Bhi06G001397 (gene) Wax gourd (B227) v1

Overview
NameBhi06G001397
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionThreonine synthase
Locationchr6: 46462443 .. 46464038 (+)
RNA-Seq ExpressionBhi06G001397
SyntenyBhi06G001397
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTCCTCCCTTTTCTCTTTCAACACCTCTTTCTCTACCAAACCCGATTCTCTCTTCCACCGCTCCGCTCGCCGGAACTCTTCCTCTCCTTTCACCGTCGTCTGTTCCTCCGCCACTTCCGACTCTGCCGCCTCCCCTGTTCTCCACTCATCTCCGCCCCTCGGACACCAGTTCGCTTCTGCCAATATTCAGGACGATTCTCATCGCCGTTGTTCCGTAGCCGTTGACGTCGGCCATAATTTCTCGGCGAAGTATGTGCCGTTTGGTTCTGGCTATGATTCCTCCGAGTGGTACTCGCTCGACGAAATTGTTTATCGGAGCCGGTCCGGCGGGCTTCTCGATGTTCAGCACAACATGGAAGCGTTGAAGAAATTCGACGGAGCGTATTGGCGAAATCTGTTCGATTCGCGCGTGGGGAAGACGACCTGGCCGTACGGGTCCGGCGTTTGGAGCAAGAAAGAGTGGGTACTGCCGGAGATTGATTCCAGCGACATCGTCAGCGCCTTCGAAGGAAATTCGAATCTCTTCTGGGCGGAGCGTTTTGGGAAACAGTTTCTAGGTATGAATGATCTATGGGTCAAACATTGCGGGATTAGCCACACCGGTAGTTTCAAAGACCTTGGAATGACTGTTTTGGTCAGCCAAGTCAATCGCCTCCGGAAAATGAACCGCCCTGTCTTCGGCGTCGGCTGCGCCTCAACTGGAGACACCTCCGCCGCTCTCTCCGCCTACTGCGCTGCCGCAGGAATTCCATCCATTGTCTTCCTCCCAGCCGATAAAATTTCCATCGCGCAATTAGTTCAGCCAATTGCAAACGGCGCTTTCGTCTTAAGCATGGATACCGACTTCGACGGCTGTATGAAACTAATCCGTGAAGTCACGGCGGAGCTTCCGATTTACCTGGCGAATTCTCTCAACAGTTTGAGACTGGAAGGGCAAAAGACGGCGGCCATTGAAATCCTTCAACAGTTCGACTGGGAAGTGCCAGATTGGGTAATCATACCAGGCGGAAACCTAGGGAATATATATGCATTTTACAAAGGCTTTCAAATGTGCAAAGAATTAGGTCTCGTCGACAAGATTCCACGCCTGGTTTGTGCTCAAGCGGCAAATGCAAATCCGCTTTACTTGTATTACAAATCTGGTTGGAAAGAGTTCTCGCCATTGAAGGCGACCACGACTTTTGCTTCTGCAATACAGATTGGGGATCCAGTTTCCATTGACAGAGCTGTGTTCGCTCTGAAAAACTCCGGCGGAATTGTGGAAGAAGCGACGGAAGAGGAACTAATGGACGCCACGGCTCAGGCTGATTCGACGGGGATGTTCATTTGCCCTCACACAGGGGTTGCATTAACAGCTCTGATTAAGCTGAGAAACCGCGGCGTCATCGGAGCGAGTGACCGGACGGTGGTGGTAAGCACTGCTCATGGTCTGAAATTCACTCAATCCAAGATCGATTACCACTCGCGAGCAATCCCGGGGATGGAATGCCGATATGCAAACCCGCCGGTGGAAGTGAAAGCGGAAGTTGGGGCTGTTATGGATGTTCTTAAGGAACATCTGTTGAAGAAGACTCCTAAATTTGAGTCATAG

mRNA sequence

ATGGCCTCCTCCCTTTTCTCTTTCAACACCTCTTTCTCTACCAAACCCGATTCTCTCTTCCACCGCTCCGCTCGCCGGAACTCTTCCTCTCCTTTCACCGTCGTCTGTTCCTCCGCCACTTCCGACTCTGCCGCCTCCCCTGTTCTCCACTCATCTCCGCCCCTCGGACACCAGTTCGCTTCTGCCAATATTCAGGACGATTCTCATCGCCGTTGTTCCGTAGCCGTTGACGTCGGCCATAATTTCTCGGCGAAGTATGTGCCGTTTGGTTCTGGCTATGATTCCTCCGAGTGGTACTCGCTCGACGAAATTGTTTATCGGAGCCGGTCCGGCGGGCTTCTCGATGTTCAGCACAACATGGAAGCGTTGAAGAAATTCGACGGAGCGTATTGGCGAAATCTGTTCGATTCGCGCGTGGGGAAGACGACCTGGCCGTACGGGTCCGGCGTTTGGAGCAAGAAAGAGTGGGTACTGCCGGAGATTGATTCCAGCGACATCGTCAGCGCCTTCGAAGGAAATTCGAATCTCTTCTGGGCGGAGCGTTTTGGGAAACAGTTTCTAGGTATGAATGATCTATGGGTCAAACATTGCGGGATTAGCCACACCGGTAGTTTCAAAGACCTTGGAATGACTGTTTTGGTCAGCCAAGTCAATCGCCTCCGGAAAATGAACCGCCCTGTCTTCGGCGTCGGCTGCGCCTCAACTGGAGACACCTCCGCCGCTCTCTCCGCCTACTGCGCTGCCGCAGGAATTCCATCCATTGTCTTCCTCCCAGCCGATAAAATTTCCATCGCGCAATTAGTTCAGCCAATTGCAAACGGCGCTTTCGTCTTAAGCATGGATACCGACTTCGACGGCTGTATGAAACTAATCCGTGAAGTCACGGCGGAGCTTCCGATTTACCTGGCGAATTCTCTCAACAGTTTGAGACTGGAAGGGCAAAAGACGGCGGCCATTGAAATCCTTCAACAGTTCGACTGGGAAGTGCCAGATTGGGTAATCATACCAGGCGGAAACCTAGGGAATATATATGCATTTTACAAAGGCTTTCAAATGTGCAAAGAATTAGGTCTCGTCGACAAGATTCCACGCCTGGTTTGTGCTCAAGCGGCAAATGCAAATCCGCTTTACTTGTATTACAAATCTGGTTGGAAAGAGTTCTCGCCATTGAAGGCGACCACGACTTTTGCTTCTGCAATACAGATTGGGGATCCAGTTTCCATTGACAGAGCTGTGTTCGCTCTGAAAAACTCCGGCGGAATTGTGGAAGAAGCGACGGAAGAGGAACTAATGGACGCCACGGCTCAGGCTGATTCGACGGGGATGTTCATTTGCCCTCACACAGGGGTTGCATTAACAGCTCTGATTAAGCTGAGAAACCGCGGCGTCATCGGAGCGAGTGACCGGACGGTGGTGGTAAGCACTGCTCATGGTCTGAAATTCACTCAATCCAAGATCGATTACCACTCGCGAGCAATCCCGGGGATGGAATGCCGATATGCAAACCCGCCGGTGGAAGTGAAAGCGGAAGTTGGGGCTGTTATGGATGTTCTTAAGGAACATCTGTTGAAGAAGACTCCTAAATTTGAGTCATAG

Coding sequence (CDS)

ATGGCCTCCTCCCTTTTCTCTTTCAACACCTCTTTCTCTACCAAACCCGATTCTCTCTTCCACCGCTCCGCTCGCCGGAACTCTTCCTCTCCTTTCACCGTCGTCTGTTCCTCCGCCACTTCCGACTCTGCCGCCTCCCCTGTTCTCCACTCATCTCCGCCCCTCGGACACCAGTTCGCTTCTGCCAATATTCAGGACGATTCTCATCGCCGTTGTTCCGTAGCCGTTGACGTCGGCCATAATTTCTCGGCGAAGTATGTGCCGTTTGGTTCTGGCTATGATTCCTCCGAGTGGTACTCGCTCGACGAAATTGTTTATCGGAGCCGGTCCGGCGGGCTTCTCGATGTTCAGCACAACATGGAAGCGTTGAAGAAATTCGACGGAGCGTATTGGCGAAATCTGTTCGATTCGCGCGTGGGGAAGACGACCTGGCCGTACGGGTCCGGCGTTTGGAGCAAGAAAGAGTGGGTACTGCCGGAGATTGATTCCAGCGACATCGTCAGCGCCTTCGAAGGAAATTCGAATCTCTTCTGGGCGGAGCGTTTTGGGAAACAGTTTCTAGGTATGAATGATCTATGGGTCAAACATTGCGGGATTAGCCACACCGGTAGTTTCAAAGACCTTGGAATGACTGTTTTGGTCAGCCAAGTCAATCGCCTCCGGAAAATGAACCGCCCTGTCTTCGGCGTCGGCTGCGCCTCAACTGGAGACACCTCCGCCGCTCTCTCCGCCTACTGCGCTGCCGCAGGAATTCCATCCATTGTCTTCCTCCCAGCCGATAAAATTTCCATCGCGCAATTAGTTCAGCCAATTGCAAACGGCGCTTTCGTCTTAAGCATGGATACCGACTTCGACGGCTGTATGAAACTAATCCGTGAAGTCACGGCGGAGCTTCCGATTTACCTGGCGAATTCTCTCAACAGTTTGAGACTGGAAGGGCAAAAGACGGCGGCCATTGAAATCCTTCAACAGTTCGACTGGGAAGTGCCAGATTGGGTAATCATACCAGGCGGAAACCTAGGGAATATATATGCATTTTACAAAGGCTTTCAAATGTGCAAAGAATTAGGTCTCGTCGACAAGATTCCACGCCTGGTTTGTGCTCAAGCGGCAAATGCAAATCCGCTTTACTTGTATTACAAATCTGGTTGGAAAGAGTTCTCGCCATTGAAGGCGACCACGACTTTTGCTTCTGCAATACAGATTGGGGATCCAGTTTCCATTGACAGAGCTGTGTTCGCTCTGAAAAACTCCGGCGGAATTGTGGAAGAAGCGACGGAAGAGGAACTAATGGACGCCACGGCTCAGGCTGATTCGACGGGGATGTTCATTTGCCCTCACACAGGGGTTGCATTAACAGCTCTGATTAAGCTGAGAAACCGCGGCGTCATCGGAGCGAGTGACCGGACGGTGGTGGTAAGCACTGCTCATGGTCTGAAATTCACTCAATCCAAGATCGATTACCACTCGCGAGCAATCCCGGGGATGGAATGCCGATATGCAAACCCGCCGGTGGAAGTGAAAGCGGAAGTTGGGGCTGTTATGGATGTTCTTAAGGAACATCTGTTGAAGAAGACTCCTAAATTTGAGTCATAG

Protein sequence

MASSLFSFNTSFSTKPDSLFHRSARRNSSSPFTVVCSSATSDSAASPVLHSSPPLGHQFASANIQDDSHRRCSVAVDVGHNFSAKYVPFGSGYDSSEWYSLDEIVYRSRSGGLLDVQHNMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVFGVGCASTGDTSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELGLVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALKNSGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIGASDRTVVVSTAHGLKFTQSKIDYHSRAIPGMECRYANPPVEVKAEVGAVMDVLKEHLLKKTPKFES
Homology
BLAST of Bhi06G001397 vs. TAIR 10
Match: AT4G29840.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 813.9 bits (2101), Expect = 7.5e-236
Identity = 415/530 (78.30%), Postives = 457/530 (86.23%), Query Frame = 0

Query: 1   MASSLFSFNTSFST---KPDSL-FHRSARRNSSSPFTVVCSSATSDSAASPVLHSSPPLG 60
           MASS   FN S S+   K D +  HRS       P  + C++  ++  A       PP  
Sbjct: 1   MASSCL-FNASVSSLNPKQDPIRRHRSTSLLRHRPVVISCTADGNNIKAPIETAVKPP-- 60

Query: 61  HQFASANIQDDSHRRCSVAVDVGHNFSAKYVPFGSGYDSSEWYSLDEIVYRSRSGGLLDV 120
           H+    NI+D++ R  S AV   + FSAKYVPF +   S+E YSLDEIVYRSRSGGLLDV
Sbjct: 61  HR-TEDNIRDEARRNRSNAV---NPFSAKYVPFNAAPGSTESYSLDEIVYRSRSGGLLDV 120

Query: 121 QHNMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNL 180
           +H+MEALK+FDGAYWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEID  DIVSAFEGNSNL
Sbjct: 121 EHDMEALKRFDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNL 180

Query: 181 FWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVFGVGCASTG 240
           FWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKM RPV GVGCASTG
Sbjct: 181 FWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCASTG 240

Query: 241 DTSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTA 300
           DTSAALSAYCA+AGIPSIVFLPA+KIS+AQLVQPIANGAFVLS+DTDFDGCMKLIRE+TA
Sbjct: 241 DTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREITA 300

Query: 301 ELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKEL 360
           ELPIYLANSLNSLRLEGQKTAAIEILQQFDW+VPDWVI+PGGNLGNIYAFYKGF+MC+EL
Sbjct: 301 ELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQEL 360

Query: 361 GLVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALK 420
           GLVD+IPR+VCAQAANANPLYL+YKSGWK+F P+ A+TTFASAIQIGDPVSIDRAV+ALK
Sbjct: 361 GLVDRIPRMVCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYALK 420

Query: 421 NSGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIGASDRTVVVSTA 480
              GIVEEATEEELMDA AQADSTGMFICPHTGVALTAL KLRN+GVI  +DRTVVVSTA
Sbjct: 421 KCNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVSTA 480

Query: 481 HGLKFTQSKIDYHSRAIPGMECRYANPPVEVKAEVGAVMDVLKEHLLKKT 527
           HGLKFTQSKIDYHS AIP M CR++NPPV+VKA+ GAVMDVLK +L   T
Sbjct: 481 HGLKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYLGSNT 523

BLAST of Bhi06G001397 vs. TAIR 10
Match: AT1G72810.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 793.1 bits (2047), Expect = 1.4e-229
Identity = 401/501 (80.04%), Postives = 440/501 (87.82%), Query Frame = 0

Query: 23  SARRNSSSPFTVVCSSATSDSAASPVLHSSPPLGHQFASANIQDDSHRRCSVAVDVGHNF 82
           S + +S++ FTV C+SA   S A P    +P    +    NI+D++ RR         N 
Sbjct: 20  SLKPHSAASFTVRCTSA---SPAVP--PQTPQKPRRSPDENIRDEARRRPHQL----QNL 79

Query: 83  SAKYVPFGSGYDSSEWYSLDEIVYRSRSGGLLDVQHNMEALKKFDGAYWRNLFDSRVGKT 142
           SA+YVPF +   S+E YSLDEIVYRS+SG LLDVQH+  ALK++DG +WRNLFDSRVGKT
Sbjct: 80  SARYVPFNAPPSSTESYSLDEIVYRSQSGALLDVQHDFAALKRYDGEFWRNLFDSRVGKT 139

Query: 143 TWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHT 202
            WPYGSGVWSKKEWVLPEID  DIVSAFEGNSNLFWAERFGKQ+L MNDLWVKHCGISHT
Sbjct: 140 NWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFWAERFGKQYLQMNDLWVKHCGISHT 199

Query: 203 GSFKDLGMTVLVSQVNRLRKMNRPVFGVGCASTGDTSAALSAYCAAAGIPSIVFLPADKI 262
           GSFKDLGM+VLVSQVNRLRKMN+PV GVGCASTGDTSAALSAYCA+AGIPSIVFLPADKI
Sbjct: 200 GSFKDLGMSVLVSQVNRLRKMNKPVIGVGCASTGDTSAALSAYCASAGIPSIVFLPADKI 259

Query: 263 SIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEIL 322
           S+AQLVQPIANGAFVLS+DTDFDGCM LIREVTAELPIYLANSLNSLRLEGQKTAAIEIL
Sbjct: 260 SMAQLVQPIANGAFVLSIDTDFDGCMHLIREVTAELPIYLANSLNSLRLEGQKTAAIEIL 319

Query: 323 QQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELGLVDKIPRLVCAQAANANPLYLYYKS 382
           QQF+W+VPDWVI+PGGNLGNIYAFYKGF MCKELGLVD+IPRLVCAQAANANPLYL+YKS
Sbjct: 320 QQFNWQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDRIPRLVCAQAANANPLYLHYKS 379

Query: 383 GWKE-FSPLKATTTFASAIQIGDPVSIDRAVFALKNSGGIVEEATEEELMDATAQADSTG 442
           G+KE F+PLKA TTFASAIQIGDPVSIDRAV+ALK S GIVEEATEEELMDATA ADSTG
Sbjct: 380 GFKEDFNPLKANTTFASAIQIGDPVSIDRAVYALKKSNGIVEEATEEELMDATALADSTG 439

Query: 443 MFICPHTGVALTALIKLRNRGVIGASDRTVVVSTAHGLKFTQSKIDYHSRAIPGMECRYA 502
           MFICPHTGVALTAL+KLR  GVIGA+DRTVVVSTAHGLKFTQSKIDYHS+ I  M CR A
Sbjct: 440 MFICPHTGVALTALMKLRKSGVIGANDRTVVVSTAHGLKFTQSKIDYHSKNIKEMACRLA 499

Query: 503 NPPVEVKAEVGAVMDVLKEHL 523
           NPPV+VKA+ G+VMDVLKE+L
Sbjct: 500 NPPVKVKAKFGSVMDVLKEYL 511

BLAST of Bhi06G001397 vs. ExPASy Swiss-Prot
Match: Q9MT28 (Threonine synthase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 9.8e-241
Identity = 420/530 (79.25%), Postives = 460/530 (86.79%), Query Frame = 0

Query: 1   MASSLFSFNTSFSTKPDSLFHRSARR--NSSSPFTVVCSSATSDSAASPVLHSSPPLGHQ 60
           MA+S    ++  S     L H+S  +  N    FT + ++AT+D A S   H  P     
Sbjct: 1   MAASCMLRSSFISPGLPQLHHQSTSKPNNGIHFFTPIKATATND-AISQQKHRRP----- 60

Query: 61  FASANIQDDSHRRCSVAVDVGHNFSAKYVPFGSGYDSSEWYSLDEIVYRSRSGGLLDVQH 120
            A  NI++++ R CS      HNFSA+YVPF +G +S EWYSLDEIVYRSRSGGLLDVQH
Sbjct: 61  -ADENIREEARRHCS-----SHNFSARYVPFNAGPNSDEWYSLDEIVYRSRSGGLLDVQH 120

Query: 121 NMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLFW 180
           +M+ALKKFDG YWR+LFDSRVGKTTWPYGSGVWSKKEWVLPEIDS DIVSAFEGNSNLFW
Sbjct: 121 DMDALKKFDGQYWRSLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFW 180

Query: 181 AERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVFGVGCASTGDT 240
           AERFGKQFLGM DLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKM++PV GVGCASTGDT
Sbjct: 181 AERFGKQFLGMTDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCASTGDT 240

Query: 241 SAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAEL 300
           SAALSAYCA+AGIPSIVFLPA+KIS+AQLVQPIANGAFVLS+DTDFDGCM+LIREVTAEL
Sbjct: 241 SAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMQLIREVTAEL 300

Query: 301 PIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELGL 360
           PIYLANSLNSLRLEGQKTAAIEILQQFDWEVP+WVI+PGGNLGNIYAFYKGFQMCKELGL
Sbjct: 301 PIYLANSLNSLRLEGQKTAAIEILQQFDWEVPEWVIVPGGNLGNIYAFYKGFQMCKELGL 360

Query: 361 VDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALKNS 420
           VD+IPRLVCAQAANANPLYL+YKSGWK+F P+KA TTFASAIQIGDPVSIDRAVFAL+  
Sbjct: 361 VDRIPRLVCAQAANANPLYLHYKSGWKDFKPVKANTTFASAIQIGDPVSIDRAVFALQQC 420

Query: 421 GGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIGASDRTVVVSTAHG 480
            GIVEEATEEELMDA AQADSTGMFICPHTGVALTAL KLRN GVI  +DRTVVVSTAHG
Sbjct: 421 NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNSGVIAPTDRTVVVSTAHG 480

Query: 481 LKFTQSKIDYHSRAIPGMECRYANPPVEVKAEVGAVMDVLKEHLLKKTPK 529
           LKFTQSKIDYHS+ I  MECR+ANPPVEVKA+ G+VMDVLK +LL +  K
Sbjct: 481 LKFTQSKIDYHSKEIKDMECRFANPPVEVKADFGSVMDVLKSYLLSQNSK 518

BLAST of Bhi06G001397 vs. ExPASy Swiss-Prot
Match: Q9S7B5 (Threonine synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS1 PE=1 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 1.1e-234
Identity = 415/530 (78.30%), Postives = 457/530 (86.23%), Query Frame = 0

Query: 1   MASSLFSFNTSFST---KPDSL-FHRSARRNSSSPFTVVCSSATSDSAASPVLHSSPPLG 60
           MASS   FN S S+   K D +  HRS       P  + C++  ++  A       PP  
Sbjct: 1   MASSCL-FNASVSSLNPKQDPIRRHRSTSLLRHRPVVISCTADGNNIKAPIETAVKPP-- 60

Query: 61  HQFASANIQDDSHRRCSVAVDVGHNFSAKYVPFGSGYDSSEWYSLDEIVYRSRSGGLLDV 120
           H+    NI+D++ R  S AV   + FSAKYVPF +   S+E YSLDEIVYRSRSGGLLDV
Sbjct: 61  HR-TEDNIRDEARRNRSNAV---NPFSAKYVPFNAAPGSTESYSLDEIVYRSRSGGLLDV 120

Query: 121 QHNMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNL 180
           +H+MEALK+FDGAYWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEID  DIVSAFEGNSNL
Sbjct: 121 EHDMEALKRFDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNL 180

Query: 181 FWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVFGVGCASTG 240
           FWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKM RPV GVGCASTG
Sbjct: 181 FWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCASTG 240

Query: 241 DTSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTA 300
           DTSAALSAYCA+AGIPSIVFLPA+KIS+AQLVQPIANGAFVLS+DTDFDGCMKLIRE+TA
Sbjct: 241 DTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREITA 300

Query: 301 ELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKEL 360
           ELPIYLANSLNSLRLEGQKTAAIEILQQFDW+VPDWVI+PGGNLGNIYAFYKGF+MC+EL
Sbjct: 301 ELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQEL 360

Query: 361 GLVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALK 420
           GLVD+IPR+VCAQAANANPLYL+YKSGWK+F P+ A+TTFASAIQIGDPVSIDRAV+ALK
Sbjct: 361 GLVDRIPRMVCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYALK 420

Query: 421 NSGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIGASDRTVVVSTA 480
              GIVEEATEEELMDA AQADSTGMFICPHTGVALTAL KLRN+GVI  +DRTVVVSTA
Sbjct: 421 KCNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVSTA 480

Query: 481 HGLKFTQSKIDYHSRAIPGMECRYANPPVEVKAEVGAVMDVLKEHLLKKT 527
           HGLKFTQSKIDYHS AIP M CR++NPPV+VKA+ GAVMDVLK +L   T
Sbjct: 481 HGLKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYLGSNT 523

BLAST of Bhi06G001397 vs. ExPASy Swiss-Prot
Match: Q9SSP5 (Threonine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS2 PE=1 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 1.9e-228
Identity = 401/501 (80.04%), Postives = 440/501 (87.82%), Query Frame = 0

Query: 23  SARRNSSSPFTVVCSSATSDSAASPVLHSSPPLGHQFASANIQDDSHRRCSVAVDVGHNF 82
           S + +S++ FTV C+SA   S A P    +P    +    NI+D++ RR         N 
Sbjct: 20  SLKPHSAASFTVRCTSA---SPAVP--PQTPQKPRRSPDENIRDEARRRPHQL----QNL 79

Query: 83  SAKYVPFGSGYDSSEWYSLDEIVYRSRSGGLLDVQHNMEALKKFDGAYWRNLFDSRVGKT 142
           SA+YVPF +   S+E YSLDEIVYRS+SG LLDVQH+  ALK++DG +WRNLFDSRVGKT
Sbjct: 80  SARYVPFNAPPSSTESYSLDEIVYRSQSGALLDVQHDFAALKRYDGEFWRNLFDSRVGKT 139

Query: 143 TWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHT 202
            WPYGSGVWSKKEWVLPEID  DIVSAFEGNSNLFWAERFGKQ+L MNDLWVKHCGISHT
Sbjct: 140 NWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFWAERFGKQYLQMNDLWVKHCGISHT 199

Query: 203 GSFKDLGMTVLVSQVNRLRKMNRPVFGVGCASTGDTSAALSAYCAAAGIPSIVFLPADKI 262
           GSFKDLGM+VLVSQVNRLRKMN+PV GVGCASTGDTSAALSAYCA+AGIPSIVFLPADKI
Sbjct: 200 GSFKDLGMSVLVSQVNRLRKMNKPVIGVGCASTGDTSAALSAYCASAGIPSIVFLPADKI 259

Query: 263 SIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEIL 322
           S+AQLVQPIANGAFVLS+DTDFDGCM LIREVTAELPIYLANSLNSLRLEGQKTAAIEIL
Sbjct: 260 SMAQLVQPIANGAFVLSIDTDFDGCMHLIREVTAELPIYLANSLNSLRLEGQKTAAIEIL 319

Query: 323 QQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELGLVDKIPRLVCAQAANANPLYLYYKS 382
           QQF+W+VPDWVI+PGGNLGNIYAFYKGF MCKELGLVD+IPRLVCAQAANANPLYL+YKS
Sbjct: 320 QQFNWQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDRIPRLVCAQAANANPLYLHYKS 379

Query: 383 GWKE-FSPLKATTTFASAIQIGDPVSIDRAVFALKNSGGIVEEATEEELMDATAQADSTG 442
           G+KE F+PLKA TTFASAIQIGDPVSIDRAV+ALK S GIVEEATEEELMDATA ADSTG
Sbjct: 380 GFKEDFNPLKANTTFASAIQIGDPVSIDRAVYALKKSNGIVEEATEEELMDATALADSTG 439

Query: 443 MFICPHTGVALTALIKLRNRGVIGASDRTVVVSTAHGLKFTQSKIDYHSRAIPGMECRYA 502
           MFICPHTGVALTAL+KLR  GVIGA+DRTVVVSTAHGLKFTQSKIDYHS+ I  M CR A
Sbjct: 440 MFICPHTGVALTALMKLRKSGVIGANDRTVVVSTAHGLKFTQSKIDYHSKNIKEMACRLA 499

Query: 503 NPPVEVKAEVGAVMDVLKEHL 523
           NPPV+VKA+ G+VMDVLKE+L
Sbjct: 500 NPPVKVKAKFGSVMDVLKEYL 511

BLAST of Bhi06G001397 vs. ExPASy Swiss-Prot
Match: Q58860 (Threonine synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=thrC PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 5.1e-72
Identity = 158/423 (37.35%), Postives = 245/423 (57.92%), Query Frame = 0

Query: 99  YSLDEIVYRSRSGGLLDVQHNMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVL 158
           Y +DEI+Y    GGLL++ ++ E +K         + + ++ K       GVW   E+ L
Sbjct: 12  YDVDEIIYTCECGGLLEIIYDYEEIKD-------KVSEEKLRKRE----IGVWRYLEY-L 71

Query: 159 PEIDSSDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVN 218
           P  D S IVS  EG + L+      K+ LG+ +L+VK+ G + TGSFKD GMTV V++ N
Sbjct: 72  PVKDESKIVSLCEGGTPLYRCNNLEKE-LGIKELYVKNEGANPTGSFKDRGMTVGVTRAN 131

Query: 219 RLRKMNRPVFGVGCASTGDTSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVL 278
            L      V  VGCASTG+TSA+L+AY A +G   IV LP  K+++ +L Q +  GA V+
Sbjct: 132 EL-----GVEVVGCASTGNTSASLAAYSARSGKKCIVLLPEGKVALGKLAQAMFYGAKVI 191

Query: 279 SMDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGG 338
            +  +FD  + +++++  E  IYL NS+N  RLEGQKT A EI  Q +W+VPD VI+P G
Sbjct: 192 QVKGNFDDALDMVKQLAKEKLIYLLNSINPFRLEGQKTIAFEICDQLNWQVPDRVIVPVG 251

Query: 339 NLGNIYAFYKGFQMCKELGLVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFAS 398
           N GNI A +KGF+  +  G++D++P++   QA  A P+   ++   K+  P K   T A+
Sbjct: 252 NAGNISAIWKGFKEFEITGIIDELPKMTGIQADGAKPIVEAFRKRAKDIIPYKNPETIAT 311

Query: 399 AIQIGDPVSIDRAVFALKNSGGIVEEATEEELMDA-TAQADSTGMFICPHTGVALTALIK 458
           AI+IG+PV+  +A+ A+ +SGG  E  T+EE+++A    A   G+F+ P +  ++  L K
Sbjct: 312 AIRIGNPVNAPKALDAIYSSGGYAEAVTDEEIVEAQKLLARKEGIFVEPASASSIAGLKK 371

Query: 459 LRNRGVIGASDRTVVVSTAHGLKFTQSKIDYHSRAIPGMECRYANPPVEVKAEVGAVMDV 518
           L   G+I   +R V ++T HGLK             P    R +  P++++ ++  +  +
Sbjct: 372 LLEEGIIDRDERIVCITTGHGLK------------DPDAAIRASEEPIKIECDMNVLKRI 404

Query: 519 LKE 521
           LKE
Sbjct: 432 LKE 404

BLAST of Bhi06G001397 vs. ExPASy Swiss-Prot
Match: Q8TQD4 (Threonine synthase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=thrC PE=1 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.4e-66
Identity = 157/391 (40.15%), Postives = 232/391 (59.34%), Query Frame = 0

Query: 99  YSLDEIVYR-SRSGGLLDVQHNMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWV 158
           YS DE++Y  S+  GLLDV ++  ++K           D    KT  P    VW K   +
Sbjct: 13  YSRDEVIYTCSKCDGLLDVIYDYSSIK----------IDMEKLKTECP---SVW-KYAKL 72

Query: 159 LPEIDSSDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQV 218
           LP     + V+  EG + L+  +R  ++ +G+  L+VKH G++ TGSFKD GMTV V++ 
Sbjct: 73  LPV--EREPVTIQEGGTPLYKCDRLAEK-IGIKKLYVKHEGMNPTGSFKDRGMTVGVTKA 132

Query: 219 NRLRKMNRPVFGVGCASTGDTSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFV 278
             L  MN     V CASTG+TSAAL+ Y A AGIP +V LPA K+++ ++ Q + +GA V
Sbjct: 133 LEL-GMNT----VACASTGNTSAALAIYGAKAGIPVVVLLPAGKVALGKVAQALMHGAKV 192

Query: 279 LSMDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPG 338
           LS+  +FD  + L+R + ++  IYL NS+N  RLEGQKT   EI  Q D++VPD +++P 
Sbjct: 193 LSIRGNFDDALALVRTLCSQEKIYLLNSINPYRLEGQKTIGFEIADQLDFKVPDRIVLPV 252

Query: 339 GNLGNIYAFYKGFQMCKELGLVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFA 398
           GN GNI A YKGF+  K LG+ D +P++   QA  + P+    KSG    +P +   T A
Sbjct: 253 GNAGNITAIYKGFREFKILGITDSLPKMTGIQAEGSCPIVKAIKSGAPAITPEENPETVA 312

Query: 399 SAIQIGDPVSIDRAVFALKNSGGIVEEATEEELMDATAQ-ADSTGMFICPHTGVALTALI 458
           +AI+IG+PV+  +A+ A++ SGG  E  T+EE++ A    A   G+ + P +  ++  L 
Sbjct: 313 TAIRIGNPVNATKALSAIRESGGTAESVTDEEILAAQKDLARLEGIGVEPASAASVAGLR 372

Query: 459 KLRNRGVIGASDRTVVVSTAHGLKFTQSKID 488
           KL + GVIG  +  V ++T H LK  Q+ ID
Sbjct: 373 KLVDMGVIGRDETVVCITTGHLLKDPQTVID 381

BLAST of Bhi06G001397 vs. ExPASy TrEMBL
Match: A0A5A7V3M7 (Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G00370 PE=3 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 3.0e-285
Identity = 494/532 (92.86%), Postives = 506/532 (95.11%), Query Frame = 0

Query: 1   MASSLFSFNTSFSTKPDSLFHRSARRNSSSPFTVVCSSATSDSAASPVLHSSPPLG---H 60
           MASSLF FNTS S KPDSL  R  RRNSSSPFT+VCSSATSDSA SPV+ SS PLG   H
Sbjct: 1   MASSLFPFNTSLSPKPDSLLRRPTRRNSSSPFTLVCSSATSDSAPSPVIDSSLPLGVHTH 60

Query: 61  QFASANIQDDSHRRCSVAVDVGHNFSAKYVPFGSGYDSSEWYSLDEIVYRSRSGGLLDVQ 120
           QF     QDDSHR  SVAVDVGHNFSAKYVPFGSGYDSSEWYSLD+IVYRSRSGGLLDVQ
Sbjct: 61  QF-----QDDSHRHRSVAVDVGHNFSAKYVPFGSGYDSSEWYSLDDIVYRSRSGGLLDVQ 120

Query: 121 HNMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180
           HNM+ALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGN+NLF
Sbjct: 121 HNMDALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNTNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVFGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPV GVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLPADKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGF+MCKELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFEMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFAL+N
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALQN 420

Query: 421 SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIGASDRTVVVSTAH 480
           S GIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRG+IG SDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGIIGGSDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPGMECRYANPPVEVKAEVGAVMDVLKEHLLKKTPKF 530
           GLKFTQSKIDYHSRAI GMECR+ANPPVEVKAE GAVMDVLKEHL +K+PKF
Sbjct: 481 GLKFTQSKIDYHSRAISGMECRFANPPVEVKAEFGAVMDVLKEHLSRKSPKF 527

BLAST of Bhi06G001397 vs. ExPASy TrEMBL
Match: A0A1S3CB26 (Threonine synthase OS=Cucumis melo OX=3656 GN=LOC103498677 PE=3 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 3.0e-285
Identity = 494/532 (92.86%), Postives = 506/532 (95.11%), Query Frame = 0

Query: 1   MASSLFSFNTSFSTKPDSLFHRSARRNSSSPFTVVCSSATSDSAASPVLHSSPPLG---H 60
           MASSLF FNTS S KPDSL  R  RRNSSSPFT+VCSSATSDSA SPV+ SS PLG   H
Sbjct: 1   MASSLFPFNTSLSPKPDSLLRRPTRRNSSSPFTLVCSSATSDSAPSPVIDSSLPLGVHTH 60

Query: 61  QFASANIQDDSHRRCSVAVDVGHNFSAKYVPFGSGYDSSEWYSLDEIVYRSRSGGLLDVQ 120
           QF     QDDSHR  SVAVDVGHNFSAKYVPFGSGYDSSEWYSLD+IVYRSRSGGLLDVQ
Sbjct: 61  QF-----QDDSHRHRSVAVDVGHNFSAKYVPFGSGYDSSEWYSLDDIVYRSRSGGLLDVQ 120

Query: 121 HNMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180
           HNM+ALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGN+NLF
Sbjct: 121 HNMDALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNTNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVFGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPV GVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLPADKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGF+MCKELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFEMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFAL+N
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALQN 420

Query: 421 SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIGASDRTVVVSTAH 480
           S GIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRG+IG SDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGIIGGSDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPGMECRYANPPVEVKAEVGAVMDVLKEHLLKKTPKF 530
           GLKFTQSKIDYHSRAI GMECR+ANPPVEVKAE GAVMDVLKEHL +K+PKF
Sbjct: 481 GLKFTQSKIDYHSRAISGMECRFANPPVEVKAEFGAVMDVLKEHLSRKSPKF 527

BLAST of Bhi06G001397 vs. ExPASy TrEMBL
Match: A0A5D3CFM9 (Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001120 PE=3 SV=1)

HSP 1 Score: 988.8 bits (2555), Expect = 8.8e-285
Identity = 493/532 (92.67%), Postives = 506/532 (95.11%), Query Frame = 0

Query: 1   MASSLFSFNTSFSTKPDSLFHRSARRNSSSPFTVVCSSATSDSAASPVLHSSPPLG---H 60
           MASSLF FNTS S KPDSL  R  RRNSSSPFT+VCSSATSDSA SPV+ SS PLG   H
Sbjct: 1   MASSLFPFNTSLSPKPDSLLRRPTRRNSSSPFTLVCSSATSDSAPSPVIDSSLPLGVHTH 60

Query: 61  QFASANIQDDSHRRCSVAVDVGHNFSAKYVPFGSGYDSSEWYSLDEIVYRSRSGGLLDVQ 120
           QF     QDDSHR  SVAVDVGHNFSAKYVPFGSGYDSSEWYSLD+IVYRSRSGGLLDVQ
Sbjct: 61  QF-----QDDSHRHRSVAVDVGHNFSAKYVPFGSGYDSSEWYSLDDIVYRSRSGGLLDVQ 120

Query: 121 HNMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180
           HNM+ALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGN+NLF
Sbjct: 121 HNMDALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNTNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVFGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPV GVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLPADKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGF+MCKELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFEMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGW+EFSPLKATTTFASAIQIGDPVSIDRAVFAL+N
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWEEFSPLKATTTFASAIQIGDPVSIDRAVFALQN 420

Query: 421 SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIGASDRTVVVSTAH 480
           S GIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRG+IG SDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGIIGGSDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPGMECRYANPPVEVKAEVGAVMDVLKEHLLKKTPKF 530
           GLKFTQSKIDYHSRAI GMECR+ANPPVEVKAE GAVMDVLKEHL +K+PKF
Sbjct: 481 GLKFTQSKIDYHSRAISGMECRFANPPVEVKAEFGAVMDVLKEHLSRKSPKF 527

BLAST of Bhi06G001397 vs. ExPASy TrEMBL
Match: A0A6J1G4S3 (Threonine synthase OS=Cucurbita moschata OX=3662 GN=LOC111450711 PE=3 SV=1)

HSP 1 Score: 985.3 bits (2546), Expect = 9.7e-284
Identity = 492/533 (92.31%), Postives = 504/533 (94.56%), Query Frame = 0

Query: 1   MASSLFSFNTSFSTKPDSLFHRSARRNSSSPFTVVCSSATSDSAASPVLHSSPPLG---H 60
           MASSLFSFNTS S KP SLF R  RRNSS PFT+ C SATSDSAASPVL SS PLG    
Sbjct: 1   MASSLFSFNTSLSPKPSSLFRRPLRRNSSPPFTIACPSATSDSAASPVLDSSLPLGLQNP 60

Query: 61  QFASANIQDDSHRRCSVAVDVGHNFSAKYVPFGSGYDSSEWYSLDEIVYRSRSGGLLDVQ 120
           QFAS +IQDDSHR  SVA D G NFSAKYVPFGSG+DSSEWYSLDEIVYRS+SGGLLDVQ
Sbjct: 61  QFASVDIQDDSHRSRSVAADGGRNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120

Query: 121 HNMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180
           HNMEALK+FDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF
Sbjct: 121 HNMEALKEFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVFGVGCASTGD 240
           WAERFGK+FLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPV GVGCASTGD
Sbjct: 181 WAERFGKEFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLPADKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIRE+TAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKA+TTFASAIQIGDPVSIDRAVFALKN
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420

Query: 421 SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIGASDRTVVVSTAH 480
           SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVI  SDRTVVVSTAH
Sbjct: 421 SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPGMECRYANPPVEVKAEVGAVMDVLKEHLLKKTPKFE 531
           GLKFTQSKIDYHSRAI GMECR+ANPPVEVKA+ GAVMDVLKEHL +K PK E
Sbjct: 481 GLKFTQSKIDYHSRAIQGMECRFANPPVEVKADFGAVMDVLKEHLSRKNPKME 533

BLAST of Bhi06G001397 vs. ExPASy TrEMBL
Match: A0A0A0LHR0 (Threonine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G000280 PE=3 SV=1)

HSP 1 Score: 983.4 bits (2541), Expect = 3.7e-283
Identity = 493/533 (92.50%), Postives = 503/533 (94.37%), Query Frame = 0

Query: 1   MASSLFSFNTSFSTKPDSLFHRSARRNSSSPFTVVCSSATSDSAASPVLHSSPPLG---H 60
           MASSLFSFNTS S KPDSLF R  RRNSSSPFT+VCSSATSDSA S V+ SS PLG   H
Sbjct: 1   MASSLFSFNTSLSPKPDSLFRRPTRRNSSSPFTLVCSSATSDSAPSHVIDSSLPLGVHTH 60

Query: 61  QFASANIQDDSHRRCSVAVDVGHNFSAKYVPFGSGYDSSEWYSLDEIVYRSRSGGLLDVQ 120
           QF     QDDSHR  SVAVD   NFSAKYVPFGSGYDSSEWYSLD+IVYRSRSGGLLDVQ
Sbjct: 61  QF-----QDDSHRHRSVAVDADRNFSAKYVPFGSGYDSSEWYSLDDIVYRSRSGGLLDVQ 120

Query: 121 HNMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180
           HNM+ALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF
Sbjct: 121 HNMDALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVFGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPV GVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLPADKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGF+MCKELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFEMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFAL+N
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALQN 420

Query: 421 SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIGASDRTVVVSTAH 480
           S GIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRG+IG +DRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGIIGGNDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPGMECRYANPPVEVKAEVGAVMDVLKEHLLKKTPKFE 531
           GLKFTQSKIDYHSRAI GMECR+ANPPVEVKAE GAVMDVLKEHL  K PKFE
Sbjct: 481 GLKFTQSKIDYHSRAISGMECRFANPPVEVKAEFGAVMDVLKEHLSGKNPKFE 528

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G29840.17.5e-23678.30Pyridoxal-5'-phosphate-dependent enzyme family protein [more]
AT1G72810.11.4e-22980.04Pyridoxal-5'-phosphate-dependent enzyme family protein [more]
Match NameE-valueIdentityDescription
Q9MT289.8e-24179.25Threonine synthase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
Q9S7B51.1e-23478.30Threonine synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS1 PE=1 ... [more]
Q9SSP51.9e-22880.04Threonine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS2 PE=1 ... [more]
Q588605.1e-7237.35Threonine synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 266... [more]
Q8TQD41.4e-6640.15Threonine synthase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 /... [more]
Match NameE-valueIdentityDescription
A0A5A7V3M73.0e-28592.86Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G00... [more]
A0A1S3CB263.0e-28592.86Threonine synthase OS=Cucumis melo OX=3656 GN=LOC103498677 PE=3 SV=1[more]
A0A5D3CFM98.8e-28592.67Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G0... [more]
A0A6J1G4S39.7e-28492.31Threonine synthase OS=Cucurbita moschata OX=3662 GN=LOC111450711 PE=3 SV=1[more]
A0A0A0LHR03.7e-28392.50Threonine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G000280 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 205..306
e-value: 1.7E-106
score: 358.0
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 157..483
e-value: 1.7E-106
score: 358.0
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeSUPERFAMILY53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 98..521
IPR004450Threonine synthase-likeTIGRFAMTIGR00260TIGR00260coord: 156..482
e-value: 5.7E-61
score: 204.5
IPR001926Pyridoxal-phosphate dependent enzymePFAMPF00291PALPcoord: 169..475
e-value: 9.4E-48
score: 163.1
NoneNo IPR availablePIRSRPIRSR038945-1PIRSR038945-1coord: 149..463
e-value: 6.9E-70
score: 233.6
NoneNo IPR availablePIRSRPIRSR038945-2PIRSR038945-2coord: 149..463
e-value: 6.9E-70
score: 233.6
NoneNo IPR availablePANTHERPTHR10314CYSTATHIONINE BETA-SYNTHASEcoord: 32..525
NoneNo IPR availablePANTHERPTHR10314:SF176THREONINE SYNTHASE, CHLOROPLASTIC-LIKE ISOFORM X1coord: 32..525
NoneNo IPR availableCDDcd01563Thr-synth_1coord: 151..481
e-value: 9.01289E-147
score: 422.387
IPR000634Serine/threonine dehydratase, pyridoxal-phosphate-binding sitePROSITEPS00165DEHYDRATASE_SER_THRcoord: 196..210

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi06M001397Bhi06M001397mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019344 cysteine biosynthetic process
biological_process GO:0009088 threonine biosynthetic process
biological_process GO:0006520 cellular amino acid metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0004795 threonine synthase activity