Bhi06G000667 (gene) Wax gourd (B227) v1

Overview


Name	Bhi06G000667
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Cystatin
Location	chr6: 18629274 .. 18629975 (-)
RNA-Seq Expression	Bhi06G000667
Synteny	Bhi06G000667

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAACAACTATAAATATCGAACTCCTTAACAATATTGAAAAGCATACCAGTCTTACAAAGTATATATCTATTCAAAAGGGTAATAGCAATGAGTGTTAAGGATGTTTCCAGCTTTGATCCAGTGCCAATTAAGGATATTGATAGCGAAGAGATTCAAGGATTTGGACGGTTTGCAGCAAGAGAAAGCGGTTTGGAGTTCATAAAAGTGCTGAACGGTTTGGAACAAGGACGACGATTGGGTCAAATACTTTTCACTCTTGTGTTGGCAGCTAAAAACTCCGATGGAATTATTTGGACTTATATAGCTAAGGTGTTTTATAATCAACGAGCCGCATTACAACTCGTAGAGTTCAAGCCTGTACTTCCCGAAAAATAAGCACTGCAATGGGTCCCTACTTAACAATATGCCTTATATATTGTAGTATTGTCATCCATCTATAAATTGACATGCATATATACAAAATAAAATAAAAATACTTATGATCTATTTCTCATCTTTATCCATCTTTATCTACCTATATAGGGTAGGGGTGTTCATTGAAACGGCTAAAACTGAATCGAATGCTAAACTAAATCGAAACCGAATTAATGTGGTTCGGTTCAGTTTGAGAAATACCCTATATTATAGGTTGTTTCTCTCTCCTTCATTCTCTCAAATTAATCGAGATTCTCTCCAATTAATTTTATGAAAGTAACAATTTGG

mRNA sequence

CAACAACTATAAATATCGAACTCCTTAACAATATTGAAAAGCATACCAGTCTTACAAAGTATATATCTATTCAAAAGGGTAATAGCAATGAGTGTTAAGGATGTTTCCAGCTTTGATCCAGTGCCAATTAAGGATATTGATAGCGAAGAGATTCAAGGATTTGGACGGTTTGCAGCAAGAGAAAGCGGTTTGGAGTTCATAAAAGTGCTGAACGGTTTGGAACAAGGACGACGATTGGGTCAAATACTTTTCACTCTTGTGTTGGCAGCTAAAAACTCCGATGGAATTATTTGGACTTATATAGCTAAGGTGTTTTATAATCAACGAGCCGCATTACAACTCGTAGAGTTCAAGCCTGTACTTCCCGAAAAATAAGCACTGCAATGGGTCCCTACTTAACAATATGCCTTATATATTGTAGTATTGTCATCCATCTATAAATTGACATGCATATATACAAAATAAAATAAAAATACTTATGATCTATTTCTCATCTTTATCCATCTTTATCTACCTATATAGGGTAGGGGTGTTCATTGAAACGGCTAAAACTGAATCGAATGCTAAACTAAATCGAAACCGAATTAATGTGGTTCGGTTCAGTTTGAGAAATACCCTATATTATAGGTTGTTTCTCTCTCCTTCATTCTCTCAAATTAATCGAGATTCTCTCCAATTAATTTTATGAAAGTAACAATTTGG

Coding sequence (CDS)

ATGAGTGTTAAGGATGTTTCCAGCTTTGATCCAGTGCCAATTAAGGATATTGATAGCGAAGAGATTCAAGGATTTGGACGGTTTGCAGCAAGAGAAAGCGGTTTGGAGTTCATAAAAGTGCTGAACGGTTTGGAACAAGGACGACGATTGGGTCAAATACTTTTCACTCTTGTGTTGGCAGCTAAAAACTCCGATGGAATTATTTGGACTTATATAGCTAAGGTGTTTTATAATCAACGAGCCGCATTACAACTCGTAGAGTTCAAGCCTGTACTTCCCGAAAAATAA

Protein sequence

MSVKDVSSFDPVPIKDIDSEEIQGFGRFAARESGLEFIKVLNGLEQGRRLGQILFTLVLAAKNSDGIIWTYIAKVFYNQRAALQLVEFKPVLPEK

Homology

[Close]

BLAST of Bhi06G000667 vs. ExPASy TrEMBL
Match: A0A5A7TFU6 (Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00010 PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 2.1e-27
Identity = 65/100 (65.00%), Postives = 81/100 (81.00%), Query Frame = 0


Query: 1   MSVKDVSSFDPVPIKDIDSEEIQGFGRFAARE------SGLEFIKVLNGLEQGRRLGQIL 60
           MS +D+S+ DPVPIKD+DSEEIQG G FA RE        LEF+KV+NG EQGRR  +I+
Sbjct: 1   MSSEDLSNLDPVPIKDVDSEEIQGLGEFAVREHNEEKGENLEFVKVINGFEQGRRGVRII 60

Query: 61  FTLVLAAKNSDGIIWTYIAKVFYNQRAALQLVEFKPVLPE 95
           FT+VL+AKN +GIIWTY AKVF+NQRAA++L+EF+ VLPE
Sbjct: 61  FTIVLSAKNCNGIIWTYKAKVFFNQRAAVRLLEFEAVLPE 100

[Close]

BLAST of Bhi06G000667 vs. ExPASy TrEMBL
Match: A0A5D3C5B1 (Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00040 PE=4 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.1e-23
Identity = 61/90 (67.78%), Postives = 73/90 (81.11%), Query Frame = 0


Query: 12  VPIKDIDSEEIQGFGRFAARE-------SGLEFIKVLNGLEQGRRLGQILFTLVLAAKNS 71
           VPIKD+DSEEIQGFGRFA R+         LEF+KV+NGLEQGRR  +I++TLVL+AKNS
Sbjct: 15  VPIKDVDSEEIQGFGRFAVRKHNKISTGENLEFVKVINGLEQGRRGFRIVYTLVLSAKNS 74

Query: 72  DGIIWTYIAKVFYNQRAALQLVEFKPVLPE 95
            GIIWTY AKVF+N RAA+ L+EF+ VLPE
Sbjct: 75  CGIIWTYKAKVFFNNRAAVLLLEFEAVLPE 104

[Close]

BLAST of Bhi06G000667 vs. ExPASy TrEMBL
Match: A0A0A0KRZ8 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G487690 PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.0e-21
Identity = 64/103 (62.14%), Postives = 76/103 (73.79%), Query Frame = 0


Query: 1   MSVKDVS--SFDP-VPIKDIDSEEIQGFGRFAARE------SGLEFIKVLNGLEQGRRLG 60
           MS +D+S    DP VPIKD+DSEEI+GFG FA  E        LEF+KV+NGLEQGRR  
Sbjct: 1   MSSEDISIPKVDPAVPIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRGF 60

Query: 61  QILFTLVLAAKNSDGIIWTYIAKVFYNQRAALQLVEFKPVLPE 95
           +I FTLVL+AKNS GIIWTY  KVF NQRA ++L+EF+ VL E
Sbjct: 61  RIDFTLVLSAKNSIGIIWTYKVKVFLNQRAGVRLLEFEAVLNE 103

[Close]

BLAST of Bhi06G000667 vs. ExPASy TrEMBL
Match: A0A0A0KNL2 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G487700 PE=4 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.2e-21
Identity = 58/99 (58.59%), Postives = 72/99 (72.73%), Query Frame = 0


Query: 3   VKDVSSFDPVPIKDIDSEEIQGFGRFAAR-------ESGLEFIKVLNGLEQGRRLGQILF 62
           V  +  +D VPIKD+DSEEIQG G  A R          LEF+KV+NGLE+GRR  QI++
Sbjct: 12  VSKLPDYDTVPIKDVDSEEIQGIGGMAVRMHNETSTGENLEFVKVINGLEKGRRGFQIVY 71

Query: 63  TLVLAAKNSDGIIWTYIAKVFYNQRAALQLVEFKPVLPE 95
           TLVL+AKNS GI+WTY AKV +N RAA++L EF+ VLPE
Sbjct: 72  TLVLSAKNSLGIVWTYKAKVIFNNRAAVRLPEFEAVLPE 110

[Close]

BLAST of Bhi06G000667 vs. ExPASy TrEMBL
Match: A0A5D3DF67 (Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26872G00010 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.8e-10
Identity = 38/65 (58.46%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 1  MSVKDVSSFDPVPIKDIDSEEIQGFGRFAARE------SGLEFIKVLNGLEQGRRLGQIL 60
          MS +D+S+ DPVP KD+DSEEIQGFG FA RE        LEF+KV+NG EQGRR+  + 
Sbjct: 1  MSFEDLSNLDPVPFKDVDSEEIQGFGAFAVREHNEEKGENLEFVKVINGFEQGRRVRLLE 60

The following BLAST results are available for this feature:

BLAST of Bhi06G000667 vs.


Match Name	E-value	Identity	Description

BLAST of Bhi06G000667 vs.


Match Name	E-value	Identity	Description

BLAST of Bhi06G000667 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TFU6	2.1e-27	65.00	Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00010 PE=4 S...	[more]
A0A5D3C5B1	1.1e-23	67.78	Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00040 PE=4 S...	[more]
A0A0A0KRZ8	1.0e-21	62.14	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G487690 PE...	[more]
A0A0A0KNL2	2.2e-21	58.59	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G487700 PE...	[more]
A0A5D3DF67	1.8e-10	58.46	Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26872G00010 PE=...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.10		coord: 4..95 e-value: 9.4E-8 score: 33.9
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 14..92

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi06M000667	Bhi06M000667	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity