Bhi06G000527 (gene) Wax gourd (B227) v1

Overview
NameBhi06G000527
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionProtein kinase APK1B
Locationchr6: 14039582 .. 14043557 (-)
RNA-Seq ExpressionBhi06G000527
SyntenyBhi06G000527
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGGTTTGTAAATTGTTAGTTTCGTTGTCCTTTCCATCAGAGAAGATACGGTATCTGGCCGACTCCTCAGACGACAACCACGAGTCAACTCGTGTCAACCCTTTTACACAAACATTTGCTTTCACTTTTGACCTTTTTTTTTCTCAAATTCCCATTGGCAAATCACCTCACACTTATATTTATATATACACACATACATATTCTCATCTCTCTAATTAGTTCATATCCCCATTTTCAGATCAACTCCACAGAAAAATGGGATCTTGTGTTAGTGTTGTTGCTTTAGAAAACAGAACCCCTTCTTGTGGTACATTCATTCATTATAATTTTGTTCTTCGCTTTTAAACTTCATGAAAATCAGTTCAGTCTTCTTAAATTTGCAGTTTTTTTGCTTTTTTCTTTTCTTTTATTCAGTGAATTCGAATGGTTCGAGCTCCTCTGCCTCTGTCCATCCAAGTCCAAGAACAGAGGACGAGATTCTGCAGTCTCCGAATCTGAAGAGATTTTGCTTTAATGAACTGAAAAAGGCGACGGGAAATTTCCGGCAAGGGAGTATGGTCGGGGAAGGTGGGTTTGGGAGTGTGTTCAAGGGCTGGATCGACCAGCAATCGTTCACCGCCACCAAGCCCGGCTCTGGCATCGCTATTGCTGTCAAAAGACACAATCAAGATGGCTTCCAAGGCCACAATGAATGGTTGGTCAGTATCCAATTACTCCTCACCTTCGTCGATTTTTTGTCCTTTAAAATTGTTTGGCCATCAACTTTCATTTAAAATGATTTAGTCCTTTTATATTTTTAATTTTATAATAATTTAGTCTTAAATTTTAGTGTGTCACGATTTAATCTTGATAACAATTTAATCAAATATGTAGGTTTTATATTTTGTAAAATTATTGTGACTCTAATTAGTTTATCGGTTTATTTATACAATAAATCTCATTAACACTAAATTCTAAATTCTAAATAGGGACTAACTCGTTACGATTTTTAAAATACAATGTCGTTACGATTTTTAAAATACAATGACTAAATTTTACATAGTAAAGTCTAAGGACTAAATTATTACAAAATTGAAAATATATAGAATAAATTGTTACAAATCAAAATTCAAAAACTCAATTATTACTTTTATGAAAGTTTAGGGACGAAAATTGATTTTTAACCAAATTACTATTTAGAGGATGTTTGAGCGAAGTAACTCACCCCATGTTTAGGACACCAATTATAATAGTCATGGTTCCAACTATTATAACTCACAATTAACTACAATATTAATATTTCAAGTCACTTTTTATCCGTGTTCACTCTTTTATTCTCATTTTCTTTATTATAGTGTTTACTATTCCTACTCAATTAAAATAGAACCCAAACTAAAATAATATGCACTATAAACACAAATTACTATAACTTATTGACTATAATAACCAACTCAACGTTCCAAACGCTCTCTTAATGTTTTCATTTAAAAACGAGAAATTCTTAGATGCATTTTGGATAGCACCTATCTTTGTACATTCTACCAAATTCTCTAATCATAATTTGTCATGTGACAAAAAAAATATTTTTTTATTTTTGGTGTGTGGCGAGAATGGAATGCATCATCCAAGTAATTAGTCTAGTAAAATATAGTTTAAAATTTTCAATTATTTGTATTAATGTGGGGGTAATGTTGGAATTTTGTTGATTATAGACGGAAATAAACTATTTGGGGCAGTTGCATCATCCTAATTTGGTGAAACTGATCGGTTATTGCTTGGAGAATGAGGATCAACTTCTTGCTTATGAGTTCATGTCAAGAGGCAGCTTGGACAATCACCTTTTTGGAAGTGAGTCAACCTCCTAAATTCATCTTCCTCCATTCATTCCTTTTATCATTTAGATTTAACTACCACCTTGGTACGAAAAACGTTAAATTATAAATTTAATCTATTTCATCTCTAAATTCTTAGTTTTGTATCTATTAAATCTATCAAGTAAAGTAAGAACAAGTTAGAAAAAGTTTAGAGCATCACTTTTGTTATGGAATCGACAGATAAAAAAATCATAAAACGTAAACAGCATCAAGATTAAGATTGGTTAGATTTTATGTTAATTTCCAAATCAATATAGGAAAATGTTAAGAAAATGAGTAATTTTAATTTCAGGAGGATCTGGGTCTCAACCACTTTCATGGAAGTTGCGAATGAAAATTGCTCTGGATGCAGCTAAGGGATTGGCGTATTTGCATGGCCAAAAAGTCATCCATCGTGATTTCAAATCCTCAAACATCCTGCTTGATGCGGTATATATATATATATAAATTTATAAATTCTAACCATAATATAATATAAATTTGTATACTGTTTATAACGAAGTTTGATGTATTAATTACTAACATATGATCTTGGATCTAATTATAATATATTGTTTTGAATTTAATCACACAGAATTACGATGCCAAAATCTCAGACTTTGGACTGGCTAAGGACGGGCCAGTGGGCAATCAAAGCCATGTTTCCACAAGATGTATGGGTACTTATGGCTATGCAGCTCCCGAATACATGGCCACAGGTATATGCCGCATTTCATTCATTTTTAATACGTTTTTTAGGCGCCTATTTAATAATGTTTTTGTTTCTTGTTCTTTAATTTTTTTTTTTTTAAGAAATTGAAGCGTTTTATAACTATTCAAGATGTTTCTTGTTCCATTCATTTTTAATACGTATTTGAGGTGCTCGTATAATAACGTTCTTGTTTTTGGTGTGTTGTTGTTCTTTAGTTTTTTTTAAATAGAATCGTTTTGTAGATTCATGATGTTTCTTGTTTTTACTTTTTTTTTTCACAAATATGACGTGTCCAAAAATTGTGTAGAAAATCAAAATCTCTTTTGATAACTATTTGGTTTTTAGTTTTATTGAATTTTTTTTAAAAAAATTAACCTAATAAATACTATTCTCCATGTACGTTCCTATATATGGTCATCCACTTTTTCTACCCCAAGTTTTCAAAACTAAAAAACAGTATTTTTCAAAATTTTGTTTTTGGTTTTAGAATTTATTTAAAAATTCAAGTATTTCTTGAAATAGGTAAAAATCATAATAGAGAAATTGAGAACTTAGGAACATAAATTTGAAAATTATAAAACAGAAACAAAACCACTGTTAAATGGGGCTCAAATTTCTTTTTTTCTATTTTTTTTCCTGCGGAACATATATAAATGCGTATTGTTTTATTATTCTTCTTCTAAAACCATACATATGATATAAAAATTTTCTATATTTGATTTTAAAGTTTTATATTCATGTAAAAACTTTACATTTTTCACTCACAAAATAAGAAATAAGAACCAAGAGAGAAGGAGATTTGTTCTTTAACAGAAATGCTACCAAACAAGATATGCATTTGAAATTGCTAAAGTTGAGTTTGGTTTTGAAATTAGGTCATTTGACACCGAAGAGCGACGTGTACAGTTTCGGAGCGGTTTTACTCGAGATATTATGTGGAAGACGAGCATTGGATACAACAAAGCCTCCAAGAGAACAAAATTTGGTAGAATGGGCAAAGCCTAATATTAGCAACAGAAGAATCATGAGGATAATGGACAGTAGAATTGCGGGAGATCAGTGTGGTGTAAAGAAGGCAATCACAACAGCTAAACTTGCTTTTAAATGTCTCTCAGAAGACCCTAAACACAGACCTTCAATGTATCAAGTAGTGACAGATTTGGAGCAACTTCAAGATTTTGAATCTCATTTAATTTAAGGACTCATTAATTAATTTTAGTTACTTGTGCATGATGATCATTTAAATGCAGGATCAATATCATCATTAATTCTCTGCATTTTGATTTTTTTTTTCTTTTTATGTTTAATTTAATGATCATTTCTATTTTGGTTCAACATGTGAAATTATGCATGTAAAATTCAAAATTGAATCCTTTTATGGATAATCGTTATACATTGCATTGGGAGTTTTCTCTCACTTTTATTTTTCTA

mRNA sequence

TTTGGTTTGTAAATTGTTAGTTTCGTTGTCCTTTCCATCAGAGAAGATACGGTATCTGGCCGACTCCTCAGACGACAACCACGAGTCAACTCGTGTCAACCCTTTTACACAAACATTTGCTTTCACTTTTGACCTTTTTTTTTCTCAAATTCCCATTGGCAAATCACCTCACACTTATATTTATATATACACACATACATATTCTCATCTCTCTAATTAGTTCATATCCCCATTTTCAGATCAACTCCACAGAAAAATGGGATCTTGTGTTAGTGTTGTTGCTTTAGAAAACAGAACCCCTTCTTGTGTGAATTCGAATGGTTCGAGCTCCTCTGCCTCTGTCCATCCAAGTCCAAGAACAGAGGACGAGATTCTGCAGTCTCCGAATCTGAAGAGATTTTGCTTTAATGAACTGAAAAAGGCGACGGGAAATTTCCGGCAAGGGAGTATGGTCGGGGAAGGTGGGTTTGGGAGTGTGTTCAAGGGCTGGATCGACCAGCAATCGTTCACCGCCACCAAGCCCGGCTCTGGCATCGCTATTGCTGTCAAAAGACACAATCAAGATGGCTTCCAAGGCCACAATGAATGGTTGACGGAAATAAACTATTTGGGGCAGTTGCATCATCCTAATTTGGTGAAACTGATCGGTTATTGCTTGGAGAATGAGGATCAACTTCTTGCTTATGAGTTCATGTCAAGAGGCAGCTTGGACAATCACCTTTTTGGAAGAGGATCTGGGTCTCAACCACTTTCATGGAAGTTGCGAATGAAAATTGCTCTGGATGCAGCTAAGGGATTGGCGTATTTGCATGGCCAAAAAGTCATCCATCGTGATTTCAAATCCTCAAACATCCTGCTTGATGCGAATTACGATGCCAAAATCTCAGACTTTGGACTGGCTAAGGACGGGCCAGTGGGCAATCAAAGCCATGTTTCCACAAGATGTATGGGTACTTATGGCTATGCAGCTCCCGAATACATGGCCACAGGTCATTTGACACCGAAGAGCGACGTGTACAGTTTCGGAGCGGTTTTACTCGAGATATTATGTGGAAGACGAGCATTGGATACAACAAAGCCTCCAAGAGAACAAAATTTGGTAGAATGGGCAAAGCCTAATATTAGCAACAGAAGAATCATGAGGATAATGGACAGTAGAATTGCGGGAGATCAGTGTGGTGTAAAGAAGGCAATCACAACAGCTAAACTTGCTTTTAAATGTCTCTCAGAAGACCCTAAACACAGACCTTCAATGTATCAAGTAGTGACAGATTTGGAGCAACTTCAAGATTTTGAATCTCATTTAATTTAAGGACTCATTAATTAATTTTAGTTACTTGTGCATGATGATCATTTAAATGCAGGATCAATATCATCATTAATTCTCTGCATTTTGATTTTTTTTTTCTTTTTATGTTTAATTTAATGATCATTTCTATTTTGGTTCAACATGTGAAATTATGCATGTAAAATTCAAAATTGAATCCTTTTATGGATAATCGTTATACATTGCATTGGGAGTTTTCTCTCACTTTTATTTTTCTA

Coding sequence (CDS)

ATGGGATCTTGTGTTAGTGTTGTTGCTTTAGAAAACAGAACCCCTTCTTGTGTGAATTCGAATGGTTCGAGCTCCTCTGCCTCTGTCCATCCAAGTCCAAGAACAGAGGACGAGATTCTGCAGTCTCCGAATCTGAAGAGATTTTGCTTTAATGAACTGAAAAAGGCGACGGGAAATTTCCGGCAAGGGAGTATGGTCGGGGAAGGTGGGTTTGGGAGTGTGTTCAAGGGCTGGATCGACCAGCAATCGTTCACCGCCACCAAGCCCGGCTCTGGCATCGCTATTGCTGTCAAAAGACACAATCAAGATGGCTTCCAAGGCCACAATGAATGGTTGACGGAAATAAACTATTTGGGGCAGTTGCATCATCCTAATTTGGTGAAACTGATCGGTTATTGCTTGGAGAATGAGGATCAACTTCTTGCTTATGAGTTCATGTCAAGAGGCAGCTTGGACAATCACCTTTTTGGAAGAGGATCTGGGTCTCAACCACTTTCATGGAAGTTGCGAATGAAAATTGCTCTGGATGCAGCTAAGGGATTGGCGTATTTGCATGGCCAAAAAGTCATCCATCGTGATTTCAAATCCTCAAACATCCTGCTTGATGCGAATTACGATGCCAAAATCTCAGACTTTGGACTGGCTAAGGACGGGCCAGTGGGCAATCAAAGCCATGTTTCCACAAGATGTATGGGTACTTATGGCTATGCAGCTCCCGAATACATGGCCACAGGTCATTTGACACCGAAGAGCGACGTGTACAGTTTCGGAGCGGTTTTACTCGAGATATTATGTGGAAGACGAGCATTGGATACAACAAAGCCTCCAAGAGAACAAAATTTGGTAGAATGGGCAAAGCCTAATATTAGCAACAGAAGAATCATGAGGATAATGGACAGTAGAATTGCGGGAGATCAGTGTGGTGTAAAGAAGGCAATCACAACAGCTAAACTTGCTTTTAAATGTCTCTCAGAAGACCCTAAACACAGACCTTCAATGTATCAAGTAGTGACAGATTTGGAGCAACTTCAAGATTTTGAATCTCATTTAATTTAA

Protein sequence

MGSCVSVVALENRTPSCVNSNGSSSSASVHPSPRTEDEILQSPNLKRFCFNELKKATGNFRQGSMVGEGGFGSVFKGWIDQQSFTATKPGSGIAIAVKRHNQDGFQGHNEWLTEINYLGQLHHPNLVKLIGYCLENEDQLLAYEFMSRGSLDNHLFGRGSGSQPLSWKLRMKIALDAAKGLAYLHGQKVIHRDFKSSNILLDANYDAKISDFGLAKDGPVGNQSHVSTRCMGTYGYAAPEYMATGHLTPKSDVYSFGAVLLEILCGRRALDTTKPPREQNLVEWAKPNISNRRIMRIMDSRIAGDQCGVKKAITTAKLAFKCLSEDPKHRPSMYQVVTDLEQLQDFESHLI
Homology
BLAST of Bhi06G000527 vs. TAIR 10
Match: AT2G28930.3 (protein kinase 1B )

HSP 1 Score: 444.5 bits (1142), Expect = 7.9e-125
Identity = 216/324 (66.67%), Postives = 266/324 (82.10%), Query Frame = 0

Query: 24  SSSASVHPSPRTEDEILQSPNLKRFCFNELKKATGNFRQGSMVGEGGFGSVFKGWIDQQS 83
           SSS S+  +PRTE EILQSPNLK F F ELK AT NFR  S++GEGGFGSVFKGWID+Q+
Sbjct: 36  SSSVSIRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQT 95

Query: 84  FTATKPGSGIAIAVKRHNQDGFQGHNEWLTEINYLGQLHHPNLVKLIGYCLENEDQLLAY 143
            TA+KPG+G+ IAVK+ NQDG+QGH EWL E+NYLGQ  HPNLVKLIGYCLE+E +LL Y
Sbjct: 96  LTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVY 155

Query: 144 EFMSRGSLDNHLFGRGSGSQPLSWKLRMKIALDAAKGLAYLHGQK--VIHRDFKSSNILL 203
           EFM RGSL+NHLF RGS  QPLSW LR+K+AL AAKGLA+LH  +  VI+RDFK+SNILL
Sbjct: 156 EFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILL 215

Query: 204 DANYDAKISDFGLAKDGPVGNQSHVSTRCMGTYGYAAPEYMATGHLTPKSDVYSFGAVLL 263
           D+ Y+AK+SDFGLAKDGP G++SHVSTR MGTYGYAAPEY+ATGHLT KSDVYS+G VLL
Sbjct: 216 DSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLL 275

Query: 264 EILCGRRALDTTKPPREQNLVEWAKPNISN-RRIMRIMDSRIAGDQCGVKKAITTAKLAF 323
           E+L GRRA+D  +PP EQ LVEWA+P ++N R++ R++D+R+  DQ  +++A   A LA 
Sbjct: 276 EVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQ-DQYSMEEACKVATLAL 335

Query: 324 KCLSEDPKHRPSMYQVVTDLEQLQ 345
           +CL+ + K RP+M +VV+ LE +Q
Sbjct: 336 RCLTFEIKLRPNMNEVVSHLEHIQ 358

BLAST of Bhi06G000527 vs. TAIR 10
Match: AT2G28930.1 (protein kinase 1B )

HSP 1 Score: 443.0 bits (1138), Expect = 2.3e-124
Identity = 218/346 (63.01%), Postives = 272/346 (78.61%), Query Frame = 0

Query: 2   GSCVSVVALENRTPSCVNSNGSSSSASVHPSPRTEDEILQSPNLKRFCFNELKKATGNFR 61
           G C+        + +  +    SSS S+  +PRTE EILQSPNLK F F ELK AT NFR
Sbjct: 22  GCCIGASPKYMSSEANDSLGSKSSSVSIRTNPRTEGEILQSPNLKSFTFAELKAATRNFR 81

Query: 62  QGSMVGEGGFGSVFKGWIDQQSFTATKPGSGIAIAVKRHNQDGFQGHNEWLTEINYLGQL 121
             S++GEGGFGSVFKGWID+Q+ TA+KPG+G+ IAVK+ NQDG+QGH EWL E+NYLGQ 
Sbjct: 82  PDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNYLGQF 141

Query: 122 HHPNLVKLIGYCLENEDQLLAYEFMSRGSLDNHLFGRGSGSQPLSWKLRMKIALDAAKGL 181
            HPNLVKLIGYCLE+E +LL YEFM RGSL+NHLF RGS  QPLSW LR+K+AL AAKGL
Sbjct: 142 SHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGL 201

Query: 182 AYLHGQK--VIHRDFKSSNILLDANYDAKISDFGLAKDGPVGNQSHVSTRCMGTYGYAAP 241
           A+LH  +  VI+RDFK+SNILLD+ Y+AK+SDFGLAKDGP G++SHVSTR MGTYGYAAP
Sbjct: 202 AFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAP 261

Query: 242 EYMATGHLTPKSDVYSFGAVLLEILCGRRALDTTKPPREQNLVEWAKPNISN-RRIMRIM 301
           EY+ATGHLT KSDVYS+G VLLE+L GRRA+D  +PP EQ LVEWA+P ++N R++ R++
Sbjct: 262 EYLATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVI 321

Query: 302 DSRIAGDQCGVKKAITTAKLAFKCLSEDPKHRPSMYQVVTDLEQLQ 345
           D+R+  DQ  +++A   A LA +CL+ + K RP+M +VV+ LE +Q
Sbjct: 322 DNRLQ-DQYSMEEACKVATLALRCLTFEIKLRPNMNEVVSHLEHIQ 366

BLAST of Bhi06G000527 vs. TAIR 10
Match: AT2G28930.2 (protein kinase 1B )

HSP 1 Score: 443.0 bits (1138), Expect = 2.3e-124
Identity = 222/356 (62.36%), Postives = 277/356 (77.81%), Query Frame = 0

Query: 1   MGSCVSV---VALENRTPSCVNSNGS------SSSASVHPSPRTEDEILQSPNLKRFCFN 60
           MG C+S          +P  ++S  +      SSS S+  +PRTE EILQSPNLK F F 
Sbjct: 1   MGICLSAQIKAVSPGASPKYMSSEANDSLGSKSSSVSIRTNPRTEGEILQSPNLKSFTFA 60

Query: 61  ELKKATGNFRQGSMVGEGGFGSVFKGWIDQQSFTATKPGSGIAIAVKRHNQDGFQGHNEW 120
           ELK AT NFR  S++GEGGFGSVFKGWID+Q+ TA+KPG+G+ IAVK+ NQDG+QGH EW
Sbjct: 61  ELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEW 120

Query: 121 LTEINYLGQLHHPNLVKLIGYCLENEDQLLAYEFMSRGSLDNHLFGRGSGSQPLSWKLRM 180
           L E+NYLGQ  HPNLVKLIGYCLE+E +LL YEFM RGSL+NHLF RGS  QPLSW LR+
Sbjct: 121 LAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRL 180

Query: 181 KIALDAAKGLAYLHGQK--VIHRDFKSSNILLDANYDAKISDFGLAKDGPVGNQSHVSTR 240
           K+AL AAKGLA+LH  +  VI+RDFK+SNILLD+ Y+AK+SDFGLAKDGP G++SHVSTR
Sbjct: 181 KVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTR 240

Query: 241 CMGTYGYAAPEYMATGHLTPKSDVYSFGAVLLEILCGRRALDTTKPPREQNLVEWAKPNI 300
            MGTYGYAAPEY+ATGHLT KSDVYS+G VLLE+L GRRA+D  +PP EQ LVEWA+P +
Sbjct: 241 IMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLL 300

Query: 301 SN-RRIMRIMDSRIAGDQCGVKKAITTAKLAFKCLSEDPKHRPSMYQVVTDLEQLQ 345
           +N R++ R++D+R+  DQ  +++A   A LA +CL+ + K RP+M +VV+ LE +Q
Sbjct: 301 ANKRKLFRVIDNRLQ-DQYSMEEACKVATLALRCLTFEIKLRPNMNEVVSHLEHIQ 355

BLAST of Bhi06G000527 vs. TAIR 10
Match: AT1G07570.3 (Protein kinase superfamily protein )

HSP 1 Score: 438.7 bits (1127), Expect = 4.3e-123
Identity = 217/335 (64.78%), Postives = 270/335 (80.60%), Query Frame = 0

Query: 18  VNSNGS-SSSASVHPSPRTEDEILQSPNLKRFCFNELKKATGNFRQGSMVGEGGFGSVFK 77
           + S GS +SS SV PSPRTE EILQSPNLK F F ELK AT NFR  S++GEGGFG VFK
Sbjct: 39  IGSLGSKASSVSVRPSPRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGGFGCVFK 98

Query: 78  GWIDQQSFTATKPGSGIAIAVKRHNQDGFQGHNEWLTEINYLGQLHHPNLVKLIGYCLEN 137
           GWID++S TA++PG+G+ IAVK+ NQDG+QGH EWL E+NYLGQ  H +LVKLIGYCLE+
Sbjct: 99  GWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLED 158

Query: 138 EDQLLAYEFMSRGSLDNHLFGRGSGSQPLSWKLRMKIALDAAKGLAYLHGQ--KVIHRDF 197
           E +LL YEFM RGSL+NHLF RG   QPLSWKLR+K+AL AAKGLA+LH    +VI+RDF
Sbjct: 159 EHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETRVIYRDF 218

Query: 198 KSSNILLDANYDAKISDFGLAKDGPVGNQSHVSTRCMGTYGYAAPEYMATGHLTPKSDVY 257
           K+SNILLD+ Y+AK+SDFGLAKDGP+G++SHVSTR MGT+GYAAPEY+ATGHLT KSDVY
Sbjct: 219 KTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLTTKSDVY 278

Query: 258 SFGAVLLEILCGRRALDTTKPPREQNLVEWAKPNISN-RRIMRIMDSRIAGDQCGVKKAI 317
           SFG VLLE+L GRRA+D  +P  E+NLVEWAKP + N R+I R++D+R+  DQ  +++A 
Sbjct: 279 SFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQ-DQYSMEEAC 338

Query: 318 TTAKLAFKCLSEDPKHRPSMYQVVTDLEQLQDFES 349
             A L+ +CL+ + K RP+M +VV+ LE +Q   +
Sbjct: 339 KVATLSLRCLTTEIKLRPNMSEVVSHLEHIQSLNA 372

BLAST of Bhi06G000527 vs. TAIR 10
Match: AT1G07570.2 (Protein kinase superfamily protein )

HSP 1 Score: 438.0 bits (1125), Expect = 7.4e-123
Identity = 224/359 (62.40%), Postives = 279/359 (77.72%), Query Frame = 0

Query: 1   MGSCVSV-VALENRTPSC------VNSNGS-SSSASVHPSPRTEDEILQSPNLKRFCFNE 60
           MG C+S  V  E+   S       + S GS +SS SV PSPRTE EILQSPNLK F F E
Sbjct: 1   MGICLSAQVKAESSGASTKYDAKDIGSLGSKASSVSVRPSPRTEGEILQSPNLKSFSFAE 60

Query: 61  LKKATGNFRQGSMVGEGGFGSVFKGWIDQQSFTATKPGSGIAIAVKRHNQDGFQGHNEWL 120
           LK AT NFR  S++GEGGFG VFKGWID++S TA++PG+G+ IAVK+ NQDG+QGH EWL
Sbjct: 61  LKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWL 120

Query: 121 TEINYLGQLHHPNLVKLIGYCLENEDQLLAYEFMSRGSLDNHLFGRGSGSQPLSWKLRMK 180
            E+NYLGQ  H +LVKLIGYCLE+E +LL YEFM RGSL+NHLF RG   QPLSWKLR+K
Sbjct: 121 AEVNYLGQFSHRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLK 180

Query: 181 IALDAAKGLAYLHGQ--KVIHRDFKSSNILLDANYDAKISDFGLAKDGPVGNQSHVSTRC 240
           +AL AAKGLA+LH    +VI+RDFK+SNILLD+ Y+AK+SDFGLAKDGP+G++SHVSTR 
Sbjct: 181 VALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRV 240

Query: 241 MGTYGYAAPEYMATGHLTPKSDVYSFGAVLLEILCGRRALDTTKPPREQNLVEWAKPNIS 300
           MGT+GYAAPEY+ATGHLT KSDVYSFG VLLE+L GRRA+D  +P  E+NLVEWAKP + 
Sbjct: 241 MGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLV 300

Query: 301 N-RRIMRIMDSRIAGDQCGVKKAITTAKLAFKCLSEDPKHRPSMYQVVTDLEQLQDFES 349
           N R+I R++D+R+  DQ  +++A   A L+ +CL+ + K RP+M +VV+ LE +Q   +
Sbjct: 301 NKRKIFRVIDNRLQ-DQYSMEEACKVATLSLRCLTTEIKLRPNMSEVVSHLEHIQSLNA 358

BLAST of Bhi06G000527 vs. ExPASy Swiss-Prot
Match: Q65XV8 (Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN=RLCK176 PE=1 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 1.5e-125
Identity = 222/329 (67.48%), Postives = 267/329 (81.16%), Query Frame = 0

Query: 20  SNGSSSSASVHPSPRTEDEILQSPNLKRFCFNELKKATGNFRQGSMVGEGGFGSVFKGWI 79
           SN S S+ASV P+PR+EDEIL++ N+K F FNEL+ AT NFR  S++GEGGFGSVFKGWI
Sbjct: 30  SNSSVSAASVPPTPRSEDEILEAANVKAFAFNELRTATRNFRPDSVLGEGGFGSVFKGWI 89

Query: 80  DQQSFTATKPGSGIAIAVKRHNQDGFQGHNEWLTEINYLGQLHHPNLVKLIGYCLENEDQ 139
           D+++   TKPG+G+ IAVK+ NQ+G QGH EWL E+NYLGQL HP LV+L+GYC+E+E +
Sbjct: 90  DEKTLAPTKPGTGMVIAVKKLNQEGHQGHREWLAEVNYLGQLSHPYLVRLVGYCVEDEQR 149

Query: 140 LLAYEFMSRGSLDNHLFGRGSGSQPLSWKLRMKIALDAAKGLAYLHGQ--KVIHRDFKSS 199
           LL YEFM RGSL+NHLF R +  QPLSW LRMKIAL AAKGLA+LH    KVI+RDFK+S
Sbjct: 150 LLVYEFMPRGSLENHLFRRSTHFQPLSWNLRMKIALGAAKGLAFLHSDKVKVIYRDFKTS 209

Query: 200 NILLDANYDAKISDFGLAKDGPVGNQSHVSTRCMGTYGYAAPEYMATGHLTPKSDVYSFG 259
           N+LLDANYDAK+SDFGLAKDGP G++SHVSTR MGTYGYAAPEY+ATGHLT KSDVYSFG
Sbjct: 210 NVLLDANYDAKLSDFGLAKDGPTGDKSHVSTRVMGTYGYAAPEYLATGHLTTKSDVYSFG 269

Query: 260 AVLLEILCGRRALDTTKPPREQNLVEWAKPNI-SNRRIMRIMDSRIAGDQCGVKKAITTA 319
            VLLE+L GRRALD  +P  E NLVEWA+P + S RRI RI+D+R+ G Q  + KA   A
Sbjct: 270 VVLLEMLSGRRALDKNRPTGEHNLVEWARPYLMSKRRIFRILDARLGG-QYSLAKAQKAA 329

Query: 320 KLAFKCLSEDPKHRPSMYQVVTDLEQLQD 346
            LA +C+S + K+RP+M QVV  LEQLQD
Sbjct: 330 TLALQCISVEAKNRPNMEQVVAVLEQLQD 357

BLAST of Bhi06G000527 vs. ExPASy Swiss-Prot
Match: P46573 (Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 GN=PBL10 PE=1 SV=2)

HSP 1 Score: 443.0 bits (1138), Expect = 3.2e-123
Identity = 222/356 (62.36%), Postives = 277/356 (77.81%), Query Frame = 0

Query: 1   MGSCVSV---VALENRTPSCVNSNGS------SSSASVHPSPRTEDEILQSPNLKRFCFN 60
           MG C+S          +P  ++S  +      SSS S+  +PRTE EILQSPNLK F F 
Sbjct: 1   MGICLSAQIKAVSPGASPKYMSSEANDSLGSKSSSVSIRTNPRTEGEILQSPNLKSFTFA 60

Query: 61  ELKKATGNFRQGSMVGEGGFGSVFKGWIDQQSFTATKPGSGIAIAVKRHNQDGFQGHNEW 120
           ELK AT NFR  S++GEGGFGSVFKGWID+Q+ TA+KPG+G+ IAVK+ NQDG+QGH EW
Sbjct: 61  ELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEW 120

Query: 121 LTEINYLGQLHHPNLVKLIGYCLENEDQLLAYEFMSRGSLDNHLFGRGSGSQPLSWKLRM 180
           L E+NYLGQ  HPNLVKLIGYCLE+E +LL YEFM RGSL+NHLF RGS  QPLSW LR+
Sbjct: 121 LAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRL 180

Query: 181 KIALDAAKGLAYLHGQK--VIHRDFKSSNILLDANYDAKISDFGLAKDGPVGNQSHVSTR 240
           K+AL AAKGLA+LH  +  VI+RDFK+SNILLD+ Y+AK+SDFGLAKDGP G++SHVSTR
Sbjct: 181 KVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTR 240

Query: 241 CMGTYGYAAPEYMATGHLTPKSDVYSFGAVLLEILCGRRALDTTKPPREQNLVEWAKPNI 300
            MGTYGYAAPEY+ATGHLT KSDVYS+G VLLE+L GRRA+D  +PP EQ LVEWA+P +
Sbjct: 241 IMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLL 300

Query: 301 SN-RRIMRIMDSRIAGDQCGVKKAITTAKLAFKCLSEDPKHRPSMYQVVTDLEQLQ 345
           +N R++ R++D+R+  DQ  +++A   A LA +CL+ + K RP+M +VV+ LE +Q
Sbjct: 301 ANKRKLFRVIDNRLQ-DQYSMEEACKVATLALRCLTFEIKLRPNMNEVVSHLEHIQ 355

BLAST of Bhi06G000527 vs. ExPASy Swiss-Prot
Match: Q06548 (Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN=PBL9 PE=1 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 1.0e-121
Identity = 224/359 (62.40%), Postives = 279/359 (77.72%), Query Frame = 0

Query: 1   MGSCVSV-VALENRTPSC------VNSNGS-SSSASVHPSPRTEDEILQSPNLKRFCFNE 60
           MG C+S  V  E+   S       + S GS +SS SV PSPRTE EILQSPNLK F F E
Sbjct: 1   MGICLSAQVKAESSGASTKYDAKDIGSLGSKASSVSVRPSPRTEGEILQSPNLKSFSFAE 60

Query: 61  LKKATGNFRQGSMVGEGGFGSVFKGWIDQQSFTATKPGSGIAIAVKRHNQDGFQGHNEWL 120
           LK AT NFR  S++GEGGFG VFKGWID++S TA++PG+G+ IAVK+ NQDG+QGH EWL
Sbjct: 61  LKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWL 120

Query: 121 TEINYLGQLHHPNLVKLIGYCLENEDQLLAYEFMSRGSLDNHLFGRGSGSQPLSWKLRMK 180
            E+NYLGQ  H +LVKLIGYCLE+E +LL YEFM RGSL+NHLF RG   QPLSWKLR+K
Sbjct: 121 AEVNYLGQFSHRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLK 180

Query: 181 IALDAAKGLAYLHGQ--KVIHRDFKSSNILLDANYDAKISDFGLAKDGPVGNQSHVSTRC 240
           +AL AAKGLA+LH    +VI+RDFK+SNILLD+ Y+AK+SDFGLAKDGP+G++SHVSTR 
Sbjct: 181 VALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRV 240

Query: 241 MGTYGYAAPEYMATGHLTPKSDVYSFGAVLLEILCGRRALDTTKPPREQNLVEWAKPNIS 300
           MGT+GYAAPEY+ATGHLT KSDVYSFG VLLE+L GRRA+D  +P  E+NLVEWAKP + 
Sbjct: 241 MGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLV 300

Query: 301 N-RRIMRIMDSRIAGDQCGVKKAITTAKLAFKCLSEDPKHRPSMYQVVTDLEQLQDFES 349
           N R+I R++D+R+  DQ  +++A   A L+ +CL+ + K RP+M +VV+ LE +Q   +
Sbjct: 301 NKRKIFRVIDNRLQ-DQYSMEEACKVATLSLRCLTTEIKLRPNMSEVVSHLEHIQSLNA 358

BLAST of Bhi06G000527 vs. ExPASy Swiss-Prot
Match: P43293 (Probable serine/threonine-protein kinase PBL11 OS=Arabidopsis thaliana OX=3702 GN=PBL11 PE=1 SV=2)

HSP 1 Score: 425.6 bits (1093), Expect = 5.3e-118
Identity = 209/323 (64.71%), Postives = 257/323 (79.57%), Query Frame = 0

Query: 24  SSSASVHPSPRTEDEILQSPNLKRFCFNELKKATGNFRQGSMVGEGGFGSVFKGWIDQQS 83
           SS+AS    PRTE EILQ+ NLK F  +ELK AT NFR  S+VGEGGFG VFKGWID+ S
Sbjct: 32  SSTASFSYMPRTEGEILQNANLKNFSLSELKSATRNFRPDSVVGEGGFGCVFKGWIDESS 91

Query: 84  FTATKPGSGIAIAVKRHNQDGFQGHNEWLTEINYLGQLHHPNLVKLIGYCLENEDQLLAY 143
              +KPG+GI IAVKR NQ+GFQGH EWL EINYLGQL HPNLVKLIGYCLE E +LL Y
Sbjct: 92  LAPSKPGTGIVIAVKRLNQEGFQGHREWLAEINYLGQLDHPNLVKLIGYCLEEEHRLLVY 151

Query: 144 EFMSRGSLDNHLFGRGSGSQPLSWKLRMKIALDAAKGLAYLHG--QKVIHRDFKSSNILL 203
           EFM+RGSL+NHLF RG+  QPLSW  R+++AL AA+GLA+LH    +VI+RDFK+SNILL
Sbjct: 152 EFMTRGSLENHLFRRGTFYQPLSWNTRVRMALGAARGLAFLHNAQPQVIYRDFKASNILL 211

Query: 204 DANYDAKISDFGLAKDGPVGNQSHVSTRCMGTYGYAAPEYMATGHLTPKSDVYSFGAVLL 263
           D+NY+AK+SDFGLA+DGP+G+ SHVSTR MGT GYAAPEY+ATGHL+ KSDVYSFG VLL
Sbjct: 212 DSNYNAKLSDFGLARDGPMGDNSHVSTRVMGTQGYAAPEYLATGHLSVKSDVYSFGVVLL 271

Query: 264 EILCGRRALDTTKPPREQNLVEWAKPNISN-RRIMRIMDSRIAGDQCGVKKAITTAKLAF 323
           E+L GRRA+D  +P  E NLV+WA+P ++N RR++R+MD R+ G Q  + +A+  A LA 
Sbjct: 272 ELLSGRRAIDKNQPVGEHNLVDWARPYLTNKRRLLRVMDPRLQG-QYSLTRALKIAVLAL 331

Query: 324 KCLSEDPKHRPSMYQVVTDLEQL 344
            C+S D K RP+M ++V  +E+L
Sbjct: 332 DCISIDAKSRPTMNEIVKTMEEL 353

BLAST of Bhi06G000527 vs. ExPASy Swiss-Prot
Match: Q8H186 (Probable serine/threonine-protein kinase PBL1 OS=Arabidopsis thaliana OX=3702 GN=PBL1 PE=1 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 2.9e-116
Identity = 215/353 (60.91%), Postives = 271/353 (76.77%), Query Frame = 0

Query: 1   MGSCVSVVALENRTPSC----VNSNGSSSSASVHPSPRTEDEILQSPNLKRFCFNELKKA 60
           MGSC+S   L   +       ++S  SSSSA+ H   +TE EIL S  +K F FNELK A
Sbjct: 1   MGSCLSSRVLNKSSSGLDDLHLSSCKSSSSATAH---KTEGEILSSTTVKSFSFNELKLA 60

Query: 61  TGNFRQGSMVGEGGFGSVFKGWIDQQSFTATKPGSGIAIAVKRHNQDGFQGHNEWLTEIN 120
           T NFR  S+VGEGGFG VF+GW+D+ + T TK  SG+ IAVKR N DGFQGH EWLTEIN
Sbjct: 61  TRNFRSDSVVGEGGFGCVFRGWLDETTLTPTKSSSGLVIAVKRLNPDGFQGHREWLTEIN 120

Query: 121 YLGQLHHPNLVKLIGYCLENEDQLLAYEFMSRGSLDNHLFGRGSGS-QPLSWKLRMKIAL 180
           YLGQL HPNLVKLIGYCLE+E +LL YEFM +GSL+NHLF  G+   +PLSW LR+K+AL
Sbjct: 121 YLGQLSHPNLVKLIGYCLEDEQRLLVYEFMHKGSLENHLFANGNKDFKPLSWILRIKVAL 180

Query: 181 DAAKGLAYLHGQ--KVIHRDFKSSNILLDANYDAKISDFGLAKDGPVGNQSHVSTRCMGT 240
           DAAKGLA+LH    KVI+RD K+SNILLD++++AK+SDFGLA+DGP+G QS+VSTR MGT
Sbjct: 181 DAAKGLAFLHSDPVKVIYRDIKASNILLDSDFNAKLSDFGLARDGPMGEQSYVSTRVMGT 240

Query: 241 YGYAAPEYMATGHLTPKSDVYSFGAVLLEILCGRRALDTTKPPREQNLVEWAKPNISNRR 300
           +GYAAPEY++TGHL  +SDVYSFG VLLE+LCGR+ALD  +P +EQNLV+WA+P +++RR
Sbjct: 241 FGYAAPEYVSTGHLNARSDVYSFGVVLLELLCGRQALDHNRPAKEQNLVDWARPYLTSRR 300

Query: 301 -IMRIMDSRIAGDQCGVKKAITTAKLAFKCLSEDPKHRPSMYQVVTDLEQLQD 346
            ++ I+D+R+   Q   + A+  A +A +CLS +PK RP+M QVV  L QLQD
Sbjct: 301 KVLLIVDTRL-NSQYKPEGAVRLASIAVQCLSFEPKSRPTMDQVVRALVQLQD 349

BLAST of Bhi06G000527 vs. ExPASy TrEMBL
Match: A0A0A0KSA7 (Protein kinase APK1B, chloroplast OS=Cucumis sativus OX=3659 GN=Csa_5G517150 PE=3 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.4e-182
Identity = 317/347 (91.35%), Postives = 331/347 (95.39%), Query Frame = 0

Query: 1   MGSCVSVVALENRTP-SCVNSNGSSSSASVHPSPRTEDEILQSPNLKRFCFNELKKATGN 60
           MGSCVSV A++NR+P   ++SNGS+SSASVHPSPRTEDEILQSPNLKRFCFNELKKATGN
Sbjct: 1   MGSCVSVPAIDNRSPFHGIDSNGSTSSASVHPSPRTEDEILQSPNLKRFCFNELKKATGN 60

Query: 61  FRQGSMVGEGGFGSVFKGWIDQQSFTATKPGSGIAIAVKRHNQDGFQGHNEWLTEINYLG 120
           FRQ SMVGEGGFGSVFKGWID  S  ATKPGSGIAIAVKRHNQ+GFQGHNEWL EINYLG
Sbjct: 61  FRQRSMVGEGGFGSVFKGWIDHHSLAATKPGSGIAIAVKRHNQEGFQGHNEWLAEINYLG 120

Query: 121 QLHHPNLVKLIGYCLENEDQLLAYEFMSRGSLDNHLFGRGSGSQPLSWKLRMKIALDAAK 180
           QLHHPNLVKLIGYCLENEDQLLAYEFMS+GSLDNHLFGRGSGSQPLSWKLRMKIALDAAK
Sbjct: 121 QLHHPNLVKLIGYCLENEDQLLAYEFMSKGSLDNHLFGRGSGSQPLSWKLRMKIALDAAK 180

Query: 181 GLAYLHGQKVIHRDFKSSNILLDANYDAKISDFGLAKDGPVGNQSHVSTRCMGTYGYAAP 240
           GLAYLHG+KVIHRDFKSSNILLDANYDAKISDFGLAKDGPVGN+SHVSTRCMGTYGYAAP
Sbjct: 181 GLAYLHGKKVIHRDFKSSNILLDANYDAKISDFGLAKDGPVGNESHVSTRCMGTYGYAAP 240

Query: 241 EYMATGHLTPKSDVYSFGAVLLEILCGRRALDTTKPPREQNLVEWAKPNISNRRIMRIMD 300
           EYMATGHLTPKSDVYSFGAVLLEILCGRRALD TK  REQNLVEWAKPNISNRRIMRIMD
Sbjct: 241 EYMATGHLTPKSDVYSFGAVLLEILCGRRALDATKAGREQNLVEWAKPNISNRRIMRIMD 300

Query: 301 SRIAGDQCGVKKAITTAKLAFKCLSEDPKHRPSMYQVVTDLEQLQDF 347
           +RI G +CGVKKAIT AKLAFKCLS+DPKHRPSMYQVVTDLEQLQ+F
Sbjct: 301 NRIEG-ECGVKKAITAAKLAFKCLSDDPKHRPSMYQVVTDLEQLQEF 346

BLAST of Bhi06G000527 vs. ExPASy TrEMBL
Match: A0A1S3CA49 (protein kinase APK1B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103498147 PE=3 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 4.6e-181
Identity = 320/348 (91.95%), Postives = 329/348 (94.54%), Query Frame = 0

Query: 1   MGSCVSVVALEN-RTP-SCVNSNGSSSSASVHPSPRTEDEILQSPNLKRFCFNELKKATG 60
           MGSCVSV A+EN RTP   ++SNGSSSSASVHPSPRTEDEILQSPNLKRFCFNELKKATG
Sbjct: 1   MGSCVSVPAIENRRTPFHGIDSNGSSSSASVHPSPRTEDEILQSPNLKRFCFNELKKATG 60

Query: 61  NFRQGSMVGEGGFGSVFKGWIDQQSFTATKPGSGIAIAVKRHNQDGFQGHNEWLTEINYL 120
           NFRQ SMVGEGGFGSVFKGWID  S  ATKPGSGIAIAVKRHNQDGFQGHNEWL EINYL
Sbjct: 61  NFRQRSMVGEGGFGSVFKGWIDPHSLAATKPGSGIAIAVKRHNQDGFQGHNEWLAEINYL 120

Query: 121 GQLHHPNLVKLIGYCLENEDQLLAYEFMSRGSLDNHLFGRGSGSQPLSWKLRMKIALDAA 180
           GQLHHPNLVKLIGYCLENEDQLLAYEFMS+GSLDNHLFGRGSGSQPLSWKLRMKIALDAA
Sbjct: 121 GQLHHPNLVKLIGYCLENEDQLLAYEFMSKGSLDNHLFGRGSGSQPLSWKLRMKIALDAA 180

Query: 181 KGLAYLHGQKVIHRDFKSSNILLDANYDAKISDFGLAKDGPVGNQSHVSTRCMGTYGYAA 240
           KGLAYLHG+ VIHRDFKSSNILL ANYDAKISDFGLAKDGPVGNQSHVSTRCMGTYGYAA
Sbjct: 181 KGLAYLHGKIVIHRDFKSSNILLGANYDAKISDFGLAKDGPVGNQSHVSTRCMGTYGYAA 240

Query: 241 PEYMATGHLTPKSDVYSFGAVLLEILCGRRALDTTKPPREQNLVEWAKPNISNRRIMRIM 300
           PEYMATGHLTPKSDVYSFGAVLLEILCGRRALD TK  REQNLVEWAKPNISNRRIMRIM
Sbjct: 241 PEYMATGHLTPKSDVYSFGAVLLEILCGRRALDATKTGREQNLVEWAKPNISNRRIMRIM 300

Query: 301 DSRIAGDQCGVKKAITTAKLAFKCLSEDPKHRPSMYQVVTDLEQLQDF 347
           D+RI G +CGVKKAIT AKLAFKCLS+DPKHRPSMYQVVTDLEQLQ+F
Sbjct: 301 DNRIEG-ECGVKKAITAAKLAFKCLSDDPKHRPSMYQVVTDLEQLQEF 347

BLAST of Bhi06G000527 vs. ExPASy TrEMBL
Match: A0A5A7T2F1 (Protein kinase APK1B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold311G00250 PE=3 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 2.8e-178
Identity = 318/348 (91.38%), Postives = 327/348 (93.97%), Query Frame = 0

Query: 1   MGSCVSVVALEN-RTP-SCVNSNGSSSSASVHPSPRTEDEILQSPNLKRFCFNELKKATG 60
           MGSCVSV A+EN RTP   ++SNGSSSSASVHPSPRTEDEILQSPNLKRFCFNELKKATG
Sbjct: 1   MGSCVSVPAIENRRTPFHGIDSNGSSSSASVHPSPRTEDEILQSPNLKRFCFNELKKATG 60

Query: 61  NFRQGSMVGEGGFGSVFKGWIDQQSFTATKPGSGIAIAVKRHNQDGFQGHNEWLTEINYL 120
           NFRQ SMVGEGGFGSVFKGWID  S  ATKPGSGIAIAVKRHNQDGFQGHNEWL EINYL
Sbjct: 61  NFRQRSMVGEGGFGSVFKGWIDPHSLAATKPGSGIAIAVKRHNQDGFQGHNEWLAEINYL 120

Query: 121 GQLHHPNLVKLIGYCLENEDQLLAYEFMSRGSLDNHLFGRGSGSQPLSWKLRMKIALDAA 180
           GQLHHPNLVKLIGYCLENEDQLLAYEFMS+GSLDNHLFGR SGSQPLSWKLRMKIALDAA
Sbjct: 121 GQLHHPNLVKLIGYCLENEDQLLAYEFMSKGSLDNHLFGR-SGSQPLSWKLRMKIALDAA 180

Query: 181 KGLAYLHGQKVIHRDFKSSNILLDANYDAKISDFGLAKDGPVGNQSHVSTRCMGTYGYAA 240
           KGLAYLH + VIHRDFKSSNILL ANYDAKISDFGLAKDGPVGNQSHVSTRCMGTYGYAA
Sbjct: 181 KGLAYLHSKIVIHRDFKSSNILLGANYDAKISDFGLAKDGPVGNQSHVSTRCMGTYGYAA 240

Query: 241 PEYMATGHLTPKSDVYSFGAVLLEILCGRRALDTTKPPREQNLVEWAKPNISNRRIMRIM 300
           PEYMATGHLTPKSDVYSFGAVLLEILCGRRALD TK  REQNLVEWAKPNISNRRIMRIM
Sbjct: 241 PEYMATGHLTPKSDVYSFGAVLLEILCGRRALDATKTGREQNLVEWAKPNISNRRIMRIM 300

Query: 301 DSRIAGDQCGVKKAITTAKLAFKCLSEDPKHRPSMYQVVTDLEQLQDF 347
           D+RI G +CGVKKAIT AKLAFKCLS+DPKHRPSMYQVVTDLEQLQ+F
Sbjct: 301 DNRIEG-ECGVKKAITAAKLAFKCLSDDPKHRPSMYQVVTDLEQLQEF 346

BLAST of Bhi06G000527 vs. ExPASy TrEMBL
Match: A0A6J1HEM7 (receptor-like cytoplasmic kinase 176 OS=Cucurbita moschata OX=3662 GN=LOC111462836 PE=3 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 3.1e-169
Identity = 298/359 (83.01%), Postives = 320/359 (89.14%), Query Frame = 0

Query: 1   MGSCVSVVALENRTP------------SCVNSNGSSSSASVHPSPRTEDEILQSPNLKRF 60
           MGSCVS +A+EN +P                SNGSSSSASVHPSPRTEDEILQS NLKRF
Sbjct: 1   MGSCVS-LAMENGSPYHGKDSSDYEKNGSNESNGSSSSASVHPSPRTEDEILQSSNLKRF 60

Query: 61  CFNELKKATGNFRQGSMVGEGGFGSVFKGWIDQQSFTATKPGSGIAIAVKRHNQDGFQGH 120
           CFNELKKATGNFRQ SMVGEGGFGSVFKGWID  S TATK G+GIAIAVKRHNQDG QGH
Sbjct: 61  CFNELKKATGNFRQRSMVGEGGFGSVFKGWIDDHSLTATKSGTGIAIAVKRHNQDGMQGH 120

Query: 121 NEWLTEINYLGQLHHPNLVKLIGYCLENEDQLLAYEFMSRGSLDNHLFGRGSGSQPLSWK 180
           +EWL EINYLGQLHHPNLVKL+GYCLEN+DQLLAYEFMSRGSLDNHLFGRGS SQPLSWK
Sbjct: 121 SEWLAEINYLGQLHHPNLVKLVGYCLENDDQLLAYEFMSRGSLDNHLFGRGSWSQPLSWK 180

Query: 181 LRMKIALDAAKGLAYLHGQKVIHRDFKSSNILLDANYDAKISDFGLAKDGPVGNQSHVST 240
           LRMKIALDAA+GLAYLH +KVIHRDFKSSNILLDANYDAKISDFGLAKDGP+GNQSHVST
Sbjct: 181 LRMKIALDAARGLAYLHSKKVIHRDFKSSNILLDANYDAKISDFGLAKDGPIGNQSHVST 240

Query: 241 RCMGTYGYAAPEYMATGHLTPKSDVYSFGAVLLEILCGRRALDTTKPPREQNLVEWAKPN 300
           RCMGTYGYAAPEYM+TGHL PKSDVYSFGAVLLEILCGR ALD+TKPP+EQNLV+WA+P 
Sbjct: 241 RCMGTYGYAAPEYMSTGHLMPKSDVYSFGAVLLEILCGRGALDSTKPPKEQNLVDWARPC 300

Query: 301 ISNRRIMRIMDSRIAGDQCGVKKAITTAKLAFKCLSEDPKHRPSMYQVVTDLEQLQDFE 348
           +++RR+ RIMD RI G QC VKKA+  AKLAFKCLSEDPKHRPSMY+VVTDLE+LQD E
Sbjct: 301 VNHRRVSRIMDGRIEG-QCEVKKALRVAKLAFKCLSEDPKHRPSMYEVVTDLEELQDLE 357

BLAST of Bhi06G000527 vs. ExPASy TrEMBL
Match: A0A6J1JSI9 (receptor-like cytoplasmic kinase 176 OS=Cucurbita maxima OX=3661 GN=LOC111487157 PE=3 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 1.2e-168
Identity = 296/359 (82.45%), Postives = 320/359 (89.14%), Query Frame = 0

Query: 1   MGSCVSVVALENRTP------------SCVNSNGSSSSASVHPSPRTEDEILQSPNLKRF 60
           MGSCVS +A+EN +P                +NGS SSASVHPSPRTEDEILQS NLKRF
Sbjct: 1   MGSCVS-LAMENGSPYHGKDSSDYQKNGSNETNGSCSSASVHPSPRTEDEILQSSNLKRF 60

Query: 61  CFNELKKATGNFRQGSMVGEGGFGSVFKGWIDQQSFTATKPGSGIAIAVKRHNQDGFQGH 120
           CFNELKKATGNFRQ SMVGEGGFGSVFKGWID  S TATK G+GIAIAVKRHNQDG QGH
Sbjct: 61  CFNELKKATGNFRQRSMVGEGGFGSVFKGWIDDHSLTATKSGTGIAIAVKRHNQDGMQGH 120

Query: 121 NEWLTEINYLGQLHHPNLVKLIGYCLENEDQLLAYEFMSRGSLDNHLFGRGSGSQPLSWK 180
           +EWL EINYLGQLHHPNLVKL+GYCLEN+DQLLAYEFMSRGSLDNHLFGRGS SQPLSWK
Sbjct: 121 SEWLAEINYLGQLHHPNLVKLVGYCLENDDQLLAYEFMSRGSLDNHLFGRGSWSQPLSWK 180

Query: 181 LRMKIALDAAKGLAYLHGQKVIHRDFKSSNILLDANYDAKISDFGLAKDGPVGNQSHVST 240
           LRMKIALDAA+GLAYLH +KVIHRDFKSSNILLDANYDAKISDFGLAKDGP+GNQSHVST
Sbjct: 181 LRMKIALDAARGLAYLHSKKVIHRDFKSSNILLDANYDAKISDFGLAKDGPIGNQSHVST 240

Query: 241 RCMGTYGYAAPEYMATGHLTPKSDVYSFGAVLLEILCGRRALDTTKPPREQNLVEWAKPN 300
           RCMGTYGYAAPEYM+TGHL PKSDVYSFGAVLLEILCGRRALD+TKPP+EQNLV+WA+P 
Sbjct: 241 RCMGTYGYAAPEYMSTGHLMPKSDVYSFGAVLLEILCGRRALDSTKPPKEQNLVDWARPC 300

Query: 301 ISNRRIMRIMDSRIAGDQCGVKKAITTAKLAFKCLSEDPKHRPSMYQVVTDLEQLQDFE 348
           +++RR++RIMD RI G QC VKKA+  AKLAFKCL EDPKHRPSMY+VVTDLE+LQD E
Sbjct: 301 VNHRRVLRIMDGRIEG-QCEVKKALRVAKLAFKCLWEDPKHRPSMYEVVTDLEELQDLE 357

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G28930.37.9e-12566.67protein kinase 1B [more]
AT2G28930.12.3e-12463.01protein kinase 1B [more]
AT2G28930.22.3e-12462.36protein kinase 1B [more]
AT1G07570.34.3e-12364.78Protein kinase superfamily protein [more]
AT1G07570.27.4e-12362.40Protein kinase superfamily protein [more]
Match NameE-valueIdentityDescription
Q65XV81.5e-12567.48Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
P465733.2e-12362.36Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 G... [more]
Q065481.0e-12162.40Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN... [more]
P432935.3e-11864.71Probable serine/threonine-protein kinase PBL11 OS=Arabidopsis thaliana OX=3702 G... [more]
Q8H1862.9e-11660.91Probable serine/threonine-protein kinase PBL1 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A0A0KSA72.4e-18291.35Protein kinase APK1B, chloroplast OS=Cucumis sativus OX=3659 GN=Csa_5G517150 PE=... [more]
A0A1S3CA494.6e-18191.95protein kinase APK1B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103498147... [more]
A0A5A7T2F12.8e-17891.38Protein kinase APK1B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold311... [more]
A0A6J1HEM73.1e-16983.01receptor-like cytoplasmic kinase 176 OS=Cucurbita moschata OX=3662 GN=LOC1114628... [more]
A0A6J1JSI91.2e-16882.45receptor-like cytoplasmic kinase 176 OS=Cucurbita maxima OX=3661 GN=LOC111487157... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 62..340
e-value: 5.4E-49
score: 166.8
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 35..145
e-value: 4.1E-30
score: 106.0
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 146..349
e-value: 1.9E-63
score: 215.6
NoneNo IPR availablePIRSRPIRSR038172-1PIRSR038172-1coord: 66..244
e-value: 9.3E-16
score: 55.2
NoneNo IPR availablePIRSRPIRSR000556-2PIRSR000556-2coord: 61..267
e-value: 5.4E-25
score: 85.8
NoneNo IPR availablePIRSRPIRSR037993-1PIRSR037993-1coord: 59..267
e-value: 1.7E-9
score: 34.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 17..37
NoneNo IPR availablePANTHERPTHR45621:SF184KINASE 1Bcoord: 19..346
NoneNo IPR availablePANTHERPTHR45621OS01G0588500 PROTEIN-RELATEDcoord: 19..346
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 66..343
e-value: 2.01326E-90
score: 270.298
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 189..201
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 66..98
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 60..351
score: 38.590778
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 35..342

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi06M000527Bhi06M000527mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity