Bhi06G001351 (gene) Wax gourd (B227) v1

Overview
NameBhi06G001351
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionUPF0481 protein At3g47200-like
Locationchr6: 45394949 .. 45397323 (+)
RNA-Seq ExpressionBhi06G001351
SyntenyBhi06G001351
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTATTTTTTATTTTTCTTTCAGTTCATATACTCTCCCCATTTTTCTTTCCTTAACTGTCTCTTCATTTCATCCCTCATTTTCATTTACTACCAACATCTTCTTCCATCAATTTAGACACAAGGTACATCTTTATTTCTTCCTTCTCTTTTTCTTAGATGATTTCTTTTTAAGGGACCATGTTGAATTCTTAGCTAATTTATTGTTAATTTGATCAATCATGTATCTCTCGAAAACTAAAAACTATGTGATTTGTAATAATTACATCTTTAAATTTCTGATTCGATCCATTAGCTAGCTTTTGTAGTTTGTTATTGGTTACAATCAACCCCTGTGTTAGTATTGATTATATTCGATATATTTGCAAAATTAAAATTTTGATATCAACATTATTTGACATCAATTCATTTTACCCGTTTTCGGTTTAAATTTAGCTATACAATCAACCCATCTAATGAAAACACTACTAACTCAAAACAAAAATAAACTTTTTAAAAATTACTTTCTTTTAGTTTTCAAATTTTGACTTGGTTTTTGAAAACATGGGTAAAAAGTTAGATATCAAAGCAAGAGATTTAGAGGTGAAAAAGGTGTTCTTAAACTTAATTTTCAAAAAAACAAAAACAAAAAATGAAGTGATTACTGAATGGTGTCTAAAAAGCAACGAGACCCTAATTTACTAGCAAGTTGTGTTTGAAAGTTGAATCATTCCTCTGTTTATTTATTATTTAGTTGGTTTTTAAAATATATATGCATGGCTCTTGGTTGAAGGAAACTTGAAAACGTGGGTGGGTTTGAATATGGAAATGAGTGAGATTGAAAAGCAAGAAGCAAACGACATAACTCACAGTAGAGTGGGAGAAATTAGTGAGGCTGATCAAAGGCTTTGCGGTAATGTTGTGATATGTATTGAAAAAATGTTGGAACAACTACCTCAAGTTAATGCAAAATGCAGCATCTATCGAATTTCCAAAGAGCTATGCGAGGTTAATCGTAAAGCCTATACCCCTCAACTCATTTCTATCGGCCCTATTCACTGTGGTACTCATAAAGATTTGATCGCCAATGAACCATATAAGCTTCAAGGTTTTATTAACTTCCTACGTCGTATCAACAACAACAAGATTGAATCAATGGAGGACCTCTTAAAAACTGGAGCAGTGAATATCCTTGTGGAAAAAGCTCATCATTGGGTGAAAAAAGCTCGCAATTGCTATGCAACTCCCACAAACATGATGGATATTGATGAGTTTGTTATGATGATGCTTGTGGATGCTTGTTTCGTAGTGGAGTTTCTGATACTAAAATTTGACTGCGACCATCCAGATTGTAAGTTCTTTCAAATTCAAGACAACGTAGATATTTCATTCTACCAAGGAATAGACTTGCATATACTTTATGATTTGATAAAGCTGGAAAATCAAGTTCCTTTCTTTCTTCTTCGGAATTTGTTTAACCTGATACCAAAACATGATGTCCCCATGATAATCTTCTCCTTTGGAGATCTTACACACAAGGCTCTTAAGTTTCGGTTGGTGCGTAAATATGAGATTGATTTGTATAAAGAACCAAAGCATTTGGTTGATCTCTTAAGTTTCTATTTTGTCCCTTTGCCCCTTGATGATATGCAAAACAAGCATCATGGATTCAAAAATGGCGAGGAACATAGATGGATTCCTCCATCGATAACTGAGCTTTGCGAGGCTGGTGTCACCATCAAGAAGGCCGAAAACGCCAAATATGTAACCAACATAACCTTCAAAAATGGGGTTTTGGAAATCCCACCTTTACATATTTACGATGAATTTGAACTTATGTTACGAAACATGGTAGCATTTGAGCAAATCTCCGCAGGAAAGAAGAACAAGTATGTAATCCAATATGTGTTATTTATGGATGATTTGATAAGTACAGAGAAAGATGTGCGTTTACTTGTGGAGGCCGGAGTCATAATCAACCAGATTGGCGGTAGTGATAAAGAAGTTTCAGATCTATTTAACAATCTCTGTAAATTTATCACAGCCCCGAGATCTTCTCACTTCGACCCTATCATCAAAGATTTATGTGATCATTGCAATGGAAGGTGGAACAAGGCGAAAGCTTCACTAAAACATAACTATTTCAATACGCCATGGGCCTTTATCTCCCTTTTTGCTGCGTCCTTACTCATTCTTCTCACCATTCTTCAAACCATTTTCTCTGCTATCTCAGCATTTCCTAGCGCCTAATCAGACCTGTCCCTACATTTATGTTGTATTCTATGTTTCTTCCCTTTTTAATTACTTGCTTCTTTCACCTTCTTCAAGCTTAGCCTTGCATTTAGATAAGCATGATATTCTATATTAAAACCCGGTTGACATTGAATGAAAAGTAG

mRNA sequence

TTTTTATTTTTTATTTTTCTTTCAGTTCATATACTCTCCCCATTTTTCTTTCCTTAACTGTCTCTTCATTTCATCCCTCATTTTCATTTACTACCAACATCTTCTTCCATCAATTTAGACACAAGGAAACTTGAAAACGTGGGTGGGTTTGAATATGGAAATGAGTGAGATTGAAAAGCAAGAAGCAAACGACATAACTCACAGTAGAGTGGGAGAAATTAGTGAGGCTGATCAAAGGCTTTGCGGTAATGTTGTGATATGTATTGAAAAAATGTTGGAACAACTACCTCAAGTTAATGCAAAATGCAGCATCTATCGAATTTCCAAAGAGCTATGCGAGGTTAATCGTAAAGCCTATACCCCTCAACTCATTTCTATCGGCCCTATTCACTGTGGTACTCATAAAGATTTGATCGCCAATGAACCATATAAGCTTCAAGGTTTTATTAACTTCCTACGTCGTATCAACAACAACAAGATTGAATCAATGGAGGACCTCTTAAAAACTGGAGCAGTGAATATCCTTGTGGAAAAAGCTCATCATTGGGTGAAAAAAGCTCGCAATTGCTATGCAACTCCCACAAACATGATGGATATTGATGAGTTTGTTATGATGATGCTTGTGGATGCTTGTTTCGTAGTGGAGTTTCTGATACTAAAATTTGACTGCGACCATCCAGATTGTAAGTTCTTTCAAATTCAAGACAACGTAGATATTTCATTCTACCAAGGAATAGACTTGCATATACTTTATGATTTGATAAAGCTGGAAAATCAAGTTCCTTTCTTTCTTCTTCGGAATTTGTTTAACCTGATACCAAAACATGATGTCCCCATGATAATCTTCTCCTTTGGAGATCTTACACACAAGGCTCTTAAGTTTCGGTTGGTGCGTAAATATGAGATTGATTTGTATAAAGAACCAAAGCATTTGGTTGATCTCTTAAGTTTCTATTTTGTCCCTTTGCCCCTTGATGATATGCAAAACAAGCATCATGGATTCAAAAATGGCGAGGAACATAGATGGATTCCTCCATCGATAACTGAGCTTTGCGAGGCTGGTGTCACCATCAAGAAGGCCGAAAACGCCAAATATGTAACCAACATAACCTTCAAAAATGGGGTTTTGGAAATCCCACCTTTACATATTTACGATGAATTTGAACTTATGTTACGAAACATGGTAGCATTTGAGCAAATCTCCGCAGGAAAGAAGAACAAGTATGTAATCCAATATGTGTTATTTATGGATGATTTGATAAGTACAGAGAAAGATGTGCGTTTACTTGTGGAGGCCGGAGTCATAATCAACCAGATTGGCGGTAGTGATAAAGAAGTTTCAGATCTATTTAACAATCTCTGTAAATTTATCACAGCCCCGAGATCTTCTCACTTCGACCCTATCATCAAAGATTTATGTGATCATTGCAATGGAAGGTGGAACAAGGCGAAAGCTTCACTAAAACATAACTATTTCAATACGCCATGGGCCTTTATCTCCCTTTTTGCTGCGTCCTTACTCATTCTTCTCACCATTCTTCAAACCATTTTCTCTGCTATCTCAGCATTTCCTAGCGCCTAATCAGACCTGTCCCTACATTTATGTTGTATTCTATGTTTCTTCCCTTTTTAATTACTTGCTTCTTTCACCTTCTTCAAGCTTAGCCTTGCATTTAGATAAGCATGATATTCTATATTAAAACCCGGTTGACATTGAATGAAAAGTAG

Coding sequence (CDS)

ATGGAAATGAGTGAGATTGAAAAGCAAGAAGCAAACGACATAACTCACAGTAGAGTGGGAGAAATTAGTGAGGCTGATCAAAGGCTTTGCGGTAATGTTGTGATATGTATTGAAAAAATGTTGGAACAACTACCTCAAGTTAATGCAAAATGCAGCATCTATCGAATTTCCAAAGAGCTATGCGAGGTTAATCGTAAAGCCTATACCCCTCAACTCATTTCTATCGGCCCTATTCACTGTGGTACTCATAAAGATTTGATCGCCAATGAACCATATAAGCTTCAAGGTTTTATTAACTTCCTACGTCGTATCAACAACAACAAGATTGAATCAATGGAGGACCTCTTAAAAACTGGAGCAGTGAATATCCTTGTGGAAAAAGCTCATCATTGGGTGAAAAAAGCTCGCAATTGCTATGCAACTCCCACAAACATGATGGATATTGATGAGTTTGTTATGATGATGCTTGTGGATGCTTGTTTCGTAGTGGAGTTTCTGATACTAAAATTTGACTGCGACCATCCAGATTGTAAGTTCTTTCAAATTCAAGACAACGTAGATATTTCATTCTACCAAGGAATAGACTTGCATATACTTTATGATTTGATAAAGCTGGAAAATCAAGTTCCTTTCTTTCTTCTTCGGAATTTGTTTAACCTGATACCAAAACATGATGTCCCCATGATAATCTTCTCCTTTGGAGATCTTACACACAAGGCTCTTAAGTTTCGGTTGGTGCGTAAATATGAGATTGATTTGTATAAAGAACCAAAGCATTTGGTTGATCTCTTAAGTTTCTATTTTGTCCCTTTGCCCCTTGATGATATGCAAAACAAGCATCATGGATTCAAAAATGGCGAGGAACATAGATGGATTCCTCCATCGATAACTGAGCTTTGCGAGGCTGGTGTCACCATCAAGAAGGCCGAAAACGCCAAATATGTAACCAACATAACCTTCAAAAATGGGGTTTTGGAAATCCCACCTTTACATATTTACGATGAATTTGAACTTATGTTACGAAACATGGTAGCATTTGAGCAAATCTCCGCAGGAAAGAAGAACAAGTATGTAATCCAATATGTGTTATTTATGGATGATTTGATAAGTACAGAGAAAGATGTGCGTTTACTTGTGGAGGCCGGAGTCATAATCAACCAGATTGGCGGTAGTGATAAAGAAGTTTCAGATCTATTTAACAATCTCTGTAAATTTATCACAGCCCCGAGATCTTCTCACTTCGACCCTATCATCAAAGATTTATGTGATCATTGCAATGGAAGGTGGAACAAGGCGAAAGCTTCACTAAAACATAACTATTTCAATACGCCATGGGCCTTTATCTCCCTTTTTGCTGCGTCCTTACTCATTCTTCTCACCATTCTTCAAACCATTTTCTCTGCTATCTCAGCATTTCCTAGCGCCTAA

Protein sequence

MEMSEIEKQEANDITHSRVGEISEADQRLCGNVVICIEKMLEQLPQVNAKCSIYRISKELCEVNRKAYTPQLISIGPIHCGTHKDLIANEPYKLQGFINFLRRINNNKIESMEDLLKTGAVNILVEKAHHWVKKARNCYATPTNMMDIDEFVMMMLVDACFVVEFLILKFDCDHPDCKFFQIQDNVDISFYQGIDLHILYDLIKLENQVPFFLLRNLFNLIPKHDVPMIIFSFGDLTHKALKFRLVRKYEIDLYKEPKHLVDLLSFYFVPLPLDDMQNKHHGFKNGEEHRWIPPSITELCEAGVTIKKAENAKYVTNITFKNGVLEIPPLHIYDEFELMLRNMVAFEQISAGKKNKYVIQYVLFMDDLISTEKDVRLLVEAGVIINQIGGSDKEVSDLFNNLCKFITAPRSSHFDPIIKDLCDHCNGRWNKAKASLKHNYFNTPWAFISLFAASLLILLTILQTIFSAISAFPSA
Homology
BLAST of Bhi06G001351 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 214.9 bits (546), Expect = 1.4e-55
Identity = 144/442 (32.58%), Postives = 237/442 (53.62%), Query Frame = 0

Query: 33  VVICIEKMLEQLPQVNAKCSIYRISKELCEVNRKAYTPQLISIGPIHCGTHKDLIANEPY 92
           +V  I+  L  L  ++ KC IY++  +L  +N  AYTP+L+S GP+H G  ++L A E  
Sbjct: 275 LVDSIKAKLAFLSSLSTKCCIYKVPNKLRRLNPDAYTPRLVSFGPLHRG-KEELQAMEDQ 334

Query: 93  KLQGFINFLRRINNNKIESMEDLLKTGAVNILVEKAHHWVKKARNCYATPTNMMDIDEFV 152
           K +  ++F+ R N+    S+ED         LV  A  W + AR+CYA    +   DEFV
Sbjct: 335 KYRYLLSFIPRTNS----SLED---------LVRLARTWEQNARSCYAEDVKLHS-DEFV 394

Query: 153 MMMLVDACFVVEFLILKFDCDHPDCKFFQIQDNVDISFYQGIDL-HILYDLIKLENQVPF 212
            M++VD  F+VE L+           + +++   D  F   + +  +  D+I +ENQ+PF
Sbjct: 395 EMLVVDGSFLVELLLRS--------HYPRLRGENDRIFGNSMMITDVCRDMILIENQLPF 454

Query: 213 FLLRNLFNLIPKH---DVPMIIFSFGDLTHKALKFRLVRKYEIDLYKEPKHLVDLLSFYF 272
           F+++ +F L+  +     P II     L  +   + L R  +     EP+H VDLL   +
Sbjct: 455 FVVKEIFLLLLNYYQQGTPSII----QLAQRHFSYFLSRIDDEKFITEPEHFVDLLRSCY 514

Query: 273 VPLPLDDMQNKHHGFKNGEEHRWIPPSITELCEAGVTIKKAENAKYVTNITFKNGVLEIP 332
           +P     ++       N        P  TEL  AGV  K AE +  + +I+F +GVL+IP
Sbjct: 515 LPQFPIKLEYTTVKVDNA-------PEATELHTAGVRFKPAETSSCLLDISFADGVLKIP 574

Query: 333 PLHIYDEFELMLRNMVAFEQISAGKKNKYVIQYVLFMDDLISTEKDVRLLVEAGVIINQI 392
            + + D  E + +N++ FEQ     KN   + Y++ +   I +  D  LL+ +G+I+N +
Sbjct: 575 TIVVDDLTESLYKNIIGFEQCRCSNKN--FLDYIMLLGCFIKSPTDADLLIHSGIIVNYL 634

Query: 393 GGSDKEVSDLFNNLCKFITAPRSSHFDPIIKDLCDHCNGRWNKAKASLKHNYFNTPWAFI 452
           G S  +VS+LFN++ K +   R  +F  + ++L  +CN  WN+ KA L+ +YF+ PWA  
Sbjct: 635 GNS-VDVSNLFNSISKEVIYDRRFYFSMLSENLQAYCNTPWNRWKAILRRDYFHNPWAVA 679

Query: 453 SLFAASLLILLTILQTIFSAIS 471
           S+FAA LL+LLT +Q++ S ++
Sbjct: 695 SVFAALLLLLLTFIQSVCSILA 679

BLAST of Bhi06G001351 vs. TAIR 10
Match: AT3G50170.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 178.3 bits (451), Expect = 1.4e-44
Identity = 153/489 (31.29%), Postives = 236/489 (48.26%), Query Frame = 0

Query: 15  THSRVGE--ISEADQRLCGNV-VICIEKMLEQLPQ-----VNAKCSIYRISKELCEVNRK 74
           +HS  G+  + E  +   G+  VI I   LEQ  +     +  K  IYR+   L E ++K
Sbjct: 69  SHSESGKEVVEERPEETTGDSWVISIRDKLEQADRDDDTTIWGKLCIYRVPHYLQENDKK 128

Query: 75  AYTPQLISIGPIHCGTHKDLIANEPYKLQGFINFLRRINNNKIESMEDLLKTGAVNILVE 134
           +Y PQ +S+GP H G  K L   E +K +     L+R+   +IE     + T A+  L E
Sbjct: 129 SYFPQTVSLGPYHHG-KKRLRPMERHKWRALNKVLKRL-KQRIE-----MYTNAMRELEE 188

Query: 135 KAHHWVKKARNCYATPTNMMDIDEFVMMMLVDACFVVEFLILKFDCDHPDCKFFQIQDNV 194
                  KAR CY  P + +  +EF  M+++D CFV+E     F              N 
Sbjct: 189 -------KARACYEGPIS-LSRNEFTEMLVLDGCFVLEL----FRGTVEGFTEIGYARND 248

Query: 195 DISFYQGIDLHILYDLIKLENQVPFFLLRNLFNL---------IPKHDVPMI---IFSFG 254
            +   +G+   I  D+I LENQ+P F+L  L  L         I  H        +   G
Sbjct: 249 PVFAMRGLMHSIQRDMIMLENQLPLFVLDRLLELQLGTQNQTGIVAHVAVKFFDPLMPTG 308

Query: 255 DLTHKALKFRLVRKYE--IDLYKEPKHLVDLLSFYFVPLPLDDMQNKHHGFKN------- 314
           +   K  + +L+   E  +D   +   L  L  F    L      N     K        
Sbjct: 309 EALTKPDQSKLMNWLEKSLDTLGDKGELHCLDVFRRSLLQSSPTPNTRSLLKRLTRNTRV 368

Query: 315 -GEEHRWIPPSITELCEAGVTIKKAENAKYVTNITFKNGVLEIPPLHIYDEFELMLRNMV 374
             +  + +   +TEL EAGV  +K +  ++  +I FKNG LEIP L I+D  + +  N++
Sbjct: 369 VDKRQQQLVHCVTELREAGVKFRKRKTDRF-WDIEFKNGYLEIPKLLIHDGTKSLFSNLI 428

Query: 375 AFEQISAGKKNKYVIQYVLFMDDLISTEKDVRLLVEAGVIINQIGGSDKEVSDLFNNLC- 434
           AFEQ      N ++  Y++FMD+LI++ +DV  L   G+I + + GSD EV+DLFN LC 
Sbjct: 429 AFEQCHIESSN-HITSYIIFMDNLINSSEDVSYLHYCGIIEHWL-GSDSEVADLFNRLCQ 488

Query: 435 KFITAPRSSHFDPIIKDLCDHCNGRWNKAKASLKHNYFNTPWAFISLFAASLLILLTILQ 473
           + +  P+ SH   +  D+  + N +WN  KA+L H YFN PWA+ S  AA +L+LLT+ Q
Sbjct: 489 EVVFDPKDSHLSRLSGDVNRYYNRKWNVLKATLTHKYFNNPWAYFSFSAAVILLLLTLCQ 535

BLAST of Bhi06G001351 vs. TAIR 10
Match: AT3G50130.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 173.7 bits (439), Expect = 3.5e-43
Identity = 146/468 (31.20%), Postives = 231/468 (49.36%), Query Frame = 0

Query: 34  VICIEKMLEQLPQVNA-----KCSIYRISKELCEVNRKAYTPQLISIGPIHCGTHKDLIA 93
           VI I   +EQ  + +A     K  IYR+ + L E N+K+Y PQ +S+GP H G +K L+ 
Sbjct: 117 VISIRDKMEQALREDATTSWDKLCIYRVPQYLQENNKKSYFPQTVSLGPFHHG-NKHLLP 176

Query: 94  NEPYKLQGFINFLRRINNNKIESMEDLLKTGAVNILVEKAHHWVKKARNCYATPTNMMDI 153
            + +K +  +N +     + IE   D +K                +AR CY  P ++   
Sbjct: 177 MDRHKWRA-VNMVMARTKHDIEMYIDAMK------------ELEDRARACYEGPIDLSS- 236

Query: 154 DEFVMMMLVDACFVVEFLILKFDCDHPDCKFFQI-QDNVDISFYQGIDLH-ILYDLIKLE 213
           ++F  M+++D CFV+E           D  F ++  D  D  F     +H I  D++ LE
Sbjct: 237 NKFSEMLVLDGCFVLELF------RGADEGFSELGYDRNDPVFAMRGSMHSIQRDMVMLE 296

Query: 214 NQVPFFLLRNL--FNLIPKHDVPMI----IFSFGDL---------THKALKFRLVRKYEI 273
           NQ+P F+L  L    L  +H   ++    +  F  L         T  +L+         
Sbjct: 297 NQLPLFVLNRLLEIQLGKRHQTGLVSRLAVRFFDPLMPTDEPLTKTDDSLEQDKFFNPIA 356

Query: 274 DLYKEPKHLVD-----LLSFYFVPLP-LDDMQNKHHGFKNGEEHRWIPPSITELCEAGVT 333
           D  K   H +D     LL     P P L  M+         +  + +   +TEL EAG+ 
Sbjct: 357 DKDKGELHCLDVFRRNLLRPCSNPEPRLSRMRWSWRTRVADKRQQQLIHCVTELREAGIK 416

Query: 334 IKKAENAKYVTNITFKNGVLEIPPLHIYDEFELMLRNMVAFEQISAGKKNKYVIQYVLFM 393
            +  +  ++  +I FKNG LEIP L I+D  + +  N++AFEQ      N  +  Y++FM
Sbjct: 417 FRTRKTDRF-WDIRFKNGYLEIPKLLIHDGTKSLFSNLIAFEQCHIDSSND-ITSYIIFM 476

Query: 394 DDLISTEKDVRLLVEAGVIINQIGGSDKEVSDLFNNLCKFIT-APRSSHFDPIIKDLCDH 453
           D+LI + +DVR L   G+I + + G+D EV+DLFN LC+ +   P++S+   +   +  +
Sbjct: 477 DNLIDSSEDVRYLHYCGIIEHWL-GNDYEVADLFNRLCQEVAFDPQNSYLSQLSNKVDRN 536

Query: 454 CNGRWNKAKASLKHNYFNTPWAFISLFAASLLILLTILQTIFSAISAF 473
            + +WN  KA LKH YFN PWA+ S FAA +L++LT+ Q+ F+A   F
Sbjct: 537 YSRKWNVLKAILKHKYFNNPWAYFSFFAALVLLVLTLFQSFFTAYPYF 560

BLAST of Bhi06G001351 vs. TAIR 10
Match: AT3G50160.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 173.3 bits (438), Expect = 4.6e-43
Identity = 132/421 (31.35%), Postives = 209/421 (49.64%), Query Frame = 0

Query: 53  IYRISKELCEVNRKAYTPQLISIGPIHCGTHKDLIANEPYKLQGFINFLRRINNNKIESM 112
           IYR+   L E + K+Y PQ++SIGP H G HK L+  E +K +  +N +     + IE  
Sbjct: 106 IYRVPPYLQENDTKSYMPQIVSIGPYHHG-HKHLMPMERHKWRA-VNMVMARAKHDIEMY 165

Query: 113 EDLLKTGAVNILVEKAHHWVKKARNCYATPTNMMDIDEFVMMMLVDACFVVEFLILKFDC 172
            D +K               +KAR CY  P N M+ +EF+ M+++D  F++E     F  
Sbjct: 166 IDAMK------------ELEEKARACYQGPIN-MNRNEFIEMLVLDGVFIIEI----FKG 225

Query: 173 DHPDCKFFQIQDNVDISFYQGIDLHILYDLIKLENQVPFFLLRNLFNLIPKHDVPMIIFS 232
                +      N  +   +G+   I  D++ LENQ+P+ +L+ L  L      P ++  
Sbjct: 226 TSEGFQEIGYAPNDPVFGMRGLMQSIRRDMVMLENQLPWSVLKGLLQL----QRPDVLDK 285

Query: 233 FGDLTHKALKFRLVRKYEIDLYKEPKHLVDLLSFYFVPLPLDDMQNKHHGFKNGEEHRWI 292
                 +     L+   E+   +   H +D+L      L      ++     N +  + I
Sbjct: 286 VNVQLFQPFFQPLLPTREVLTEEGGLHCLDVLRRGL--LQSSGTSDEDMSMVNKQPQQLI 345

Query: 293 PPSITELCEAGVTIKKAENAKYVTNITFKNGVLEIPPLHIYDEFELMLRNMVAFEQISAG 352
              +TEL  AGV   + E   +  +I FKNG L+IP L I+D  + +  N++AFEQ    
Sbjct: 346 -HCVTELRNAGVEFMRKETGHF-WDIEFKNGYLKIPKLLIHDGTKSLFLNLIAFEQCHI- 405

Query: 353 KKNKYVIQYVLFMDDLISTEKDVRLLVEAGVIINQIGGSDKEVSDLFNNLCK-FITAPRS 412
           K +K +  Y++FMD+LI++ +DV  L   G+I N + GSD EVSDLFN L K  I  P  
Sbjct: 406 KSSKKITSYIIFMDNLINSSEDVSYLHHYGIIENWL-GSDSEVSDLFNGLGKEVIFDPND 465

Query: 413 SHFDPIIKDLCDHCNGRWNKAKASLKHNYFNTPWAFISLFAASLLILLTILQTIFSAISA 472
            +   +  ++  +   +WN  KA+L+H YFN PWA+ S  AA  L++ T  Q+ F+  + 
Sbjct: 466 GYLSALTGEVNIYYRRKWNYLKATLRHKYFNNPWAYFSFIAAVTLLIFTFCQSFFAVFAY 497

BLAST of Bhi06G001351 vs. TAIR 10
Match: AT3G50150.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 171.4 bits (433), Expect = 1.7e-42
Identity = 139/466 (29.83%), Postives = 227/466 (48.71%), Query Frame = 0

Query: 34  VICIEKMLEQLPQVNA-----KCSIYRISKELCEVNRKAYTPQLISIGPIHCGTHKDLIA 93
           VI I+  +E+    +A     K  IYR+   L E ++K+Y PQ +SIGP H G    L  
Sbjct: 68  VISIKDKMEKALSYDATNSWDKLCIYRVPFYLQENDKKSYLPQTVSIGPYHHG-KVHLRP 127

Query: 94  NEPYKLQGFINFLRRINNNKIESMEDLLKTGAVNILVEKAHHWVKKARNCYATPTNMMDI 153
            E +K +  +N +     + IE   D +K               ++AR CY  P +M + 
Sbjct: 128 MERHKWRA-VNMIMARTKHNIEMYIDAMK------------ELEEEARACYQGPIDMKNS 187

Query: 154 DEFVMMMLVDACFVVEFLILKFDCDHPDCKFFQIQDNVDISFYQGIDLHILYDLIKLENQ 213
           +EF  M+++D CFV+E     F       +      N  +   +G+   I  D+I LENQ
Sbjct: 188 NEFTEMLVLDGCFVLEL----FKGTIQGFQKIGYARNDPVFAKRGLMHSIQRDMIMLENQ 247

Query: 214 VPFFLLRNLFNLIPKHDVPMIIFSFGDLTHKALKF--------RLVRKYEIDLYKEPK-- 273
           +P F+L  L  L  +   P      G +   A++F         ++ K E  L  + K  
Sbjct: 248 LPLFVLDRLLGL--QTGTPN---QTGIVAEVAVRFFKTLMPTSEVLTKSERSLDSQEKSD 307

Query: 274 --------HLVDLLSFYFVPLPLDDMQNK---HHGFKNGEEHRWIPPSITELCEAGVTIK 333
                   H +D+  F+   +   +  N+   +      E+ + +   +TEL  AGV   
Sbjct: 308 ELGDNGGLHCLDV--FHRSLIQSSETTNQGTPYEDMSMVEKQQQLIHCVTELRGAGVNFM 367

Query: 334 KAENAKYVTNITFKNGVLEIPPLHIYDEFELMLRNMVAFEQISAGKKNKYVIQYVLFMDD 393
           + E  + + +I FKNG L+IP L I+D  + +  N++AFEQ      N  +  Y++FMD+
Sbjct: 368 RKETGQ-LWDIEFKNGYLKIPKLLIHDGTKSLFSNLIAFEQCHTQSSNN-ITSYIIFMDN 427

Query: 394 LISTEKDVRLLVEAGVIINQIGGSDKEVSDLFNNLCK-FITAPRSSHFDPIIKDLCDHCN 453
           LI++ +DV  L   G+I + + GSD EV+DLFN LCK  I  P+  +   + +++  + +
Sbjct: 428 LINSSQDVSYLHHDGIIEHWL-GSDSEVADLFNRLCKEVIFDPKDGYLSQLSREVNRYYS 487

Query: 454 GRWNKAKASLKHNYFNTPWAFISLFAASLLILLTILQTIFSAISAF 473
            +WN  KA+L+  YFN PWA+ S  AA +L+ LT  Q+ F+  + +
Sbjct: 488 RKWNSLKATLRQKYFNNPWAYFSFSAAVILLFLTFFQSFFAVYAYY 505

BLAST of Bhi06G001351 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 3.7e-29
Identity = 119/443 (26.86%), Postives = 203/443 (45.82%), Query Frame = 0

Query: 51  CSIYRISKELCEVNRKAYTPQLISIGPIHCG-THKDLI-ANEPYKLQGFINFLRRINNNK 110
           C I+R+ +    +N KAY P+++SIGP H G  H  +I  ++P  LQ F++  ++     
Sbjct: 46  CCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAKK----- 105

Query: 111 IESMEDLLKTGAVNILVEKAHHWVKKARNCYATPTNMMDIDEFVMMMLVDACFV-VEFLI 170
                   K    N+LV+       K R  Y+    +    + + MM++D CF+ + FLI
Sbjct: 106 --------KDVEENVLVKAVVDLEDKIRKSYS--EELKTGHDLMFMMVLDGCFILMVFLI 165

Query: 171 LKFDCDHPDCKFFQIQDNVDISFYQGIDLHILYDLIKLENQVPFFLLRNLF---NLIPKH 230
           +  + +  +   F I           +   I  DL+ LENQVPFF+L+ L+    +    
Sbjct: 166 MSGNIELSEDPIFSI---------PWLLSSIQSDLLLLENQVPFFVLQTLYVGSKIGVSS 225

Query: 231 DVPMIIFSF--GDLTHKALKFRLVRKYEIDLYKEPKHLVDLLSFYFVPLPLDD------- 290
           D+  I F F    +  +   +   R Y      + KHL+DL+   F+P   +        
Sbjct: 226 DLNRIAFHFFKNPIDKEGSYWEKHRNY------KAKHLLDLIRETFLPNTSESDKASSPH 285

Query: 291 MQNKHHGFKNGE----EHRWIP--PSITELCEAGVTIK-KAENAKYVTNITFKNGVLEIP 350
           +Q + H  K+G     + + +P   S   L   G+  + +      + N+  K   L+IP
Sbjct: 286 VQVQLHEGKSGNVPSVDSKAVPLILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNKLQIP 345

Query: 351 PLHIYDEFELMLRNMVAFEQISAGKKNKYVIQYVLFMDDLISTEKDVRLLVEAGVIINQI 410
            L           N VAFEQ      N+ +  Y++FM  L++ E+DV  L    +II   
Sbjct: 346 QLRFDGFISSFFLNCVAFEQFYTDSSNE-ITTYIVFMGCLLNNEEDVTFLRNDKLIIENH 405

Query: 411 GGSDKEVSDLFNNLCK-FITAPRSSHFDPIIKDLCDHCNGRWNKAKASLKHNYFNTPWAF 470
            GS+ EVS+ F  + K  +    +S+ + + K + ++    +N   A  +H +F +PW F
Sbjct: 406 FGSNNEVSEFFKTISKDVVFEVDTSYLNNVFKGVNEYTKKWYNGLWAGFRHTHFESPWTF 457

BLAST of Bhi06G001351 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 2.2e-21
Identity = 129/530 (24.34%), Postives = 213/530 (40.19%), Query Frame = 0

Query: 32  NVVICIEKMLEQLPQVNAKCSIYRISKELCEVNRKAYTPQLISIGPIHCGTHKDLIANEP 91
           NV   ++  LE+        SI+ + K L   +  +YTP  +SIGP HC    +L   E 
Sbjct: 24  NVQKSLDAELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHC-LKPELHEMER 83

Query: 92  YKLQGFINFLRRINNNKIESMEDLLKTGAVNILVEKAHHWVKKARNCYATPTNMMDIDEF 151
           YKL       R+I N           +   + LVEK      K R CY       + +  
Sbjct: 84  YKLM----IARKIRNQ--------YNSFRFHDLVEKLQSMEIKIRACYHKYIG-FNGETL 143

Query: 152 VMMMLVDACFVVEFLILKFDCDHPDCKFFQIQDNVDISFYQGIDLHILYDLIKLENQVPF 211
           + +M VD+ F++EFL           K +  +  V+    +     IL D++ +ENQ+P 
Sbjct: 144 LWIMAVDSSFLIEFL-----------KIYSFR-KVETLINRVGHNEILRDIMMIENQIPL 203

Query: 212 FLLRNL--FNLIPKHDVPMIIFSFGDLTHKALKFRLVRKYEID-----LYKEPKHLVDLL 271
           F+LR    F L        ++ S      K L   LV K++ D      ++E  H++D L
Sbjct: 204 FVLRKTLEFQLESTESADDLLLSVLTGLCKDLS-PLVIKFDDDQILKAQFQECNHILDFL 263

Query: 272 SFYFVP------LPLDDMQNK----------------HHGFKNGEEHR---------W-- 331
               VP      L  DD +N+                 H FK     R         W  
Sbjct: 264 YQMIVPRIEEEELEEDDEENRADENGGNRAIRFMDEIKHQFKRVFASRPADLILRFPWRI 323

Query: 332 ---IP--------------------------------------------PSITELCEAGV 391
              +P                                            PS+++L +AGV
Sbjct: 324 ISNLPGFMALKLSADYLFTRQENEATTTRQESVSILDIEKPPLVEELTIPSVSDLHKAGV 383

Query: 392 TIKKAENAKYVTNITF--KNGVLEIPPLHIYDEFELMLRNMVAFEQISAGKKNKYVIQYV 451
             K   +   ++ +TF   +G   +P +++    E +LRN+VA+E  +      +  +Y 
Sbjct: 384 RFKPTAHGN-ISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEATNTSGPLVFT-RYT 443

Query: 452 LFMDDLISTEKDVRLLVEAGVIINQIGGSDKEVSDLFNNLCKFITAPRSSHFDPIIKDLC 473
             ++ +I +E+DVRLL E GV+++++  SD+E ++++N + K +   +    D  I+D+ 
Sbjct: 444 ELINGIIDSEEDVRLLREQGVLVSRL-KSDQEAAEMWNGMSKSVRLTKVGFLDKTIEDVN 503

BLAST of Bhi06G001351 vs. ExPASy TrEMBL
Match: A0A5A7V9V0 (UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002610 PE=4 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 1.8e-204
Identity = 362/460 (78.70%), Postives = 410/460 (89.13%), Query Frame = 0

Query: 19  VGEISEA-DQRLCGNVVICIEKMLEQLPQVNAKCSIYRISKELCEVNRKAYTPQLISIGP 78
           + EISE  DQ+LCGNVVICI KML+QLPQVNA+CSIY+ISKEL E+NRKAY PQLISIGP
Sbjct: 1   MAEISEVDDQKLCGNVVICIGKMLKQLPQVNAECSIYQISKELLEINRKAYIPQLISIGP 60

Query: 79  IHCGTHKDLIANEPYKLQGFINFLRR--INNNKIESMEDLLKTGAVNILVEKAHHWVKKA 138
           IH GT+ DL+AN+ YKLQGFINFLRR  INN +I SMED+L+TG +N LVEKAHHWVK+A
Sbjct: 61  IHHGTNNDLVANQQYKLQGFINFLRRININNKQILSMEDILQTGTLNTLVEKAHHWVKEA 120

Query: 139 RNCYAT-PTNMMDIDEFVMMMLVDACFVVEFLILKFDCDHPDCKFFQIQDNVDISFYQGI 198
           RNCY + P N +D+D FV+MMLVDACF+VEFLILKFD DHP+ KF QIQDN+DISFYQG+
Sbjct: 121 RNCYTSPPINTIDMDAFVIMMLVDACFIVEFLILKFDYDHPNGKFLQIQDNIDISFYQGM 180

Query: 199 DLHILYDLIKLENQVPFFLLRNLFNLIPKHDVPMIIFSFGDLTHKALKFRLVRKYEIDLY 258
           DLHILYDLIKLENQVPFFLL+ LF+LIPKHD+ M+I SF DLT +ALKFRLVR YEI+L+
Sbjct: 181 DLHILYDLIKLENQVPFFLLQYLFDLIPKHDISMMISSFRDLTLRALKFRLVRTYEINLF 240

Query: 259 KEPKHLVDLLSFYFVPLPLDDMQNKHHGFKN--GEEHRWIPPSITELCEAGVTIKKAENA 318
           KEPKH VDLL+FYFVP     + N+H  FK+   E++RWIPPSITEL EAGVTIKKAE A
Sbjct: 241 KEPKHFVDLLTFYFVPSAGQKVNNQHGIFKSTIEEKNRWIPPSITELREAGVTIKKAEKA 300

Query: 319 KYVTNITFKNGVLEIPPLHIYDEFELMLRNMVAFEQISAGKKNKYVIQYVLFMDDLISTE 378
           K++T+ITFKNGVL IPPLHIYDEFEL+LRNMVAFEQISA K NKYVIQYVLFMDDLISTE
Sbjct: 301 KHLTDITFKNGVLRIPPLHIYDEFELVLRNMVAFEQISAKKMNKYVIQYVLFMDDLISTE 360

Query: 379 KDVRLLVEAGVIINQIGGSDKEVSDLFNNLCKFITAPRSSHFDPIIKDLCDHCNGRWNKA 438
           KDVRLLVEAGVIINQIGGSDKEV+DLFNN CKFITAP+SS+FD IIKDLC+HCNGRWN+A
Sbjct: 361 KDVRLLVEAGVIINQIGGSDKEVADLFNNFCKFITAPKSSNFDSIIKDLCEHCNGRWNRA 420

Query: 439 KASLKHNYFNTPWAFISLFAASLLILLTILQTIFSAISAF 473
           KASLKHNYFNTPWAFIS FAA+LLILLT+LQTIF+AI+ F
Sbjct: 421 KASLKHNYFNTPWAFISFFAATLLILLTLLQTIFTAITTF 460

BLAST of Bhi06G001351 vs. ExPASy TrEMBL
Match: A0A1S3BCY5 (UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103488292 PE=4 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 6.8e-204
Identity = 360/460 (78.26%), Postives = 410/460 (89.13%), Query Frame = 0

Query: 19  VGEISEA-DQRLCGNVVICIEKMLEQLPQVNAKCSIYRISKELCEVNRKAYTPQLISIGP 78
           + EISE  DQ+LCGNVVICI KML+QLPQVNA+CSIY++SKEL E+NRKAY PQLISIGP
Sbjct: 1   MAEISEVDDQKLCGNVVICIGKMLKQLPQVNAECSIYQVSKELLEINRKAYIPQLISIGP 60

Query: 79  IHCGTHKDLIANEPYKLQGFINFLRR--INNNKIESMEDLLKTGAVNILVEKAHHWVKKA 138
           IH GT+ DL+AN+ YKLQGFINFLRR  INN +I SMED+L+TG +N LVEKAHHWV++A
Sbjct: 61  IHHGTNNDLVANQQYKLQGFINFLRRININNKQILSMEDILQTGTLNTLVEKAHHWVEEA 120

Query: 139 RNCYAT-PTNMMDIDEFVMMMLVDACFVVEFLILKFDCDHPDCKFFQIQDNVDISFYQGI 198
           RNCY + P N +D+D FV+MMLVDACF+VEFLILKFD DHP+ KF QIQDN+DISFYQG+
Sbjct: 121 RNCYTSPPINTIDMDAFVIMMLVDACFIVEFLILKFDYDHPNGKFLQIQDNIDISFYQGM 180

Query: 199 DLHILYDLIKLENQVPFFLLRNLFNLIPKHDVPMIIFSFGDLTHKALKFRLVRKYEIDLY 258
           DLHILYDLIKLENQVPFFLL+ LF+LIPKHD+ M+I SF DLT +ALKFRLVR YEI+L+
Sbjct: 181 DLHILYDLIKLENQVPFFLLQYLFDLIPKHDISMMISSFRDLTLRALKFRLVRTYEINLF 240

Query: 259 KEPKHLVDLLSFYFVPLPLDDMQNKHHGFKN--GEEHRWIPPSITELCEAGVTIKKAENA 318
           KEPKH VDLL+FYFVP     + N+H  FK+   E++RWIPPSITEL EAGVTIKKAE A
Sbjct: 241 KEPKHFVDLLTFYFVPSAGQKVNNQHGIFKSTIEEKNRWIPPSITELREAGVTIKKAEKA 300

Query: 319 KYVTNITFKNGVLEIPPLHIYDEFELMLRNMVAFEQISAGKKNKYVIQYVLFMDDLISTE 378
           K++T+ITFKNGVL IPPLHIYDEFEL+LRNMVAFEQISA K NKYVIQYVLFMDDLISTE
Sbjct: 301 KHLTDITFKNGVLRIPPLHIYDEFELVLRNMVAFEQISARKMNKYVIQYVLFMDDLISTE 360

Query: 379 KDVRLLVEAGVIINQIGGSDKEVSDLFNNLCKFITAPRSSHFDPIIKDLCDHCNGRWNKA 438
           KDVRLLVEAGVIINQIGGSDKEV+DLFNN CKFITAP+SS+FD IIKDLC+HCNGRWN+A
Sbjct: 361 KDVRLLVEAGVIINQIGGSDKEVADLFNNFCKFITAPKSSNFDSIIKDLCEHCNGRWNRA 420

Query: 439 KASLKHNYFNTPWAFISLFAASLLILLTILQTIFSAISAF 473
           KASLKHNYFNTPWAFIS FAA+LLILLT+LQTIF+AI+ F
Sbjct: 421 KASLKHNYFNTPWAFISFFAATLLILLTLLQTIFTAITTF 460

BLAST of Bhi06G001351 vs. ExPASy TrEMBL
Match: A0A5A7VF39 (UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002650 PE=4 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 2.1e-144
Identity = 291/521 (55.85%), Postives = 349/521 (66.99%), Query Frame = 0

Query: 6   IEKQEANDITHSRVGEISEADQRLCGNVVICIEKMLEQLPQVNAKCSIYRISKELCEVNR 65
           + K EA  ++++ + +I EA Q LC NVVI I+KML Q+  +N  CSIYRI K+L E+N 
Sbjct: 19  MHKVEAESVSNNEMPQIIEAQQDLCDNVVISIQKMLNQMHSINGDCSIYRIPKQLREMNP 78

Query: 66  KAYTPQLISIGPI-HCGTHKDLIANEPYKLQGFINFLRRINNN-----------KIESME 125
           KAYTPQLISIGP  H     D  A E YKLQ  +NFLRRINNN           K  S++
Sbjct: 79  KAYTPQLISIGPFHHYRRQNDFKATEQYKLQALVNFLRRINNNNETMEEDQDVKKSRSLD 138

Query: 126 DLLKTGAVNILVEKAHHWVKKARNCYATPTNMMDIDEFVMMMLVDACFVVEFLILKFDCD 185
           DLLK G + +LVEK H W+ + RNCY+ P + MD   FV+MML+DACF+VE  I ++D  
Sbjct: 139 DLLKIGTLKVLVEKVHCWMTETRNCYSEPIDYMDDHNFVIMMLLDACFIVESFIQQYDAS 198

Query: 186 HP-DCKFFQIQDNVD-ISFYQGIDLHILYDLIKLENQVPFFLLRNLFNLIPKHDVPMIIF 245
           +P D KF +IQDNVD +  Y      I  DLIKLENQVPFFLL+++F++IP+H  P+   
Sbjct: 199 YPHDRKFPRIQDNVDFLLLYPETSQDISNDLIKLENQVPFFLLQHMFHMIPQHSPPV--- 258

Query: 246 SFGDLTHKALKFRLVRKYEI----DLYKEPKHLVDLLSFYFVPL-PLDDMQNKHHGFKNG 305
           SF +LT+ AL    +  Y+I     +  EPKHLV  LSFYF+P    DDM       KN 
Sbjct: 259 SFINLTYSALYDWFISTYKICSVGIVPNEPKHLVHFLSFYFIPSHTTDDMIQNKQEIKNS 318

Query: 306 EEHR------------------W--------------IPPSITELCEAGVTIKKAENAKY 365
           E  +                  W               PPSITELCE+GVTI+KA+NAKY
Sbjct: 319 ENKKKNNYNFLSFFSTLFCCQLWQNPDNKKKNDQELLSPPSITELCESGVTIEKAKNAKY 378

Query: 366 VTNITFKNGVLEIPPLHIYDEFELMLRNMVAFEQISAGKKNKYVIQYVLFMDDLISTEKD 425
           +TNITFKNGVL+IP LHIYDEFELM RN++AFEQ  AG +N Y  QY+LFMDDLISTEKD
Sbjct: 379 LTNITFKNGVLKIPHLHIYDEFELMFRNLIAFEQFPAGNENMYATQYILFMDDLISTEKD 438

Query: 426 VRLLVEAGVIINQIGGSDKEVSDLFNNLCKFIT-APRSSHFDPIIKDLCDHCNGRWNKAK 475
           VRLLV +GVIIN IGGSDKEVS+LFNNL KF+   P    F+ I K L  HCNGRWNKAK
Sbjct: 439 VRLLVNSGVIINNIGGSDKEVSELFNNLGKFVNQCPSPYMFNHISKALRKHCNGRWNKAK 498

BLAST of Bhi06G001351 vs. ExPASy TrEMBL
Match: A0A1S3BD29 (LOW QUALITY PROTEIN: UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103488563 PE=4 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 1.4e-143
Identity = 289/520 (55.58%), Postives = 348/520 (66.92%), Query Frame = 0

Query: 6   IEKQEANDITHSRVGEISEADQRLCGNVVICIEKMLEQLPQVNAKCSIYRISKELCEVNR 65
           + K EA  ++++ + +I EA Q LC NVVI I+KML Q+  +N  CSIYRI K+L E+N 
Sbjct: 19  MHKVEAESVSNNEMPQIIEAQQDLCDNVVISIQKMLNQMHSINGDCSIYRIPKQLREMNP 78

Query: 66  KAYTPQLISIGPI-HCGTHKDLIANEPYKLQGFINFLRRINNN-----------KIESME 125
           KAYTPQLISIGP  H     D  A E YKLQ  +NFLRRINNN           K  S++
Sbjct: 79  KAYTPQLISIGPFHHYRRQNDFKATEQYKLQALVNFLRRINNNNETMEEDQDVKKSRSLD 138

Query: 126 DLLKTGAVNILVEKAHHWVKKARNCYATPTNMMDIDEFVMMMLVDACFVVEFLILKFDCD 185
           DLLK G + +LVEK H W+ + RNCY+ P + MD   FV+MML+DACF+VE  I ++D  
Sbjct: 139 DLLKIGTLKVLVEKVHCWMTETRNCYSEPIDYMDDHNFVIMMLLDACFIVESFIQQYDAS 198

Query: 186 HP-DCKFFQIQDNVD-ISFYQGIDLHILYDLIKLENQVPFFLLRNLFNLIPKHDVPMIIF 245
           +P D KF +IQDNVD +  Y      I  DLIKLENQVPFFLL+++F++IP+H  P+   
Sbjct: 199 YPHDRKFPRIQDNVDFLLLYPETSQDISNDLIKLENQVPFFLLQHMFHMIPQHSPPV--- 258

Query: 246 SFGDLTHKALKFRLVRKYEI----DLYKEPKHLVDLLSFYFVPL-PLDDMQNKHHGFKNG 305
           SF +LT+ AL    +  Y+I     +  EPKHLV  LSFYF+P    DDM       KN 
Sbjct: 259 SFINLTYSALYDWFISTYKICSVGIVPNEPKHLVHFLSFYFIPSHTTDDMIQNKQEIKNS 318

Query: 306 EEHR-------------------------------WIPPSITELCEAGVTIKKAENAKYV 365
           E  +                               +  PSITELCE+GVTI+KA+NAKY+
Sbjct: 319 ENKKKNNYNFLSFFSTLFCCQLWQNPDNKKKMIRNFCLPSITELCESGVTIEKAKNAKYL 378

Query: 366 TNITFKNGVLEIPPLHIYDEFELMLRNMVAFEQISAGKKNKYVIQYVLFMDDLISTEKDV 425
           TNITFKNGVL+IP LHIYDEFELM RN++AFEQ  AG +N Y  QY+LFMDDLISTEKDV
Sbjct: 379 TNITFKNGVLKIPHLHIYDEFELMFRNLIAFEQFPAGNENMYATQYILFMDDLISTEKDV 438

Query: 426 RLLVEAGVIINQIGGSDKEVSDLFNNLCKFIT-APRSSHFDPIIKDLCDHCNGRWNKAKA 475
           RLLV +GVIIN IGGSDKEVS+LFNNL KF+   P    F+ I K L  HCNGRWNKAKA
Sbjct: 439 RLLVNSGVIINNIGGSDKEVSELFNNLGKFVNQCPSPYMFNHISKALRKHCNGRWNKAKA 498

BLAST of Bhi06G001351 vs. ExPASy TrEMBL
Match: A0A5A7VBG0 (UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002670 PE=4 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 7.0e-140
Identity = 283/510 (55.49%), Postives = 352/510 (69.02%), Query Frame = 0

Query: 6   IEKQEANDITHSRVGEISEADQRLCGNVVICIEKMLEQLPQVNAKCSIYRISKELCEVNR 65
           + + EA +++++ + +ISEA Q LC NVVI IEKML Q+  +N  CSIYR+ K+L ++N 
Sbjct: 71  VHEVEAKNVSNNDMPQISEAQQDLCDNVVISIEKMLNQMHSINEDCSIYRVPKQLRKMNP 130

Query: 66  KAYTPQLISIGPI-HCGTHKDLIANEPYKLQGFINFLRRINNNK-----------IESME 125
           + YTPQLISIGP  H     D  A E YKLQ  +NFLRRINN+            I S+E
Sbjct: 131 EVYTPQLISIGPFHHYRCQNDFKATEQYKLQALVNFLRRINNDNEIMKEANVVKIIRSLE 190

Query: 126 DLLKTGAVNILVEKAHHWVKKARNCYATPTNMMDIDEFVMMMLVDACFVVEFLILKFDCD 185
           DLLKT  + +LV+K   W+ +ARN Y+ P + MD   FV+MML+DACF+VE  I K+D  
Sbjct: 191 DLLKTETLKVLVKKVQCWMTEARNYYSEPIDYMDDHNFVIMMLMDACFIVELFIQKYDFS 250

Query: 186 HP-DCKFFQIQDNVD-ISFYQGIDLHILYDLIKLENQVPFFLLRNLFNLIPKHDVPMIIF 245
            P D KF QI+DN++ +  Y  I  +I  DLIKLENQVPFFLL+N+F +IP+H  P+   
Sbjct: 251 CPCDPKFHQIKDNINLLLLYPEITTYITNDLIKLENQVPFFLLQNMFYMIPQHSPPV--- 310

Query: 246 SFGDLTHKALKFRLVRKYE---IDLY-KEPKHLVDLLSFYFVPLP-LDDM---------- 305
           SF +LT+ AL    +  Y+   +D+   EPKHLV  LSFYF+P P  DDM          
Sbjct: 311 SFINLTYIALHDWFMSTYKTCSVDIVPNEPKHLVHFLSFYFIPSPTTDDMIQNNPNFLSF 370

Query: 306 ----------QNKHHGFKNGEEHRWIPPSITELCEAGVTIKKAENAKYVTNITFKNGVLE 365
                     QN+     +  E    PPSITEL E+GVTIKKA+NAKY+TNITFKNGVLE
Sbjct: 371 FSTLFCCQLWQNRPDKKDSDRELLSRPPSITELRESGVTIKKAKNAKYLTNITFKNGVLE 430

Query: 366 IPPLHIYDEFELMLRNMVAFEQISAGKKNKYVIQYVLFMDDLISTEKDVRLLVEAGVIIN 425
           IP LHIYDEFEL++RN++AFEQ+ A  +N Y  QY+LFMDD+ISTEKDVR+LV + VI+N
Sbjct: 431 IPHLHIYDEFELIIRNVIAFEQVHARDRNMYATQYILFMDDMISTEKDVRILVNSEVIVN 490

Query: 426 QIGGSDKEVSDLFNNLCKFITA--PRSSHFDPIIKDLCDHCNGRWNKAKASLKHNYFNTP 475
            IGGSDKEV++LFNNL KFI +    SS F+ I K L  HCNGRWNKAKASLKHNYFNTP
Sbjct: 491 NIGGSDKEVTELFNNLAKFINSFNINSSKFNHISKALHKHCNGRWNKAKASLKHNYFNTP 550

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G31980.11.4e-5532.58unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247,... [more]
AT3G50170.11.4e-4431.29Plant protein of unknown function (DUF247) [more]
AT3G50130.13.5e-4331.20Plant protein of unknown function (DUF247) [more]
AT3G50160.14.6e-4331.35Plant protein of unknown function (DUF247) [more]
AT3G50150.11.7e-4229.83Plant protein of unknown function (DUF247) [more]
Match NameE-valueIdentityDescription
Q9SD533.7e-2926.86UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1[more]
P0C8972.2e-2124.34Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 ... [more]
Match NameE-valueIdentityDescription
A0A5A7V9V01.8e-20478.70UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00261... [more]
A0A1S3BCY56.8e-20478.26UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103488292 PE=4 SV=1[more]
A0A5A7VF392.1e-14455.85UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00265... [more]
A0A1S3BD291.4e-14355.58LOW QUALITY PROTEIN: UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=L... [more]
A0A5A7VBG07.0e-14055.49UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00267... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 53..459
e-value: 3.0E-99
score: 333.0
NoneNo IPR availablePANTHERPTHR31170BNAC04G53230D PROTEINcoord: 31..470
NoneNo IPR availablePANTHERPTHR31170:SF13BNAC04G53230D PROTEINcoord: 31..470

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi06M001351Bhi06M001351mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane