Bhi07G000337 (gene) Wax gourd (B227) v1

Overview
NameBhi07G000337
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionPolcalcin Jun o
Locationchr7: 17202553 .. 17207876 (+)
RNA-Seq ExpressionBhi07G000337
SyntenyBhi07G000337
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCTAAATTTAATGAAACATTTAATTTTATAAAGCAATCTAGTCAATCCTCTAATGTTGTCATACAGATCTTACAACTTATTCGACATAACATCGATTTGACATAGAACATAAACGACCCAATACACATCACAACACATGAAACATGCACCATTCATCATCATCATGTCTTCATACATTCTCCCACAGTATATGGGGAAAAAATAAATAAATAAAAATAAATCTCAACCTCAATTAGTGCCATTTGTTAATCTCTCTCTCTTCCTTCCCTTTCTCTATATTTGGTAATCAACAAATTGAAACCAATCTGAAATCCCCATTTTTCCTTCACCGCTTCAATTTTCCCCCAATCCATAATGTTCCTTTTCTCCTTCCTGTTCATCCTCAATCCTCCGATTTTCAATTCCTTCTGATTTATCGGTTTTGGTGTTGCAATTAATCGATTGTTCTTCGCAGCAGAGAGAGAAATCCAACCATGGGATTCAGATCTCTGTTCCGTAAAAAGAAGCCGTTCTCCTCCGTCAATTCTCCTCTCGTTTCCTCATCCTCCACCCTCGGAACTCGATCCTCATCGGACGATTTACAAGCTCAGATCGCCGAACTCGAGGAAGTATTCAAGAAATTCGATGTGAACGGCGACGGAAAGATCTCCTCTGCCGAATTAGGCTCGATTATGGGAAGCCTAGGGCACAACGCTACGGAGGAAGAACTCGCGAAGATGATCGAGGAATTCGACGCTGATGGTGACGGATTCATTAATTTGCAGGAATTTGTGGAGTTAAATACTAAAGAAATCGATCCGGAGGAATTGTTGGAGAATCTGAGAGAGGCGTTTTCAGTGTATGATATCGATGGAAATGGATCGATCTCTGCCGAGGAATTGCATAAGGTTCTGAGGAGTTTGGGGGATGATTGCTCGGTAGCGGATTGCCGGCAGATGATCAGTGGCGTCGATAGCAACGGCGACGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGTCGACGGGACTCTCCGTCGCTGGCGCCTGAGATTTATCGTCCGGCGGCGGATTTCAAGAGGAATGTGCATATAATTTGCCAGAATTTGGATCCTTTTTTTTCCGTTCTGTTCTCTGACAAAATGTATTGCAAGTTCTTCCACTATCTTTTTTTTCTCTTTCGGGATTTTATCATCATTTTTTTCATTTTTTTAAAATAAAGTCCCTATTTTGTTTTTCTTTTATTAAAATGAGATGTTGTTCATTGTTGCATTGATTTATTAGCCACATGTAATGTTTTATATTATTTTTCTATGTAAAACTTTTACCTACAATTTCATGTTTAGTAAAAATTTTACATTTGATTTACTAGTGCCAAGCAATAATAAATATTTAGAATATATTTATTTGGTTAATTTATCTTTTTATTTTTTAGAAACAAGTTAAATTATCGTTTTAATCTCTAGAATAGTCTTTTGTTTCAATTTAATTATTTTAATTCTATTCTTATGGCTTGGTAAAAATAAAAAGATTTTTATTATTTACCTAATTAATATCGGATGTTTTCACACATTCACCCTTTTATTTATGGGTAGATTTTGTTTTTAGAGGAATAACAACAATCGGATTAAATCAAAGGCAAATTTGCCCCCACATATCCTTTTCAACCCCCACGATGTGGGTAAGCCAATCAGGAAAACTATGAGTCCAAACCACAGAAGTTATATCTCTAATGGCATAGGCAACCAGATTGAGGCCCTCTACTTGAAGCCTAATCAACAAATGATGAATTTCAACATAAATAACACCCCAAGGGGAAAGGACAAAGGTTGAAGGGAGAAATTTCTTGACAACCACCACTGTATCAGACTCGATCCTCAGTCAAACGGGCGGCAACACCGCACAGAAAATCACCGATTGGAAGCTATCAACCAAATTACAAAACGTAGGTACAACCCAATTCCATTTCTTAGAACCCCTTTTACAAACAAAAGCACGATAATTGTGAGTTGGTCTAGTGGTGGTGGAAATATCAAAAAAATTGTCAAAGGTCAAGGGTTCATGTGTTCTCGATCTATGGTGGTCACCTACCTAGGATTTAATATCCTATAGATTTCCTTATACCAAAATATTATAGGGTCAGACAGGTTGTCTTGTGAGATTAGTCGAGATATACGTAAGCTGAAAAAGCCTCCCTATTTGTCTGCGCAAATAGCTTATAAATGCTACTCGTGGAAAAAGAATCCTTGATTTAGTGTGTGATGCCAAAACCAAGAATTTGGGATCATAGTTAGAAATGTCCAAACAACTCGTGATTAAGGGGAATTCCCAATACACAAGTAATGGGAGAGGGAGTGAGAATTTTTTCCCATTTACAATTCGGGGCCGAGAATGGGGATTATATTCCCCATCCCTGGCCTCGGCCCCGTCCTACCCCGCCCCGCTTTTAGGGATAGCAAATAAGTTGTTCCCTTAAGTACTGTTTCCTAGTCTTGAACAAGAGGCCCTACCCTCTCTATAACTGAGAGGGACTCGGTTTATTGGTGGAACCATAAACCAATTATTCATTAGGGGATCAGTGGAGACTTAAGAAGCAATATGTATTACAAAGGTAAAATGGTATTTTGACCTAGCTGTAAATACGAACAACCTGTGTAGGATCGACTAACTGATTATGGTTAAATCAAGTGGACATAAATATATCTACAATGAGAAGAGTGCAACTATTGGGTTTTAGTGGAGTGTCCCAGTAGTTAACGGATGTTGGTTAAGTTGGTTAAAAGTGTTTCGCCGATTAGTCTCGGATCGTTGGAGCTTTAGGTCCCTCTACTAGCTCGTAAATTGATTAAAAAGAGTGTTGAGAGAATTTGAAATGTTCAAATTCGAATTAGGGTTTGGATTATTATATTTGATATAATTAACGTTTAATTATTGAATTAAACAAAATTGGGGATTTATAAATATTTAAGTTTAATTTATATTATATGAATAAGGATTCATTTTATGGAATATGTGTTTTATTAATTTAATATTAGATATTGAATTAAAATTAATTAAATTTGTTTAATTAATATTTTAATGAAACTAACTAATATTAATTTTATTTTAAATTAAAAATTATTTTTATTTAAAATCATTTTTTATAATTAAAAATAAGAAAAAGTGAGAAAATCCAACTTTTCTCACTTTCATTTGTTAATTATTATAGTAAAACACCTATAATTTCACTATTTGGCTAGGTGTCAATTTGTGATTAATTTTATTTGCATGATCTCATTGTATAAATAGTTATTTTAATTTATTTTTTATCTTCATGCAAATTTTAGAATTGTGAAGAAAATATTTGTTTTCTCTCAATTGATCTCTCCAAAGTTAAACCCTCCTCCAATTTGCTCTCAATTTGGGTTCCACCACCCAATTTAAGGCCAAGAGTGTAGTTGAGAGGCTCTTGTGGTCGTCTATATCACGGATACTTGAAGCAATTTGCAAAGGATTTGAGCTACTTAGGGATTCTTCAAAGGTTATACCTTAAACCCTAATTAGGTTTAATTTTTTTTTTTTTTGAATGTTTTTCCATGAAATTAATGTAATTAGAGTGTTTATAGTCCAATTAGCTTCTGCTATCATGTTTGTATCTCTATCAAATGTAATCTCGGGGTAAAACAACTTAACCGTTATTACGAATAAACTTGTGAAGGGTTGACTTACTAATTATAGTTAAACTAAGTGGATAGAAATATATCCACAGTAAGGGGAGTGCAACTACTGGCCTTTAGTGGAGTGACCCGGTAGTTAACAAATATTGGTTAATTAGGTTAAAAAGTTTAATCAGTTAATCTCGGATTGTTGGAACCCATGATCTGTAGGTCCATTAGGTCCTCCTACTAGCTCACATCGGACTAAACCTTAGAACAGTGTGACAAACGAGTTCAAAGTTTTTGAATTATATTTTTGGAGTAAAGTATTGAATATATCTAATATATATTTAACCTAATGTTTAATTATGAGTTAAACATATAGAGGGAGAAAAATATATTTTAAAAGATATTTTTTGGTTAATTTTTGAATTGTTGGGTATTTATTCATGTGATGAATATTTGTGTACTAAATTGGATGTTTGGTTTTTGGCATAATTTATTTCCTTCTATTTTGTAATAATTATTATAATTGTCAGGTGTTTAATGGGCATTAATGCCTCTAGAACGGTCCGTAAATTTGCTAGAGTCGTTAGAGTTGTTTTAAGATTGTAATCGAACAGTTTCGCAAAGAGGGCAGCTTCAATCTTAAAGAGAAAAGGCTTCCAGACCCATTTTCTCCTCCCATACTCAAAGAGCTACTCCAGACCTGACCTGTGACTCATGACTTGACTTGATTCATGAAGACTCCCCACCTGCGCGTGCCCCAGATCTGACCCCCGATCCGACTTTCAACCCATCAACCCACGCATTCCTCGACACATGATCCGACTTCTAATTCGTAAGCTCACGTGTGCCCAGATAGAGACGGCTCAGGCGGTTCAGATGGTCTAGATCGGTTCAGTTTGCTAGTTTTGGTTGGTTTTGGCTAGTTTTAGTCAGTAATGGCTTGTAGAAATACAAGCTATTGCAGCCTTTTACTTAGAATAATCAGGGTTGATGAGTAGAGCATGCATTGATATTGCTAAGAATTCATGTGAATAATGAACTTGCGTTGATCTGCACTAAAAATCCCTACTAACCCCATATCATGCATTATCTTGCGTCAAAGTGTCTATTTTGTAGTTATCGCAGGAGTCGACCACATGCGTTGATCTTAATGAAGCAAAGTTGATCGCATGCGTTGATCACAATGAAGAAAAGTTGGTCGCATGCAATTGCGCAACTACAATAATAGCCATGATAGAGGTATGATCGCAAAGAGGGGGTGGAATGCGTTGATCCTTCAGAAGAAACGATTATGAATGCATACCTCGGAAGCATCAAAGCGATAAGATCATGATGACATTCTCACCTACCGCGTTAGTAATGGTAGTGATTCAGAGCAGGATTATCAAACTTGTCAATGTTTGGCTCTATAAATAGAGCCTTAGAGCCCGAGTTCAAGCATTCGGGATCTTCTACCTTAGGCAGATTCCTATGATAACAGATGAGAGCATGAGCAAGATTTTCTTTCAGAGTTCTTCATTTCCACAACTTCCTCTGATTGATAACCGAATCTTCGCTGGAGATAGAGCTTGAGAGGGACTACTCCACCATCCATCCATTGCACCAACGGCAGCCTTGACATACATCTGATAGAAGCAAGAGATTGCCTACATCTAGCCTTCCATCTCTCTTCTCACCTCTGTATTGTATTACATTTTGG

mRNA sequence

GCCTAAATTTAATGAAACATTTAATTTTATAAAGCAATCTAGTCAATCCTCTAATGTTGTCATACAGATCTTACAACTTATTCGACATAACATCGATTTGACATAGAACATAAACGACCCAATACACATCACAACACATGAAACATGCACCATTCATCATCATCATGTCTTCATACATTCTCCCACAGTATATGGGGAAAAAATAAATAAATAAAAATAAATCTCAACCTCAATTAGTGCCATTTGTTAATCTCTCTCTCTTCCTTCCCTTTCTCTATATTTGGTAATCAACAAATTGAAACCAATCTGAAATCCCCATTTTTCCTTCACCGCTTCAATTTTCCCCCAATCCATAATGTTCCTTTTCTCCTTCCTGTTCATCCTCAATCCTCCGATTTTCAATTCCTTCTGATTTATCGGTTTTGGTGTTGCAATTAATCGATTGTTCTTCGCAGCAGAGAGAGAAATCCAACCATGGGATTCAGATCTCTGTTCCGTAAAAAGAAGCCGTTCTCCTCCGTCAATTCTCCTCTCGTTTCCTCATCCTCCACCCTCGGAACTCGATCCTCATCGGACGATTTACAAGCTCAGATCGCCGAACTCGAGGAAGTATTCAAGAAATTCGATGTGAACGGCGACGGAAAGATCTCCTCTGCCGAATTAGGCTCGATTATGGGAAGCCTAGGGCACAACGCTACGGAGGAAGAACTCGCGAAGATGATCGAGGAATTCGACGCTGATGGTGACGGATTCATTAATTTGCAGGAATTTGTGGAGTTAAATACTAAAGAAATCGATCCGGAGGAATTGTTGGAGAATCTGAGAGAGGCGTTTTCAGTGTATGATATCGATGGAAATGGATCGATCTCTGCCGAGGAATTGCATAAGGTTCTGAGGAGTTTGGGGGATGATTGCTCGGTAGCGGATTGCCGGCAGATGATCAGTGGCGTCGATAGCAACGGCGACGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGTCGACGGGACTCTCCGTCGCTGGCGCCTGAGATTTATCGTCCGGCGGCGGATTTCAAGAGGAATGTGCATATAATTTGCCAGAATTTGGATCCTTTTTTTTCCGTTCTGTTCTCTGACAAAATGTATTGCAAGTTCTTCCACTATCTTTTTTTTCTCTTTCGGGATTTTATCATCATTTTTTTCATTTTTTTAAAATAAAGTCCCTATTTTGTTTTTCTTTTATTAAAATGAGATGTTGTTCATTGTTGCATTGATTTATTAGCCACATGTAATGTTTTATATTATTTTTCTATGTAAAACTTTTACCTACAATTTCATGTTTAGTAAAAATTTTACATTTGATTTACTAGTGCCAAGCAATAATAAATATTTAGAATATATTTATTTGGTTAATTTATCTTTTTATTTTTTAGAAACAAGTTAAATTATCGTTTTAATCTCTAGAATAGTCTTTTGTTTCAATTTAATTATTTTAATTCTATTCTTATGGCTTGGTAAAAATAAAAAGATTTTTATTATTTACCTAATTAATATCGGATGTTTTCACACATTCACCCTTTTATTTATGGGTAGATTTTGTTTTTAGAGGAATAACAACAATCGGATTAAATCAAAGGCAAATTTGCCCCCACATATCCTTTTCAACCCCCACGATGTGGGTAAGCCAATCAGGAAAACTATGAGTCCAAACCACAGAAGTTATATCTCTAATGGCATAGGCAACCAGATTGAGGCCCTCTACTTGAAGCCTAATCAACAAATGATGAATTTCAACATAAATAACACCCCAAGGGGAAAGGACAAAGGTTGAAGGGAGAAATTTCTTGACAACCACCACTGTATCAGACTCGATCCTCAGTCAAACGGGCGGCAACACCGCACAGAAAATCACCGATTGGAAGCTATCAACCAAATTACAAAACTTATCGCAGGAGTCGACCACATGCGTTGATCTTAATGAAGCAAAGTTGATCGCATGCGTTGATCACAATGAAGAAAAGTTGGTCGCATGCAATTGCGCAACTACAATAATAGCCATGATAGAGGTATGATCGCAAAGAGGGGGTGGAATGCGTTGATCCTTCAGAAGAAACGATTATGAATGCATACCTCGGAAGCATCAAAGCGATAAGATCATGATGACATTCTCACCTACCGCGTTAGTAATGGTAGTGATTCAGAGCAGGATTATCAAACTTGTCAATGTTTGGCTCTATAAATAGAGCCTTAGAGCCCGAGTTCAAGCATTCGGGATCTTCTACCTTAGGCAGATTCCTATGATAACAGATGAGAGCATGAGCAAGATTTTCTTTCAGAGTTCTTCATTTCCACAACTTCCTCTGATTGATAACCGAATCTTCGCTGGAGATAGAGCTTGAGAGGGACTACTCCACCATCCATCCATTGCACCAACGGCAGCCTTGACATACATCTGATAGAAGCAAGAGATTGCCTACATCTAGCCTTCCATCTCTCTTCTCACCTCTGTATTGTATTACATTTTGG

Coding sequence (CDS)

ATGGGATTCAGATCTCTGTTCCGTAAAAAGAAGCCGTTCTCCTCCGTCAATTCTCCTCTCGTTTCCTCATCCTCCACCCTCGGAACTCGATCCTCATCGGACGATTTACAAGCTCAGATCGCCGAACTCGAGGAAGTATTCAAGAAATTCGATGTGAACGGCGACGGAAAGATCTCCTCTGCCGAATTAGGCTCGATTATGGGAAGCCTAGGGCACAACGCTACGGAGGAAGAACTCGCGAAGATGATCGAGGAATTCGACGCTGATGGTGACGGATTCATTAATTTGCAGGAATTTGTGGAGTTAAATACTAAAGAAATCGATCCGGAGGAATTGTTGGAGAATCTGAGAGAGGCGTTTTCAGTGTATGATATCGATGGAAATGGATCGATCTCTGCCGAGGAATTGCATAAGGTTCTGAGGAGTTTGGGGGATGATTGCTCGGTAGCGGATTGCCGGCAGATGATCAGTGGCGTCGATAGCAACGGCGACGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGTCGACGGGACTCTCCGTCGCTGGCGCCTGA

Protein sequence

MGFRSLFRKKKPFSSVNSPLVSSSSTLGTRSSSDDLQAQIAELEEVFKKFDVNGDGKISSAELGSIMGSLGHNATEEELAKMIEEFDADGDGFINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGLSVAGA
Homology
BLAST of Bhi07G000337 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 210.3 bits (534), Expect = 1.3e-54
Identity = 109/163 (66.87%), Postives = 128/163 (78.53%), Query Frame = 0

Query: 23  SSSTLGTRSSSDDLQ------AQIAELEEVFKKFDVNGDGKISSAELGSIMGSLGHNATE 82
           SSS +G  + S  LQ       +I ELE VFKKFDVNGDGKISS ELG+IM SLGH   E
Sbjct: 12  SSSNVGIGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPE 71

Query: 83  EELAKMIEEFDADGDGFINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEEL 142
           EEL K I E D  GDG+IN +EFVELNTK +D  ++LENL++AFSVYDIDGNGSISAEEL
Sbjct: 72  EELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEEL 131

Query: 143 HKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180
           H+VLRSLGD+CS+A+CR+MI GVD +GDG I FEEFK+MM+ G
Sbjct: 132 HEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMG 174

BLAST of Bhi07G000337 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 142.5 bits (358), Expect = 3.4e-34
Identity = 70/150 (46.67%), Postives = 107/150 (71.33%), Query Frame = 0

Query: 29  TRSSSDDLQAQIAELEEVFKKFDVNGDGKISSAELGSIMGSLGHNATEEELAKMIEEFDA 88
           +++ S +    + ++++VF++FD N DGKIS  EL  ++G+L  NA++EE   M++EFD 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 89  DGDGFINLQEFVEL--NTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLRSLGDD 148
           DG+GFI+L EFV L   + +      + +L+EAF +YD+D NG ISA ELH V+++LG+ 
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 149 CSVADCRQMISGVDSNGDGMISFEEFKVMM 177
           CS+ DC++MI+ VDS+GDG + FEEFK MM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

BLAST of Bhi07G000337 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 141.0 bits (354), Expect = 9.8e-34
Identity = 71/142 (50.00%), Postives = 103/142 (72.54%), Query Frame = 0

Query: 42  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHNATEEELAKMIEEFDADGDGFINLQEFVE 101
           ++++VF++FD NGDGKIS  EL  ++ +L   A+ EE   M+++FD DG+GFI+L EFV 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 102 LNTKEI----DPEELLENLREAFSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMIS 161
           L    I    +    + +L+EAF +YD+DGNG ISA+ELH V+++LG+ CSV DC++MIS
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 162 GVDSNGDGMISFEEFKVMMSTG 180
            VD +GDG ++F+EFK MMS G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

BLAST of Bhi07G000337 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 137.1 bits (344), Expect = 1.4e-32
Identity = 73/143 (51.05%), Postives = 98/143 (68.53%), Query Frame = 0

Query: 42  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHNATEEELAKMIEEFDADGDGFINLQEFVE 101
           EL++VF KFD NGDGKIS +ELG++  S+G + TEEEL ++++E D D DGFIN +EF  
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 102 LNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDS 161
           +        E    +REAF +YD + NG IS+ E+HKVL  LG  CSV DC +MI  VD+
Sbjct: 80  ICRSSSSAVE----IREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDT 139

Query: 162 NGDGMISFEEFKVMMSTGLSVAG 185
           +GDG ++FEEF+ MMS+   V G
Sbjct: 140 DGDGNVNFEEFQKMMSSPELVKG 158

BLAST of Bhi07G000337 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 133.3 bits (334), Expect = 2.0e-31
Identity = 70/137 (51.09%), Postives = 93/137 (67.88%), Query Frame = 0

Query: 42  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHNATEEELAKMIEEFDADGDGFINLQEFVE 101
           EL++VF +FD NGDGKIS  ELG +  ++G + TE EL +++EE D D DG+INL EF  
Sbjct: 23  ELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFST 82

Query: 102 LNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDS 161
           L        E    +R+AF +YD D NG ISA ELH+VL  LG  CSV DC +MI  VD+
Sbjct: 83  LCRSSSSAAE----IRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 142

Query: 162 NGDGMISFEEFKVMMST 179
           +GDG ++FEEF+ MM++
Sbjct: 143 DGDGNVNFEEFQKMMTS 155

BLAST of Bhi07G000337 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 1.9e-53
Identity = 109/163 (66.87%), Postives = 128/163 (78.53%), Query Frame = 0

Query: 23  SSSTLGTRSSSDDLQ------AQIAELEEVFKKFDVNGDGKISSAELGSIMGSLGHNATE 82
           SSS +G  + S  LQ       +I ELE VFKKFDVNGDGKISS ELG+IM SLGH   E
Sbjct: 12  SSSNVGIGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPE 71

Query: 83  EELAKMIEEFDADGDGFINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEEL 142
           EEL K I E D  GDG+IN +EFVELNTK +D  ++LENL++AFSVYDIDGNGSISAEEL
Sbjct: 72  EELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEEL 131

Query: 143 HKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180
           H+VLRSLGD+CS+A+CR+MI GVD +GDG I FEEFK+MM+ G
Sbjct: 132 HEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMG 174

BLAST of Bhi07G000337 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 1.8e-40
Identity = 94/180 (52.22%), Postives = 123/180 (68.33%), Query Frame = 0

Query: 5   SLFRKKKPFSSVNSPLVSSSSTLGTRSSSDDLQAQIAELEEVFKKFDVNGDGKISSAELG 64
           +LFR++    S   P    +S  G+ + + +      E+E VF+KFD NGDG+IS +ELG
Sbjct: 8   ALFRRRSGSKSPPLPQADPASGGGSPAPTPE-----EEMERVFRKFDANGDGRISRSELG 67

Query: 65  SIMGSLGHNATEEELAKMIEEFDADGDGFINLQEFVELN-TKEIDPEELLENLREAFSVY 124
           ++  SLGH AT++ELA+M+ E DADGDGFI+L EF  LN T   D   + E+LR AF V+
Sbjct: 68  ALFESLGHAATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVF 127

Query: 125 DIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGLSVA 184
           D DGNG+ISA EL +VL  LG+  +V  CR+MI GVD NGDG+ISFEEFKVMM+ G S A
Sbjct: 128 DADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVDQNGDGLISFEEFKVMMAGGGSFA 182

BLAST of Bhi07G000337 vs. ExPASy Swiss-Prot
Match: Q6L4D4 (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 GN=CML15 PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 2.3e-35
Identity = 74/139 (53.24%), Postives = 102/139 (73.38%), Query Frame = 0

Query: 42  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHNATEEELAKMIEEFDADGDGFINLQEFVE 101
           E E VF+KFD NGDG+IS AEL ++  S+GH  T++E+A+M++E D+DGDG+I+L EF  
Sbjct: 55  ETERVFRKFDANGDGRISRAELAALFRSVGHAVTDDEVARMMQEADSDGDGYISLGEFAA 114

Query: 102 LNTKEI-DPEELLENLREAFSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVD 161
           ++     D     E+LR AF V+D DGNG I+  EL +VLR +G+  +VA CR+MI GVD
Sbjct: 115 ISAPPPGDAAAAEEDLRHAFGVFDADGNGVITPAELARVLRGIGEAATVAQCRRMIDGVD 174

Query: 162 SNGDGMISFEEFKVMMSTG 180
            NGDG+I+FEEFK+MM+ G
Sbjct: 175 RNGDGLINFEEFKLMMAAG 193

BLAST of Bhi07G000337 vs. ExPASy Swiss-Prot
Match: O64943 (Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2)

HSP 1 Score: 144.8 bits (364), Expect = 9.6e-34
Identity = 77/160 (48.12%), Postives = 108/160 (67.50%), Query Frame = 0

Query: 22  SSSSTLGTRSSSDDLQAQIAELEEVFKKFDVNGDGKISSAELGSIMGSLGHNATEEELAK 81
           SS  +    S    ++  + ELEEVFKKFD NGDGKIS +EL  I+ SLG +  E E+  
Sbjct: 6   SSDGSKSACSGEVVMEQSVHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKA 65

Query: 82  MIEEFDADGDGFINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLR 141
           M+EE DADGDG+++LQEFV+LN K       +++L+ AF V+D D NGSISA EL   L 
Sbjct: 66  MMEEADADGDGYVSLQEFVDLNNKGAS----VKDLKNAFKVFDRDCNGSISAAELCHTLE 125

Query: 142 SLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGLS 182
           S+G+ C++ + + +I  VD NGDG+IS EEF+ MM++ ++
Sbjct: 126 SVGEPCTIEESKNIIHNVDKNGDGLISVEEFQTMMTSEMT 161

BLAST of Bhi07G000337 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 4.8e-33
Identity = 70/150 (46.67%), Postives = 107/150 (71.33%), Query Frame = 0

Query: 29  TRSSSDDLQAQIAELEEVFKKFDVNGDGKISSAELGSIMGSLGHNATEEELAKMIEEFDA 88
           +++ S +    + ++++VF++FD N DGKIS  EL  ++G+L  NA++EE   M++EFD 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 89  DGDGFINLQEFVEL--NTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLRSLGDD 148
           DG+GFI+L EFV L   + +      + +L+EAF +YD+D NG ISA ELH V+++LG+ 
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 149 CSVADCRQMISGVDSNGDGMISFEEFKVMM 177
           CS+ DC++MI+ VDS+GDG + FEEFK MM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

BLAST of Bhi07G000337 vs. ExPASy TrEMBL
Match: A0A5A7TBZ6 (Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold228G00230 PE=4 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.4e-88
Identity = 175/184 (95.11%), Postives = 178/184 (96.74%), Query Frame = 0

Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGTRSSSDDLQAQIAELEEVFKKFDVNGDGKISS 60
           MGFRSLFRKKKPFSSVNSPLVSSSSTLG+RSS D  ++QIAELEEVFKKFDVNGDGKISS
Sbjct: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKISS 60

Query: 61  AELGSIMGSLGHNATEEELAKMIEEFDADGDGFINLQEFVELNTKEIDPEELLENLREAF 120
           AELGSIM SLGHNATEEEL KMIEEFDADGDGFINL EFVELNTKEIDPEELLENLREAF
Sbjct: 61  AELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120

Query: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180
           SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL
Sbjct: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180

Query: 181 SVAG 185
           SVAG
Sbjct: 181 SVAG 184

BLAST of Bhi07G000337 vs. ExPASy TrEMBL
Match: A0A1S3CNQ0 (probable calcium-binding protein CML25 OS=Cucumis melo OX=3656 GN=LOC103502536 PE=4 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.4e-88
Identity = 175/184 (95.11%), Postives = 178/184 (96.74%), Query Frame = 0

Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGTRSSSDDLQAQIAELEEVFKKFDVNGDGKISS 60
           MGFRSLFRKKKPFSSVNSPLVSSSSTLG+RSS D  ++QIAELEEVFKKFDVNGDGKISS
Sbjct: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKISS 60

Query: 61  AELGSIMGSLGHNATEEELAKMIEEFDADGDGFINLQEFVELNTKEIDPEELLENLREAF 120
           AELGSIM SLGHNATEEEL KMIEEFDADGDGFINL EFVELNTKEIDPEELLENLREAF
Sbjct: 61  AELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120

Query: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180
           SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL
Sbjct: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180

Query: 181 SVAG 185
           SVAG
Sbjct: 181 SVAG 184

BLAST of Bhi07G000337 vs. ExPASy TrEMBL
Match: A0A0A0KX11 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G627080 PE=4 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 5.4e-88
Identity = 173/185 (93.51%), Postives = 179/185 (96.76%), Query Frame = 0

Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGTRSSSDDLQAQIAELEEVFKKFDVNGDGKISS 60
           MGFRSLFRKKKPFSSVNSPLVSSSSTLG+RSS DD ++QI ELEEVFKKFDVNGDGKISS
Sbjct: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS 60

Query: 61  AELGSIMGSLGHNATEEELAKMIEEFDADGDGFINLQEFVELNTKEIDPEELLENLREAF 120
           AELGSIM SLGHNATEEEL KMI+EFDADGDGFINL EFVELNTKEIDPEELLENLREAF
Sbjct: 61  AELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120

Query: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180
           SVYDIDGNGSISAEELHKVL+SLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL
Sbjct: 121 SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180

Query: 181 SVAGA 186
           SVAG+
Sbjct: 181 SVAGS 185

BLAST of Bhi07G000337 vs. ExPASy TrEMBL
Match: A0A6J1JSH4 (probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC111487142 PE=4 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 5.4e-80
Identity = 162/185 (87.57%), Postives = 177/185 (95.68%), Query Frame = 0

Query: 1   MGFRSLF-RKKKPFSSVNSPLVSSSSTLGTRSSSDDLQAQIAELEEVFKKFDVNGDGKIS 60
           MGF+SLF RKKKPF+SV+SPLVS+SSTLG+ SS +D +AQIAELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFKSLFCRKKKPFASVDSPLVSASSTLGSGSSLEDSRAQIAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHNATEEELAKMIEEFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           S+ELG+I+GSLGHNA EEELAKMIEEFDADGDGFINLQEFVELNTKEIDPEELLE+LREA
Sbjct: 61  SSELGTILGSLGHNAKEEELAKMIEEFDADGDGFINLQEFVELNTKEIDPEELLESLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKV-MMST 180
           FSVYDIDGNGSI+AEELH+VL+SLGDDCSV DCRQMI+GVD NGDGMISFEEFKV MMST
Sbjct: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMINGVDRNGDGMISFEEFKVMMMST 180

Query: 181 GLSVA 184
           GLSVA
Sbjct: 181 GLSVA 185

BLAST of Bhi07G000337 vs. ExPASy TrEMBL
Match: A0A6J1FS86 (probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC111447972 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 7.0e-80
Identity = 162/185 (87.57%), Postives = 177/185 (95.68%), Query Frame = 0

Query: 1   MGFRSLF-RKKKPFSSVNSPLVSSSSTLGTRSSSDDLQAQIAELEEVFKKFDVNGDGKIS 60
           MGF+SLF RKKKPF+SV+SPLVS+SSTLG+ SS +D +AQIAELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFKSLFCRKKKPFASVDSPLVSASSTLGSGSSLEDSRAQIAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHNATEEELAKMIEEFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           S+ELG+I+GSLGHNA EEELAKMIEEFDADGDGFI+LQEFVELNTKEIDPEELLENLREA
Sbjct: 61  SSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKV-MMST 180
           FSVYDIDGNGSI+AEELH+VL+SLGDDCSV DCR+MISGVD NGDGMISFEEFKV MMST
Sbjct: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRRMISGVDRNGDGMISFEEFKVMMMST 180

Query: 181 GLSVA 184
           GLSVA
Sbjct: 181 GLSVA 185

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G24620.11.3e-5466.87EF hand calcium-binding protein family [more]
AT1G66400.13.4e-3446.67calmodulin like 23 [more]
AT5G37770.19.8e-3450.00EF hand calcium-binding protein family [more]
AT1G73630.11.4e-3251.05EF hand calcium-binding protein family [more]
AT1G18210.12.0e-3151.09Calcium-binding EF-hand family protein [more]
Match NameE-valueIdentityDescription
Q9FYK21.9e-5366.87Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ... [more]
Q8RZB51.8e-4052.22Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q6L4D42.3e-3553.24Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
O649439.6e-3448.13Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2[more]
Q9C8Y14.8e-3346.67Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ... [more]
Match NameE-valueIdentityDescription
A0A5A7TBZ62.4e-8895.11Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CNQ02.4e-8895.11probable calcium-binding protein CML25 OS=Cucumis melo OX=3656 GN=LOC103502536 P... [more]
A0A0A0KX115.4e-8893.51Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G627080 PE=4 SV=1[more]
A0A6J1JSH45.4e-8087.57probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC1114871... [more]
A0A6J1FS867.0e-8087.57probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 33..53
NoneNo IPR availableGENE3D1.10.238.10coord: 108..181
e-value: 3.1E-25
score: 90.3
coord: 16..107
e-value: 1.3E-25
score: 91.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..34
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..34
NoneNo IPR availablePANTHERPTHR23050CALCIUM BINDING PROTEINcoord: 24..179
NoneNo IPR availablePANTHERPTHR23050:SF382CALCIUM-BINDING PROTEIN CML25-RELATEDcoord: 24..179
IPR002048EF-hand domainSMARTSM00054efh_1coord: 115..143
e-value: 6.4E-6
score: 35.7
coord: 151..179
e-value: 4.0E-5
score: 33.0
coord: 42..70
e-value: 4.7E-7
score: 39.4
coord: 78..106
e-value: 5.4E-5
score: 32.6
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 42..102
e-value: 2.4E-13
score: 50.3
coord: 114..176
e-value: 4.6E-13
score: 49.4
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 147..182
score: 12.086506
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 111..146
score: 15.517639
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 74..109
score: 13.564962
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 38..73
score: 16.24292
IPR002048EF-hand domainCDDcd00051EFhcoord: 116..177
e-value: 2.98786E-20
score: 77.9733
IPR002048EF-hand domainCDDcd00051EFhcoord: 42..102
e-value: 2.33303E-20
score: 78.3585
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 87..99
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 124..136
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 51..63
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 160..172
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 38..177

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi07M000337Bhi07M000337mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048767 root hair elongation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005509 calcium ion binding