Bhi06G001544 (gene) Wax gourd (B227) v1

Overview
NameBhi06G001544
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionCCHC-type domain-containing protein
Locationchr6: 50693131 .. 50698150 (-)
RNA-Seq ExpressionBhi06G001544
SyntenyBhi06G001544
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAGGACAACGCCGCCGTCGCAGCGCCGACGGGATGCTACAAGTGCGGGAAGCCGGGCCACTGGTCTCGTGATTGCCCTTCCTCCGCTCCAAATCCCAATCCCACTTCCAATTCTTCTAGACCTGTCTCTTATACACATCTAGATGTGTATAAGAGACAGGGTCTCGTGATTGCCCTTCCTCCGCTCCAAATCCCAATCCCACTTCCAATTCTTCTAGACCTCCTTTCTCCTCTGCTCCTGCTGCCCCTTCAAAAGTTGCAGAGAAGCCCAAGAAAGCCCCCAGAACCAGGCCCAAGCTCACTCCTGAGCTACTCCTTTCTGAGGATGGTCTAGGTTACGTCCTTCGCCATTTTCCTCGCGTTTTCAAGTATCGCGGCCGTGGACACGAGGTTATCTTCTATTTTCCTTCCGATTATCGTGTGAATAGTCGATTTGTTCTGATCAGGCACATGCAAGTTTTATTTGAGATATATTTTCAACATTGCTGCTCAAGGACAAAGAGGGTTTTCGAAAACAAAATACTAATTACTAGAGTCTGACGCGTTTTAGGATTTAGGTTTTTGATATGAAATCGATAATCAAATTAAAACGAAAACAGTAGAGTAACTAACATAGAGATTTAGATGGTTCACTAACTTGTTGGCAACGTCCATGAATGGAGGGAGCGATAAATGTCAGATTACAGACCCCCACCAGCCCGTGTTGCCTCTGGTCCCTGACTCATTGACCGGTCAGTGTTGAGAATCCCATATTGAAAAAATTAAGGGACCTCATACTCCTTATAAGTTAAGTTTGCTAGATTCAAGGCATTCCAACGTATTTTGTGTTGAATGATTAGAAACAATTTTGAATGTGATGCATATTTCATTTGAAGATTTGTTCGAGAGAGCTTAGGCTTCATCGGGGGAATAGGTATTTACCTTAATGGTTTGTTTATTGGAAAACCAGATTTAAACCTTTTTCCATTGAATGGTAGCTATGAGTGTTTCATCAACACTATGTATATGTATATTTTCTTTTTCTTATTACCGTGCCATCCTCTCATCTATTCTCATTTTCTAATGATTTGATTATTGTACTGATTGAGTTATGCTTTGATTGCTGTTTTGTAGATCAGTGATTTGGGAAACCTTATTGGCTTATACAGGGAATGGCATTCTCATATACTCCCTTATTACTCTTTCGAGCATTTTGTACATAAGGTGGAACAGGTAGCTGCTACAAAACGTGTAAAGGTAAAAATGTGCATGAACCCTGAATTTATTTGTTCTTTACGATGAAGGTATCAAAGAGATGTACATATATGGCTAGCTGGATTGGTATGTGTGTCAAAGTACAGGAACTTAGCCTAATAACGTACTAATATAGATAGCATTTGAACTAAAGGAACTGCGCTTAATTAACCCAGTGCATCTGAAGGGAGTAGAGACAGAGTGCATCATTTTTTGTTGGCACAGAATGCGCTCAAACTCTGTAATATTTTAAGTGAATCCTTATTTATTGAATCAAATCAACATGGAGCAAATTATTAAATGATATCTAATTTACAATGAATTTGCATTCTTTCTAAAAAGACATATTTTGAGCAGATGTGCCTTGGAGAACTAAGGGAGAGGGTTGCCAGGGGAGGGGATCCTACAAAGTGGCGCGAAGCACCCATTTTAAACGATGATGATGTCAATAATGAAAAAGGTACCTTCTAGTATTATCATTTATTAAAAGATTGTAAGGCCCCTAGCTATAGAAATAATAGGAGTATTAGTAGAATATTAGTATGGTATTAGTAAGGGATATAGGGGTAATTAAATGGGAAGTTAGTTACTGAATATTTCTATAAATAGGAGAGGAAGTATTGGGAGGGTGTGAAGAACTTTATGTGAGGATTTCCTAATTGAGAATTTGGGAGAGACTAGCCCTCTCGAAAGGTTAGGGTTTATCTTGTTATTTTGTTTGTGAGTTTATAGCATATTTCAATACATTTTCCTATATTTCTGTAATTGAATGGTCCTTTAGTTTTTCTATATTTTTTAGTACTGATCTTGTTAGGAGGGTTCCTAACAAAGACACTCGTATTATTGTTTTGATCAGCTGTTTTGTCATGATAGAATGTATGCCATTAAATTGTGTTAGTGTTTGGTGTGTTGTGCATTGTCCTCTAACTGTATTTAGAACATTTAAATTTTGTTTAGACGATAAATTTGAAGTTACCGAATTACCAAAACAAGTAAGCAAGAAACAATTGTAAAAATGAAAGAAAGGTTGAAATTGAACACGATGATTAGTTTTTAGCGTTCCATTTTGATCTCTAAAAGTTTTTGTAGTGTCTCTAAAAGTAGCCATGTCTATAGCTATGAGAATTATAGGTTTTAAGAAGATTAATATCTATAAACATTGAACAACACATTGTCCTAATTCTTGTCAGGGCACTCAAGTACGTGTACCTGATGTAAATATGGACATAGTATATGATGTAGGATTTTAGGTGGACATTGCTTGAGTTAACGATGCCCATAAAGAGCCTACATTACATACAGATTTACAAAACTTCTAGAGATTGGTAAATGCAGCGTGATAATAGGATATGGGACACTGATTTCATTCAGGACAAGTAGCTTATGTTGACAAAAGGAGCCCAATTTTTCAGTATATGAGTGTGATTGTGTATGCTTATGTGCGTTTTCTGTATCTATTGAAATGCTTGGATTATTTGACTATAAACATTGAACAACACGAATAAAAGTTATACTTGGATTATTGTTATTTTTTATGTTCCACTCCTATCAAGACAGGGTTTCGTGAAAGCTTCCAAATACTGCTCATTCAAATTGTCGTCTTCAGATTTGCGTTGGAAGATCCTTCAATATTGGTTCCTCTTTTTGTGGGCTTCTCAAAGAAAGTTCATTATTTTATTTAAAAACGTTGAGGATACCACATTAAAAAGTGAAGAAACTTACCCTCTTTATAAGATATACAAAGTTATTCATCTCGTTGCCAATTGGTTTTGAGATGAAACATAATGTTTCTCTAATATGGTATTATTATAGCTCATAAATCCCAAGTTTGGACAAAAAAAAGGGGGGGATTTCATACAAGAATAGGGAATCCGAAGAGGCATCATTTTAAGAGGACATGTTGAGGACCCCACATTAGAAAAGTCGATAGACCTCAAAATCTTTATAAGATACATGGGTTACCCTCTCATCGATCAATTTGTTATGAGATGGAACCCCATGTGAAAAAAACTTCTCATCTTTCTCTCTTATACATATTTGTATGTTGTTGATCAAGGCTATGAGATTTGAGAGCTGTGTCTATGTTGGGAATGTGATCAAAATCTCACATTGGCTAGATAAGCGTGAGTATATAATTGAAGGACAATATCTCTATTGGTATGAAGCAGCCTTTTGGGTGATTTCAAAAGCATGTCTTGAGAGCTTATGCCTAAAATGGACAATATCATACTATTATGGAGATATTGGAGAGTCATTATTCCAAACAATATACACCTTTGTCAATTGGAGCTTAGTTCTAAATGTTTTCTCACGACCTTACTTGAACAGGATCTGTTCTATAGGTTGTACAACTATGTCCTTGAGTTCTAAGTTCTAAATGTTTCCTCTTCTCATTGGCCTCTGGATGCCCAGTTTGGTATGCACTAAGCAGTTGGTGCTTGGAATGGGAAAATTGTATCACAATGTAGGTAGTAAATATGTTGCCTTTTTCATTTTCTTTATACCTTGTGCCATGGTATGTATCTTGGCTCTATGTAGTTAGATATGCAACCTAGGAAGTTAAATCCATCCATTTGAGTATTTTCTCTCATGTGTGGAACTATACTACGATGTGATTGGAAAAAAAAAATTTAGATTCTAAAACTAATCATTATTTGAGCACCCCTTTTCATGAACTTCAAGTCCGTTTGCCAATGCTTGCAGAAGCCAAAGACCCGGAGGGCATATTTGATAATCAGGAAGAGGCTTTGAGGAATAATGGTGCTGATGACATTGAAGAGGACATGCTTCAGGAAGTTTATGAGAAAGCTACGGAAGTAAGTCTTTCTTGGTTCATTTTTTAACACTTGGTTGCTTTCCATCCCCTATAATTCTTTTAGTTTTAATCGTACCATTGCTTAATGTGAAAACTCTCGCAGTTAATTGAATGGGGAATTTTACCGTGAGTCTTAAATTAGATTTCAGGAATAGTCATATTGAAGCAAACTTTTCAGTCTTTATTGGCAGATCAATTTTCCAGATGAAGTTGTCAGGTCGATTATTAAAAATCTATATGTTGCCCCAATTAACTATAATTTACACTTGAACTCAATTTTCTAAACCAATTTAGATAATTAATCTATTTTCACTCTGTTGCACTTAAAATCAACTCAAAATCACATACATATAATTATTCTTCTTCCACCCCATAGAAGTAGCCAAAAAAAAAAAAAAAAAAAAGAAAGAAAACTGTCACGTGATTCTGAACAAGTAGAGGGAAATAGAGCAATGCAAGAAGATGAAAAGTCACTCAGAATTATTGAAAAAGCACTACCATCTATTTCATTACTCAAGTGTGAATGTTATGTATTAAACGGTTTTTACTTAACTATGGTAGTCTTAAAACGGTTTTTACCTAACTATGGTAAAAAGTAAGCTCTTAGGTTGAAACCCTTATCTGCATACTAATGTCTATAGTTGATGAGAAACTTGAAGCAAGTATACCTCCATCCGTATTCTTTCTAACTTGAGTTTGATCACACCTAACACCATTTCATGGCATATTAACGATCTACAACATTTGGTTACGGGTGTTTACATGTTTGTAGGAACCATCGCAGAATGTACAAAGTGATTCCTTCACCATTGGTGAAACTATAGAAGGTGTGGCCAACCAAGAACCAAAGATTAGTAATTCAAGTGAAACTCAACTCACCGAGGAACAGAGAGGTCGAATGGAAGCCAACAGGTTGAAGGCATTAGAAAGAGCTGCAGCCAAGGCTCGCTCTTTGCCCACTGTCTGA

mRNA sequence

ATGGAGAAGGACAACGCCGCCGTCGCAGCGCCGACGGGATGCTACAAACCTCCTTTCTCCTCTGCTCCTGCTGCCCCTTCAAAAGTTGCAGAGAAGCCCAAGAAAGCCCCCAGAACCAGGCCCAAGCTCACTCCTGAGCTACTCCTTTCTGAGGATGGTCTAGGTTACGTCCTTCGCCATTTTCCTCGCGTTTTCAAGTATCGCGGCCGTGGACACGAGATCAGTGATTTGGGAAACCTTATTGGCTTATACAGGGAATGGCATTCTCATATACTCCCTTATTACTCTTTCGAGCATTTTGTACATAAGGTGGAACAGGTAGCTGCTACAAAACGTGTAAAGATGTGCCTTGGAGAACTAAGGGAGAGGGTTGCCAGGGGAGGGGATCCTACAAAGTGGCGCGAAGCACCCATTTTAAACGATGATGATGTCAATAATGAAAAAGAAGCCAAAGACCCGGAGGGCATATTTGATAATCAGGAAGAGGCTTTGAGGAATAATGGTGCTGATGACATTGAAGAGGACATGCTTCAGGAAGTTTATGAGAAAGCTACGGAAGAACCATCGCAGAATGTACAAAGTGATTCCTTCACCATTGGTGAAACTATAGAAGGTGTGGCCAACCAAGAACCAAAGATTAGTAATTCAAGTGAAACTCAACTCACCGAGGAACAGAGAGGTCGAATGGAAGCCAACAGGTTGAAGGCATTAGAAAGAGCTGCAGCCAAGGCTCGCTCTTTGCCCACTGTCTGA

Coding sequence (CDS)

ATGGAGAAGGACAACGCCGCCGTCGCAGCGCCGACGGGATGCTACAAACCTCCTTTCTCCTCTGCTCCTGCTGCCCCTTCAAAAGTTGCAGAGAAGCCCAAGAAAGCCCCCAGAACCAGGCCCAAGCTCACTCCTGAGCTACTCCTTTCTGAGGATGGTCTAGGTTACGTCCTTCGCCATTTTCCTCGCGTTTTCAAGTATCGCGGCCGTGGACACGAGATCAGTGATTTGGGAAACCTTATTGGCTTATACAGGGAATGGCATTCTCATATACTCCCTTATTACTCTTTCGAGCATTTTGTACATAAGGTGGAACAGGTAGCTGCTACAAAACGTGTAAAGATGTGCCTTGGAGAACTAAGGGAGAGGGTTGCCAGGGGAGGGGATCCTACAAAGTGGCGCGAAGCACCCATTTTAAACGATGATGATGTCAATAATGAAAAAGAAGCCAAAGACCCGGAGGGCATATTTGATAATCAGGAAGAGGCTTTGAGGAATAATGGTGCTGATGACATTGAAGAGGACATGCTTCAGGAAGTTTATGAGAAAGCTACGGAAGAACCATCGCAGAATGTACAAAGTGATTCCTTCACCATTGGTGAAACTATAGAAGGTGTGGCCAACCAAGAACCAAAGATTAGTAATTCAAGTGAAACTCAACTCACCGAGGAACAGAGAGGTCGAATGGAAGCCAACAGGTTGAAGGCATTAGAAAGAGCTGCAGCCAAGGCTCGCTCTTTGCCCACTGTCTGA

Protein sequence

MEKDNAAVAAPTGCYKPPFSSAPAAPSKVAEKPKKAPRTRPKLTPELLLSEDGLGYVLRHFPRVFKYRGRGHEISDLGNLIGLYREWHSHILPYYSFEHFVHKVEQVAATKRVKMCLGELRERVARGGDPTKWREAPILNDDDVNNEKEAKDPEGIFDNQEEALRNNGADDIEEDMLQEVYEKATEEPSQNVQSDSFTIGETIEGVANQEPKISNSSETQLTEEQRGRMEANRLKALERAAAKARSLPTV
Homology
BLAST of Bhi06G001544 vs. TAIR 10
Match: AT3G02820.1 (zinc knuckle (CCHC-type) family protein )

HSP 1 Score: 191.8 bits (486), Expect = 6.6e-49
Identity = 131/277 (47.29%), Postives = 166/277 (59.93%), Query Frame = 0

Query: 9   AAPTGCYK---PPF------SSAP----------AAPSKV------------------AE 68
           +APTGC+K   P        SSAP          +APS++                  A 
Sbjct: 3   SAPTGCFKCGRPGHWSRDCPSSAPVAGNNSVSSSSAPSQIPNNEFQRSSSKSGTSIAPAP 62

Query: 69  KPKKAPRTRPKLTPELLLSEDGLGYVLRHFPRVFKYRGRGHEISDLGNLIGLYREWHSHI 128
           K  K    RPKLTPELLLSEDGLGYVLR+FP+ FKYRGRG E+SDLGNLI LY EWH+H+
Sbjct: 63  KVTKTRVQRPKLTPELLLSEDGLGYVLRYFPKSFKYRGRGKEVSDLGNLIRLYSEWHTHL 122

Query: 129 LPYYSFEHFVHKVEQVAATKRVKMCLGELRERVARGGDPTKW---REAPILNDDDVNNEK 188
           LPYYSF+HFVHKV+QVA+TKRVK C+ ELRERVA G DP K    +E   +  DD + ++
Sbjct: 123 LPYYSFDHFVHKVQQVASTKRVKNCINELRERVASGVDPNKLYEKQEENTVPSDDQDMDQ 182

Query: 189 EAKDPEGIFDNQEEALRNNGADDIEEDMLQEVYEKATEEPS--QNVQSDSFTIGETIEGV 244
            + D E I     +A  +  AD  E+ ML E+++ A++ PS  QN+   S    E    +
Sbjct: 183 PSHDEENIPSKSVDA--DTNADAFEDSMLNEIFDNASKLPSDEQNMDKSSELTEEQRARM 242

BLAST of Bhi06G001544 vs. ExPASy Swiss-Prot
Match: Q5F416 (TIMELESS-interacting protein OS=Gallus gallus OX=9031 GN=TIPIN PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.3e-09
Identity = 63/210 (30.00%), Postives = 88/210 (41.90%), Query Frame = 0

Query: 34  KKAPRTRPKLTPELLLSEDGLGYVLRHFPRVFKYRGRGHEISDLGNLIGLYREWHSHILP 93
           K   R+ PKL    L+SE GL   LRH     K++G+GHE  DL  L+     W   + P
Sbjct: 65  KAVKRSIPKLDANRLVSERGLP-ALRHMFDNVKFKGKGHEAEDLKTLLRHMEHWAHRLFP 124

Query: 94  YYSFEHFVHKVEQVAATKRVKMCLGELRERVARGGDPTKWREAPILNDDDVNNEKEAKDP 153
              F+ F+ +VE +   K V+ CL  +R             + PIL++D   NE    + 
Sbjct: 125 KLQFDDFIDRVESLGNKKEVQTCLKRIR------------LDLPILHEDFTANEGGGGES 184

Query: 154 EGIFDNQEEALRNNGADDIEEDMLQEVYEKATEEPSQNVQSDSFTIGETIEGVANQEPKI 213
            G+                         + ATEE    V S S  +GE           +
Sbjct: 185 NGL-------------------------DMATEE----VHSFSGNVGE-----------L 221

Query: 214 SNSSETQLTEEQRGRMEANRLKALERAAAK 244
            +   T LTEEQ+ R++ NR  ALER  AK
Sbjct: 245 DSLPGTTLTEEQQQRIKRNRQLALERRQAK 221

BLAST of Bhi06G001544 vs. ExPASy Swiss-Prot
Match: Q9BVW5 (TIMELESS-interacting protein OS=Homo sapiens OX=9606 GN=TIPIN PE=1 SV=2)

HSP 1 Score: 62.4 bits (150), Expect = 8.4e-09
Identity = 55/184 (29.89%), Postives = 81/184 (44.02%), Query Frame = 0

Query: 16  KPPFSSAPAAPSKVAEKPKKAPRTRPKLTPELLLSEDGLGYVLRHFPRVFKYRGRGHEIS 75
           +P   S   AP +V  K +   R  PKL  + L+SE GL   LRH     K++G+GHE  
Sbjct: 41  EPDEESGNGAPVRVPPK-RTVKRNIPKLDAQRLISERGLP-ALRHVFDKAKFKGKGHEAE 100

Query: 76  DLGNLIGLYREWHSHILPYYSFEHFVHKVEQVAATKRVKMCLGELRERVARGGDPTKWRE 135
           DL  LI     W   + P   FE F+ +VE + + K V+ CL  +R             +
Sbjct: 101 DLKMLIRHMEHWAHRLFPKLQFEDFIDRVEYLGSKKEVQTCLKRIR------------LD 160

Query: 136 APILNDDDVNNEKEAKDPEGIFDNQEEALRNNGADDI------EEDMLQEVYEKATEEPS 194
            PIL++D V+N  E      + +N E  + +   D         E    E+    TEE  
Sbjct: 161 LPILHEDFVSNNDE------VAENNEHDVTSTELDPFLTNLSESEMFASELSRSLTEEQQ 204

BLAST of Bhi06G001544 vs. ExPASy Swiss-Prot
Match: Q3ZCC4 (TIMELESS-interacting protein OS=Bos taurus OX=9913 GN=TIPIN PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.9e-08
Identity = 64/231 (27.71%), Postives = 97/231 (41.99%), Query Frame = 0

Query: 16  KPPFSSAPAAPSKVAEKPKKAPRTRPKLTPELLLSEDGLGYVLRHFPRVFKYRGRGHEIS 75
           +P   S   AP  V  K +   R  PKL  E L+SE GL   LRH     K++G+GHE  
Sbjct: 41  EPEEESGRGAPVPVPPK-RTVKRNIPKLNAERLISERGLP-ALRHVFEKAKFKGKGHEAE 100

Query: 76  DLGNLIGLYREWHSHILPYYSFEHFVHKVEQVAATKRVKMCLGELRERVARGGDPTKWRE 135
           DL  LI     W   + P   FE F+ +VE +   K V+ CL  +R             +
Sbjct: 101 DLKTLIRHMEHWAHRLFPKLQFEDFIDRVECLGNKKEVQTCLKRIR------------LD 160

Query: 136 APILNDDDVNNEKEAKDPEG--IFDNQEEALRNNGADDIEEDMLQEVYEKATEEPSQNVQ 195
            PIL++D V+N  E ++  G  +   + +    N    +E     E     TEE  Q ++
Sbjct: 161 LPILHEDFVSNNDEVEENNGHDVTATELDHFLTNSYGSVE--FASESSRSLTEEEQQRIE 220

Query: 196 ---------------SDSFTIGE--TIEGVANQEPKISNSSETQLTEEQRG 228
                          S+S ++G   ++   + Q  +  ++ E Q  EE  G
Sbjct: 221 RNKQLALERRQAKLLSNSQSLGNDLSVNTPSTQTSEAGSTGEEQKEEESNG 255

BLAST of Bhi06G001544 vs. ExPASy Swiss-Prot
Match: Q91WA1 (TIMELESS-interacting protein OS=Mus musculus OX=10090 GN=Tipin PE=1 SV=2)

HSP 1 Score: 58.9 bits (141), Expect = 9.3e-08
Identity = 70/239 (29.29%), Postives = 94/239 (39.33%), Query Frame = 0

Query: 11  PTGCYKPPFSSAP--AAPSKVAEKPKK-APRTRPKLTPELLLSEDGLGYVLRHFPRVFKY 70
           P    + P  + P   + S V   PK+   R  PKL    L SE GL   LRH     K+
Sbjct: 29  PASPERDPADAEPEEGSGSGVPVPPKRTVKRNLPKLDATRLTSERGLP-ALRHVFDKTKF 88

Query: 71  RGRGHEISDLGNLIGLYREWHSHILPYYSFEHFVHKVEQVAATKRVKMCLGELRERVARG 130
           +G+GHE  DL  LI     W   + P   FE F+ +VE +   K V+ CL  +R      
Sbjct: 89  KGKGHEAEDLKTLIRHMEHWAHRLFPKLQFEDFIDRVENLGNKKEVQTCLKRIR------ 148

Query: 131 GDPTKWREAPILNDDDVNNEKEAKDPEGIFDNQEEALRNNGADDIEEDMLQEVYEKATEE 190
                  + PI+++D VNN  E  +              NG D                +
Sbjct: 149 ------LDLPIVHEDFVNNNDEVGEA-------------NGPD----------VSATGFD 208

Query: 191 PSQNVQSDSFTIGETIEGVANQEPKISNSSETQLTEEQRGRMEANRLKALERAAAKARS 247
           P     SDS               K ++     LTEEQ+ R+E N+  ALER  AK  S
Sbjct: 209 PFLTSSSDS--------------RKFASEPTRSLTEEQQQRIERNKQLALERRQAKLLS 217

BLAST of Bhi06G001544 vs. ExPASy Swiss-Prot
Match: Q4QR88 (TIMELESS-interacting protein OS=Rattus norvegicus OX=10116 GN=Tipin PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 5.1e-06
Identity = 61/243 (25.10%), Postives = 101/243 (41.56%), Query Frame = 0

Query: 2   EKDNAAVAAPTGCYKPPFSSAPAAPSKVAEKPKKAPRTRPKLTPELLLSEDGLGYVLRHF 61
           E+D A      G   P     P  P ++ +      R  PKL    L SE GL   LRH 
Sbjct: 33  ERDPAEAEPDEGSGAP----VPVPPKRIVK------RNIPKLDATRLTSERGLP-ALRHV 92

Query: 62  PRVFKYRGRGHEISDLGNLIGLYREWHSHILPYYSFEHFVHKVEQVAATKRVKMCLGELR 121
               K++G+GHE  DL  LI     W   + P   FE F+ +VE +   K V+ CL  +R
Sbjct: 93  FDKTKFKGKGHEAEDLKTLIRHMEHWAHRLFPKLQFEDFIDRVENLGNKKEVQTCLKRIR 152

Query: 122 ERVARGGDPTKWREAPILNDDDVNNEKEAKDPEGI------FD------NQEEALRNNGA 181
                        + PI+++D VNN  E ++   +      FD      +  +   +  +
Sbjct: 153 ------------LDLPIVHEDFVNNNDEVEETNSLDAAATGFDAFVTSSSDSKRFASEAS 212

Query: 182 DDIEEDMLQEVYEKATEEPSQNVQSDSFTIGETIEGVANQEPKISNSSETQLTEEQRGRM 233
            ++ E+  Q + EK  +   +  Q+   +  +++E     +  +  SS  +  EE  G +
Sbjct: 213 RNLTEEQQQRI-EKNKQLALERRQAKLLSNSQSLE----NDVTVEESSTGENQEESNGLI 247

BLAST of Bhi06G001544 vs. ExPASy TrEMBL
Match: A0A5D3BGW4 (TIMELESS-interacting protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001060 PE=3 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 8.5e-113
Identity = 231/283 (81.63%), Postives = 236/283 (83.39%), Query Frame = 0

Query: 1   MEKDNAAVAAPTGCY--------------------------KPPFSSAPAAPSKVAEKPK 60
           M+KDN A+AAPTGCY                          KPPFSSAPAAPSK  EKPK
Sbjct: 1   MDKDNNAIAAPTGCYKCGKPGHWSRDCPSSAPNPNPTSNSSKPPFSSAPAAPSKAVEKPK 60

Query: 61  KAPRTRPKLTPELLLSEDGLGYVLRHFPRVFKYRGRGHEISDLGNLIGLYREWHSHILPY 120
           KAPRTRPKLTPELLLSEDGLGYVLRHFPR FKYRGRGHEISDL NLIGLYREWHSHILPY
Sbjct: 61  KAPRTRPKLTPELLLSEDGLGYVLRHFPRAFKYRGRGHEISDLENLIGLYREWHSHILPY 120

Query: 121 YSFEHFVHKVEQVAATKRVKMCLGELRERVARGGDPTKWREAPILN-DDDVNNEKEAKDP 180
           YSFEHFVHKVEQVAATKRVKMCLGELRERVARGGDPTKWREAPILN DDDVNNEKEAKDP
Sbjct: 121 YSFEHFVHKVEQVAATKRVKMCLGELRERVARGGDPTKWREAPILNDDDDVNNEKEAKDP 180

Query: 181 EGIFDNQEEA-LRNNGADDIEEDMLQEVYEKATEEPSQNVQSDSFTIGETIEGVA----N 240
           EG+FDN EEA LRNNGADDIEEDMLQEVYEKATEEP QN QSDS T G T+EGVA    N
Sbjct: 181 EGVFDNPEEASLRNNGADDIEEDMLQEVYEKATEEPLQNEQSDSLTTGGTMEGVANQEPN 240

Query: 241 QEPKISNSSET-QLTEEQRGRMEANRLKALERAAAKARSLPTV 251
           QEPK SNSSET +LTEEQR RMEANRLKALERAAAKARSL TV
Sbjct: 241 QEPKTSNSSETSELTEEQRVRMEANRLKALERAAAKARSLATV 283

BLAST of Bhi06G001544 vs. ExPASy TrEMBL
Match: A0A1S3C4P4 (TIMELESS-interacting protein OS=Cucumis melo OX=3656 GN=LOC103496437 PE=3 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 8.5e-113
Identity = 231/283 (81.63%), Postives = 236/283 (83.39%), Query Frame = 0

Query: 1   MEKDNAAVAAPTGCY--------------------------KPPFSSAPAAPSKVAEKPK 60
           M+KDN A+AAPTGCY                          KPPFSSAPAAPSK  EKPK
Sbjct: 1   MDKDNNAIAAPTGCYKCGKPGHWSRDCPSSAPNPNPTSNSSKPPFSSAPAAPSKAVEKPK 60

Query: 61  KAPRTRPKLTPELLLSEDGLGYVLRHFPRVFKYRGRGHEISDLGNLIGLYREWHSHILPY 120
           KAPRTRPKLTPELLLSEDGLGYVLRHFPR FKYRGRGHEISDL NLIGLYREWHSHILPY
Sbjct: 61  KAPRTRPKLTPELLLSEDGLGYVLRHFPRAFKYRGRGHEISDLENLIGLYREWHSHILPY 120

Query: 121 YSFEHFVHKVEQVAATKRVKMCLGELRERVARGGDPTKWREAPILN-DDDVNNEKEAKDP 180
           YSFEHFVHKVEQVAATKRVKMCLGELRERVARGGDPTKWREAPILN DDDVNNEKEAKDP
Sbjct: 121 YSFEHFVHKVEQVAATKRVKMCLGELRERVARGGDPTKWREAPILNDDDDVNNEKEAKDP 180

Query: 181 EGIFDNQEEA-LRNNGADDIEEDMLQEVYEKATEEPSQNVQSDSFTIGETIEGVA----N 240
           EG+FDN EEA LRNNGADDIEEDMLQEVYEKATEEP QN QSDS T G T+EGVA    N
Sbjct: 181 EGVFDNPEEASLRNNGADDIEEDMLQEVYEKATEEPLQNEQSDSLTTGGTMEGVANQEPN 240

Query: 241 QEPKISNSSET-QLTEEQRGRMEANRLKALERAAAKARSLPTV 251
           QEPK SNSSET +LTEEQR RMEANRLKALERAAAKARSL TV
Sbjct: 241 QEPKTSNSSETSELTEEQRVRMEANRLKALERAAAKARSLATV 283

BLAST of Bhi06G001544 vs. ExPASy TrEMBL
Match: A0A0A0LI96 (CCHC-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009510 PE=3 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 1.1e-112
Identity = 228/280 (81.43%), Postives = 235/280 (83.93%), Query Frame = 0

Query: 1   MEKDNAAVAAPTGCYK--------------------------PPFSSAPAAPSKVAEKPK 60
           MEKDN AV APTGC+K                          P FSS PAAPSK  EKPK
Sbjct: 1   MEKDNNAVPAPTGCFKCGKPGHWSRDCPSSAPNPNPTSNSSTPSFSSPPAAPSKAVEKPK 60

Query: 61  KAPRTRPKLTPELLLSEDGLGYVLRHFPRVFKYRGRGHEISDLGNLIGLYREWHSHILPY 120
           KAPRTRPKLTPELLLS+DGLGYVLRHFPR FKYRGRGHEI+DL NLIGLYREWHSHILPY
Sbjct: 61  KAPRTRPKLTPELLLSDDGLGYVLRHFPRAFKYRGRGHEINDLENLIGLYREWHSHILPY 120

Query: 121 YSFEHFVHKVEQVAATKRVKMCLGELRERVARGGDPTKWREAPILN--DDDVNNEKEAKD 180
           YSFEHFVHKVEQVAATKRVKMCLGELRERVARGGDPTKWREAPILN  DDDVNNEKEAKD
Sbjct: 121 YSFEHFVHKVEQVAATKRVKMCLGELRERVARGGDPTKWREAPILNDDDDDVNNEKEAKD 180

Query: 181 PEGIFDNQEEA-LRNNGADDIEEDMLQEVYEKATEEPSQNVQSDSFTIGETIEGVANQEP 240
           P+ +FDNQEEA LRNNGADDIEEDMLQEVYEKATEEP QN QSDS TIG T+EGVANQEP
Sbjct: 181 PDDVFDNQEEASLRNNGADDIEEDMLQEVYEKATEEPLQNEQSDSLTIGGTMEGVANQEP 240

Query: 241 KISNSSET-QLTEEQRGRMEANRLKALERAAAKARSLPTV 251
           K SNSSET +LTEEQR RMEANRLKALERAAAKARSLPTV
Sbjct: 241 KTSNSSETSELTEEQRVRMEANRLKALERAAAKARSLPTV 280

BLAST of Bhi06G001544 vs. ExPASy TrEMBL
Match: A0A6J1KG39 (TIMELESS-interacting protein OS=Cucurbita maxima OX=3661 GN=LOC111493575 PE=3 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 4.0e-110
Identity = 222/276 (80.43%), Postives = 233/276 (84.42%), Query Frame = 0

Query: 1   MEKDNAAVAAPTGCYK----------------------PPFSSAPAAPSKVAEKPKKAPR 60
           M+KDNAAVAAPTGCYK                      PP SSAPAAP KVAEKPKKAPR
Sbjct: 1   MDKDNAAVAAPTGCYKCGKPGHWSRDCPSSAPNPNFSRPPSSSAPAAPPKVAEKPKKAPR 60

Query: 61  TRPKLTPELLLSEDGLGYVLRHFPRVFKYRGRGHEISDLGNLIGLYREWHSHILPYYSFE 120
            RPKLTPELLLSEDGLGYVLRHFPR FKYRGRGHEISDL NLIGLYREWHSHILPYY FE
Sbjct: 61  VRPKLTPELLLSEDGLGYVLRHFPRAFKYRGRGHEISDLENLIGLYREWHSHILPYYPFE 120

Query: 121 HFVHKVEQVAATKRVKMCLGELRERVARGGDPTKWREAPILN--DDDVNNEKEAKDPEGI 180
           HFVHKVEQVAATKRVKMCLGELRE+VARGGDPTKWRE+PI+N  DDD+NNE EAKD EG+
Sbjct: 121 HFVHKVEQVAATKRVKMCLGELREKVARGGDPTKWRESPIVNDADDDINNENEAKDSEGL 180

Query: 181 FDNQEEA-LRNNGADDIEEDMLQEVYEKAT-EEPSQNVQSDSFTIGETIEGVANQ-EPKI 240
           FDNQEEA L+NNGADDIEEDMLQEVYEKAT EEP  N+QSDSFTIGET+EGV NQ EP  
Sbjct: 181 FDNQEEASLKNNGADDIEEDMLQEVYEKATEEEPLMNLQSDSFTIGETVEGVVNQEEPNT 240

Query: 241 SNSSETQLTEEQRGRMEANRLKALERAAAKARSLPT 250
           SNS+ET LTE+QR RMEANRLKALERAAAKARS PT
Sbjct: 241 SNSNETLLTEQQRARMEANRLKALERAAAKARSSPT 276

BLAST of Bhi06G001544 vs. ExPASy TrEMBL
Match: A0A6J1G4B9 (TIMELESS-interacting protein OS=Cucurbita moschata OX=3662 GN=LOC111450673 PE=3 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 5.2e-110
Identity = 222/276 (80.43%), Postives = 233/276 (84.42%), Query Frame = 0

Query: 1   MEKDNAAVAAPTGCYK----------------------PPFSSAPAAPSKVAEKPKKAPR 60
           M+KDNAAVAAPTGCYK                      PP SSAPAAPSKVAEKPKKAPR
Sbjct: 1   MDKDNAAVAAPTGCYKCGKPGHWSRDCPSSAPNPNSSSPPSSSAPAAPSKVAEKPKKAPR 60

Query: 61  TRPKLTPELLLSEDGLGYVLRHFPRVFKYRGRGHEISDLGNLIGLYREWHSHILPYYSFE 120
            RPKLTPELLLSEDGLGYVLRHFPR FKYRGRGHEISDL NLIGLYREWHSHILPYY FE
Sbjct: 61  VRPKLTPELLLSEDGLGYVLRHFPRAFKYRGRGHEISDLENLIGLYREWHSHILPYYPFE 120

Query: 121 HFVHKVEQVAATKRVKMCLGELRERVARGGDPTKWREAPILN--DDDVNNEKEAKDPEGI 180
           HFVHKVEQVAATKRVKMCLGELRE+VARGGDPTKWRE+PI+N  DDD+NNE EAKD EG+
Sbjct: 121 HFVHKVEQVAATKRVKMCLGELREKVARGGDPTKWRESPIVNDADDDINNENEAKDSEGL 180

Query: 181 FDNQEEA-LRNNGADDIEEDMLQEVYEKAT-EEPSQNVQSDSFTIGETIEGVANQ-EPKI 240
           FDNQEEA  +NNGADDIEEDMLQEVYEKAT EEP  N+QSDSFTIGET+EGV NQ EP  
Sbjct: 181 FDNQEEASFKNNGADDIEEDMLQEVYEKATEEEPLMNLQSDSFTIGETMEGVVNQEEPNT 240

Query: 241 SNSSETQLTEEQRGRMEANRLKALERAAAKARSLPT 250
           SNS+ET LTE+QR RMEANRLKALERAAAKARS PT
Sbjct: 241 SNSNETLLTEQQRARMEANRLKALERAAAKARSSPT 276

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G02820.16.6e-4947.29zinc knuckle (CCHC-type) family protein [more]
Match NameE-valueIdentityDescription
Q5F4161.3e-0930.00TIMELESS-interacting protein OS=Gallus gallus OX=9031 GN=TIPIN PE=2 SV=1[more]
Q9BVW58.4e-0929.89TIMELESS-interacting protein OS=Homo sapiens OX=9606 GN=TIPIN PE=1 SV=2[more]
Q3ZCC41.9e-0827.71TIMELESS-interacting protein OS=Bos taurus OX=9913 GN=TIPIN PE=2 SV=1[more]
Q91WA19.3e-0829.29TIMELESS-interacting protein OS=Mus musculus OX=10090 GN=Tipin PE=1 SV=2[more]
Q4QR885.1e-0625.10TIMELESS-interacting protein OS=Rattus norvegicus OX=10116 GN=Tipin PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BGW48.5e-11381.63TIMELESS-interacting protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3C4P48.5e-11381.63TIMELESS-interacting protein OS=Cucumis melo OX=3656 GN=LOC103496437 PE=3 SV=1[more]
A0A0A0LI961.1e-11281.43CCHC-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009510 P... [more]
A0A6J1KG394.0e-11080.43TIMELESS-interacting protein OS=Cucurbita maxima OX=3661 GN=LOC111493575 PE=3 SV... [more]
A0A6J1G4B95.2e-11080.43TIMELESS-interacting protein OS=Cucurbita moschata OX=3662 GN=LOC111450673 PE=3 ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012923Chromosome segregation in meiosis protein 3PFAMPF07962Swi3coord: 42..123
e-value: 1.5E-23
score: 82.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..42
NoneNo IPR availablePANTHERPTHR13220:SF11TIMELESS-INTERACTING PROTEINcoord: 21..245
IPR040038TIPIN/Csm3/Swi3PANTHERPTHR13220TIMELESS INTERACTING-RELATEDcoord: 21..245

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi06M001544Bhi06M001544mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006974 cellular response to DNA damage stimulus
biological_process GO:0000076 DNA replication checkpoint signaling
biological_process GO:0043111 replication fork arrest
biological_process GO:0048478 replication fork protection
cellular_component GO:0031298 replication fork protection complex
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0008270 zinc ion binding