Bhi06G000499 (gene) Wax gourd (B227) v1

Overview
NameBhi06G000499
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionPectate lyase
Locationchr6: 13374470 .. 13378621 (+)
RNA-Seq ExpressionBhi06G000499
SyntenyBhi06G000499
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACTTTCTTCTTCTTTCTTCTTCTTCAAACTTTTTCTTCTTTTGAACTCTAATGGCGGCTTCTTCTTCTCTTTCCTTTCTCTTTCTTCTTTTATCTCTTTCTTTCTTCTTCACTATGTCTGTTTCAACAACAACTTCCAATTCCTCCTTGCATTCTGTTCATCAACATCCGCATCTTGTCGTTCAAGATGTTCAAAGGTACATACATTTTTGTTTCTAATTCTCCTATATATGTGGTCCGCTTCTTCCTACTTTTAAAATATTTACTTTGATGATGTATTTTTTTTTTAGAAAAAGAAAACCATTTTTGTTTCTACATTTTCCATGTTCATTATTTTTTTTAACAAAGTTTTGGCATTAACATCACTCACTAATTATTTTTTTTAATGTAACCATAAATTTATATAAAAGAGTTTCTATTATGAGTAATTTTTGTTTTATAATTTTGTAGATAGAGATTAAAATAATCTTTTTAAAAAATACATGACAAATAGACAATTTGAAGGTAGGGGTGTTCATGAGTTGGATTGAAAGACTTTTTAAACTCAACCTAATTGTTCGGGTTGTAAATTTTTACAACCCTTATTAAAAAATGAACCCAATCCAACCCAACCGTGAAATATTTGGGTTGGGTTAGTTCGGGTTAATTATTTTCATATATTGAATTAAGATTAACAACTCAATTTCAATTTATATAGTGAAAATTTCTGTGCAAAGAAACTGTTGAAGAATAAATTATTAAAAAAAATATTGGATTAAATAAAATAAAATCAATATATGTATAAATAATTGTGTTATATGTAATAAAAAAATGTATTTTAAAGTTAATAATAAATTTAGGTTGGTTCGGGTTATATTAGATGAACCGATGAACCAACCCGACCCATAAAATTTTTATTTATTTGAACTCAACCCAACCCAACCTAAATAAGTTGATAATCCAACCCAAACCGTACGAGTTGGGTTGGGTTGAAAGATTTTTTCGAATCATCGGATTTTTTGAACACTCTTATTTGAAGGTGGAATATAATGACTAAAAGAACAAAGGTTGAAATTCCATGATTTGATATTTTGAAAGGACAAGTAGCTAAAATAAAATCAAAGCAGAGTAATATTTAAAATTTTGGGGAAAATGCTTTTTAGTATAAGTTTTATATTTGGTGTTTAGATTTCAAAACATTATATTTTTATCTTTAAGTTTTGAGTTTAGTTATTTATTAGTAATCTTTACTTTTAAAATGGTAACTAATTTCAATTAGTTAATTTGAAACTATTTATAGTTTAGATTATTGAATTAATTGAAGTAAAAGTCGATATTATTGAAAATTCAAAGATAAAAAAGGTAGCCTTTTATAACCTAGAGATATGACTAAAAATCACAAACAAATTCAATACCCAAAAATGCATTTTCTCATAAACTTTTCTAGTTTTTTATTGGTTCTTATTAAGAGATATTTCTCTTATATTCTGTTAGTAGTATGTTATTAGTAGTTTAAGTCCACCTGTATTTCAGTTGTTGGCTAATTATTTGTCCAACTTAATTTAAGTCCACGTGTATTTTCATCTTAAATCGAATCGAATCAAAGTGAAAATTTTCAAATCAAACCATCTTAAGGGTGGTCACAACTAAACGAAATCAACATATTCAAAGAAAATATATACCAAACTAAATCCTAAATATAGAATTCGATTTAGTTTTCAGTCTTATATATAATATAAATGGTTCAGTTGGATTTTCAATATATATATATATATATTATTGCTATGTAGGAGCATAAATGCATCAAGGAGAAACTTGGGATATTTCTCTTGCGGAACGGGAAACCCAATAGACGATTGCTGGAGATGCGATTCCAATTGGGCAACCAATCGTAAACGCTTAGCCGATTGCGCCATCGGCTTCGGCAAAAACGCCATCGGAGGAAAAAACGGCCGTTTCTACGTTGTCACGGACGCCGGCGACGACGACCCTGTCAACCCACGCCCCGGCACTCTCCGGCACGCCGTCATTCAAAACGAACCCTTGTGGATCATTTTCAAACGTGACATGGTAATCCAACTCACCCAAGAACTCGTAATGAACTCGTTCAAAACAATCGACGGACGTGGGGCCAGCGTCCATGTGGCAGGTGGGCCATGTATAAAAATCCATTACGCCACAAATATTATAATCCACGGCATTCATATTCACGATTGTAAGAGAGGGGGAAACGCTAATATACGGGACTCCCCACAGCATTCTGGGTGGTGGACTGCATCGGACGGTGATGGAGTGTCAATATTAGGAGGAAGAGAAATCTGGATCGATCATTGCTCTCTGTCTAATTGTAATGATGGGTTGATTGATGCGATTCATGGGTCCACTGCTATTACTATATCCAATAATTATATGACACATCATGATAAGGTGATGTTGTTGGGACATAGTGATTCTTATGCACAAGATAAAAATATGCAAGTTACTATTGCTTTTAACCATTTTGGTGAAGGACTTGTTCAGCGAATGCCAAGGTAAATCATCAATTATCTACTTAATTCTTTCTTTTTCATTTTCATGCTCGTGTTTAATTATTTAGGTTTGTTTTATAACTTTTTTTTTTAAATTTTTTTCCTTCTTTTTTTTTTCATTAAAAAAATGGAATGATCTACTTTTTGTAATTGTGTGTTCTTTCTTTTTTTTTTTTATGTAAAAAAAAAAAAATAGAACAAATTTGATAACTGTTTTTATTTTTGAAAATTAAGTCTATTTTATCCACATTTCTTACCATTGAATTTTTAATTTGGAGGTGGAAGTAGTATCTATAGAATTAGTTTTCAAAAACAAAAACCACAAACAAAATGTTTACTAAACACGACCTAAGTTTTTATAAATATTTAAAAAAAAATGAAGATATATCTATTCATAGGTATTTATGTGCATTTTAGAAGATAAAAATTTATAGAACTAAAAACAAGAAAAAAAAAATCTGATTTGGTTGTTTTGAAATATTTATGACTAAGTGAATGTGCATCAAATTTTGATGGGATTTGACATGCATGCAAAATTAGATGGCTAGAAAAAAGATGATAGAGGGGCACATCACATATGCAATGGTTTAAAGAAAAAACAACCAGTTATCTATTGAATATTTGCCATTTGACCATTTTTTTTTTTAAAAAAAAGAAAAATCTAGGAGGCATGATAAAGTGTAGCCTATAACTTCCTTTTTTAAAGGCTGATAACATTAATGTTTTAATATATCGATATTGACTCTCTCTTTTGTTTGATCTAATCCCTTGTTTAACGAGAGTCTATTCTTAAAACGAAACAATTTAAAACGGTTTTGTCCAATTTATATTAGTAAATCAAGACTAGACAACTGTTCAAACTTAACAACAAATTTTCATTGATTACTTTCATTCTCAATTAGACCCATGTTTATGTAATAAAATGTATTTTATTATATTAACTATGCAGATGTAGACATGGTTATTTCCATGTCGTGAACAACGATTACACCCACTGGGAAATGTACGCCATCGGAGGGAGTGCTTCGCCAACGATATACAGCCAAGGGAACAGATTTGCAGCGCCCTACAATCGCTTCCGAAAGGAGGTAACAAAGCACGAGGACGCGCCGGAGAGCGAGTGGCGCCACTGGAATTGGCGCTCCGAGGGCGACATGATGTTGAACGGCGCTTATTTCCGCCAGTCCGGCGCCGGTGCCGCCTCCAGCTATGCACGAGCTAACAGCCTCAGCGCGAGACCCTCCTCTCTTGTTAGCTCTATCACCGTTACCGCCGGCGCCCTCAACTGCCGCAAGGGCTCTCGCTGCTAGTGCTAGTTCTACATTATTTAGTATATCTTTTAGTTAAATTATATTAAAAAAGGTATAATTAGTTCATGAATATCGGGAAATTTTTTCCTGTGTTAATTTATTTTCCTTCCAACATTTTGGGTTTTAGCAAAATGAAAGTGTAATATTTGGTTGAACTCGGATGTTTGTGAGTGTGAAAGCTAATGGGAGAAAATGAGGAATATAGTTATATATTTTGAGTCGAGTCTCCAATTATCTATCACGTTTGTGAAAATTAAAATAATTAAAATTGTATAATTAATTAAGCTAGGAAGAAGGGGAAAATGTAATTTGCTCTTTACCACTCTTCCTTATCT

mRNA sequence

CACTTTCTTCTTCTTTCTTCTTCTTCAAACTTTTTCTTCTTTTGAACTCTAATGGCGGCTTCTTCTTCTCTTTCCTTTCTCTTTCTTCTTTTATCTCTTTCTTTCTTCTTCACTATGTCTGTTTCAACAACAACTTCCAATTCCTCCTTGCATTCTGTTCATCAACATCCGCATCTTGTCGTTCAAGATGTTCAAAGGAGCATAAATGCATCAAGGAGAAACTTGGGATATTTCTCTTGCGGAACGGGAAACCCAATAGACGATTGCTGGAGATGCGATTCCAATTGGGCAACCAATCGTAAACGCTTAGCCGATTGCGCCATCGGCTTCGGCAAAAACGCCATCGGAGGAAAAAACGGCCGTTTCTACGTTGTCACGGACGCCGGCGACGACGACCCTGTCAACCCACGCCCCGGCACTCTCCGGCACGCCGTCATTCAAAACGAACCCTTGTGGATCATTTTCAAACGTGACATGGTAATCCAACTCACCCAAGAACTCGTAATGAACTCGTTCAAAACAATCGACGGACGTGGGGCCAGCGTCCATGTGGCAGGTGGGCCATGTATAAAAATCCATTACGCCACAAATATTATAATCCACGGCATTCATATTCACGATTGTAAGAGAGGGGGAAACGCTAATATACGGGACTCCCCACAGCATTCTGGGTGGTGGACTGCATCGGACGGTGATGGAGTGTCAATATTAGGAGGAAGAGAAATCTGGATCGATCATTGCTCTCTGTCTAATTGTAATGATGGGTTGATTGATGCGATTCATGGGTCCACTGCTATTACTATATCCAATAATTATATGACACATCATGATAAGGTGATGTTGTTGGGACATAGTGATTCTTATGCACAAGATAAAAATATGCAAGTTACTATTGCTTTTAACCATTTTGGTGAAGGACTTGTTCAGCGAATGCCAAGATGTAGACATGGTTATTTCCATGTCGTGAACAACGATTACACCCACTGGGAAATGTACGCCATCGGAGGGAGTGCTTCGCCAACGATATACAGCCAAGGGAACAGATTTGCAGCGCCCTACAATCGCTTCCGAAAGGAGGTAACAAAGCACGAGGACGCGCCGGAGAGCGAGTGGCGCCACTGGAATTGGCGCTCCGAGGGCGACATGATGTTGAACGGCGCTTATTTCCGCCAGTCCGGCGCCGGTGCCGCCTCCAGCTATGCACGAGCTAACAGCCTCAGCGCGAGACCCTCCTCTCTTGTTAGCTCTATCACCGTTACCGCCGGCGCCCTCAACTGCCGCAAGGGCTCTCGCTGCTAGTGCTAGTTCTACATTATTTAGTATATCTTTTAGTTAAATTATATTAAAAAAGGTATAATTAGTTCATGAATATCGGGAAATTTTTTCCTGTGTTAATTTATTTTCCTTCCAACATTTTGGGTTTTAGCAAAATGAAAGTGTAATATTTGGTTGAACTCGGATGTTTGTGAGTGTGAAAGCTAATGGGAGAAAATGAGGAATATAGTTATATATTTTGAGTCGAGTCTCCAATTATCTATCACGTTTGTGAAAATTAAAATAATTAAAATTGTATAATTAATTAAGCTAGGAAGAAGGGGAAAATGTAATTTGCTCTTTACCACTCTTCCTTATCT

Coding sequence (CDS)

ATGGCGGCTTCTTCTTCTCTTTCCTTTCTCTTTCTTCTTTTATCTCTTTCTTTCTTCTTCACTATGTCTGTTTCAACAACAACTTCCAATTCCTCCTTGCATTCTGTTCATCAACATCCGCATCTTGTCGTTCAAGATGTTCAAAGGAGCATAAATGCATCAAGGAGAAACTTGGGATATTTCTCTTGCGGAACGGGAAACCCAATAGACGATTGCTGGAGATGCGATTCCAATTGGGCAACCAATCGTAAACGCTTAGCCGATTGCGCCATCGGCTTCGGCAAAAACGCCATCGGAGGAAAAAACGGCCGTTTCTACGTTGTCACGGACGCCGGCGACGACGACCCTGTCAACCCACGCCCCGGCACTCTCCGGCACGCCGTCATTCAAAACGAACCCTTGTGGATCATTTTCAAACGTGACATGGTAATCCAACTCACCCAAGAACTCGTAATGAACTCGTTCAAAACAATCGACGGACGTGGGGCCAGCGTCCATGTGGCAGGTGGGCCATGTATAAAAATCCATTACGCCACAAATATTATAATCCACGGCATTCATATTCACGATTGTAAGAGAGGGGGAAACGCTAATATACGGGACTCCCCACAGCATTCTGGGTGGTGGACTGCATCGGACGGTGATGGAGTGTCAATATTAGGAGGAAGAGAAATCTGGATCGATCATTGCTCTCTGTCTAATTGTAATGATGGGTTGATTGATGCGATTCATGGGTCCACTGCTATTACTATATCCAATAATTATATGACACATCATGATAAGGTGATGTTGTTGGGACATAGTGATTCTTATGCACAAGATAAAAATATGCAAGTTACTATTGCTTTTAACCATTTTGGTGAAGGACTTGTTCAGCGAATGCCAAGATGTAGACATGGTTATTTCCATGTCGTGAACAACGATTACACCCACTGGGAAATGTACGCCATCGGAGGGAGTGCTTCGCCAACGATATACAGCCAAGGGAACAGATTTGCAGCGCCCTACAATCGCTTCCGAAAGGAGGTAACAAAGCACGAGGACGCGCCGGAGAGCGAGTGGCGCCACTGGAATTGGCGCTCCGAGGGCGACATGATGTTGAACGGCGCTTATTTCCGCCAGTCCGGCGCCGGTGCCGCCTCCAGCTATGCACGAGCTAACAGCCTCAGCGCGAGACCCTCCTCTCTTGTTAGCTCTATCACCGTTACCGCCGGCGCCCTCAACTGCCGCAAGGGCTCTCGCTGCTAG

Protein sequence

MAASSSLSFLFLLLSLSFFFTMSVSTTTSNSSLHSVHQHPHLVVQDVQRSINASRRNLGYFSCGTGNPIDDCWRCDSNWATNRKRLADCAIGFGKNAIGGKNGRFYVVTDAGDDDPVNPRPGTLRHAVIQNEPLWIIFKRDMVIQLTQELVMNSFKTIDGRGASVHVAGGPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHSGWWTASDGDGVSILGGREIWIDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYAQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFAAPYNRFRKEVTKHEDAPESEWRHWNWRSEGDMMLNGAYFRQSGAGAASSYARANSLSARPSSLVSSITVTAGALNCRKGSRC
Homology
BLAST of Bhi06G000499 vs. TAIR 10
Match: AT3G27400.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 662.5 bits (1708), Expect = 2.2e-190
Identity = 312/401 (77.81%), Postives = 347/401 (86.53%), Query Frame = 0

Query: 17  SFFFTMSVSTTTS-NSSLHSVHQHPHLVVQDVQRSI-NASRRNLGYFSCGTGNPIDDCWR 76
           +F   +++  T + +SSL    Q P+LVV DV RS+ NASRR+L Y SC TGNPIDDCWR
Sbjct: 12  AFLLLLTIGNTLAFSSSLPDHVQDPNLVVDDVNRSVFNASRRSLAYLSCRTGNPIDDCWR 71

Query: 77  CDSNWATNRKRLADCAIGFGKNAIGGKNGRFYVVTDAGDDDPVNPRPGTLRHAVIQNEPL 136
           CD NW TNR+RLADCAIGFGKNAIGG+ GR YVVTD  +DDPVNPRPGTLR+AV Q EPL
Sbjct: 72  CDPNWETNRQRLADCAIGFGKNAIGGRKGRIYVVTDPANDDPVNPRPGTLRYAVTQEEPL 131

Query: 137 WIIFKRDMVIQLTQELVMNSFKTIDGRGASVHVAGGPCIKIHYATNIIIHGIHIHDCKRG 196
           WIIFKRDMVI+L +EL++ SFKTIDGRG+SVH+  GPC+KIHYATNIIIHGI+IHDCK G
Sbjct: 132 WIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIIIHGINIHDCKPG 191

Query: 197 GNANIRDSPQHSGWWTASDGDGVSILGGREIWIDHCSLSNCNDGLIDAIHGSTAITISNN 256
               I+D P H+GWW  SDGD V+I GG+ +WIDHCSLSNC+DGLIDAIHGSTAITISNN
Sbjct: 192 SGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAIHGSTAITISNN 251

Query: 257 YMTHHDKVMLLGHSDSYAQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 316
           +MTHHDKVMLLGHSDSY QDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM
Sbjct: 252 HMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 311

Query: 317 YAIGGSASPTIYSQGNRFAAPYNRFRKEVTKHEDAPESEWRHWNWRSEGDMMLNGAYFRQ 376
           YAIGGSASPTIYSQGNRF AP  RF KEVTKHEDAPES+WR WNWRSEGDM+LNGAYFR+
Sbjct: 312 YAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDAPESKWRDWNWRSEGDMLLNGAYFRE 371

Query: 377 SGAGAASSYARANSLSARPSSLVSSITVTAGALNCRKGSRC 416
           SGA A S+YARA+SLSARPSSLV SIT TAG L+CR+G RC
Sbjct: 372 SGAEAPSTYARASSLSARPSSLVGSITTTAGTLSCRRGRRC 412

BLAST of Bhi06G000499 vs. TAIR 10
Match: AT4G24780.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 639.8 bits (1649), Expect = 1.5e-183
Identity = 294/379 (77.57%), Postives = 335/379 (88.39%), Query Frame = 0

Query: 40  PHLVVQDVQRSINAS---RRNLGYFSCGTGNPIDDCWRCDSNWATNRKRLADCAIGFGKN 99
           P  VV++V +SINAS   RR LGY SC TGNPIDDCWRCD +W  +R+RLADCAIGFGKN
Sbjct: 30  PESVVEEVHKSINASVAGRRKLGYLSCTTGNPIDDCWRCDPHWEQHRQRLADCAIGFGKN 89

Query: 100 AIGGKNGRFYVVTDAGDDDPVNPRPGTLRHAVIQNEPLWIIFKRDMVIQLTQELVMNSFK 159
           AIGG++GR YVVTD+G+D+PV+P+PGTLRHAV+Q+EPLWIIF+RDM IQL +EL+MNSFK
Sbjct: 90  AIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEPLWIIFQRDMTIQLKEELIMNSFK 149

Query: 160 TIDGRGASVHVAGGPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHSGWWTASDGDG 219
           TIDGRGASVH++GGPCI I Y TNIIIHGIHIHDCK+GGNA +R SP+H GW T SDGDG
Sbjct: 150 TIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQGGNAMVRSSPRHFGWRTISDGDG 209

Query: 220 VSILGGREIWIDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYAQDKN 279
           VSI GG  +W+DHCS SNC DGLIDAI GSTAIT+SNN+MTHHDKVMLLGHSD+Y++DKN
Sbjct: 210 VSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSNNHMTHHDKVMLLGHSDTYSRDKN 269

Query: 280 MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFAAPY 339
           MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTI SQGNRF AP 
Sbjct: 270 MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPN 329

Query: 340 NRFRKEVTKHEDAPESEWRHWNWRSEGDMMLNGAYFRQSGAGAASSYARANSLSARPSSL 399
            RF KEVTKHEDAPESEW+ WNWRS GD++LNGA+F  SG  A+SSYA+A+SL A+PSSL
Sbjct: 330 IRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFTPSGGAASSSYAKASSLGAKPSSL 389

Query: 400 VSSITVTAGALNCRKGSRC 416
           V  +T T+GALNCRKGSRC
Sbjct: 390 VGPLTSTSGALNCRKGSRC 408

BLAST of Bhi06G000499 vs. TAIR 10
Match: AT4G24780.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 639.8 bits (1649), Expect = 1.5e-183
Identity = 294/379 (77.57%), Postives = 335/379 (88.39%), Query Frame = 0

Query: 40  PHLVVQDVQRSINAS---RRNLGYFSCGTGNPIDDCWRCDSNWATNRKRLADCAIGFGKN 99
           P  VV++V +SINAS   RR LGY SC TGNPIDDCWRCD +W  +R+RLADCAIGFGKN
Sbjct: 30  PESVVEEVHKSINASVAGRRKLGYLSCTTGNPIDDCWRCDPHWEQHRQRLADCAIGFGKN 89

Query: 100 AIGGKNGRFYVVTDAGDDDPVNPRPGTLRHAVIQNEPLWIIFKRDMVIQLTQELVMNSFK 159
           AIGG++GR YVVTD+G+D+PV+P+PGTLRHAV+Q+EPLWIIF+RDM IQL +EL+MNSFK
Sbjct: 90  AIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEPLWIIFQRDMTIQLKEELIMNSFK 149

Query: 160 TIDGRGASVHVAGGPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHSGWWTASDGDG 219
           TIDGRGASVH++GGPCI I Y TNIIIHGIHIHDCK+GGNA +R SP+H GW T SDGDG
Sbjct: 150 TIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQGGNAMVRSSPRHFGWRTISDGDG 209

Query: 220 VSILGGREIWIDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYAQDKN 279
           VSI GG  +W+DHCS SNC DGLIDAI GSTAIT+SNN+MTHHDKVMLLGHSD+Y++DKN
Sbjct: 210 VSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSNNHMTHHDKVMLLGHSDTYSRDKN 269

Query: 280 MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFAAPY 339
           MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTI SQGNRF AP 
Sbjct: 270 MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPN 329

Query: 340 NRFRKEVTKHEDAPESEWRHWNWRSEGDMMLNGAYFRQSGAGAASSYARANSLSARPSSL 399
            RF KEVTKHEDAPESEW+ WNWRS GD++LNGA+F  SG  A+SSYA+A+SL A+PSSL
Sbjct: 330 IRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFTPSGGAASSSYAKASSLGAKPSSL 389

Query: 400 VSSITVTAGALNCRKGSRC 416
           V  +T T+GALNCRKGSRC
Sbjct: 390 VGPLTSTSGALNCRKGSRC 408

BLAST of Bhi06G000499 vs. TAIR 10
Match: AT5G63180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 634.0 bits (1634), Expect = 8.3e-182
Identity = 303/419 (72.32%), Postives = 347/419 (82.82%), Query Frame = 0

Query: 1   MAASSSLSFLFLLLSLSFFFTMSVSTTTSNSSLHSVHQHPHLVVQDVQRSIN--ASRRNL 60
           M  SS LSF  +      F  ++ S   S+         P LVV++V R IN   SRR L
Sbjct: 23  MLNSSYLSFALIFFCCILFSALASSLPVSD---------PELVVEEVHRKINESISRRKL 82

Query: 61  GYFSCGTGNPIDDCWRCDSNWATNRKRLADCAIGFGKNAIGGKNGRFYVVTDAGDDDPVN 120
           G+FSCG+GNPIDDCWRCD +W  NRKRLADC IGFGKNAIGG++G  YVVTD G+DDPVN
Sbjct: 83  GFFSCGSGNPIDDCWRCDKDWEKNRKRLADCGIGFGKNAIGGRDGEIYVVTDPGNDDPVN 142

Query: 121 PRPGTLRHAVIQNEPLWIIFKRDMVIQLTQELVMNSFKTIDGRGASVHVAGGPCIKIHYA 180
           PRPGTLR+AVIQ+EPLWIIFKRDM IQL +EL+MNSFKT+DGRGASVH++GGPCI I Y 
Sbjct: 143 PRPGTLRYAVIQDEPLWIIFKRDMTIQLKEELIMNSFKTLDGRGASVHISGGPCITIQYV 202

Query: 181 TNIIIHGIHIHDCKRGGNANIRDSPQHSGWWTASDGDGVSILGGREIWIDHCSLSNCNDG 240
           TNIIIHG+HIHDCK+GGN  +RDSP+H G+ T SDGDGVSI GG  +W+DHCSLSNCNDG
Sbjct: 203 TNIIIHGLHIHDCKQGGNTYVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDG 262

Query: 241 LIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYAQDKNMQVTIAFNHFGEGLVQRMPRCR 300
           LIDAI GSTAITISNNY+THH+KVMLLGHSD+Y QDKNMQVTIAFNHFGEGLVQRMPRCR
Sbjct: 263 LIDAIRGSTAITISNNYLTHHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCR 322

Query: 301 HGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFAAPYNRFRKEVTKHEDAPESEWRHWN 360
           HGYFHVVNNDYTHWEMYAIGGSA+PTI SQGNRF AP +   KEVTKHEDAPE EWR+WN
Sbjct: 323 HGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPDDSSSKEVTKHEDAPEDEWRNWN 382

Query: 361 WRSEGDMMLNGAYFRQSGAGAA--SSYARANSLSARPSSLVSSITVTAGALNCRKGSRC 416
           WRSEGD++LNGA+F  SGAG A  SSY++A+SL+ARPSS V  IT+ +GAL+C++GS C
Sbjct: 383 WRSEGDLLLNGAFFTYSGAGPAKSSSYSKASSLAARPSSHVGEITIASGALSCKRGSHC 432

BLAST of Bhi06G000499 vs. TAIR 10
Match: AT1G67750.1 (Pectate lyase family protein )

HSP 1 Score: 631.3 bits (1627), Expect = 5.4e-181
Identity = 303/411 (73.72%), Postives = 342/411 (83.21%), Query Frame = 0

Query: 5   SSLSFLFLLLSLSFFFTMSVSTTTSNSSLHSVHQHPHLVVQDVQRSINASRRNLGYFSCG 64
           S  S L L+LS +F  +  VS              P LVVQ+V   INASRRNLG  SCG
Sbjct: 11  SLFSCLLLVLSPTFIASTPVS-------------EPELVVQEVNEKINASRRNLGVLSCG 70

Query: 65  TGNPIDDCWRCDSNWATNRKRLADCAIGFGKNAIGGKNGRFYVVTDAGDDDPVNPRPGTL 124
           TGNPIDDCWRCD  W  NR+RLADCAIGFGK+AIGG++G+ YVVTD+ D D VNP+PGTL
Sbjct: 71  TGNPIDDCWRCDPKWEKNRQRLADCAIGFGKHAIGGRDGKIYVVTDSSDKDVVNPKPGTL 130

Query: 125 RHAVIQNEPLWIIFKRDMVIQLTQELVMNSFKTIDGRGASVHVAGGPCIKIHYATNIIIH 184
           RHAVIQ+EPLWIIF RDMVI+L +EL+MNSFKTIDGRGASVH+AGG CI + Y TNIIIH
Sbjct: 131 RHAVIQDEPLWIIFARDMVIKLKEELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIH 190

Query: 185 GIHIHDCKRGGNANIRDSPQHSGWWTASDGDGVSILGGREIWIDHCSLSNCNDGLIDAIH 244
           G++IHDCKR GNA +RDSP H GW TASDGD VSI GG  +W+DHCSLSNC DGLIDAIH
Sbjct: 191 GVNIHDCKRKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIH 250

Query: 245 GSTAITISNNYMTHHDKVMLLGHSDSYAQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 304
           GSTAITISNNY++HH+KVMLLGHSDSY +DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 251 GSTAITISNNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 310

Query: 305 VNNDYTHWEMYAIGGSASPTIYSQGNRFAAPYNRFRKEVTKHEDAPESEWRHWNWRSEGD 364
           VNNDYTHW+MYAIGGSA+PTI SQGNRF AP +   KEVTK+EDAP S+W+ WNWRSEGD
Sbjct: 311 VNNDYTHWQMYAIGGSAAPTINSQGNRFLAPNDHVFKEVTKYEDAPRSKWKKWNWRSEGD 370

Query: 365 MMLNGAYFRQSGAGAASSYARANSLSARPSSLVSSITVTAGALNCRKGSRC 416
           + LNGA+F  SG GA+SSYA+A+SLSARPSSLV+S+T  AGAL CRKGSRC
Sbjct: 371 LFLNGAFFTPSGGGASSSYAKASSLSARPSSLVASVTSNAGALFCRKGSRC 408

BLAST of Bhi06G000499 vs. ExPASy Swiss-Prot
Match: O24554 (Pectate lyase OS=Zinnia violacea OX=34245 PE=1 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 7.0e-194
Identity = 318/396 (80.30%), Postives = 352/396 (88.89%), Query Frame = 0

Query: 20  FTMSVSTTTSNSSLHSVHQHPHLVVQDVQRSINASRRNLGYFSCGTGNPIDDCWRCDSNW 79
           F  S++  +S+ S     + PH +V +V +SINASRRNLGY SCGTGNPIDDCWRCD NW
Sbjct: 11  FISSLAIASSSPS-----RTPHAIVNEVHKSINASRRNLGYLSCGTGNPIDDCWRCDPNW 70

Query: 80  ATNRKRLADCAIGFGKNAIGGKNGRFYVVTDAGDDDPVNPRPGTLRHAVIQNEPLWIIFK 139
           A NR+RLADCAIGFGKNA+GG+NGR YVVTD G+DDPVNP PGTLR+AVIQ+EPLWIIFK
Sbjct: 71  ANNRQRLADCAIGFGKNAMGGRNGRIYVVTDPGNDDPVNPVPGTLRYAVIQDEPLWIIFK 130

Query: 140 RDMVIQLTQELVMNSFKTIDGRGASVHVAGGPCIKIHYATNIIIHGIHIHDCKRGGNANI 199
           RDMVIQL QELVMNS KTIDGRG +VH+  GPCI IHYA+NIIIHGIHIHDCK+ GN NI
Sbjct: 131 RDMVIQLRQELVMNSHKTIDGRGVNVHIGNGPCITIHYASNIIIHGIHIHDCKQAGNGNI 190

Query: 200 RDSPQHSGWWTASDGDGVSILGGREIWIDHCSLSNCNDGLIDAIHGSTAITISNNYMTHH 259
           R+SP HSGWWT SDGDG+SI   ++IWIDH SLSNC+DGLIDAIHGSTAITISNNYMTHH
Sbjct: 191 RNSPHHSGWWTQSDGDGISIFASKDIWIDHNSLSNCHDGLIDAIHGSTAITISNNYMTHH 250

Query: 260 DKVMLLGHSDSYAQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGG 319
           DKVMLLGHSDSY QDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGG
Sbjct: 251 DKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGG 310

Query: 320 SASPTIYSQGNRFAAPYNRFRKEVTKHEDAPESEWRHWNWRSEGDMMLNGAYFRQSGAGA 379
           SASPTIYSQGNRF AP  RF KEVTKHE+APESEW++WNWRSEGD+MLNGAYFR+SG  A
Sbjct: 311 SASPTIYSQGNRFLAPNTRFDKEVTKHENAPESEWKNWNWRSEGDLMLNGAYFRESGGRA 370

Query: 380 ASSYARANSLSARPSSLVSSITVTAGALNCRKGSRC 416
           ASS+ARA+SLS RPS+LV+S+T +AGAL CRKGSRC
Sbjct: 371 ASSFARASSLSGRPSTLVASMTRSAGALVCRKGSRC 401

BLAST of Bhi06G000499 vs. ExPASy Swiss-Prot
Match: Q9LTZ0 (Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2)

HSP 1 Score: 662.5 bits (1708), Expect = 3.1e-189
Identity = 312/401 (77.81%), Postives = 347/401 (86.53%), Query Frame = 0

Query: 17  SFFFTMSVSTTTS-NSSLHSVHQHPHLVVQDVQRSI-NASRRNLGYFSCGTGNPIDDCWR 76
           +F   +++  T + +SSL    Q P+LVV DV RS+ NASRR+L Y SC TGNPIDDCWR
Sbjct: 12  AFLLLLTIGNTLAFSSSLPDHVQDPNLVVDDVNRSVFNASRRSLAYLSCRTGNPIDDCWR 71

Query: 77  CDSNWATNRKRLADCAIGFGKNAIGGKNGRFYVVTDAGDDDPVNPRPGTLRHAVIQNEPL 136
           CD NW TNR+RLADCAIGFGKNAIGG+ GR YVVTD  +DDPVNPRPGTLR+AV Q EPL
Sbjct: 72  CDPNWETNRQRLADCAIGFGKNAIGGRKGRIYVVTDPANDDPVNPRPGTLRYAVTQEEPL 131

Query: 137 WIIFKRDMVIQLTQELVMNSFKTIDGRGASVHVAGGPCIKIHYATNIIIHGIHIHDCKRG 196
           WIIFKRDMVI+L +EL++ SFKTIDGRG+SVH+  GPC+KIHYATNIIIHGI+IHDCK G
Sbjct: 132 WIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIIIHGINIHDCKPG 191

Query: 197 GNANIRDSPQHSGWWTASDGDGVSILGGREIWIDHCSLSNCNDGLIDAIHGSTAITISNN 256
               I+D P H+GWW  SDGD V+I GG+ +WIDHCSLSNC+DGLIDAIHGSTAITISNN
Sbjct: 192 SGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAIHGSTAITISNN 251

Query: 257 YMTHHDKVMLLGHSDSYAQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 316
           +MTHHDKVMLLGHSDSY QDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM
Sbjct: 252 HMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 311

Query: 317 YAIGGSASPTIYSQGNRFAAPYNRFRKEVTKHEDAPESEWRHWNWRSEGDMMLNGAYFRQ 376
           YAIGGSASPTIYSQGNRF AP  RF KEVTKHEDAPES+WR WNWRSEGDM+LNGAYFR+
Sbjct: 312 YAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDAPESKWRDWNWRSEGDMLLNGAYFRE 371

Query: 377 SGAGAASSYARANSLSARPSSLVSSITVTAGALNCRKGSRC 416
           SGA A S+YARA+SLSARPSSLV SIT TAG L+CR+G RC
Sbjct: 372 SGAEAPSTYARASSLSARPSSLVGSITTTAGTLSCRRGRRC 412

BLAST of Bhi06G000499 vs. ExPASy Swiss-Prot
Match: P24396 (Probable pectate lyase P18 OS=Solanum lycopersicum OX=4081 GN=9612 PE=2 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 6.6e-184
Identity = 310/403 (76.92%), Postives = 343/403 (85.11%), Query Frame = 0

Query: 17  SFFFTMSVSTTTSNSSLHSVHQHPHLVVQDVQRSINAS--RRNLGYFSCGTGNPIDDCWR 76
           + FFT S+        + S+ Q P LVVQDV RSINAS  RRNLGY SCG+GNPID    
Sbjct: 3   TLFFTFSLLLLAPLLVISSI-QDPELVVQDVHRSINASLTRRNLGYLSCGSGNPIDRLLA 62

Query: 77  CDSNWATNRKRLADCAIGFGKNAIGGKNGRFYVVTDAGDDDPVNPRPGTLRHAVIQNEPL 136
                       + CAIGFGKNAIGGKNGR YVVTD+G+DDPVNP+PGTLRHAVIQ+EPL
Sbjct: 63  MQPQLGKKSPAFSYCAIGFGKNAIGGKNGRIYVVTDSGNDDPVNPKPGTLRHAVIQDEPL 122

Query: 137 WIIFKRDMVIQLTQELVMNSFKTIDGRGASVHVAGGPCIKIHYATNIIIHGIHIHDCKRG 196
           WIIFKRDMVIQL QELVMNS+KTIDGRGASVH++GGPCI IH+ +NIIIHGI+IHDCK+ 
Sbjct: 123 WIIFKRDMVIQLKQELVMNSYKTIDGRGASVHISGGPCITIHHTSNIIIHGINIHDCKQS 182

Query: 197 GNANIRDSPQHSGWWTASDGDGVSILGGREIWIDHCSLSNCNDGLIDAIHGSTAITISNN 256
           GN NIRDSP HSGWW  SDGDG+SI GG+ IW+DHCSLSNC+DGLIDAIHGSTAITISNN
Sbjct: 183 GNGNIRDSPNHSGWWDVSDGDGISIFGGKNIWVDHCSLSNCHDGLIDAIHGSTAITISNN 242

Query: 257 YMTHHDKVMLLGHSDSYAQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 316
           Y THHDKVMLLGHSDS+ QDK MQVT+AFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM
Sbjct: 243 YFTHHDKVMLLGHSDSFTQDKGMQVTVAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 302

Query: 317 YAIGGSASPTIYSQGNRFAAPYNRFRKEVTKHEDAPESEWRHWNWRSEGDMMLNGAYFRQ 376
           YAIGGSA+PTI SQGNRF AP  ++RKEVTKHEDAPES+WR WNWRSEGD+MLNGAYFRQ
Sbjct: 303 YAIGGSAAPTINSQGNRFLAPNEKYRKEVTKHEDAPESQWRSWNWRSEGDLMLNGAYFRQ 362

Query: 377 SGAGAASS--YARANSLSARPSSLVSSITVTAGALNCRKGSRC 416
           +GAGA+SS  YARA+SLSARPSSLV SIT  AG +NC+KGSRC
Sbjct: 363 TGAGASSSSTYARASSLSARPSSLVGSITTNAGPVNCKKGSRC 404

BLAST of Bhi06G000499 vs. ExPASy Swiss-Prot
Match: Q9C5M8 (Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2)

HSP 1 Score: 639.8 bits (1649), Expect = 2.1e-182
Identity = 294/379 (77.57%), Postives = 335/379 (88.39%), Query Frame = 0

Query: 40  PHLVVQDVQRSINAS---RRNLGYFSCGTGNPIDDCWRCDSNWATNRKRLADCAIGFGKN 99
           P  VV++V +SINAS   RR LGY SC TGNPIDDCWRCD +W  +R+RLADCAIGFGKN
Sbjct: 30  PESVVEEVHKSINASVAGRRKLGYLSCTTGNPIDDCWRCDPHWEQHRQRLADCAIGFGKN 89

Query: 100 AIGGKNGRFYVVTDAGDDDPVNPRPGTLRHAVIQNEPLWIIFKRDMVIQLTQELVMNSFK 159
           AIGG++GR YVVTD+G+D+PV+P+PGTLRHAV+Q+EPLWIIF+RDM IQL +EL+MNSFK
Sbjct: 90  AIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEPLWIIFQRDMTIQLKEELIMNSFK 149

Query: 160 TIDGRGASVHVAGGPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHSGWWTASDGDG 219
           TIDGRGASVH++GGPCI I Y TNIIIHGIHIHDCK+GGNA +R SP+H GW T SDGDG
Sbjct: 150 TIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQGGNAMVRSSPRHFGWRTISDGDG 209

Query: 220 VSILGGREIWIDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYAQDKN 279
           VSI GG  +W+DHCS SNC DGLIDAI GSTAIT+SNN+MTHHDKVMLLGHSD+Y++DKN
Sbjct: 210 VSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSNNHMTHHDKVMLLGHSDTYSRDKN 269

Query: 280 MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFAAPY 339
           MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTI SQGNRF AP 
Sbjct: 270 MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPN 329

Query: 340 NRFRKEVTKHEDAPESEWRHWNWRSEGDMMLNGAYFRQSGAGAASSYARANSLSARPSSL 399
            RF KEVTKHEDAPESEW+ WNWRS GD++LNGA+F  SG  A+SSYA+A+SL A+PSSL
Sbjct: 330 IRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFTPSGGAASSSYAKASSLGAKPSSL 389

Query: 400 VSSITVTAGALNCRKGSRC 416
           V  +T T+GALNCRKGSRC
Sbjct: 390 VGPLTSTSGALNCRKGSRC 408

BLAST of Bhi06G000499 vs. ExPASy Swiss-Prot
Match: Q93Z25 (Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 1.2e-180
Identity = 303/419 (72.32%), Postives = 347/419 (82.82%), Query Frame = 0

Query: 1   MAASSSLSFLFLLLSLSFFFTMSVSTTTSNSSLHSVHQHPHLVVQDVQRSIN--ASRRNL 60
           M  SS LSF  +      F  ++ S   S+         P LVV++V R IN   SRR L
Sbjct: 23  MLNSSYLSFALIFFCCILFSALASSLPVSD---------PELVVEEVHRKINESISRRKL 82

Query: 61  GYFSCGTGNPIDDCWRCDSNWATNRKRLADCAIGFGKNAIGGKNGRFYVVTDAGDDDPVN 120
           G+FSCG+GNPIDDCWRCD +W  NRKRLADC IGFGKNAIGG++G  YVVTD G+DDPVN
Sbjct: 83  GFFSCGSGNPIDDCWRCDKDWEKNRKRLADCGIGFGKNAIGGRDGEIYVVTDPGNDDPVN 142

Query: 121 PRPGTLRHAVIQNEPLWIIFKRDMVIQLTQELVMNSFKTIDGRGASVHVAGGPCIKIHYA 180
           PRPGTLR+AVIQ+EPLWIIFKRDM IQL +EL+MNSFKT+DGRGASVH++GGPCI I Y 
Sbjct: 143 PRPGTLRYAVIQDEPLWIIFKRDMTIQLKEELIMNSFKTLDGRGASVHISGGPCITIQYV 202

Query: 181 TNIIIHGIHIHDCKRGGNANIRDSPQHSGWWTASDGDGVSILGGREIWIDHCSLSNCNDG 240
           TNIIIHG+HIHDCK+GGN  +RDSP+H G+ T SDGDGVSI GG  +W+DHCSLSNCNDG
Sbjct: 203 TNIIIHGLHIHDCKQGGNTYVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDG 262

Query: 241 LIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYAQDKNMQVTIAFNHFGEGLVQRMPRCR 300
           LIDAI GSTAITISNNY+THH+KVMLLGHSD+Y QDKNMQVTIAFNHFGEGLVQRMPRCR
Sbjct: 263 LIDAIRGSTAITISNNYLTHHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCR 322

Query: 301 HGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFAAPYNRFRKEVTKHEDAPESEWRHWN 360
           HGYFHVVNNDYTHWEMYAIGGSA+PTI SQGNRF AP +   KEVTKHEDAPE EWR+WN
Sbjct: 323 HGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPDDSSSKEVTKHEDAPEDEWRNWN 382

Query: 361 WRSEGDMMLNGAYFRQSGAGAA--SSYARANSLSARPSSLVSSITVTAGALNCRKGSRC 416
           WRSEGD++LNGA+F  SGAG A  SSY++A+SL+ARPSS V  IT+ +GAL+C++GS C
Sbjct: 383 WRSEGDLLLNGAFFTYSGAGPAKSSSYSKASSLAARPSSHVGEITIASGALSCKRGSHC 432

BLAST of Bhi06G000499 vs. ExPASy TrEMBL
Match: A0A1S3C8C5 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103498129 PE=3 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 9.5e-226
Identity = 378/414 (91.30%), Postives = 393/414 (94.93%), Query Frame = 0

Query: 2   AASSSLSFLFLLLSLSFFFTMSVSTTTSNSSLHSVHQHPHLVVQDVQRSINASRRNLGYF 61
           ++SSSL FLFLLLSL FF +MS +   SNSSL  VHQHPHL V++VQRSIN SRRNLGY 
Sbjct: 4   SSSSSLYFLFLLLSLCFFLSMSTAPIPSNSSLPFVHQHPHLAVEEVQRSINGSRRNLGYL 63

Query: 62  SCGTGNPIDDCWRCDSNWATNRKRLADCAIGFGKNAIGGKNGRFYVVTDAGDDDPVNPRP 121
           SCGTGNPIDDCWRCDSNW TNRKRLADCAIGFGKNAIGGKNGRFYVVTDA DDDP+NPRP
Sbjct: 64  SCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVTDARDDDPLNPRP 123

Query: 122 GTLRHAVIQNEPLWIIFKRDMVIQLTQELVMNSFKTIDGRGASVHVAGGPCIKIHYATNI 181
           GTLRHAVIQNEPLWIIFKRDMVIQL QELVMNSFKTIDGRGASVHVAGGPCIKIHYATNI
Sbjct: 124 GTLRHAVIQNEPLWIIFKRDMVIQLEQELVMNSFKTIDGRGASVHVAGGPCIKIHYATNI 183

Query: 182 IIHGIHIHDCKRGGNANIRDSPQHSGWWTASDGDGVSILGGREIWIDHCSLSNCNDGLID 241
           IIHGIHIHDCKRGGNANIRDSPQH+GWWTASDGDGVSILGG++IWIDHCSLSNCNDGLID
Sbjct: 184 IIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDHCSLSNCNDGLID 243

Query: 242 AIHGSTAITISNNYMTHHDKVMLLGHSDSYAQDKNMQVTIAFNHFGEGLVQRMPRCRHGY 301
           AIHGSTAITISNNYMTHHDKVMLLGHSDS+ QDKNMQVTIAFNHFGEGLVQRMPRCRHGY
Sbjct: 244 AIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEGLVQRMPRCRHGY 303

Query: 302 FHVVNNDYTHWEMYAIGGSASPTIYSQGNRFAAPYNRFRKEVTKHEDAPESEWRHWNWRS 361
           FHVVNNDYTHWEMYAIGGSASPTIYSQGNRF APYNRFRKEVTKHEDAPESEWR+WNWRS
Sbjct: 304 FHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDAPESEWRYWNWRS 363

Query: 362 EGDMMLNGAYFRQSGAGAASSYARANSLSARPSSLVSSITVTAGALNCRKGSRC 416
           EGDM+LNGAYFRQSGAGA SSYARA SLSARPSSLV  IT+TAGALNCR+GSRC
Sbjct: 364 EGDMLLNGAYFRQSGAGARSSYARAYSLSARPSSLVGDITLTAGALNCRRGSRC 417

BLAST of Bhi06G000499 vs. ExPASy TrEMBL
Match: A0A0A0KNX3 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_5G517820 PE=3 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 1.4e-224
Identity = 375/414 (90.58%), Postives = 396/414 (95.65%), Query Frame = 0

Query: 2   AASSSLSFLFLLLSLSFFFTMSVSTTTSNSSLHSVHQHPHLVVQDVQRSINASRRNLGYF 61
           ++SSSL FL LLLSLSFF +M+ +   SNSSL  VHQHPHLVV++VQRSIN SRRNLGY 
Sbjct: 6   SSSSSLYFLLLLLSLSFFLSMT-APLPSNSSLPFVHQHPHLVVEEVQRSINGSRRNLGYL 65

Query: 62  SCGTGNPIDDCWRCDSNWATNRKRLADCAIGFGKNAIGGKNGRFYVVTDAGDDDPVNPRP 121
           SCGTGNPIDDCWRCDSNW TNRKRLADCAIGFGKNAIGGKNGRFYVVTD+G+DDP+NPRP
Sbjct: 66  SCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVTDSGNDDPLNPRP 125

Query: 122 GTLRHAVIQNEPLWIIFKRDMVIQLTQELVMNSFKTIDGRGASVHVAGGPCIKIHYATNI 181
           GTLRHAVIQNEPLWIIFKRDMVI+L QELVMNSFKTIDGRGASVH+AGGPCIKIHYATNI
Sbjct: 126 GTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAGGPCIKIHYATNI 185

Query: 182 IIHGIHIHDCKRGGNANIRDSPQHSGWWTASDGDGVSILGGREIWIDHCSLSNCNDGLID 241
           IIHGIHIHDCKRGGNANIRDSPQH+GWWTASDGDGVSILGG++IWIDHCSLSNCNDGLID
Sbjct: 186 IIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDHCSLSNCNDGLID 245

Query: 242 AIHGSTAITISNNYMTHHDKVMLLGHSDSYAQDKNMQVTIAFNHFGEGLVQRMPRCRHGY 301
           AIHGSTAITISNNYMTHHDKVMLLGHSDS+ QDKNMQVTIAFNHFGEGLVQRMPRCRHGY
Sbjct: 246 AIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEGLVQRMPRCRHGY 305

Query: 302 FHVVNNDYTHWEMYAIGGSASPTIYSQGNRFAAPYNRFRKEVTKHEDAPESEWRHWNWRS 361
           FHVVNNDYTHWEMYAIGGSASPTIYSQGNRF APYNRFRKEVTKHEDAPESEWRHWNWRS
Sbjct: 306 FHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDAPESEWRHWNWRS 365

Query: 362 EGDMMLNGAYFRQSGAGAASSYARANSLSARPSSLVSSITVTAGALNCRKGSRC 416
           EGD++LNGAYFRQSGAGA+SSYARA SLSARPSSLV  IT+TAGALNCR+GSRC
Sbjct: 366 EGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGALNCRRGSRC 418

BLAST of Bhi06G000499 vs. ExPASy TrEMBL
Match: A0A5D3CGC7 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold249G00560 PE=3 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 2.4e-221
Identity = 366/394 (92.89%), Postives = 377/394 (95.69%), Query Frame = 0

Query: 22  MSVSTTTSNSSLHSVHQHPHLVVQDVQRSINASRRNLGYFSCGTGNPIDDCWRCDSNWAT 81
           MS +   SNSSL  VHQHPHLVV++VQRSIN SRRNLGY SCGTGNPIDDCWRCDSNW T
Sbjct: 1   MSTAPIPSNSSLPFVHQHPHLVVEEVQRSINGSRRNLGYLSCGTGNPIDDCWRCDSNWET 60

Query: 82  NRKRLADCAIGFGKNAIGGKNGRFYVVTDAGDDDPVNPRPGTLRHAVIQNEPLWIIFKRD 141
           NRKRLADCAIGFGKNAIGGKNGRFYVVTDA DDDP+NPRPGTLRHAVIQNEPLWIIFKRD
Sbjct: 61  NRKRLADCAIGFGKNAIGGKNGRFYVVTDARDDDPLNPRPGTLRHAVIQNEPLWIIFKRD 120

Query: 142 MVIQLTQELVMNSFKTIDGRGASVHVAGGPCIKIHYATNIIIHGIHIHDCKRGGNANIRD 201
           MVIQL QELVMNSFKTIDGRGASVHVAGGPCIKIHYATNIIIHGIHIHDCKRGGNANIRD
Sbjct: 121 MVIQLEQELVMNSFKTIDGRGASVHVAGGPCIKIHYATNIIIHGIHIHDCKRGGNANIRD 180

Query: 202 SPQHSGWWTASDGDGVSILGGREIWIDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDK 261
           SPQH+GWWTASDGDGVSILGG++IWIDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDK
Sbjct: 181 SPQHAGWWTASDGDGVSILGGKQIWIDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDK 240

Query: 262 VMLLGHSDSYAQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA 321
           VMLLGHSDS+ QDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA
Sbjct: 241 VMLLGHSDSFTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA 300

Query: 322 SPTIYSQGNRFAAPYNRFRKEVTKHEDAPESEWRHWNWRSEGDMMLNGAYFRQSGAGAAS 381
           SPTIYSQGNRF APYNRFRKEVTKHEDAPESEWRHWNWRSEGDM+LNGAYFRQSGAGA S
Sbjct: 301 SPTIYSQGNRFVAPYNRFRKEVTKHEDAPESEWRHWNWRSEGDMLLNGAYFRQSGAGARS 360

Query: 382 SYARANSLSARPSSLVSSITVTAGALNCRKGSRC 416
           SYARA SLSARPSSLV  IT+TAGALNCR+GSRC
Sbjct: 361 SYARAYSLSARPSSLVGDITLTAGALNCRRGSRC 394

BLAST of Bhi06G000499 vs. ExPASy TrEMBL
Match: A0A6J1H707 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111460762 PE=3 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 3.7e-214
Identity = 372/417 (89.21%), Postives = 380/417 (91.13%), Query Frame = 0

Query: 1   MAASSSLSFLFLLLSLSFFFTMSVSTTTSNSSLHSVHQHPHLVVQDVQRSINAS--RRNL 60
           MAAS    F  LLL LSFFFT        +SSL SV Q PHLVVQ+VQRSIN S  RRNL
Sbjct: 53  MAAS---FFFLLLLPLSFFFT--------DSSLASV-QDPHLVVQEVQRSINESRTRRNL 112

Query: 61  GYFSCGTGNPIDDCWRCDSNWATNRKRLADCAIGFGKNAIGGKNGRFYVVTDAGDDDPVN 120
           GY SC TGNPIDDCWRCD NW TNRKRLADCAIGFGKNAIGG+NGRFYVVTDAGDDDP+N
Sbjct: 113 GYLSCRTGNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDAGDDDPLN 172

Query: 121 PRPGTLRHAVIQNEPLWIIFKRDMVIQLTQELVMNSFKTIDGRGASVHVAGGPCIKIHYA 180
           PRPGTLRHAVIQNEPLWIIFKRDMVIQL QELVMNSFKTIDGRGASVHVAGGPCI IHYA
Sbjct: 173 PRPGTLRHAVIQNEPLWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYA 232

Query: 181 TNIIIHGIHIHDCKRGGNANIRDSPQHSGWWTASDGDGVSILGGREIWIDHCSLSNCNDG 240
           TNIIIHGIHIHDCKRGGN NIRDSPQHSGWWT SDGDGVSI GG+ IW+DHCSLSNCNDG
Sbjct: 233 TNIIIHGIHIHDCKRGGNGNIRDSPQHSGWWTISDGDGVSIFGGKNIWVDHCSLSNCNDG 292

Query: 241 LIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYAQDKNMQVTIAFNHFGEGLVQRMPRCR 300
           LIDAIHGSTAITISNNYMTHHDKVMLLGHSDSY QDKNMQVTIAFNHFGEGLVQRMPRCR
Sbjct: 293 LIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCR 352

Query: 301 HGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFAAPYNRFRKEVTKHEDAPESEWRHWN 360
           HGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRF AP  R+RKEVTKHEDAPESEWRHWN
Sbjct: 353 HGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFLAPDVRYRKEVTKHEDAPESEWRHWN 412

Query: 361 WRSEGDMMLNGAYFRQSGAGAASSYARANSLSARPSSLVSSITVTAGALNCRKGSRC 416
           WRSEGDMMLNGAYFRQSGAGAASSYARA SLSARPSSLV SITVTAGALNCR+GSRC
Sbjct: 413 WRSEGDMMLNGAYFRQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 457

BLAST of Bhi06G000499 vs. ExPASy TrEMBL
Match: A0A6J1JPY5 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111487839 PE=3 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 4.1e-213
Identity = 370/417 (88.73%), Postives = 379/417 (90.89%), Query Frame = 0

Query: 1   MAASSSLSFLFLLLSLSFFFTMSVSTTTSNSSLHSVHQHPHLVVQDVQRSINAS--RRNL 60
           MAAS    F  LLL LSFFFT        +SSL SV Q PHLVVQ VQRSIN S  RRNL
Sbjct: 19  MAAS---FFFLLLLPLSFFFT--------DSSLASV-QDPHLVVQQVQRSINESRIRRNL 78

Query: 61  GYFSCGTGNPIDDCWRCDSNWATNRKRLADCAIGFGKNAIGGKNGRFYVVTDAGDDDPVN 120
           GY SC TGNPIDDCWRCD NW TNRKRLADCAIGFGKNAIGG+NGRFY+VTDAGDDDP+N
Sbjct: 79  GYLSCRTGNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYMVTDAGDDDPLN 138

Query: 121 PRPGTLRHAVIQNEPLWIIFKRDMVIQLTQELVMNSFKTIDGRGASVHVAGGPCIKIHYA 180
           PRPGTLRHAVIQNEPLWIIFKRDMVIQL QELVMNSFKTIDGRGASVHVAGGPCI IHYA
Sbjct: 139 PRPGTLRHAVIQNEPLWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYA 198

Query: 181 TNIIIHGIHIHDCKRGGNANIRDSPQHSGWWTASDGDGVSILGGREIWIDHCSLSNCNDG 240
           TNIIIHGIHIHDCKRGGN NIRDSPQHSGWWT SDGDGVSI GG+ IW+DHCSLSNCNDG
Sbjct: 199 TNIIIHGIHIHDCKRGGNGNIRDSPQHSGWWTISDGDGVSIFGGKNIWVDHCSLSNCNDG 258

Query: 241 LIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYAQDKNMQVTIAFNHFGEGLVQRMPRCR 300
           LIDAIHGSTAITISNNYMTHHDKVMLLGHSDSY QDKNMQVTIAFNHFGEGLVQRMPRCR
Sbjct: 259 LIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCR 318

Query: 301 HGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFAAPYNRFRKEVTKHEDAPESEWRHWN 360
           HGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRF AP  R++KEVTKHEDAPESEWRHWN
Sbjct: 319 HGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFLAPDVRYQKEVTKHEDAPESEWRHWN 378

Query: 361 WRSEGDMMLNGAYFRQSGAGAASSYARANSLSARPSSLVSSITVTAGALNCRKGSRC 416
           WRSEGDMMLNGAYFRQSGAGAASSYARA SLSARPSSLV SITVTAGALNCR+GSRC
Sbjct: 379 WRSEGDMMLNGAYFRQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 423

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G27400.12.2e-19077.81Pectin lyase-like superfamily protein [more]
AT4G24780.11.5e-18377.57Pectin lyase-like superfamily protein [more]
AT4G24780.21.5e-18377.57Pectin lyase-like superfamily protein [more]
AT5G63180.18.3e-18272.32Pectin lyase-like superfamily protein [more]
AT1G67750.15.4e-18173.72Pectate lyase family protein [more]
Match NameE-valueIdentityDescription
O245547.0e-19480.30Pectate lyase OS=Zinnia violacea OX=34245 PE=1 SV=1[more]
Q9LTZ03.1e-18977.81Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2[more]
P243966.6e-18476.92Probable pectate lyase P18 OS=Solanum lycopersicum OX=4081 GN=9612 PE=2 SV=1[more]
Q9C5M82.1e-18277.57Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2[more]
Q93Z251.2e-18072.32Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3C8C59.5e-22691.30Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103498129 PE=3 SV=1[more]
A0A0A0KNX31.4e-22490.58Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_5G517820 PE=3 SV=1[more]
A0A5D3CGC72.4e-22192.89Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold249G00560 ... [more]
A0A6J1H7073.7e-21489.21Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111460762 PE=3 SV=1[more]
A0A6J1JPY54.1e-21388.73Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111487839 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 213..234
score: 57.79
coord: 315..334
score: 77.62
coord: 357..381
score: 53.52
coord: 113..138
score: 70.15
coord: 293..312
score: 78.1
coord: 89..106
score: 62.7
coord: 149..165
score: 71.43
coord: 386..409
score: 40.08
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 141..338
e-value: 5.5E-103
score: 358.1
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 150..332
e-value: 2.1E-20
score: 73.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 66..408
e-value: 7.4E-116
score: 388.9
NoneNo IPR availablePANTHERPTHR31683:SF147PECTATE LYASEcoord: 14..412
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 14..412
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 49..409

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi06M000499Bhi06M000499mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity