Bhi06G000925 (gene) Wax gourd (B227) v1

Overview


Name	Bhi06G000925
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	TPX2 domain-containing protein
Location	chr6: 29132642 .. 29133239 (-)
RNA-Seq Expression	Bhi06G000925
Synteny	Bhi06G000925

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAGAGGAAGAGAGACAAAGAATACCAATTGCTCAAGGTCTTCCATGGACAACTGATGAACCAGAGGTATGTGTTGAACTTTCAGCTCTTGAATGCACCATTAGTGCTTAAAAGAAAAATGTCCATATCCATTTGTAGTGCCTTGTTAAACCCCCTGTGAAGGAAATTACAATACCAGTGGATCTGGTTCTCCACAGTGATGTAAGGGCAATAGAACGCGCTGAGTTCGATCATCAGGTGGTTTTATGATCTCAGGAAAGAACATCTGTTAATCCCTTGTGAAAAGTAAAGTAATATGATATATGGCCTGCAACCCAAATTCATAAATCCATGTTTTTTTTTTTGTTAATGAGAAGGTAGCAGAGAAGTTGTCTGTGATAGAACAATACAAGATGGAAAGAGAGAAACAACATAAGGTATAGAAAAAAAAATGAAATGGCTATCAATTCAATTCATATTATGTGTTGATTTGTTTGGTTTTTGTGGTAACTAACAGATGGTTGAGGAGGAAGAACTAAAGAGGTTGAGAAAGGAATTGGTTCCAAAGGCACAACTAATGCCTTATTTTGATAGGCTTTTCATTACTAGAAGGTAA

mRNA sequence

ATGATAGAGGAAGAGAGACAAAGAATACCAATTGCTCAAGGTCTTCCATGGACAACTGATGAACCAGAGTGCCTTGTTAAACCCCCTGTGAAGGAAATTACAATACCAGTGGATCTGGTTCTCCACAGTGATGTAAGGGCAATAGAACGCGCTGAGTTCGATCATCAGGTAGCAGAGAAGTTGTCTGTGATAGAACAATACAAGATGGAAAGAGAGAAACAACATAAGATGGTTGAGGAGGAAGAACTAAAGAGGTTGAGAAAGGAATTGGTTCCAAAGGCACAACTAATGCCTTATTTTGATAGGCTTTTCATTACTAGAAGGTAA

Coding sequence (CDS)

ATGATAGAGGAAGAGAGACAAAGAATACCAATTGCTCAAGGTCTTCCATGGACAACTGATGAACCAGAGTGCCTTGTTAAACCCCCTGTGAAGGAAATTACAATACCAGTGGATCTGGTTCTCCACAGTGATGTAAGGGCAATAGAACGCGCTGAGTTCGATCATCAGGTAGCAGAGAAGTTGTCTGTGATAGAACAATACAAGATGGAAAGAGAGAAACAACATAAGATGGTTGAGGAGGAAGAACTAAAGAGGTTGAGAAAGGAATTGGTTCCAAAGGCACAACTAATGCCTTATTTTGATAGGCTTTTCATTACTAGAAGGTAA

Protein sequence

MIEEERQRIPIAQGLPWTTDEPECLVKPPVKEITIPVDLVLHSDVRAIERAEFDHQVAEKLSVIEQYKMEREKQHKMVEEEELKRLRKELVPKAQLMPYFDRLFITRR

Homology

[Close]

BLAST of Bhi06G000925 vs. TAIR 10
Match: AT5G15510.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 169.1 bits (427), Expect = 2.0e-42
Identity = 86/107 (80.37%), Postives = 96/107 (89.72%), Query Frame = 0


Query: 2   IEEERQRIPIAQGLPWTTDEPECLVKPPVKEITIPVDLVLHSDVRAIERAEFDHQVAEKL 61
           +EEE+ RIPIAQGLPWTTDEPECLVKP  K+IT PVDL LHSDVRA+ERAEFD+QVAEK+
Sbjct: 347 MEEEKMRIPIAQGLPWTTDEPECLVKPHWKDITRPVDLTLHSDVRAVERAEFDYQVAEKM 406

Query: 62  SVIEQYKMEREKQHKMVEEEELKRLRKELVPKAQLMPYFDRLFITRR 109
           S IEQYKMERE+Q K+ EEEE++RLRKELVPKAQ MPYFDR FI RR
Sbjct: 407 SFIEQYKMERERQQKLAEEEEIRRLRKELVPKAQPMPYFDRPFIPRR 453

[Close]

BLAST of Bhi06G000925 vs. TAIR 10
Match: AT5G15510.2 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 169.1 bits (427), Expect = 2.0e-42
Identity = 86/107 (80.37%), Postives = 96/107 (89.72%), Query Frame = 0


Query: 2   IEEERQRIPIAQGLPWTTDEPECLVKPPVKEITIPVDLVLHSDVRAIERAEFDHQVAEKL 61
           +EEE+ RIPIAQGLPWTTDEPECLVKP  K+IT PVDL LHSDVRA+ERAEFD+QVAEK+
Sbjct: 347 MEEEKMRIPIAQGLPWTTDEPECLVKPHWKDITRPVDLTLHSDVRAVERAEFDYQVAEKM 406

Query: 62  SVIEQYKMEREKQHKMVEEEELKRLRKELVPKAQLMPYFDRLFITRR 109
           S IEQYKMERE+Q K+ EEEE++RLRKELVPKAQ MPYFDR FI RR
Sbjct: 407 SFIEQYKMERERQQKLAEEEEIRRLRKELVPKAQPMPYFDRPFIPRR 453

[Close]

BLAST of Bhi06G000925 vs. TAIR 10
Match: AT3G01015.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 159.8 bits (403), Expect = 1.2e-39
Identity = 79/107 (73.83%), Postives = 96/107 (89.72%), Query Frame = 0


Query: 2   IEEERQRIPIAQGLPWTTDEPECLVKPPVKEITIPVDLVLHSDVRAIERAEFDHQVAEKL 61
           +E+ ++RIPIAQGLPWTTDEPE LVKP VK+ITIPVDL LHSD+RA+ERAEFD+QV EK+
Sbjct: 337 LEKAKKRIPIAQGLPWTTDEPENLVKPHVKDITIPVDLKLHSDIRAVERAEFDYQVTEKI 396

Query: 62  SVIEQYKMEREKQHKMVEEEELKRLRKELVPKAQLMPYFDRLFITRR 109
           +++EQYK ERE+Q K+ EEEE++RLRKELVPKAQ MPYFDR FI +R
Sbjct: 397 NLVEQYKTERERQQKLAEEEEIRRLRKELVPKAQPMPYFDRPFIPKR 443

[Close]

BLAST of Bhi06G000925 vs. TAIR 10
Match: AT5G37478.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 75.5 bits (184), Expect = 3.0e-14
Identity = 40/84 (47.62%), Postives = 58/84 (69.05%), Query Frame = 0


Query: 25  LVKPPVKEITIPVDLVLHSDVRAIERAEFDHQVAEKLSVIEQYKMEREKQHKMVEEEELK 84
           + K P KE   P++  LHS  RA++RA F++ VA    + +  K + E+  KM+EEEE++
Sbjct: 56  ITKAPAKENKKPLEFKLHSGERAVKRAMFNYSVATNYYIQKLQKKQEERLQKMIEEEEIR 115

Query: 85  RLRKELVPKAQLMPYFDRLFITRR 109
            LRKE+VPKAQLMP+FDR F+ +R
Sbjct: 116 MLRKEMVPKAQLMPFFDRPFLPQR 139

[Close]

BLAST of Bhi06G000925 vs. TAIR 10
Match: AT1G03780.2 (targeting protein for XKLP2 )

HSP 1 Score: 53.5 bits (127), Expect = 1.2e-07
Identity = 33/94 (35.11%), Postives = 56/94 (59.57%), Query Frame = 0


Query: 27  KPPVKEITIPV------------DLVLHSDVRAIERAEFDHQVAEKLSVIEQYKMEREKQ 86
           +P +KE  IPV            +  LH + RA+ERA+FDH++ EK +  ++Y+ E E  
Sbjct: 623 QPVIKEDPIPVPEKVRMPLTEIQEFNLHVEHRAVERADFDHKIKEKENQYKRYREESEAA 682

Query: 87  HKMVEEEELKRLRKELVPKAQLMPYFDRLFITRR 109
             + EE  LK++RK +VP A+ +P F++ F+ ++
Sbjct: 683 KMVEEERALKQMRKTMVPHARPVPNFNKPFLPQK 716

[Close]

BLAST of Bhi06G000925 vs. ExPASy Swiss-Prot
Match: F4I2H7 (Protein TPX2 OS=Arabidopsis thaliana OX=3702 GN=TPX2 PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.7e-06
Identity = 33/94 (35.11%), Postives = 56/94 (59.57%), Query Frame = 0


Query: 27  KPPVKEITIPV------------DLVLHSDVRAIERAEFDHQVAEKLSVIEQYKMEREKQ 86
           +P +KE  IPV            +  LH + RA+ERA+FDH++ EK +  ++Y+ E E  
Sbjct: 623 QPVIKEDPIPVPEKVRMPLTEIQEFNLHVEHRAVERADFDHKIKEKENQYKRYREESEAA 682

Query: 87  HKMVEEEELKRLRKELVPKAQLMPYFDRLFITRR 109
             + EE  LK++RK +VP A+ +P F++ F+ ++
Sbjct: 683 KMVEEERALKQMRKTMVPHARPVPNFNKPFLPQK 716

[Close]

BLAST of Bhi06G000925 vs. ExPASy TrEMBL
Match: A0A1S3CAJ5 (uncharacterized protein LOC103498525 OS=Cucumis melo OX=3656 GN=LOC103498525 PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.1e-45
Identity = 100/108 (92.59%), Postives = 102/108 (94.44%), Query Frame = 0


Query: 1   MIEEERQRIPIAQGLPWTTDEPECLVKPPVKEITIPVDLVLHSDVRAIERAEFDHQVAEK 60
           MIEEERQRIPIAQGLPWTTDEPECLVKPPVKEIT PVDLVLHSDVRA+ERAEFDHQVAEK
Sbjct: 367 MIEEERQRIPIAQGLPWTTDEPECLVKPPVKEITKPVDLVLHSDVRAVERAEFDHQVAEK 426

Query: 61  LSVIEQYKMEREKQHKMVEEEELKRLRKELVPKAQLMPYFDRLFITRR 109
           LSVIEQYKMEREKQ KM EEEEL+RLRKELVPKAQ MPYFDR FI RR
Sbjct: 427 LSVIEQYKMEREKQQKMAEEEELRRLRKELVPKAQPMPYFDRPFIPRR 474

[Close]

BLAST of Bhi06G000925 vs. ExPASy TrEMBL
Match: A0A0A0KVS9 (TPX2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G638410 PE=3 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.5e-45
Identity = 99/108 (91.67%), Postives = 102/108 (94.44%), Query Frame = 0


Query: 1   MIEEERQRIPIAQGLPWTTDEPECLVKPPVKEITIPVDLVLHSDVRAIERAEFDHQVAEK 60
           MIEEE+QRIPIAQGLPWTTDEPEC+VKPPVKEIT PVDLVLHSDVRA+ERAEFDHQVAEK
Sbjct: 367 MIEEEKQRIPIAQGLPWTTDEPECVVKPPVKEITRPVDLVLHSDVRAVERAEFDHQVAEK 426

Query: 61  LSVIEQYKMEREKQHKMVEEEELKRLRKELVPKAQLMPYFDRLFITRR 109
           LSVIEQYKMEREKQ KM EEEELKRLRKELVPKAQ MPYFDR FI RR
Sbjct: 427 LSVIEQYKMEREKQEKMAEEEELKRLRKELVPKAQPMPYFDRPFIPRR 474

[Close]

BLAST of Bhi06G000925 vs. ExPASy TrEMBL
Match: A0A6J1FFJ6 (protein WVD2-like 4 OS=Cucurbita moschata OX=3662 GN=LOC111445027 PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.2e-42
Identity = 94/108 (87.04%), Postives = 101/108 (93.52%), Query Frame = 0


Query: 1   MIEEERQRIPIAQGLPWTTDEPECLVKPPVKEITIPVDLVLHSDVRAIERAEFDHQVAEK 60
           M+E+ERQRIPIAQGLPWTTDEPECLVKPPVKE T PVDLVLHSDVRA+ERAEFD+QVAEK
Sbjct: 418 MMEQERQRIPIAQGLPWTTDEPECLVKPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEK 477

Query: 61  LSVIEQYKMEREKQHKMVEEEELKRLRKELVPKAQLMPYFDRLFITRR 109
           LS+IEQYKM+REKQ KM EEEEL+RLRKELVPKAQ MPYFDR FI RR
Sbjct: 478 LSLIEQYKMDREKQQKMAEEEELRRLRKELVPKAQPMPYFDRPFIPRR 525

[Close]

BLAST of Bhi06G000925 vs. ExPASy TrEMBL
Match: A0A6J1H166 (uncharacterized protein LOC111459130 OS=Cucurbita moschata OX=3662 GN=LOC111459130 PE=3 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 2.0e-42
Identity = 92/108 (85.19%), Postives = 101/108 (93.52%), Query Frame = 0


Query: 1   MIEEERQRIPIAQGLPWTTDEPECLVKPPVKEITIPVDLVLHSDVRAIERAEFDHQVAEK 60
           MIEEERQRIPIAQGLPWTTDEPECLVKPPVKE T PVDLVLHSDVRA+ERA+FDHQVA+K
Sbjct: 373 MIEEERQRIPIAQGLPWTTDEPECLVKPPVKENTRPVDLVLHSDVRAVERADFDHQVAKK 432

Query: 61  LSVIEQYKMEREKQHKMVEEEELKRLRKELVPKAQLMPYFDRLFITRR 109
           L+++EQYKMERE+Q KM EEEE++RLRKELVPKAQ MPYFDR FI RR
Sbjct: 433 LTLLEQYKMERERQQKMAEEEEVRRLRKELVPKAQPMPYFDRPFIPRR 480

[Close]

BLAST of Bhi06G000925 vs. ExPASy TrEMBL
Match: A0A6J1K2M1 (uncharacterized protein LOC111489526 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489526 PE=3 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 3.4e-42
Identity = 94/108 (87.04%), Postives = 100/108 (92.59%), Query Frame = 0


Query: 1   MIEEERQRIPIAQGLPWTTDEPECLVKPPVKEITIPVDLVLHSDVRAIERAEFDHQVAEK 60
           MIE+ERQRIPIAQGLPWTTDEPECLVKPPVK  T PVDLVLHSDVRA+ERAEFD+QVAEK
Sbjct: 420 MIEQERQRIPIAQGLPWTTDEPECLVKPPVKGNTRPVDLVLHSDVRAVERAEFDNQVAEK 479

Query: 61  LSVIEQYKMEREKQHKMVEEEELKRLRKELVPKAQLMPYFDRLFITRR 109
           LS+IEQYKM+REKQ KM EEEEL+RLRKELVPKAQ MPYFDR FI RR
Sbjct: 480 LSLIEQYKMDREKQQKMAEEEELRRLRKELVPKAQPMPYFDRPFIPRR 527

The following BLAST results are available for this feature:

BLAST of Bhi06G000925 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G15510.1	2.0e-42	80.37	TPX2 (targeting protein for Xklp2) protein family	[more]
AT5G15510.2	2.0e-42	80.37	TPX2 (targeting protein for Xklp2) protein family	[more]
AT3G01015.1	1.2e-39	73.83	TPX2 (targeting protein for Xklp2) protein family	[more]
AT5G37478.1	3.0e-14	47.62	TPX2 (targeting protein for Xklp2) protein family	[more]
AT1G03780.2	1.2e-07	35.11	targeting protein for XKLP2	[more]

BLAST of Bhi06G000925 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I2H7	1.7e-06	35.11	Protein TPX2 OS=Arabidopsis thaliana OX=3702 GN=TPX2 PE=1 SV=1	[more]

BLAST of Bhi06G000925 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CAJ5	1.1e-45	92.59	uncharacterized protein LOC103498525 OS=Cucumis melo OX=3656 GN=LOC103498525 PE=...	[more]
A0A0A0KVS9	2.5e-45	91.67	TPX2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G638410 PE=3 S...	[more]
A0A6J1FFJ6	1.2e-42	87.04	protein WVD2-like 4 OS=Cucurbita moschata OX=3662 GN=LOC111445027 PE=3 SV=1	[more]
A0A6J1H166	2.0e-42	85.19	uncharacterized protein LOC111459130 OS=Cucurbita moschata OX=3662 GN=LOC1114591...	[more]
A0A6J1K2M1	3.4e-42	87.04	uncharacterized protein LOC111489526 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 64..89
None	No IPR available	PANTHER	PTHR14326:SF45	TPX2 (TARGETING PROTEIN FOR XKLP2) PROTEIN FAMILY-RELATED	coord: 1..108
IPR027329	TPX2, C-terminal	PFAM	PF06886	TPX2	coord: 40..107 e-value: 1.3E-21 score: 76.5
IPR009675	TPX2	PANTHER	PTHR14326	TARGETING PROTEIN FOR XKLP2	coord: 1..108

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi06M000925	Bhi06M000925	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032147	activation of protein kinase activity
biological_process	GO:0090307	mitotic spindle assembly
biological_process	GO:0060236	regulation of mitotic spindle organization
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0072686	mitotic spindle
cellular_component	GO:0005880	nuclear microtubule
cellular_component	GO:0005874	microtubule
cellular_component	GO:0005819	spindle
molecular_function	GO:0008017	microtubule binding
molecular_function	GO:0030295	protein kinase activator activity