Bhi06G001140 (gene) Wax gourd (B227) v1

Overview
NameBhi06G001140
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionNADPH--cytochrome P450 reductase
Locationchr6: 38198340 .. 38198501 (-)
RNA-Seq ExpressionBhi06G001140
SyntenyBhi06G001140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGGATCAATTGCTTCGAAATGAGGATGATGTAACAATAGTGACCACACCTTACACGGCTGCCATATCAGAATATCGAGTGGTATTCTATGATCCTGTAGATGTGACTGATGACAACAAGAACTGGATGAATGCAAATGGTCATGTTGTACATGATGCTTAA

mRNA sequence

TTGGATCAATTGCTTCGAAATGAGGATGATGTAACAATAGTGACCACACCTTACACGGCTGCCATATCAGAATATCGAGTGGTATTCTATGATCCTGTAGATGTGACTGATGACAACAAGAACTGGATGAATGCAAATGGTCATGTTGTACATGATGCTTAA

Coding sequence (CDS)

TTGGATCAATTGCTTCGAAATGAGGATGATGTAACAATAGTGACCACACCTTACACGGCTGCCATATCAGAATATCGAGTGGTATTCTATGATCCTGTAGATGTGACTGATGACAACAAGAACTGGATGAATGCAAATGGTCATGTTGTACATGATGCTTAA

Protein sequence

LDQLLRNEDDVTIVTTPYTAAISEYRVVFYDPVDVTDDNKNWMNANGHVVHDA
Homology
[Close]
BLAST of Bhi06G001140 vs. TAIR 10
Match: AT4G30210.1 (P450 reductase 2 )

HSP 1 Score: 51.2 bits (121), Expect = 2.9e-07
Identity = 27/53 (50.94%), Postives = 33/53 (62.26%), Query Frame = 0 

Query: 1   LDQLLRNEDDVTIVTTPYTAAISEYRVVFYDPVDVTDDNKNWMNANGHVVHDA 54
           LD +LR E D T V TPYTAA+ EYRV  +D  D   ++ N  N NG+ V DA
Sbjct: 258 LDTILREEGD-TAVATPYTAAVLEYRVSIHDSEDAKFNDINMANGNGYTVFDA 309

[Close]
BLAST of Bhi06G001140 vs. TAIR 10
Match: AT4G30210.2 (P450 reductase 2 )

HSP 1 Score: 51.2 bits (121), Expect = 2.9e-07
Identity = 27/53 (50.94%), Postives = 33/53 (62.26%), Query Frame = 0 

Query: 1   LDQLLRNEDDVTIVTTPYTAAISEYRVVFYDPVDVTDDNKNWMNANGHVVHDA 54
           LD +LR E D T V TPYTAA+ EYRV  +D  D   ++ N  N NG+ V DA
Sbjct: 258 LDTILREEGD-TAVATPYTAAVLEYRVSIHDSEDAKFNDINMANGNGYTVFDA 309

[Close]
BLAST of Bhi06G001140 vs. TAIR 10
Match: AT4G24520.1 (P450 reductase 1 )

HSP 1 Score: 46.6 bits (109), Expect = 7.2e-06
Identity = 26/52 (50.00%), Postives = 33/52 (63.46%), Query Frame = 0 

Query: 1   LDQLLRNEDDVTIVTTPYTAAISEYRVVFYDPVDVTDDNKNWMNANGHVVHD 53
           LD+LL++EDD + V TPYTA I EYRVV +DP   T  +     ANG+   D
Sbjct: 238 LDKLLKDEDDKS-VATPYTAVIPEYRVVTHDPRFTTQKSMESNVANGNTTID 288

[Close]
BLAST of Bhi06G001140 vs. TAIR 10
Match: AT4G24520.2 (P450 reductase 1 )

HSP 1 Score: 46.6 bits (109), Expect = 7.2e-06
Identity = 26/52 (50.00%), Postives = 33/52 (63.46%), Query Frame = 0 

Query: 1   LDQLLRNEDDVTIVTTPYTAAISEYRVVFYDPVDVTDDNKNWMNANGHVVHD 53
           LD+LL++EDD + V TPYTA I EYRVV +DP   T  +     ANG+   D
Sbjct: 234 LDKLLKDEDDKS-VATPYTAVIPEYRVVTHDPRFTTQKSMESNVANGNTTID 284

[Close]
BLAST of Bhi06G001140 vs. ExPASy Swiss-Prot
Match: Q7X7K8 (NADPH--cytochrome P450 reductase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CPR1 PE=1 SV=2)

HSP 1 Score: 64.7 bits (156), Expect = 3.6e-10
Identity = 32/52 (61.54%), Postives = 38/52 (73.08%), Query Frame = 0 

Query: 1   LDQLLRNEDDVTIVTTPYTAAISEYRVVFYDPVDVTDDNKNWMNANGHVVHD 53
           LDQLLR+EDD T  +TPYTAAI EYR+VF D  DV+  +K+W  ANG  V D
Sbjct: 238 LDQLLRDEDDTTGASTPYTAAIPEYRIVFIDKSDVSFQDKSWSLANGSGVID 289

[Close]
BLAST of Bhi06G001140 vs. ExPASy Swiss-Prot
Match: A0A2U1LIM9 (NADPH--cytochrome P450 reductase 1 OS=Artemisia annua OX=35608 GN=CPR1 PE=1 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 5.2e-09
Identity = 32/53 (60.38%), Postives = 39/53 (73.58%), Query Frame = 0 

Query: 1   LDQLLRNEDDVTIVTTPYTAAISEYRVVFYDPVDVTDDNKNWMNANGHVVHDA 54
           LDQLLR+EDD T V TPYTAA++EYRVVF+D  +  D ++     NGH VHDA
Sbjct: 253 LDQLLRDEDD-TSVATPYTAAVAEYRVVFHDKPETYDQDQ---LTNGHAVHDA 301

[Close]
BLAST of Bhi06G001140 vs. ExPASy Swiss-Prot
Match: Q653S9 (NADPH--cytochrome P450 reductase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CPR3 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 5.8e-08
Identity = 28/52 (53.85%), Postives = 35/52 (67.31%), Query Frame = 0 

Query: 1   LDQLLRNEDDVTIVTTPYTAAISEYRVVFYDPVDVTDDNKNWMNANGHVVHD 53
           LD+LLR EDD +   TPYTAAI EYRVV   P +    NK++  +NGH V+D
Sbjct: 258 LDKLLRVEDDKSAAPTPYTAAIPEYRVVLVKPEEAMHINKSFSLSNGHAVYD 309

[Close]
BLAST of Bhi06G001140 vs. ExPASy Swiss-Prot
Match: P37116 (NADPH--cytochrome P450 reductase OS=Vigna radiata var. radiata OX=3916 PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 1.3e-07
Identity = 28/52 (53.85%), Postives = 35/52 (67.31%), Query Frame = 0 

Query: 1   LDQLLRNEDDVTIVTTPYTAAISEYRVVFYDPVDVTDDNKNWMNANGHVVHD 53
           LDQLLR+EDD   V+TPYTAAI EYRVV +DP   +  + +   ANG+   D
Sbjct: 236 LDQLLRDEDDANTVSTPYTAAILEYRVVIHDPTAASTYDNHSTVANGNTEFD 287

[Close]
BLAST of Bhi06G001140 vs. ExPASy Swiss-Prot
Match: A0A2U1KZS6 (NADPH--cytochrome P450 reductase 2 OS=Artemisia annua OX=35608 GN=CPR2 PE=3 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 2.2e-07
Identity = 29/53 (54.72%), Postives = 40/53 (75.47%), Query Frame = 0 

Query: 1   LDQLLRNEDDVTIVTTPYTAAISEYRVVFYDPVDVTDDNKNWMNANGHVVHDA 54
           LDQLLR+EDD++ V TPYTAA+ EYRVV++D  D + ++ +  + NGH V DA
Sbjct: 257 LDQLLRDEDDMS-VATPYTAAVLEYRVVYHDKPDSSAEDHS--HTNGHAVPDA 306

[Close]
BLAST of Bhi06G001140 vs. ExPASy TrEMBL
Match: A0A5A7USX7 (NADPH--cytochrome P450 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G006000 PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 9.8e-19
Identity = 46/53 (86.79%), Postives = 48/53 (90.57%), Query Frame = 0 

Query: 1   LDQLLRNEDDVTIVTTPYTAAISEYRVVFYDPVDVTDDNKNWMNANGHVVHDA 54
           LDQLLR+EDD   VTTPYTAAISEYRVVF+DP DVTDDNKNWMNANGH VHDA
Sbjct: 253 LDQLLRDEDDAATVTTPYTAAISEYRVVFHDPADVTDDNKNWMNANGHAVHDA 305

[Close]
BLAST of Bhi06G001140 vs. ExPASy TrEMBL
Match: A0A5D3CN13 (NADPH--cytochrome P450 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00410 PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 9.8e-19
Identity = 46/53 (86.79%), Postives = 48/53 (90.57%), Query Frame = 0 

Query: 1   LDQLLRNEDDVTIVTTPYTAAISEYRVVFYDPVDVTDDNKNWMNANGHVVHDA 54
           LDQLLR+EDD   VTTPYTAAISEYRVVF+DP DVTDDNKNWMNANGH VHDA
Sbjct: 253 LDQLLRDEDDAATVTTPYTAAISEYRVVFHDPADVTDDNKNWMNANGHAVHDA 305

[Close]
BLAST of Bhi06G001140 vs. ExPASy TrEMBL
Match: A0A1S3BXG0 (NADPH--cytochrome P450 reductase OS=Cucumis melo OX=3656 GN=LOC103494263 PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 9.8e-19
Identity = 46/53 (86.79%), Postives = 48/53 (90.57%), Query Frame = 0 

Query: 1   LDQLLRNEDDVTIVTTPYTAAISEYRVVFYDPVDVTDDNKNWMNANGHVVHDA 54
           LDQLLR+EDD   VTTPYTAAISEYRVVF+DP DVTDDNKNWMNANGH VHDA
Sbjct: 253 LDQLLRDEDDAATVTTPYTAAISEYRVVFHDPADVTDDNKNWMNANGHAVHDA 305

[Close]
BLAST of Bhi06G001140 vs. ExPASy TrEMBL
Match: A0A0A0LXA1 (NADPH--cytochrome P450 reductase OS=Cucumis sativus OX=3659 GN=Csa1G423150 PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 8.3e-18
Identity = 45/53 (84.91%), Postives = 47/53 (88.68%), Query Frame = 0 

Query: 1   LDQLLRNEDDVTIVTTPYTAAISEYRVVFYDPVDVTDDNKNWMNANGHVVHDA 54
           LDQLLR+EDD   VTTPYTAAISEYRVVF+DP DVTDD KNWMNANGH VHDA
Sbjct: 253 LDQLLRDEDDAATVTTPYTAAISEYRVVFHDPSDVTDDKKNWMNANGHAVHDA 305

[Close]
BLAST of Bhi06G001140 vs. ExPASy TrEMBL
Match: A0A6J1JGV7 (NADPH--cytochrome P450 reductase OS=Cucurbita maxima OX=3661 GN=LOC111484987 PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.9e-17
Identity = 44/53 (83.02%), Postives = 47/53 (88.68%), Query Frame = 0 

Query: 1   LDQLLRNEDDVTIVTTPYTAAISEYRVVFYDPVDVTDDNKNWMNANGHVVHDA 54
           LDQLLR+EDD T VTTPYTAAISEYRVVF+DP  V D+NKNWMNANGH VHDA
Sbjct: 253 LDQLLRDEDDATTVTTPYTAAISEYRVVFHDPAGVADENKNWMNANGHAVHDA 305

The following BLAST results are available for this feature:
BLAST of Bhi06G001140 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G30210.12.9e-0750.94P450 reductase 2 [more]
AT4G30210.22.9e-0750.94P450 reductase 2 [more]
AT4G24520.17.2e-0650.00P450 reductase 1 [more]
AT4G24520.27.2e-0650.00P450 reductase 1 [more]
BLAST of Bhi06G001140 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q7X7K83.6e-1061.54NADPH--cytochrome P450 reductase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
A0A2U1LIM95.2e-0960.38NADPH--cytochrome P450 reductase 1 OS=Artemisia annua OX=35608 GN=CPR1 PE=1 SV=2[more]
Q653S95.8e-0853.85NADPH--cytochrome P450 reductase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
P371161.3e-0753.85NADPH--cytochrome P450 reductase OS=Vigna radiata var. radiata OX=3916 PE=1 SV=1[more]
A0A2U1KZS62.2e-0754.72NADPH--cytochrome P450 reductase 2 OS=Artemisia annua OX=35608 GN=CPR2 PE=3 SV=1[more]
BLAST of Bhi06G001140 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7USX79.8e-1986.79NADPH--cytochrome P450 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5D3CN139.8e-1986.79NADPH--cytochrome P450 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BXG09.8e-1986.79NADPH--cytochrome P450 reductase OS=Cucumis melo OX=3656 GN=LOC103494263 PE=3 SV... [more]
A0A0A0LXA18.3e-1884.91NADPH--cytochrome P450 reductase OS=Cucumis sativus OX=3659 GN=Csa1G423150 PE=2 ... [more]
A0A6J1JGV71.9e-1783.02NADPH--cytochrome P450 reductase OS=Cucurbita maxima OX=3661 GN=LOC111484987 PE=... [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi06M001140Bhi06M001140mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005829 cytosol
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0010181 FMN binding
molecular_function GO:0050661 NADP binding
molecular_function GO:0003958 NADPH-hemoprotein reductase activity