Bhi06G001165 (gene) Wax gourd (B227) v1

Overview
NameBhi06G001165
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionReverse transcriptase
Locationchr6: 39379513 .. 39380302 (-)
RNA-Seq ExpressionBhi06G001165
SyntenyBhi06G001165
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAAATTCTGGTGGGACTCCCACACAAAGAACAATCAAAGGTACAGTCTCATGGCAACAGTTCAATCAAGCTTTTTACAACAAGTATTTCCCTTTGACAGTGAGATATCAAAAGGAAGTGGAATTTCTTAATCTTCGTCAAGAGAATATGTTAGTGGCAGAATATGAACGGAAATTTGATCGCTTGTCTCACTTTGTTCCTCGACTAGTGGACACAGAAGAAAAGAAGGTGGAAAGGTTTATATGGGGATTAAGAGAAGGCATTCGAGCCATTGTTACTGCTTTCCGACACAAAGAGTATTGCTCTTGCCCTCGAGTCGGCCTTACTCATGGAAGTAGATCTTGTTGCCAAAACCTCAACCTCGGAACGAGGATTCATGCCAAATCAAAAGAGGAAACTCACCCAACAAGGCTTCCAACGTTCACAACGGCAGAAAAAAATTTCCCAAAACACTCAGAAGCATACAGTCACAACAGAGAAGCCGAACATTTACATATTCTAAGTGTAGAAATTGTGGAAAAACACCATCAGGGGCAATGTTTTCAGAACATAAGGGGTGTTTCAACTACGGCCAGACGGGTCACTATGCAAGGGCTTGCCCAAAGTTCATCAACCAAGTATCAGCTACCCAGACCACTCAGCCTACTAGTAACAAGGTTACTCAACAACAGAAAGGGACGGGTGTTTGCTGTTACACGTCAAGAGGCAGAAAATCAAGATGCAGTGGTTACAGGTACATTACCCATCCGCGAGTATTATGACTTCACTTTATTTGATTCGGGCTCTA

mRNA sequence

ATGCAAAATTCTGGTGGGACTCCCACACAAAGAACAATCAAAGGTACAGTCTCATGGCAACAGTTCAATCAAGCTTTTTACAACAAGTATTTCCCTTTGACAGTGAGATATCAAAAGGAAGTGGAATTTCTTAATCTTCGTCAAGAGAATATGTTAGTGGCAGAATATGAACGGAAATTTGATCGCTTGTCTCACTTTGTTCCTCGACTAGTGGACACAGAAGAAAAGAAGGTGGAAAGGTTTATATGGGGATTAAGAGAAGGCATTCGAGCCATTGTTACTGCTTTCCGACACAAAGAGTATTGCTCTTGCCCTCGAGTCGGCCTTACTCATGGAAGTAGATCTTGTTGCCAAAACCTCAACCTCGGAACGAGGATTCATGCCAAATCAAAAGAGGAAACTCACCCAACAAGGCTTCCAACGTTCACAACGGCAGAAAAAAATTTCCCAAAACACTCAGAAGCATACAGTCACAACAGAGAAGCCGAACATTTACATATTCTAAGTGTAGAAATTGTGGAAAAACACCATCAGGGGCAATGTTTTCAGAACATAAGGGGTGTTTCAACTACGGCCAGACGGGTCACTATGCAAGGGCTTGCCCAAAGTTCATCAACCAAGTATCAGCTACCCAGACCACTCAGCCTACTAGTAACAAGGTTACTCAACAACAGAAAGGGACGGGTGTTTGCTGTTACACGTCAAGAGGCAGAAAATCAAGATGCAGTGGTTACAGGTACATTACCCATCCGCGAGTATTATGACTTCACTTTATTTGATTCGGGCTCTA

Coding sequence (CDS)

ATGCAAAATTCTGGTGGGACTCCCACACAAAGAACAATCAAAGGTACAGTCTCATGGCAACAGTTCAATCAAGCTTTTTACAACAAGTATTTCCCTTTGACAGTGAGATATCAAAAGGAAGTGGAATTTCTTAATCTTCGTCAAGAGAATATGTTAGTGGCAGAATATGAACGGAAATTTGATCGCTTGTCTCACTTTGTTCCTCGACTAGTGGACACAGAAGAAAAGAAGGTGGAAAGGTTTATATGGGGATTAAGAGAAGGCATTCGAGCCATTGTTACTGCTTTCCGACACAAAGAGTATTGCTCTTGCCCTCGAGTCGGCCTTACTCATGGAAGTAGATCTTGTTGCCAAAACCTCAACCTCGGAACGAGGATTCATGCCAAATCAAAAGAGGAAACTCACCCAACAAGGCTTCCAACGTTCACAACGGCAGAAAAAAATTTCCCAAAACACTCAGAAGCATACAGTCACAACAGAGAAGCCGAACATTTACATATTCTAAGTGTAGAAATTGTGGAAAAACACCATCAGGGGCAATGTTTTCAGAACATAAGGGGTGTTTCAACTACGGCCAGACGGGTCACTATGCAAGGGCTTGCCCAAAGTTCATCAACCAAGTATCAGCTACCCAGACCACTCAGCCTACTAGTAACAAGGTTACTCAACAACAGAAAGGGACGGGTGTTTGCTGTTACACGTCAAGAGGCAGAAAATCAAGATGCAGTGGTTACAGGTACATTACCCATCCGCGAGTATTATGACTTCACTTTATTTGATTCGGGCTCTA

Protein sequence

MQNSGGTPTQRTIKGTVSWQQFNQAFYNKYFPLTVRYQKEVEFLNLRQENMLVAEYERKFDRLSHFVPRLVDTEEKKVERFIWGLREGIRAIVTAFRHKEYCSCPRVGLTHGSRSCCQNLNLGTRIHAKSKEETHPTRLPTFTTAEKNFPKHSEAYSHNREAEHLHILSVEIVEKHHQGQCFQNIRGVSTTARRVTMQGLAQSSSTKYQLPRPLSLLVTRLLNNRKGRVFAVTRQEAENQDAVVTGTLPIREYYDFTLFDSGS
Homology
BLAST of Bhi06G001165 vs. ExPASy TrEMBL
Match: A0A5A7VBY3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold616G00920 PE=4 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 4.6e-24
Identity = 93/263 (35.36%), Postives = 128/263 (48.67%), Query Frame = 0

Query: 10  QRTIKGTVS---WQQFNQAFYNKYFPLTVRYQKEVEFLNLRQENMLVAEYERKFDRLSHF 69
           +R + G VS   W+QF + FY K+F   V++ K  EFLNL Q +M V +Y+ +FD LS F
Sbjct: 387 ERMLGGDVSKITWEQFKENFYAKFFSANVKHAKLQEFLNLEQGDMTVEQYDAEFDMLSRF 446

Query: 70  VPRLVDTEEKKVERFIWGLREGIRAIVTAFRHKEYCSCPRVGLT---HGSRSCCQNLNLG 129
           +P +V  E  + E+F+ GLR  ++ IV A R   +    R+ L    H      +  +  
Sbjct: 447 LPDVVRDEAARTEKFVRGLRLDLQGIVRALRPATHADALRIALNLSLHERVDSSKAASRR 506

Query: 130 TRIHAKSKEETHPTRLPTFTTAEKN-FPKH-SEAYSHNREAEHLHILSVEIVEKHHQGQC 189
           T +  K K ET P  +P  T      F +H  E  +  R    L   +     + H+G+C
Sbjct: 507 TALGQKRKVETQPDMIPQRTLRSGGVFQRHLRELAAAGRTLRELPACTT--CGRVHEGRC 566

Query: 190 FQNIRGVSTTARRVTMQGLAQSSSTKYQLPR-PLSLLVTRLLNNRKGRVFAVTRQEAENQ 249
                GV    R        Q   T    PR P      +   +++GRVFA TRQEAE  
Sbjct: 567 LAG-SGVCFRCR--------QPGHTADPCPRKPFETTPHQPSASQQGRVFATTRQEAERA 626

Query: 250 DAVVTGTLPIREYYDFTLFDSGS 264
             VVTGTLPI  +Y F LFDSGS
Sbjct: 627 GTVVTGTLPILGHYAFVLFDSGS 638

BLAST of Bhi06G001165 vs. ExPASy TrEMBL
Match: A0A5D3BZN1 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1415G00670 PE=4 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 4.6e-24
Identity = 93/263 (35.36%), Postives = 128/263 (48.67%), Query Frame = 0

Query: 10  QRTIKGTVS---WQQFNQAFYNKYFPLTVRYQKEVEFLNLRQENMLVAEYERKFDRLSHF 69
           +R + G VS   W+QF + FY K+F   V++ K  EFLNL Q +M V +Y+ +FD LS F
Sbjct: 204 ERMLGGDVSKITWEQFKENFYAKFFSANVKHAKLQEFLNLEQGDMTVEQYDAEFDMLSRF 263

Query: 70  VPRLVDTEEKKVERFIWGLREGIRAIVTAFRHKEYCSCPRVGLT---HGSRSCCQNLNLG 129
           +P +V  E  + E+F+ GLR  ++ IV A R   +    R+ L    H      +  +  
Sbjct: 264 LPDVVRDEAARTEKFVRGLRLDLQGIVRALRPATHADALRIALNLSLHERVDSSKAASRR 323

Query: 130 TRIHAKSKEETHPTRLPTFTTAEKN-FPKH-SEAYSHNREAEHLHILSVEIVEKHHQGQC 189
           T +  K K ET P  +P  T      F +H  E  +  R    L   +     + H+G+C
Sbjct: 324 TALGQKRKVETQPDMIPQRTLRSGGVFQRHLRELAAAGRTLRELPACTT--CGRVHEGRC 383

Query: 190 FQNIRGVSTTARRVTMQGLAQSSSTKYQLPR-PLSLLVTRLLNNRKGRVFAVTRQEAENQ 249
                GV    R        Q   T    PR P      +   +++GRVFA TRQEAE  
Sbjct: 384 LAG-SGVCFRCR--------QPGHTADPCPRKPFETTPHQPSASQQGRVFATTRQEAERA 443

Query: 250 DAVVTGTLPIREYYDFTLFDSGS 264
             VVTGTLPI  +Y F LFDSGS
Sbjct: 444 GTVVTGTLPILGHYAFVLFDSGS 455

BLAST of Bhi06G001165 vs. ExPASy TrEMBL
Match: A0A5D3D772 (Gag-protease polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3037G00040 PE=4 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 7.8e-24
Identity = 87/253 (34.39%), Postives = 126/253 (49.80%), Query Frame = 0

Query: 17  VSWQQFNQAFYNKYFPLTVRYQKEVEFLNLRQENMLVAEYERKFDRLSHFVPRLVDTEEK 76
           ++W+QF ++FY K+F   V+Y K+ +FLNL Q +M+V +Y+ +FD LS F P +V  E+ 
Sbjct: 40  INWEQFKESFYTKFFSANVKYAKQQKFLNLEQGDMIVEQYDAEFDMLSRFAPNVVKDEKA 99

Query: 77  KVERFIWGLREGIRAIVTAFRHKEYCSCPRVGL---THGSRSCCQNLNLGTRIHAKSKEE 136
           + E+F+ GLR  ++ IV A R   +    R+ L    H      +    G+ +  K K E
Sbjct: 100 RTEKFVRGLRLDLQGIVRALRPTTHVDALRLVLDLSLHERADLSKVAGRGSTLRQKRKLE 159

Query: 137 THPTRLPTFTTAEKN-FPKH-SEAYSHNREAEHLHILSVEIVEKHHQGQCFQNIRGVSTT 196
           + P   P      +  F +H  E  +  R    L + S     + H G+C    R V   
Sbjct: 160 SQPDLTPQRNLRSRGVFQRHRRELAAAGRTLRELPVCS--SCGRVHGGRCLVESR-VCFK 219

Query: 197 ARRVTMQGLAQSSSTKYQLPR-PLSLLVTRLLNNRKGRVFAVTRQEAENQDAVVTGTLPI 256
            R        Q   T    PR P+     +   +++GRVFA T QEAE    VVTGTLPI
Sbjct: 220 CR--------QPRHTADACPRKPIETTPHQPPASQQGRVFATTHQEAERASTVVTGTLPI 279

Query: 257 REYYDFTLFDSGS 264
             +Y F LFDSGS
Sbjct: 280 VGHYAFVLFDSGS 281

BLAST of Bhi06G001165 vs. ExPASy TrEMBL
Match: A0A5A7VG30 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold21G003060 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.0e-23
Identity = 92/263 (34.98%), Postives = 127/263 (48.29%), Query Frame = 0

Query: 10  QRTIKGTVS---WQQFNQAFYNKYFPLTVRYQKEVEFLNLRQENMLVAEYERKFDRLSHF 69
           +R + G VS   W+QF + FY K+F   V++ K  +FLNL Q +M V +Y+ +FD LSHF
Sbjct: 161 ERMLGGDVSKITWEQFKENFYAKFFSANVKHAKLQKFLNLEQGDMTVEQYDAEFDMLSHF 220

Query: 70  VPRLVDTEEKKVERFIWGLREGIRAIVTAFRHKEYCSCPRVGLTHG---SRSCCQNLNLG 129
            P +V  E  + E+F+ GLR  ++ IV A R   +    R+ L           +    G
Sbjct: 221 APDMVRDEAARTEKFVRGLRLDLQGIVRALRPATHADALRIALDLSLPERADSSKAAGRG 280

Query: 130 TRIHAKSKEETHPTRLPTFTTAEKN-FPKH-SEAYSHNREAEHLHILSVEIVEKHHQGQC 189
           + +  K K ET P  +P  T      F +H  E  +  R    L   +    E+ H G+C
Sbjct: 281 SALGQKGKVETQPDVVPQQTLRSGGVFQRHRRELAAAGRTLRELPTCTT--CERVHGGRC 340

Query: 190 FQNIRGVSTTARRVTMQGLAQSSSTKYQLPR-PLSLLVTRLLNNRKGRVFAVTRQEAENQ 249
                GV    R        Q   T    PR P      +   +++GRVFA TRQ+AE  
Sbjct: 341 LAG-SGVCFRCR--------QPGHTADVCPRKPFETTPPQPSASQQGRVFATTRQDAERA 400

Query: 250 DAVVTGTLPIREYYDFTLFDSGS 264
             VVTGTLPI  +Y F LFDSGS
Sbjct: 401 GTVVTGTLPILGHYAFVLFDSGS 412

BLAST of Bhi06G001165 vs. ExPASy TrEMBL
Match: A0A5A7VMR4 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold550G00890 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 1.7e-23
Identity = 88/253 (34.78%), Postives = 123/253 (48.62%), Query Frame = 0

Query: 17  VSWQQFNQAFYNKYFPLTVRYQKEVEFLNLRQENMLVAEYERKFDRLSHFVPRLVDTEEK 76
           ++W+QF + FY K+F   V++ K  EFLNL Q +M V +Y+ +FD LS F P +V  E  
Sbjct: 9   ITWEQFKENFYAKFFSANVKHAKLQEFLNLEQGDMTVEQYDVEFDMLSRFAPDVVRDEAA 68

Query: 77  KVERFIWGLREGIRAIVTAFRHKEYCSCPRVGL---THGSRSCCQNLNLGTRIHAKSKEE 136
           + E+F+ GLR  ++ IV A R   +    R+ L    H      +    G  +  K K E
Sbjct: 69  RTEKFVRGLRLDLQGIVRALRPATHADALRIALDLSLHERADSSKAAGRGLALGQKRKVE 128

Query: 137 THPTRLPTFTTAEKN-FPKH-SEAYSHNREAEHLHILSVEIVEKHHQGQCFQNIRGVSTT 196
           T P  +P  T   +  F +H  E  +  R    L  L+     + H+G+C     GV   
Sbjct: 129 TQPDVVPQRTLRSRGVFQRHRRELAAAGRTLREL--LACTTCGRVHEGRCLAG-SGVCFK 188

Query: 197 ARRVTMQGLAQSSSTKYQLPR-PLSLLVTRLLNNRKGRVFAVTRQEAENQDAVVTGTLPI 256
            R        Q   T    PR P      +   +++GRVFA TRQEAE    VV GTLPI
Sbjct: 189 CR--------QPGHTADVCPRKPFETTPPQPSASQQGRVFATTRQEAERAGTVVIGTLPI 248

Query: 257 REYYDFTLFDSGS 264
             +Y F +FDSGS
Sbjct: 249 LGHYAFVVFDSGS 250

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7VBY34.6e-2435.36Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold61... [more]
A0A5D3BZN14.6e-2435.36Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold14... [more]
A0A5D3D7727.8e-2434.39Gag-protease polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A5A7VG301.0e-2334.98Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold21... [more]
A0A5A7VMR41.7e-2334.78Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold55... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005162Retrotransposon gag domainPFAMPF03732Retrotrans_gagcoord: 10..87
e-value: 2.4E-15
score: 56.6
NoneNo IPR availablePFAMPF08284RVP_2coord: 226..262
e-value: 8.1E-5
score: 22.4
NoneNo IPR availablePANTHERPTHR34482:SF4POLYMERASES SUPERFAMILY PROTEIN, PUTATIVE ISOFORM 1-RELATEDcoord: 14..187
NoneNo IPR availablePANTHERPTHR34482DNA DAMAGE-INDUCIBLE PROTEIN 1-LIKEcoord: 14..187

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi06M001165Bhi06M001165mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003824 catalytic activity