Bhi06G000803 (gene) Wax gourd (B227) v1

Overview
NameBhi06G000803
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionNB-ARC domain-containing disease resistance protein
Locationchr6: 24856784 .. 24857451 (+)
RNA-Seq ExpressionBhi06G000803
SyntenyBhi06G000803
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCTGTGAAGTAACAAGAAATGTTGAAAACATAGAATCTGGTGTCCAAAATTGGTTGACCAAGACTGACTCCATCATTGAAAAATCTGAAACATTCCTAAACAACCTTTCTCAACAAGGTTGATTGTGCTTGAATTTGGTCCGAAGACACCAATTAAGTAGGAAAGTTGTAAAGTTGGTTGATGAGGTTGTTGAGATGAAAATGAGGGAAATTTTGATAGAGTTTCCTATCGTGTAGCTCTCTCAGTGGTTGAGAGTTCAAAGGCAAAGACTTCAGATTTTATTAACTTTGAATCAAGAAAGCCAAATATAGACAAAATCATTGGTTCACTTATGGATGACAATGTCCACGAAATTGGAGTGTATGGGATGAGAGGTGTTGGCAAAACAATGTTAGTGCAATAGATCTCGAGAATAGTAATGGAGGAAAAGTTATTTGATGAAGTGGTCATATCGACCATAAGTCCAACCCTGGACTTAAAAAAAATTCAAGGGCAACTTGATGATATGCTTGGGTTGAAATTTGACCAAGAAATAGAGGAAAGAATTACCCTTATGCTACATAAAAGGTTGAAGATGGAACAAAGTATCCTCGTTGTACTTGATGATGAATGGAAGCAAAATGATTTGGAAATGATAGGAATTCCAAGCATTGAAGATCGCATATGA

mRNA sequence

GTCTGTGAAGTAACAAGAAATGTTGAAAACATAGAATCTGGTGTCCAAAATTGGTTGACCAAGACTGACTCCATCATTGAAAAATCTGAAACATTCCTAAACAACCTTTCTCAACAAGTTGTAAAGTTGGTTGATGAGGTTGTTGAGATGAAAATGAGGGAAATTTTGATAGAGTTTCCTATCAGTTCAAAGGCAAAGACTTCAGATTTTATTAACTTTGAATCAAGAAAGCCAAATATAGACAAAATCATTGGTTCACTTATGGATGACAATGTCCACGAAATTGGAGTGTATGGGATGAGAGGTATCTCGAGAATAGTAATGGAGGAAAAGTTATTTGATGAAGTGGTCATATCGACCATAAGGCAACTTGATGATATGCTTGGGTTGAAATTTGACCAAGAAATAGAGGAAAGAATTACCCTTATGCTACATAAAAGGTTGAAGATGGAACAAAGTATCCTCGTTGTACTTGATGATGAATGGAAGCAAAATGATTTGGAAATGATAGGAATTCCAAGCATTGAAGATCGCATATGA

Coding sequence (CDS)

GTCTGTGAAGTAACAAGAAATGTTGAAAACATAGAATCTGGTGTCCAAAATTGGTTGACCAAGACTGACTCCATCATTGAAAAATCTGAAACATTCCTAAACAACCTTTCTCAACAAGTTGTAAAGTTGGTTGATGAGGTTGTTGAGATGAAAATGAGGGAAATTTTGATAGAGTTTCCTATCAGTTCAAAGGCAAAGACTTCAGATTTTATTAACTTTGAATCAAGAAAGCCAAATATAGACAAAATCATTGGTTCACTTATGGATGACAATGTCCACGAAATTGGAGTGTATGGGATGAGAGGTATCTCGAGAATAGTAATGGAGGAAAAGTTATTTGATGAAGTGGTCATATCGACCATAAGGCAACTTGATGATATGCTTGGGTTGAAATTTGACCAAGAAATAGAGGAAAGAATTACCCTTATGCTACATAAAAGGTTGAAGATGGAACAAAGTATCCTCGTTGTACTTGATGATGAATGGAAGCAAAATGATTTGGAAATGATAGGAATTCCAAGCATTGAAGATCGCATATGA

Protein sequence

VCEVTRNVENIESGVQNWLTKTDSIIEKSETFLNNLSQQVVKLVDEVVEMKMREILIEFPISSKAKTSDFINFESRKPNIDKIIGSLMDDNVHEIGVYGMRGISRIVMEEKLFDEVVISTIRQLDDMLGLKFDQEIEERITLMLHKRLKMEQSILVVLDDEWKQNDLEMIGIPSIEDRI
Homology
BLAST of Bhi06G000803 vs. TAIR 10
Match: AT4G27190.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 45.1 bits (105), Expect = 7.1e-05
Identity = 48/205 (23.41%), Postives = 90/205 (43.90%), Query Frame = 0

Query: 15  VQNWLTKTDSIIEKSETFLN-----------NLSQQVVKLVDEV--VEMKMREILIEFPI 74
           +  W  + + +I K+   L             +S+++VK++DEV  +E    E +    +
Sbjct: 68  LMRWQREAEEVISKARLKLEERVSCGMSLRPRMSRKLVKILDEVKMLEKDGIEFVDMLSV 127

Query: 75  SSKAKTSDFINFES------RKPNIDKIIGSLMDDNVHEIGVYGMRGISRI----VMEEK 134
            S  +  + +   S          + KI   L  +   +IGV+GM G+ +      +  K
Sbjct: 128 ESTPERVEHVPGVSVVHQTMASNMLAKIRDGLTSEKAQKIGVWGMGGVGKTTLVRTLNNK 187

Query: 135 LFDE-------VVISTI-------RQLDDMLGLKFD-----QEIEERITLMLHKRLKMEQ 178
           L +E       +VI  I       R++   +  + D     +E EE++   ++  L  E+
Sbjct: 188 LREEGATQPFGLVIFVIVSKEFDPREVQKQIAERLDIDTQMEESEEKLARRIYVGLMKER 247

BLAST of Bhi06G000803 vs. TAIR 10
Match: AT5G43730.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 44.3 bits (103), Expect = 1.2e-04
Identity = 48/167 (28.74%), Postives = 81/167 (48.50%), Query Frame = 0

Query: 35  NLSQQVVKLVDEVVEM--KMREILIEFPISSKAKTSDFINFESRKPNIDKIIG----SLM 94
           N   +V+K ++EV E+  K    ++   I  KA+             +D ++G    SL+
Sbjct: 113 NYGGKVMKNLEEVKELLSKKNFEVVAQKIIPKAEKKHI----QTTVGLDTMVGIAWESLI 172

Query: 95  DDNVHEIGVYGMRGISRIVMEEKL----------FDE---VVISTIRQL----DDMLG-- 154
           DD +  +G+YGM GI +  + E L          FD    VV+S   QL    D +LG  
Sbjct: 173 DDEIRTLGLYGMGGIGKTTLLESLNNKFVELESEFDVVIWVVVSKDFQLEGIQDQILGRL 232

Query: 155 ---LKFDQEIEERITLMLHKRLKMEQSILVVLDDEWKQNDLEMIGIP 174
               ++++E E +   +++  LK ++ +L +LDD W + DL  IG+P
Sbjct: 233 RPDKEWERETESKKASLINNNLKRKKFVL-LLDDLWSEVDLIKIGVP 274

BLAST of Bhi06G000803 vs. TAIR 10
Match: AT1G12210.1 (RPS5-like 1 )

HSP 1 Score: 43.5 bits (101), Expect = 2.1e-04
Identity = 41/160 (25.62%), Postives = 77/160 (48.12%), Query Frame = 0

Query: 37  SQQVVKLVDEVVEMKMREILIEFPISSKAKTSDFINFESRKPNIDKIIGSLMDDNVHEIG 96
           SQ V  +V E   +   E   E PI S     D +        +DK+   LM+D V  +G
Sbjct: 132 SQGVFDIVTEAAPIAEVE---ELPIQSTIVGQDSM--------LDKVWNCLMEDKVWIVG 191

Query: 97  VYGMRGISRIVMEEKL----------FDEVV------ISTIRQLDDMLGLK-------FD 156
           +YGM G+ +  +  ++          FD V+       +T+ ++   +G K       +D
Sbjct: 192 LYGMGGVGKTTLLTQINNKFSKLGGGFDVVIWVVVSKNATVHKIQKSIGEKLGLVGKNWD 251

Query: 157 QEIEERITLMLHKRLKMEQSILVVLDDEWKQNDLEMIGIP 174
           ++ + +  L +H  L+ ++ +L +LDD W++ +L++IG+P
Sbjct: 252 EKNKNQRALDIHNVLRRKKFVL-LLDDIWEKVELKVIGVP 279

BLAST of Bhi06G000803 vs. TAIR 10
Match: AT5G43740.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 42.0 bits (97), Expect = 6.0e-04
Identity = 39/170 (22.94%), Postives = 81/170 (47.65%), Query Frame = 0

Query: 35  NLSQQVVKLVDEVVEMKMREILIEFPISSKAKTSDFINFESRK-----PNIDKII----G 94
           N  ++V K+++EV E+  ++         +    + I+   +K       +DK++     
Sbjct: 112 NYGEKVSKMLEEVKELLSKK-------DFRMVAQEIIHKVEKKLIQTTVGLDKLVEMAWS 171

Query: 95  SLMDDNVHEIGVYGMRGISRIVMEEKL----------FDEVVISTIRQ-------LDDML 154
           SLM+D +  +G+YGM G+ +  + E L          FD V+   + +        D +L
Sbjct: 172 SLMNDEIGTLGLYGMGGVGKTTLLESLNNKFVELESEFDVVIWVVVSKDFQFEGIQDQIL 231

Query: 155 G-----LKFDQEIEERITLMLHKRLKMEQSILVVLDDEWKQNDLEMIGIP 174
           G      ++++E E +   +++  L+ ++ +L +LDD W + D+  IG+P
Sbjct: 232 GRLRSDKEWERETESKKASLIYNNLERKKFVL-LLDDLWSEVDMTKIGVP 273

BLAST of Bhi06G000803 vs. TAIR 10
Match: AT5G43740.2 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 42.0 bits (97), Expect = 6.0e-04
Identity = 39/170 (22.94%), Postives = 81/170 (47.65%), Query Frame = 0

Query: 35  NLSQQVVKLVDEVVEMKMREILIEFPISSKAKTSDFINFESRK-----PNIDKII----G 94
           N  ++V K+++EV E+  ++         +    + I+   +K       +DK++     
Sbjct: 112 NYGEKVSKMLEEVKELLSKK-------DFRMVAQEIIHKVEKKLIQTTVGLDKLVEMAWS 171

Query: 95  SLMDDNVHEIGVYGMRGISRIVMEEKL----------FDEVVISTIRQ-------LDDML 154
           SLM+D +  +G+YGM G+ +  + E L          FD V+   + +        D +L
Sbjct: 172 SLMNDEIGTLGLYGMGGVGKTTLLESLNNKFVELESEFDVVIWVVVSKDFQFEGIQDQIL 231

Query: 155 G-----LKFDQEIEERITLMLHKRLKMEQSILVVLDDEWKQNDLEMIGIP 174
           G      ++++E E +   +++  L+ ++ +L +LDD W + D+  IG+P
Sbjct: 232 GRLRSDKEWERETESKKASLIYNNLERKKFVL-LLDDLWSEVDMTKIGVP 273

BLAST of Bhi06G000803 vs. ExPASy Swiss-Prot
Match: Q9T048 (Disease resistance protein At4g27190 OS=Arabidopsis thaliana OX=3702 GN=At4g27190 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 1.0e-03
Identity = 48/205 (23.41%), Postives = 90/205 (43.90%), Query Frame = 0

Query: 15  VQNWLTKTDSIIEKSETFLN-----------NLSQQVVKLVDEV--VEMKMREILIEFPI 74
           +  W  + + +I K+   L             +S+++VK++DEV  +E    E +    +
Sbjct: 68  LMRWQREAEEVISKARLKLEERVSCGMSLRPRMSRKLVKILDEVKMLEKDGIEFVDMLSV 127

Query: 75  SSKAKTSDFINFES------RKPNIDKIIGSLMDDNVHEIGVYGMRGISRI----VMEEK 134
            S  +  + +   S          + KI   L  +   +IGV+GM G+ +      +  K
Sbjct: 128 ESTPERVEHVPGVSVVHQTMASNMLAKIRDGLTSEKAQKIGVWGMGGVGKTTLVRTLNNK 187

Query: 135 LFDE-------VVISTI-------RQLDDMLGLKFD-----QEIEERITLMLHKRLKMEQ 178
           L +E       +VI  I       R++   +  + D     +E EE++   ++  L  E+
Sbjct: 188 LREEGATQPFGLVIFVIVSKEFDPREVQKQIAERLDIDTQMEESEEKLARRIYVGLMKER 247

BLAST of Bhi06G000803 vs. ExPASy TrEMBL
Match: A0A1S3B439 (probable disease resistance protein At4g27220 OS=Cucumis melo OX=3656 GN=LOC103485808 PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 7.1e-53
Identity = 135/224 (60.27%), Postives = 147/224 (65.62%), Query Frame = 0

Query: 1   VCEVTRNVENIESGVQNWLTKTDSIIEKSETFLNNLSQQ----------------VVKLV 60
           V E  RN ENIESGVQNWLTK DSIIEKSET LNNL+QQ                 VKL 
Sbjct: 54  VNEARRNAENIESGVQNWLTKADSIIEKSETLLNNLAQQDGLCLNLVQRHKLSRKTVKLG 113

Query: 61  DEVVEMK---------MREILIEFPI-SSKAKTSDFINFESRKPNIDKIIGSLMDDNVHE 120
           DEVVE+K          R  L+E  + SSKAKTSDF+NFESRKP IDKIIG+LMDDNVH+
Sbjct: 114 DEVVEIKNEGNFDRVSYRVALLEVELESSKAKTSDFVNFESRKPTIDKIIGALMDDNVHK 173

Query: 121 IGVYGMRG---------ISRIVMEEKLFDEVVISTIR----------QLDDMLGLKFDQE 180
           IGVYGM G         IS++ ME KLFDEVV STI           QL D LGLKFDQE
Sbjct: 174 IGVYGMGGVGKTMLVKEISKLAMERKLFDEVVTSTISQTPDIKRIQGQLGDKLGLKFDQE 233

BLAST of Bhi06G000803 vs. ExPASy TrEMBL
Match: A0A5A7UCR2 (Putative disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold55G001990 PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 7.1e-53
Identity = 135/224 (60.27%), Postives = 147/224 (65.62%), Query Frame = 0

Query: 1   VCEVTRNVENIESGVQNWLTKTDSIIEKSETFLNNLSQQ----------------VVKLV 60
           V E  RN ENIESGVQNWLTK DSIIEKSET LNNL+QQ                 VKL 
Sbjct: 54  VNEARRNAENIESGVQNWLTKADSIIEKSETLLNNLAQQDGLCLNLVQRHKLSRKTVKLG 113

Query: 61  DEVVEMK---------MREILIEFPI-SSKAKTSDFINFESRKPNIDKIIGSLMDDNVHE 120
           DEVVE+K          R  L+E  + SSKAKTSDF+NFESRKP IDKIIG+LMDDNVH+
Sbjct: 114 DEVVEIKNEGNFDRVSYRVALLEVELESSKAKTSDFVNFESRKPTIDKIIGALMDDNVHK 173

Query: 121 IGVYGMRG---------ISRIVMEEKLFDEVVISTIR----------QLDDMLGLKFDQE 180
           IGVYGM G         IS++ ME KLFDEVV STI           QL D LGLKFDQE
Sbjct: 174 IGVYGMGGVGKTMLVKEISKLAMERKLFDEVVTSTISQTPDIKRIQGQLGDKLGLKFDQE 233

BLAST of Bhi06G000803 vs. ExPASy TrEMBL
Match: A0A5D3DFP8 (Putative disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold333G00910 PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 7.1e-53
Identity = 135/224 (60.27%), Postives = 147/224 (65.62%), Query Frame = 0

Query: 1   VCEVTRNVENIESGVQNWLTKTDSIIEKSETFLNNLSQQ----------------VVKLV 60
           V E  RN ENIESGVQNWLTK DSIIEKSET LNNL+QQ                 VKL 
Sbjct: 54  VNEARRNAENIESGVQNWLTKADSIIEKSETLLNNLAQQDGLCLNLVQRHKLSRKTVKLG 113

Query: 61  DEVVEMK---------MREILIEFPI-SSKAKTSDFINFESRKPNIDKIIGSLMDDNVHE 120
           DEVVE+K          R  L+E  + SSKAKTSDF+NFESRKP IDKIIG+LMDDNVH+
Sbjct: 114 DEVVEIKNEGNFDRVSYRVALLEVELESSKAKTSDFVNFESRKPTIDKIIGALMDDNVHK 173

Query: 121 IGVYGMRG---------ISRIVMEEKLFDEVVISTIR----------QLDDMLGLKFDQE 180
           IGVYGM G         IS++ ME KLFDEVV STI           QL D LGLKFDQE
Sbjct: 174 IGVYGMGGVGKTMLVKEISKLAMERKLFDEVVTSTISQTPDIKRIQGQLGDKLGLKFDQE 233

BLAST of Bhi06G000803 vs. ExPASy TrEMBL
Match: A0A0A0LN08 (NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G096930 PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 3.5e-44
Identity = 119/221 (53.85%), Postives = 138/221 (62.44%), Query Frame = 0

Query: 1   VCEVTRNVENIESGVQNWLTKTDSIIEKSETFLNNLSQQ----------------VVKLV 60
           V E  RN E IESGVQ+WLTK DSIIE+SET L NLS+Q                 VKL 
Sbjct: 54  VNEAIRNAEKIESGVQSWLTKVDSIIERSETLLKNLSEQGGLCLNLVQRHQLSRKAVKLA 113

Query: 61  DEVVEMKMR--------EILIEFPISSKAKTSDFINFESRKPNIDKIIGSLMDDNVHEIG 120
           +EVV +K+          + +    SSKAK SDF++FESRKP IDKII +LMDDNVH IG
Sbjct: 114 EEVVVIKIEGNFDKVSSPVALSEVESSKAKNSDFVDFESRKPTIDKIIAALMDDNVHTIG 173

Query: 121 VYGMRG---------ISRIVMEEKLFDEVVISTIR----------QLDDMLGLKFDQEIE 178
           VYGM G         IS++ ME+KLFDEV+ ST+           QL D LGL+F+QE E
Sbjct: 174 VYGMGGVGKTMLVQEISKLAMEQKLFDEVITSTVSQTPDLRRIQGQLGDKLGLRFEQETE 233

BLAST of Bhi06G000803 vs. ExPASy TrEMBL
Match: A0A5A7VDT3 (Putative disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00980 PE=4 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 1.1e-29
Identity = 93/219 (42.47%), Postives = 123/219 (56.16%), Query Frame = 0

Query: 6   RNVENIESGVQNWLTKTDSIIEKSETFLNN-----------------LSQQVVKLVDEVV 65
           RN E+I+  V+ WL K D  + +S+  L N                 LS++V K+  EV+
Sbjct: 59  RNAEDIKPAVEEWLKKVDDFVRESDEILANEGGHGRLCSTNLVHRHKLSRKVSKMAYEVL 118

Query: 66  EMKMR---------EILIEFPISSKAKTSDFINFESRKPNIDKIIGSLMDDNVHEIGVYG 125
           EMK           + +I     S  K  DF++F+SRKP + +I+ +L +DNVH IGV+G
Sbjct: 119 EMKNEGESFDTVSYKNVIPSADCSLPKVPDFLDFDSRKPIVKQIMDALSEDNVHRIGVHG 178

Query: 126 M---------RGISRIVMEEKLFDEVVISTIR----------QLDDMLGLKFDQEIEERI 180
           M         +GI R ++E K FDEVV+STI           QL D LGLKF QE  E  
Sbjct: 179 MGGVGKTMLVKGIIRKIIESKFFDEVVMSTISQTPDFKSIQGQLADKLGLKFQQETLEGR 238

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G27190.17.1e-0523.41NB-ARC domain-containing disease resistance protein [more]
AT5G43730.11.2e-0428.74Disease resistance protein (CC-NBS-LRR class) family [more]
AT1G12210.12.1e-0425.63RPS5-like 1 [more]
AT5G43740.16.0e-0422.94Disease resistance protein (CC-NBS-LRR class) family [more]
AT5G43740.26.0e-0422.94Disease resistance protein (CC-NBS-LRR class) family [more]
Match NameE-valueIdentityDescription
Q9T0481.0e-0323.41Disease resistance protein At4g27190 OS=Arabidopsis thaliana OX=3702 GN=At4g2719... [more]
Match NameE-valueIdentityDescription
A0A1S3B4397.1e-5360.27probable disease resistance protein At4g27220 OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A5A7UCR27.1e-5360.27Putative disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A5D3DFP87.1e-5360.27Putative disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A0A0LN083.5e-4453.85NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G096930 PE=4... [more]
A0A5A7VDT31.1e-2942.47Putative disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 33..53
NoneNo IPR availablePANTHERPTHR33463:SF100RPP4C3coord: 4..173
NoneNo IPR availablePANTHERPTHR33463FAMILY NOT NAMEDcoord: 4..173

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi06M000803Bhi06M000803mRNA