.5e-75
Identity = 146/156 (93.59%), Postives = 148/156 (94.87%), Query Frame = 0


Query: 1   MVAGGGILRCSHIRSIAVHVITGRWFVVFASLLIMATTGATYMFGLYSDDIKSVLGYDQT 60
           MVAGGGI  CS I SIAVHVITGRWFVVFASLLIMA  GATYMFGLYS DIKSVLGYDQT
Sbjct: 1   MVAGGGISGCSDIGSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAILNFFGYFMIWLAVTRRISAPKVW 120
           TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGA+LNFFGYFMIWLAVTRRIS PKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVW 120

Query: 121 QMCVYICIGANSQSFANTGSLVTCVKNFPESRGVVL 157
           QMC+YICIGANSQSFANTGSLVTCVKNFPESRGVVL
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVL 156

[Close]

BLAST of Bhi07G000698 vs. ExPASy TrEMBL
Match: A0A6J1GZI8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111458291 PE=4 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.7e-74
Identity = 143/156 (91.67%), Postives = 148/156 (94.87%), Query Frame = 0


Query: 1   MVAGGGILRCSHIRSIAVHVITGRWFVVFASLLIMATTGATYMFGLYSDDIKSVLGYDQT 60
           MVAGGGI  CS I SIAVHVITGRWFVVFASLLIMA  GATYMF LYS DIKSVLGYDQT
Sbjct: 1   MVAGGGISGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAILNFFGYFMIWLAVTRRISAPKVW 120
           TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGA++NFFGYFMIWLAVTR+I+APKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTRKIAAPKVW 120

Query: 121 QMCVYICIGANSQSFANTGSLVTCVKNFPESRGVVL 157
           QMC+YICIGANSQSFANTGSLVTCVKNFPESRGVVL
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVL 156

[Close]

BLAST of Bhi07G000698 vs. ExPASy TrEMBL
Match: A0A6J1K186 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489721 PE=4 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 4.9e-74
Identity = 143/156 (91.67%), Postives = 147/156 (94.23%), Query Frame = 0


Query: 1   MVAGGGILRCSHIRSIAVHVITGRWFVVFASLLIMATTGATYMFGLYSDDIKSVLGYDQT 60
           MVAGGGI  CS I SIAVHVITGRWFVVFASLLIMA  GATYMF LYS DIKSVLGYDQT
Sbjct: 1   MVAGGGISGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAILNFFGYFMIWLAVTRRISAPKVW 120
           TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGA++NFFGYFMIWLAVT +ISAPKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTGKISAPKVW 120

Query: 121 QMCVYICIGANSQSFANTGSLVTCVKNFPESRGVVL 157
           QMC+YICIGANSQSFANTGSLVTCVKNFPESRGVVL
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVL 156

[Close]

BLAST of Bhi07G000698 vs. ExPASy TrEMBL
Match: A0A6J1FZF2 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111449326 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.3e-70
Identity = 138/161 (85.71%), Postives = 146/161 (90.68%), Query Frame = 0


Query: 1   MVAGG-----GILRCSHIRSIAVHVITGRWFVVFASLLIMATTGATYMFGLYSDDIKSVL 60
           MVA G      I   S+IR+IAVH+ITGRWFVVFASLLIMA  GATYMF LYS+DIKSVL
Sbjct: 1   MVAAGQGGDRAISGSSNIRTIAVHIITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL 60

Query: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAILNFFGYFMIWLAVTRRIS 120
           GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGA+LNFFGYFMIWLAVT+RIS
Sbjct: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS 120

Query: 121 APKVWQMCVYICIGANSQSFANTGSLVTCVKNFPESRGVVL 157
            PKVWQMC+YIC+GANSQSF NTGSLVTCVKNFPESRGVVL
Sbjct: 121 TPKVWQMCLYICVGANSQSFVNTGSLVTCVKNFPESRGVVL 161

The following BLAST results are available for this feature:

BLAST of Bhi07G000698 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39210.1	3.7e-66	80.56	Major facilitator superfamily protein	[more]
AT2G28120.1	3.0e-60	77.70	Major facilitator superfamily protein	[more]
AT5G14120.1	4.0e-28	43.15	Major facilitator superfamily protein	[more]
AT1G18940.1	5.2e-28	40.65	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT5G50630.1	2.6e-27	42.03	Major facilitator superfamily protein	[more]

BLAST of Bhi07G000698 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I9E1	1.1e-14	35.56	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ...	[more]

BLAST of Bhi07G000698 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CFW3	1.5e-75	93.59	Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3BG09	1.5e-75	93.59	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 ...	[more]
A0A6J1GZI8	1.7e-74	91.67	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1K186	4.9e-74	91.67	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489...	[more]
A0A6J1FZF2	3.3e-70	85.71	protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111449326...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 24..156 e-value: 2.2E-61 score: 207.5
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 10..156
None	No IPR available	PANTHER	PTHR21576:SF117	MFS TRANSPORTER	coord: 10..156
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 24..153

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi07M000698	Bhi07M000698	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0099587	inorganic ion import across plasma membrane
biological_process	GO:0002239	response to oomycetes
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0022857	transmembrane transporter activity